keyword
MENU ▼
Read by QxMD icon Read
search

Cd45

keyword
https://www.readbyqxmd.com/read/27914035/mobilization-of-peripheral-blood-stem-cells-and-changes-in-the-concentration-of-plasma-factors-influencing-their-movement-in-patients-with-panic-disorder
#1
Marcin Jabłoński, Jolanta Kucharska Mazur, Maciej Tarnowski, Barbara Dołęgowska, Daniel Pędziwiatr, Ewa Kubiś, Marta Budkowska, Daria Sałata, Justyna Pełka Wysiecka, Arkadiusz Kazimierczak, Artur Reginia, Mariusz Z Ratajczak, Jerzy Samochowiec
In this paper we examined whether stem cells and factors responsible for their movement may serve as new biological markers of anxiety disorders. The study was carried out on a group of 30 patients diagnosed with panic disorder (examined before and after treatment), compared to 30 healthy individuals forming the control group. We examined the number of circulating HSCs (hematopoetic stem cells) (Lin-/CD45 +/CD34 +) and HSCs (Lin-/CD45 +/AC133 +), the number of circulating VSELs (very small embryonic-like stem cells) (Lin-/CD45-/CD34 +) and VSELs (Lin-/CD45-/AC133 +), as well as the concentration of complement components: C3a, C5a and C5b-9, SDF-1 (stromal derived factor) and S1P (sphingosine-1-phosphate)...
December 2, 2016: Stem Cell Reviews
https://www.readbyqxmd.com/read/27913649/identification-and-function-of-fibrocytes-in-skeletal-muscle-injury-repair-and-muscular-dystrophy
#2
Xingyu Wang, Wanming Zhao, Richard M Ransohoff, Lan Zhou
We identified and characterized the function of CD45(+)/collagen I(+) fibrocytes in acutely injured skeletal muscle of wild-type (WT) and Ccr2(-/-) mice, and in quadriceps and diaphragm muscles of mdx(5cv) mice, a mouse model for Duchenne muscular dystrophy. Fibrocytes were not detected in peripheral blood of WT mice after acute muscle injury or mdx(5cv) mice. Fibrocytes were detected in acutely injured muscles and in mdx(5cv) quadriceps and diaphragm muscles. These cells expressed F4/80 and CCR2, and they were mostly Ly6C(lo) They expressed a low level of collagens but a high level of profibrotic growth factors as compared with i...
December 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27907211/characterization-and-immunomodulatory-effects-of-canine-adipose-tissue-and-bone-marrow-derived-mesenchymal-stromal-cells
#3
Keith A Russell, Natalie H C Chow, David Dukoff, Thomas W G Gibson, Jonathan LaMarre, Dean H Betts, Thomas G Koch
BACKGROUND: Mesenchymal stromal cells (MSC) hold promise for both cell replacement and immune modulation strategies owing to their progenitor and non-progenitor functions, respectively. Characterization of MSC from different sources is an important and necessary step before clinical use of these cells is widely adopted. Little is known about the biology and function of canine MSC compared to their mouse or human counterparts. This knowledge-gap impedes development of canine evidence-based MSC technologies...
2016: PloS One
https://www.readbyqxmd.com/read/27906115/established-cell-surface-markers-efficiently-isolate-highly-overlapping-populations-of-skeletal-muscle-satellite-cells-by-fluorescence-activated-cell-sorting
#4
Claire C Maesner, Albert E Almada, Amy J Wagers
BACKGROUND: Fluorescent-activated cell sorting (FACS) has enabled the direct isolation of highly enriched skeletal muscle stem cell, or satellite cell, populations from postnatal tissue. Several distinct surface marker panels containing different positively selecting surface antigens have been used to distinguish muscle satellite cells from other non-myogenic cell types. Because functional and transcriptional heterogeneity is known to exist within the satellite cell population, a direct comparison of results obtained in different laboratories has been complicated by a lack of clarity as to whether commonly utilized surface marker combinations select for distinct or overlapping subsets of the satellite cell pool...
