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https://www.readbyqxmd.com/read/28225061/dictionary-enhanced-imaging-cytometry
#1
Antony Orth, Diane Schaak, Ethan Schonbrun
State-of-the-art high-throughput microscopes are now capable of recording image data at a phenomenal rate, imaging entire microscope slides in minutes. In this paper we investigate how a large image set can be used to perform automated cell classification and denoising. To this end, we acquire an image library consisting of over one quarter-million white blood cell (WBC) nuclei together with CD15/CD16 protein expression for each cell. We show that the WBC nucleus images alone can be used to replicate CD expression-based gating, even in the presence of significant imaging noise...
February 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28196867/synaptic-activity-drives-a-genomic-program-that-promotes-a-neuronal-warburg-effect
#2
Carlos Bas-Orth, Yan-Wei Tan, David Lau, Hilmar Bading
Synaptic activity drives changes in gene expression to promote long-lasting adaptations of neuronal structure and function. One example of such an adaptive response is the buildup of acquired neuroprotection, a synaptic activity- and gene transcription-mediated increase in the resistance of neurons against harmful conditions. A hallmark of acquired neuroprotection is the stabilization of mitochondrial structure and function. We therefore re-examined previously identified sets of synaptic activity-regulated genes to identify genes that are directly linked to mitochondrial function...
February 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28196044/is-oxinium-losing-its-luster-commentary-on-an-article-by-christopher-j-vertullo-mbbs-fracs-orth-faortha-et-al-twelve-year-outcomes-of-an-oxinium-total-knee-replacement-compared-with-the-same-cobalt-chromium-design-an-analysis-of-17-577-prostheses-from-the-australian
#3
https://www.readbyqxmd.com/read/28194306/a-randomized-exploratory-phase-2-study-in-patients-with-chemotherapy-related-peripheral-neuropathy-evaluating-whole-body-vibration-training-as-adjunct-to-an-integrated-program-including-massage-passive-mobilization-and-physical-exercises
#4
Stefan S Schönsteiner, Heidi Bauder Mißbach, Axel Benner, Silja Mack, Thomas Hamel, Michael Orth, Bernhard Landwehrmeyer, Sigurd D Süßmuth, Carolin Geitner, Regine Mayer-Steinacker, Anneliese Riester, Andrea Prokein, Elfriede Erhardt, Jelena Kunecki, Anna M Eisenschink, Rainer Rawer, Hartmut Döhner, Elisabeth Kirchner, Richard F Schlenk
BACKGROUND: Chemotherapy-induced polyneuropathy (CIPN) is a common toxicity after chemotherapy, immunomodulatory drugs or proteasome inhibitors, which is difficult to treat and may also have impact on quality of life. The objective of the study was to evaluate whole-body vibration (WBV) on the background of an integrated program (IP) including massage, passive mobilization and physical exercises on CIPN. PATIENTS AND METHODS: In an exploratory phase-2 study patients with CIPN (NCI CTC grade 2/3) were randomized for WBV plus IP (experimental) to IP alone (standard)...
2017: Experimental Hematology & Oncology
https://www.readbyqxmd.com/read/28182449/the-family-environment-in-early-childhood-has-a-long-term-effect-on-self-esteem-a-longitudinal-study-from-birth-to-age-27-years
#5
Ulrich Orth
A better understanding is needed of the factors that shape the development of individual differences in self-esteem. Using a prospective longitudinal design, this research tested whether the family environment in early childhood predicts self-esteem in later developmental periods. Data came from a nationally representative U.S. sample of 8,711 participants, who reported on their self-esteem biannually from age 8 to 27 years. Moreover, during the participants' first 6 years of life, biannual assessments of their mothers provided information on the quality of the home environment (covering quality of parenting, cognitive stimulation, and physical home environment), quality of parental relationship, presence of father, maternal depression, and poverty status of the family...
February 9, 2017: Journal of Personality and Social Psychology
https://www.readbyqxmd.com/read/28176612/comparison-of-the-sysmex-xt-2000iv-with-microscopic-differential-counts-of-canine-bone-marrow
#6
Iris Pernecker, Natali B Bauer, Sigrid Johannes, Melanie Ginder, Johannes H Harleman, Andreas Moritz
Canine bone marrow is frequently assessed in the advanced preclinical research environment. Automated analysis provides time savings and objectivity over the gold standard of microscopic (cytologic) evaluation. We compared the analysis of 90 canine bone marrow samples by the Sysmex XT-2000iV hematology analyzer (Sysmex Corp., Kobe, Japan) with cytologic evaluation. Gates for cell populations were created in the system's WBC/BASO channel. Variables "total nucleated red blood cells" (total_NRBC), "poly- and orthochromatic nucleated red blood cells" (poly_orth_NRBC), "total neutrophils" (total_NEUT), "mature neutrophils" (mature_NEUT), and myeloid-to-erythroid (M:E) ratio were compared with cytologic evaluation...
