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in vivo hemolysis

T C Moraes Moreira Carraro, C Altmeyer, N Maissar Khalil, R Mara Mainardes
Amphotericin B (AmB) is widely applied in treatment of systemic fungal infections. However, the emergence of severe adverse effects, such as nephrotoxicity, hepatotoxicity and hemolytic anemia, can limit its clinical use. Poly(lactide-co-glycolide) (PLGA) or poly(lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG) blend nanoparticles containing AmB were developed with the aim to decrease AmB toxicity and propose the oral route for AmB delivery. Nanoparticles were characterized by particle size, polydispersity index, Fourier transform infrared spectroscopy, differential scanning calorimetry and X-ray diffraction analyses...
August 7, 2017: Journal de Mycologie Médicale
Aránzazu Sáenz, Natalia Ortiz, Bruno Lomonte, Alexandra Rucavado, Cecilia Díaz
Pterois volitans/miles lionfish (adult and juvenile) dorsal spines and caudal fin extracts were compared in their general composition, enzymatic activities and hemolytic and cytotoxic effects on bovine aortic endothelial cells and murine myoblasts, to distinguish between the activities present in the venom and epidermal mucus. Intradermal and intramuscular injections were also administered in mice to determine in vivo effects. This work shows that crude venom of Caribbean species of lionfish, present in dorsal spines, induces several in vitro effects including hemolysis, weak cytotoxicity, proteolytic and hyaluronidase activities, whereas in vivo, it is not hemorrhagic nor myotoxic, but causes edema, plasma extravasation and a thrombotic-associated lesion on the skin...
August 5, 2017: Toxicon: Official Journal of the International Society on Toxinology
Pankaj K Singh, Vivek K Pawar, Anil K Jaiswal, Yuvraj Singh, Cheruvu Hanumanth Srikanth, Mohini Chaurasia, Himangsu K Bora, Kavit Raval, Jaya Gopal Meher, Jiaur R Gayen, Anuradha Dube, Manish K Chourasia
The goal of study was to develop micellar formulation of Amphotericin B (AmB) to improve its antileishmanial efficacy. AmB loaded pluronic F127 (PF 127) micelles were developed and coated with chitosan (Cs-PF-AmB-M) to accord immunoadjuvant and macrophage targeting properties. Hemolysis and cytotoxicity studies demonstrated that Cs-PF-AmB-M was 7.93 fold (at 20μg/ml AmB concentration) and 9.35 fold less hemolytic and cytotoxic, respectively in comparison to AmB suspension. Flow cytometry studies indicated that Cs-PF-FITC-M was 21...
August 2, 2017: International Journal of Biological Macromolecules
Harald Unterweger, Christina Janko, Marc Schwarz, László Dézsi, Rudolf Urbanics, Jasmin Matuszak, Erik Őrfi, Tamás Fülöp, Tobias Bäuerle, János Szebeni, Clément Journé, Aldo R Boccaccini, Christoph Alexiou, Stefan Lyer, Iwona Cicha
Iron oxide-based contrast agents have been in clinical use for magnetic resonance imaging (MRI) of lymph nodes, liver, intestines, and the cardiovascular system. Superparamagnetic iron oxide nanoparticles (SPIONs) have high potential as a contrast agent for MRI, but no intravenous iron oxide-containing agents are currently approved for clinical imaging. The aim of our work was to analyze the hemocompatibility and immuno-safety of a new type of dextran-coated SPIONs (SPIONdex) and to characterize these nanoparticles with ultra-high-field MRI...
2017: International Journal of Nanomedicine
Lu Li, Xing Cao, Xin Shen, Shitao Yu, Feng Su, Shiwei Liu, Fusheng Liu, Congxia Xie
Biofilm with strong tensile strength is a topic item in the area of tissue engineering, medicine engineering, etc. Here we introduced an alkyl chitosan film with strong tensile strength and its possibility for an absorbable anticoagulation material in vivo was tested in the series of blood test, such as dynamic coagulation time, plasma recalcification time and hemolysis. Alkyl chitosan film was a better biomaterial than traditional chitosan film in the anticoagulation, tissue compatibility and cell compatibility...
August 2, 2017: Journal of Biomedical Materials Research. Part A
Sławomir Jaworski, Mateusz Hinzmann, Ewa Sawosz, Marta Grodzik, Marta Kutwin, Mateusz Wierzbicki, Barbara Strojny, Krishna P Vadalasetty, Ludwika Lipińska, André Chwalibog
With the rapid development of graphene synthesis and functionalization approaches, graphene and its related derivatives have shown great potential in many applications in material science, including biomedical applications. Several in vitro and in vivo studies clearly showed no definitive risks, while others have indicated that graphene might become health hazards. In this study, we explore the biocompatibility of graphene-related materials with chicken embryo red blood cells (RBC). The hemolysis assay was employed to evaluate the in vitro blood compatibility of reduced graphene, graphene oxide, and reduced graphene oxide, because these materials have recently been used for biomedical applications, including injectable graphene-related particles...
