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https://www.readbyqxmd.com/read/28750498/emerging-clinical-issues-and-multivariate-analyses-in-pet-investigations
#1
Javier Arbizu, Alessandro Giuliani, Jaime G Perez-Larraya, Mario Riverol, Cathrine Jonsson, Berta García-García, Maribel Morales, Laura Imaz, Marco Pagani
PET using 18F-2-fluoro-2-deoxy-D-glucose (FDG-PET) has been gradually introduced in the diagnostic clinical criteria of the most prevalent neurodegenerative diseases. Moreover, an increasing amount of literature have shown that the information provided by FDG-PET enhances the sensitivity of standard imaging biomarkers in less frequent disorders in which an early differential diagnosis can be of paramount relevance for patient management and outcome. Therefore emerging uses of FDG-PET may be important in prion diseases, autoimmune encephalitis and amyotrophic lateral sclerosis...
July 27, 2017: Quarterly Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/28750075/cbirtox-is-a-selective-antigen-specific-agonist-of-the-treg-iga-microbiota-homeostatic-pathway
#2
Katie L Alexander, Jannet Katz, Charles O Elson
Cultivating an environment of mutualism between host cells and the microbiota is vital, and dysregulation of this relationship is associated with multiple immune disorders including metabolic and skin diseases, asthma, allergy, and Inflammatory Bowel Disease (IBD). One prominent mechanism for maintaining homeostasis is the protective regulatory T cell (Treg)- Immunoglobulin A (IgA) pathway toward microbiota antigens, in which Tregs maintain homeostasis and provide critical survival factors to IgA+ B cells. In order to amplify the Treg-IgA pathway, we have generated a fusion protein, CBirTox, comprised of a portion of the carboxy terminus of CBir1, a microbiota flagellin, genetically coupled to Cholera Toxin B subunit (CTB) via the A2 linker of CT...
2017: PloS One
https://www.readbyqxmd.com/read/28750056/fatty-binding-protein-and-galectin-of-baylisascaris-schroederi-prokaryotic-expression-and-preliminary-evaluation-of-serodiagnostic-potential
#3
Ying Sun, Yu Li, Yiran Wu, Lang Xiong, Caiwu Li, Chengdong Wang, Desheng Li, Jingchao Lan, Zhihe Zhang, Bo Jing, Xiaobing Gu, Yue Xie, Weimin Lai, Xuerong Peng, Guangyou Yang
Baylisascaris schroederi is a common parasite of captive giant pandas. The diagnosis of this ascariasis is normally carried out by a sedimentation-floatation method or PCR to detect eggs in feces, but neither method is suitable for early diagnosis. Fatty acid-binding protein (FABP) and galectin (GAL) exist in various animals and participate in important biology of parasites. Because of their good immunogenicity, they are seen as potential antigens for the diagnosis of parasitic diseases. In this study, we cloned and expressed recombinant FABP and GAL from B...
2017: PloS One
https://www.readbyqxmd.com/read/28749929/chemoenzymatic-synthesis-of-glycoengineered-igg-antibodies-and-glycosite-specific-antibody-drug-conjugates
#4
Feng Tang, Lai-Xi Wang, Wei Huang
Glycoengineered therapeutic antibodies and glycosite-specific antibody-drug conjugates (gsADCs) have generated great interest among researchers because of their therapeutic potential. Endoglycosidase-catalyzed in vitro glycoengineering technology is a powerful tool for IgG Fc (fragment cystallizable) N-glycosylation remodeling. In this protocol, native heterogeneously glycosylated IgG N-glycans are first deglycosylated with a wild-type endoglycosidase. Next, a homogeneous N-glycan substrate, presynthesized as described here, is attached to the remaining N-acetylglucosamine (GlcNAc) of IgG, using a mutant endoglycosidase (also called endoglycosynthase) that lacks hydrolytic activity but possesses transglycosylation activity for glycoengineering...
August 2017: Nature Protocols
https://www.readbyqxmd.com/read/28749414/adjuvantation-of-pulmonary-administered-influenza-vaccine-with-gpi-0100-primarily-stimulates-antibody-production-and-memory-b-cell-proliferation
#5
Harshad P Patil, José Herrera Rodriguez, Jacqueline de Vries-Idema, Tjarko Meijerhof, Henderik W Frijlink, Wouter L J Hinrichs, Anke Huckriede
Adjuvants are key components in vaccines, they help in reducing the required antigen dose but also modulate the phenotype of the induced immune response. We previously showed that GPI-0100, a saponin-derived adjuvant, enhances antigen-specific mucosal and systemic antibody responses to influenza subunit and whole inactivated influenza virus (WIV) vaccine administered via the pulmonary route. However, the impact of the GPI-0100 dose on immune stimulation and the immune mechanisms stimulated by GPI-0100 along with antigen are poorly understood...