January 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28170175/genetic-modification-of-human-peripheral-blood-aspirates-using-recombinant-adeno-associated-viral-vectors-for-articular-cartilage-repair-with-a-focus-on-chondrogenic-transforming-growth-factor-%C3%AE-gene-delivery
#7
Janina Frisch, Patrick Orth, Jagadeesh Kumar Venkatesan, Ana Rey-Rico, Gertrud Schmitt, Dieter Kohn, Henning Madry, Magali Cucchiarini
Transplantation of genetically modified peripheral blood aspirates that carry chondrogenically competent progenitor cells may offer new, convenient tools to treat articular cartilage lesions compared with the more complex and invasive application of bone marrow concentrates or of bone marrow-derived mesenchymal stem cells. Here, we show that recombinant adeno-associated viral (rAAV) vectors are powerful gene vehicles capable of successfully targeting primary human peripheral blood aspirates in a stable and safe manner, allowing for an efficient and long-term transgene expression in such samples (up to 63 days with use of a lacZ reporter gene and for at least 21 days with application of the pleiotropic, chondrogenic factor transforming growth factor-β [TGF-β])...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28168210/white-matter-predicts-functional-connectivity-in-premanifest-huntington-s-disease
#8
Peter McColgan, Sarah Gregory, Adeel Razi, Kiran K Seunarine, Fatma Gargouri, Alexandra Durr, Raymund A C Roos, Blair R Leavitt, Rachael I Scahill, Chris A Clark, Sarah J Tabrizi, Geraint Rees, A Coleman, J Decolongon, M Fan, T Petkau, C Jauffret, D Justo, S Lehericy, K Nigaud, R Valabrègue, A Choonderbeek, E P T Hart, D J Hensman Moss, H Crawford, E Johnson, M Papoutsi, C Berna, R Reilmann, N Weber, J Stout, I Labuschagne, B Landwehrmeyer, M Orth, H Johnson
OBJECTIVES: The distribution of pathology in neurodegenerative disease can be predicted by the organizational characteristics of white matter in healthy brains. However, we have very little evidence for the impact these pathological changes have on brain function. Understanding any such link between structure and function is critical for understanding how underlying brain pathology influences the progressive behavioral changes associated with neurodegeneration. Here, we demonstrate such a link between structure and function in individuals with premanifest Huntington's...
February 2017: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/28165203/multiple-stressors-threaten-the-imperiled-coastal-foundation-species-eelgrass-zostera-marina-in-chesapeake-bay-usa
#9
Jonathan S Lefcheck, David J Wilcox, Rebecca R Murphy, Scott R Marion, Robert J Orth
Interactions among global change stressors and their effects at large scales are often proposed, but seldom evaluated. This situation is primarily due to lack of comprehensive, sufficiently long-term, and spatially extensive datasets. Seagrasses, which provide nursery habitat, improve water quality, and constitute a globally important carbon sink, are among the most vulnerable habitats on the planet. Here, we unite 31 years of high-resolution aerial monitoring and water quality data to elucidate the patterns and drivers of eelgrass (Zostera marina) abundance in Chesapeake Bay, USA, one of the largest and most valuable estuaries in the world, with an unparalleled history of regulatory efforts...
February 6, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28149323/the-european-network-on-psychosomatic-medicine-enpm-history-and-future-directions
#10
REVIEW
Hans-Christian Deter, Kristina Orth-Gomér, Bohdan Wasilewski, Ramiro Verissimo
BACKGROUND: Within national and international societies of psychosomatic medicine the idea has emerged of bringing together and coordinating psychosomatic, behavioural, psychological and medical actions with common interests throughout Europe as a way to increase their scientific and political influence. METHODS: It was felt that there was a strong need and opportunity of a common and unifying forum for scientific exchange. RESULTS: It was considered desirable to exchange scientific thoughts and experiences in an open minded and boundless way, among individuals and societies, between disciplines and across borders...
2017: BioPsychoSocial Medicine
https://www.readbyqxmd.com/read/28139287/depletion-rate-of-hydrogen-peroxide-from-sodium-perborate-bleaching-agent
#11
Liliann Tran, Rebecca Orth, Peter Parashos, Ying Tao, Calvin W J Tee, Vineet Thenalil Thomas, Georgina Towers, Diem Thuy Truong, Cynthia Vinen, Eric C Reynolds
INTRODUCTION: Internal bleaching of discolored teeth uses sodium perborate reacting with water to form the active agent, hydrogen peroxide (H2O2). Sodium perborate is replaced at varying time intervals depending on clinician preference and until esthetically acceptable results are achieved, but this is done without scientific basis. This study measured the depletion rate of hydrogen peroxide from sodium perborate as a bleaching agent. METHODS: Two sodium perborate bleaching products (Odontobleach [Australian Dental Manufacturing, Kenmore Hills, Queensland, Australia] and Endosure Perborate Micro [Dentalife, Ringwood, Victoria, Australia]) and distilled deionized water mixtures at ratios of 25 μg/mL, 50 μg/mL, and 100 μg/mL were placed into sealed microtubes and incubated at 37°C...
