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https://www.readbyqxmd.com/read/28435069/age-related-increase-in-wnt-inhibitor-causes-a-senescence-like-phenotype-in-human-cardiac-stem-cells
#1
Tamami Nakamura, Tohru Hosoyamaa, Junichi Murakami, Makoto Samura, Koji Ueno, Hiroshi Kurazumi, Ryo Suzuki, Akihito Mikamo, Kimikazu Hamano
Aging of cardiac stem/progenitor cells (CSCs) impairs heart regeneration and leads to unsatisfactory outcomes of cell-based therapies. As the precise mechanisms underlying CSC aging remain unclear, the use of therapeutic strategies for elderly patients with heart failure is severely delayed. In this study, we used human cardiosphere-derived cells (CDCs), a subtype of CSC found in the postnatal heart, to identify secreted factor(s) associated with CSC aging. Human CDCs were isolated from heart failure patients of various ages (2-83 years old)...
April 20, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28425477/microrna-27a-3p-modulates-the-wnt-%C3%AE-catenin-signaling-pathway-to-promote-epithelial-mesenchymal-transition-in-oral-squamous-carcinoma-stem-cells-by-targeting-sfrp1
#2
Bin Qiao, Bao-Xia He, Jing-Hua Cai, Qian Tao, Alfred King-Yin Lam
This study aimed to elucidate how microRNA27a-3p (miR-27a-3p) modulates the Wnt/β-catenin signaling pathway to promote the epithelial-mesenchymal transition (EMT) in oral squamous carcinoma stem cells (OSCSCs) by targeting secreted frizzled-related protein 1 (SFRP1). Flow cytometry was used to sort OSCSCs from the SCC-9 and Tca8113 cell lines. The OSCSCs were randomly assigned into the miR-27a-3p inhibitors group, the miR-27a-3p inhibitors-NC group, the si-SFRP1 group, the si-SFRP1 + miR-27a-3p inhibitors group and the blank group...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28423523/mir-106b-5p-promotes-renal-cell-carcinoma-aggressiveness-and-stem-cell-like-phenotype-by-activating-wnt-%C3%AE-catenin-signalling
#3
Jun Lu, Jin-Huan Wei, Zi-Hao Feng, Zhen-Hua Chen, Yong-Qian Wang, Yong Huang, Yong Fang, Yan-Ping Liang, Jun-Jie Cen, Yi-Hui Pan, Bing Liao, Wen-Fang Chen, Wei Chen, Jun-Hang Luo
PURPOSE: To examine the role of miR-106b-5p in regulating the cancer stem-cell-like phenotype in clear cell renal cell carcinomas (ccRCC). EXPERIMENTAL DESIGN: Real-time PCR was performed to evaluate miR-106b-5p levels in ccRCC cell lines and patients specimens. A series of in vivo and in vitro assays were performed to confirm the effect of miR-106b-5p on ccRCC stemness phenotype. RESULTS: ccRCC cells and tissues expressed more miR-106b-5p than normal controls...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28422739/diagnostic-role-of-wnt-pathway-gene-promoter-methylation-in-non-small-cell-lung-cancer
#4
Shunlin Liu, Xiaoying Chen, Ruhua Chen, Jinzhi Wang, Guoliang Zhu, Jianzhong Jiang, Hongwei Wang, Shiwei Duan, Jianan Huang
Wnt signal pathway genes are known to be involved with cancer development. Here we tested the hypothesis whether DNA methylation of genes part of the Wnt signaling pathway could help the diagnosis of non-small cell lung cancer (NSCLC). The methylation levels of SFRP1, SFRP2, WIF1 and PRKCB in 111 NSCLC patients were evaluated by quantitative methylation-specific PCR (qMSP). Promoter methylation levels of four candidate genes were significantly higher in tumor tissues compared with the adjacent tissues. SFRP1, SFRP2 and PRKCB genes were all shown to be good predictors of NSCLC risk (SFRP1: AUC = 0...
March 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28421310/comparison-of-selected-protein-levels-in-tumour-and-surgical-margin-in-a-group-of-patients-with-oral-cavity-cancer
#5
Joanna Katarzyna Strzelczyk, Karolina Gołąbek, Piotr Cuber, Łukasz Krakowczyk, Aleksander Jerzy Owczarek, Martyna Fronczek, Piotr Choręża, Edyta Hudziec, Zofia Ostrowska
Oral cavity cancer belongs to head-and-neck squamous cell carcinoma group. The purpose of the study was to assess the levels of certain proteins in a tumour and surgical margin in a group of patients with oral cavity cancer. The levels of DAPK1, MGMT, CDH1, SFRP1, SFRP2, RORA, TIMP3, p16, APC and RASSF1 proteins were measured by ELISA in tissue homogenates. The protein levels of DAPK1, MGMT, CDH1, SFRP2 and RASSF1 were significantly higher in tumour tissue than in the margin, contrary to TIMP3 which was lower in the tumour itself...
