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Superoxide anion

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https://www.readbyqxmd.com/read/28521553/glutaraldehyde-polymerized-hemoglobin-and-tempol-polyhb-tempol-has-superoxide-dismutase-activity-that-can-attenuate-oxidative-stress-on-endothelial-cells-induced-by-superoxide-anion
#1
Mengdi Wu, Kun Feng, Qiuhui Li, Huiya Ma, Hongli Zhu, Yudou Xie, Gaofei Yan, Chao Chen, Kunping Yan
A Tempol compound with an amine group (4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl, NH2-Tempol) was cross-linked to hemoglobin in a one-step polymerization reaction to produce a novel hemoglobin-based oxygen carrier (HBOC) designated PolyHb-Tempol. The reaction parameters, including the reaction time, pH, temperature, and ratio of reactants, were optimized, and the physiochemical properties of the resulting product were characterized. PolyHb-Tempol didn't show any toxicity towards endothelial cells. Furthermore, from observations of cell morphology and viability, PolyHb-Tempol showed a significant ability to inhibit or eliminate oxidative stress induced by superoxide free radicals...
May 18, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28516768/trimetallic-nitride-endohedral-fullerenes-carboxyl-gd3n-c80-a-new-theranostic-agent-for-combating-oxidative-stress-and-resolving-inflammation
#2
Tinghui Li, Li Xiao, Jiezuan Yang, Mengmeng Ding, Zhiguo Zhou, Leslie LaConte, Li Jin, Harry C Dorn, Xudong Li
Antioxidative and anti-inflammatory effects of trimetallic nitride endohedral fullerenes carboxyl-Gd3N@C80, a newly developed magnetic resonance imaging (MRI) contrast agent, were investigated. All hydrochalarone and carboxyl-functionalized fullerenes showed effective radical (hydroxyl and superoxide anion) scavenging, whereas the carboxyl-Gd3N@C80 more efficiently attenuated lipopolysaccharide (LPS) induced oxidative stress in macrophages. Carboxyl-Gd3N@C80 also suppressed LPS-elicited mRNA expression of pro-inflammatory inducible nitric oxide synthase and tumor necrosis factor-alpha, and upregulated antioxidative enzyme axis Nrf2 and heme oxygenase-1, possibly via ERK but not AKT signaling pathways...
May 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28508104/quercetin-inhibits-gout-arthritis-in-mice-induction-of-an-opioid-dependent-regulation-of-inflammasome
#3
Kenji W Ruiz-Miyazawa, Larissa Staurengo-Ferrari, Sandra S Mizokami, Talita P Domiciano, Fabiana T M C Vicentini, Doumit Camilios-Neto, Wander R Pavanelli, Phileno Pinge-Filho, Flávio A Amaral, Mauro M Teixeira, Rubia Casagrande, Waldiceu A Verri
We investigated the anti-inflammatory and analgesic effects of quercetin in monosodium urate crystals (MSU)-induced gout arthritis, and the sensitivity of quercetin effects to naloxone, an opioid receptor antagonist. Mice were treated with quercetin, and mechanical hyperalgesia was assessed at 1-24 h after MSU injection. In vivo, leukocyte recruitment, cytokine levels, oxidative stress, NFκB activation, and gp91(phox) and inflammasome components (NLRP3, ASC, Pro-caspase-1, and Pro-IL-1β) mRNA expression by qPCR were determined in the knee joints at 24 h after MSU injection...
May 15, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/28504877/illuminating-superoxide-anion-and-ph-enhancements-in-apoptosis-of-breast-cancer-cells-induced-by-mitochondrial-hyperfusion-using-a-new-two-photon-fluorescence-probe
#4
Wen Zhang, Xin Wang, Ping Li, Haibin Xiao, Wei Zhang, Hui Wang, Bo Tang
Mitochondrial morphology regulated by fusion and fission processes determines mitochondrial function and cell fate. Some studies showed hyperfused mitochondria could induce apoptosis in cancer cells, but the relevant molecular mechanisms remain elusive. Superoxide (O2•-) and pH play vital roles in mitochondrial dysfunction and apoptosis. Therefore, it is worthwhile to explore if there is an intimate relationship between mitochondrial hyperfusion and simultaneous changes in O2•- and pH levels, which will be helpful to uncover relevant detailed mechanism...