July 28, 2017: Environmental Science and Pollution Research International
Chris H H Chan, Ina Laura Pieper, Christian R Robinson, Yasmin Friedmann, Venkateswarlu Kanamarlapudi, Catherine A Thornton
The common complications in heart failure patients with implanted ventricular assist devices (VADs) include hemolysis, thrombosis, and bleeding. These are linked to shear stress-induced trauma to erythrocytes, platelets, and von Willebrand factor (vWF). Novel device designs are being developed to reduce the blood trauma, which will need to undergo in vitro and in vivo preclinical testing in large animal models such as cattle, sheep, and pig. To fully understand the impact of device design and enable translation of preclinical results, it is important to identify any potential species-specific differences in the VAD-associated common complications...
July 26, 2017: Artificial Organs
Wei Shan, Yi Cui, Min Liu, Lei Wu, Yucheng Xiang, Quan Guo, Zhirong Zhang, Yuan Huang
Development of nanoparticle (NP) to simultaneously overcome the diffusion and absorption barrier has drawn much attention. Our group recently demonstrated that the virus mimicking dilauroylphosphatidylcholine functionalized NP (DLPC-NP) could rapidly penetrate across mucus layer and subsequently enter epithelia effectively, and exhibited high potential for oral drug delivery. However, the safety of the NP has provoked some scientific concerns, which debates from their properties that circuiting the protections of mucus and exhibiting strong interaction with cell membrane...
July 22, 2017: International Journal of Pharmaceutics
J W Kuhbier, V Coger, J Mueller, C Liebsch, F Schlottmann, V Bucan, P M Vogt, S Strauss
Spider silk became one of the most-researched biomaterials in the last years due to its unique mechanical strength and most favourable chemical composition for tissue engineering purposes. However, standardized analysis of cytocompatibility is missing. Therefore, the aim of this study was to investigate hemolysis, cytotoxicity of native spider silk as well as influences on the cell culture medium. Changes of cell culture medium composition, osmolarity as well as glucose and lactate content were determined via ELISA measurement...
August 2017: Journal of Materials Science. Materials in Medicine
L J Yan, P Chang, R H Tian
Twinky Star colored compomer produced by VOCO GmbH is a new type of filling material designed with seven different colors. Previous studies have shown that using the colored filling material can induce children’s curiosity and increase the acceptance for dental treatment. However, few studies have been conducted to evaluate it. Hence, the present study was designed to assess the mechanical and adhesive properties of this new compomer, examine its biological safety, and evaluate the effects of its clinical application on children’s adherence to dental treatment and the subsequent therapeutic outcomes...
April 2017: Journal of Biological Regulators and Homeostatic Agents
Edgardo Rojas-Mancilla, Alexis Oyarce, Viviana Verdugo, Cesar Morales-Verdejo, Cesar Echeverria, Felipe Velásquez, Jonas Chnaiderman, Fernando Valiente-Echeverría, Rodrigo Ramirez-Tagle
The molybdenum cluster [Mo₆Cl14](2-) is a fluorescent component with potential for use in cell labelling and pharmacology. Biological safety and antiviral properties of the cluster are as yet unknown. Here, we show the effect of acute exposition of human cells and red blood cells to the molybdenum cluster and its interaction with proteins and antiviral activity in vitro. We measured cell viability of HepG2 and EA.hy926 cell lines exposed to increasing concentrations of the cluster (0.1 to 250 µM), by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay...
July 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Hong-Jyuan Jian, Ren-Siang Wu, Tzu-Yu Lin, Yu-Jia Li, Han-Jia Lin, Scott G Harroun, Jui-Yang Lai, Chih-Ching Huang
We have developed a one-step method to synthesize carbon quantum dots (CQDPAs) from biogenic polyamines (PAs) as an antibacterial agent for topical treatment of bacterial keratitis (BK). CQDs synthesized by direct pyrolysis of spermidine (Spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from putrescine and spermine. We demonstrate that CQDs obtained from Spds (CQDSpds) possess effective antibacterial activities against non-multidrug-resistant Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica serovar Enteritidis bacteria and also against the multidrug-resistant bacteria, methicillin-resistant S...
July 25, 2017: ACS Nano
Ahmed O Elzoghby, Shaimaa K Mostafa, Maged W Helmy, Maha A ElDemellawy, Salah A Sheweita
Despite several reports have revealed the beneficial effect of co-administration of COX-2 inhibitors with aromatase inhibitors in managing postmenopausal breast cancer; no nanocarriers for such combined delivery have been developed till now. Therefore, protamine nanocapsules (PMN-NCs) have been developed to co-deliver letrozole (LTZ) that inhibits aromatase-mediated estrogen biosynthesis and celecoxib (CXB) that synergistically inhibits aromatase expression. Inspired by the CD44-mediated tumor targeting ability of hyaluronate (HA), we developed HA-coated PMN-NCs (HA-NCs) via electrostatic layer-by-layer assembly...