July 27, 2017: Vaccines
https://www.readbyqxmd.com/read/28748722/gallium-68-prostate-specific-membrane-antigen-positron-emission-tomography-computed-tomography-for-staging-of-high-risk-prostate-cancer
#6
Lars J Petersen, Helle D Zacho
No abstract text is available yet for this article.
July 27, 2017: Scandinavian Journal of Urology
https://www.readbyqxmd.com/read/28748460/principles-and-methods-of-immunohistochemistry
#7
José A Ramos-Vara
Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in the characterization of normal and pathologic tissue and the reagents used. Pretreatments such as blocking steps for endogenous activities and antigen retrieval are included. Standard procedures on formalin-fixed, paraffin-embedded tissues as well as method standardization for new antibodies and troubleshooting are emphasized...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28748396/disagreement-between-the-results-from-three-commercial-tests-for-the-detection-of-borrelia-specific-serum-antibodies-in-the-netherlands-associated-with-antibiotic-treatment-for-lyme-borreliosis-a-retrospective-study
#8
T van Gorkom, K Kremer, W Voet, D W Notermans, B J M Vlaminckx, S U C Sankatsing, S F T Thijsen
The diagnosis of Lyme borreliosis is challenging because of the often non-specific symptoms and persisting antibodies after infection. We investigated the diagnostic characteristics of two enzyme-linked immunosorbent assays (ELISAs) and an immunoblot for the detection of Borrelia-specific serum antibodies using different test strategies in individuals with and without antibiotic treatment for Lyme borreliosis. This retrospective study included healthy individuals, patients with active Lyme neuroborreliosis and patients treated for Lyme neuroborreliosis...
July 26, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28748318/surgical-outcome-of-wide-wedge-resection-in-poor-risk-patients-with-clinical-n0-non-small-cell-lung-cancer
#9
Hiroaki Tsunezuka, Daishiro Kato, Satoru Okada, Tatsuo Furuya, Junichi Shimada, Masayoshi Inoue
OBJECTIVE: Sublobar resection is practically indicated in poor-risk patients compromised cardiopulmonary reserve or for small ground-glass opacity-dominant adenocarcinomas. The aim of this study was to clarify the surgical outcomes of wide wedge resection in poor-risk patients with clinical-N0 non-small cell lung cancers. METHODS: Sixty-two poor-risk patients, who underwent wedge resection for clinical-N0 lung cancer, were retrospectively reviewed. We defined the ratio of the maximum diameter of the consolidation to the maximum tumour diameter as the consolidation-to-tumour ratio using high-resolution computed tomography...
July 26, 2017: General Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28748206/the-complementarity-determining-region-sequences-in-igy-antivenom-hypervariable-regions
#10
David Gitirana da Rocha, Jorge Hernandez Fernandez, Claudia Maria Costa de Almeida, Claudia Letícia da Silva, Fabio Carlos Magnoli, Osmair Élder da Silva, Wilmar Dias da Silva
The data presented in this article are related to the research article entitled "Development of IgY antibodies against anti-snake toxins endowed with highly lethal neutralizing activity" (da Rocha et al., 2017) [1]. Complementarity-determining region (CDR) sequences are variable antibody (Ab) sequences that respond with specificity, duration and strength to identify and bind to antigen (Ag) epitopes. B lymphocytes isolated from hens immunized with Bitis arietans (Ba) and anti-Crotalus durissus terrificus (Cdt) venoms and expressing high specificity, affinity and toxicity neutralizing antibody titers were used as DNA sources...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28748166/assessment-of-immunomodulatory-activity-of-aqueous-extract-of-calamus-rotang
#11
Amit Gupta, Sushama Ravindra Chaphalkar
OBJECTIVE: There are a number of medicinal plant products which has been used to treat various immunological diseases. Out of these medicinal plants, Calamus rotang has shown several medicinal properties including anti-viral, anti-diabetic, anti-inflammatory effects. Normally, the roots of C. rotang are used in various ailments to cure piles, burning sensation, cough, leprosy and bleeding disorder and also it was used in the treatment of inflammation. In the present study, our group were investigated the immunomodulatory activity of aqueous extract of C...
