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Acute fever igm dengue serology viral isolation

Camila Zanluca, Giovanny Augusto Camacho Antevere Mazzarotto, Juliano Bordignon, Claudia Nunes Duarte Dos Santos
Dengue is the most prevalent human arboviral disease. The morbidity related to dengue infection supports the need for an early, quick and effective diagnostic test. Brazil is a hotspot for dengue, but no serological diagnostic test has been produced using Brazilian dengue virus isolates. This study aims to improve the development of immunodiagnostic methods for dengue virus (DENV) detection through the production and characterization of 22 monoclonal antibodies (mAbs) against Brazilian isolates of DENV-1, -2 and -3...
2014: PloS One
Mamidi Neeraja, Vemu Lakshmi, P K Dash, M M Parida, P V L Rao
INTRODUCTION: Dengue is an acute viral infection which presents as uneventful pyrexia to a fatal complication. This infection is increasingly being recognized as the world's major emerging tropical disease and an important public health problem. This article highlights the clinical manifestations of Dengue virus infection and the various molecular tests that were used for its laboratory diagnosis. METHODS: Serum samples from 713 suspected cases of Dengue were collected between August and December 2007...
March 2013: Journal of Clinical and Diagnostic Research: JCDR
Megan E Reller, Champika Bodinayake, Ajith Nagahawatte, Vasantha Devasiri, Wasantha Kodikara-Arachichi, John J Strouse, Anne Broadwater, Truls Østbye, Aravinda de Silva, Christopher W Woods
Dengue virus (DENV), a globally emerging cause of undifferentiated fever, has been documented in the heavily urbanized western coast of Sri Lanka since the 1960s. New areas of Sri Lanka are now being affected, and the reported number and severity of cases have increased. To study emerging DENV in southern Sri Lanka, we obtained epidemiologic and clinical data and acute- and convalescent-phase serum samples from patients >2 years old with febrile illness. We tested paired serum samples for DENV IgG and IgM and serotyped virus by using isolation and reverse transcription PCR...
February 2012: Emerging Infectious Diseases
Ruth Aralí Martínez-Vega, Fredi Alexander Díaz-Quijano, Carolina Coronel-Ruiz, Sergio Yebrail Gómez, Luis Angel Villar-Centeno
INTRODUCTION: Traditional tests for dengue diagnosis are not useful during the first days of disease onset, because they require time and complex techniques or because they have low sensitivity. Objective. The reliability and precision was determined for the rapid test in cassette (PANBIO) for dengue diagnosis in acute and convalescent serum samples. MATERIALS AND METHODS: The immunochromatographic test was evaluated in samples from patients older than 5 years with acute febrile syndrome...
December 2009: Biomédica: Revista del Instituto Nacional de Salud
Apiradee Theamboonlers, Pornpimol Rianthavorn, Kesmanee Praianantathavorn, Norra Wuttirattanakowit, Yong Poovorawan
In 2008, an outbreak of chikungunya virus (CHIKV) occurred in Narathiwat province, south Thailand. To determine the clinical significance, molecular epidemiology and evolutionary origin of the CHIKV causing this outbreak, 47 patients who had been admitted to Narathiwatratchanakharin provincial hospital due to acute febrile illness were enrolled in this study. Sera were tested for IgM antibodies, and RT-PCR was performed for CHIKV and dengue virus. We diagnosed 10 patients with CHIKV infection and 5 with dengue virus infection...
July 2009: Japanese Journal of Infectious Diseases
Ruth Aralí Martínez-Vega, Fredi Alexander Díaz-Quijano, Luis Angel Villar-Centeno
BACKGROUND: Early detection of dengue could help to prevent its complications. The usefulness of clinical criteria for diagnosis of the disease must be ascertained. AIMS: To assess the correlation between laboratory and clinical diagnosis of dengue, done during the first consultation in the emergency room. To estimate the impact of clinical diagnosis on the initial medical treatment. PATIENTS AND METHODS: Patients older than 5 years with an acute febrile syndrome that consulted during the first 72 hours of disease, during 2004, at an emergency room in Bucaramanga, Colombia, were studied...
September 2006: Revista Médica de Chile
R K Ratho, B Mishra, J Kaur, N Kakkar, K Sharma
BACKGROUND: Dengue viral infection is one of the most important public health problem in tropical countries. AIM: An outbreak of dengue fever was investigated in a periurban slum area of Chandigarh, India, during September to December, 2002. MATERIALS AND METHODS: Blood samples from 218 patients and 30 apparently healthy contacts were tested for dengue-specific immunoglobulin M (IgM) and IgG antibodies including 80 acute samples collected within 5 days of illness were subjected for virus isolation in newborn mice...
December 2005: Indian Journal of Medical Sciences
Philippe Buchy, Van Luong Vo, Khanh Toan Bui, Thi Xuan Mai Trinh, Philippe Glaziou, Thi Thu Ha Le, Viet Lo Le, Trong Chien Bui
This study was designated to describe clinical and biological features of patients with a suspected diagnosis of dengue fever/dengue hemorrhagic fever during an outbreak in Central Vietnam. One hundred and twenty-five consecutive patients hospitalized at Khanh Hoa and Binh Thuan Provincial hospitals between November 2001 and January 2002 with a diagnosis of suspected dengue infection were included in the present study. Viruses were isolated in C6/36 and VERO E6 cell cultures or detected by RT-PCR. A hemagglutination-inhibition test (HI) was done on each paired sera using dengue antigens type 1-4, Japanese encephalitis (JE) virus antigen, Chickungunya virus antigen and Sindbis virus antigen...