May 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28503850/roles-of-spermine-in-modulating-the-antioxidant-status-and-nrf2-signalling-molecules-expression-in-the-thymus-and-spleen-of-suckling-piglets-new-insight
#5
W Cao, X Xu, G Jia, H Zhao, X Chen, C Wu, J Tang, J Wang, J Cai, G Liu
This study aimed to investigate the effects of spermine and extended spermine administration on the antioxidant status and expression of NF-E2-related factor 2 (Nrf2) signalling molecules in the thymus and spleen in suckling piglets. One half of eighty 12-day-old suckling piglets obtained sufficient nutrient intake supplemented with spermine (0.4 mmol/kg body weight), and another half received restricted nutrient intake supplemented with physiological saline in equal doses once a day for 7 hr or 3, 6 or 9 days in pairs...
May 15, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28501692/trans-chalcone-added-in-topical-formulation-inhibits-skin-inflammation-and-oxidative-stress-in-a-model-of-ultraviolet-b-radiation-skin-damage-in-hairless-mice
#6
Renata M Martinez, Felipe A Pinho-Ribeiro, David L Vale, Vinicius S Steffen, Fabiana T M C Vicentini, Josiane A Vignoli, Marcela M Baracat, Sandra R Georgetti, Waldiceu A Verri, Rubia Casagrande
Trans-chalcone (TC) is a common precursor of flavonoids. However, the pharmacological properties of TC remain to be fully understood. The present study investigated whether topical formulation containing TC (TFcTC) presents therapeutic effect in UVB radiation-induced skin damage using disease, enzyme activity, antioxidant activity, protein and mRNA parameters. Control topical formulation (CTF) and TFcTC were applied in hairless mice before and after exposure to UVB radiation. Dorsal skin samples were collected after UVB exposure to evaluate: i) skin edema (weight) was measured by punch biopsy; ii) spectrophotometric assays were used to measure myeloperoxidase (MPO) and catalase activities, ferric (FRAP) and ABTS cation reducing antioxidant power, superoxide anion production and levels of reduced glutathione (GSH); iii) enzymography was used to measure matrix metalloproteinase-9 (MMP-9) activity; iv) chemiluminescence was used to measure the lipid peroxidation (LPO); v) enzyme-linked immunosorbent assay (ELISA) was used to measure tumor necrosis factor alpha (TNF-α) levels; vi) reverse transcription quantitative PCR (RT-qPCR) was used to measure cyclooxygenase-2 (COX-2), gp91(phox) (NADPH oxidase sub-unity), glutathione peroxidase-1 (Gpx1), glutathione reductase (Gr), nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) mRNA expression...
May 2, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28499147/fructose-increases-corticosterone-production-in-association-with-nadph-metabolism-alterations-in-rat-epididymal-white-adipose-tissue
#7
Paula D Prince, Yanina A Santander, Estefania M Gerez, Christian Höcht, Ariel H Polizio, Marcos A Mayer, Carlos A Taira, Cesar G Fraga, Monica Galleano, Andrea Carranza
Metabolic syndrome is an array of closely metabolic disorders that includes glucose intolerance/insulin resistance, central obesity, dyslipidemia, and hypertension. Fructose, a highly lipogenic sugar, has profound metabolic effects in adipose tissue, and has been associated with the etiopathology of many components of the metabolic syndrome. In adipocytes, the enzyme 11 β-HSD1 amplifies local glucocorticoid production, being a key player in the pathogenesis of central obesity and metabolic syndrome. 11 β-HSD1 reductase activity is dependent on NADPH, a cofactor generated by H6PD inside the endoplasmic reticulum...
