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in vivo hemolysis

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https://www.readbyqxmd.com/read/29020107/diacetylenic-lipids-in-the-design-of-stable-lipopolymers-able-to-complex-and-protect-plasmid-dna
#1
C Facundo Temprana, M Jimena Prieto, Daniela E Igartúa, A Lis Femia, M Silvia Amor, Silvia Del Valle Alonso
Different viral and non-viral vectors have been designed to allow the delivery of nucleic acids in gene therapy. In general, non-viral vectors have been associated with increased safety for in vivo use; however, issues regarding their efficacy, toxicity and stability continue to drive further research. Thus, the aim of this study was to evaluate the potential use of the polymerizable diacetylenic lipid 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DC8,9PC) as a strategy to formulate stable cationic lipopolymers in the delivery and protection of plasmid DNA...
2017: PloS One
https://www.readbyqxmd.com/read/28990203/drug-induced-immune-hemolytic-anemia-associated-with-anti-vancomycin-complicated-by-a-paraben-antibody
#2
Thomas J Gniadek, Patricia A Arndt, Regina M Leger, Daniel Zydowicz, Edward Y Cheng, Nicole D Zantek
BACKGROUND: Drug-induced immune hemolytic anemia (DIIHA) is rare, but potentially life-threatening. A high index of clinical suspicion is required for diagnosis, since the number of medications known to induce DIIHA continues to expand. Additionally, in vitro antibody reactivity against reagent additives has been reported, which may complicate test interpretation. CASE REPORT: A 61-year-old group A, D+ woman with a history of negative antibody detection tests developed hemolytic anemia on Postoperative Day 7 after repeat incision and drainage of a chronically infected right knee prosthesis...
October 8, 2017: Transfusion
https://www.readbyqxmd.com/read/28986321/an-optimized-two-vial-formulation-lipid-nanoemulsion-of-paclitaxel-for-targeted-delivery-to-tumor
#3
Lina Chen, Bingchen Chen, Li Deng, Baoan Gao, Yuansheng Zhang, Chan Wu, Nong Yu, Qinqin Zhou, Jianzhong Yao, Jianming Chen
The discovery of new intravenous drug delivery carrier for water-insoluble drug is a challenging task. In this paper, novel two-vial formulation of paclitaxel (PTX)-loaded lipid nanoemulsions (TPLEs) with particle sizes of 110nm (TPLE-1), 220nm (TPLE-2) and 380nm (TPLE-3), which were formed by mixing a PEG400 solution of PTX and 10% (w/w) blank lipid emulsions (BLEs) with different particle size prior to use, were developed and comparatively evaluated for their pharmaceutics, pharmacokinetics, biodistribution, in vitro and in vivo anticancer efficiency...
October 3, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28976179/trio-act-of-boronolectin-with-antibiotic-metal-complexed-macromolecules-toward-broad-spectrum-antimicrobial-efficacy
#4
Peng Yang, Marpe Bam, Parasmani Pageni, Tianyu Zhu, Yung Pin Chen, Mitzi Nagarkatti, Alan W Decho, Chuanbing Tang
Bacterial infections, particularly by Gram-negative pathogens, have become a serious threat to global healthcare due to the diminishing effectiveness of existing antibiotics. We report a nontraditional therapy to combine three components in one macromolecular system, in which boronic acid adheres to peptidoglycan or lipopolysaccharide via boron-polyol based boronolectin chemistry, cationic metal polymer frameworks interact with negatively charged cell membranes, and β-lactam antibiotics are reinstated with enhanced vitality to attack bacteria via evading the detrimental enzyme-catalyzed hydrolysis...
October 6, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28959860/-relationship-between-structure-and-function-of-cathelicidins-and-their-molecular-design-a-review
#5
Chen Wang, Lan Feng, Haining Yu, Yipeng Wang
Cathelicidins play critical roles in mammalian innate immune defense against invasive bacterial infection. In addition to their broad-spectrum bactericidal effect, cathelicidins are interesting peptide-based drug templates because they have multiple functions including anti-inflammatory, wound healing, and angiogenesis promotion. This article summarizes the aim and method of cathelicidin molecular designs. Residue mutation, fragment assembly, chemical modification, and construction of conjugates and dimers are usually used to increase the biological activities...
