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MicroRNA AND sepsis

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https://www.readbyqxmd.com/read/29123608/the-effects-of-microrna-34a-regulating-notch-1-nf-%C3%AE%C2%BAb-signaling-pathway-on-lipopolysaccharide-induced-human-umbilical-vein-endothelial-cells
#1
Yun Ge, Man Huang, Yue-Feng Ma
BACKGROUND: Notch-1/NF-κB signaling plays a key role in the cecal ligation and puncture (CLP)-induced sepsis. This study aims to investigate the intervention effects of microRNA-34a (miR-34a) lentivirus regulating Notch-1/NF-κB signaling pathway on lipopolysaccharide (LPS)-induced human umbilical vein endothelial cells (HUVEC). METHODS: HUVEC were divided into four groups as the following: they were infected with negative control lentivirus (NC group) or miR-34a lentivirus (OE group); LPS (1 μg/mL) was added on the third day on the basis of NC group and OE group for 24 hours (NC+LPS group or OE+LPS group)...
2017: World Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28947753/tnf-%C3%AE-mrna-is-negatively-regulated-by-microrna-181a-5p-in-maturation-of-dendritic-cells-induced-by-high-mobility-group-box-1-protein
#2
Jing Zhu, Fu-Li Wang, Hai-Bin Wang, Ning Dong, Xiao-Mei Zhu, Yao Wu, Yong-Tao Wang, Yong-Ming Yao
Dendritic cell (DC) can be stimulated by both exogenous pathogen-associated molecular patterns (PAMPs) such as lipopolysaccharide (LPS) and endogenous damage-associated molecular patterns (DAMPs) such as high mobility group box-1 protein (HMGB1). MicroRNAs (miRNAs) act as post-transcriptional fine tuners of mRNA. Studies have focused mostly on the potential role of miRNAs in DCs maturation triggered by PAMPs, especially LPS, however, little is known about the regulatory mechanism underlying the effects of miRNAs in DC maturation mediated by DAMPs, including HMGB1...
September 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28934717/expression-of-c-ebp%C3%AE-in-myeloid-progenitors-during-sepsis-promotes-immunosuppression
#3
Jun Dai, Ajinkya Kumbhare, Dima Youssef, Zhi Q Yao, Charles E McCall, Mohamed El Gazzar
Sepsis-induced myeloid-derived suppressor cells (MDSCs) contribute to immunosuppression associated with sepsis. We reported that the CCAAT enhancer-binding protein C/EBPβ activates microRNA (miR)-21 and miR-181b expressions, which induce transcription factor NFI-A to support the generation and expansion of MDSCs in the bone marrow and spleens of septic mice. Here, using a conditional knockout mouse model lacking C/EBPβ in the myeloid lineage, we find that without C/EBPβ, myeloid progenitor cells could not express miR-21 or miR-181b, and ectopic expression of C/EBPβ in the C/EBPβ-deficient myeloid progenitors activated the expression of the two miRNAs...
November 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28901393/lps%C3%A2-induced-downregulation-of-microrna%C3%A2-204-211-upregulates-and-stabilizes-angiopoietin%C3%A2-1-mrna-in-ea-hy926-endothelial-cells
#4
Yijun Zhang, Caixia Gan, Jiangbo Zhang, Dong Chen
Angiopoietin‑1 (ANG‑1), a ligand of the endothelial cell‑specific TIE2 surface receptor, acts in a complementary and coordinated manner with vascular endothelial growth factor during the process of angiogenesis. ANG‑1 can be used as a clinically informative biomarker of disease severity and outcome in severe sepsis. The epithelium‑specific Ets transcription factor 1 can activate ANG‑1 transcription in the setting of inflammation; however, relatively little is known about the regulation of ANG‑1 by microRNAs (miRs)...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28856588/whole-blood-microrna-markers-are-associated-with-acute-respiratory-distress-syndrome
#5
Zhaozhong Zhu, Liming Liang, Ruyang Zhang, Yongyue Wei, Li Su, Paula Tejera, Yichen Guo, Zhaoxi Wang, Quan Lu, Andrea A Baccarelli, Xi Zhu, Ednan K Bajwa, B Taylor Thompson, Guo-Ping Shi, David C Christiani
BACKGROUND: MicroRNAs (miRNAs) can play important roles in inflammation and infection, which are common manifestations of acute respiratory distress syndrome (ARDS). We assessed if whole blood miRNAs were potential diagnostic biomarkers for human ARDS. METHODS: This nested case-control study (N = 530) examined a cohort of ARDS patients and critically ill at-risk controls. Whole blood miRNA profiles and logistic regression analyses identified miRNAs correlated with ARDS...
