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in vivo hemolysis

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https://www.readbyqxmd.com/read/27888371/protective-effect-of-the-sulfated-agaran-isolated-from-the-red-seaweed-laurencia-aldingensis-against-toxic-effects-of-the-venom-of-the-snake-lachesis-muta
#1
Ana Cláudia Rodrigues da Silva, Luciana Garcia Ferreira, Maria Eugênia Rabello Duarte, Mutue Toyota Fujii, Eladio Flores Sanchez, Miguel Daniel Noseda, André Lopes Fuly
Snakebite is a serious occupational hazard affecting mainly rural populations of tropical and subtropical developing countries. Lachesis muta (Bushmaster) bites are extremely serious but are rarely reported in the literature. Bushmaster envenomings are characterized by intense local pain, edema, neurotoxicity, hypotension, local hemorrhage, and dramatic systemic alterations. Antivenom treatment has regularly been used for more than a century; however, it fails to neutralize local tissue damage and hemorrhage, leading to morbidity or disabilities in victims...
November 25, 2016: Marine Biotechnology
https://www.readbyqxmd.com/read/27886514/sugar-based-novel-niosomal-nanocarrier-system-for-enhanced-oral-bioavailability-of-levofloxacin
#2
Muhammad Imran, Muhammad Raza Shah, Farhat Ullah, Shafi Ullah, Abdelbary M A Elhissi, Waqas Nawaz, Farid Ahmad, Abdul Sadiq, Imdad Ali
CONTEXT: Vesicular systems have attracted great attention in drug delivery because of their amphiphilicity, biodegradability, non-toxicity and potential for increasing drug bioavailability. OBJECTIVE: A novel sugar-based double-tailed surfactant containing renewable block was synthesized for preparing niosomal vesicles that could be exploited for Levofloxacin encapsulation, aiming to increase its oral bioavailability. MATERIALS AND METHODS: The surfactant was characterized by (1)H NMR, mass spectroscopy and Fourier transform infrared spectroscopy (FT-IR)...
November 2016: Drug Delivery
https://www.readbyqxmd.com/read/27842837/synthesis-characterization-and-modification-of-gum-arabic-microgels-for-hemocompatibility-and-antimicrobial-studies
#3
Muhammad Farooq, Selin Sagbas, Mehtap Sahiner, Mohammad Siddiq, Mustafa Turk, Nahit Aktas, Nurettin Sahiner
Gum Arabic (GA) microgels were successfully prepared via reverse micellization method with high yield (78.5±5.0%) in 5-100μm size range using divinyl sulfone (DVS) as a crosslinker. The GA microgels were degraded hydrolytically 22.8±3.5% at pH 1 in 20days, whereas no degradation was observed at pH 7.4 and pH 9 at 37°C. By using diethylenetriamine (DETA), and taurine (TA) as chemical modifying agents, GA microgels were chemically modified as GA-DETA and GA-TA, and the zeta potential values of 5.2±4.1 and -24...
January 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27822958/double-tailed-acyl-glycoside-niosomal-nanocarrier-for-enhanced-oral-bioavailability-of-cefixime
#4
Muhammad Imran, Muhammad Raza Shah, Farhat Ullah, Shafi Ullah, Abdul Sadiq, Imdad Ali, Farid Ahmed, Waqas Nawaz
Novel, safe, efficient, and cost effective surfactants from renewable resources has attracted attention for enhancing solubility and bioavailability of hydrophobic dugs. We report the synthesis, characterization, and biocompatibility of a novel non-ionic acyl glycoside double-tailed surfactant for niosomal drug delivery system. Structure of the surfactant was confirmed by (1)H NMR and mass spectroscopy. Applications of surfactant in niosomal drug delivery were explored using Cefixime as model. The shape, size, and polydispersity index (PDI) of drug loaded vesicles were investigated with atomic force microscope (AFM) and dynamic light scattering (DLS)...
