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https://www.readbyqxmd.com/read/29684935/facial-asymmetry-based-age-group-estimation-role-in-recognizing-age-separated-face-images
#1
Muhammad Sajid, Imtiaz Ahmad Taj, Usama Ijaz Bajwa, Naeem Iqbal Ratyal
Face recognition aims to establish the identity of a person based on facial characteristics. On the other hand, age group estimation is the automatic calculation of an individual's age range based on facial features. Recognizing age-separated face images is still a challenging research problem due to complex aging processes involving different types of facial tissues, skin, fat, muscles, and bones. Certain holistic and local facial features are used to recognize age-separated face images. However, most of the existing methods recognize face images without incorporating the knowledge learned from age group estimation...
April 23, 2018: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/29684677/human-stem-cell-based-corneal-tissue-mimicking-structures-using-laser-assisted-3d-bioprinting-and-functional-bioinks
#2
Anni Sorkio, Lothar Koch, Laura Koivusalo, Andrea Deiwick, Susanna Miettinen, Boris Chichkov, Heli Skottman
There is a high demand for developing methods to produce more native-like 3D corneal structures. In the present study, we produced 3D cornea-mimicking tissues using human stem cells and laser-assisted bioprinting (LaBP). Human embryonic stem cell derived limbal epithelial stem cells (hESC-LESC) were used as a cell source for printing epithelium-mimicking structures, whereas human adipose tissue derived stem cells (hASCs) were used for constructing layered stroma-mimicking structures. The development and optimization of functional bioinks was a crucial step towards successful bioprinting of 3D corneal structures...
April 16, 2018: Biomaterials
https://www.readbyqxmd.com/read/29684322/modified-beacon-probe-assisted-dual-signal-amplification-for-visual-detection-of-microrna
#3
Xiuwei Sun, Na Ying, Chuanjing Ju, Zhongyi Li, Na Xu, Guijuan Qu, Wensen Liu, Jiayu Wan
In a recent study, we reported a novel assay for the detection of microRNA-21 based on duplex-specific nuclease (DSN)-assisted isothermal cleavage and hybridization chain reaction (HCR) dual signal amplification. The Fam modified double-stranded DNA products were generated after the HCR, another biotin modified probe was digested by DSN and released from the magnetic beads after the addition of the target miRNA. The released sequence was then combined with HCR products to form a double-tagging dsDNA, which can be recognized by the lateral flow strips...
April 20, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29684038/murine-embryos-exposed-to-human-endometrial-mscs-derived-extracellular-vesicles-exhibit-higher-vegf-pdgf-aa-release-increased-blastomere-count-and-hatching-rates
#4
Rebeca Blázquez, Francisco Miguel Sánchez-Margallo, Verónica Álvarez, Elvira Matilla, Nuria Hernández, Federica Marinaro, María Gómez-Serrano, Inmaculada Jorge, Javier G Casado, Beatriz Macías-García
Endometrial Mesenchymal Stromal Cells (endMSCs) are multipotent cells with immunomodulatory and pro-regenerative activity which is mainly mediated by a paracrine effect. The exosomes released by MSCs have become a promising therapeutic tool for the treatment of immune-mediated diseases. More specifically, extracellular vesicles derived from endMSCs (EV-endMSCs) have demonstrated a cardioprotective effect through the release of anti-apoptotic and pro-angiogenic factors. Here we hypothesize that EV-endMSCs may be used as a co-adjuvant to improve in vitro fertilization outcomes and embryo quality...
2018: PloS One
https://www.readbyqxmd.com/read/29683655/alkaline-phosphatase-assay-based-on-the-chromogenic-interaction-of-diethanolamine-with-4-aminophenol
#5
Jian Sun, Jiahui Zhao, Xingfu Bao, Qifeng Wang, Xiurong Yang
Diethanolamine (DEA) has been extensively utilized as an alkaline buffer in current assays of alkaline phosphatase (ALP) activity in the past decades. While playing the role of a buffer, DEA has been widely ignored about its chemical reactivity in such assays. Herein, we report an interesting chromogenic interaction between DEA and 4-aminophenol (AP) in the presence of H2 O for the first time, which inspires us to develop a novel DEA-participated ALP activity assay by using 4-aminophenyl phos-phate (APP) as a substrate...
