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https://www.readbyqxmd.com/read/29792953/protective-effect-of-s-nitrosoglutathione-administration-against-hyperglycemia-induced-disruption-of-blood-brain-barrier-is-mediated-by-alterations-in-tight-junction-proteins-and-cell-adhesion-molecules
#1
Aanchal Aggarwal, Inderjit Singh, Rajat Sandhir
Diabetes is associated with increased blood brain barrier (BBB) permeability resulting in neurological deficits. The present study investigated the role of S-nitrosoglutathione (GSNO) on tight junction proteins and cell adhesion molecules in streptozotocin-induced diabetic mice. Diabetes was induced by intraperitoneal injection of streptozotocin (40 mg/kg body weight) for 5 days in mice. GSNO was administered daily (100 μg/kg body weight, orally) for 8 weeks after the induction of diabetes. A significant decline was observed in the cognitive ability of diabetic animals assessed using radial arm maze test...
May 21, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29791586/hypoxia-induced-hyperpermeability-of-rat-glomerular-endothelial-cells-involves-hif-2%C3%AE-mediated-changes-in-the-expression-of-occludin-and-zo-1
#2
Peng-Li Luo, Yan-Jun Wang, Yan-Yan Yang, Jia-Jia Yang
This study aimed to investigate the role of hypoxia-inducible factor-2α (HIF-2α) in the expression of tight junction proteins and permeability alterations in rat glomerular endothelial cells (rGENCs) under hypoxia conditions. The expression level of HIF-2α and tight junction proteins (occludin and ZO-1) in rGENCs were examined following 5% oxygen density exposure at different treatment times. HIF-2α lentivirus transfection was used to knockdown HIF-2α expression. Cells were divided into four groups: 1) control group (rGENCs were cultured under normal oxygen conditions), 2) hypoxia group (rGENCs were cultured under hypoxic conditions), 3) negative control group (rGENCs were infected with HIF-2α lentivirus negative control vectors and cultured under hypoxic conditions), and 4) Len group (rGENCs were transfected with HIF-2α lentivirus and cultured under hypoxic conditions)...
2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29788452/glutamine-alleviates-heat-stress-induced-impairment-of-intestinal-morphology-intestinal-inflammatory-response-and-barrier-integrity-in-broilers
#3
Q J Wu, N Liu, X H Wu, G Y Wang, L Lin
The aim of this study was to investigate the protective effect of glutamine (Gln) on the intestinal morphology, intestinal inflammatory response, and barrier integrity in broilers exposed to high ambient temperature. Three-hundred-sixty 21-d-old Arbor Acres broilers (half male and half female) were randomly allocated to 4 treatment groups in a completely randomized design, each of which included 6 replicates with 15 birds per replicate, for 21 d. The 4 treatment groups were as follows: the control group, in which birds were kept in a thermoneutral room at 22 ± 1°C (no stress, NS; fed a basal diet); the heat stress group (36 ± 1°C for 10 h/d from 08:00 to 18:00 h and 22 ± 1°C for the remaining time, heat stress (HT); fed a basal diet); and heat stress + Gln group (0...
May 17, 2018: Poultry Science
https://www.readbyqxmd.com/read/29786451/annexin-a2-is-a-robo4-ligand-that-modulates-arf6-activation-associated-cerebral-trans-endothelial-permeability
#4
Wenlu Li, Zhigang Chen, Jing Yuan, Zhanyang Yu, Chongjie Cheng, Qiuchen Zhao, Lena Huang, Katherine A Hajjar, Zhong Chen, Eng H Lo, Haibin Dai, Xiaoying Wang
Blood-brain barrier (BBB) disruption in neurological disorders remains an intractable problem with limited therapeutic options. Here, we investigate whether the endothelial cell membrane protein annexin A2 (ANXA2) may play a role in reducing trans-endothelial permeability and maintaining cerebrovascular integrity after injury. Compared with wild-type mice, the expression of cerebral endothelial junctional proteins was reduced in E15.5 and adult ANXA2 knockout mice, along with increased leakage of small molecule tracers...
January 1, 2018: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/29785346/integrated-bioinformatic-changes-and-analysis-of-retina-with-time-in-diabetic-rats
#5
Zekai Cui, Qiaolang Zeng, Yonglong Guo, Shiwei Liu, Jiansu Chen
Diabetic retinopathy (DR) is the most common chronic complication of diabetes. It can cause impaired vision and even blindness. However, the pathological mechanism of DR is still unknown. In the present study, we use bioinformatic analysis to reveal the pathological changes of early DR in a streptozotocin (STZ) induced diabetes rat model. The dataset GSE28831 was downloaded from the Gene Expression Omnibus (GEO) database. To clarify the pathological mechanism of early DR, genes which were up-regulated (UP group) or down-regulated (DOWN group) over time were identified...