April 6, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28493961/aldosterone-signaling-regulates-the-over-expression-of-claudin-4-and-8-at-the-distal-nephron-from-type-1-diabetic-rats
#8
Eduardo Molina-Jijón, Rafael Rodríguez-Muñoz, Ricardo González-Ramírez, Carmen Namorado-Tónix, José Pedraza-Chaverri, Jose L Reyes
Hyperglycemia in diabetes alters tight junction (TJ) proteins in the kidney. We evaluated the participation of aldosterone (ALD), and the effect of spironolactone (SPL), a mineralocorticoid receptor antagonist, on the expressions of claudin-2, -4, -5 and -8, and occludin in glomeruli, proximal and distal tubules isolated from diabetic rats. Type 1 diabetes was induced in female Wistar rats by a single tail vein injection of streptozotocin (STZ), and SPL was administrated daily by gavage, from days 3-21. Twenty-one days after STZ injection the rats were sacrificed...
2017: PloS One
https://www.readbyqxmd.com/read/28493845/histopathological-findings-of-renal-tissue-induced-by-oxidative-stress-due-to-different-concentrations-of-fluoride
#9
Qin Luo, Hengmin Cui, Huidan Deng, Ping Kuang, Huan Liu, Yujiao Lu, Jing Fang, Zhicai Zuo, Junliang Deng, Yinglun Li, Xun Wang, Ling Zhao
It has been reported that excessive intake of fluoride can induce renal lesions. However, its pathogenesis is still less understood. Therefore, this study was conducted to investigate oxidative damage and the relationships between the oxidative damage and renal lesions in fluoride-treated mice by using the methods of histopathology, biochemistry, flow cytometry and quantitative real-time polymerase chain reaction (qRT-PCR). A total of 240 ICR mice were randomly divided into four equal groups (sodium fluoride was given orally at the dose of 0, 12, 24 and 48 mg/kg body weight for 42 days, respectively)...
April 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28491113/antioxidant-anti-inflammatory-and-analgesic-activities-of-agrimonia-eupatoria-l-infusion
#10
Telmo N Santos, Gustavo Costa, J Pinto Ferreira, Joana Liberal, Vera Francisco, António Paranhos, Maria T Cruz, M Castelo-Branco, I Vitória Figueiredo, M Teresa Batista
Agrimony (Agrimonia eupatoria L.) (Ae) is used in traditional medicine to treat inflammatory and oxidative related diseases. Therefore, this study focuses on the anti-inflammatory and analgesic potential of Ae infusion (AeI). Phenolic compounds characterization was achieved by HPLC-PDA-ESI/MS (n) . To evaluate antioxidant potential, 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radical, and SNAP assays were used. In vitro anti-inflammatory activity of AeI was investigated in LPS-stimulated macrophages by measuring the NO production...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28490136/antioxidative-capacity-and-binding-affinity-of-the-complex-of-green-tea-catechin-and-beta-lactoglobulin-glycated-by-the-maillard-reaction
#11
Marija Perusko, Ayah Al-Hanish, Jelena Mihailovic, Simeon Minic, Sara Trifunovic, Ivana Prodic, Tanja Cirkovic Velickovic
Major green tea catechin, epigallocatechin-3-gallate (EGCG), binds non-covalently to numerous dietary proteins, including beta-lactoglobulin of cow's milk. The effects of glycation of proteins via Maillard reaction on the binding capacity for polyphenols and the antiradical properties of the formed complexes have not been studied previously. Binding constant of BLG glycated by milk sugar lactose to EGCG was measured by the method of fluorophore quenching. Binding of EGCG was confirmed by CD and FTIR. The antioxidative properties of the complexes were examined by measuring ABTS radical scavenging capacity, superoxide anion scavenging capacity and total reducing power assay...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28474554/flavonoid-enriched-fraction-of-campylandra-aurantiaca-attenuates-carbon-tetrachloride-induced-oxidative-dna-damage-in-mouse-peritoneal-macrophages-in-animal-model
#12
Mainak Chakraborty, Asis Bala, Pallab K Haldar
The aim of the present study was to evaluate the effect of flavonoid enriched fraction of Campylandra aurantiaca (FEFCA) on in vitro and in vivo antioxidant and DNA protective effect in mouse peritoneal macrophages cells. FEFCA was characterized by HPLC analysis. The in vitro antioxidant activities of FEFCA was measured by different in vitro assays like 1, 1-diphenyl-2-picrylhydrazil radical (DPPH), superoxide anions, nitric oxide and hydroxyl radicals scavenging methods. Isolated mouse peritoneal macrophages were oxidized by carbon tetra chloride (CCl4) in animal model; subsequently the protective effect of FEFCA was determined in terms of estimation of antioxidant enzyme and the damage to DNA of the cells...
