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https://www.readbyqxmd.com/read/29455276/methylation-of-secreted-frizzled-related-protein-1-sfrp1-promoter-downregulates-wnt-%C3%AE-catenin-activity-in-keloids
#1
Jiaqi Liu, Huayu Zhu, Hongtao Wang, Jun Li, Fu Han, Yang Liu, Wanfu Zhang, Ting He, Na Li, Zhao Zheng, Dahai Hu
Keloid, a benign skin disorder, forms during wound healing in genetically susceptible individuals. To better control keloid and understand the molecular mechanisms, this study screened gene hypermethylations of GEO database microarray data on keloids and identified the hypermethylation of the secreted frizzled related protein-1 (SFRP1) promoter. Subsequently, hypermethylation and mRNA and protein levels were assessed in 57 cases of keloid vs. normal skin tissues. Fibroblasts from tissues were isolated for the assessment of gene regulation in vitro...
February 17, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29443079/gene-regulation-and-targeted-therapy-in-gastric-cancer-peritoneal-metastasis-radiological-findings-from-dual-energy-ct-and-pet-ct
#2
Bowen Shi, Huimin Lin, Miao Zhang, Wei Lu, Ying Qu, Huan Zhang
Gastric cancer remains fourth in cancer incidence worldwide with a five-year survival of only 20%-30%. Peritoneal metastasis is the most frequent type of metastasis that accompanies unresectable gastric cancer and is a definitive determinant of prognosis. Preventing and controlling the development of peritoneal metastasis could play a role in helping to prolong the survival of gastric cancer patients. A non-invasive and efficient imaging technique will help us to identify the invasion and metastasis process of peritoneal metastasis and to monitor the changes in tumor nodules in response to treatments...
January 22, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29391876/antagonism-between-hedgehog-and-wnt-signaling-pathways-regulates-tumorigenicity
#3
Mei Ding, Xin Wang
The crosstalk of multiple cellular signaling pathways is crucial in animal development and tissue homeostasis, and its dysregulation may result in tumor formation and metastasis. The Hedgehog (Hh) and Wnt signaling pathways are both considered to be essential regulators of cell proliferation, differentiation and oncogenesis. Recent studies have indicated that the Hh and Wnt signaling pathways are closely associated and involved in regulating embryogenesis and cellular differentiation. Hh signaling acts upstream of the Wnt signaling pathway, and negative regulates Wnt activity via secreted frizzled-related protein 1 (SFRP1), and the Wnt/β-catenin pathway downregulates Hh activity through glioma-associated oncogene homolog 3 transcriptional regulation...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29381095/in-vitro-effects-of-secreted-frizzled-related-protein-1-sfrp1-on-human-corneal-epithelial-cells
#4
Jingjing You, Luis Munoz-Erazo, Li Wen, Chris Hodge, Michele C Madigan, Gerard Sutton
PURPOSE: Limbal corneal epithelial cells (LCECs) are responsible for corneal epithelial cell regeneration. However, corneal central epithelial cells (CCECs) are also suggested to display potential for self-renewal. Additionally, a better understanding of molecules that regulate corneal epithelial cell regeneration is important for studying conditions affecting the cornea, for example, keratoconus. Given our previous findings of reduced levels of secreted frizzled-related protein 1 (SFRP1) in tears from keratoconus patients compared to controls, we investigated the effects of SFRP1 on the proliferation and survival of cultured central and limbal human corneal epithelial cells...
January 30, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29374860/blood-collection-and-cell-free-dna-isolation-methods-influence-the-sensitivity-of-liquid-biopsy-analysis-for-colorectal-cancer-detection
#5
Barbara Kinga Barták, Alexandra Kalmár, Orsolya Galamb, Barnabás Wichmann, Zsófia Brigitta Nagy, Zsolt Tulassay, Magdolna Dank, Péter Igaz, Béla Molnár
During colorectal cancer (CRC) development tumor-derived cell-free DNA (cfDNA) can be released into the bloodstream. Many different cfDNA isolation methods and specific blood collection tubes preventing the release of genomic DNA and stabilizing cfDNA with preservative reagents became available. These factors may affect greatly on the further liquid biopsy analyses. Our aim was to test different blood collection tubes and cfDNA isolation methods to determine whether these factors influence the cfDNA amount and the promoter methylation of four previously described hypermethylated biomarkers...