August 30, 2017: Intensive Care Medicine Experimental
https://www.readbyqxmd.com/read/28841455/cardiovascular-dysfunction-in-sepsis-at-the-dawn-of-emerging-mediators
#6
REVIEW
Consolato Sergi, Fan Shen, David W Lim, Weiyong Liu, Mingyong Zhang, Brian Chiu, Vijay Anand, Ziyong Sun
Subcellular dysfunction and impaired metabolism derived from the complex interaction of cytokines and mediators with cellular involvement are on the basis of the cardiovascular response to sepsis. The lethal consequences of an infection are intimately related to its ability to spread to other organ sites and the immune system of the host. About one century ago, William Osler (1849-1919), a Canadian physician, remarkably defined the sequelae of the host response in sepsis: "except on few occasions, the patient appears to die from the body's response to infection rather than from it...
November 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28820886/circulating-mirnas-in-sepsis-a-network-under-attack-an-in-silico-prediction-of-the-potential-existence-of-mirna-sponges-in-sepsis
#7
Catalin Vasilescu, Mihnea Dragomir, Mihai Tanase, Dana Giza, Raluca Purnichescu-Purtan, Meng Chen, Sai-Ching Jim Yeung, George A Calin
Biomarkers based on the molecular mechanism of sepsis are important for timely diagnosis and treatment. A large panel of small non-coding microRNAs was reported to modulate the immune response in sepsis but have not been tested in clinical practice. Large-scale identification of microRNA networks in sepsis might reveal a new biological mechanism that can be also targeted by gene therapy. Therefore, the main objective of this study is to perform a comparison of the miRNA network between septic patients and healthy controls...
2017: PloS One
https://www.readbyqxmd.com/read/28759810/mir-130b-attenuates-vascular-inflammation-via-negatively-regulating-tumor-progression-locus-2-tpl2-expression
#8
Peng Wang, Xue Zhang, Fulun Li, Kai Yuan, Maoran Li, Jiwei Zhang, Bin Li, Wei Liang
Endothelial cell (EC) activation and dysfunction have been linked to a wide variety of vascular inflammatory diseases. However, the role of microRNAs in EC activation and inflammation remains largely unknown. In this study, we found that miR-130b was significantly decreased in human umbilical vein endothelial cells (HUVECs) after lipopolysaccharides (LPS) treatment. Forced expression of miR-130b inhibited the LPS-induced activation of extracellular signal-regulated kinase (ERK) and the inflammatory genes expression, such as interleukin (IL)-6 and tumor necrosis factor alpha (TNF-α)...
July 28, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28673002/igf-1-may-predict-the-severity-and-outcome-of-patients-with-sepsis-and-be-associated-with-microrna-1-level-changes
#9
Liang Xu, Weijun Zhang, Renhua Sun, Jingquan Liu, Jun Hong, Qian Li, Bangchuan Hu, Fangxiao Gong
IGF-1 functions as an anti-oxidative stress molecule and some critical patients with sepsis have a lower level of serum IGF-1. However, the association between IGF-1 and the severity or prognosis of sepsis remains unclear. This study aimed to elucidate the relationship between serum IGF-1 levels and the severity and prognosis of sepsis, and the possible mechanism was analyzed. Clinical characteristics of patients with sepsis were recorded and analyzed. Serum IGF-1 levels and micro (mi)RNA-1 levels were tested using radioimmunoassay and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis, respectively...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28662100/mir-146a-mir-146b-and-mir-155-increase-expression-of-il-6-and-il-8-and-support-hsp10-in-an-in-vitro-sepsis-model
#10
COMPARATIVE STUDY
Dagmar Pfeiffer, Eva Roßmanith, Ingrid Lang, Dieter Falkenhagen
microRNAs (miRNAs) play an essential role in inflammation processes including sepsis. This study aimed to identify miRNAs as candidates for therapies that are involved in the innate immune response and to assess their potential functions in the activation of the endothelium. We stimulated THP-1 monocytes with 10 ng/ml LPS for 4 h and used the supernatant for the stimulation of human umbilical vein endothelial cells (HUVEC) or human pulmonary microvascular endothelial cells (HPMEC) for 16 h. miRNA array analysis (of 1,891 miRNAs) identified a 1...