May 3, 2017: Current Drug Discovery Technologies
https://www.readbyqxmd.com/read/28463730/antioxidant-responses-of-peanut-roots-exposed-to-realistic-groundwater-doses-of-arsenate-identification-of-glutathione-s-transferase-as-a-suitable-biomarker-for-metalloid-toxicity
#13
Eliana Bianucci, Ana Furlan, María Del Carmen Tordable, Luis E Hernández, Ramón O Carpena-Ruiz, Stella Castro
Arsenic (As)-polluted groundwater constitutes a serious problem for peanut plants, as roots can accumulate the metalloid in their edible parts. Characterization of stress responses to As may help to detect potential risks and identify mechanisms of tolerance, being the induction of oxidative stress a key feature. Fifteen-day old peanut plants were treated with arsenate in order to characterize the oxidative stress indexes and antioxidant response of the legume under realistic groundwater doses of the metalloid...
April 24, 2017: Chemosphere
https://www.readbyqxmd.com/read/28461152/substrate-analog-binding-and-electrostatic-surfaces-of-human-manganese-superoxide-dismutase
#14
Jahaun Azadmanesh, Scott R Trickel, Gloria E O Borgstahl
Superoxide dismutases (SODs) are enzymes that play a key role in protecting cells from toxic oxygen metabolites by disproportionation of two molecules of superoxide into molecular oxygen and hydrogen peroxide via cyclic reduction and oxidation at the active site metal. The azide anion is a potent competitive inhibitor that binds directly to the metal and is used as a substrate analog to superoxide in studies of SOD. The crystal structure of human MnSOD-azide complex was solved and shows the putative binding position of superoxide, providing a model for binding to the active site...
April 29, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28459518/antioxidant-and-hepatoprotective-activities-of-dicoma-anomala-sond-aqueous-root-extract-against-carbon-tetrachloride-induced-liver-damage-in-wistar-rats
#15
F O Balogun, A O T Ashafa
OBJECTIVE: To evaluates the antioxidant and hepatoprotective potentials of Dicoma anomala Sond. (Asteraceae) on body weight, feed and water intake, biochemical parameters and organ histology. METHODS: Various concentrations (1.56-25 μg/mL) were used in the in vitro assays 1,1-diphenyl-2-picryl hydrazyl (DPPH, superoxide anion, hydroxyl radicals, etc.). The effects of treatment with 125, 250 and 250 mg/mL Dicoma anomala aqueous roots extract (DARE) was investigated in vivo in the CCl4-induced hepatotoxic rats during the 15 days study...
August 2016: Journal of Traditional Chinese Medicine, Chung i Tsa Chih Ying Wen Pan
https://www.readbyqxmd.com/read/28456571/erythropoietin-activates-sirt1-to-protect-human-cardiomyocytes-against-doxorubicin-induced-mitochondrial-dysfunction-and-toxicity
#16
Lan Cui, Jiabin Guo, Qiang Zhang, Jian Yin, Jin Li, Wei Zhou, Tingfen Zhang, Haitao Yuan, Jun Zhao, Li Zhang, Paul L Carmichael, Shuangqing Peng
The hormone erythropoietin (EPO) has been demonstrated to protect against chemotherapy drug doxorubicin (DOX)-induced cardiotoxicity, but the underlying mechanism remains obscure. We hypothesized that silent mating type information regulation 2 homolog 1 (SIRT1), an NAD(+)-dependent protein deacetylase that activates peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), plays a crucial role in regulating mitochondrial function and mediating the beneficial effect of EPO. Our study in human cardiomyocyte AC16 cells showed that DOX-induced cytotoxicity and mitochondrial dysfunction, as manifested by decreased mitochondrial DNA (mtDNA) copy number, mitochondrial membrane potential, and increased mitochondrial superoxide accumulation, can be mitigated by EPO pretreatment...