January 27, 2018: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/29371858/bioinformatics-based-identification-of-mir-542-5p-as-a-predictive-biomarker-in-breast-cancer-therapy
#6
Qiong-Ni Zhu, Helen Renaud, Ying Guo
Background: Tamoxifen is the first-line hormone therapy for estrogen receptor alpha positive (ERα+) breast cancer. However, about 40% of patients with ERα + breast cancer who receive tamoxifen therapy eventually develop resistance resulting in a poor prognosis. The aim of this study was to mine available data sets in the Gene Expression Omnibus (GEO) database, including in vitro (cell lines) and in vivo (tissue samples), and to identify all miRNAs associated with tamoxifen resistance (TamR) in breast cancer...
2018: Hereditas
https://www.readbyqxmd.com/read/29358129/mir-1301-3p-promotes-prostate-cancer-stem-cell-expansion-by-targeting-sfrp1-and-gsk3%C3%AE
#7
Xian-Lu Song, Bin Huang, Bo-Wei Zhou, Chong Wang, Zhi-Wei Liao, Yi Yu, Shan-Chao Zhao
Cancer stem cells promote tumor progression, drug-resistance, and relapse, and many microRNAs (miRNAs) play critical roles in the expansion of cancer stem cells. In the present study, we investigated the role of miR-1301-3p in the expansion of prostate cancer stem cells; miR-1301-3p was significantly upregulated in prostate cancer cells and tissues compared with normal prostate cells and tissues. Sphere formation and side population assays suggested that miR-1301-3p promoted the expansion of prostate cancer stem cells, and increased the expression of prostate cancer stem cell-associated genes, such as OCT4, SOX2, NANOG, CD44, KLF4, c-MYC, and MMP2...
January 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29319176/mir-542-3p-prevents-ovariectomy-induced-osteoporosis-in-rats-via-targeting-sfrp1
#8
Xiguang Zhang, Yun Zhu, Chuanlin Zhang, Jianping Liu, Tianming Sun, Dan Li, Qiang Na, Zhaowei Teng
Secreted frizzled-related protein-1 (SFRP1) is a negative regulatory molecule of the WNT signaling pathway and serves as a therapeutic target for bone formation in osteoporosis. In this study, we first established an ovariectomized (OVX) rat model to simulate postmenopausal osteoporosis and found significant changes in miR-542-3p and sFRP1 expression by RNA sequencing and qRT-PCR. In addition, there was a significant negative correlation between miR-542-3p and sFRP1 mRNA levels in postmenopausal women with osteoporosis...
January 10, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29258605/mir-105-93-3p-promotes-chemoresistance-and-circulating-mir-105-93-3p-acts-as-a-diagnostic-biomarker-for-triple-negative-breast-cancer
#9
Hao-Yi Li, Jui-Lin Liang, Yao-Lung Kuo, Hao-Hsien Lee, Marcus J Calkins, Hong-Tai Chang, Forn-Chia Lin, Yu-Chia Chen, Tai-I Hsu, Michael Hsiao, Luo-Ping Ger, Pei-Jung Lu
BACKGROUND: Triple negative breast cancer (TNBC) lacks both early detection biomarkers and viable targeted therapeutics. Moreover, chemotherapy only produces 20-30% pathologic complete response. Because miRNAs are frequently dysregulated in breast cancer and have broad tissue effects, individual or combinations of circulating miRNAs may serve as ideal diagnostic, predictive or prognostic biomarkers, as well as therapeutic targets. Understanding the role and mechanism of dysregulated miRNAs in TNBC may help to develop novel diagnostic and prognostic strategy for TNBC patients...
December 19, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29215727/secreted-frizzled-related-protein-1-regulates-the-progression-of-neuropathic-pain-in-mice-following-spinal-nerve-ligation
#10
Jun Tang, Qing Ji, Li Jin, Mi Tian, Li Dong Zhang, Xiao Yu Liu
Previous studies have showed that the Wnt/β-catenin signaling pathway plays an important role in modulating neuropathic pain after sciatic nerve injury. In this study, we explored the role of secreted frizzled-related protein 1 (SFRP1), a Wnt antagonist, in neuropathic pain using a rat model following spinal nerve ligation (SNL). We found SNL-induced SFRP1 downregulation in the spinal cord. Further, the overexpression of SFRP1 via spinal injection into the spinal cord attenuated SNL-induced allodynia, hyperalgesia and neuroinflammation...