January 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28934662/a-pc-pu-nanoparticle-pu-decellularized-scaffold-composite-vascular-patch-synergistically-optimized-overall-performance-promotes-endothelialization
#6
Jun Zhang, Cheng Liu, Fuling Feng, Dawei Wang, Shuaishuai Lu, Guo Wei, Hong Mo, Tong Qiao
Composite vascular patches have gained increasingly attention due to the limited availability of autologous patches (vascular graft materials made from the blood vessels of the same recipient), the lack of growth capability of nonautologous patches (vascular graft materials made from the blood vessels of a different donor) and the disadvantages of synthetic patches. In this study, we report a highly biocompatible phosphatidylcholine-polyurethane nanoparticle/polyurethane/decellularized scaffold composite vascular patch (PCVP)...
September 6, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28932220/chalcone-attenuates-staphylococcus-aureus-virulence-by-targeting-sortase-a-and-alpha-hemolysin
#7
Bing Zhang, Zihao Teng, Xianhe Li, Gejin Lu, Xuming Deng, Xiaodi Niu, Jianfeng Wang
Staphylococcus aureus (S.aureus) resistance, considered a dilemma for the clinical treatment of this bacterial infection, is becoming increasingly intractable. Novel anti-virulence strategies will undoubtedly provide a path forward in combating these resistant bacterial infections. Sortase A (SrtA), an enzyme responsible for anchoring virulence-related surface proteins, and alpha-hemolysin (Hla), a pore-forming cytotoxin, have aroused great scientific interest, as they have been regarded as targets for promising agents against S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28920493/construction-and-evaluation-in-vitro-and-in-vivo-of-tedizolid-phosphate-loaded-cationic-liposomes
#8
Zhenlei Yang, Liu Tian, Jingjing Liu, Guihua Huang
Firstly, The SA-TDZA-Lips were prepared by reverse-phase evaporation method. Then, the drug release behavior was evaluated by dynamic membrane dialysis in vitro and the preliminary safety was evaluated by hemolysis method. Finally, with Tedizolid phosphate injection (TDZA-Inj) and tedizolid phosphate loaded liposomes (TDZA-Lips) as the control groups, the pharmacokinetic characteristic and tissues distribution of SA-TDZA-Lips were evaluated after intravenous injection. As a result, the stearylamine modified tedizolid phosphate liposomal delivery system was constructed successfully and the particle size was (194...
September 18, 2017: Journal of Liposome Research
https://www.readbyqxmd.com/read/28911659/the-detoxifying-effects-of-structural-elements-of%C3%A2-persimmon-tannin-on-chinese-cobra-phospholipase-a2-correlated-with-their-structural-disturbing-effects-well
#9
Ying Zhang, Chun-Mei Li
The effects of persimmon tannin (PT) characteristic structural elements on Naja atra phospholipase A2 (PLA2)-induced lethality, myotoxicity, and hemolysis in mice models were determined. In addition, methods including surface plasmon resonance, dynamic light scattering, and Fourier transform infrared spectroscopy were explored to uncover the possible detoxifying mechanisms of PT on snake venom PLA2. Our results revealed that PT characteristic elements (EGCG, ECG, A-type EGCG dimer, and A-type ECG dimer) could neutralize the lethality, myotoxicity, and hemolysis of PLA2...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28903180/diamine-derivative-anti-trichomonas-vaginalis-and-anti-tritrichomonas-foetus-activities-by-effect-on-polyamine-metabolism
#10
Graziela Vargas Rigo, Márcia Rodrigues Trein, Danielle da Silva Trentin, Alexandre José Macedo, Bruno Assis de Oliveira, Angelina Maria de Almeida, Raquel Brandt Giordani, Mauro Vieira de Almeida, Tiana Tasca
Human and bovine trichomoniasis are sexually transmitted diseases (STD) caused by Trichomonas vaginalis and Tritrichomonas foetus, respectively. Human trichomoniasis is the most common non-viral STD in the world and bovine trichomoniasis causes significant economic losses to breeders. Considering the significant impact of the infections caused by these protozoa and the treatment failures, the search for new therapeutic alternatives becomes crucial. In this study the effect of diamines and amino alcohols in the in vitro viability of trichomonads was evaluated...