2018: PeerJ
https://www.readbyqxmd.com/read/29783653/avian-influenza-virus-subtype-h9n2-affects-intestinal-microbiota-barrier-structure-injury-and-inflammatory-intestinal-disease-in-the-chicken-ileum
#6
Hongxin Li, Xiaolin Liu, Feiyang Chen, Kejing Zuo, Che Wu, Yiming Yan, Weiguo Chen, Wencheng Lin, Qingmei Xie
Avian influenza virus subtype H9N2 (H9N2 AIV) has caused significant losses to the poultry industry due to the high mortality associated with secondary infections attributable to E. coli . This study tries to address the underlying secondary mechanisms after H9N2 AIV infection. Initially, nine day-old specific pathogen-free chickens were assigned to control (uninfected) and H9N2-infected groups, respectively. Using Illumina sequencing, histological examination, and quantitative real-time PCR, it was found that H9N2 AIV caused intestinal microbiota disorder, injury, and inflammatory damage to the intestinal mucosa...
May 18, 2018: Viruses
https://www.readbyqxmd.com/read/29782355/inhibition-of-vascular-endothelial-cell-leak-following-escherichia-coli-attachment-in-an-experimental-model-of-sepsis
#7
Tony M McHale, Carolina D Garciarena, Robert P Fagan, Stephen G J Smith, Ignacio Martin-Loches, Gerard F Curley, Fidelma Fitzpatrick, Steve W Kerrigan
OBJECTIVES: The vascular endothelium is a major target of sepsis-induced events, and endothelial activation accounts for much of the pathology of sepsis. Urinary tract infections and pneumonia caused by Escherichia coli are among of the most common infections causing sepsis in both community and hospital settings. Currently, there are no approved drugs on the market to treat the underlying pathophysiology of sepsis. The aim of this study is to elucidate the molecular mechanism by which E...
May 18, 2018: Critical Care Medicine
https://www.readbyqxmd.com/read/29782346/claudins-and-nephrolithiasis
#8
Allein Plain, R Todd Alexander
PURPOSE OF REVIEW: The greatest risk factor for kidney stone formation is increased urinary calcium excretion. Most filtered calcium is reabsorbed from the proximal tubule and the thick ascending limb (TAL) of Henle's loop via a paracellular pathway. Claudins are tight junction proteins that confer the permeability properties of an epithelium. We review the contribution of renal claudins to nephron calcium permeability and how perturbations in these pathways cause alterations in tubular calcium transport, hypercalciuria, nephrocalcinosis, or nephrolithiasis...
May 17, 2018: Current Opinion in Nephrology and Hypertension
https://www.readbyqxmd.com/read/29782323/role-of-blood-brain-barrier-in-alzheimer-s-disease
#9
Zhiyou Cai, Pei-Feng Qiao, Cheng-Qun Wan, Min Cai, Nan-Kai Zhou, Qin Li
The blood-brain barrier (BBB) is involved in the pathogenesis of Alzheimer's disease (AD). BBB is a highly selective semipermeable structural and chemical barrier which ensures a stable internal environment of the brain and prevents foreign objects invading the brain tissue. BBB dysfunction induces the failure of Aβ transport from brain to the peripheral circulation across the BBB. Especially, decreased levels of LRP-1 (low density lipoprotein receptor-related protein 1) and increased levels of RAGE (receptor for advanced glycation endproducts) at the BBB can cause the failure of Aβ transport...
May 16, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29781811/spleen-derived-classical-monocytes-mediate-lung-ischemia-reperfusion-injury-through-il-1%C3%AE
#10
Hsi-Min Hsiao, Ramiro Fernandez, Satona Tanaka, Wenjun Li, Jessica H Spahn, Stephen Chiu, Mahzad Akbarpour, Daniel Ruiz-Perez, Qiang Wu, Cem Turam, Davide Scozzi, Tsuyoshi Takahashi, Hannah P Luehmann, Varun Puri, G R Scott Budinger, Alexander S Krupnick, Alexander V Misharin, Kory J Lavine, Yongjian Liu, Andrew E Gelman, Ankit Bharat, Daniel Kreisel
Ischemia-reperfusion injury, a form of sterile inflammation, is the leading risk factor for both short-term mortality following pulmonary transplantation and chronic lung allograft dysfunction. While it is well recognized that neutrophils are critical mediators of acute lung injury, processes that guide their entry into pulmonary tissue are not well understood. Here, we found that CCR2+ classical monocytes are necessary and sufficient for mediating extravasation of neutrophils into pulmonary tissue during ischemia-reperfusion injury following hilar clamping or lung transplantation...