November 8, 2016: Skeletal Muscle
https://www.readbyqxmd.com/read/27903712/the-myeloma-stem-cell-concept-revisited-from-phenomenology-to-operational-terms
#5
REVIEW
Hans Erik Johnsen, Martin Bøgsted, Alexander Schmitz, Julie Støve Bødker, Tarec Christoffer El-Galaly, Preben Johansen, Peter Valent, Niklas Zojer, Els Van Valckenborgh, Karin Vanderkerken, Mark van Duin, Pieter Sonneveld, Martin Perez-Andres, Alberto Orfao, Karen Dybkær
The concept of the myeloma stem cell may have important therapeutic implications, yet its demonstration has been hampered by a lack of consistency in terms and definitions. Here, we summarize the current documentation and propose single-cell in vitro studies for future translational studies. By the classical approach, a CD19(-)/CD45(low/-)/CD38(high)/CD138(+) malignant plasma cell, but not the CD19(+)/CD38(low/-) memory B cell compartment, is enriched for tumorigenic cells that initiate myeloma in xenografted immunodeficient mice, supporting that myeloma stem cells are present in the malignant PC compartment...
December 2016: Haematologica
https://www.readbyqxmd.com/read/27899395/functional-definition-of-progenitors-versus-mature-endothelial-cells-reveals-key-soxf-dependent-differentiation-process
#6
Jatin Patel, Elke J Seppanen, Mathieu P Rodero, Ho Yi Wong, Prudence Donovan, Zoltan Neufeld, Nicholas M Fisk, Mathias François, Kiarash Khosrotehrani
BACKGROUND: -During adult life, blood vessel formation is thought to occur via angiogenic processes involving branching from existing vessels. An alternate proposal suggests that neo-vessels form from endothelial progenitors able to assemble the intimal layers. We here aimed to define vessel-resident endothelial progenitors in vivo in a variety of tissues in physiological and pathological situations such as normal aorta, lungs, as well as wound healing, tumors and placenta. METHODS: -Based on protein expression levels of common endothelial markers using flow cytometry, three sub-populations of endothelial cells could be identified among VE-Cadherin+ and CD45- cells...
November 29, 2016: Circulation
https://www.readbyqxmd.com/read/27895854/differential-expression-of-aristaless-like-homeobox-4-a-potential-marker-for-gastric-adenocarcinoma
#7
Fariba Ghasemvand, Navid Nezafat, Saeed Hesami Tackallou, Daruosh Momenzadeh, Saeid Rahmanzadeh
AIM: The objective of this experiment was to evaluate the ALX-4 mRNA expression level in different stages of human gastric adenocarcinoma compared to the gastric cancer stem cells (GCSC) and gastric cancer cell line, MKN-45. BACKGROUND: Gastric cancer is the second most common cancer in the world today, leading approximately to 3-10% of all cancer-related deaths. Identification of specific biomarkers could be a crucial approach to improve diagnosis and treatment of this cancer type...
2016: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/27893630/a-comprehensive-in-vitro-comparison-of-preparation-techniques-for-fat-grafting
#8
Libor Streit, Josef Jaros, Veronika Sedlakova, Miroslava Sedlackova, Lubos Drazan, Michal Svoboda, Jakub Pospisil, Tomas Vyska, Jiri Vesely, Ales Hampl
BACKGROUND: Lipomodelling is a technique that uses patient's own fat for tissue regeneration and augmentation. The extent of regenerative effect is reported to be determined by the numbers of adipose tissue-derived stem cells (ASCs) and the viability of cells in processed adipose tissue, which, together with other factors, influence the degree of graft retention. This study addresses whether differences exist in properties of fat graft obtained by three commonly used techniques. METHODS: Adipose tissue harvested from the hypo-gastric regions of 14 patients was processed by decantation, centrifugation, and membrane-based tissue filtration...