April 18, 2017: Biochemical Genetics
https://www.readbyqxmd.com/read/28418908/plasma-protein-profiling-in-patients-undergoing-coronary-artery-bypass-grafting-surgery-and-clinical-significance
#6
Zhi-Peng Guo, Hai-Tao Hou, Rui Jing, Zhen-Guo Song, Xiao-Cheng Liu, Guo-Wei He
This study was designed to identify the protein profiling in patients with triple vessel coronary artery disease (CAD) undergoing CABG, in order to detect CAD-related differential proteins in these patients. CABG patients with triple vessel disease with/without left main stenosis (n =160) were compared to normal coronary angiographic subjects (n =160). Plasma samples of 20 males and 20 females in each group were analyzed with iTRAQ technique. ELISA test was used to test the chosen proteins from iTRAQ results in plasma samples from a new cohort of the CABG group (n=120, male/femal=61/59) and control (n =120, male/female=60/60)...
March 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28402277/the-influence-of-tumor-necrosis-factor-%C3%AE-on-the-tumorigenic-wnt-signaling-pathway-in-human-mammary-tissue-from-obese-women
#7
Agathe Roubert, Kelly Gregory, Yuyang Li, Anna C Pfalzer, Jinchao Li, Sallie S Schneider, Richard J Wood, Zhenhua Liu
Epidemiological studies have convincingly suggested that obesity is an important risk factor for postmenopausal breast cancer, but the mechanisms responsible for this relationship are still not fully understood. We hypothesize that obesity creates a low-grade inflammatory microenvironment, which stimulates Wnt-signaling and thereby promotes the development of breast cancer. To test this hypothesis, we evaluated the correlations between expression of multiple inflammatory cytokines and Wnt pathway downstream genes in mammary tissues from women (age ≥ 50) undergoing reduction mammoplasty...
March 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28401000/mirna-27a-promotes-the-proliferation-and-invasion-of-human-gastric-cancer-mgc803-cells-by-targeting-sfrp1-via-wnt-%C3%AE-catenin-signaling-pathway
#8
Fang Wu, Jun Li, Ni Guo, Xiao-Hui Wang, Yu-Qian Liao
This study aims to elucidate the effects of microRNA-27a (miR-27a) on the proliferation and invasion of gastric cancer (GC) cells by targeting SFRP1 via Wnt/β-catenin signaling pathway. GC and normal adjacent tissues were collected from 273 GC patients. Human gastric cancer cell line (MGC803) and normal human gastric mucosal cell line (GES-1) were cultured. The miR-27a mRNA expression was analyzed using Quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemistry (IHC) test was used to detect miR-27a and SFRP1 protein expressions...
2017: American Journal of Cancer Research
https://www.readbyqxmd.com/read/28398872/hydrogen-peroxide-induced-secreted-frizzled-related-protein-1-gene-demethylation-contributes-to-hydrogen-peroxide-induced-apoptosis-in-human-u251-glioma-cells
#9
Zhiguo Xing, Yaping Ni, Junjie Zhao, Xudong Ma
Glioblastoma multiforme is a type of central nervous system tumor with extremely poor prognosis. Previously, hydrogen peroxide (H2O2), which promotes the oxidative stress response, has been reported to induce the apoptosis of glioma cells. Recently, secreted frizzled-related protein 1 (SFRP1) has been shown to be associated with various types of malignant tumors and with H2O2-induced oxidative stress in cardiomyocytes by negatively regulating the Wnt signaling pathway. This study aimed to explore SFRP1 expression and its roles in H2O2-induced apoptosis in human glioma cells...
February 28, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28292423/a-wnt5-activity-asymmetry-and-intercellular-signaling-via-pcp-proteins-polarize-node-cells-for-left-right-symmetry-breaking
#10
Katsura Minegishi, Masakazu Hashimoto, Rieko Ajima, Katsuyoshi Takaoka, Kyosuke Shinohara, Yayoi Ikawa, Hiromi Nishimura, Andrew P McMahon, Karl Willert, Yasushi Okada, Hiroshi Sasaki, Dongbo Shi, Toshihiko Fujimori, Toshihisa Ohtsuka, Yasunobu Igarashi, Terry P Yamaguchi, Akihiko Shimono, Hidetaka Shiratori, Hiroshi Hamada
Polarization of node cells along the anterior-posterior axis of mouse embryos is responsible for left-right symmetry breaking. How node cells become polarized has remained unknown, however. Wnt5a and Wnt5b are expressed posteriorly relative to the node, whereas genes for Sfrp inhibitors of Wnt signaling are expressed anteriorly. Here we show that polarization of node cells is impaired in Wnt5a(-/-)Wnt5b(-/-) and Sfrp mutant embryos, and also in the presence of a uniform distribution of Wnt5a or Sfrp1, suggesting that Wnt5 and Sfrp proteins act as instructive signals in this process...
