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in vivo hemolysis

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https://www.readbyqxmd.com/read/29684116/biofilm-forming-ability-and-infection-potential-of-pseudomonas-aeruginosa-strains-isolated-from-animals-and-humans
#1
Dusan Milivojevic, Neven Šumonja, Strahinja Medic, Aleksandar Pavic, Ivana Moric, Branka Vasiljevic, Lidija Senerovic, Jasmina Nikodinovic-Runic
Pseudomonas aeruginosa, has been amongst top 10 'superbugs' worldwide and is causing infections with poor outcomes in both humans and animals. From 202 P. aeruginosa isolates (n = 121 animal and n = 81 human) 40 were selected on the basis of biofilm-forming ability and were comparatively characterized in terms of virulence determinants to the type strain P. aeruginosa PAO1. Biofilm formation, pyocyanin and hemolysins production and bacterial motility patterns were compared to the ability to kill human cell line A549 in vitro...
April 19, 2018: Pathogens and Disease
https://www.readbyqxmd.com/read/29667205/application-of-heparin-collagen-redv-selective-active-interface-on-eptfe-films-to-enhance-endothelialization-and-anticoagulation
#2
Yaping Shan, Bing Jia, Ming Ye, Hua Shen, Weicheng Chen, Huifeng Zhang
Expanded polytetrafluoroethylene (ePTFE) prosthetic valves have been widely used in clinical applications in Asian countries. However, these valves still have limits with regard to thrombosis, neointimal hyperplasia, restenosis, and valvar vegetation. The achievement of in situ endothelialization on implant materials is a promising way to overcome those limits. Here, heparin/collagen multilayers were fabricated on ePTFE films via a layer-by-layer (LBL) self-assembly technique, and then, the endothelial cell (EC) adhesive peptide sequence Arg-Glu-Asp-Val (REDV) was immobilized on the multilayers...
April 18, 2018: Artificial Organs
https://www.readbyqxmd.com/read/29655958/a-second-derivate-fitting-algorithm-for-the-quantification-of-free-hemoglobin-in-human-plasma
#3
Michael Paal, Alexander Lang, Georg Hennig, Marie-Luise Buchholtz, Ronald Sroka, Michael Vogeser
BACKGROUND: Assessment of hemolysis in vivo is becoming increasingly relevant in critical care. Current methods (Harboe, 1959) for quantifying the free hemoglobin (fHb) content produce unsatisfactory results in case of hyperbilirubinemia, a frequent condition in patients at risk for intravascular hemolysis. METHODS: A novel evaluation method based on second-derivative fitting to quantify fHb content was developed. The method uses spectrophotometric data from 350 to 650 nm recorded with standard instruments as input...
April 12, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29611201/a-practical-toxicity-bioassay-for-vicine-convicine-levels-in-faba-bean-vicia-faba
#4
Fitsum Getachew, Albert Vandenberg, Judit Smits
BACKGROUND: Faba bean (Vicia faba) vicine/convicine (V-C) aglycones (divicine/isouramil) provoke an acute hemolytic anemia called favism in individuals with a glucose-6-phosphate dehydrogenase (G6PD) enzyme defect in their red blood cells. Geneticists/plant breeders are working with faba bean to decrease V-C levels to improve public acceptance of this high protein pulse crop. Here, we present a fast and simple ex-vivo in vitro bioassay for V-C toxicity testing of faba bean or faba bean food products...
April 2, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29610666/revisiting-the-putative-role-of-heme-as-a-trigger-of-inflammation
#5
Florence Vallelian, Christian A Schaer, Jeremy W Deuel, Giada Ingoglia, Rok Humar, Paul W Buehler, Dominik J Schaer
Activation of the innate immune system by free heme has been proposed as one of the principal consequences of cell-free hemoglobin (Hb) exposure. Nonetheless, in the absence of infection, heme exposures within a hematoma, during hemolysis, or upon systemic administration of Hb (eg, as a Hb-based oxygen carrier) are typically not accompanied by uncontrolled inflammation, challenging the assumption that heme is a major proinflammatory mediator in vivo. Because of its hydrophobic nature, heme liberated from oxidized hemoglobin is rapidly transferred to alternative protein-binding sites (eg, albumin) or to hydrophobic lipid compartments minimizing protein-free heme under in vivo equilibrium conditions...
