keyword
MENU ▼
Read by QxMD icon Read
search

Cd45

keyword
https://www.readbyqxmd.com/read/29351404/type-2-diabetes-impairs-the-ability-of-skeletal-muscle-pericytes-to-augment-postischemic-neovascularization-in-db-db-mice
#1
Katherine L Hayes, Louis M Messina, Lawrence M Schwartz, Jinglian Yan, Amy S Burnside, Sarah Witkowski
Peripheral artery disease is an atherosclerotic occlusive disease that causes limb ischemia and has few effective non-interventional treatments. Stem cell therapy is promising, but concomitant diabetes may limit its effectiveness. We evaluated the therapeutic potential of skeletal muscle pericytes to augment postischemic neovascularization in wild type and type 2 diabetic (T2DM) mice. Wild type C57BL/6J and leptin receptor spontaneous mutation db/db T2DM mice underwent unilateral femoral artery excision to induce limb ischemia...
January 10, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29351393/liver-tissue-metabolically-transformed-by-alcohol-induces-immune-recognition-of-liver-self-proteins-but-not-in-vivo-inflammation
#2
Michael J Duryee, Benjamin M Wiese, Jordan R Bowman, Jared D Vanlandingham, Lynell W Klassen, Geoffrey E Thiele, Carlos D Hunter, Daniel R Anderson, Ted R Mikuls, Geoffrey M Thiele
Precision cut liver slices (PCLSs) provide a novel model for studies in alcoholic liver disease (ALD). This is relevant, as in vivo ethanol exposure does not appear to generate significant liver damage in ethanol fed mice, except in the NIAAA binge model of ALD. Previous studies have shown that the two metabolites of ethanol consumption, malondialdhyde (MDA) and acetaldehyde (AA), combine to form (MAA), which has been correlated with the development and progression of ALD. In this study murine PCLSs were incubated with ethanol and examined for the production of MAA...
December 28, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/29350405/reactive-oxygen-species-induced-parthanatos-of-immunocytes-by-human-cytomegalovirus-associated-substance
#3
Jung Heon Kim, Jiyeon Kim, Jin Roh, Chan-Sik Park, Ju-Young Seoh, Eung-Soo Hwang
Previous studies examined various immune evasion strategies of human cytomegalovirus (HCMV) to understand HCMV pathogenesis. Although the mechanism that underlies immunocyte destruction near HCMV-infected lesions has yet to be established, we show that substances produced by HCMV-infected cells induce death in several types of immunocytes, but not in fibroblasts or astrocytomas. These substances contain HCMV proteins and were termed HCMV-associated insoluble substance (HCMVAIS). We characterized this mechanism of cell death induced by HCMVAIS to understand the death of immunocytes near HCMV-infected lesions...
January 19, 2018: Microbiology and Immunology
https://www.readbyqxmd.com/read/29345024/being-pluripotent-bone-marrow-vsels-rather-than-hscs-have-the-potential-to-regenerate-other-adult-organs
#4
REVIEW
Deepa Bhartiya
Recent study by Deanne's group provides evidence that transplanted GFP positive bone marrow hematopoietic stem cells (HSCs) expressing CD45 do not regenerate the endometrium of irradiated mice. The results do not surprise us since global efforts to regenerate various organs by transplanting bone marrow (BM) mononuclear cells (presumably enriched for HSCs) have failed. HSCs can only regenerate/reconstitute BM as they are 'committed progenitors' that are specified from more primitive, CD45 negative stem cells...
January 18, 2018: Stem Cells
https://www.readbyqxmd.com/read/29342188/comparative-analysis-of-gene-expression-identifies-distinct-molecular-signatures-of-bone-marrow-and-periosteal-skeletal-stem-progenitor-cells
#5
Lorenzo Deveza, Laura Ortinau, Kevin Lei, Dongsu Park
Periosteum and bone marrow (BM) both contain skeletal stem/progenitor cells (SSCs) that participate in fracture repair. However, the functional difference and selective regulatory mechanisms of SSCs in different locations are unknown due to the lack of specific markers. Here, we report a comprehensive gene expression analysis of bone marrow SSCs (BM-SSCs), periosteal SSCs (P-SSCs), and more differentiated osteoprogenitors by using reporter mice expressing Interferon-inducible Mx1 and NestinGFP, previously known SSC markers...