November 8, 2016: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/27822040/raspberry-like-poly-%C3%AE-glutamic-acid-hydrogel-particles-for-ph-dependent-cell-membrane-passage-and-controlled-cytosolic-delivery-of-antitumor-drugs
#5
Sun-Hee Cho, Ji Hyeon Hong, Young-Woock Noh, Eunji Lee, Chang-Soo Lee, Yong Taik Lim
In this research, we synthesized bioderived poly(amino acid) hydrogel particles that showed pH-dependent membrane-disrupting properties and controlled cytosolic delivery of antitumor drugs. Poly(γ-glutamic acid) (γ-PGA) that has been produced extensively using bacteria, especially those of Bacillus subtilis species, was modified with cholesterol (γ-PGA/Chol), and the γ-PGA/Chol conjugates were used to form polymeric nanoparticles the size of 21.0±1.1 nm in aqueous solution. When the polymeric nanoparticles were mixed with doxorubicin (Dox), raspberry-like hydrogel particles (RBHPs) were formed by the electrostatic interaction between the cationically charged Dox and the anionically charged nanoparticles...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27821769/protein-aggregation-as-a-cellular-response-to-oxidative-stress-induced-by-heme-and-iron
#6
Luiz R C Vasconcellos, Fabianno F Dutra, Mariana S Siqueira, Heitor A Paula-Neto, Jennifer Dahan, Ellen Kiarely, Leticia A M Carneiro, Marcelo T Bozza, Leonardo H Travassos
Hemolytic diseases include a variety of conditions with diverse etiologies in which red blood cells are destroyed and large amounts of hemeproteins are released. Heme has been described as a potent proinflammatory molecule that is able to induce multiple innate immune responses, such as those triggered by TLR4 and the NLRP3 inflammasome, as well as necroptosis in macrophages. The mechanisms by which eukaryotic cells respond to the toxic effects induced by heme to maintain homeostasis are not fully understood, however...
November 22, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27807330/-toxicity-studies-in-vivo-and-in-vitro-for-sheep-skin-%C3%A2-acellular-dermal-matrix
#7
Jun Zhang, Xiaoyuan Huang, Lingfeng Wang
To detect the toxic reaction degree for sheep acellular dermal matrix (ADM) in vivo or vitro by using hemolytic, pyrogen and cell-cytotoxic reaction experiments, respectively.
 Methods: Leach liquor of cross-linked and non-cross-linked sheep ADMs were set for cross-linked group and non-cross-linked group, respectively, with a positive control group (10 mL sterile water for injection in test tube) and a negative control group (10 mL 0.9% sodium chloride solution in test tube). The supernatants were obtained from each group and were measured for the absorbance...
October 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/27796140/in-vitro-and-in-vivo-evaluation-of-paclitaxel-lapatinib-loaded-f127-pluronic-micelles
#8
Pouya Dehghankelishadi, Ebrahim Saadat, Fatemeh Ravar, Maliheh Safavi, Mahboobeh Pordeli, Mehdi Gholami, Farid A Dorkoosh
The aim of this study was to evaluate the in vitro and in vivo efficacy of Paclitaxel-Lapatinib loaded Pluronic micelles. Lapatinib and pluronic sensitize the cancerous cells to Paclitaxel via efflux pump inhibition. In addition, pluronic polymers can trigger intrinsic apoptosis pathways. Furthermore, micellar system can passively target the chemotherapeutic agents by enhanced permeability and retention effect. The paclitaxel-lapatinib loaded micelles were characterized in means of encapsulation efficacy and size...
October 31, 2016: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/27770914/corrosion-resistance-and-biocompatibility-of-magnesium-alloy-modified-by-alkali-heating-treatment-followed-by-the-immobilization-of-poly-ethylene-glycol-fibronectin-and-heparin
#9
Changjiang Pan, Youdong Hu, Yu Hou, Tao Liu, Yuebin Lin, Wei Ye, Yanhua Hou, Tao Gong
In recent years, magnesium alloys are attracting more and more attention as a kind of biodegradable metallic biomaterials, however, their uncontrollable biodegradation speed in vivo and the limited surface biocompatibility hinder their clinical applications. In the present study, with the aim of improving the corrosion resistance and biocompatibility, the magnesium alloy (AZ31B) surface was modified by alkali heating treatment followed by the self-assembly of 3-aminopropyltrimethoxysilane (APTMS). Subsequently, poly (ethylene glycol) (PEG) and fibronectin or fibronectin/heparin complex were sequentially immobilized on the modified surface...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27752974/hydrogels-based-on-poly-ethylene-glycol-as-scaffolds-for-tissue-engineering-application-biocompatibility-assessment-and-effect-of-the-sterilization-process
#10
Alondra Escudero-Castellanos, Blanca E Ocampo-García, Ma Victoria Domínguez-García, Jaime Flores-Estrada, Miriam V Flores-Merino
Hydrogels are suitable materials to promote cell proliferation and tissue support because of their hydrophilic nature, porous structure and sticky properties. However, hydrogel synthesis involves the addition of additives that can increase the risk of inducing cytotoxicity. Sterilization is a critical process for hydrogel clinical use as a proper scaffold for tissue engineering. In this study, poly(ethylene glycol) (PEG), poly(ethylene glycol)-chitosan (PEG-CH) and multi-arm PEG hydrogels were synthesized by free radical polymerization and sterilized by gamma irradiation or disinfected using 70 % ethanol...
