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https://www.readbyqxmd.com/read/29665441/nanorod-diameter-modulated-osteogenic-activity-of-hierarchical-micropore-nanorod-patterned-coatings-via-a-wnt-%C3%AE-catenin-pathway
#1
Jianhong Zhou, Lingzhou Zhao, Bo Li, Yong Han
Hierarchical micropore/nanorod-patterned strontium doped hydroxyapatite (Ca9 Sr1 (PO4 )6 (OH)2 , Sr1 -HA) structures (MNRs) with different nanorod diameters of about 30, 70 and 150 nm were coated on titanium, to investigate the effect of nanorod diameter on osteogenesis and the involved mechanism. Compared to micropore/nanogranule-patterned Sr1 -HA coating (MNG), MNRs gave rise to dramatically enhanced in vitro mesenchymal stem cell functions including osteogenic differentiation in the absence of osteogenic supplements and in vivo osseointegration related to the nanorod diameter with about 70 nm displaying the best effects...
April 14, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29625497/impact-of-the-canonical-wnt-pathway-activation-on-the-pathogenesis-and-prognosis-of-adamantinomatous-craniopharyngiomas
#2
Carlos Eduardo Barros Jucá, Leandro Machado Colli, Clarissa Silva Martins, Marina Lanciotti Campanini, Beatriz Paixão, Renata Viana Jucá, Fabiano Pinto Saggioro, Ricardo Santos de Oliveira, Ayrton Custódio Moreira, Hélio Rubens Machado, Luciano Neder, Sonir Rauber Antonini, Margaret de Castro
CTNNB1 mutations and abnormal β-catenin distribution are associated with the pathogenesis of adamantinomatous craniopharyngioma (aCP). We evaluated the expression of the canonical Wnt pathway components in aCPs and its association with CTNNB1 mutations and tumor progression. Tumor samples from 14 aCP patients and normal anterior pituitary samples from eight individuals without pituitary disease were studied. Gene expression of Wnt pathway activator ( WNT4 ), inhibitors ( SFRP1 , DKK3 , AXIN1 , and APC ), transcriptional activator ( TCF7 ), target genes ( MYC , WISP2 , and, CDH1 ), and Wnt modulator ( TP53 ) was evaluated by qPCR...
April 6, 2018: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/29625468/the-role-of-wnt-%C3%AE-catenin-signaling-and-k-cadherin-in-the-regulation-of-intraocular-pressure
#3
Hannah C Webber, Jaclyn Y Bermudez, J Cameron Millar, Weiming Mao, Abbot F Clark
Purpose: Wnt/β-catenin signaling in the trabecular meshwork (TM) is required for maintaining normal intraocular pressure (IOP), although the mechanism(s) behind this are unknown. We hypothesize that Wnt/β-catenin signaling regulates IOP via β-catenin's effects on cadherin junctions. Methods: Nonglaucomatous primary human TM (NTM) cells were treated with or without 100 ng/ml Wnt3a, 1 μg/ml sFRP1, or both for 4 to 48 hours. Cells were immunostained for β-catenin, total cadherins, or cadherin isoforms...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29610564/effects-of-secreted-frizzled-related-protein-1-on-proliferation-migration-invasion-and-apoptosis-of-colorectal-cancer-cells
#4
Zhongchuan Wang, Rujia Li, Yongshan He, Shiyong Huang
Background: Secreted frizzled-related protein 1 (SFRP1) is a member of the SFRPs family that modulates the Wnt signal transduction pathway. Recent studies have showed down-regulation of SFRP1 expression in colorectal cancer (CRC). We aimed to evaluate the effect of SFRP1 on the proliferation, migration, invasion and apoptosis of CRC cells in vitro. Materials and methods: We used real-time fluorescence quantification (RT-PCR) and Western blotting to detect SFRP1 expression in CRC, pericarcinomatous tissues and CRC cell lines...
2018: Cancer Cell International
https://www.readbyqxmd.com/read/29601907/inhibition-of-dnmt-suppresses-the-stemness-of-colorectal-cancer-cells-through-down-regulating-wnt-signaling-pathway
#5
Shanxin Li, Zhipeng Han, Naping Zhao, Bing Zhu, Qianwen Zhang, Xue Yang, Dandan Sheng, Jing Hou, Shiwei Guo, Lixin Wei, Li Zhang
Cancer stem cell (CSC) theory reveals a new insight into the understanding of tumorigenesis and metastasis. Recently, DNA methylation is suggested to be a potential epigenetic mechanism for maintenance of CSCs. What's more, studies have shown that DNA methyltransferase (DNMT) is essential for CSCs and deletion of DNMT can reduce tumorigenesis by limiting CSC pool. Therefore, targeting the epigenetic modifiers especially DNA methylation offers an optional strategy for treating human cancers. In the present study we found that DNMT inhibitor 5-Aza-2'-deoxycytidine (5-AzaDC) markedly reduced colorectal CSC abundance in vitro and suppressed liver metastatic tumor growth in vivo...