2018: PloS One
https://www.readbyqxmd.com/read/29341972/ocular-surface-and-tear-film-characteristics-in-a-sclerodermatous-chronic-graft-versus-host-disease-mouse-model
#6
Jingliang He, Mio Yamane, Shinsuke Shibata, Masaki Fukui, Eisuke Shimizu, Tetsuya Yano, Shin Mukai, Yutaka Kawakami, Shaowei Li, Kazuo Tsubota, Yoko Ogawa
PURPOSE: To report the characteristics of the ocular surface in a previously established sclerodermatous chronic graft-versus-host disease (cGVHD) mouse model. METHODS: The ocular surface features and tear film parameters of the mouse model were assessed by histopathology, immunohistochemistry, electron microscopy, quantitative polymerase chain reaction, and flow cytometry. RESULTS: The mice exhibited loss of body weight and decreased tear secretion (P < 0...
January 16, 2018: Cornea
https://www.readbyqxmd.com/read/29339155/truncated-cd200-stimulates-tumor-immunity-leading-to-fewer-lung-metastases-in-a-novel-wistar-rat-metastasis-model
#7
Jun Kuwabara, Akihiro Umakoshi, Naoki Abe, Yutaro Sumida, Shota Ohsumi, Eika Usa, Kana Taguchi, Mohammed E Choudhury, Hajime Yano, Shirabe Matsumoto, Takeharu Kunieda, Hisaaki Takahashi, Toshihiro Yorozuya, Yuji Watanabe, Junya Tanaka
CD200 mediates immunosuppression in immune cells that express its receptor, CD200R. There are two CD200 variants; truncated CD200 that lacks the N-terminal sequence necessary for CD200R binding (CD200S) and full-length CD200 (CD200L). We established a novel lung metastasis model by subcutaneously transplanting C6 glioma cells into the backs of neonatal Wistar rats. All transplanted rats developed large back tumors, nearly 90% of which bore lung metastases. To compare the effects of CD200S and CD200L on tumor immunity, CD200L (C6-L)- or CD200S (C6-S)-expressing C6 cells were similarly transplanted...
January 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29338084/importance-of-b-cells-to-development-of-tregs-and-prolongation-of-tissue-allograft-survival-in-recipients-receiving-autologous-bone-marrow-transplantation
#8
Reginald M Gorczynski, Kaveh Farrokhi, Christopher Gorczynski, Hassan Sadozai, Fang Zhu, Ismat Khatri
We previously showed that congenic bone marrow transplantation (BMTx) post myeloablation augmented tissue allograft survival in association with increased Tregs of both host and bone marrow donor origin. Regulatory B cells (Bregs) can also modulate T cell immunity and B cells may be implicated in the development of Tregs. Accordingly, we explored the effect of B cell depletion in vivo on augmented graft survival post BMTx. C57BL/6 mice received BALB/c skin allografts followed 7d later by myeloablation using cyclophosphamide and busulphan...
January 16, 2018: Immunology
https://www.readbyqxmd.com/read/29336105/senescence-chips-for-ultrahigh-throughput-isolation-and-removal-of-senescent-cells
#9
Yuchao Chen, Pan Mao, Antoine M Snijders, Daojing Wang
Cellular senescence plays an important role in organismal aging and age-related diseases. However, it is challenging to isolate low numbers of senescent cells from small volumes of biofluids for downstream analysis. Furthermore, there is no technology that could selectively remove senescent cells in a high-throughput manner. In this work, we developed a novel microfluidic chip platform, termed senescence chip, for ultrahigh-throughput isolation and removal of senescent cells. The core component of our senescence chip is a slanted and tunable 3D micropillar array with a variety of shutters in the vertical direction for rapid cell sieving, taking advantage of the characteristic cell size increase during cellular senescence...
January 16, 2018: Aging Cell
https://www.readbyqxmd.com/read/29328479/the-human-umbilical-cord-stem-cells-improve-the-viability-of-oa-degenerated-chondrocytes
#10
Hao Wang, Xu Yan, Yuxin Jiang, Zheng Wang, Yufei Li, Qingdong Shao
Osteoarthritis (OA) affects a large number of patients; however, human umbilical cord stem cells exhibit therapeutic potential for treating OA. The aim of the present study was to explore the interaction between human umbilical cord stem cells and degenerated chondrocytes, and the therapeutic potential of human umbilical cord stem cells on degenerated chondrocytes. Human umbilical cord‑derived mesenchymal stem cells (hUC‑MSCs) were harvested from human umbilical cords, and flow cytometry was used to analyze the surface antigen markers, in addition, chondrogenic, osteogenic and adipogenic differentiation on the cells was investigated...
January 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29328024/endometrial-polyps-in-women-of-reproductive-age-clinical-and-pathogene-tic-variations
#11
N Kosei, N Zakharenko, D Herman
The aim of the study was to study the relationship between the morphofunctional characteristics of the endometrium, hormonal homeostasis and microbiocenosis of the reproductive system in patients with endometrial polyps. The study involved 130 patients aged 18-35 years: 34 patients with endometrial polyps, 30 patients with micropolyps, 36 patients with endometrial polyps and micropolyps, 30 healthy women of the control group. Hysteroscopy was performed for women who had been suspected for endometrial polyps and who had infertility or repeated recurrent miscarriages...