April 23, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29683233/living-apart-or-together-neighbours-views-and-experiences-on-their-relationships-with-neighbours-with-and-without-intellectual-disabilities
#6
Tessa Overmars-Marx, Barbara Pepping, Fleur Thomése
BACKGROUND: Neighbours play an important role in the social inclusion of people with intellectual disabilities. Neighbouring in general might help understand the social interactions between neighbours with and without intellectual disabilities. Our study focuses on gaining insight into neighbouring patterns and how people with intellectual disabilities fit in them. METHOD: 26 interviews were conducted with 29 neighbours of people with intellectual disabilities on their norms and behaviours towards neighbours with and without disabilities...
April 23, 2018: Journal of Applied Research in Intellectual Disabilities: JARID
https://www.readbyqxmd.com/read/29682970/intermolecular-oxidative-radical-addition-to-aromatic-aldehydes-direct-access-to-1-4-and-1-5-diketones-via-silver-catalyzed-ring-opening-acylation-of-cyclopropanols-and-cyclobutanols
#7
Chao Che, Zhaosheng Qian, Mingchang Wu, Ying Zhao, Gangguo Zhu
A novel silver-catalyzed ring-opening acylation of cyclopropanols and cyclobutanols is described. The reaction proceeds under mild and neutral conditions and provides a facile access to nonsymmetric 1,4- and 1,5-diketones in promising yields with broad substrate scope. Mechanistic studies including DFT calculations suggest the involvement of an uncommon water-assisted 1,2-HAT process, which is strongly exothermic and thus promotes addition of carbon radicals to aldehydes. In contrast to traditional reductive radical addition protocols, this work represents the first example on the intermolecular oxidative radical addition to aldehydes, thus offering a novel strategy for the direct synthesis of acyclic ketones from readily accessible aldehydes...
April 23, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29682612/salvage-pelvic-lymph-node-dissection-after-fluciclovine-positron-emission-tomography-computed-tomography-detected-prostate-cancer-recurrence
#8
Madeline Cancian, Jorge Pereira, Joseph F Renzulli
Background: Multiple new systemic agents have been targeted to metastatic prostate cancer, with decreased progression of disease but no cure. Surgical management of metastatic disease has been gaining interest, primarily in the setting of high-risk prostatectomies. However, metastasis-directed surgical intervention has been employed in rare scenarios, especially in oligometastatic disease. We report here on a salvage robot-assisted pelvic lymph node dissection for a solitary metastatic site. Case Presentation: A 63-year-old Hispanic man who was initially treated with prostatectomy for intermediate risk cancer developed rapid biochemical recurrence...
2018: Journal of Endourology Case Reports
https://www.readbyqxmd.com/read/29682473/uniportal-thoracoscopic-resection-of-intralobar-and-extralobar-pulmonary-sequestration
#9
Andrea Dell'Amore, Domenica Giunta, Alessio Campisi, Stefano Congiu, Giampiero Dolci, Niccolò Antonino Barbera, Roberto Agosti, Francesco Buia
Pulmonary sequestration (PS) is a rare congenital malformation of the respiratory tract. Two main variants are described, the intralobar and the extralobar PS. Clinical manifestations vary from accidental findings to life threatening complications. Surgical resection is the definitive and indicated treatment of PS. The operation could be performed through an open thoracotomy or video-assisted thoracic surgery approach. We report the management of two patients with diagnosis of extralobar PS in the first case and intralobar PS in the second case...
2018: Journal of Visualized Surgery
https://www.readbyqxmd.com/read/29682467/extended-uniportal-video-assisted-thoracic-surgery-for-lung-cancer-is-it-feasible
#10
REVIEW
Iñigo Royo-Crespo, Arthur Vieira, Paula A Ugalde
Since the first description of uniportal video-assisted thoracic surgery (U-VATS) (or single-port) lobectomy, several centers in Asia and Europe rapidly adopted this technique as a standard approach for treatment of early stage non-small cell lung cancer (NSCLC). Despite the controversies regarding feasibility and completeness of resection, thoracic surgeons in high volume centers keep pushing the limits to perform very complex procedures also known as "extended resections" through minimally invasive surgery...