March 13, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28268231/mir-1-3p-suppresses-the-proliferation-invasion-and-migration-of-bladder-cancer-cells-by-up-regulating-sfrp1-expression
#11
Anquan Shang, Man Yang, Fujun Shen, Jun Wang, Jun Wei, Weiwei Wang, Wenying Lu, Chunbin Wang, Chunlei Wang
BACKGROUND: Bladder cancer is of compelling morbidity and mortality due to its high recurrence rate. Little development has been made in the last decades in the therapy methods. Thus, the mechanism of its growth and invasiveness involving novel molecular targets are needed. OBJECTIVE: Our research objective is to confirm the hypothesis that miR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells. METHODS: The expression levels of miR-1-3p and SFRP1 were evaluated using RT-qPCR in bladder cancer tissues and cells as well as in normal tissues and cells...
March 6, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28260111/molecular-characterization-of-metastatic-osteosarcoma-differentially-expressed-genes-transcription-factors-and-micrornas
#12
Lisong Heng, Zhen Jia, Jie Bai, Kun Zhang, Yangjun Zhu, Jianbing Ma, Jun Zhang, Honghao Duan
The present study aimed to understand the molecular mechanisms underlying osteosarcoma metastasis. Microarray dataset GSE49003 was downloaded from the Gene Expression Omnibus database and used for analysis. Raw expression data were preprocessed using the preprocessCore, impute and aggregate packages in R. Differentially expressed genes (DEGs) between metastatic and non‑metastatic osteosarcoma cell lines were screened using the limma package following exclusion of DEGs with a higher significance in intra‑groups compared with inter‑groups using the genefilter package...
March 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28249195/inhibition-of-bone-formation-in-rats-by-aluminum-exposure-via-wnt-%C3%AE-catenin-pathway
#13
Xudong Sun, Haoran Wang, Wanyue Huang, Hongyan Yu, Tongtong Shen, Miao Song, Yanfei Han, Yanfei Li, Yanzhu Zhu
The previous research found that aluminum trichloride (AlCl3) inhibited rat osteoblastic differentiation through inactivation of Wnt/β-catenin signaling pathway in vitro. On that basis, the experiment in vivo was conducted in this study. Rats were orally exposed to 0 (control group) and 0.4 g/L AlCl3 (AlCl3-treated group) for 30, 60, 90 or 120 days, respectively. We found that mRNA expressions of type I collagen and insulin-like growth factor-1, mRNA and protein expressions of Runx2 and survivin, ratio of p-GSK3β/GSK3β and protein expression of β-catenin were all decreased, whereas the mRNA and protein expressions Dkk1 and sFRP1 and the mRNA expressions and activity of Caspase-3 were increased in the AlCl3-treated group compared with the control group with time prolonged...
February 20, 2017: Chemosphere
https://www.readbyqxmd.com/read/28230864/dicer-promotes-tumorigenesis-by-translocating-to-nucleus-to-promote-sfrp1-promoter-methylation-in-cholangiocarcinoma-cells
#14
Wenlong Cheng, Yongqiang Qi, Li Tian, Bing Wang, Wenhua Huang, Yongjun Chen
Dicer, a member of the RNase III family of endoribonucleases, has an important role in regulating methylation of CpG islands in mammal cancer cells. However, the underlying mechanism of action remains unclear. In this study, we demonstrated that upregulation of Dicer in cholangiocarcinoma (CCA) cells and its translocation to nuclues to interact with heterochromatin protein 1α (HP1α). The nuclear Dicer/HP1α complex appeared to promote both H3K9 trimethylation and DNA methylation of the secreted frizzled-related protein 1 (SFRP1) promoter...