March 27, 2018: Cellular Signalling
https://www.readbyqxmd.com/read/29579399/gsk3-activated-stat5-regulates-expression-of-sfrps-to-modulate-adipogenesis
#6
Li Wang, Yuan Wang, Yuan Meng, Chao Zhang, Lijun Di
Adipogenesis is a tightly regulated cellular process and is closely associated with obesity and its associated metabolic disorders, such as diabetes. Multiple transcription factors and signaling pathways are involved in the regulation of adipogenesis. Here, we report that glycogen synthase kinase (GSK3), which was reported to play an important role in many cellular processes, is essential to adipocyte differentiation at early and terminal differentiation phases. Mechanistically, GSK3 modulates adipogenesis through regulation of both canonical Wnt pathways, which involve Wnt/β-catenin signaling, and noncanonical Wnt pathways, which include JNK and Ras-related C3 botulinum toxin substrate signaling...
March 26, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29559248/role-of-mir-452-5p-in-the-tumorigenesis-of-prostate-cancer-a-study-based-on-the-cancer-genome-atl-tcga-gene-expression-omnibus-geo-and-bioinformatics-analysis
#7
Li Gao, Li-Jie Zhang, Sheng-Hua Li, Li-Li Wei, Bin Luo, Rong-Quan He, Shuang Xia
BACKGROUND: MiR-452-5p has been reported to be down-regulated in prostate cancer, affecting the development of this type of cancer. However, the molecular mechanism of miR-452-5p in prostate cancer remains unclear. Therefore, we investigated the network of target genes of miR-452-5p in prostate cancer using bioinformatics analyses. MATERIALS AND METHODS: We first analyzed the expression profiles and prognostic value of miR-452-5p in prostate cancer tissues from a public database...
March 6, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29524208/loss-of-ffar2-promotes-colon-cancer-by-epigenetic-dysregulation-of-inflammation-suppressors
#8
Pan Pan, Kiyoko Oshima, Yi-Wen Huang, Kimberle A Agle, William R Drobyski, Xiao Chen, Jianying Zhang, Martha M Yearsley, Jianhua Yu, Li-Shu Wang
Free fatty acid receptor 2 (FFAR2, also named GPR43), is activated by short-chain fatty acids (SCFAs), such as butyrate, that are produced when gut bacteria ferment dietary fiber. FFAR2 has been suggested to regulate colonic inflammation, which is a major risk factor for the development of colon cancer and is also linked to epigenetic dysregulation in colon carcinogenesis. The current study assessed whether FFAR2, acting as an epigenetic regulator, protects against colon carcinogenesis. To mimic the mild inflammation that promotes human colon cancer, we treated mice with dextran sodium sulfate (DSS) overnight, which avoids excessive inflammation but induces mild inflammation that promotes colon carcinogenesis in the ApcMin/+ and the azoxymethane (AOM)-treated mice...
March 9, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29518546/analysis-of-the-mirna-mrna-lncrna-network-in-human-estrogen-receptor-positive-and-estrogen-receptor-negative-breast-cancer-based-on-tcga-data
#9
Bin Xiao, Weiyun Zhang, Lidan Chen, Jianfeng Hang, Lizhi Wang, Rong Zhang, Yang Liao, Jianyun Chen, Qiang Ma, Zhaohui Sun, Linhai Li
Estrogen receptor-positive (ER+ ) and ER-negative (ER- ) subtypes of breast cancer have distinct clinical outcomes because they respond differentially to endocrine therapies. We aimed to comprehensively analyze differentially expressed microRNA (miRNAs), long non-coding RNAs (lncRNAs) and mRNAs in different ER subtypes as well as to identify prognosis-related RNAs. The expression levels of miRNAs, lncRNAs, and mRNAs between breast cancer and normal samples were compared using data from The Cancer Genome Atlas database...
March 5, 2018: Gene
https://www.readbyqxmd.com/read/29516551/application-of-multiplex-methylated-specific-pcr-with-capillary-electrophoresis-to-explore-prognostic-value-of-tsgs-hypermethylation-for-hepatocellular-carcinoma
#10
Yuan Huang, Ling Wei, Ai-Min Sun, Bo Li, Cheng-Jun Sun, Wei-Bo Liang, Qiu-Ying Liu, Xiao-Qin Yu, Jing-Yang He, Yang Qin
BACKGROUND: Hepatocellular carcinoma (HCC) is a malignant tumor that severely threatens human health. To date, early detection for HCC patients is particularly significant due to their poor survival rates even after liver resection. METHODS: Therefore, an efficient and sensitive detection method for monitoring liver cancer, multiplex methylation-specific PCR (MSP) coupled with capillary electrophoresis, is developed. RESULTS: Simulations demonstrated that the methylation status of RASSF1A, p16, SFRP1, and ELF could be detected even when DNA equaled or exceeded 12...
