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Superoxide anion

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https://www.readbyqxmd.com/read/28806719/comparison-of-reactive-oxygen-species-metabolism-during-grape-berry-development-between-kyoho-and-its-early-ripening-bud-mutant-fengzao
#1
Fei-Fei Xi, Li-Li Guo, Yi-He Yu, Yan Wang, Qiong Li, Hui-Li Zhao, Guo-Hai Zhang, Da-Long Guo
Enzymes and non-enzyme elements related to the metabolism of reactive oxygen species (ROS), such as catalase (CAT), superoxide dismutase (SOD), ascorbic acid (AsA), glutathione (GSH), NADPH oxidase (NOX), hydrogen peroxide (H2O2), superoxide anion (O2(-)), lipoxygenase (LOX) and malondialdehyde (MDA), were measured in 'Kyoho' and its early ripening bud mutant 'Fengzao' to compare ROS level changes and investigate the potential roles of ROS in grape berry development and the ripening process. In addition, the anthocyanin and sugar contents as well as berry diameter were also investigated at different berry development stages...
August 9, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28804681/luminol-amplified-chemiluminescence-detects-mainly-superoxide-anion-produced-by-human-neutrophils
#2
Samia Bedouhène, Farida Moulti-Mati, Margarita Hurtado-Nedelec, Pham My-Chan Dang, Jamel El-Benna
Reactive oxygen species (ROS) are produced by numerous biological systems and by several phagocytes such as neutrophils and macrophages. ROS include mostly superoxide anion, hydrogen peroxide, singlet oxygen and hydroxyl radical, which are involved in a variety of biological processes such as immunity, inflammation, apoptosis and cell signaling. Thus, there is a need for a sensitive and reliable method to measure ROS. The luminol-amplified chemiluminescence technique is widely used to measure ROS production by neutrophils; however, it is unclear which ROS species are detected by this technique...
2017: American Journal of Blood Research
https://www.readbyqxmd.com/read/28797835/tumor-necrosis-factor-%C3%AE-receptor-1-contributes-to-ethanol-induced-vascular-reactive-oxygen-species-generation-and-hypertension
#3
Janaina A Simplicio, Natália A Gonzaga, Marcelo A Nakashima, Bruno S De Martinis, Thiago M Cunha, Luis F Tirapelli, Carlos R Tirapelli
We evaluated the contribution of tumor necrosis factor-α receptor 1 (TNFR1) to ethanol-induced hypertension and vascular oxidative stress and the possible role of perivascular adipose tissue (PVAT) in such responses. Male C57BL/6 wild-type (WT) or TNFR1-deficient mice (TNFR1(-/-)) were treated with ethanol (20% vol/vol) for 12 weeks. Ethanol induced an increase in blood pressure in WT mice and TNFR1(-/-) at 4 and 5 weeks of treatment, respectively. Treatment with ethanol increased tumor necrosis factor-α and interleukin-6 levels in aortas with or without PVAT (PVAT+ and PVAT-, respectively) from WT mice, but not TNFR1(-/-)...
July 22, 2017: Journal of the American Society of Hypertension: JASH
https://www.readbyqxmd.com/read/28795634/silencing-of-rbohf2-causes-leaf-age-dependent-accelerated-senescence-salicylic-acid-accumulation-and-powdery-mildew-resistance-in-barley
#4
Denise Pereira Torres, Reinhard Korbinian Proels, Harald Schempp, Ralph Huckelhoven
Plant RBOH (RESPIRATORY BURST OXIDASE HOMOLOGUES)-type NADPH oxidases produce superoxide radical anions and have a function in developmental processes and in response to environmental challenges. Barley RBOHF2 has different reported functions in interaction with the biotrophic powdery mildew fungus Blumeria graminis f.sp. hordei. Here we analysed in detail, plant leaf level- and leaf age-specific susceptibility of stably RBOHF2-silenced barley plants. This revealed enhanced susceptibility to fungal penetration of young RBOHF2-silenced leaf tissue but strongly reduced susceptibility of older leaves when compared to controls...
August 10, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28794327/a-reactive-oxygen-nitrogen-species-sensor-fabricated-from-an-electrode-modified-with-a-polymerized-iron-porphyrin-and-a-polymer-electrolyte-membrane
#5
Ryo Matsuoka, Chihiro Kobayashi, Atsushi Nakagawa, Shigeo Aoyagi, Tatsuo Aikawa, Takeshi Kondo, Shigenobu Kasai, Makoto Yuasa
We have developed an electrochemical reactive oxygen/nitrogen species sensor that can detect superoxide anion radicals (O2(-•)) and nitric oxide (NO). The reactive oxygen/nitrogen species sensor was fabricated by surface modification of an electrode with polymerized iron tetrakis(3-thienyl)porphyrin (FeT3ThP), and it can detect either O2(-•) or NO by switching the applied potential. Furthermore, we fabricated a sensor with improved selectivity by coating a Nafion(®) film onto the poly(FeT3ThP)-modified electrode...