May 21, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29778535/lysophosphatidic-acid-receptor-lpa-6-regulates-endothelial-blood-brain-barrier-function-implication-of-hepatic-encephalopathy
#11
Kayo Masago, Yasuyuki Kihara, Keisuke Yanagida, Fumie Hamano, Shinsuke Nakagawa, Masami Niwa, Takao Shimizu
Cerebral edema is a life-threatening neurological condition characterized by brain swelling due to accumulation of excess fluid both intracellularly and extracellularly. Fulminant hepatic failure (FHF) develops cerebral edema by disrupting blood brain barrier (BBB). However, the mechanisms by which mediator induces brain edema in FHF remain to be elucidated. Here, we assessed a linkage between brain edema and lysophosphatidic acid (LPA) signaling by utilizing an animal model of FHF and in vitro BBB model. Azoxymethane-treated mice developed FHF and hepatic encephalopathy, associated with higher autotaxin (ATX) activities in serum than controls...
May 17, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777753/characterization-of-impaired-cerebrovascular-structure-in-app-ps1-mouse-brains
#12
Kee-Chan Ahn, Cameron R Learman, Gary L Dunbar, Panchanan Maiti, Won-Cheoul Jang, Hyeon-Cheol Cha, Mee-Sook Song
Alzheimer's disease (AD) is defined by senile plaques, tauopathy and neuronal cell death in specific area of the brain. Recent studies suggest that neurovascular dysfunction may be an integral part of AD pathogenesis, contributing to the onset and development of AD pathologies such as neuronal death, inflammatory response, and breakdown of blood brain barrier (BBB). In addition, vascular complications caused by age-related metabolic diseases such as diabetes and high blood pressure have high incidence in development of dementia and AD...
May 16, 2018: Neuroscience
https://www.readbyqxmd.com/read/29776350/increased-systemic-zonula-occludens-1-associated-with-inflammation-and-independent-biomarker-in-patients-with-hepatocellular-carcinoma
#13
Amit Kumar Ram, Biju Pottakat, Balasubramaniyan Vairappan
BACKGROUND: Hepatocellular carcinoma (HCC) is a frequent type of primary liver cancer, and its prevalence is increasing worldwide. Indeed, the underlying molecular mechanism is not well understood. Previous studies have shown evidence that tight junction (TJ) components were correlated with carcinogenesis and tumor development. Our aims were to determine the serum levels of tight junction protein Zonula Occludens (ZO)-1 and an inflammatory marker such as high-sensitive C-reactive protein (hs-CRP) in HCC patients compared to healthy volunteers and also to identify the association between ZO-1 and inflammation in HCC...
May 18, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29775175/hiv-induced-matrix-metalloproteinase-9-activation-through-mitogen-activated-protein-kinase-signalling-promotes-hsv-1-cell-to-cell-spread-in-oral-epithelial-cells
#14
Irna Sufiawati, Sharof M Tugizov
We have shown that cell-free HIV-1 and viral proteins tat and gp120 activate mitogen-activated protein kinases (MAPKs) in tonsil epithelial cells, disrupting their tight and adherens junctions. This causes liberation of the HSV-1 receptor nectin-1 from assembled adherens junctions, leading to promotion of HSV-1 infection and spread. In the present study, we show that HIV-associated activation of MAPK leads to upregulation of transcription factor NF-κB and matrix metalloproteinase-9 (MMP-9). This induces the disruption of tight and adherens junctions, increasing HSV-1 cell-to-cell spread...
May 18, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29773101/-microvesicles-derived-from-lps-induced-microglia-aggravate-the-injury-of-tight-junction-in-rat-brain-microvascular-endothelial-cells-under-oxygen-glucose-deprivation
#15
Yanni Lyu, Longsheng Fu, Yanming Li, Jinhua Wen, Xiaohua Wei, Yisong Qian
Objective To study the effect of the administration of the circulating microvesicles (MV) obtained from lipopolysaccharide (LPS)-stimulated microglia supernatant into rat brain endothelial cells (RBECs) on the injury of tight junction in RBECs under the condition of oxygen-glucose deprivation (OGD) as well as the underlying mechanism. Methods The circulating MV was isolated from the supernatant of microglia stimulated with LPS 1 mg/L for 24 hours and subjected to morphological identification. The expression of miR-27a in MV was detected by real-time PCR...