February 23, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28218291/a-pan-cancer-analysis-of-secreted-frizzled-related-proteins-re-examining-their-proposed-tumour-suppressive-function
#15
Krista Marie Vincent, Lynne-Marie Postovit
Secreted frizzled-related proteins (SFRPs), containing five family members (SFRPs 1-5) are putative extracellular Wnt inhibitors. Given their abilities to inhibit Wnt signalling, as well as the loss of SFRP1 in many cancers, this family is generally considered to be tumour suppressive. In this study we analyzed gene expression, promoter methylation and survival data from over 8000 tumour and normal samples from 29 cancers in order to map the context-specific associations of SFRPs 1-5 with patient survival, gene silencing and gene expression signatures...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28178355/effects-of-exercise-training-on-circulating-levels-of-dickkpof-1-and-secreted-frizzled-related-protein-1-in-breast-cancer-survivors-a-pilot-single-blind-randomized-controlled-trial
#16
Tae Ho Kim, Jae Seung Chang, Kyu-Sang Park, Jeeyeon Park, Nahyun Kim, Jong In Lee, In Deok Kong
BACKGROUND: Wingless and integration site growth factor (Wnt) signaling is a tumorigenesis-related signaling pathway. Dickkpof-1 (DKK1) and secreted frizzled-related protein-1 (SFRP1) are endogenous negative regulators of Wnt/β-catenin signaling. Accumulating evidence indicates that higher serum levels of DKK1 are correlated with poor prognosis of various types of cancer. Here, we investigated whether exercise training causes changes in the serum levels of DKK1 and SFRP1 in patients with breast cancer...
2017: PloS One
https://www.readbyqxmd.com/read/28169332/secreted-frizzled-related-protein-1-overexpression-in-gastric-cancer-relationship-with-radiological-findings-of-dual-energy-spectral-ct-and-pet-ct
#17
Huimin Lin, Guoyuan Yang, Bei Ding, Miao Zhang, Mingjun Zhang, Fuhua Yan, Ying Qu, Huan Zhang
We explored the role of secreted frizzled-related protein 1 (sFRP1) overexpression in gastric cancer and its relationship with radiological findings from dual-energy spectral CT(DEsCT) and positron emission tomography/computed tomography (PET/CT). We established mouse metastatic models using the SGC-7901/sFRP1 gastric cancer cell line. A control group was established using the SGC-7901/vector cell line. The models were then scanned with dual-energy spectral CT and PET-CT. Subsequent analysis, including immunohistochemistry and Transferase-mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL), was performed to confirm the role of sFRP1...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28148542/predicting-the-grade-of-dysplasia-of-pancreatic-cystic-neoplasms-using-cyst-fluid-dna-methylation-markers
#18
Tatsuo Hata, Marco Dal Molin, Seung-Mo Hong, Koji Tamura, Masaya Suenaga, Jun Yu, Hiraku Sedogawa, Matthew J Weiss, Christopher L Wolfgang, Anne Marie Lennon, Ralph H Hruban, Michael G Goggins
Purpose: Pancreatic cysts are common and pose diagnostic and management challenges. Pancreatic cyst fluid markers have the potential to aid in the management of cysts with concerning imaging findings. Our aim was to evaluate cyst fluid methylated DNA markers for their accuracy for predicting the histologic grade of neoplastic pancreatic cysts.Experimental Design: Pancreatic cyst fluid samples from 183 patients (29 discovery and 154 validation) aspirated after surgical resection were analyzed for methylated DNA at selected genes (SOX17, BNIP3, FOXE1, PTCHD2, SLIT2, EYA4, and SFRP1) using methylation-specific droplet-digital PCR (dd-QMSP)...
February 1, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28107193/microrna-744-promotes-prostate-cancer-progression-through-aberrantly-activating-wnt-%C3%AE-catenin-signaling
#19
Han Guan, Chunhui Liu, Fang Fang, Yeqing Huang, Tao Tao, Zhixin Ling, Zonghao You, Xu Han, Shuqiu Chen, Bin Xu, Ming Chen
Accumulated evidence indicate that miR-744 functions as either tumor suppressor or oncogene in the progression of a variety of tumors, with a tumor type-specific way. However, little is known about how miR-744 impacts on the tumorigenesis of human prostate cancer. In this study, employing the analyses of microarray, qRT-PCR and re-analysis of MSKCC data, we found that CRPC tissues expressed much more miR-744 than ADPC tissues did, and the expression level of miR-744 was inversely associated with survival of CRPC patients...
February 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28105693/epigenetic-regulation-of-wnt-%C3%AE-catenin-signal-associated-genes-in-gastric-neoplasia-of-the-fundic-gland-chief-cell-predominant-type
#20
Takashi Murakami, Hiroyuki Mitomi, Takashi Yao, Tsuyoshi Saito, Tomoyoshi Shibuya, Sumio Watanabe
Gastric neoplasia of the fundic gland (chief cell-predominant) type (GNCCP) is a rare variant of gastric tumor. This tumor is associated with activation of the Wnt/β-catenin signaling pathway; however, the mechanisms underlying this activation remain unknown. To elucidate potential roles of Wnt/β-catenin signal-associated gene methylation in GNCCP, we performed β-catenin immunostaining and methylation-specific polymerase chain reaction (PCR) for their associated genes, including SFRPs, APC, AXIN2, and MCC, in 26 GNCCPs [i...
March 2017: Pathology International
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