April 27, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28455949/detection-of-intracellular-reactive-oxygen-species-superoxide-anion-and-hydrogen-peroxide-and-lipid-peroxidation-during-cryopreservation-of-alpaca-spermatozoa
#17
S Evangelista-Vargas, A Santiani
The objective of this study was to detect changes in intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) production and lipid peroxidation during cryopreservation of alpaca spermatozoa. Twelve alpaca semen samples were conventionally cryopreserved. Intracellular superoxide anion and hydrogen peroxide were evaluated by fluorescence microscopy using dihydroethidium (DHE)/YO-PRO-1 and dichlorofluorescein diacetate (H2DCFDA)/propidium iodide (PI), respectively. Evaluations were performed during cooling curve at (1) 25°C, (2) 15°C, (3) 5°C/0 min, (4) 5°C/15 min, (5) 5°C/30 min and (6) after freezing/thawing...
April 29, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28453981/facile-synthesis-of-flower-like-ag3vo4-bi2wo6-heterojunction-with-enhanced-visible-light-photocatalytic-activity
#18
Shijie Li, Shiwei Hu, Wei Jiang, Yu Liu, Jianshe Liu, Zhaohui Wang
Constructing novel semiconductor heterojunctions is one of the most significant approaches to improving the photocatalytic performance of a photocatalyst. Herein, the Ag3VO4/Bi2WO6 heterojunction was prepared through in-situ anchoring Ag3VO4 nanoparticles (size: ∼21nm) on the surface of Bi2WO6 microflowers (diameter: 2.5-4.5μm) by a facile deposition route. The photocatalytic activity of these heterojunctions were studied by decomposing cationic dye rhodamine B (RhB), anionic dye methyl orange (MO) and neutral para-chlorophenol (4-CP) under visible light irradiation (λ>400nm)...
April 20, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28452248/response-of-aquatic-protists-to-electric-field-exposure
#19
Marko Miliša, Domagoj Đikić, Tvrtko Mandić, Dino Grozić, Ivan Čolić, Ana Ostojić
PURPOSE: To test the effects of short term exposure of aquatic organisms to electric field (EF) with negligible magnetic component. MATERIALS AND METHODS: We built a plate capacitor that served as a source of EF of strengths that can be found in nature near transmission lines. We exposed two cultured protist species Euglena viridis and Paramecium caudatum to EFs for 24 hours and monitored their abundance, morphology, intracellular superoxide anion (by DHE), hydrogen peroxide by (H2DCF) and MDA contents, catalase (CAT) and superoxide dismutase (SOD) activity...
April 28, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28447385/ultrasound-enhances-calcium-absorption-of-jujube-fruit-by-regulating-the-cellular-calcium-distribution-and-metabolism-of-cell-wall-polysaccharides
#20
Huanhuan Zhi, Qiqi Liu, Juan Xu, Yu Dong, Mengpei Liu, Wei Zong
BACKGROUND: Ultrasound has been applied in fruit pre-washing processes. However, it is not sufficient to protect fruit from pathogenic infection throughout the entire storage period, and sometimes ultrasound causes tissue damage. The goal of this study was to investigate the effects of calcium chloride (10 g L(-1) ) and ultrasound (350 W at 40 kHz), separately and in combination, on fruit quality, antioxidant status, tissue Ca(2+) content and distribution along with cell wall metabolism at 20 °C for six days...
April 26, 2017: Journal of the Science of Food and Agriculture
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