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lyophilized plasma

Zhanglong Peng, Shibani Pati, Magali J Fontaine, Kelly Hall, Anthony V Herrera, Rosemary A Kozar
BACKGROUND: Clinical studies have demonstrated that the early and empiric use of plasma improves survival after hemorrhagic shock. We have demonstrated in rodent models of hemorrhagic shock that resuscitation with plasma is protective to the lungs compared with lactated Ringer's solution. As our long-term objective is to determine the molecular mechanisms that modulate plasma's protective effects in injured bleeding patients, we have used human plasma in a mouse model of hemorrhagic shock...
November 2016: Journal of Trauma and Acute Care Surgery
Tomasz Sozański, Alicja Z Kucharska, Andrzej Rapak, Dorota Szumny, Małgorzata Trocha, Anna Merwid-Ląd, Stanisław Dzimira, Tomasz Piasecki, Narcyz Piórecki, Jan Magdalan, Adam Szeląg
BACKGROUND AND AIMS: Cardiovascular benefits of fruits are attributed mainly to their (poly)phenolic constituents, especially anthocyanins. The main aim of our study is to compare effects of iridoids and anthocyanins from one fruit on diet-induced atherosclerosis. The cornelian cherry is a native or cultivated plant that grows in many European countries, used in cuisine and folk medicine. In our previous study, we showed its constituents and proved that oral administration of lyophilized fruits to hypercholesterolemic rabbits had preventive effects on atherosclerosis through the activation of PPARα expression...
October 5, 2016: Atherosclerosis
Eliza Kurek, Anna Ruszczyńska, Marcin Wojciechowski, Anna Łuciuk, Magdalena Michalska-Kacymirow, Ilona Motyl, Ewa Bulska
BACKGROUND: Selenium is an element of very great importance for the proper functioning of the human body, mainly due to its antioxidant properties. Selenium exhibits a preventive effect in the case of cardiovascular disease, the immune system, male infertility and inhibits the toxic action of other agents. Selenium is important for Hashimoto's disease. Intake of selenium in the diet slows the aging process. The biological and toxicological effects of selenium strongly depend on its chemical form...
2016: Roczniki Państwowego Zakładu Higieny
Zhanglong Peng, Shibani Pati, Magaili J Fontaine, Kelly Hall, Anthony V Herrera, Rosemary Ann Kozar
BACKGROUND: Clinical studies have demonstrated that the early and empiric use of plasma improves survival after hemorrhagic shock. We have demonstrated in rodent models of hemorrhagic shock that resuscitation with plasma is protective to the lungs compared to lactated Ringers. As our long term objective is to determine the molecular mechanisms that modulate plasma's protective effects in injured bleeding patients, we have used human plasma in a mouse model of hemorrhagic shock. The goal of the current experiments is to determine if there are significant adverse effects on lung injury when using human versus mouse plasma in an established murine model of hemorrhagic shock and laparotomy...
August 16, 2016: Journal of Trauma and Acute Care Surgery
Mai Thi Chi Tran, KienTrung Hoang, Ronda F Greaves
OBJECTIVES: This study aimed to evaluate the imprecision and bias data generated for 20 routine chemistry analytes against both the biological variation fitness for purpose (FFP) and Sigma metrics (SM) criteria. DESIGN AND METHOD: Twenty serum/plasma analytes were evaluated on the Beckman Coulter AU680. Third party commercial lyophilized internal quality control samples of human origin were used for day-to-day imprecision calculations. Commercial external quality assurance (EQA) samples were used to determine the systematic error between the test method result and the instrument group mean result from the EQA program for each analyte...
August 12, 2016: Clinical Biochemistry
B Radha Krishnan, Kenneth D James, Karen Polowy, B J Bryant, Anu Vaidya, Steve Smith, Christopher P Laudeman
: The echinocandins are an important class of antifungal agents. However, instability and, in some cases, lack of solubility have restricted their use to situations in which daily infusions are acceptable. CD101 is a novel echinocandin in development for topical and weekly i.v. administration that exhibits prolonged stability in plasma and aqueous solutions up to 40 °C. After incubation for 44 h in rat, dog, monkey and human plasma at 37 °C, the percent of CD101 remaining (91%, 79%, 94% and 93%, respectively) was consistently greater than that of anidulafungin (7%, 15%, 14% and 7%, respectively)...
August 10, 2016: Journal of Antibiotics
Chaoji Huangfu, Xiong Zhao, Maomin Lv, Junting Jia, Fengxuan Zhu, Rui Wang, Yuyuan Ma, Jingang Zhang
BACKGROUND: α2-Macroglobulin (α2-M) has a curative effect on radiation injury. Virus transmission through plasma derivatives is still not risk-free. Effect of dry heat on α2-M activity and virus inactivation by dry heat in a new manufacturing process of α2-M were studied. STUDY DESIGN AND METHODS: Effects of 100°C for 30 minutes, 80°C for 72 hours, and lyophilization on α2-M activity were detected, and stabilizing agents were optimized. Effect of a treatment at 100°C for 30 minutes has been tested on a range of viruses and characteristics change of α2-M was investigated...