January 27, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28129014/biochemical-basis-for-activation-of-virulence-genes-by-bile-salts-in-vibrio-parahaemolyticus
#12
Giomar Rivera-Cancel, Kim Orth
Bile salts act as a stressor to bacteria that transit the intestinal tract. Enteric pathogens have hijacked bile as an intestinal signal to regulate virulence factors. We recently demonstrated that Vibrio parahemolyticus senses bile salts via a heterodimeric receptor formed by the periplasmic domains of inner-membrane proteins VtrA and VtrC. Crystal structures of the periplasmic complex reveal that VtrA and VtrC form a β-barrel that binds bile salts in its hydrophobic interior to activate the VtrA cytoplasmic DNA-binding domain...
January 27, 2017: Gut Microbes
https://www.readbyqxmd.com/read/28098782/auranofin-inhibits-the-enzyme-activity-of-pasteurella-multocida-toxin-pmt-in-human-cells-and-protects-cells-from-intoxication
#13
Stefan Carle, Thorsten Brink, Joachim H C Orth, Klaus Aktories, Holger Barth
The AB-type protein toxin from Pasteurella multocida (PMT) contains a functionally important disulfide bond within its catalytic domain, which must be cleaved in the host cell cytosol to render the catalytic domain of PMT into its active conformation. Here, we found that the reductive potential of the cytosol of target cells, and more specifically, the activity of the thioredoxin reductase (TrxR) is crucial for this process. This was demonstrated by the strong inhibitory effect of the pharmacological TrxR inhibitor auranofin, which inhibited the intoxication of target cells with PMT, as determined by analyzing the PMT-catalyzed deamidation of GTP-binding proteins (G-proteins) in the cytosol of cells...
January 13, 2017: Toxins
https://www.readbyqxmd.com/read/28068668/the-hippo-kinases-lats1-and-2-control-human-breast-cell-fate-via-crosstalk-with-er%C3%AE
#14
Adrian Britschgi, Stephan Duss, Sungeun Kim, Joana Pinto Couto, Heike Brinkhaus, Shany Koren, Duvini De Silva, Kirsten D Mertz, Daniela Kaup, Zsuzsanna Varga, Hans Voshol, Alexandra Vissieres, Cedric Leroy, Tim Roloff, Michael B Stadler, Christina H Scheel, Loren J Miraglia, Anthony P Orth, Ghislain M C Bonamy, Venkateshwar A Reddy, Mohamed Bentires-Alj
Cell fate perturbations underlie many human diseases, including breast cancer. Unfortunately, the mechanisms by which breast cell fate are regulated are largely unknown. The mammary gland epithelium consists of differentiated luminal epithelial and basal myoepithelial cells, as well as undifferentiated stem cells and more restricted progenitors. Breast cancer originates from this epithelium, but the molecular mechanisms that underlie breast epithelial hierarchy remain ill-defined. Here, we use a high-content confocal image-based short hairpin RNA screen to identify tumour suppressors that regulate breast cell fate in primary human breast epithelial cells...
January 26, 2017: Nature
https://www.readbyqxmd.com/read/28054333/bmp-2-coated-mineral-coated-microparticles-improve-bone-repair-in-atrophic-non-unions
#15
M Orth, N J Kruse, B J Braun, C Scheuer, J H Holstein, A Khalil, X Yu, W L Murphy, T Pohlemann, M W Laschke, M D Menger
Atrophic non-unions are a major clinical problem. Mineral coated microparticles (MCM) are electrolyte-coated hydroxyapatite particles that have been shown in vitro to bind growth factors electrostatically and enable a tuneable sustained release. Herein, we studied whether MCM can be used in vivo to apply Bone Morphogenetic Protein-2 (BMP-2) to improve bone repair of atrophic non-unions. For this purpose, atrophic non-unions were induced in femurs of CD-1 mice (n = 48). Animals either received BMP-2-coated MCM (MCM + BMP; n = 16), uncoated MCM (MCM; n = 16) or no MCM (NONE; n = 16)...
