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MicroRNA AND sepsis

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https://www.readbyqxmd.com/read/29770701/mk2-mediates-macrophage-activation-and-acute-lung-injury-by-regulating-let-7e-mirna
#1
Yaxian Wu, Huiqiong He, Yunhe Ding, Sirui Liu, Depeng Zhang, Jun Wang, Hongchao Jiang, Dan Zhang, Lei Sun, Richard D Ye, Feng Qian
MAPK-activated protein kinase 2 (MK2) plays a critical role in the development of inflammation. However, the modulatory mechanisms in macrophage activation and acute lung injury (ALI) haven't been completely defined. Here, we reported that MK2 deficient mice (MK2-/-) protected against sepsis-induced ALI. In response to lipopolysaccharide (LPS) challenge, MK2-/- mice and myeloid cell-specific MK2 conditional knockout mice (MK2Lyz2-KO) exhibited attenuated inflammatory response, especially producing fewer amounts of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and macrophage inflammatory protein 2 (MIP-2)...
May 17, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29743312/survival-of-mice-with-gastrointestinal-acute-radiation-syndrome-through-control-of-bacterial-translocation
#2
Fujio Suzuki, Bradford D Loucas, Ichiaki Ito, Akira Asai, Sumihiro Suzuki, Makiko Kobayashi
Macrophages (Mϕ) with the M2b phenotype (Pheno2b-Mϕ) in bacterial translocation sites have been described as cells responsible for the increased susceptibility of mice with gastrointestinal acute radiation syndrome to sepsis caused by gut bacteria. In this study, we tried to reduce the mortality of mice exposed to 7-10 Gy of gamma rays by controlling Pheno2b-Mϕ polarization in bacterial translocation sites. MicroRNA-222 was induced in association with gamma irradiation. Pheno2b-Mϕ polarization was promoted and maintained in gamma-irradiated mice through the reduction of a long noncoding RNA growth arrest-specific transcript 5 (a CCL1 gene silencer) influenced by this microRNA...
May 9, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29717063/nuclear-pten-enhances-the-maturation-of-a-microrna-regulon-to-limit-myd88-dependent-susceptibility-to-sepsis
#3
Flavia Sisti, Soujuan Wang, Stephanie L Brandt, Nicole Glosson-Byers, Lindsey D Mayo, Young Min Son, Sarah Sturgeon, Luciano Filgueiras, Sonia Jancar, Hector Wong, Charles S Dela Cruz, Nathaniel Andrews, Jose Carlos Alves-Filho, Fernando Q Cunha, C Henrique Serezani
Sepsis-induced organ damage is caused by systemic inflammatory response syndrome (SIRS), which results in substantial comorbidities. Therefore, it is of medical importance to identify molecular brakes that can be exploited to dampen inflammation and prevent the development of SIRS. We investigated the role of phosphatase and tensin homolog (PTEN) in suppressing SIRS, increasing microbial clearance, and preventing lung damage. Septic patients and mice with sepsis exhibited increased PTEN expression in leukocytes...
May 1, 2018: Science Signaling
https://www.readbyqxmd.com/read/29710503/inhibition-of-microrna-23b-prevents-polymicrobial-sepsis-induced-cardiac-dysfunction-by-modulating-tgif1-and-pten
#4
Haiju Zhang, Yi Caudle, Aamir Shaikh, Baozhen Yao, Deling Yin
Cardiovascular dysfunction is a major complication associated with sepsis induced mortality. Cardiac fibrosis plays a critical role in sepsis induced cardiac dysfunction. The mechanisms of the activation of cardiac fibrosis is unclarified. In this study, we found that microRNA-23b (miR-23b) was up-regulated in heart tissue during cecal ligation and puncture (CLP)-induced sepsis and transfection of miR-23b inhibitor improved survival in late sepsis. Inhibition of miR-23b in the myocardium protected against cardiac output and enhanced left ventricular systolic function...
April 21, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29664004/-advances-in-the-application-of-exosomes-in-sepsis
#5
Dan Wang, Mengna Zhang, Honghong Pei
Exosomes are of great importance in cell communication and information transmission, which have become a hotspot in recent years. Most studies have focused on the transport of microRNA (miRNA) and proteins, that mediated by exosomes, as well as exosomes as a drug delivery carrier of the feasibility. Therefore, exosomes can be an important method of treatment for systemic inflammatory diseases. This article reviews the relevant literature, mainly on the related characteristics of exosomes, its association with clinical disease, and its application in sepsis...