November 21, 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27889920/neuropeptide-y-receptor-1-is-expressed-by-b-and-t-lymphocytes-and-mast-cells-in-infantile-haemangiomas
#9
Elysia M S Tan, Max G Blackwell, Jonathan C Dunne, Reginald Marsh, Swee T Tan, Tinte Itinteang
AIM: We investigated the expression of neuropeptide Y (NPY), NPY receptor 1 (NPYR1) and NPY receptor 2 (NPYR2) in infantile haemangiomas (IHs). METHODS: Immunohistochemical (IHC) staining was performed on proliferating IHs from six patients aged 4-13 (mean 8.7) months and involuted IHs from six patients aged 5-59 (mean 18.7) years, for the expression of NPY, NPYR1 and NPYR2. Protein and messenger ribonucleic acid expression corresponding to these proteins was investigated by Western blotting and NanoString analysis, respectively...
November 27, 2016: Acta Paediatrica
https://www.readbyqxmd.com/read/27889762/evaluation-of-isolation-methods-for-circulating-tumor-cells-ctcs
#10
Galatea Kallergi, Eleni Politaki, Saad Alkahtani, Christos Stournaras, Vassilis Georgoulias
BACKGROUND: Detection of CTCs is a poor prognostic factor for many cancer types; however, their very low frequency represents an obstacle for their detection. The objective of the current study was to compare the performance of commonly used methods for CTCs isolation. METHODS: The evaluated methods using spiking experiments of MCF7, SKBR3 and MDA MB-231 breast cancer cell lines were (i) ficoll density gradient separation (DGS), (ii) red blood cell lysis (Erythrolysis) isolation, (iii) positive immunomagnetic selection (EpCAM Dynal beads), (iv) two different negative immunomagnetic separation systems (Dynal vs Miltenyi CD45 beads) as well as (v) the Cell Search platform and (vi) the ISET system...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27888664/ebv-associated-but-hhv8-unrelated-double-hit-effusion-based-lymphoma
#11
Bo-Jung Chen, David Yen-Ting Chen, Chun-Chi Kuo, Shih-Sung Chuang
Effusion-based lymphoma is a rare and unique type of large B-cell lymphoma presenting in effusion without a mass lesion. It shares many clinicopathological features with primary effusion lymphoma (PEL), but is distinct from PEL by the absence of HHV8 association. Double hit lymphoma (DHL) is an aggressive B-cell lymphoma, defined by concurrent rearrangement of MYC and BCL2 or BCL6. DHL often presents as lymphadenopathy or an extranodal mass, but rarely occurs in effusion. Here we report a 61-year-old male with alcoholic cirrhosis presenting as massive ascites and left pleural effusion...
November 26, 2016: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/27887984/impact-of-%C3%AE-hydroxy-%C3%AE-methylbutyrate-hmb-on-age-related-functional-deficits-in-mice
#12
Michael Munroe, Yair Pincu, Jennifer Merritt, Adam Cobert, Ryan Brander, Tor Jensen, Justin Rhodes, Marni D Boppart
β-Hydroxy β-methylbutyrate (HMB) is a metabolite of the essential amino acid leucine. Recent studies demonstrate a decline in plasma HMB concentrations in humans across the lifespan, and HMB supplementation may be able to preserve muscle mass and strength in older adults. However, the impact of HMB supplementation on hippocampal neurogenesis and cognition remains largely unexplored. The purpose of this study was to simultaneously evaluate the impact of HMB on muscle strength, neurogenesis and cognition in young and aged mice...
November 22, 2016: Experimental Gerontology
https://www.readbyqxmd.com/read/27887719/leucine-rich-repeat-containing-g-protein-coupled%C3%A2-receptor%C3%A2-5-positive%C3%A2-cells%C3%A2-in-the-endometrial-stem-cell-niche
#13
Irene Cervelló, Claudia Gil-Sanchis, Xavier Santamaría, Amparo Faus, Julia Vallvé-Juanico, Patricia Díaz-Gimeno, Oriana Genolet, Antonio Pellicer, Carlos Simón
OBJECTIVE: To study, isolate and characterize leucine-rich repeat-containing heterotrimeric guanine nucleotide-binding protein-coupled receptor 5 (LGR5)-positive cells from human endometrium to determine their functional relevance. DESIGN: Prospective experimental animal study. SETTING: University research laboratories. ANIMAL(S): Nonobese diabetic mice (NOD-SCID) (strain code 394; NOD.CB17-Prkdc(scid)/NcrCrl). INTERVENTION(S): Human LGR5(+) cells were labeled with superparamagnetic iron oxide nanoparticles (SPIOs) and injected under the kidney capsule in immunocompromised mice...
