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https://www.readbyqxmd.com/read/28525658/genotyping-of-snps-associated-with-meat-tenderness-comparison-of-two-pcr-based-methods
#1
L E López-Rojas, L Patiño-Cadavid, A López-Herrera, J J Echeverri-Zuluaga
Single nucleotide polymorphisms (SNPs) carried in calpain (CAPN1), calpastatin (CAST), and leptin (LEP) genes are associated with meat tenderness. Due to the economic importance of this meat quality attribute, the development of fast, reliable, and affordable methods to identify bovine carriers of favorable alleles is of great importance for genetic improvement. Currently, PCR-RFLP is accepted as the standard gold method for genotyping SNPs associated with meat tenderness. But these SNPs can be detected by other techniques as high-resolution melting (HRM) analysis - a post-PCR method - that offers several advantages and has great application potential in the meat industry...
May 18, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28523673/the-function-of-language-in-parent-infant-psychotherapy
#2
Björn Salomonsson
Parent-infant psychotherapy, a rather new field in psychoanalysis, raises questions of how to conceptualize the clinical process. Previous publications have used semiotic concepts to account for the therapist's non-verbal communication and investigated the countertransference, including what the baby might grasp of its variations. The present paper focuses on another argument for using verbal interventions to a baby in therapy; they present him with a symbolic order that differs from that of the parent. The qualitative difference between the parent's and the analyst's address is conceptualized by Dolto's term parler vrai...
May 19, 2017: International Journal of Psycho-analysis
https://www.readbyqxmd.com/read/28523579/primary-care-and-hepatology-provider-perceived-barriers-to-and-facilitators-of-hepatitis-c-treatment-candidacy-and-adherence
#3
Shari S Rogal, Rory McCarthy, Andrea Reid, Keri L Rodriguez, Linda Calgaro, Krupa Patel, Molly Daley, Naudia L Jonassaint, Susan L Zickmund
BACKGROUND: Provider perceptions regarding barriers to and facilitators of hepatitis C (HCV) treatment initiation and adherence have not been fully evaluated in the interferon-free treatment era. New treatments have provided opportunities for non-specialists to treat HCV, underscoring the importance of understanding primary care provider (PCP) and specialist perspectives. METHODS: Based on qualitative sampling principles, 12 PCPs and 12 hepatology providers (HPs) from the VA Pittsburgh Healthcare System completed audio-recorded semi-structured interviews...
May 18, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28520796/a-hierarchical-model-for-estimating-the-spatial-distribution-and-abundance-of-animals-detected-by-continuous-time-recorders
#4
Robert M Dorazio, K Ullas Karanth
MOTIVATION: Several spatial capture-recapture (SCR) models have been developed to estimate animal abundance by analyzing the detections of individuals in a spatial array of traps. Most of these models do not use the actual dates and times of detection, even though this information is readily available when using continuous-time recorders, such as microphones or motion-activated cameras. Instead most SCR models either partition the period of trap operation into a set of subjectively chosen discrete intervals and ignore multiple detections of the same individual within each interval, or they simply use the frequency of detections during the period of trap operation and ignore the observed times of detection...
2017: PloS One
https://www.readbyqxmd.com/read/28500214/efficacy-of-the-telemedical-lifestyle-intervention-program-telipro-in-advanced-stages-of-type-2-diabetes-a-randomized-controlled-trial
#5
Kerstin Kempf, Bernd Altpeter, Janine Berger, Oliver Reuß, Matthias Fuchs, Michael Schneider, Babette Gärtner, Katja Niedermeier, Stephan Martin
OBJECTIVE: Lifestyle interventions are the foundation of treatment in newly diagnosed type 2 diabetes. However, their therapeutic potential in advanced disease stages is unknown. We evaluated the efficacy of the Telemedical Lifestyle intervention Program (TeLiPro) in improving metabolic control in advanced-stage type 2 diabetes. RESEARCH DESIGN AND METHODS: In this single-blind, active comparator, intervention study, patients with type 2 diabetes (with glycated hemoglobin [HbA1c] ≥7...
May 12, 2017: Diabetes Care
https://www.readbyqxmd.com/read/28499599/gas-chromatography-mass-spectrometry-data-processing-made-easy
#6
Lea G Johnsen, Peter B Skou, Bekzod Khakimov, Rasmus Bro
Evaluation of GC-MS data may be challenging due to the high complexity of data including overlapped, embedded, retention time shifted and low S/N ratio peaks. In this work, we demonstrate a new approach, PARAFAC2 based Deconvolution and Identification System (PARADISe), for processing raw GC-MS data. PARADISe is a computer platform independent freely available software incorporating a number of newly developed algorithms in a coherent framework. It offers a solution for analysts dealing with complex chromatographic data...
April 27, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28498831/dynamics-of-analyst-forecasts-and-emergence-of-complexity-role-of-information-disparity
#7
Chansoo Kim, Daniel S Kim, Kwangwon Ahn, M Y Choi
We report complex phenomena arising among financial analysts, who gather information and generate investment advice, and elucidate them with the help of a theoretical model. Understanding how analysts form their forecasts is important in better understanding the financial market. Carrying out big-data analysis of the analyst forecast data from I/B/E/S for nearly thirty years, we find skew distributions as evidence for emergence of complexity, and show how information asymmetry or disparity affects financial analysts' forming their forecasts...