June 27, 2017: International Journal of Pharmaceutics
Roxana Cristina Popescu, Ecaterina Andronescu, Bogdan Ștefan Vasile, Roxana Truşcă, Adina Boldeiu, Laurențiu Mogoantă, George Dan Mogoșanu, Mihaela Temelie, Mihai Radu, Alexandru Mihai Grumezescu, Diana Savu
Nanotechnology has been successfully used for the fabrication of targeted anti-cancer drug carriers. This study aimed to obtain Fe₃O₄ nanoparticles functionalized with Gemcitabine to improve the cytotoxic effects of the chemotherapeutic substance on cancer cells. The (un) functionalized magnetite nanoparticles were synthesized using a modified co-precipitation method. The nanoconjugate characterization was performed by XRD, SEM, SAED and HRTEM; the functionalizing of magnetite with anti-tumor substances has been highlighted through TGA...
June 28, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Arehalli S Manjappa, Popat S Kumbhar, Prajakta S Khopade, Ajit B Patil, John I Disouza
Docetaxel (DTX) has been used to treat several types of cancers, but it has provided pharmaceutical challenges due to its poor water solubility and toxicities associated with the co-solvents (tween-80 and ethanol). Nanopolymer therapeutics can be engineered to deliver anticancer agent specifically to cancer cells, thereby leaving normal healthy cells unaffected by toxic drugs such as DTX. The objective of the present study was to synthesize the polyacrylic acid (PAA)-DTX conjugate (PAADC) and preparation of nanopolymer therapeutics such as PAADC/DSPE-mPEG2000 mixed micelles (PAADC-DP MMs)...
June 21, 2017: Current Drug Delivery
Xuefang Ding, Yujie Su, Cheng Wang, Fangrong Zhang, Kerong Chen, Yu Wang, Min Li, Wei Wang
Single-walled carbon nanotubes (SWNTs) with unique physicochemical properties have exhibited promising biomedical applications as drug and gene carriers. In this study, polyethylenimine (PEI)-modified SWNT conjugates linked with candesartan (CD) were developed to deliver vascular endothelial growth factor (VEGF)-targeted siRNA (siVEGF) for the synergistic and targeted treatment of tumor angiogenesis. The characterization results revealed that SWNT-PEI-CD conjugates were successfully synthesized and exhibited desirable dispersibility and superior stability...
July 19, 2017: ACS Applied Materials & Interfaces
Shweta Dumoga, Yogesh Rai, Anant Narayan Bhatt, Anjani Kumar Tiwari, Surendra Singh, Anil K Mishra, Dipti Kakkar
Polymer-based nanoparticles have proven to be viable carriers of therapeutic agents. In this study, we have developed nanoparticles (NPs) from polypeptide-polyethylene glycol based triblock and diblock copolymers. The synthesized block copolymers poly(ethylene glycol)-b-poly(glutamic acid)-b-poly(ethylene glycol) (GEG) and poly(ethylene glycol)-b-poly(glutamic acid) (EG) conjugated with folic acid for targeting specificity (EGFA) have been used to encapsulate methotrexate (MTX) to form M-GEG and M-EGFA NPs aimed at passive and active targeting of cervical carcinoma...
July 12, 2017: ACS Applied Materials & Interfaces
Shansong Mu, Guowei Li, Yuanyuan Liang, Ting Wu, Dong Ma
As a promising drug carrier, graphene oxide (GO) has been studied widely in drug delivery due to its excellent loading ability to aromatic drugs. However, its biocompatibility, such as stability and blood compatibility, is now the biggest obstacle for its further application. Herein, the hyperbranched polyglycerol (HPG)-modified GO was prepared through the anionic ring-opening polymerization using GO as the initiator directly, and then the doxorubicin hydrochloride was loaded and showed obvious cytotoxicity to tumor cells...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Hyeongseop Keum, Jin Yong Kim, Byeongjun Yu, Seung Jung Yu, Jinjoo Kim, Hyungsu Jeon, Dong Yun Lee, Sung Gap Im, Sangyong Jon
As reports of multidrug resistant pathogens have increased, patients with implanted medical catheters increasingly need alternative solutions to antibiotic treatments. As most catheter-related infections are directly associated with biofilm formation on the catheter surface, which, once formed, is difficult to eliminate, a promising approach to biofilm prevention involves inhibiting the initial adhesion of bacteria to the surface. In this study, we report an amphiphilic, antifouling polymer, poly(DMA-mPEGMA-AA) that can facilely coat the surfaces of commercially available catheter materials in water and prevent bacterial adhesion to and subsequent colonization of the surface, giving rise to an antibiofilm surface...
June 14, 2017: ACS Applied Materials & Interfaces
William J Savage, John R Burns, Jason Fiering
BACKGROUND: Acoustic cell separation uses ultrasound waves to separate cells from plasma to perform plasmapheresis. Although the fundamental process has been studied for decades, no acoustic cell separation has been studied in a disposable plastic format suitable for clinical applications. STUDY DESIGN AND METHODS: We developed a disposable, rectangular, polystyrene microchannel acoustic cell-separation system and applied acoustic energy relevant for plasmapheresis...
May 28, 2017: Transfusion
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