2017: PloS One
https://www.readbyqxmd.com/read/28655995/microrna-142-3p-and-let-7g-negatively-regulates-augmented-il-6-production-in-neonatal-polymorphonuclear-leukocytes
#11
Hsin-Chun Huang, Hong-Ren Yu, Te-Yao Hsu, I-Lun Chen, Hui-Chen Huang, Jen-Chieh Chang, Kuender D Yang
Neonatal PMN are qualitatively impaired in functions, yet they frequently reveal augmented inflammatory reactions during sepsis. Here, we hypothesized that PMN from newborns produce more IL-6 than those from adults under LPS stimulation, in which transcriptional or posttranscriptional regulation is involved in the altered expression. We found that neonatal PMN produced significantly higher IL-6 mRNA and protein than adult PMN. The higher IL-6 expression was not related to transcriptional but posttranscriptional regulation as the IL-6 expression was affected by the addition of cycloheximide but not actinomycin...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28622229/shields-up-systemic-protection-provided-by-microrna-21-during-sepsis
#12
Cliona Ni Cheallaigh, Frederick J Sheedy
No abstract text is available yet for this article.
July 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28552958/characterization-of-basal-and-lipopolysaccharide-induced-microrna-expression-in-equine-peripheral-blood-mononuclear-cells-using-next-generation-sequencing
#13
Nicholas J Parkinson, Virginia A Buechner-Maxwell, Sharon G Witonsky, R Scott Pleasant, Stephen R Werre, S Ansar Ahmed
The innate immune response to lipopolysaccharide contributes substantially to the morbidity and mortality of gram-negative sepsis. Horses and humans share an exquisite sensitivity to lipopolysaccharide and thus the horse may provide valuable comparative insights into this aspect of the inflammatory response. MicroRNAs, small non-coding RNA molecules acting as post-transcriptional regulators of gene expression, have key roles in toll-like receptor signaling regulation but have not been studied in this context in horses...
2017: PloS One
https://www.readbyqxmd.com/read/28525390/microrna-155-attenuates-late-sepsis-induced-cardiac-dysfunction-through-jnk-and-%C3%AE-arrestin-2
#14
Yu Zhou, Yan Song, Zahir Shaikh, Hui Li, Haiju Zhang, Yi Caudle, Shouhua Zheng, Hui Yan, Dan Hu, Charles Stuart, Deling Yin
Cardiac dysfunction is correlated with detrimental prognosis of sepsis and contributes to a high risk of mortality. After an initial hyperinflammatory reaction, most patients enter a protracted state of immunosuppression (late sepsis) that alters both innate and adaptive immunity. The changes of cardiac function in late sepsis are not yet known. MicroRNA-155 (miR-155) is previously found to play important roles in both regulations of immune activation and cardiac function. In this study, C57BL/6 mice were operated to develop into early and late sepsis phases, and miR-155 mimic was injected through the tail vein 48 h after cecal ligation and puncture (CLP)...
July 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28515178/a-model-specific-role-of-microrna-223-as-a-mediator-of-kidney-injury-during-experimental-sepsis
#15
James F Colbert, Joshay A Ford, Sarah M Haeger, Yimu Yang, Kyrie L Dailey, Kristen C Allison, Viola Neudecker, Christopher M Evans, Vanessa L Richardson, Kelley S Brodsky, Sarah Faubel, Holger K Eltzschig, Eric P Schmidt, Adit A Ginde
Sepsis outcomes are heavily dependent on the development of septic organ injury, but no interventions exist to interrupt or reverse this process. microRNA-223 (miR-223) is known to be involved in both inflammatory gene regulation and host-pathogen interactions key to the pathogenesis of sepsis. The goal of this study was to determine the role of miR-223 as a mediator of septic kidney injury. Using miR-223 knockout mice and multiple models of experimental sepsis, we found that miR-223 differentially influences acute kidney injury (AKI) based on the model used...