December 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29212200/cell-free-dna-promoter-hypermethylation-in-plasma-as-a-predictive-marker-for-survival-of-patients-with-pancreatic-adenocarcinoma
#11
Stine Dam Henriksen, Poul Henning Madsen, Anders Christian Larsen, Martin Berg Johansen, Inge Søkilde Pedersen, Henrik Krarup, Ole Thorlacius-Ussing
Introduction: Few prognostic biomarkers are available for pancreatic cancer. The aim of this study is to examine the correlation between the survival of pancreatic adenocarcinoma patients and hypermethylated genes in plasma-derived cell-free DNA. Methods: Consecutive patients with pancreatic adenocarcinoma were prospectively included and staged according to the TNM classification. Methylation-specific PCR of 28 genes was conducted. A survival prediction model independent of cancer stage and stage-specific survival prediction models were developed by multivariable Cox regression analysis using backward stepwise selection...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29196093/sonic-hedgehog-regulates-the-pathfinding-of-descending-serotonergic-axons-in-hindbrain-in-collaboration-with-wnt5a-and-secreted-frizzled-related-protein-1
#12
Jie Xie, Teng Zhao, Yaobo Liu
Previous studies have demonstrated that both Wnt5a and Sonic hedgehog (Shh) are involved in regulating the pathfinding of descending serotonergic (5-HT, 5-hydroxytryptamine) axons in an opposite manner in the brainstem. Shh and Wnt signaling pathways interact to guide post-crossing commissural axons, where Shh acts as a repellent directly and shaping the Wnt gradient indirectly by regulating the gradient expression of the frizzled-related protein 1 (Sfrp1). Whether such a mechanism functions in descending 5-HT axon guidance remains unknown...
November 28, 2017: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/29146058/methylation-associated-silencing-of-sfrp1-gene-in-high-grade-serous-ovarian-carcinomas
#13
Vedran Kardum, Valentina Karin, Mislav Glibo, Anita Skrtic, Tamara Nikuseva Martic, Nermina Ibisevic, Faruk Skenderi, Semir Vranic, Ljiljana Serman
Wnt is a highly conserved signaling pathway responsible for tissue regeneration, maintenance and differentiation of stem cells in adults. Its aberrant activation through reduced expression of Wnt signaling pathway inhibitors, such as proteins from the SFRP family, is commonly seen in many tumors. In the present study we explored SFRP1 protein expression using immunohistochemistry in 11 low-grade serous ovarian carcinomas (LGSC), 42 high-grade serous ovarian carcinomas (HGSC), and 5 normal ovarian tissues (controls)...
December 2017: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/29129759/dampening-of-the-bone-formation-response-following-repeat-dosing-with-sclerostin-antibody-in-mice-is-associated-with-up-regulation-of-wnt-antagonists
#14
Gill Holdsworth, Kevin Greenslade, Joby Jose, Zofia Stencel, Hishani Kirby, Adrian Moore, Hua Zhu Ke, Martyn K Robinson
Administration of antibodies to sclerostin (Scl-Ab) has been shown to increase bone mass, bone mineral density (BMD) and bone strength by increasing bone formation and decreasing bone resorption in both animal studies and human clinical trials. In these studies, the magnitude and rate of increase in bone formation markers is attenuated upon repeat dosing with Scl-Ab despite a continuous and progressive increase in BMD. Here, we investigated whether the attenuation in the bone formation response following repeated administration of Scl-Ab was associated with increased expression of secreted antagonists of Wnt signalling and determined how the circulating marker of bone formation, P1NP, responded to single, or multiple doses, of Scl-Ab four days post-dosing...
November 9, 2017: Bone
https://www.readbyqxmd.com/read/29128220/identification-of-potential-embryokines-in-the-bovine-reproductive-tract
#15
P Tríbulo, L G B Siqueira, L J Oliveira, T Scheffler, P J Hansen
Knowledge of the molecules used by the maternal reproductive tract to regulate development of the preimplantation embryo is largely incomplete. The goal of the present experiment was to identify candidates for this function. The approach was to assess expression patterns in the endometrium and oviduct of 93 genes encoding for hormones, growth factors, chemokines, cytokines, and WNT-related molecules. Results show that all of the genes were expressed in the reproductive tract. Expression in oviduct was affected by day of the estrous cycle for 21 genes with 11 genes having highest expression at estrus (CCL21, CTGF, CXCL10, CXCL16, DKK3, FGF10, IL18, IL33, IL34, PGF, and SFRP2), one gene at d 3 (WNT4), 8 at d 5 (BMP7, HGF, IL6, SFRP1, TGFB1, WIF1, WNT2, and WNT5A), and one at d 7 (IK)...