September 2016: Transfusion
Justin J J Watson, Shibani Pati, Martin A Schreiber
Traumatic hemorrhage is the leading cause of preventable death after trauma. Early transfusion of plasma and balanced transfusion have been shown to optimize survival, mitigate the acute coagulopathy of trauma, and restore the endothelial glycocalyx. There are a myriad of plasma formulations available worldwide, including fresh frozen plasma, thawed plasma, liquid plasma, plasma frozen within 24 h, and lyophilized plasma (LP). Significant equipoise exists in the literature regarding the optimal plasma formulation...
November 2016: Shock
Günther Krumpl, Ivan Ulc, Michaela Trebs, Pavla Kadlecová, Juri Hodisch
BACKGROUND: To date, no data have been reported on the pharmacokinetic and pharmacodynamic properties of landiolol, a fast-acting cardioselective β1-adrenergic antagonist, in non-Asian subjects. The aim of this study was to compare two landiolol formulations in healthy Caucasian subjects. MATERIALS AND METHODS: We conducted a single-center, prospective, double-blinded, randomized study in two cross-over periods with 12 healthy subjects (7 women and 5 men) each receiving three doses (0...
September 20, 2016: European Journal of Pharmaceutical Sciences
Lisa N van der Vorm, Jan C M Hendriks, Coby M Laarakkers, Siem Klaver, Andrew E Armitage, Alison Bamberg, Anneke J Geurts-Moespot, Domenico Girelli, Matthias Herkert, Outi Itkonen, Robert J Konrad, Naohisa Tomosugi, Mark Westerman, Sukhvinder S Bansal, Natascia Campostrini, Hal Drakesmith, Marianne Fillet, Gordana Olbina, Sant-Rayn Pasricha, Kelly R Pitts, John H Sloan, Franco Tagliaro, Cas W Weykamp, Dorine W Swinkels
BACKGROUND: Absolute plasma hepcidin concentrations measured by various procedures differ substantially, complicating interpretation of results and rendering reference intervals method dependent. We investigated the degree of equivalence achievable by harmonization and the identification of a commutable secondary reference material to accomplish this goal. METHODS: We applied technical procedures to achieve harmonization developed by the Consortium for Harmonization of Clinical Laboratory Results...
July 2016: Clinical Chemistry
Rajesh Dabur, Ashwani Mittal
Fatty acid amides (FAAs) in alcoholism lead to liver diseases. These amides have been reported in plasma and in other organs of the body, while their detection or presence in the urine is still unknown. Therefore, the focus of the current study was to detect and analyze FAAs qualitatively in urine samples of alcoholics. Furthermore, the effects of Tinospora cordifolia (hepatoprotective medicinal plant) intervention on FAA levels in moderate alcoholics were also analyzed. In the study, asymptomatic chronic alcoholics (n = 22) without chronic liver disease and nonalcoholic healthy volunteers (n = 24) with a mean age of 39 ± 2...
May 2016: Alcohol
Anthony E Pusateri, Michael B Given, Martin A Schreiber, Philip C Spinella, Shibani Pati, Rosemary A Kozar, Abdul Khan, Joseph A Dacorta, Kevin R Kupferer, Nicolas Prat, Heather F Pidcoke, Victor W Macdonald, Wilbur W Malloy, Anne Sailliol, Andrew P Cap
The early transfusion of plasma is important to ensure optimal survival of patients with traumatic hemorrhage. In military and remote or austere civilian settings, it may be impossible to move patients to hospital facilities within the first few hours of injury. A dried plasma product with reduced logistical requirements is needed to enable plasma transfusion where medically needed, instead of only where freezers and other equipment are available. First developed in the 1930s, pooled lyophilized plasma was widely used by British and American forces in WWII and the Korean War...
April 2016: Transfusion
Jiaxing Wang, Jinhua Li, Shi Qian, Geyong Guo, Qiaojie Wang, Jin Tang, Hao Shen, Xuanyong Liu, Xianlong Zhang, Paul K Chu
Periprosthetic joint infection (PJI) is one of the formidable and recalcitrant complications after orthopedic surgery, and inhibiting biofilm formation on the implant surface is considered crucial to prophylaxis of PJI. However, it has recently been demonstrated that free-floating biofilm-like aggregates in the local body fluid and bacterial colonization on the implant and peri-implant tissues can coexist and are involved in the pathogenesis of PJI. An effective surface with both contact-killing and release-killing antimicrobial capabilities can potentially abate these concerns and minimize PJI caused by adherent/planktonic bacteria...