April 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29663998/-microrna-34a-participates-in-lipopolysaccharide-mediated-sepsis-related-renal-function-impairment-via-kruppel-like-factor-4
#6
Qidong Jiang, Changxue Wu, Qiong Zhang
OBJECTIVE: To investigate whether microRNA-34a (miR-34a) participates in lipopolysaccharide (LPS) mediated sepsis related renal function impairment via Kruppel-like factor 4 (KLF4). METHODS: Thirty healthy male Sprague-Dawley (SD) rats, weighing 180-200 g, were randomly divided into two groups: control group and model group, with 15 rats in each group. The SD rats from model group were injected with LPS 7.5 mg/kg to induce sepsis related renal function impairment model, the SD rats from control group were injected with normal saline...
April 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29621481/in-inflamed-intestinal-tissues-and-epithelial-cells-interleukin-22-signaling-increases-expression-of-h19-long-noncoding-rna-which-promotes-mucosal-regeneration
#7
Hua Geng, Heng-Fu Bu, Fangyi Liu, Longtao Wu, Karl Pfeifer, Pauline M Chou, Xiao Wang, Jiaren Sun, Lu Lu, Ashutosh Pandey, Marisa S Bartolomei, Isabelle G De Plaen, Peng Wang, Jindan Yu, Jiaming Qian, Xiao-Di Tan
BACKGROUND & AIMS: Inflammation affects regeneration of the intestinal epithelia; long non-coding RNAs (lncRNAs) regulate cell functions such as proliferation, differentiation, and migration. We investigated the mechanisms by which the lncRNA H19, imprinted maternally expressed transcript (H19) regulates regeneration of intestinal epithelium using cell cultures and mouse models of inflammation. METHODS: We performed RNA-seq transcriptome analyses of intestinal tissues from mice with LPS-induced sepsis to identify lncRNAs associated with inflammation; findings were confirmed by quantitative real-time PCR and in situ hybridization analyses of intestinal tissues from mice with sepsis or dextran sulfate sodium (DSS)-induced mucosal wound healing and patients with ulcerative colitis, compared to healthy individuals (controls)...
April 2, 2018: Gastroenterology
https://www.readbyqxmd.com/read/29599080/exosomes-from-endothelial-progenitor-cells-improve-the-outcome-of-a-murine-model-of-sepsis
#8
Yue Zhou, Pengfei Li, Andrew J Goodwin, James A Cook, Perry V Halushka, Eugene Chang, Hongkuan Fan
Microvascular dysfunction leads to multi-organ failure and mortality in sepsis. Our previous studies demonstrated that administration of exogenous endothelial progenitor cells (EPCs) confers protection in sepsis as evidenced by reduced vascular leakage, improved organ function, and increased survival. We hypothesize that EPCs protect the microvasculature through the exosomes-mediated transfer of microRNAs (miRNAs). Mice were rendered septic by cecal ligation and puncture (CLP), and EPC exosomes were administered intravenously at 4 hr after CLP...
February 27, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29568906/mir-124-mcp-1-signaling-pathway-modulates-the-protective-effect-of-itraconazole-on-acute-kidney-injury-in-a-mouse-model-of-disseminated-candidiasis
#9
Xiao-Yue Li, Yu-Qi Zhang, Gang Xu, Shao-Hong Li, Heng Li
Previous studies have indicated that monocyte chemoattractant protein-1 (MCP‑1), also referred to as C‑C motif chemokine ligand 2, has a significant role in the pathogenesis of sepsis, however, how microRNAs (miRs) contribute to this process remains to be fully elucidated. In the present study, using a mouse model of disseminated candidiasis, the renoprotective effect of itraconazole (ITR) and adenovirus‑delivered miR‑124 was investigated. The mice were treated with ITR (50 mg/kg) or transfected with miR‑124 mimics via tail‑vein injection 7 days prior to Candida albicans infection...