April 2018: Pharmacology Research & Perspectives
https://www.readbyqxmd.com/read/29602078/increasing-the-anticancer-performance-of-bufalin-buf-by-introducing-an-endosome-escaping-polymer-and-tumor-targeting-peptide-in-the-design-of-a-polymeric-prodrug
#6
Xiao-Jing Shi, Yan-Yan Qiu, Hui Yu, Cheng Liu, Yu-Xia Yuan, Pei-Hao Yin, Tao Liu
A well-defined multifunctional brush-type polymeric prodrug covalently linked with an anticancer drug (bufalin, BUF), a tumor-targeting peptide (RGD), and an endosome-escaping polymer, poly(N,N-diethylaminoethyl methacrylate-co-butyl methacrylate (P(DEA-co-BMA)), was developed. Its anticancer performance against colon cancer was investigated in vitro and in vivo. Reversible addition-fragmentation transfer (RAFT) polymerization of oligo(ethylene glycol) monomethyl ether methacrylate (OEGMA), 2-((3-(tert-butoxy)-3-oxopropyl)thio)ethyl methacrylate (BSTMA), and 2-(2-bromoisobutyryloxy)ethylmethacrylate (BIEM) afforded the multifunctional random copolymer, P(OEGMA-co-BSTMA-co-BIEM), in which hydrophilic POEGMA can stabilize nanoparticles in water, PBSTMA can be converted into carboxyl groups, and PBIEM can be employed as a macromolecular atom radical transfer polymerization (ATRP) initiator...
March 19, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29584656/magnetic-graphene-oxide-for-dual-targeted-delivery-of-doxorubicin-and-photothermal-therapy
#7
Yu-Jen Lu, Pin-Yi Lin, Pei-Han Huang, Chang-Yi Kuo, K T Shalumon, Mao-Yu Chen, Jyh-Ping Chen
To develop a pH-sensitive dual targeting magnetic nanocarrier for chemo-phototherapy in cancer treatment, we prepared magnetic graphene oxide (MGO) by depositing Fe₃O₄ magnetic nanoparticles on graphene oxide (GO) through chemical co-precipitation. MGO was modified with polyethylene glycol (PEG) and cetuximab (CET, an epidermal growth factor receptor (EGFR) monoclonal antibody) to obtain MGO-PEG-CET. Since EGFR was highly expressed on the tumor cell surface, MGO-PEG-CET was used for dual targeted delivery an anticancer drug doxorubicin (DOX)...
March 27, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29584410/a-universal-approach-to-render-nanomedicine-with-biological-identity-derived-from-cell-membranes
#8
Jingyi Zhu, Mingkang Zhang, Diwei Zheng, Sheng Hong, Jun Feng, Xian-Zheng Zhang
Biomimetic nanoengineering built through integrating the specific cell membrane with artificially synthetic nanomedicines represents one of the most promising directions for the actualization of personalized therapy. To address the technical hurdle against the development of this biomimetic technology, the present report describes the in-depth exploration and optimization over each critical preparation step, including establishment of nanoparticle-stabilized dispersion system, cargo loading, membrane coating and product isolation...
March 27, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29576709/antioxidant-and-hemolysis-protective-effects-of-polyphenol-rich-extract-from-mulberry-fruits
#9
Palanigounder Ganeshan Ajay Krishna, Thasma Raman Sivakumar, Chao Jin, Shao-Hu Li, Yu-Jie Weng, Juan Yin, Jun-Qiang Jia, Chu-Yan Wang, Zhong-Zheng Gui
Background: Mulberry fruits are a superior source of polyphenol, especially anthocyanins that contribute potentially to the beneficial effects which include reducing the risk of cardiovascular diseases and cancers with antioxidant, anti-inflammatory, and chemoprotective properties. Objectives: In this study, purification of the polyphenol-rich extract from mulberry fruit (MPE) was purified and assessed the activities of antioxidant and hemolysis protective in vivo and in vitro ...
January 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29576450/common-piezo1-allele-in-african-populations-causes-rbc-dehydration-and-attenuates-plasmodium-infection
#10
Shang Ma, Stuart Cahalan, Gregory LaMonte, Nathan D Grubaugh, Weizheng Zeng, Swetha E Murthy, Emma Paytas, Ramya Gamini, Viktor Lukacs, Tess Whitwam, Meaghan Loud, Rakhee Lohia, Laurence Berry, Shahid M Khan, Chris J Janse, Michael Bandell, Christian Schmedt, Kai Wengelnik, Andrew I Su, Eric Honore, Elizabeth A Winzeler, Kristian G Andersen, Ardem Patapoutian
Hereditary xerocytosis is thought to be a rare genetic condition characterized by red blood cell (RBC) dehydration with mild hemolysis. RBC dehydration is linked to reduced Plasmodium infection in vitro; however, the role of RBC dehydration in protection against malaria in vivo is unknown. Most cases of hereditary xerocytosis are associated with gain-of-function mutations in PIEZO1, a mechanically activated ion channel. We engineered a mouse model of hereditary xerocytosis and show that Plasmodium infection fails to cause experimental cerebral malaria in these mice due to the action of Piezo1 in RBCs and in T cells...