November 22, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27886006/semi-automated-extraction-and-characterization-of-stromal-vascular-fraction-using-a-new-medical-device
#14
Alexander Hanke, Lukas Prantl, Carina Wenzel, Michael Nerlich, Gero Brockhoff, Markus Loibl, Sebastian Gehmert
INTRODUCTION: The stem cell rich Stromal Vascular Fraction (SVF) can be harvested by processing lipo-aspirate or fat tissue with an enzymatic digestion followed by centrifugation. To date neither a standardised extraction method for SVF nor a generally admitted protocol for cell application in patients exists. A novel commercially available semi-automated device for the extraction of SVF promises sterility, consistent results and usability in the clinical routine. The aim of this work was to compare the quantity and quality of the SVF between the new system and an established manual laboratory method...
November 23, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27884655/zika-virus-infection-in-dexamethasone-immunosuppressed-mice-demonstrating-disseminated-infection-with-multi-organ-involvement-including-orchitis-effectively-treated-by-recombinant-type-i-interferons
#15
Jasper Fuk-Woo Chan, Anna Jinxia Zhang, Chris Chung-Sing Chan, Cyril Chik-Yan Yip, Winger Wing-Nga Mak, Houshun Zhu, Vincent Kwok-Man Poon, Kah-Meng Tee, Zheng Zhu, Jian-Piao Cai, Jessica Oi-Ling Tsang, Kenn Ka-Heng Chik, Feifei Yin, Kwok-Hung Chan, Kin-Hang Kok, Dong-Yan Jin, Rex Kwok-Him Au-Yeung, Kwok-Yung Yuen
BACKGROUND: Disseminated or fatal Zika virus (ZIKV) infections were reported in immunosuppressed patients. Existing interferon-signaling/receptor-deficient mouse models may not be suitable for evaluating treatment effects of recombinant interferons. METHODS: We developed a novel mouse model for ZIKV infection by immunosuppressing BALB/c mice with dexamethasone. RESULTS: Dexamethasone-immunosuppressed male mice (6-8weeks) developed disseminated infection as evidenced by the detection of ZIKV-NS1 protein expression and high viral loads in multiple organs...
November 12, 2016: EBioMedicine
https://www.readbyqxmd.com/read/27882430/identification-of-cardiovascular-risk-factors-associated-with-bone-marrow-cell-subsets-in-patients-with-stemi-a-biorepository-evaluation-from-the-cctrn-time-and-latetime-clinical-trials
#16
Ariadna Contreras, Aaron F Orozco, Micheline Resende, Robert C Schutt, Jay H Traverse, Timothy D Henry, Dejian Lai, John P Cooke, Roberto Bolli, Michelle L Cohen, Lem Moyé, Carl J Pepine, Phillip C Yang, Emerson C Perin, James T Willerson, Doris A Taylor
Autologous bone marrow mononuclear cell (BM-MNC) therapy for patients with ST-segment elevation myocardial infarction (STEMI) has produced inconsistent results, possibly due to BM-MNC product heterogeneity. Patient-specific cardiovascular risk factors (CRFs) may contribute to variations in BM-MNC composition. We sought to identify associations between BM-MNC subset frequencies and specific CRFs in STEMI patients. Bone marrow was collected from 191 STEMI patients enrolled in the CCTRN TIME and LateTIME trials...