September 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28894233/a-ph-dependent-antibacterial-peptide-release-nano-system-blocks-tumor-growth-in-vivo-without-toxicity
#11
Jing Cao, Yan Zhang, Yanke Shan, Jingui Wang, Fei Liu, Hongrui Liu, Gang Xing, Jing Lei, Jiyong Zhou
In this study, we designed a nano-system where a novel antibacterial peptide RGD-hylin a1 with reduced hemolysis than the commonly studied melittin was loaded onto mesoporous silica (HMS). We found out that the designed nano-system, RGD-hylin a1-HMS, released RGD-hylin a1 in a pH-dependent manner. It caused apoptosis of cancer cells at low dosage of the antibacterial peptide at pH = 5.5, but was safe to the cells at pH = 7. The hemolytic activity of RGD-hylin a1 itself was reduced by 50~100% by the nano-system depending on the dosage...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28890388/dhea-prevents-ribavirin-induced-anemia-via-inhibition-of-glucose-6-phosphate-dehydrogenase
#12
Lynda Handala, Barbara Domange, Hakim Ouled-Haddou, Loïc Garçon, Eric Nguyen-Khac, Francois Helle, Sandra Bodeau, Gilles Duverlie, Etienne Brochot
Ribavirin has been widely used for antiviral therapy. Unfortunately, ribavirin-induced anemia is often a cause of limiting or interrupting treatment. Our team has observed that dehydroepiandrosterone (DHEA) has a protective effect against in vitro and in vivo ribavirin-induced hemolysis. The aim of this study was to better understand this effect as well as the underlying mechanism(s). DHEA was able to reduce in vitro intraerythrocytic ATP depletion induced by ribavirin. Only 1% of ATP remained after incubation with ribavirin (2 mM) at 37 °C for 24 h vs...
September 8, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28887121/discovery-of-human-scfvs-that-cross-neutralize-the-toxic-effects-of-b-jararacussu-and-c-d-terrificus-venoms
#13
Luciano C Silva, Manuela B Pucca, Gabriela Pessenda, Lucas B Campos, Edson Z Martinez, Felipe A Cerni, José E Barbosa
Accidents involving venomous snakes are a public health problem worldwide, causing a large number of deaths per year. In Brazil, the majority of accidents are caused by the Bothrops and Crotalus genera, which are responsible for approximately 80% of severe envenoming cases. The cross-neutralization of snake venoms by antibodies is an important issue for development of more effective treatments. Our group has previously reported the construction of human monoclonal antibody fragments towards Bothrops jararacussu and Crotalus durissus terrificus' venoms...
September 5, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28866216/ultrasonically-assisted-preparation-of-poly-acrylic-acid-calcium-phosphate-hybrid-nanogels-as-ph-responsive-drug-carriers
#14
Fang Li, Qingguo Xing, Yingchao Han, Yue Li, Wei Wang, Thalagalage Shalika Harshani Perera, Honglian Dai
Biocompatible, biodegradable and stimuli-responsive nanomaterials can be used as drug carriers and to achieve controlled drug delivery, which is crucial for treating tumors and lowering drug side effects. Calcium phosphate (CaP) nanoparticles and poly(acrylic acid) (PAA) hydrogels can be used as biocompatible and pH-responsive drug carriers. In this study, based on the ultrasound effect, PAA/CaP hybrid nanogels (approximately 100nm, PDI<0.2) are obtained via the cross-linking of CaP nanoparticles and PAA molecules between the Ca(2+) ions and -COOH groups...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28851583/chemical-transport-knockout-for-oxidized-vitamin-c-dehydroascorbic-acid-reveals-its-functions-in-vivo
#15
Hongbin Tu, Yu Wang, Hongyan Li, Lauren R Brinster, Mark Levine
Despite its transport by glucose transporters (GLUTs) in vitro, it is unknown whether dehydroascorbic acid (oxidized vitamin C, DHA) has any in vivo function. To investigate, we created a chemical transport knockout model using the vitamin C analog 6-bromo-ascorbate. This analog is transported on sodium-dependent vitamin C transporters but its oxidized form, 6-bromo-dehydroascorbic acid, is not transported by GLUTs. Mice (gulo(-/-)) unable to synthesize ascorbate (vitamin C) were raised on 6-bromo-ascorbate...
September 2017: EBioMedicine
https://www.readbyqxmd.com/read/28797532/assessment-of-in-vitro-antifungal-efficacy-and-in-vivo-toxicity-of-amphotericin-b-loaded-plga-and-plga-peg-blend-nanoparticles
#16
T C Moraes Moreira Carraro, C Altmeyer, N Maissar Khalil, R Mara Mainardes
Amphotericin B (AmB) is widely applied in treatment of systemic fungal infections. However, the emergence of severe adverse effects, such as nephrotoxicity, hepatotoxicity and hemolytic anemia, can limit its clinical use. Poly(lactide-co-glycolide) (PLGA) or poly(lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG) blend nanoparticles containing AmB were developed with the aim to decrease AmB toxicity and propose the oral route for AmB delivery. Nanoparticles were characterized by particle size, polydispersity index, Fourier transform infrared spectroscopy, differential scanning calorimetry and X-ray diffraction analyses...