March 7, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29507645/overexpression-of-uhrf1-promotes-silencing-of-tumor-suppressor-genes-and-predicts-outcome-in-hepatoblastoma
#11
Alexander Beck, Franziska Trippel, Alexandra Wagner, Saskia Joppien, Max Felle, Christian Vokuhl, Thomas Schwarzmayr, Tim M Strom, Dietrich von Schweinitz, Gernot Längst, Roland Kappler
Background: Hepatoblastoma (HB) is the most common liver tumor of childhood and occurs predominantly within the first 3 years of life. In accordance to its early manifestation, HB has been described to display an extremely low mutation rate. As substitute, epigenetic modifiers seem to play an exceptional role in its tumorigenesis, which holds promise to develop targeted therapies and establish biomarkers for patient risk stratification. Results: We examined the role of a newly described protein complex consisting of three epigenetic regulators, namely E3 ubiquitin-like containing PHD and RING finger domain 1 (UHRF1), ubiquitin-specific-processing protease 7 (USP7), and DNA methyltransferase 1 (DNMT1), in HB...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29472591/early-sclerostin-expression-explains-bone-formation-inhibition-before-arthritis-onset-in-the-rat-adjuvant-induced-arthritis-model
#12
Guillaume Courbon, Raphaëlle Lamarque, Maude Gerbaix, Robin Caire, Marie-Thérèse Linossier, Norbert Laroche, Mireille Thomas, Thierry Thomas, Laurence Vico, Hubert Marotte
Periarticular bone loss in rheumatoid arthritis (RA) is considered to be mainly related to synovial inflammation. However, strong bone loss has also described at the time of arthritis onset. Recently, a paradoxical exacerbation of joint damage was described when blocking sclerostin in various arthritis models. Thus, we aimed to determine kinetics of bone loss and its mechanisms in the adjuvant induced arthritis (AIA) rat model of RA. AIA was induced (n = 35) or not (n = 35) at day 0. In addition to well-known arthritis at day 12, we showed with 3D-imaging and histomorphometry that bone microstructural alterations occurred early from day 8 post-induction, characterized by cortical porosity and trabecular bone loss...
February 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29455276/methylation-of-secreted-frizzled-related-protein-1-sfrp1-promoter-downregulates-wnt-%C3%AE-catenin-activity-in-keloids
#13
Jiaqi Liu, Huayu Zhu, Hongtao Wang, Jun Li, Fu Han, Yang Liu, Wanfu Zhang, Ting He, Na Li, Zhao Zheng, Dahai Hu
Keloid, a benign skin disorder, forms during wound healing in genetically susceptible individuals. To better control keloid and understand the molecular mechanisms, this study screened gene hypermethylations of GEO database microarray data on keloids and identified the hypermethylation of the secreted frizzled related protein-1 (SFRP1) promoter. Subsequently, hypermethylation and mRNA and protein levels were assessed in 57 cases of keloid vs. normal skin tissues. Fibroblasts from tissues were isolated for the assessment of gene regulation in vitro...
February 17, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29443079/gene-regulation-and-targeted-therapy-in-gastric-cancer-peritoneal-metastasis-radiological-findings-from-dual-energy-ct-and-pet-ct
#14
Bowen Shi, Huimin Lin, Miao Zhang, Wei Lu, Ying Qu, Huan Zhang
Gastric cancer remains fourth in cancer incidence worldwide with a five-year survival of only 20%-30%. Peritoneal metastasis is the most frequent type of metastasis that accompanies unresectable gastric cancer and is a definitive determinant of prognosis. Preventing and controlling the development of peritoneal metastasis could play a role in helping to prolong the survival of gastric cancer patients. A non-invasive and efficient imaging technique will help us to identify the invasion and metastasis process of peritoneal metastasis and to monitor the changes in tumor nodules in response to treatments...
