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biomarkers in stress

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https://www.readbyqxmd.com/read/29045922/the-impact-of-expired-commercial-drugs-on-non-target-marine-species-a-case-study-with-the-use-of-a-battery-of-biomarkers-in-hemocytes-of-mussels
#1
Nektarios Politakis, Alexia Belavgeni, Ioanna Efthimiou, Nikolina Charalampous, Chara Kourkouta, Stefanos Dailianis
The present study investigated the effects of two expired commercial medicines, like Buscopan Plus and Mesulid, commonly classified as household medical wastes, on hemocytes of mussel Mytilus galloprovincialis. Mussel hemocytes' lysosomal membrane stability (in terms of neutral red retention assay), superoxide anions (O2(·)(-)) and nitric oxides (NO, in terms of nitrites) production, lipid peroxidation (in terms of malondialdehyde/MDA content) and the formation of nuclear abnormalities (using the micronucleus/MN assay) were assessed in hemocytes of mussels treated for 7 days with appropriate amounts of each drug (the concentrations of active substances were considered in each case, due to the absence of data related with the excipients) as well as in hemocytes of post-treated/recovered mussels (7 days post-treatment/recovery period)...
October 14, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29045571/zinc-induced-metallothionein-in-centenarian-offspring-from-a-large-european-population-the-mark-age-project
#2
Robertina Giacconi, Laura Costarelli, Francesco Piacenza, Andrea Basso, Alexander Bürkle, Maria Moreno-Villanueva, Tilman Grune, Daniela Weber, Wolfgang Stuetz, Efstathios S Gonos, Christiane Schön, Beatrix Grubeck-Loebenstein, Ewa Sikora, Olivier Toussaint, Florence Debacq-Chainiaux, Claudio Franceschi, Antti Hervonen, Eline Slagboom, Fabio Ciccarone, Michele Zampieri, Paola Caiafa, Eugène Jansen, Martijn E T Dollé, Nicolle Breusing, Eugenio Mocchegiani, Marco Malavolta
Metallothionein (MT) family are cysteine-rich proteins that regulate zinc (Zn) homeostasis and protect against oxidative damage. Studies in transgenic mice have shown that MT favourably influence longevity, although their role in human aging is not completely understood.Within the European multicenter study MARK-AGE, we analysed MT induction after Zn treatment in peripheral blood mononuclear cells (PBMCs) and its relation with redox biomarkers in 2936 age-stratified subjects (35-75 years) including the general population (RASIG), centenarian offspring (GO) and their spouses (SGO)...
October 17, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29041000/genetic-variations-of-oxidative-stress-related-genes-alox5-alox5ap-and-mpo-modulate-ischemic-stroke-susceptibility-through-main-effects-and-epistatic-interactions-in-a-chinese-population
#3
Ding Liu, Lei Liu, Zhi Song, Zhongyang Hu, Jia Liu, Deren Hou
BACKGROUND/AIMS: To investigate the roles of the oxidative stress related-genes ALOX5, ALOX5AP and MPO in ischemic stroke susceptibility in the Han Chinese population. METHODS: A total of 351 ischemic stroke patients and 417 controls were recruited. The ALOX5 rs10900213, ALOX5AP rs4293222 and MPO rs2107545 gene polymorphisms were genotyped. RESULTS: We identified that rs2107545 of MPO gene was significantly associated with ischemic stroke susceptibility after adjusting for covariates...
October 17, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29040278/pg-metrics-a-chemometric-based-approach-for-classifying-bacterial-peptidoglycan-data-sets-and-uncovering-their-subjacent-chemical-variability
#4
Keshav Kumar, Akbar Espaillat, Felipe Cava
Bacteria cells are protected from osmotic and environmental stresses by an exoskeleton-like polymeric structure called peptidoglycan (PG) or murein sacculus. This structure is fundamental for bacteria's viability and thus, the mechanisms underlying cell wall assembly and how it is modulated serve as targets for many of our most successful antibiotics. Therefore, it is now more important than ever to understand the genetics and structural chemistry of the bacterial cell walls in order to find new and effective methods of blocking it for the treatment of disease...
2017: PloS One
https://www.readbyqxmd.com/read/29039553/proteomic-analysis-of-serum-deprivation-in-tongue-squamous-cell-carcinoma
#5
Junfeng Zhang, Wei Dong, Yufen Meng, Miao Jiang, Zhen Zhan
The occurrence of tongue squamous cell carcinoma (TSCC) is closely correlated with serum components; however, the detailed mechanism remains to be fully elucidated. Proteomic analysis contributed to the discovery of potential biomarkers and provided an insight into TSCC at a molecular level. The present study investigated the effect of serum deprivation on the Tca‑8113 TSCC cell line through protein profiling using two‑dimensional gel electrophoresis and mass spectrometry, with the aim of improving TSCC diagnosis...