May 2017: Avicenna Journal of Phytomedicine
https://www.readbyqxmd.com/read/28747996/triptorelin-for-the-relief-of-lower-urinary-tract-symptoms-in-men-with-advanced-prostate-cancer-results-of-a-prospective-observational-grouped-analysis-study
#12
Thierry Gil, Fouad Aoun, Patrick Cabri, Valérie Perrot, Roland van Velthoven
BACKGROUND: Lower urinary tract symptoms (LUTSs) may develop in men with prostate cancer (PCa) and can impact quality of life (QoL). Gonadotropin-releasing hormone (GnRH) agonists as androgen deprivation therapy are standard treatment for PCa, however, data are limited on their effects on LUTSs. A grouped analysis of national observational, non-interventional studies initiated in clinical practice was performed to assess the effectiveness of triptorelin in reducing moderate or severe LUTSs, measured using the International Prostate Symptom Score (IPSS) in men with advanced or metastatic PCa...
July 2017: Therapeutic Advances in Urology
https://www.readbyqxmd.com/read/28747941/the-prognostic-value-of-carcinoembryonic-antigen-levels-in-blood-and-intraoperative-pleural-lavage-fluid-in-non-small-cell-lung-cancer
#13
Tevfik Ilker Akcam, Ufuk Cagirici, Ayse Gul Ergonul, Ali Ozdil, Onder Kavurmaci, Kutsal Turhan, Alpaslan Cakan, Burcu Barutcuoglu
INTRODUCTION: There is no specific marker for lung cancer, but, in some lung cancer types, carcinoembryonic antigen (CEA) can reach high levels in the blood and pleural fluid. AIM: This study investigated the relationship of CEA levels in blood (CEAB) and intraoperative pleural lavage fluid (CEAP) in non-small-cell lung cancer (NSCLC) with the type, stage, and extent of lung cancer. MATERIAL AND METHODS: A total of 50 patients, who underwent surgery at our clinic due to NSCLC (group I) or benign lung pathology (group II), were assessed...
June 2017: Kardiochirurgia i Torakochirurgia Polska, Polish Journal of Cardio-Thoracic Surgery
https://www.readbyqxmd.com/read/28747911/f1-domain-of-the-leishmania-leishmania-donovani-nucleoside-hydrolase-promotes-a-th1-response-in-leishmania-leishmania-infantum-cured-patients-and-in-asymptomatic-individuals-living-in-an-endemic-area-of-leishmaniasis
#14
Eugenia Carrillo, Laura Fernandez, Ana Victoria Ibarra-Meneses, Micheli L B Santos, Dirlei Nico, Paula M de Luca, Cristiane Bani Correa, Roque Pacheco de Almeida, Javier Moreno, Clarisa B Palatnik-de-Sousa
The Leishmania (Leishmania) donovani nucleoside hydrolase NH36 is the main antigen of the Leishmune(®) vaccine and one of the promising candidates for vaccination against visceral leishmaniasis. The antigenicity of the N-terminal (F1), the central (F2), or the C-terminal recombinant domain (F3) of NH36 was evaluated using peripheral blood mononuclear cells (PBMC) from individuals infected with L. (L.) infantum from an endemic area of visceral leishmaniasis of Spain. Both NH36 and F1 domains significantly increased the PBMC proliferation stimulation index of cured patients and infected asymptomatic individuals compared to healthy controls...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28747910/natural-killer-cell-based-cancer-immunotherapies-from-immune-evasion-to-promising-targeted-cellular-therapies
#15
Erhard Hofer, Ulrike Koehl
Immunotherapies based on natural killer (NK) cells are among the most promising therapies under development for the treatment of so far incurable forms of leukemia and other types of cancer. The importance of NK cells for the control of viral infections and cancer is supported among others by the findings that viruses and tumors use a multitude of mechanisms to subvert and evade the NK cell system. Infections and malignant diseases can further lead to the shaping of NK cell populations with altered reactivity...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28747573/the-influence-of-adjuvant-on-ureb-protection-against-helicobacter-pylori-through-the-diversity-of-cd4-t-cell-epitope-repertoire
#16
Bin Li, Hanmei Yuan, Li Chen, Heqiang Sun, Jian Hu, Shanshan Wei, Zhuo Zhao, Quanming Zou, Chao Wu
Adjuvants are widely used to enhance the effects of vaccines against pathogen infections. Interestingly, different adjuvants and vaccination routes usually induce dissimilar immune responses, and can even have completely opposite effects. The mechanism remains unclear. In this study, urease B subunit (UreB), an antigen of Helicobacter pylori (H. pylori) that can induce protective immune responses, was used as a model to vaccinate mice. We investigated the effects of different adjuvants and routes on consequent T cell epitope-specific targeting and protection against H...