December 2017: Georgian Medical News
https://www.readbyqxmd.com/read/29327714/a-comprehensive-flow-cytometry-based-immunophenotypic-characterization-of-burkitt-like-lymphoma-with-11q-aberration
#12
Grzegorz Rymkiewicz, Beata Grygalewicz, Magdalena Chechlinska, Katarzyna Blachnio, Zbigniew Bystydzienski, Joanna Romejko-Jarosinska, Renata Woroniecka, Michalina Zajdel, Katarzyna Domanska-Czyz, David Martin-Garcia, Ferran Nadeu, Pawel Swoboda, Jolanta Rygier, Barbara Pienkowska-Grela, Jan Konrad Siwicki, Monika Prochorec-Sobieszek, Itziar Salaverria, Reiner Siebert, Jan Walewski
We previously described a subset of MYC translocation-negative aggressive B-cell lymphomas resembling Burkitt lymphoma, characterized by proximal gains and distal losses in chromosome 11. In the 2016 WHO classification, these MYC-negative lymphomas were recognized as a new provisional entity, 'Burkitt-like lymphoma with 11q aberration'. Here we present an immunophenotype analysis of Burkitt-like lymphomas with 11q aberration. Cells were acquired by fine needle aspiration biopsy from 10 young adult patients, 80% of whom presented recurrence-free 5-year survival...
January 12, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29327669/advanced-3d-models-culture-to-investigate-mesenchymal-stromal-cells-of-the-human-dental-follicle
#13
Nathalie Steimberg, Francesca Angiero, Davide Farronato, Angiola Berenzi, Gianguido Cossellu, Andrea Ottonello, Darnell Kaigler, Giovanna Mazzoleni
The human dental follicle (hDF) contains the developing tooth and is involved in regulating tooth maturation and eruption. To investigate the mesenchymal stromal cells of the dental follicle, two 3D culture models were used, based on a dynamic bioreactor: the Rotary Cell Culture System (RCCS) and the 3D culture of precursor cells isolated from follicular tissue (hDFCs). The hDFCs were obtained from impacted third molars of 20 patients. Two 3D culture models were tested. In the first model, intact hDF explants were cultured in 3D conditions, preserving the original tissue architecture; they were studied via histomorphological and molecular analyses...
January 12, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29327082/evaluation-of-committed-and-primitive-cord-blood-progenitors-after-expansion-on-adipose-stromal-cells
#14
E R Andreeva, I V Andrianova, A N Gornostaeva, B Sh Gogiya, L B Buravkova
Umbilical cord blood mononuclear fraction is a valuable source of hematopoietic stem and progenitor cells (CB HSPCs). The rarity of this population is a serious limitation of its application in cell therapy. Ex vivo expansion enables to significantly amplify the number of hematopoietic precursors of different commitment. Here, we expand CB MNCs in co-culture with human adipose tissue-derived stromal cells (ASCs) to enrich HSPCs and describe phenotypic features of newly formed hematopoietic populations. The CD34+-HSPCs demonstrated 6-fold enrichment with 9000 CFUs per 50 × 103 HSPCs on average...
January 11, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29325696/urinary-mrna-analysis-of-biomarkers-to-epithelial-mesenchymal-transition-of-renal-allograft
#15
Imen Mezni, Pierre Galichon, Mohammed Mongi Bacha, Yi-Chun Xu-Dubois, Imen Sfar, David Buob, Sabrina Benbouzid, Rim Goucha, Yousr Gorgi, Ezzedine Abderrahim, Mondher Ounissi, Karine Dahan, Nacera Ouali, Alexandre Hertig, Isabelle Brocheriou, Aly Raies, Taieb Ben Abdallah, Éric Rondeau
Renal allograft loss is most often a chronic process, irrespective of the mechanism at stake. In this prospective study, we studied the expression of epithelial to mesenchymal transition (EMT) markers vimentin and β-catenin by immunohistochemistry in the surveillance biopsy and measured the mRNA encoding vimentin (VIM), CD45, GAPDH and uroplakin 1a (UPK) by quantitative PCR in urinary cells in 75 renal transplant patients. The aim is to establish a simple screening test for chronic renal allograft dysfunction...