October 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039505/characterization-of-acid%C3%A2-tolerance%C3%A2-associated-small-rnas-in-clinical-isolates-of-streptococcus-mutans-potential-biomarkers-for-caries-prevention
#6
Wenhui Zhu, Shanshan Liu, Peilin Zhuang, Jia Liu, Yan Wang, Huancai Lin
Streptococcus mutans is a cariogenic bacterium that contributes to dental caries due to its ability to produce lactic acid, which acidifies the local environment. The potential of S. mutans to respond to environmental stress and tolerate low pH is essential for its survival and predominance in caries lesions. Small noncoding RNAs (sRNAs) have been reported to be involved in bacterial stress and virulence. Few studies have investigated the sRNAs of S. mutans and the function of these sRNAs remains to be elucidated...
October 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039102/analysis-of-pro-inflammatory-cytokine-and-type-ii-interferon-induction-by-nanoparticles
#7
Timothy M Potter, Barry W Neun, Jamie C Rodriguez, Anna N Ilinskaya, Marina A Dobrovolskaia
Cytokines, chemokines, and interferons are released by the immune cells in response to cellular stress, damage and/or pathogens, and are widely used as biomarkers of inflammation. Certain levels of cytokines are needed to stimulate an immune response in applications such as vaccines or immunotherapy where immune stimulation is desired. However, undesirable elevation of cytokine levels, as may occur in response to a drug or a device, may lead to severe side effects such as systemic inflammatory response syndrome or cytokine storm...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29039061/erod-activity-chromosomal-damage-and-oxidative-stress-in-response-to-contaminants-exposure-in-tree-swallow-tachycineta-bicolor-nestlings-from-great-lakes-areas-of-concern
#8
Thomas W Custer, Christine M Custer, Paul M Dummer, Emilie Bigorgne, Elias M Oziolor, Natalie Karouna-Renier, Sandra Schultz, Richard A Erickson, Kevin Aagaard, Cole W Matson
Tree swallow, Tachycineta bicolor, nestlings were collected from 60 sites in the Great Lakes, which included multiple sites within 27 Areas of Concern (AOCs) and six sites not listed as AOCs from 2010 to 2014. Nestlings, approximately 12 days-of-age, were evaluated for ethoxyresorufin-O-dealkylase (EROD) activity, chromosomal damage, and six measures of oxidative stress. Data on each of these biomarkers were divided into four equal numbered groups from the highest to lowest values and the groups were compared to contaminant concentrations using multivariate analysis...
October 16, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/29038346/atr-is-a-therapeutic-target-in-synovial-sarcoma
#9
Samuel E Jones, Emmy D G Fleuren, Jessica Frankum, Asha Konde, Chris T Williamson, Dragomir B Krastev, Helen N Pemberton, James Campbell, Aditi Gulati, Richard Elliott, Malini Menon, Joanna L Selfe, Rachel Brough, Stephen J Pettitt, Wojciech Niedzwiedz, Winette T A van der Graaf, Janet Shipley, Alan Ashworth, Christopher J Lord
Synovial sarcoma (SS) is an aggressive soft-tissue malignancy characterised by expression of SS18-SSX fusions, where treatment options are limited. To identify therapeutically actionable genetic dependencies in SS, we performed a series of parallel, high-throughput small interfering RNA (siRNA) screens and compared genetic dependencies in SS tumor cells to those in >130 non-SS tumour cell lines. This approach revealed a reliance of SS tumor cells upon the DNA damage response serine/threonine protein kinase ATR...
October 16, 2017: Cancer Research
https://www.readbyqxmd.com/read/29038153/prospective-validation-of-a-biomarker-based-rule-out-strategy-for-functionally-relevant-coronary-artery-disease
#10
Joan E Walter, Ursina Honegger, Christian Puelacher, Deborah Mueller, Max Wagener, Nicolas Schaerli, Ivo Strebel, Raphael Twerenbold, Jasper Boeddinghaus, Thomas Nestelberger, Lorraine Sazgary, Stella Marbot, Jeanne du Fay de Lavallaz, Christoph Kaiser, Stefan Osswald, Damian Wild, Katharina Rentsch, Michael Zellweger, Tobias Reichlin, Christian Mueller
BACKGROUND: This study aimed to prospectively advance a rule-out strategy for functionally significant coronary artery disease (CAD) by use of high-sensitivity cardiac troponin I (hs-cTnI) from bench to bedside, by application of a 3-step approach: validation in serum, correlation in plasma, and application on a clinical platform. METHODS: Patients without known CAD referred for rest/stress myocardial perfusion single-photon emission tomography/computer tomography (MPI-SPECT/CT) were assigned to 3 consecutive cohorts: validation, correlation, and application...