July 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28747520/-68-ga-psma-pet-ct-impact-on-prostate-cancer-management
#17
Asim Afaq, Suliman Alahmed, Shih-Hsin Chen, Thabo Lengana, Athar Haroon, Heather Payne, Hashim Ahmed, Shonit Punwani, Mike Sathekge, Jamshed Bomanji
Aim: To assess the impact of (68)Ga-Prostate Specific Membrane Antigen (PSMA) Positron Emission Tomography/ Computed Tomography (PET/CT) on management of prostate cancer in patients with biochemical recurrence (BCR). Methods: Documented management plans were retrospectively reviewed before and after (68)Ga-PSMA PET/CT in 100 patients with BCR and change in plans recorded. Results: Management changed after (68)Ga-PSMA PET/CT in 39 patients (39%). These occurred in 23/68 (33.8%) of patients with radical prostatectomy (RP) and 16/32 (50%) of patients previously treated with radical radiotherapy...
July 26, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28747519/the-impact-of-adding-sentinel-node-biopsy-to-extended-pelvic-lymph-node-dissection-on-the-biochemical-recurrence-of-prostate-cancer-patients-treated-with-robot-assisted-radical-prostatectomy
#18
Nikolaos Grivas, Esther Wit, Teele Kuusk, Gijs KleinJan, Maarten Donswijk, Fijs van Leeuwen, Henk van der Poel
The benefit of adding sentinel node biopsy (SNB) to extended pelvic lymph node dissection (ePLND), remains controversial. Aim of our study was to evaluate biochemical recurrence (BCR) after robot-assisted radical prostatectomy (RARP) and ePLND in prostate cancer (PCa) patients, stratified by the application of SNB. The results were compared with the predictions of the updated Memorial Sloan Kettering Cancer Center (MSKCC) nomogram. Methods: Between January 2006 and November 2016, 920 patients underwent RARP with ePLND combined with or without SNB (184 and 736 patients, respectively)...
July 26, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28747501/mhv68-expressing-kshv-lana-reveals-both-functional-conservation-and-divergence-in-lana-homologs
#19
Arundhati Gupta, Darby G Oldenburg, Eduardo Salinas, Douglas W White, J Craig Forrest
Latency-associated nuclear antigen (LANA) is a multifunctional protein encoded by members of the Rhadinovirus genus of gammaherpesviruses. Studies using murine gammaherpesvirus 68 (MHV68) demonstrated that LANA is important for acute replication, latency establishment, and reactivation in vivo Despite structural similarities in their DNA-binding domains (DBDs), LANA homologs from Kaposi sarcoma-associated herpesvirus (KSHV) and MHV68 exhibit considerable sequence divergence. We sought to determine if KSHV and MHV68 LANA homologs are functionally interchangeable...
July 26, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28747172/characteristics-of-the-specific-humoral-response-in-patients-with-advanced-solid-tumors-after-active-immunotherapy-with-a-vegf-vaccine-at-different-antigen-doses-and-using-two-distinct-adjuvants
#20
Javier Sánchez Ramírez, Yanelys Morera Díaz, Mónica Bequet-Romero, Francisco Hernández-Bernal, Katty-Hind Selman-Housein Bernal, Ana de la Torre Santos, Eduardo Rafael Santiesteban Álvarez, Yenima Martín Bauta, Cimara H Bermúdez Badell, Josué de la Torre Pupo, Jorge V Gavilondo, Marta Ayala Avila
BACKGROUND: CIGB-247, a VSSP-adjuvanted VEGF-based vaccine, was evaluated in a phase I clinical trial in patients with advanced solid tumors (CENTAURO). Vaccination with the maximum dose of antigen showed an excellent safety profile, exhibited the highest immunogenicity and was the only one showing a reduction on platelet VEGF bioavailability. However, this antigen dose level did not achieve a complete seroconversion rate in vaccinated patients. These clinical results led us to the question whether a "reserve" of untapped immune response potential against VEGF could exist in cancer patients...
July 26, 2017: BMC Immunology
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