August 7, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28789838/comparison-of-biochemical-and-cytotoxic-activities-of-extracts-obtained-from-dorsal-spines-and-caudal-fin-of-adult-and-juvenile-non-native-caribbean-lionfish-pterois-volitans-miles
#17
Aránzazu Sáenz, Natalia Ortiz, Bruno Lomonte, Alexandra Rucavado, Cecilia Díaz
Pterois volitans/miles lionfish (adult and juvenile) dorsal spines and caudal fin extracts were compared in their general composition, enzymatic activities and hemolytic and cytotoxic effects on bovine aortic endothelial cells and murine myoblasts, to distinguish between the activities present in the venom and epidermal mucus. Intradermal and intramuscular injections were also administered in mice to determine in vivo effects. This work shows that crude venom of Caribbean species of lionfish, present in dorsal spines, induces several in vitro effects including hemolysis, weak cytotoxicity, proteolytic and hyaluronidase activities, whereas in vivo, it is not hemorrhagic nor myotoxic, but causes edema, plasma extravasation and a thrombotic-associated lesion on the skin...
August 5, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28780414/chitosan-coated-pluronicf127-micelles-for-effective-delivery-of-amphotericin-b-in-experimental-visceral-leishmaniasis
#18
Pankaj K Singh, Vivek K Pawar, Anil K Jaiswal, Yuvraj Singh, Cheruvu Hanumanth Srikanth, Mohini Chaurasia, Himangsu K Bora, Kavit Raval, Jaya Gopal Meher, Jiaur R Gayen, Anuradha Dube, Manish K Chourasia
The goal of study was to develop micellar formulation of Amphotericin B (AmB) to improve its antileishmanial efficacy. AmB loaded pluronic F127 (PF 127) micelles were developed and coated with chitosan (Cs-PF-AmB-M) to accord immunoadjuvant and macrophage targeting properties. Hemolysis and cytotoxicity studies demonstrated that Cs-PF-AmB-M was 7.93 fold (at 20μg/ml AmB concentration) and 9.35 fold less hemolytic and cytotoxic, respectively in comparison to AmB suspension. Flow cytometry studies indicated that Cs-PF-FITC-M was 21...
August 2, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28769560/non-immunogenic-dextran-coated-superparamagnetic-iron-oxide-nanoparticles-a-biocompatible-size-tunable-contrast-agent-for-magnetic-resonance-imaging
#19
Harald Unterweger, Christina Janko, Marc Schwarz, László Dézsi, Rudolf Urbanics, Jasmin Matuszak, Erik Őrfi, Tamás Fülöp, Tobias Bäuerle, János Szebeni, Clément Journé, Aldo R Boccaccini, Christoph Alexiou, Stefan Lyer, Iwona Cicha
Iron oxide-based contrast agents have been in clinical use for magnetic resonance imaging (MRI) of lymph nodes, liver, intestines, and the cardiovascular system. Superparamagnetic iron oxide nanoparticles (SPIONs) have high potential as a contrast agent for MRI, but no intravenous iron oxide-containing agents are currently approved for clinical imaging. The aim of our work was to analyze the hemocompatibility and immuno-safety of a new type of dextran-coated SPIONs (SPIONdex) and to characterize these nanoparticles with ultra-high-field MRI...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28766856/alkyl-chitosan-film-high-strength-functional-biomaterials
#20
Li Lu, Cao Xing, Shen Xin, Yu Shitao, Su Feng, Liu Shiwei, Liu Fusheng, Xie Congxia
Biofilm with strong tensile strength is a topic item in the area of tissue engineering, medicine engineering, and so forth. Here we introduced an alkyl chitosan film with strong tensile strength and its possibility for an absorbable anticoagulation material in vivo was tested in the series of blood test, such as dynamic coagulation time, plasma recalcification time and hemolysis. Alkyl chitosan film was a better biomaterial than traditional chitosan film in the anticoagulation, tissue compatibility and cell compatibility...
August 2, 2017: Journal of Biomedical Materials Research. Part A
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