2018: Journal of Visualized Surgery
https://www.readbyqxmd.com/read/29682458/robot-assisted-thoracoscopic-right-upper-bi-lobectomy-for-pulmonary-tuberculosis
#11
Piotr Yablonskii, Grigorii Kudriashov, Larisa Kiryukhina, Armen Avetisyan
Surgery is one of the current options in cases of pulmonary tuberculosis with failed medication treatment. Lobectomy and bi-lobectomy constitute up to one third of all surgical procedures in such cases. Nevertheless, fibrotic changes near the hilar structures and lymph nodes are limitation factors for spread of video-assisted thoracoscopic surgery (VATS) anatomical pulmonary resection. Robotic surgery can reduce some disadvantages of VATS. This is well illustrated by the large pulmonary resections. Current case report showed first robot-assisted thoracoscopic (RATS) bi-lobectomy for pulmonary tuberculosis with good results 1-year follow-up...
2018: Journal of Visualized Surgery
https://www.readbyqxmd.com/read/29681570/single-femoral-venous-drainage-versus-both-vena-cava-drainage-in-isolated-repeat-tricuspid-valve-surgery
#12
Runsheng Peng, Hui Shi, Jun Ba, Chunsheng Wang
To assess the potential advantages of minimally invasive surgery using a single femoral venous drainage method versus femoral venous and superior vena cava or jugular venous drainage method during repeat tricuspid valve surgery.From January 2010 to December 2016, 50 repeat tricuspid valve procedures were performed using a minimally invasive approach without aortic cross-clamping at our institution. The arterial cannula was inserted into the femoral artery, and at the same time, the venous cannula was placed in the femoral vein in 28 patients (FV group) during cardiopulmonary bypass (CPB)...
April 20, 2018: International Heart Journal
https://www.readbyqxmd.com/read/29681493/are-radiographic-and-direct-measures-of-acetabular-polyethylene-wear-comparable
#13
Krista K Parran, Christopher P Bechtel, Rebecca D Moore, Jeremy J Gebhart, Michael S Reich, Matthew J Kraay, Clare M Rimnac
BACKGROUND: All polyethylene acetabular liners wear over time, and numerous methods for calculating linear wear rates exist. The objective of this study was to compare 2-dimensional wear rates between direct, micrometer measurements and the computerized, edge-detection method using Hip Analysis Suite (HAS) 8.0.4.3. METHODS: Two groups of retrieved acetabular liners from Harris-Galante Prosthesis I and Harris-Galante Prosthesis II implants in situ for more than 10 years were evaluated...
March 27, 2018: Journal of Arthroplasty
https://www.readbyqxmd.com/read/29681278/electrochemical-detection-of-sequence-specific-dna-based-on-formation-of-g-quadruplex-hemin-through-continuous-hybridization-chain-reaction
#14
Xiaofan Sun, Haohan Chen, Shuling Wang, Yiping Zhang, Yaping Tian, Nandi Zhou
A high-sensitive detection of sequence-specific DNA was established based on the formation of G-quadruplex-hemin complex through continuous hybridization chain reaction (HCR). Taking HIV DNA sequence as an example, a capture probe complementary to part of HIV DNA was firstly self-assembled onto the surface of Au electrode. Then a specially designed assistant probe with both terminals complementary to the target DNA and a G-quadruplex-forming sequence in the center was introduced into the detection solution...
August 27, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29681179/-femtosecond-laser-assisted-keratoplasty
#15
László Módis, Eszter Szalai, Zsuzsanna Flaskó, Bence Kolozsvári, András Berta
INTRODUCTION AND AIM: To present our results on femtosecond laser-assisted penetrating keratoplasty. PATIENTS AND METHOD: Twenty-five eyes of 25 patients underwent surgery with the following indications: pseudophakic bullous keratopathy (n = 10), keratoconus (n = 4), corneal dystrophy (n = 5), corneal scar (n = 4), band keratopathy (n = 2). Trephination of both the donor and recipient corneas were performed with VisuMax femtosecond laser device (Carl Zeiss Meditec AG, Jena, Germany)...