March 13, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29565737/endothelial-micrornas-regulating-the-nf-%C3%AE%C2%BAb-pathway-and-cell-adhesion-molecules-during-inflammation
#10
Liang Zhong, Martin J Simard, Jacques Huot
The surface of endothelial cells is covered with cell adhesion molecules, including E-selectin, intercellular adhesion molecule 1 (ICAM-1), and vascular cell adhesion molecule 1 (VCAM- 1) , that mediate the adhesion and extravasation of leukocytes and play pivotal roles in inflammatory response. microRNAs (miRNAs) regulate the expression of these important cell adhesion molecules through two distinct major mechanisms, namely via modulating the proinflammatory NF-κB pathway, which controls their transcription, and via directly targeting them...
March 22, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29560499/-epigenetics-important-aspects-for-anesthesiologists-pain-and-intensive-care-physicians
#11
REVIEW
A-K Reinhold, E Jentho, S T Schäfer, M Bauer, H L Rittner
Epigenetics, i.e. an altered reading of the genome without altering the genes themselves is a growing scientific field. A distinction is made between changes in the DNA by modification of the histones and non-coding RNA that alter the messenger (m)RNAs. Epigenetic modifications can be triggered by personal circumstances or other external factors and therefore influence the occurrence of diseases. Epigenetics are therefore of particular interest to anesthesiologists, pain specialists and intensive care physicians, as anesthetic drugs may have a long-term influence on protein transcription leading for example to alterations in neurocognition after anesthesia, chronification of postoperative pain and immune response in sepsis...
March 20, 2018: Der Anaesthesist
https://www.readbyqxmd.com/read/29540208/exosomes-from-patients-with-septic-shock-convey-mirnas-related-to-inflammation-and-cell-cycle-regulation-new-signaling-pathways-in-sepsis
#12
Juliana Monte Real, Ludmila Rodrigues Pinto Ferreira, Gustavo Henrique Esteves, Fernanda Christtanini Koyama, Marcos Vinícius Salles Dias, João Evangelista Bezerra-Neto, Edécio Cunha-Neto, Flavia Ribeiro Machado, Reinaldo Salomão, Luciano Cesar Pontes Azevedo
BACKGROUND: Exosomes isolated from plasma of patients with sepsis may induce vascular apoptosis and myocardial dysfunction by mechanisms related to inflammation and oxidative stress. Despite previous studies demonstrating that these vesicles contain genetic material related to cellular communication, their molecular cargo during sepsis is relatively unknown. In this study, we evaluated the presence of microRNAs (miRNAs) and messenger RNAs (mRNAs) related to inflammatory response and redox metabolism in exosomes of patients with septic shock...
March 15, 2018: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/29512785/identification-of-potential-genes-and-mirnas-associated-with-sepsis-based-on-microarray-analysis
#13
Yin Li, Fengxia Zhang, Yan Cong, Yun Zhao
Sepsis is a type of systemic inflammatory response syndrome caused by infection. The present study aimed to examine key genes and microRNAs (miRNAs) involved in the pathogenesis of sepsis. The GSE13205 microarray dataset, downloaded from the Gene Expression Omnibus was analyzed using bioinformatics tools, and included muscle biopsy specimens of 13 patients with sepsis and eight healthy controls. The differentially expressed genes (DEGs) in samples from patients with sepsis were identified using the Linear Models for Microarray package in R language...
May 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29506272/inhibition-of-microrna-23b-attenuates-immunosuppression-in-late-sepsis-through-nik-traf1-and-xiap
#14
Haiju Zhang, Hui Li, Aamir Shaikh, Yi Caudle, Baozhen Yao, Deling Yin
Background: microRNA-23b (miR-23b) is a multiple functional miRNA. We hypothesize that miR-23b plays a role in the pathogenesis of sepsis. Our study investigated the effect of miR-23b on sepsis induced immunosuppression. Methods: Mice were treated with miR-23b inhibitors by tail vein injection 2 days after cecal ligation puncture (CLP)-induced sepsis. Apoptosis in spleens and apoptotic signals were detected, meanwhile survival was monitored. T cell immunoreactivities were examined in late sepsis...