January 22, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29391876/antagonism-between-hedgehog-and-wnt-signaling-pathways-regulates-tumorigenicity
#15
Mei Ding, Xin Wang
The crosstalk of multiple cellular signaling pathways is crucial in animal development and tissue homeostasis, and its dysregulation may result in tumor formation and metastasis. The Hedgehog (Hh) and Wnt signaling pathways are both considered to be essential regulators of cell proliferation, differentiation and oncogenesis. Recent studies have indicated that the Hh and Wnt signaling pathways are closely associated and involved in regulating embryogenesis and cellular differentiation. Hh signaling acts upstream of the Wnt signaling pathway, and negative regulates Wnt activity via secreted frizzled-related protein 1 (SFRP1), and the Wnt/β-catenin pathway downregulates Hh activity through glioma-associated oncogene homolog 3 transcriptional regulation...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29381095/in-vitro-effects-of-secreted-frizzled-related-protein-1-sfrp1-on-human-corneal-epithelial-cells
#16
Jingjing You, Luis Munoz-Erazo, Li Wen, Chris Hodge, Michele C Madigan, Gerard Sutton
PURPOSE: Limbal corneal epithelial cells (LCECs) are responsible for corneal epithelial cell regeneration. However, corneal central epithelial cells (CCECs) are also suggested to display potential for self-renewal. Additionally, a better understanding of molecules that regulate corneal epithelial cell regeneration is important for studying conditions affecting the cornea, for example, keratoconus. Given our previous findings of reduced levels of secreted frizzled-related protein 1 (SFRP1) in tears from keratoconus patients compared to controls, we investigated the effects of SFRP1 on the proliferation and survival of cultured central and limbal human corneal epithelial cells...
January 30, 2018: Current Eye Research
https://www.readbyqxmd.com/read/29374860/blood-collection-and-cell-free-dna-isolation-methods-influence-the-sensitivity-of-liquid-biopsy-analysis-for-colorectal-cancer-detection
#17
Barbara Kinga Barták, Alexandra Kalmár, Orsolya Galamb, Barnabás Wichmann, Zsófia Brigitta Nagy, Zsolt Tulassay, Magdolna Dank, Péter Igaz, Béla Molnár
During colorectal cancer (CRC) development tumor-derived cell-free DNA (cfDNA) can be released into the bloodstream. Many different cfDNA isolation methods and specific blood collection tubes preventing the release of genomic DNA and stabilizing cfDNA with preservative reagents became available. These factors may affect greatly on the further liquid biopsy analyses. Our aim was to test different blood collection tubes and cfDNA isolation methods to determine whether these factors influence the cfDNA amount and the promoter methylation of four previously described hypermethylated biomarkers...
January 27, 2018: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/29371858/bioinformatics-based-identification-of-mir-542-5p-as-a-predictive-biomarker-in-breast-cancer-therapy
#18
Qiong-Ni Zhu, Helen Renaud, Ying Guo
Background: Tamoxifen is the first-line hormone therapy for estrogen receptor alpha positive (ERα+) breast cancer. However, about 40% of patients with ERα + breast cancer who receive tamoxifen therapy eventually develop resistance resulting in a poor prognosis. The aim of this study was to mine available data sets in the Gene Expression Omnibus (GEO) database, including in vitro (cell lines) and in vivo (tissue samples), and to identify all miRNAs associated with tamoxifen resistance (TamR) in breast cancer...
2018: Hereditas
https://www.readbyqxmd.com/read/29358129/mir-1301-3p-promotes-prostate-cancer-stem-cell-expansion-by-targeting-sfrp1-and-gsk3%C3%AE
#19
Xian-Lu Song, Bin Huang, Bo-Wei Zhou, Chong Wang, Zhi-Wei Liao, Yi Yu, Shan-Chao Zhao
Cancer stem cells promote tumor progression, drug-resistance, and relapse, and many microRNAs (miRNAs) play critical roles in the expansion of cancer stem cells. In the present study, we investigated the role of miR-1301-3p in the expansion of prostate cancer stem cells; miR-1301-3p was significantly upregulated in prostate cancer cells and tissues compared with normal prostate cells and tissues. Sphere formation and side population assays suggested that miR-1301-3p promoted the expansion of prostate cancer stem cells, and increased the expression of prostate cancer stem cell-associated genes, such as OCT4, SOX2, NANOG, CD44, KLF4, c-MYC, and MMP2...
January 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29319176/mir-542-3p-prevents-ovariectomy-induced-osteoporosis-in-rats-via-targeting-sfrp1
#20
Xiguang Zhang, Yun Zhu, Chuanlin Zhang, Jianping Liu, Tianming Sun, Dan Li, Qiang Na, Zhaowei Teng
Secreted frizzled-related protein-1 (SFRP1) is a negative regulatory molecule of the WNT signaling pathway and serves as a therapeutic target for bone formation in osteoporosis. In this study, we first established an ovariectomized (OVX) rat model to simulate postmenopausal osteoporosis and found significant changes in miR-542-3p and sFRP1 expression by RNA sequencing and qRT-PCR. In addition, there was a significant negative correlation between miR-542-3p and sFRP1 mRNA levels in postmenopausal women with osteoporosis...
January 10, 2018: Journal of Cellular Physiology
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