2017: PloS One
https://www.readbyqxmd.com/read/28489597/the-prevalence-of-depression-in-rheumatoid-arthritis-in-china-a-systematic-review
#8
Xin Fu, Zhi-Jun Li, Chun-Jun Yang, Liangshu Feng, Lemeng Sun, Yang Yao, Yu-Ting Huang
This systematic review is to explore the prevalence of depression in patients with rheumatoid arthritis (RA) in China. Articles of prevalence rates for depression in adult RA patients published before October 2015 were identified from PubMed, Embase, The Cochrane Library, CNKI, CBM, VIP, and Wanfang database and other internet databases. Relevant journals and the recommendations of expert panels were also searched manually. Two independent reviewers searched and assessed the literature. Therelevant data were applied with Meta-Analyst 3...
April 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28486201/novel-spectrofluorimetric-method-for-boldine-alkaloid-determination-in-herbal-drugs-and-phytopharmaceuticals
#9
Cecilia M Peralta, Cecilia Henestrosa, Raúl A Gil, Liliana P Fernández, Gimena Acosta
A new green on-line method for Boldine determination (BOL) in herbal drugs and phytopharmaceuticals, using its native fluorescence in acid media (λex=282nm; λem=373nm) has been developed. The presented methodology involves for the first time, a flow injection (FI) strategy using a mini-column of multiwalled carbon nanotubes as retention agent coupled with molecular fluorescence. Different parameters influence as sample pH and flow rate, eluent flow rate and composition; on BOL sensitivity and elution time was investigated by multifactorial techniques...
April 21, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28483071/efficacy-and-tolerability-of-vilazodone-for-the-acute-treatment-of-generalized-anxiety-disorder-a-meta-analysis
#10
Nicholas Zareifopoulos, Irene Dylja
PURPOSE: A systematic review and meta-analysis of all relevant randomized controlled trials was conducted to evaluate the safety and efficacy of vilazodone in the acute treatment of generalized anxiety disorder (GAD). METHODS: The literature was searched through all relevant databases in order to identify clinical trials on the use of vilazodone in the treatment of GAD. Once the trials were identified, data was extracted and analyzed using Revman5.3 and open meta-analyst...
April 2017: Asian Journal of Psychiatry
https://www.readbyqxmd.com/read/28481820/a-prenatal-coparenting-intervention-with-unmarried-father-mother-dyads-fidelity-of-intervention-delivery-by-male-female-community-mentor-teams
#11
Selin Salman-Engin, Tara Little, Vikki Gaskin-Butler, James P McHale
BACKGROUND: Most prenatal preventive interventions for unmarried mothers do not integrate fathers or help the parents plan for the development of a functional coparenting alliance after the baby's arrival. Furthermore, properly trained professionals have only rarely examined the fidelity of these interventions. PURPOSE: This report examines whether experienced community interventionists (home visitors, health educators, fatherhood service personnel) with no formal couples' therapy training are capable of pairing together to deliver with adequate fidelity a manualized dyadic intervention designed for expectant unmarried mothers and fathers...
June 2017: Journal of Nursing Research: JNR
https://www.readbyqxmd.com/read/28479281/explaining-match-outcome-and-ladder-position-in-the-national-rugby-league-using-team-performance-indicators
#12
Carl T Woods, Wade Sinclair, Sam Robertson
OBJECTIVES: To examine the extent at which match outcome and ladder position could be explained using team performance indicators in the National Rugby League (NRL). METHODS: The dataset consisted of 13 performance indicators acquired from each NRL team across the 2016 season (n=376 observations). Data was sorted according to apriori match outcome (win/loss) and ladder position (one to 16). Given the binary and categorical nature of the response variables, two analysis approaches were used; a conditional interference classification tree and ordinal regression...
April 21, 2017: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/28472756/development-and-validation-of-innoquant-%C3%A2-hy-a-system-for-quantitation-and-quality-assessment-of-total-human-and-male-dna-using-high-copy-targets
#13
Andrew Loftus, Gina Murphy, Hiromi Brown, Anne Montgomery, Jonathan Tabak, James Baus, Marion Carroll, André Green, Suresh Sikka, Sudhir Sinha
The development and validation of InnoQuant(®) HY, a real-time PCR system containing four DNA targets-two RE autosomal targets of different sizes, male specific targets, and an internal positive control target-are described herein. The ratio of the two autosomal targets provides a Degradation Index, or a quantitative value of a sample's degradation state. The male specific targets are multi-copy targets located on the Y chromosome, which provides information about a sample's male DNA composition. The experimental results demonstrate InnoQuant HY as a robust qPCR method producing accurate DNA quantitation results even at low dynamic ranges, with reproducibility among population groups...