January 2, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/27996032/targeting-bacterial-adherence-inhibits-multidrug-resistant-pseudomonas-aeruginosa-infection-following-burn-injury
#16
Ryan M Huebinger, Daniel H Stones, Marcela de Souza Santos, Deborah L Carlson, Juquan Song, Diana Pereira Vaz, Emma Keen, Steven E Wolf, Kim Orth, Anne Marie Krachler
Classical antimicrobial drugs target proliferation and therefore place microbes under extreme selective pressure to evolve resistance. Alternative drugs that target bacterial virulence without impacting survival directly offer an attractive solution to this problem, but to date few such molecules have been discovered. We previously discovered a widespread group of bacterial adhesins, termed Multivalent Adhesion Molecules (MAMs) that are essential for initial binding of bacteria to host tissues and virulence...
December 20, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27983563/important-but-not-enough-information-about-hd-related-topics-and-peer-and%C3%A2-professional-support-for-young-adults-from-hd-families
#17
Ulrike Braisch, Saul Martinez-Horta, Marcy MacDonald, Michael Orth
BACKGROUND: The number of people affected by Huntington's disease (HD) is far greater than those with manifest HD because it also includes those at risk, both HD gene mutation carriers and family members not carrying the HD mutation. Many relevant needs of young adults from HD families may not be met at present. This includes advice on important life decisions e.g. family planning and having children, psychological support and treatment of medical conditions. OBJECTIVE: To survey the opinion of young adults from HD families about relevance and availability of information and support regarding several aspects of HD...
December 15, 2016: Journal of Huntington's Disease
https://www.readbyqxmd.com/read/27933948/discovery-of-the-3-imino-1-2-4-thiadiazinane-1-1-dioxide-derivative-verubecestat-mk-8931-a-%C3%AE-site-amyloid-precursor-protein-cleaving-enzyme-1-inhibitor-for-the-treatment-of-alzheimer-s-disease
#18
Jack D Scott, Sarah W Li, Andrew P J Brunskill, Xia Chen, Kathleen Cox, Jared N Cumming, Mark Forman, Eric J Gilbert, Robert A Hodgson, Lynn A Hyde, Qin Jiang, Ulrich Iserloh, Irina Kazakevich, Reshma Kuvelkar, Hong Mei, John Meredith, Jeffrey Misiaszek, Peter Orth, Lana M Rossiter, Meagan Slater, Julie Stone, Corey O Strickland, Johannes H Voigt, Ganfeng Wang, Hongwu Wang, Yusheng Wu, William J Greenlee, Eric M Parker, Matthew E Kennedy, Andrew W Stamford
Verubecestat 3 (MK-8931), a diaryl amide-substituted 3-imino-1,2,4-thiadiazinane 1,1-dioxide derivative, is a high-affinity β-site amyloid precursor protein cleaving enzyme 1 (BACE1) inhibitor currently undergoing Phase 3 clinical evaluation for the treatment of mild to moderate and prodromal Alzheimer's disease. Although not selective over the closely related aspartyl protease BACE2, verubecestat has high selectivity for BACE1 over other key aspartyl proteases, notably cathepsin D, and profoundly lowers CSF and brain Aβ levels in rats and nonhuman primates and CSF Aβ levels in humans...
December 8, 2016: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27929302/age-time-period-and-birth-cohort-differences-in-self-esteem-reexamining-a-cohort-sequential-longitudinal-study
#19
Jean M Twenge, Nathan T Carter, W Keith Campbell
Orth, Trzesniewski, and Robins (2010) concluded that the nationally representative Americans' Changing Lives (ACL) cohort-sequential study demonstrated moderate to large age differences in self-esteem, and no birth cohort (generational) differences in the age trajectory. In a reanalysis of these data using 2 different statistical techniques, we find significant increases in self-esteem that could be attributed to birth cohort or time period. First, hierarchical linear modeling analyses with birth cohort as a continuous variable (vs...
December 8, 2016: Journal of Personality and Social Psychology
https://www.readbyqxmd.com/read/27889468/mitochondrial-cristae-remodelling-is-associated-with-disrupted-opa1-oligomerisation-in-the-huntington-s-disease-r6-2-fragment-model
#20
Tanja Hering, Kerstin Kojer, Nathalie Birth, Jaqueline Hallitsch, Jan-Willem Taanman, Michael Orth
There is evidence of an imbalance of mitochondrial fission and fusion in patients with Huntington's disease (HD) and HD animal models. Fission and fusion are important for mitochondrial homeostasis including mitochondrial DNA (mtDNA) maintenance and may be relevant for the selective striatal mtDNA depletion that we observed in the R6/2 fragment HD mouse model. We aimed to investigate the fission/fusion balance and the integrity of the mitochondrial membrane system in cortex and striatum of end-stage R6/2 mice and wild-type animals...
February 2017: Experimental Neurology
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