March 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29766204/adiponectin-administration-alleviates-dss-induced-colonic-inflammation-in-caco-2-cells-and-mice
#16
Qin Zhao, Yang Liu, Lei Tan, Liyong Yan, Xiuli Zuo
BACKGROUND: Adiponectin, a protein hormone produced by adipose tissues, exhibits anti-inflammatory functions in various models. This study was investigated the effects of adiponectin on dextran sodium sulfate (DSS)-colonic injury, inflammation, apoptosis, and intestinal barrier dysfunction in Caco-2 cell and mice. MATERIALS AND METHODS: The results showed that DSS caused inflammatory response and intestinal barrier dysfunction in vitro and in vivo. Adiponectin injection alleviated colonic injury and rectal bleeding in mice...
May 15, 2018: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/29765546/proteomic-screening-identifies-the-zonula-occludens-protein-zo-1-as-a-new-partner-for-adam12-in-invadopodia-like-structures
#17
Bassil Dekky, Michael Ruff, Dominique Bonnier, Vincent Legagneux, Nathalie Théret
The epithelial mesenchymal transition (EMT) is a key process for cancer cell invasion and migration. This complex program whereby epithelial tumor cells loose polarity and acquire mesenchymal phenotype is driven by the regulation of cell-cell adhesion and cell-substrate interactions. We recently described the association of ADAM12 with EMT and we now use immunoprecipitation and proteomic approaches to identify interacting partners for ADAM12 during EMT. We identify twenty proteins that are involved in molecular mechanisms associated with adhesion/invasion processes...
April 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29762159/vitamin-d-in-inflammatory-bowel-disease-more-than-just-a-supplement
#18
John Gubatan, Alan C Moss
PURPOSE OF REVIEW: The aim of this review is to explore the protective role of vitamin D on the gastrointestinal tract, summarize the epidemiology of vitamin D deficiency in inflammatory bowel disease (IBD), and highlight recent studies examining the impact of low vitamin D and vitamin D supplementation on IBD clinical outcomes. RECENT FINDINGS: Vitamin D protects the gut barrier by regulating tight junction proteins and inhibiting intestinal apoptosis. Vitamin D enhances innate immunity by inducing antimicrobial peptides and regulates adaptive immunity by promoting anti-inflammatory T cells and cytokines...
May 11, 2018: Current Opinion in Gastroenterology
https://www.readbyqxmd.com/read/29760423/simultaneous-amelioratation-of-colitis-and-liver-injury-in-mice-by-bifidobacterium-longum-lc67-and-lactobacillus-plantarum-lc27
#19
Se-Eun Jang, Jin-Ju Jeong, Jeon-Kyung Kim, Myung Joo Han, Dong-Hyun Kim
Disturbances in the gut microbiota composition are associated with chronic inflammatory diseases of the intestine and the liver. In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation. Here, we investigated their effects on 2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis and liver damage in mice. First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100...
May 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29755347/honokiol-a-polyphenol-natural-compound-attenuates-cisplatin-induced-acute-cytotoxicity-in-renal-epithelial-cells-through-cellular-oxidative-stress-and-cytoskeleton-modulations
#20
Tse-En J Wang, Hung-Ting Liu, Yu-Hua Lai, Tong-Rong Jan, Naohiro Nomura, Hui-Wen Chang, Chi-Chung Chou, Ya-Jane Lee, Pei-Shiue J Tsai
Cisplatin is a potent anti-cancer drug that has been widely used in the treatment of various cancers; however, cisplatin administration results in severe nephrotoxicity and impedes its clinical applications. In this study, we showed that honokiol, a polyphenol constituent extracted from Magnolia officinalis exhibited a short-term protective effect against cisplatin-induced damages in renal epithelial cells in vitro . The protective effects of honokiol were resulted from the combination of (1) reduced cellular oxidative stress ranging from 53 to 32% reduction during a 24-h incubation, (2) the maintenance of cellular antioxidant capacity and (3) the stabilization of cytoskeletal structure of the kidney epithelial cells...
2018: Frontiers in Pharmacology
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