March 8, 2018: Cell
https://www.readbyqxmd.com/read/29556364/novel-self-assembled-organic-nanoprobe-for-molecular-imaging-and-treatment-of-gram-positive-bacterial-infection
#11
Tang Gao, Hongliang Zeng, Huan Xu, Feng Gao, Wei Li, Shengwang Zhang, Yi Liu, Guifang Luo, Mingdan Li, Dejian Jiang, Zhigao Chen, Yong Wu, Wei Wang, Wenbin Zeng
Background: Increasing bacterial infections as well as a rise in bacterial resistance call for the development of novel and safe antimicrobial agents without inducing bacterial resistance. Nanoparticles (NPs) present some advantages in treating bacterial infections and provide an alternative strategy to discover new antibiotics. Here, we report the development of novel self-assembled fluorescent organic nanoparticles ( FONs ) with excellent antibacterial efficacy and good biocompatibility. Methods: Self-assembly of 1-(12-(pyridin-1-ium-1-yl)dodecyl)-4-(1,4,5-triphenyl-1H-imidazol-2-yl)pyridin-1-ium (TPIP) in aqueous solution was investigated using dynamic light scattering (DLS) and transmission electron microscopy (TEM)...
2018: Theranostics
https://www.readbyqxmd.com/read/29550568/%C3%AE%C2%B5-poly-l-lysine-plasmid-dna-nanoplexes-for-efficient-gene-delivery-in-vivo
#12
Haimanti Mandal, Sameer S Katiyar, Rajan Swami, Varun Kushwah, Parmeshwar B Katare, Anand Kumar Meka, Sanjay K Banerjee, Amirali Popat, Sanyog Jain
The present work addresses the development and characterization of ε-Poly-l-Lysine/pDNA polyplexes and evaluation for their improved transfection efficacy and safety as compared to polyplexes prepared using Poly-l-Lysine and SuperFect®. Self-assembling polyplexes were prepared by varying the N/P ratio to obtain the optimum size, a net positive zeta potential and gel retardation. The stability in presence of DNase I and serum was assured using gel retardation assay. Their appreciable uptake in MCF-7 and 3...
March 14, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29530820/in-vitro-and-in-vivo-studies-on-zinc-hydroxyapatite-composites-as-novel-biodegradable-metal-matrix-composite-for-orthopedic-applications
#13
Hongtao Yang, Xinhua Qu, Wenjiao Lin, Cong Wang, Donghui Zhu, Kerong Dai, Yufeng Zheng
Recent studies indicate that there is a great demand to optimize pure Zn with tunable degradation rates and more desirable biocompatibility as orthopedic implants. Metal matrix composite (MMC) can be a promising approach for this purpose. In this study, MMC with pure Zn as a matrix and hydroxyapatite (HA) as reinforcements were prepared by spark plasma sintering (SPS). Feasibility of novel Zn-HA composites to be used as orthopedic implant applications was systematically evaluated. After sintering, HA distributed in the Zn particle boundaries uniformly...
March 9, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29522519/role-of-heme-in-lung-bacterial-infection-after-trauma-hemorrhage-and-stored-red-blood-cell-transfusion-a-preclinical-experimental-study
#14
Brant M Wagener, Parker J Hu, Joo-Yeun Oh, Cilina A Evans, Jillian R Richter, Jaideep Honavar, Angela P Brandon, Judy Creighton, Shannon W Stephens, Charity Morgan, Randal O Dull, Marisa B Marques, Jeffrey D Kerby, Jean-Francois Pittet, Rakesh P Patel
BACKGROUND: Trauma is the leading cause of death and disability in patients aged 1-46 y. Severely injured patients experience considerable blood loss and hemorrhagic shock requiring treatment with massive transfusion of red blood cells (RBCs). Preclinical and retrospective human studies in trauma patients have suggested that poorer therapeutic efficacy, increased severity of organ injury, and increased bacterial infection are associated with transfusion of large volumes of stored RBCs, although the mechanisms are not fully understood...
March 2018: PLoS Medicine
https://www.readbyqxmd.com/read/29504282/the-effect-of-xanthine-oxidase-and-hypoxanthine-on-the-permeability-of-red-cells-from-patients-with-sickle-cell-anemia
#15
Halima W M Al Balushi, David C Rees, John N Brewin, Anke Hannemann, John S Gibson
Red cells from patients with sickle cell anemia (SCA) are under greater oxidative challenge than those from normal individuals. We postulated that oxidants generated by xanthine oxidase (XO) and hypoxanthine (HO) contribute to the pathogenesis of SCA through altering solute permeability. Sickling, activities of the main red cell dehydration pathways (Psickle , Gardos channel, and KCl cotransporter [KCC]), and cell volume were measured at 100, 30, and 0 mmHg O2 , together with deoxygenation-induced nonelectrolyte hemolysis...