January 2005: Southeast Asian Journal of Tropical Medicine and Public Health
Nono C Sukri, Kanti Laras, Toni Wandra, Sukman Didi, Ria P Larasati, Josef R Rachdyatmaka, Stevie Osok, Petrus Tjia, John M Saragih, Sri Hartati, Erlin Listyaningsih, Kevin R Porter, Charmagne G Beckett, Ingerani S Prawira, Narain Punjabi, Sri A Suparmanto, H James Beecham, Michael J Bangs, Andrew L Corwin
In April 2001, a second suspected outbreak of dengue hemorrhagic fever in the easternmost region of Indonesia was investigated in Merauke, a town located in the southeastern corner of Papua, by the Indonesian Ministry of Health and the U.S. Naval Medical Research Unit No. 2. Principal case criteria of hemorrhagic disease provided for a study enrollment of 15 clinically acute and 37 convalescing subjects. Additionally, 32 comparable age/sex controls were selected from neighboring households. Laboratory diagnosis involved three testing methodologies: virus isolation by cell culture, a reverse transcriptase-polymerase chain reaction (RT-PCR) assay, and serologic assays...
May 2003: American Journal of Tropical Medicine and Hygiene
M Simmons, K R Porter, J Escamilla, R Graham, D M Watts, K H Eckels, C G Hayes
To increase the specificity of dengue (DEN) diagnosis based on antibody detection, we have evaluated recombinant proteins as antigens that incorporate most of the B domain of the DEN virus envelope protein fused to the trpE protein of Escherichia coli (trpE-DEN). A pooled antigen consisting of trpE-DEN proteins representing all four serotypes of DEN virus was used in an indirect ELISA for the detection of IgG or IgM antibody. This assay was compared with a standard IgG indirect ELISA and an IgM-capture ELISA using DEN virus-infected cell culture pooled antigens...
February 1998: American Journal of Tropical Medicine and Hygiene
D W Vaughn, S Green, S Kalayanarooj, B L Innis, S Nimmannitya, S Suntayakorn, A L Rothman, F A Ennis, A Nisalak
A multicenter effort was begun in 1994 to characterize the pathophysiology of dengue using a study design that minimized patient selection bias by offering enrollment to all children with undifferentiated fever for <72 h. In the first year, 189 children were enrolled (age range, 8 months to 14 years). Thirty-two percent of these children had dengue infections (60 volunteers). The percentage of children with a secondary dengue infection was 93%, with only 4 (7%) having a primary dengue infection. The virus isolation rate from the plasma of children with dengue was 98%...
August 1997: Journal of Infectious Diseases
A F Trofa, R F DeFraites, B L Smoak, N Kanesa-thasan, A D King, J M Burrous, P O MacArthy, C Rossi, C H Hoke
OBJECTIVE: To describe clinical manifestations and public health implications of an outbreak of dengue fever (DF) during Operation Uphold Democracy, Haiti, 1994. DESIGN: Consecutive sample. SETTING: Military combat support hospital, Port-au-Prince, Haiti. PATIENTS: A total of 101 US military personnel with acute febrile illnesses. INTERVENTIONS: A disease surveillance team collected clinical and epidemiologic data from US military clinics throughout Haiti...
May 21, 1997: JAMA: the Journal of the American Medical Association
G R Rodier, D J Gubler, S E Cope, C B Cropp, A K Soliman, D Polycarpe, M A Abdourhaman, J P Parra, J Maslin, R R Arthur
From October 1991 to February 1992, an outbreak of acute fever (in which thick blood films were negative for malaria) spread rapidly in the city of Djibouti, Djibouti Republic, affecting all age groups and both nationals and foreigners. The estimated number of cases was 12,000. The clinical features were consistent with a non-haemorrhagic dengue-like illness. Serum samples from 91 patients were analysed serologically for flavivirus infection (dengue 1-4, West Nile, yellow fever, Zika, Banzi, and Uganda-S), and virus isolation was attempted...
May 1996: Transactions of the Royal Society of Tropical Medicine and Hygiene
J G Rigau-Pérez, D J Gubler, A V Vorndam, G G Clark
PROBLEM/CONDITION: Dengue is an acute, mosquito-transmitted viral disease characterized by fever, headache, arthralgia, myalgia, rash, nausea, and vomiting. The worldwide incidence of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) increased from the mid-1970s through 1992. Although dengue is not endemic to the 50 United States, it presents a risk to U.S. residents who visit dengue-endemic areas. REPORTING PERIOD COVERED: 1986-1992. DESCRIPTION OF SYSTEM: Dengue surveillance in the 50 United States and the U...
July 22, 1994: MMWR. CDC Surveillance Summaries: Morbidity and Mortality Weekly Report. CDC Surveillance Summaries
B L Innis, A Nisalak, S Nimmannitya, S Kusalerdchariya, V Chongswasdi, S Suntayakorn, P Puttisri, C H Hoke
The diagnostic sensitivity and specificity of detection of anti-dengue IgM by antibody capture enzyme-linked immunosorbent assay (ELISA) was investigated in dengue infections in a variety of clinical settings. Sera from uninfected controls were uniformly negative. Serial specimens from experimental and natural infections showed that viremia and fever terminated as anti-dengue IgM became detectable. Anti-dengue IgM appeared in most cases by the 3rd afebrile day of illness and declined to undetectable levels after 30-60 days...
April 1989: American Journal of Tropical Medicine and Hygiene
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