March 1, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29442046/microrna-146-regulates-the-inflammatory-cytokines-expression-in-vascular-endothelial-cells-during-sepsis
#15
Ning Gao, Lei Dong
AIMS: The purpose of this study was to investigate the functional role of microRNA (miR)-146 in sepsis, as well as the underlying mechanism. METHODS: Human vascular endothelial cell line EA. hy926 cells were treated with lipopolysaccharide (LPS) and/or transfected with miR-146 mimics, inhibitor, and their corresponding controls. Expression of miR-146 was then analyzed after treatment and/or transfection, as well as the expression of inflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-6, intercellular adhesion molecule (ICAM)-1, and E-selectin, and nuclear factor kappa B (NF-κB) binding activity...
November 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29434809/microrna-30a-3p-overexpression-improves-sepsis-induced-cell-apoptosis-in-vitro-and-in-vivo-via-the-pten-pi3k-akt-signaling-pathway
#16
Shuying Yang, Yongqiang Wang, Hongmei Gao, Bing Wang
The aim of the present study was to explain the mechanism of miR-30a-3p overexpression in sepsis-induced cell apoptosis in vitro and in vivo . For the in vitro cell experiments, H9c2 cells were divided into three groups, including the untreated normal control (NC), lipopolysaccharide (LPS)-treated and miRNA (treated with LPS and transfection with miRNA-30a-3p) groups. The cell proliferation and apoptosis were evaluated by MTT assay and flow cytometry, respectively. The relative protein expression levels were measured by western blot assay...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29245971/systematic-analysis-of-the-expression-profile-of-non-coding-rnas-involved-in-ischemia-reperfusion-induced-acute-kidney-injury-in-mice-using-rna-sequencing
#17
Jun Zhou, Hongtao Chen, Youling Fan
Acute kidney injury (AKI) is a common and serious disease characterized by a rapid decline in renal function and has an unacceptably high mortality rate with no effective treatment beyond supportive care. AKI can be induced by many factors such as ischemia/reperfusion (IR), sepsis, and drug-induced nephrotoxicity. However, the molecular mechanisms of AKI are poorly understood. A non-coding RNA (ncRNA) is a RNA molecule that is not translated into a protein. NcRNAs play multiple roles in cellular processes, and mutations or imbalances of these molecules within the body can cause a variety of diseases...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29237456/identification-of-potential-transcriptomic-markers-in-developing-pediatric-sepsis-a-weighted-gene-co-expression-network-analysis-and-a-case-control-validation-study
#18
Yiping Li, Yanhong Li, Zhenjiang Bai, Jian Pan, Jian Wang, Fang Fang
BACKGROUND: Sepsis represents a complex disease with the dysregulated inflammatory response and high mortality rate. The goal of this study was to identify potential transcriptomic markers in developing pediatric sepsis by a co-expression module analysis of the transcriptomic dataset. METHODS: Using the R software and Bioconductor packages, we performed a weighted gene co-expression network analysis to identify co-expression modules significantly associated with pediatric sepsis...
December 13, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29207683/non-coding-rna-a-potential-biomarker-and-therapeutic-target-for-sepsis
#19
REVIEW
Tie-Ning Zhang, Da Li, Jing Xia, Qi-Jun Wu, Ri Wen, Ni Yang, Chun-Feng Liu
Sepsis, a syndrome of physiologic, pathologic, and biochemical abnormalities caused by an altered systemic host response to infection, has become the main cause of death among patients admitted to the intensive care units. Recently, genome-wide expression analysis revealed that over 80% of the essential genetic elements were altered in critically ill patients. Notably, non-coding RNAs, including microRNAs, long non-coding RNAs and circular RNAs, have been proven to play essential roles in innate immunity, mitochondrial dysfunction and organ dysfunction...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29161706/endotoxin-effects-on-cardiac-and-renal-functions-and-cardiorenal-syndromes
#20
Grazia Maria Virzì, Anna Clementi, Alessandra Brocca, Claudio Ronco
Gram-negative sepsis is a major cause of morbidity and mortality in critical ill patients. Recent findings in molecular biology and in signaling pathways have enhanced our understanding of its pathogenesis and opened up opportunities of innovative therapeutic approaches. Endotoxin plays a pivotal role in the pathogenesis of multi-organ dysfunction in the setting of gram-negative sepsis. Indeed, heart and kidney impairments seem to be induced by the release of circulating pro-inflammatory and pro-apoptotic mediators triggered by endotoxin interaction with immune cells...
2017: Blood Purification
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