December 2016: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27727185/protective-effect-of-the-plant-extracts-of-erythroxylum-sp-against-toxic-effects-induced-by-the-venom-of-lachesis-muta-snake
#11
Eduardo Coriolano de Oliveira, Rodrigo Alves Soares Cruz, Nayanna de Mello Amorim, Marcelo Guerra Santos, Luiz Carlos Simas Pereira Junior, Eladio Oswaldo Flores Sanchez, Caio Pinho Fernandes, Rafael Garrett, Leandro Machado Rocha, André Lopes Fuly
Snake venoms are composed of a complex mixture of active proteins that induce toxic effects, such as edema, hemorrhage, and death. Lachesis muta has the highest lethality indices in Brazil. In most cases, antivenom fails to neutralize local effects, leading to disabilities in victims. Thus, alternative treatments are under investigation, and plant extracts are promising candidates. The objective of this work was to investigate the ability of crude extracts, fractions, or isolated products of Erythroxylum ovalifolium and Erythroxylum subsessile to neutralize some toxic effects of L...
October 11, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27725938/optimized-collection-protocol-for-plasma-microrna-measurement-in-patients-with-cardiovascular-disease
#12
Chi-Sheng Wu, Fen-Chiung Lin, Shu-Jen Chen, Yung-Lung Chen, Wen-Jung Chung, Cheng-I Cheng
Background. Various microRNAs (miRNAs) are used as markers of acute coronary syndrome, in which heparinization is considered mandatory therapy. Nevertheless, a standard method of handling plasma samples has not been proposed, and the effects of heparin treatment on miRNA detection are rarely discussed. Materials and Method. This study used quantitative polymerase chain reaction (qPCR) analysis to investigate how storage temperature, standby time, hemolysis, and heparin treatment affect miRNA measurement in plasma samples from 25 patients undergoing cardiac catheterization...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27718524/antisense-locked-nucleic-acids-targeting-agra-inhibit-quorum-sensing-and-pathogenesis-of-community-associated-methicillin-resistant-staphylococcus-aureus
#13
Fei Da, Lin Yao, Zhijie Su, Zhen Hou, Zhi Li, Xiaoyan Xue, Jingru Meng, Xiaoxing Luo
AIM: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is commonly associated with non-nosocomial skin and soft tissue infections (SSTIs) due to its virulence, which is mainly controlled by the accessory gene regulator (agr) quorum sensing (QS) system. In this study (KFF)3 K peptide-conjugated Locked Nucleic Acids (PLNAs) targeting agrA mRNA were developed to inhibit agr activity and arrest the pathogenicity of CA-MRSA. METHODS AND RESULTS: Two PLNAs were designed, and synthesized, after predicting the secondary structure of agrA mRNA...
October 8, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27706881/efficient-and-non-toxic-gene-delivery-by-anionic-lipoplexes-based-on-polyprenyl-ammonium-salts-and-their-effects-on-cell-physiology
#14
Monika Rak, Anna Ochałek, Ewa Bielecka, Joanna Latasiewicz, Katarzyna Gawarecka, Jolanta Sroka, Jarosław Czyż, Katarzyna Piwowarczyk, Marek Masnyk, Marek Chmielewski, Tadeusz Chojnacki, Ewa Swiezewska, Zbigniew Madeja
BACKGROUND: One of the major challenges limiting development of gene therapy is absence of efficient and safe gene carriers. Among the non-viral gene delivery methods, lipofection is considered as one of the most promising. In this study a set of cationic polyprenyl derivatives (trimethylpolyprenylammonium iodides - PTAI) with different lengths of polyprenyl chains (from 7, 8 and 11 to 15 isoprene units) was suggested as a component of efficient DNA vehicles. METHODS: Optimization studies were conducted for PTAI in combination with co-lipid DOPE (dioleoylphosphatidylethanolamine) on DU145 human prostate cancer cells using: size and zeta potential measurements, confocal microscopy, fluorescein diacetate/ethidium bromide test, cell counting, time-lapse monitoring of cell movement, gap junctional intracellular coupling analysis, antimicrobial activity assay, red blood cell hemolysis test...
October 5, 2016: Journal of Gene Medicine
https://www.readbyqxmd.com/read/27693741/apigenin-protects-mice-from-pneumococcal-pneumonia-by-inhibiting-the-cytolytic-activity-of-pneumolysin
#15
Meng Song, Li Li, Meng Li, Yonghong Cha, Xuming Deng, Jianfeng Wang
Streptococcus pneumoniae is an important human pathogenic bacterium that can cause various life-threatening infections. Pneumolysin (PLY), the pore-forming toxin that forms large pores in the cell membrane, is a key virulence factor secreted by S. pneumoniae that penetrates the physical defenses of the host and plays an important role in the pathogenesis of pneumococcal diseases, such as pneumonia, meningitis, bacteremia and otitis media. This study showed that apigenin, one of the bioflavonoids widely found in herbs, inhibits PLY-induced hemolysis by inhibiting the oligomerization of PLY and has no anti-S...