August 1, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28497421/decreased-mir-128-and-increased-mir-21-synergistically-cause-podocyte-injury-in-sepsis
#16
Shanshan Wang, Jun Wang, Zengdi Zhang, Hongjun Miao
OBJECTIVE: Glomerular podocytes are injured in sepsis. We studied, in a sepsis patient, whether microRNAs (miRNAs) play a role in the podocyte injury. METHODS: Podocytes were cultured and treated with lipopolysaccharide (LPS). Filtration barrier function of podocyte was analyzed with albumin influx assay. Nephrin level was analyzed with reverse transcription polymerase chain reaction (RT-PCR) and western blot. MiRNAs were detected using miRNAs PCR Array and in situ hybridization...
August 2017: Journal of Nephrology
https://www.readbyqxmd.com/read/28437377/microrna-21-is-required-for-local-and-remote-ischemic-preconditioning-in-multiple-organ-protection-against-sepsis
#17
Ping Jia, Xiaoli Wu, Yan Dai, Jie Teng, Yi Fang, Jiachang Hu, Jianzhou Zou, Mingyu Liang, Xiaoqiang Ding
OBJECTIVE: Sepsis, triggered by microbial infection, is a common and life-threatening systemic illness, often leads to impaired function of vital organs. Ischemic preconditioning induced by transient brief episodes of ischemia is a powerful innate mechanism of organ protection. We have reported that a 15-minute renal ischemic preconditioning substantially attenuated subsequent renal ischemia-reperfusion injury. Here, we investigate whether a brief ischemia and reperfusion in kidney can provide protection at local and remote sites against sepsis-induced organ injury, and whether this protection is microRNA-21 dependent...
July 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28382754/cellular-and-extracellular-mirnas-are-blood-compartment-specific-diagnostic-targets-in-sepsis
#18
Marlene Reithmair, Dominik Buschmann, Melanie Märte, Benedikt Kirchner, Daniel Hagl, Ines Kaufmann, Martina Pfob, Alexander Chouker, Ortrud K Steinlein, Michael W Pfaffl, Gustav Schelling
Septic shock is a common medical condition with a mortality approaching 50% where early diagnosis and treatment are of particular importance for patient survival. Novel biomarkers that serve as prompt indicators of sepsis are urgently needed. High-throughput technologies assessing circulating microRNAs represent an important tool for biomarker identification, but the blood-compartment specificity of these miRNAs has not yet been investigated. We characterized miRNA profiles from serum exosomes, total serum and blood cells (leukocytes, erythrocytes, platelets) of sepsis patients by next-generation sequencing and RT-qPCR (n = 3 × 22) and established differences in miRNA expression between blood compartments...
October 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28288804/mir-146a-potentially-promotes-ivig-mediated-inhibition-of-tlr4-signaling-in-lps-activated-human-monocytes
#19
Lionel Loubaki, Dominique Chabot, Isabelle Paré, Mathieu Drouin, Renée Bazin
IVIg is used as an immunomodulatory agent in inflammatory disorders such as sepsis. IVIg also affects monocyte differentiation and functions, two processes in which microRNAs play a crucial role. Monocytes detect microorganisms through pathogen recognition receptors (PRRs) such as TLR4. MiR-146a has been shown to supress NF-κB and IRF3 activity, two key components of TLR4 signaling. To evaluate whether miR-146a is involved in the anti-inflammatory effects of IVIg, monocytes were treated with LPS or IVIg alone or, alternately, first activated with LPS followed by washing and addition of IVIg...
March 10, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28264607/anti-inflammatory-effects-of-curcumin-are-associated-with-down-regulating-microrna-155-in-lps-treated-macrophages-and-mice
#20
Feiya Ma, Fei Liu, Liang Ding, Ming You, Huimin Yue, Yujie Zhou, Yayi Hou
CONTEXT: The natural polyphenolic compound curcumin has been proved to modulate innate immune responses and possess anti-inflammatory properties. Nevertheless, the mechanism remains poorly understood, particularly regarding curcumin-regulated miRNAs under inflammatory response. OBJECTIVE: This study investigates the role of miRNA-155 in the effects of curcumin on inflammatory response in cell and a mouse model. MATERIALS AND METHODS: The anti-inflammatory activity of curcumin (5, 10 and 15 μM, 2 h) in lipopolysaccharide (LPS, 200 ng/mL)-induced cells were measured by quantitative PCR...
December 2017: Pharmaceutical Biology
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