January 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/27882356/intra-osseous-co-transplantation-of-cd34-selected-umbilical-cord-blood-and-mesenchymal-stromal-cells
#17
Leland Metheny, Saada Eid, Karen Lingas, Jane Reese, Howard Meyerson, Alexander Tong, Marcos de Lima, Alex Y Huang
Human mesenchymal stromal cells (MSC) have been shown to support the growth and differentiation of hematopoietic stem cells (HSC). We hypothesized that intra-osseous (IO) co-transplantation of MSC and umbilical cord blood (UCB) may be effective in improving early HSC engraftment, as IO transplantation has been demonstrated to enhance UCB engraftment in NOD SCID-gamma (NSG) mice. Following non-lethal irradiation (300rads), 6 groups of NSG mice were studied: 1) intravenous (IV) UCB CD34(+) cells, 2) IV UCB CD34(+) cells and MSC, 3) IO UCB CD34(+) cells, 4) IO UCB CD34(+) cells and IO MSC, 5) IO UCB CD34(+) cells and IV MSC, and 6) IV UCB CD34(+) and IO MSC...
2016: Hematol Med Oncol
https://www.readbyqxmd.com/read/27878497/role-of-tissue-specific-stem-and-progenitor-cells-in-the-regeneration-of-the-pancreas-and-testicular-tissue-in-diabetic-disorders
#18
E G Skurikhin, A V Pakhomova, N N Ermakova, O V Pershina, V A Krupin, E S Pan, A I Kudryashova, L A Ermolaeva, A M Dygai
Using the model of hypogonadism in C57Bl/6 male mice, we showed that injection of streptozotocin to newborn animals and high-fat diet induced serum IFN-γ and IL-17 elevation, glucose metabolism disturbances, insulin resistance, destructive changes of the Langerhans islets (deficit of PDX1(+)β cells), while the number of oligopotent β cell precursors (CD45(-)TER119(-)CD133(+)CD49f(low)) increased. Diabetes played the role of an inducer of testicular tissue inflammation (pan-hemopoietic cell infiltration, increase of IL-2, IL-17, and IL-23 content) and reproductive system disturbances in mice (decrease in free testosterone concentration, suppression of spermatogenesis, and infertility)...
November 23, 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27878106/multiparameter-evaluation-of-the-heterogeneity-of-circulating-tumor-cells-using-integrated-rna-in-situ-hybridization-and-immunocytochemical-analysis
#19
Yongqi Wu, Kyoung-Joo Jenny Park, Clayton Deighan, Peter Amaya, Brandon Miller, Quintin Pan, Maciej Zborowski, Maryam Lustberg, Jeffery Chalmers
Circulating tumor cells (CTCs) are routinely identified as cytokeratin (CK)-positive, epithelial cell adhesion molecule (EpCAM)-positive, and CD45-negative and are enriched based on EpCAM. However, there are a number of methodological challenges regarding both isolation and characterization of these rare CTCs including downregulation or absence of EpCAM in a variety of solid tumors leading to the omission of subpopulations of CTCs, difficulties in analyzing RNA and protein targets in CTCs due to the rarity of these cells, and low levels of targets and technological limitations of visualizing the targets of interest on each individual cell...
2016: Frontiers in Oncology
https://www.readbyqxmd.com/read/27875939/injectable-shape-memorizing-3d-hyaluronic-acid-cryogels-3d-ma-ha-for-skin-sculpting-and-soft-tissue-reconstruction
#20
Liying Cheng, Kai Ji, Ting-Yu Shih, Anthony Haddad, Giorgio Giatsidis, David J Mooney, Dennis P Orgill, Christoph S Nabzdyk
Introduction Hyaluronic acid based (HA) fillers are used for various cosmetic procedures. However, due to filler migration and degradation, reinjections of the fillers are often required. Methacrylated HA (MA-HA) can be made into injectable, shape-memorizing fillers (3D MA-HA) aimed to address this issue. In this study shape retention, firmness and biocompatibility of 3D MA-HA injected subcutaneously in mice were evaluated. Methods Fifteen mice, each receiving two subcutaneous injections in their back, were divided into 4 groups receiving HA, MA-HA, 3D MA-HA or saline, respectively...
November 22, 2016: Tissue Engineering. Part A
keyword
keyword
95966
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"