April 17, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28468952/reliability-of-an-automatic-classifier-for-brain-enlarged-perivascular-spaces-burden-and-comparison-with-human-performance
#14
Victor Gonzalez-Castro, Maria Del Carmen Valdes-Hernandez, Francesca Chappell, Paul A Armitage, Stephen Makin, Joanna Wardlaw
In the brain, enlarged perivascular spaces (PVS) relate to cerebral small vessel disease, poor cognition, inflammation and hypertension. We propose a fully automatic scheme that uses a support vector machine(SVM) to classify the burden of PVS in the basal ganglia(BG) region as low or high. We assess the performance of three different types of descriptors extracted from the BG region in T2-weighted MRI images: 1)statistics obtained from Wavelet transform's coefficients, 2)local binary patterns and 3)bag of visual words (BoW)-based descriptors characterising local keypoints obtained from a dense grid with the scale-invariant feature transform characteristics...
May 3, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28467023/the-discounting-model-selector-statistical-software-for-delay-discounting-applications
#15
Shawn P Gilroy, Christopher T Franck, Donald A Hantula
Original, open-source computer software was developed and validated against established delay discounting methods in the literature. The software executed approximate Bayesian model selection methods from user-supplied temporal discounting data and computed the effective delay 50 (ED50) from the best performing model. Software was custom-designed to enable behavior analysts to conveniently apply recent statistical methods to temporal discounting data with the aid of a graphical user interface (GUI). The results of independent validation of the approximate Bayesian model selection methods indicated that the program provided results identical to that of the original source paper and its methods...
May 3, 2017: Journal of the Experimental Analysis of Behavior
https://www.readbyqxmd.com/read/28466493/the-reliability-of-swipe-wipe-classification-and-directionality-determination-methods-in-bloodstain-pattern-analysis
#16
Sita K Y Yuen, Michael C Taylor, Glynn Owens, Douglas A Elliot
Swipe and wipe are types of transfer patterns commonly encountered at bloodied scenes. So far, there have been little published data on the reliability of evaluating these patterns. In this study, 36 bloodstain patterns were randomly assigned to 12 analysts in three separate trials, and the analysts were required to evaluate the pattern type and directionality. The results revealed that correct classifications of patterns type were problematic, with an overall error rate of 32%. Wipes created from wet bloodstains were the most difficult to identify due to the absence of signs of alteration...
May 3, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28464865/empathy-in-psychoanalysis-and-medical-education-what-can-we-learn-from-each-other
#17
Henriette Löffler-Stastka, Felicitas Datz, Karoline Parth, Ingrid Preusche, Xenia Bukowski, Charles Seidman
BACKGROUND: Several research areas, including medical education (ME), focus on empathy as an important topic in interpersonal relationships. This focus is central to the use of communication skills related to empathy and even more crucial to provide information in a way that makes patients feel more involved in the treatment process. Psychoanalysis (PA) provides its initial concept of empathy based on affective aspects including findings from neuroscience and brain research. Enhancing cooperation between ME and PA can help to integrate both aspects of empathy into a longitudinal training program...
May 2, 2017: BMC Medical Education
https://www.readbyqxmd.com/read/28464790/automated-multigroup-outlier-identification-in-molecular-high-throughput-data-using-bagplots-and-gemplots
#18
Jochen Kruppa, Klaus Jung
BACKGROUND: Analyses of molecular high-throughput data often lack in robustness, i.e. results are very sensitive to the addition or removal of a single observation. Therefore, the identification of extreme observations is an important step of quality control before doing further data analysis. Standard outlier detection methods for univariate data are however not applicable, since the considered data are high-dimensional, i.e. multiple hundreds or thousands of features are observed in small samples...
May 2, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28460316/a-comprehensive-regional-analysis-of-genome-wide-expression-profiles-for-major-depressive-disorder
#19
Diego A Forero, Gina P Guio-Vega, Yeimy González-Giraldo
BACKGROUND: Major depressive disorder (MDD) is a global health challenge. In recent years, a large number of genome-wide expression studies (GWES) have been carried out to identify the transcriptomic profiles for MDD. The objective of this work was to carry out a comprehensive meta-analysis of available GWES for MDD. METHODS: GWES for MDD with available raw data were searched in NCBI GEO, Array Express and Stanley databases. Raw GWES data were preprocessed and normalized and meta-analytical procedures were carried out with the Network Analyst program...
April 26, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/28459690/indexed-points-parallel-coordinates-visualization-of-multivariate-correlations
#20
Liang Zhou, Daniel Weiskopf
We address the problem of visualizing multivariate correlations in parallel coordinates. We focus on multivariate correlation in the form of linear relationships between multiple variables. Traditional parallel coordinates are well prepared to show negative correlations between two attributes by distinct visual patterns. However, it is difficult to recognize positive correlations in parallel coordinates. Furthermore, there is no support to highlight multivariate correlations in parallel coordinates. In this paper, we exploit the indexed point representation of p -flats (planes in multidimensional data) to visualize local multivariate correlations in parallel coordinates...
April 25, 2017: IEEE Transactions on Visualization and Computer Graphics
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