October 16, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/29038020/the-ca-2-camkk2-axis-mediates-the-telbivudine-induces-upregulation-of-creatine-kinase-implications-for-mechanism-of-antiviral-nucleoside-analogs-side-effect
#11
Long Jianfei, Wang Min, Ma Chunlai, Chen Bicui, Zhang Jiming, Wang Bin
Telbivudine (LdT), a widely prescribed anti-hepatitis B virus (HBV) drug for the treatment of chronic Hepatitis B (CHB), causes adverse reactions ranging from creatine kinase (CK) elevation to myopathy. The purpose of this study was to explore the mechanism(s) of LdT induced CK elevation. The effects of LdT on mitochondrial morphology and proteins (TK2 and β-actin), oxidative stress, intracellular Ca(2+) levels, Ca(2+)-related signaling pathway (CaMKK2/AMPK), and Ca(2+)-related biomarkers such as superoxide dismutase (SOD) and malondialdehyde (MDA) were assessed in human skeletal muscle cells (HSKMCs)...
October 13, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29037107/first-in-man-evaluation-of-124-i-pgn650-a-pet-tracer-for-detecting-phosphatidylserine-as-a-biomarker-of-the-solid-tumor-microenvironment
#12
Richard Laforest, Farrokh Dehdashti, Yongjian Liu, Jennifer Frye, Sarah Frye, Hannah Luehmann, Deborah Sultan, Joseph S Shan, Bruce D Freimark, Barry A Siegel
PURPOSE: PGN650 is a F(ab')2 antibody fragment that targets phosphatidylserine (PS), a marker normally absent that becomes exposed on tumor cells and tumor vasculature in response to oxidative stress and increases in response to therapy. PGN650 was labeled with (124)I to create a positron emission tomography (PET) agent as an in vivo biomarker for tumor microenvironment and response to therapy. In this phase 0 study, we evaluated the pharmacokinetics, safety, radiation dosimetry, and tumor targeting of this tracer in a cohort of patients with cancer...
January 2017: Molecular Imaging
https://www.readbyqxmd.com/read/29035092/vitamin-d-supplementation-and-non-alcoholic-fatty-liver-disease-a-critical-and-systematic-review-of-clinical-trials
#13
Nasrin Sharifi, Reza Amani
Previous observational studies have found a relationship between vitamin D deficiency and non-alcoholic fatty liver disease (NAFLD). However, this type of study could not show the causal relationship between these two conditions. Therefore, we systematically and critically reviewed the available clinical trials to elucidate such relationship. We searched databases such as Medline, Scopus and Cochrane to identify the clinical trials that assessed the effects of vitamin D supplementation in adults with NAFLD...
October 16, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29032190/transient-increase-in-sapp%C3%AE-secretion-in-response-to-a%C3%AE-1-42-oligomers-an-attempt-of-neuronal-self-defense
#14
Christiane Rose, Emilie Dorard, Mickael Audrain, Lucie Gorisse-Hussonnois, Nathalie Cartier, Jérome Braudeau, Bernadette Allinquant
Amyloid precursor protein (APP), a key molecule of Alzheimer disease, is metabolized in 2 antagonist pathways generating the soluble APP alpha (sAPPα) having neuroprotective properties and the beta amyloid (Aβ) peptide at the origin of neurotoxic oligomers, particularly Aβ1-42. Whether extracellular Aβ1-42 oligomers modulate the formation and secretion of sAPPα is not known. We report here that the addition of Aβ1-42 oligomers to primary cortical neurons induced a transient increase in α-secretase activity and secreted sAPPα 6-9 hours later...
September 19, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/29032143/effects-of-cr-vi-on-ca-2-atpase-activity-in-the-earthworm-eisenia-andrei
#15
Marta Boeri, Caterina Oliveri, Corrado Camurati, Aldo Viarengo, Susanna Sforzini
The effect of Cr(VI) as a soil contaminant on the edaphic worm Eisenia andrei was studied by evaluating the activity of Ca(2+)-ATPase in the intestinal mucosa. In eukaryotes, Ca(2+)-ATPase is a key mediator of cell signaling although comparatively little is known about its activity in earthworms. Size and anatomical constraints (i.e. small and complex) led us to develop and optimize a cyto-biochemical method to measure Ca(2+)-ATPase activity in earthworms. The principal site of enzyme activity was found to be the post clitellar intestinal tract; immunohistochemistry then identified plasma membrane Ca(2+)-ATPase (PMCA ATPase) in the apical area of the intestinal epithelium...