2017: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/28793782/direct-activation-of-nadph-oxidase-2-by-2-deoxyribose-1-phosphate-triggers-nuclear-factor-kappa-b-dependent-angiogenesis
#6
Dina Vara, Joanna M Watt, Tiago M Fortunato, Harry Mellor, Matthew Burgess, Kate Wicks, Kimberly Mace, Shaun Reeksting, Anneke T Lubben, Caroline Pd Wheeler-Jones, Giordano Pula
AIMS: Deoxyribose-1-phosphate (dRP) is a pro-angiogenic paracrine stimulus released by cancer cells, platelets and macrophages and acting on endothelial cells. The objective of this study was to clarify how dRP stimulates angiogenic responses in human endothelial cells. RESULTS: Live cell imaging, electron paramagnetic resonance (EPR), pull-down of dRP-interacting proteins followed by immunoblotting, gene silencing of different NOXs and their regulatory co-subunits by siRNA transfection, and experiments with inhibitors of the sugar transporter GLUT1 were utilized to demonstrate that dRP acts intracellularly by directly activating the endothelial NADPH oxidase 2 (NOX2) complex, but not NOX4...
August 10, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28793197/glycosylation-with-o-linked-%C3%AE-n-acetylglucosamine-o-glcnac-induces-vascular-dysfunction-via-production-of-superoxide-anion-reactive-oxygen-species
#7
Leonardo Souza-Silva, Rhéure Alves-Lopes, Jéssica Silva Miguez, Vanessa Dela Justina, Karla Bianca Neves, Fabíola Leslie Mestriner, Rita C Tostes, Fernanda Regina Giachini, Victor V Lima
Overproduction of superoxide anion (•O2-) and O-linked β-N-acetylglucosamine (O-GlcNAc)-modification in the vascular system are contributors to endothelial dysfunction. This study tested the hypothesis that increased levels of O-GlcNAc-modified proteins contribute to •O2- production via activation of NADPH oxidase, resulting in impaired vasodilation. Rat aortic segments and vascular smooth muscle cell (VSMCs) were incubated with vehicle (methanol) or PUGNAc (100 µM). PUGNAc produced a time-dependent increase in O-GlcNAc levels in VSMC and decreased endothelium-dependent relaxation, which was prevented by apocynin and Tiron, suggesting that •O2- contributes to endothelial dysfunction under augmented O-GlcNAc levels...
August 9, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28781773/the-relationship-between-dietary-methionine-and-growth-digestion-absorption-and-antioxidant-status-in-intestinal-and-hepatopancreatic-tissues-of-sub-adult-grass-carp-ctenopharyngodon-idella
#8
Pei Wu, Ling Tang, Weidan Jiang, Kai Hu, Yang Liu, Jun Jiang, Shengyao Kuang, Ling Tang, Wuneng Tang, Yongan Zhang, Xiaoqiu Zhou, Lin Feng
BACKGROUND: Methionine is an essential amino acid for fish. The present study was conducted to investigate the effects of dietary methionine on growth performance, digestive and absorptive ability, as well as antioxidant capacity in the intestine and hepatopancreas of sub-adult grass carp (Ctenopharyngodon idella). RESULTS: Dietary methionine deficiency significantly decreased percentage weight gain (PWG), feed intake, feed efficiency and protein efficiency ratio, as well as activities of hepatopancreatic glutamate-oxaloacetate transaminase and muscle glutamate-pyruvate transaminase in sub-adult grass carp (P < 0...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28780636/photoconversion-of-2-chloronaphthalene-in-water
#9
Chunli Kang, Siqi Bao, Baiyan Chen, Yubo Zhong, Dongmei Huang, Yuhan Wang, Honghai Xue, Tao Tian
The photoconversion of 2-chloronaphthalene (CN-2) in water in a simulated sunlight system was investigated. The photoconversion efficiency, photoproducts and mechanisms were inspected, and the effects of inorganic ions (NO3(-), NO2(-)) and fulvic acid (FA) were discussed. The results showed that CN-2 could be transformed in water under the irradiation. NO3(-) and NO2(-) promoted the photoconversion of CN-2 owing to ·OH generated by the photolysis of NO3(-) and NO2(-); FA at a lower concentration promoted the photoconversion, but it had an inhibition effect at a higher concentration...