April 2018: Orvosi Hetilap
https://www.readbyqxmd.com/read/29680611/production-of-polyimide-ceria-nanocomposites-by-development-of-molecular-hook-technology-in-nano-sonochemistry
#16
Mehdi Hatami
Poly(amic acid), the precursor of polyimide (PI), was used for the preparation of PI/CeO2 nanocomposites (NC)s by ultrasonic assisted technique via insertion of the surface modified CeO2 nanoparticles (NP)s into PI matrix. In the preparation stages, in the first, the modifications of CeO2 NPs by using hexadecyltrimethoxysilane (HDTMS) as a binder were targeted using ultrasonic waves. In the second step, newly designed PI structure was formed from the sonochemical imidization process as a molecular hook. In this step two different reactions were occurred...
June 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29680601/impact-of-ultrasound-on-galactooligosaccharides-and-gluconic-acid-production-throughout-a-multienzymatic-system
#17
Fabián Rico-Rodríguez, Juan Carlos Serrato, Antonia Montilla, Mar Villamiel
Galactooligosaccharides (GOS), recognised prebiotic, can be industrially produced from lactose and commercial β-galactosidase (β-gal) from Kluyveromyces lactis. Residual lactose and glucose limit GOS applications. To handle this problem, a multienzymatic system, with β-gal and glucose oxidase (Gox), was proposed to reduce glucose content in reaction media through its oxidation to gluconic acid (GA). Besides, ultrasound (US) probe effect over the multienzymatic system to produce GOS and GA has been evaluated...
June 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29680598/ultrasound-electrospinning-assisted-fabrication-and-sensing-evaluation-of-a-novel-membrane-as-ultrasensitive-sensor-for-copper-ii-ions-detection-in-aqueous-environment
#18
Wei Gao, Pouya Haratipour, Mohammad Reza Rezaei Kahkha, Arash Tahvili
The present study has reported an optimized fabrication and application of a novel PVA/TEOS/Schiff base nanofibers membrane as a highly sensitive copper (II) ions in aqueous environment. Here in, for first time, an ultrasound-assisted synthesized symmetric Schiff base has been immobilized on a hybrid polyvinyl alcohol (PVA) and TEOS using electrospinning technique for detection and filtration of copper ions. For this purpose, various working parameters were evaluated and finally the optimized nano fibers membrane was synthesized with 72 nm thickness and PVA/TEOS/Schiff base ratio of (wt%) 8:6:1...
June 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29680241/the-structure-function-relationship-in-antimicrobial-peptides-what-can-we-obtain-from-structural-data
#19
Marlon H Cardoso, Karen G N Oshiro, Samilla B Rezende, Elizabete S Cândido, Octávio L Franco
Antimicrobial peptides (AMPs) have been widely isolated from most organisms in nature. This class of antimicrobials may undergo changes in their sequence for improved physicochemical properties, including charge, hydrophobicity, and hydrophobic moment. It is known that such properties may be directly associated with AMPs' structural arrangements and, consequently, could interfere in their modes of action against microorganisms. In this scenario, biophysical methodologies, such as nuclear magnetic resonance spectroscopy, X-ray crystallography, and cryo-electron microscopy, allied to in silico approaches, including molecular modeling, docking, and dynamics nowadays represent an enormous first step for the structural elucidation of AMPs, leading to further structure-function annotation...
2018: Advances in Protein Chemistry and Structural Biology
https://www.readbyqxmd.com/read/29680038/impact-of-electrical-conductivity-on-acid-hydrolysis-of-guar-gum-under-induced-electric-field
#20
Dandan Li, Yao Zhang, Na Yang, Zhengyu Jin, Xueming Xu
This study aimed to improve induced electric field (IEF)-assisted hydrolysis of polysaccharide by controlling electrical conductivity. As the conductivity of reaction medium was increased, the energy efficiency of IEF was increased because of deceased impedance, as well as enhanced output voltage and temperature, thus the hydrolysis of guar gum (GG) was accelerated under IEF. Changes in weight-average molecular weight (Mw) suggested that IEF-assisted hydrolysis of GG could be described by the first-order kinetics 1/Mw ∝ kt, with the rate constant (k), varying directly with the medium conductivity...
September 1, 2018: Food Chemistry
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