March 2018: Physiological Reports
https://www.readbyqxmd.com/read/29498054/evaluation-of-renal-oxygen-homeostasis-in-a-preclinical-animal-model-to-elucidate-difference-in-blood-quality-after-transfusion
#16
Jin Hyen Baek, Ayla Yalamanoglu, So-Eun Moon, Yamei Gao, Paul W Buehler
BACKGROUND: Red blood cell (RBC) oxygen (O2 ) delivery may be impacted at the tissue, cellular, and molecular levels after storage duration, preservation strategies, and pathogen reduction. Collectively, the preclinical measurement of arterial and venous PO2 , systemic blood flow, tissue hypoxia-inducible factors (HIFs), pimonidazole adduction, and erythropoietin (EPO) regulation can serve to elucidate differential RBC quality after storage and processing. STUDY DESIGN AND METHODS: Donor guinea pig blood was collected, leukoreduced, and stored at 4°C in AS-3 for 1 (fresh) or 14 (stored) days...
March 1, 2018: Transfusion
https://www.readbyqxmd.com/read/29496437/natural-polymer-functionalized-graphene-oxide-for-co-delivery-of-anticancer-drugs-in-vitro-and-in-vivo
#17
Ananya Deb, Nirmala Grace Andrews, Vimala Raghavan
The present study focuses on the development of a chitosan functionalized nanobiocomposite for the co-delivery of two anti-cancer drugs camptothecin (CPT) and 3,3'‑Diindolylmethane (DIM). The difference in the mechanism of action of the two drugs makes them a promising candidate to produce a synergistic effect against breast cancer. Herein a nanobiocomposite was developed by functionalizing a natural polymer chitosan to graphene oxide nanoparticles and decorated with folic acid. The nanobiocomposite thus synthesized was loaded with camptothecin and 3,3'‑Diindolylmethane and characterized by X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy (UV) and atomic force microscopy (AFM)...
February 26, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29463368/preparation-and-biocompatibility-evaluation-of-peg-pll-rgd-peg-dspe-phospholipid-cap-nanoparticles
#18
Jingwen Huang, Xiangyu Zhang, Zhihao Wu, Yufeng Wu, Xiaojie Wu, Yunguang Wang, Hao Jiang, Jing Ma, Zelai He
(Arginine-Glycine-Aspartic)-methoxy polyethylene glycol-(1,2-distearoyl-sn-glycero-3-phosphoethanolaMine-N) (abbreviation: RGD-PEG2000-DSPE or RGD-PD) was successfully synthesized and verified by 1H-NMR and MALDI-TOF MS. Polyethylene glycol-poly-L-lysine/RGD-PD/phospholipid/calcium phosphate nanoparticles (PEG-PLL/RGD-PD/PL/CaP NPs or MNPs) were prepared using a novel, simple method conducted at room temperature. Transmission electron microscopy (TEM) analysis showed that the MNPs were spheres of uniform size, with a diameter of ∼30 nm, and smooth surface...
January 1, 2018: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/29454237/liposomes-of-dimeric-artesunate-phospholipid-a-combination-of-dimerization-and-self-assembly-to-combat-malaria
#19
Muhammad Ismail, Longbing Ling, Yawei Du, Chen Yao, Xinsong Li
Artemisinin and its derivatives are highly effective drugs in the treatment of P. falciparum malaria. However, their clinical applications face challenges because of short half-life, poor bioavailability and growing drug resistance. In this article, novel dimeric artesunate phospholipid (Di-ART-GPC) based liposomes were developed by combination of dimerization and self-assembly to address these shortcomings. Firstly, Di-ART-GPC conjugate was synthesized by a facile esterification of artesunate (ART) and glycerophosphorylcholine (GPC) and confirmed by MS,1 H NMR and13 C NMR...
May 2018: Biomaterials
https://www.readbyqxmd.com/read/29454058/carboxymethyl-konjac-glucomannan-crosslinked-chitosan-sponges-for-wound-dressing
#20
Yu Xie, Zeng-Xing Yi, Jian-Xun Wang, Tong-Gang Hou, Qiong Jiang
In this paper, carboxymethyl konjac glucomannan (CMKGM) was obtained by carboxyl modification of konjac glucomannan. Then CMKGM and chitosan (CS) were crosslinked and freeze-dried to prepare CMKGM/CS composite sponges with different proportions. The structure and micromorphology of CMKGM/CS sponges were investigated by FTIR spectroscopy and SEM. The SEM results showed that the pore structure of the composite sponge gradually increased with the increase of CMKGM content. To assess the applicability of CMKGM/CS composite sponges as wound dressing, the swelling behavior, water vapor transmission rate (WVTR), biocompatibility (cytotoxicity and hemolysis) were analyzed...
February 14, 2018: International Journal of Biological Macromolecules
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