August 5, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28780446/biochemical-responses-to-cadmium-exposure-in-oncorhynchus-mykiss-erythrocytes
#10
Patrick Orlando, Sonia Silvestri, Enea Ferlizza, Giulia Andreani, Emilio Carpenè, Giancarlo Falcioni, Luca Tiano, Gloria Isani
Cd is known for its carcinogenic effects, however its mechanism of toxicity and in particular its ability to promote oxidative stress is debated. In fact, although it is considered a redox-inactive metal, at high concentration Cd was shown to promote indirectly oxidative stress. In this study we investigated metal accumulation in ex vivo exposed trout (Oncorhynchus mykiss) erythrocytes and Cd dose-dependent effect in terms of RBC viability, cytosolic and mitochondrial ROS levels as well as its effects on mitochondrial membrane depolarization, hemoglobin stability and precipitation...
August 3, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28780408/an-essential-role-of-n-terminal-domain-of-copper-chaperone-in-the-enzymatic-activation-of-cu-zn-superoxide-dismutase
#11
Mami Fukuoka, Eiichi Tokuda, Kenta Nakagome, Zhiliang Wu, Isao Nagano, Yoshiaki Furukawa
Cu/Zn-superoxide dismutase (SOD1) is an enzyme that disproportionates superoxide anion into hydrogen peroxide and molecular oxygen. The enzymatic activity of SOD1 requires the binding of copper and zinc ions and also the formation of a conserved intramolecular disulfide bond. In a eukaryotic cell, a copper chaperone for SOD1 (CCS) has been known to supply a copper ion and also introduce the disulfide bond into SOD1; however, a mechanism controlling the CCS-dependent activation of SOD1 remains obscure. Here, we characterized CCS isolated from a human liver fluke, Clonorchis sinensis, and found that an N-terminal domain of CCS was essential in supplying a copper ion in SOD1...
July 31, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28778044/role-of-phenolic-compounds-during-antioxidative-responses-of-winter-triticale-to-aphid-and-beetle-attack
#12
Paweł Czerniewicz, Hubert Sytykiewicz, Roma Durak, Beata Borowiak-Sobkowiak, Grzegorz Chrzanowski
One of the earliest responses of plants to insects' attack is generation of reactive oxygen species. However, the elevated level of ROS can elicit oxidative burst within plant tissues, and plants employ antioxidant systems against these radicals. Due to their chemical structures, polyphenols are able to diminish the level of ROS. Thus, we investigated the role of phenolic compounds in oxidative stress within winter triticale caused by Sitobion avenae and Oulema melanopus. It was found, that infestation by insects induced a high increase in the content of hydrogen peroxide and superoxide anion radical within resistant Lamberto cv...
July 29, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28777548/generation-and-detection-of-reactive-oxygen-species-in-photocatalysis
#13
Yoshio Nosaka, Atsuko Y Nosaka
The detection methods and generation mechanisms of the intrinsic reactive oxygen species (ROS), i.e., superoxide anion radical ((•)O2(-)), hydrogen peroxide (H2O2), singlet oxygen ((1)O2), and hydroxyl radical ((•)OH) in photocatalysis, were surveyed comprehensively. Consequently, the major photocatalyst used in heterogeneous photocatalytic systems was found to be TiO2. However, besides TiO2 some representative photocatalysts were also involved in the discussion. Among the various issues we focused on the detection methods and generation reactions of ROS in the aqueous suspensions of photocatalysts...
August 4, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28777255/endogenous-and-agonist-induced-opening-of-mitochondrial-big-vs-small-ca2-sensitive-k-channels-on-cardiac-cell-and-mitochondrial-protection
#14
David F Stowe, Meiying Yang, James S Heisner, Amadou K S Camara
Both big (BKCa) and small (SKCa) conductance Ca-sensitive K channels are present in mammalian cardiac cell mitochondria (m). We used pharmacological agonists and antagonists of BKCa and SKCa channels to examine the importance of endogenous opening of these channels and the relative contribution of either or both of these channels to protect against contractile dysfunction and reduce infarct size after ischemia reperfusion (IR) injury through a mitochondrial protective mechanism. Following global cardiac IR injury of ex vivo perfused guinea pig hearts we found the following: both agonists NS1619 (for BKCa) and DCEB (for SKCa) improved contractility; BKCa antagonist paxilline (PAX) alone or with SKCa antagonist NS8593 worsened contractility and enhanced infarct size; both antagonists PAX and NS8593 obliterated protection by their respective agonists; BKCa and SKCa antagonists did not block protection afforded by SKCa and BKCa agonists, respectively; and all protective effects by the agonists were blocked by scavenging superoxide anions (O2) with TBAP...