November 8, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28984209/genome-wide-identification-of-key-modulators-of-gene-gene-interaction-networks-in-breast-cancer
#16
Yu-Chiao Chiu, Li-Ju Wang, Tzu-Hung Hsiao, Eric Y Chuang, Yidong Chen
BACKGROUND: With the advances in high-throughput gene profiling technologies, a large volume of gene interaction maps has been constructed. A higher-level layer of gene-gene interaction, namely modulate gene interaction, is composed of gene pairs of which interaction strengths are modulated by (i.e., dependent on) the expression level of a key modulator gene. Systematic investigations into the modulation by estrogen receptor (ER), the best-known modulator gene, have revealed the functional and prognostic significance in breast cancer...
October 3, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28962550/a-transcriptome-based-protein-network-that-identifies-new-therapeutic-targets-in-colorectal-cancer
#17
Stéphanie Durand, Killian Trillet, Arnaud Uguen, Aude Saint-Pierre, Catherine Le Jossic-Corcos, Laurent Corcos
BACKGROUND: Colon cancer occurrence is increasing worldwide, making it the third most frequent cancer. Although many therapeutic options are available and quite efficient at the early stages, survival is strongly decreased when the disease has spread to other organs. The identification of molecular markers of colon cancer is likely to help understanding its course and, eventually, to uncover novel genes to be targeted by drugs. In this study, we compared gene expression in a set of 95 human colon cancer samples to that in 19 normal colon mucosae, focusing on 401 genes from 5 selected pathways (Apoptosis, Cancer, Cholesterol metabolism and lipoprotein signaling, Drug metabolism, Wnt/beta-catenin)...
September 30, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28947991/plasma-protein-profiling-in-patients-undergoing-coronary-artery-bypass-grafting-surgery-and-clinical-significance
#18
Zhi-Peng Guo, Hai-Tao Hou, Rui Jing, Zhen-Guo Song, Xiao-Cheng Liu, Guo-Wei He
This study was designed to identify the protein profiling in patients with triple vessel coronary artery disease (CAD) undergoing CABG, in order to detect CAD-related differential proteins in these patients. CABG patients with triple vessel disease with/without left main stenosis (n =160) were compared to normal coronary angiographic subjects (n =160). Plasma samples of 20 males and 20 females in each group were analyzed with iTRAQ technique. ELISA test was used to test the chosen proteins from iTRAQ results in plasma samples from a new cohort of the CABG group (n=120, male/femal=61/59) and control (n =120, male/female=60/60)...
September 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28894084/tbx3-dependent-amplifying-stem-cell-progeny-drives-interfollicular-epidermal-expansion-during-pregnancy-and-regeneration
#19
Ryo Ichijo, Hiroki Kobayashi, Saori Yoneda, Yui Iizuka, Hirokazu Kubo, Shigeru Matsumura, Satsuki Kitano, Hitoshi Miyachi, Tetsuya Honda, Fumiko Toyoshima
The skin surface area varies flexibly in response to body shape changes. Skin homeostasis is maintained by stem cells residing in the basal layer of the interfollicular epidermis. However, how the interfollicular epidermal stem cells response to physiological body shape changes remains elusive. Here, we identify a highly proliferative interfollicular epidermal basal cell population in the rapidly expanding abdominal skin of pregnant mice. These cells express Tbx3 that is necessary for their propagation to drive skin expansion...
September 11, 2017: Nature Communications
https://www.readbyqxmd.com/read/28837545/il-17a-inhibits-osteogenic-differentiation-of-bone-mesenchymal-stem-cells-via-wnt-signaling-pathway
#20
Zhenguo Wang, Ying Jia, Fu Du, Min Chen, Xiuhua Dong, Yan Chen, Wen Huang
BACKGROUND Interleukin-17A (IL-17A) is not only an important modulator of inflammatory reactions, but also affects bone metabolism, which is involved in osteogenic differentiation of stem cells. However, the role and mechanism of IL-17A in osteogenic differentiation of bone mesenchymal stem cells (BMSCs) are not fully understood. In this study, we investigated the role and mechanism of IL-17A in osteogenic differentiation of BMSCs. MATERIAL AND METHODS The osteogenic differentiation of BMSCs was induced by osteoblast-induction medium with IL-17A or without IL-17A...
August 24, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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