January 8, 2018: Néphrologie & Thérapeutique
https://www.readbyqxmd.com/read/29323734/inhibitory-effect-of-circulating-fibrocytes-on-injury-repair-in-acute-lung-injury-acute-respiratory-distress-syndrome-mice-model
#16
Wenlin Tai, Jinyu Li, Boyang Zheng, Hanxin Wu, Jiawei Ding, Ling Gao, Zhaoxin Dong
The study was aimed to explore the functions of circulating fibrocytes (CFs) on injury repair in acute lung injury/acute respiratory distress syndrome (ALI/ARDS) mice model and its clinical value as a biomarker for ALI/ARDS. ALI/ARDS mice model was established by intratracheal instillation of lipopolysaccharide (LPS). Mononuclear cells were isolated from peripheral blood of ALI/ARDS model and flow cytometry was used to measure CFs defined as cells positive for CD45 and collagen-1. Histological changes of lung tissues were evaluated by H&E staining and Masson's trichrome staining...
January 11, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29323717/chondrogenic-differentiation-of-human-scalp-adipose-derived-stem-cells-in-polycaprolactone-scafold-and-using-freeze-thaw-freeze-method
#17
Rana Moradian Tehrani, Hamed Mirzaie, Javad Verdi, Amirhossein Sahebkar, Mahdi Noureddini, Rasoul Salehi, Behrang Alani, Mojtaba Kianmehr
Human adipose tissue has been identified as a viable alternative source for mesenchymal stem cells. SADSCs were isolated from human scalp biopsy and then were characterized by Flow cytometry. SADSCS expressed CD90, CD44, and CD105 but did not express CD45 surface marker. Growth factors were used for chondrogenesis induction. Histology and immunohistology methods and gene expression by real-time PCR 14 days after induced cells have shown the feature of chondrocytes in their morphology and extracellular matrix in both inducing patterns of combination and cycling induction...
January 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29319882/oral-myeloid-cells-uptake-allergoids-coupled-to-mannan-driving-th1-treg-responses-upon-sublingual-delivery-in-mice
#18
Irene Soria, Juan López-Relaño, Marcos Viñuela, José-Ignacio Tudela, Alba Angelina, Cristina Benito-Villalvilla, Carmen M Díez-Rivero, Bárbara Cases, Ana I Manzano, Enrique Fernández-Caldas, Miguel Casanovas, Oscar Palomares, José L Subiza
BACKGROUND: Polymerized allergoids coupled to nonoxidized mannan (PM-allergoids) may represent novel vaccines targeting dendritic cells (DCs). PM-allergoids are better captured by DCs than native allergens and favor Th1/Treg cell responses upon subcutaneous injection. Herein we have studied in mice the in vivo immunogenicity of PM-allergoids administered sublingually in comparison to native allergens. METHODS: Three immunization protocols (4-8 weeks long) were used in Balb/c mice...
January 10, 2018: Allergy
https://www.readbyqxmd.com/read/29316245/flow-cytometric-aberrancies-in-plasma-cell-myeloma-and-mgus-correlation-with-laboratory-parameters
#19
Sarika Gupta, Nitin J Karandikar, Timothy Ginader, Andrew M Bellizzi, Carol J Holman
BACKGROUND: Multiparametric flow cytometry (MFC) is a useful tool for diagnosis of plasma cell dyscrasias and assessment of minimal residual disease (MRD) in plasma cell myeloma (PCM). However, the immunophenotypic differences between the clonal plasma cells (PCs) of plasma cell myeloma (PCM) and those of monoclonal gammopathy of undetermined significance (MGUS) as well as the correlation of these flow cytometric markers with pertinent laboratory parameters have not been evaluated. METHODS: We retrospectively identified all newly diagnosed treatment-naive PCM and MGUS patients between 09/2014 and 06/2015 who underwent 10-color flow-cytometric evaluation: CD45, CD38, CD138, cKappa, cLambda, CD19, CD27, CD28, CD56, CD117...
January 6, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29316178/evaluation-of-cd229-as-a-new-alternative-plasma-cell-gating-marker-in-the-flow-cytometric-immunophenotyping-of-monoclonal-gammopathies
#20
Prashant R Tembhare, Sitaram Ghogale, Wilma Tauro, Yajamanam Badrinath, Nilesh Deshpande, Shweta Kedia, Keziah Cherian, Nikhil V Patkar, Gaurav Chatterjee, Sumeet Gujral, Papagudi G Subramanian
BACKGROUND: Current flow-cytometric plasma cell (PC) gating is based on CD138, CD38 and CD45 expression. CD138 is known for variable expression and loss during storage and processing. Introduction of anti-CD38 and anti-CD138 monoclonal-antibody therapies has limited the use of these markers during follow-up. Hence, additional reliable PC-gating markers are required. Recently, CD229 has been claimed as an alternative PC-gating marker. However, these studies are limited to a small cohort of samples...
January 9, 2018: Cytometry. Part B, Clinical Cytometry
keyword
keyword
95966
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"