September 28, 2016: Fitoterapia
https://www.readbyqxmd.com/read/27669368/retrospective-evaluation-of-the-effect-of-red-blood-cell-product-age-on-occurrence-of-acute-transfusion-related-complications-in-dogs-210-cases-2010-2012
#16
Christina H Maglaras, Amie Koenig, Deanna L Bedard, Benjamin M Brainard
OBJECTIVE: To determine whether red blood cell (RBC) product age influences the occurrence of acute transfusion-related complications and mortality in dogs. The hypothesis was that acute transfusion-related complications and mortality would increase with age of product. DESIGN: Retrospective study (2010-2012). SETTING: University teaching hospital. ANIMALS: Two hundred and ten clinical canine patients. INTERVENTIONS: None...
September 26, 2016: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/27652265/preparation-and-characterization-of-novel-perfluorooctyl-bromide-nanoparticle-as-ultrasound-contrast-agent-via-layer-by-layer-self-assembly-for-folate-receptor-mediated-tumor-imaging
#17
Yue Hu, Yong Wang, Jianshuai Jiang, Baosan Han, Shengmin Zhang, Keshi Li, ShuXiong Ge, Yahui Liu
A folate-polyethylene glycol-chitosan derivative was synthesized and its structure was characterized. An optimal perfluorooctyl bromide nanocore template was obtained via utilizing the ultrasonic emulsification method combining with orthogonal design. The targeted nanoparticles containing targeted shell of folate-polyethylene glycol-chitosan derivative and perfluorooctyl bromide nanocore template of ultrasound imaging were prepared successfully by exploiting layer-by-layer self-assembly as contrast agent for ultrasound...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27650695/proteomics-and-antivenomics-of-papuan-black-snake-pseudechis-papuanus-venom-with-analysis-of-its-toxicological-profile-and-the-preclinical-efficacy-of-australian-antivenoms
#18
Davinia Pla, Benjamin W Bande, Ronelle E Welton, Owen K Paiva, Libia Sanz, Álvaro Segura, Christine E Wright, Juan J Calvete, José María Gutiérrez, David J Williams
: The Papuan black snake (Pseudechis papuanus Serpentes: Elapidae) is endemic to Papua New Guinea, Indonesian Papua and Australia's Torres Strait Islands. We have investigated the biological activity and proteomic composition of its venom. The P. papuanus venom proteome is dominated by a variety (n≥18) of PLA2s, which together account for ~90% of the venom proteins, and a set of low relative abundance proteins, including a short-neurotoxic 3FTx (3.1%), 3-4 PIII-SVMPs (2.8%), 3 cysteine-rich secretory proteins (CRISP; 2...
September 17, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27639705/antifungal-and-immunomodulatory-activity-of-a-novel-cochleate-for-amphotericin-b-delivery-against-sporothrix-schenckii
#19
A Batista-Duharte, M Lastre, B Romeu, D L Portuondo, D Téllez-Martínez, F A Manente, O Pérez, I Z Carlos
INTRODUCTION: Sporotrichosis is an emergent subcutaneous mycoses caused by species of the Sporothrix schenckii complex. Amphotericin B (AmB) remains the main antifungal drug for the treatment of systemic infections, but its use is limited by toxicity reasons. AFCo3 is a novel cochleate containing detoxified LPS, which exhibits drug delivery and immunomodulating properties. Here, AFCo3 was used as the vehicle for AmB to evaluate the immunomodulatory and antifungal efficacy against S. schenckii in vitro and in vivo...
September 15, 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27632696/in-vitro-and-in-vivo-evaluation-of-redox-responsive-sorafenib-carrier-nanomicelles-synthesized-from-poly-acryic-acid-cystamine-hydrochloride-d-%C3%AE-tocopherol-succinate
#20
Yu Liu, Jia Yang, Xin Wang, Ju Liu, Zhaobo Wang, Hongsheng Liu, Lijiang Chen
In this study, a controlled drug release system based on redox-responsive nanomicelles for drug delivery was described. The system was constructed by linking poly (acryic acid) (PAA) with D-α-tocopherol succinate (VES) via a disulfide bond linker (ss). This amphiphilic polymer (PAAssVES) was synthesized by coupling reaction and its chemical structure was confirmed by FT-IR and (1)HNMR analyses. PAAssVES was found to self-assemble into nanomicelles with diameter of about 130 nm, and a critical micelle concentration of about 6...
December 2016: Journal of Biomaterials Science. Polymer Edition
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