October 11, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29032117/salvia-miltiorrhiza-protects-adenine-induced-chronic-renal-failure-by-regulating-the-metabolic-profiling-and-modulating-the-nadph-oxidase-ros-erk-and-tgf-%C3%AE-smad-signaling-pathways
#16
Hongdie Cai, Shulan Su, Yonghui Li, Huiting Zeng, Zhenhua Zhu, Jianming Guo, Yue Zhu, Sheng Guo, Li Yu, Dawei Qian, Yuping Tang, Jinao Duan
ETHNOPHARMACOLOGICAL RELEVANCE: Chronic renal failure (CRF) is defined as a progressive and irreversible loss of renal function and associated with inflammation and oxidative stress. Salvia miltiorrhiza (SM) is an important Chinese herb used in traditional Chinese medicine with notable effects on CRF. It significantly inhibited the elevation of serum creatinine (Scr), blood urea nitrogen (BUN) and urine protein (UP) in adenine-induced CRF rats, while the action mechanism need to be illuminated deeply...
October 11, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29032097/comparative-assessment-of-the-adverse-outcome-of-wastewater-effluents-by-integrating-oxidative-stress-and-histopathological-alterations-in-endemic-fish
#17
Palas Samanta, Hyungjoon Im, Jisu Yoo, Hwanggoo Lee, Nan-Young Kim, Wonky Kim, Soon-Jin Hwang, Woo-Keun Kim, Jinho Jung
This study evaluated the adverse effect of wastewater effluents on three fish species, Carassius auratus, Zacco platypus, and Zacco koreanus, collected in the Eungcheon, Mihocheon, and Busocheon streams, respectively. Fish gills, liver, and kidneys from the mixing zone (MZ) and sites upstream (US) and downstream (DS) of the MZ were analyzed for oxidative stress responses and histology. Catalase and glutathione S-transferase activity was significantly higher at MZ and DS than from US (p<0.05), indicating induction of antioxidant defense mechanisms...
October 8, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29031784/isobarictags-for-relative-and-absolute-quantitation-itraq-based-proteomics-for-the-investigation-of-the-effect-of-huganqingzhi-on-non-alcoholic-fatty-liver-disease-in-rats
#18
Fan Xia, Xiaorui Yao, Waijiao Tang, Chunxin Xiao, Miaoting Yang, Benjie Zhou
ETHNOPHARMACOLOGICAL RELEVANCE: Hugan Qingzhi tablet (HQT), a traditional Chinese medicine formula has been adopted for preventing and treating nonalcoholic fatty liver disease (NAFLD). AIM: In order to explore the anti-NAFLD mechanisms of HQT, iTRAQ-based proteomics was employed to investigate the expression profiles of proteins in NAFLD rats induced by high-fat diet after HQT treatment. MATERIALS AND METHODS: The NAFLD rat model was administration of high-fat diet (HFD) for 12weeks and administering HQT in a daily basis to the HFD groups...
October 12, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29031614/high-mobility-group-box-1-is-a-novel-pathogenic-factor-and-a-mechanistic-biomarker-for-epilepsy
#19
Teresa Ravizza, Gaetano Terrone, Alessia Salamone, Federica Frigerio, Silvia Balosso, Daniel J Antoine, Annamaria Vezzani
Approximately 30% of epilepsy patients experience seizures that are not controlled by the available drugs. Moreover, these drugs provide mainly a symptomatic treatment since they do not interfere with the disease's mechanisms. A mechanistic approach to the discovery of key pathogenic brain modifications causing seizure onset, recurrence and progression is instrumental for designing novel and rationale therapeutic interventions that could modify the disease course or prevent its development. In this regard, increasing evidence shows that neuroinflammation is a pathogenic factor in drug-resistant epilepsies...
October 12, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29031397/modulation-of-the-biomarkers-of-inflammation-and-oxidative-stress-by-ruminant-trans-fatty-acids-and-dairy-proteins-in-vascular-endothelial-cells-huvec
#20
Marine S Da Silva, Jean-François Bilodeau, Jessica Larose, Karine Greffard, Pierre Julien, Olivier Barbier, Iwona Rudkowska
This study aimed to determine whether dairy macronutrients alter markers of inflammation and oxidative stress in endothelial cells. Human endothelial cells (HUVEC) were treated with ruminant trans fatty acids (rTFA), either trans-vaccenic acid (tVA) or trans-palmitoleic acid (tPA), whey protein hydrolysate, leucine or combinations of rTFA and dairy protein compounds. Industrial TFA elaidic acid (EA) was also investigated and compared with rTFA. Inflammatory prostaglandins (PG) and F2-isoprostanes (F2-isoP) isomers, markers of oxidative stress, were assessed in cell supernatants by LC-MS/MS...
November 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
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