August 4, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28776941/ratiometric-fluorescence-detection-of-superoxide-anion-based-on-aunps-bsa-tb-gmp-nanoscale-coordination-polymers
#15
Nan Liu, Juan Hao, Keying Cai, Mulan Zeng, Zhenzhong Huang, Lili Chen, Bingxian Peng, Ping Li, Li Wang, Yonghai Song
A novel ratiometric fluorescence nanosensor for superoxide anion (O2(•-) ) detection was designed with gold nanoparticles-bovine serum albumin (AuNPs-BSA)@terbium/guanosine monophosphate disodium (Tb/GMP) nanoscale coordination polymers (NCPs) (AuNPs-BSA@Tb/GMP NCPs). The abundant hydroxyl and amino groups of AuNPs-BSA acted as binding points for the self-assembly of Tb(3+) and GMP to form core-shell AuNPs-BSA@Tb/GMP NCP nanosensors. The obtained probe exhibited the characteristic fluorescence emission of both AuNPs-BSA and Tb/GMP NCPs...
August 3, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28776450/pharmacological-modulation-of-reactive-oxygen-species-ros-improves-the-airway-hyperresponsiveness-by-shifting-the-th1-response-in-allergic-inflammation-induced-by-ovalbumin
#16
Renata Tiscoski Nesi, Mavina Valente Barroso, Valdirene de Souza Muniz, Ana Carolina de Arantes, Marco Aurélio Martins, Lycia de Brito Gitirana, Josiane Sabbadini Neves, Cláudia Farias Benjamim, Manuella Lanzetti, Samuel Santos Valença
Asthma is an allergic inflammation driven by the Th2 immune response with release of such cytokines as IL-4 and IL-13, which contribute to the airflow limitations and airway hyperresponsiveness (AHR). The involvement of oxidative stress in this process is well-established, but the specific role of the superoxide anion and nitric oxide in asthma are poorly understood. Thus, the aim of this study was to investigate the mechanisms underlying the superoxide anion/nitric oxide production and detoxification in a murine asthma model...
August 4, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28770648/effect-of-uric-acid-on-inflammatory-cox-2-and-ros-pathways-in-vascular-smooth-muscle-cells
#17
Nurgül Oğuz, Mustafa Kırça, Arzu Çetin, Akın Yeşilkaya
Hyperuricemia is thought to play a role in cardiovascular diseases (CVD), including hypertension, coronary artery disease and atherosclerosis. However, exactly how uric acid contributes to these pathologies is unknown. An underlying mechanism of inflammatory diseases, such as atherosclerosis, includes enhanced production of cyclooxygenase-2 (COX-2) and superoxide anion. Here, we aimed to examine the effect of uric acid on inflammatory COX-2 and superoxide anion production and to determine the role of losartan...
August 3, 2017: Journal of Receptor and Signal Transduction Research
https://www.readbyqxmd.com/read/28770609/a-dual-ratiometric-fluorescent-nanoprobe-for-visualizing-the-dynamic-process-of-ph-and-superoxide-anion-changes-in-autophagy-and-apoptosis
#18
Limin Yang, Yuanyuan Chen, Zhengze Yu, Wei Pan, Hongyu Wang, Na Li, Bo Tang
Autophagy and apoptosis are closely associated with various pathological and physiological processes in cell cycles. Investigating the dynamic changes of intracellular active molecules in autophagy and apoptosis is of great significance for clarifying their interrelationship and regulating mechanism in many diseases. In this study, we develop a dual-ratiometric fluorescent nanoprobe for quantitatively differentiating the dynamic process of superoxide anion (O2•-) and pH changes in autophagy and apoptosis in HeLa cells...
August 3, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28767746/exogenous-catalase-and-pyruvate-dehydrogenase-improve-survival-and-regeneration-and-affect-oxidative-stress-in-cryopreserved-dendrobium-nobile-protocorm-like-bodies
#19
W Di, M X Jia, J Xu, B L Li, Y Liu
BACKGROUND: Reactive oxygen species (ROS)-induced oxidative damage is responsible for viability loss in plant tissues following cryopreservation. Antioxidants may improve viability by preventing or repairing the injury. OBJECTIVE: This work aimed at studying the effect of catalase (CAT) and pyruvate dehydrogenase (PDH), which are involved in ROS metabolism and are differentially expressed during pollen cryopreservation, for cryopreservation of Dendrobium nobile Lindl...
May 2017: Cryo Letters
https://www.readbyqxmd.com/read/28764047/an-integrated-antioxidant-activity-fingerprint-for-commercial-teas-based-on-their-capacities-to-scavenge-reactive-oxygen-species
#20
Yu-Jie Guo, Li-Qiong Sun, Bo-Yang Yu, Jin Qi
An integrated antioxidant activity fingerprint, based on on-line screening methods for three reactive oxygen species (ROS: superoxide anion radical, hydrogen peroxide, and hydroxyl radical) was developed to comprehensively evaluate the quality of 12 batches of commercial tea. High-performance liquid chromatography (HPLC) coupled with a chemiluminescent detector was used to determine the antioxidant characteristics of a selection of teas as bioactivity fingerprints. An HPLC-electrospray ionization-mass spectrometry analysis was used to determine the chemical profiles of the teas in the chromatographic fingerprints...
December 15, 2017: Food Chemistry
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