May 4, 2016: ACS Applied Materials & Interfaces
Liang Yang, Yating Shao, Hyo-Kyung Han
This study aimed to prepare the aminoclay-lipid hybrid composite to enhance the drug release and improve the oral bioavailability of poorly water-soluble fenofibrate. Antisolvent precipitation coupled with an immediate freeze-drying method was adopted to incorporate fenofibrate into aminoclay-lipid hybrid composite (ALC). The optimal composition of the ALC formulation was determined as the ratios of aminoclay to krill oil of 3:1 (w/w), krill oil to fenofibrate of 2:1 (w/w), and antisolvent to solvent of 6:4 (v/v)...
2016: International Journal of Nanomedicine
Gordana Juricic, Andrea Saracevic, Lara Milevoj Kopcinovic, Ana Bakliza, Ana-Maria Simundic
OBJECTIVES: Citrate buffer additive has been suggested to be of supreme performance in inhibiting glycolysis. However, there is little evidence in the literature regarding the comparability of glucose concentrations in liquid and lyophilized citrate buffer containing tubes. The aim of this study was to compare glucose concentrations in tubes containing liquid (Glucomedics) and lyophilized citrate buffer (Terumo VENOSAFE™ Glycemia) additive, measured immediately after centrifugation...
April 9, 2016: Clinical Biochemistry
Yuchao Chen, Jing Chen, Yi Cheng, Lihua Luo, Pinjing Zheng, Yidan Tong, Zhao Li
OBJECTIVES: In this study, an improved lyophilized PEGylated liposomal formulation of docetaxel (DOC) has been developed. METHODS: PEGylated docetaxel liposome (PL-DOC) was prepared by thin-film evaporation method and lyophilization. The effect of various components of the lipids and their compatibility with DOC on the entrapment efficiency (EE) of liposome was investigated. The lyophilized PL-DOC was characterized by morphology, particle size, zeta potential, EE, release in vitro and stability...
March 31, 2016: Journal of Liposome Research
Iman S Ahmed, Rania El-Hosary, Samia Shalaby, Marwa M Abd-Rabo, Dalia G Elkhateeb, Samia Nour
In this work lyophilized poly-ε-caprolactone nanoparticles (NPs) loaded with atorvastatin calcium (AC) were developed in an attempt to improve the in-vivo performance of AC following oral administration. The individual and combined effects of several formulation variables were previously investigated using step-wise full factorial designs in order to produce optimized AC-NPs with predetermined characteristics including particle size, drug loading capacity, drug release profile and physical stability. Four optimized formulations were further subjected in this work to lyophilization to promote their long-term physical stability and were fully characterized...
May 17, 2016: International Journal of Pharmaceutics
S J Thorpe, B Fox, G Sharp, J White, C Milkins
BACKGROUND AND OBJECTIVES: The aim of the study was to evaluate a lyophilized serum preparation, 14/300, for its suitability to serve as a World Health Organization (WHO) Reference Reagent to standardize and control haemagglutination titrations for anti-A and anti-B in serum and plasma, in an international collaborative study. MATERIALS AND METHODS: Serum preparation 14/300 and two plasma-based reserve preparations, 14/304 (high titre anti-A) and 14/208 (high titre anti-B), were titrated by 24 laboratories in 13 countries using direct (DRT) and indirect (IAT) haemagglutination techniques...
August 2016: Vox Sanguinis
Shelley H Jansky, Jacob Roble, David M Spooner
The natural history of epiphytic plant species has been extensively studied. However, little is known about the physiology and genetics of epiphytism. This is due to difficulties associated with growing epiphytic plants and the lack of tools for genomics studies and genetic manipulations. In this study, tubers were generated from 223 accessions of 42 wild potato Solanum species, including the epiphytic species S. morelliforme and its sister species S. clarum. Lyophilized samples were analyzed for 12 minerals using inductively coupled plasma optical emission spectrometry...
2016: Frontiers in Plant Science
Christopher S Crowe, Arhana Chattopadhyay, Rory McGoldrick, Grace Chiou, Hung Pham, James Chang
BACKGROUND: The authors have developed a tendon hydrogel that may be injected into the site of tendon injury to improve speed and strength of repair. The aim of this study was to compare the biological and physical properties of fresh, hydrated tendon hydrogel with its reconstituted lyophilized counterpart with the goal of increasing clinical feasibility. MATERIALS: Hydrogel was prepared from fresh human cadaveric flexor tendon. Fresh gel was compared to gel aliquots that were lyophilized and reconstituted with sterile deionized water...
March 2016: Plastic and Reconstructive Surgery
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