keyword
MENU ▼
Read by QxMD icon Read
search

Vgsc

keyword
https://www.readbyqxmd.com/read/29776379/development-of-a-rapid-field-applicable-molecular-diagnostic-for-knockdown-resistance-kdr-markers-in-an-gambiae
#1
Vera T Unwin, Shaun Ainsworth, Emily J Rippon, El Hadji Amadou Niang, Mark J I Paine, David Weetman, Emily R Adams
BACKGROUND: The spread of insecticide resistance (IR) is a major threat to vector control programmes for mosquito-borne diseases. Early detection of IR using diagnostic markers could help inform these programmes, especially in remote locations where gathering reliable bioassay data is challenging. Most current molecular tests for genetic IR markers are only suitable for use in well-equipped laboratory settings. There is an unmet need for field-applicable diagnostics. METHODS: A single-cartridge test was designed to detect key IR mutations in the major African vector of malaria, Anopheles gambiae...
May 18, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29776361/emergence-of-knock-down-resistance-in-the-anopheles-gambiae-complex-in-the-upper-river-region-the-gambia-and-its-relationship-with-malaria-infection-in-children
#2
Anne L Wilson, Margaret Pinder, John Bradley, Martin J Donnelly, Majidah Hamid-Adiamoh, Lamin B S Jarju, Musa Jawara, David Jeffries, Ballah Kandeh, Emily J Rippon, Kolawole Salami, Umberto D'Alessandro, Steven W Lindsay
BACKGROUND: Insecticide resistance threatens malaria control in sub-Saharan Africa. Knockdown resistance to pyrethroids and organochlorines in Anopheles gambiae sensu lato (s.l.) is commonly caused by mutations in the gene encoding a voltage-gated sodium channel which is the target site for the insecticide. The study aimed to examine risk factors for knockdown resistance in An. gambiae s.l. and its relationship with malaria infection in children in rural Gambia. Point mutations at the Vgsc-1014 locus, were measured in An...
May 18, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29712956/parallel-evolution-of-vgsc-mutations-at-domains-is6-iis6-and-iiis6-in-pyrethroid-resistant-aedes-aegypti-from-mexico
#3
Karla Saavedra-Rodriguez, Farah Vera Maloof, Corey L Campbell, Julian Garcia-Rejon, Audrey Lenhart, Patricia Penilla, Americo Rodriguez, Arturo Acero Sandoval, Adriana E Flores, Gustavo Ponce, Saul Lozano, William C Black
Aedes aegypti is the primary urban mosquito vector of viruses causing dengue, Zika and chikungunya fevers -for which vaccines and effective pharmaceuticals are still lacking. Current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larval and adult populations. Insecticidal control of Ae. aegypti is challenging, due to a recent rapid global increase in knockdown-resistance (kdr) to pyrethroid insecticides. Widespread, heavy use of pyrethroid space-sprays has created an immense selection pressure for kdr, which is primarily under the control of the voltage-gated sodium channel gene (vgsc)...
April 30, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29673714/discovery-and-evaluation-of-nna-v-1-5-sodium-channel-blockers-with-potent-cell-invasion-inhibitory-activity-in-breast-cancer-cells
#4
Shilpa Dutta, Osbaldo Lopez Charcas, Samuel Tanner, Frédéric Gradek, Virginie Driffort, Sébastien Roger, Katri Selander, Sadanandan E Velu, Wayne Brouillette
Voltage-gated sodium channels (VGSC) are a well-established drug target for anti-epileptic, anti-arrhythmic and pain medications due to their presence and the important roles that they play in excitable cells. Recently, their presence has been recognized in non-excitable cells such as cancer cells and their overexpression has been shown to be associated with metastatic behavior in a variety of human cancers. The neonatal isoform of the VGSC subtype, Nav 1.5 (nNav 1.5) is overexpressed in the highly aggressive human breast cancer cell line, MDA-MB-231...
April 3, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29670071/susceptibility-status-and-resistance-mechanisms-in-permethrin-selected-laboratory-susceptible-and-field-collected-aedes-aegypti-from-malaysia
#5
Rosilawati Rasli, Han Lim Lee, Nazni Wasi Ahmad, Siti Futri Farahininajua Fikri, Roziah Ali, Khairul Asuad Muhamed, Azahari Abdul Hadi, Qi-Yong Liu, Feng Xia Meng
This study is intended to provide a comprehensive characterization of the resistance mechanisms in the permethrin-selected (IMR-PSS) and laboratory susceptible (IMR-LS) Aedes aegypti strain from Malaysia. Both IMR-PSS and IMR-LS provide a standard model for use in assessing the pyrethroid resistance in field-collected strains collected from three dengue hotspots: the Taman Seri Bayu (TSB), the Flat Camar (FC), and the Taman Dahlia (TD). Two established methods for determining the resistance mechanisms of the pyrethroid are the quantification of detoxification enzymes via enzyme microassay and the nucleotide sequencing of the domain 2 region from segment 1 to 6 via classical polymerase chain reaction (PCR) amplification-were employed...
April 18, 2018: Insects
https://www.readbyqxmd.com/read/29626998/first-report-of-target-site-insensitivity-to-pyrethroids-in-human-flea-pulex-irritans-siphonaptera-pulicidae
#6
Mohammad Bagher Ghavami, Fahimeh Pourrastgou Haghi, Zohreh Alibabaei, Ahmad Ali Enayati, Hassan Vatandoost
The human flea, Pulex irritans, is the most important ectoparasite of humans. Intensive use of pyrethroids for its control has led to insecticide resistance. Monitoring pyrethroid resistance and its underlying mechanisms is essential for flea control. The aims of this study were to identify the susceptibility status of human flea to permethrin and to detect the presence of knockdown resistance (kdr) mutation and its frequency in populations of P. irritans. Adults of P. irritans were collected from Zanjan Province, northwest of Iran, during 2013-2017...
April 2018: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/29562293/distribution-and-frequency-of-pyrethroid-resistance-associated-mutations-in-host-lineages-of-the-bed-bug-hemiptera-cimicidae-across-europe
#7
Ondrej Balvín, Warren Booth
For over two decades, the bed bug, Cimex lectularius L. (Hemiptera: Cimicidae) has been undergoing a dramatic global resurgence, likely in part to the evolution of mechanisms conferring resistance to insecticides. One such mechanism is knock-down resistance (kdr), resulting from nonsynonymous mutations within the voltage-gated sodium channel (VGSC) gene. To date, three mutations have been identified in C. lectularius, V419L, L925I, and I936F. Using Sanger sequencing, the frequency and distribution of these VGSC mutations across 131 populations collected from the bat-associated and human-associated lineages of C...
March 15, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29514130/effects-of-polysaccharide-from-portulaca-oleracea-l-on-voltage-gated-na-channel-of-ins-1-cells
#8
Qingjuan Hu, Yuping Li, Chao Liu, Li-Ping Huang, Lvming Zeng, Songhua Wang, Hao Song, Hailong Peng, Jianhua Huang, Chong Chen, Li-Hua Yao
Our previous work showed that polysaccharide isolated from Portulaca oleracea L. (POP) has an insulinotropic effect. The voltage-gated Na+ channel (VGSC) in the excitement phase plays an important role. This work aims to study the effect of POP on the voltage-gated Na+ channel current (INa ) and its channel dynamic characteristics in insulin-secreting β-cell line (INS-1) cells of rat. Our results revealed that POP can inhibit the amplitude of INa and improve cell survival in a concentration-dependent manner...
May 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29499751/temporal-and-spatial-trends-in-insecticide-resistance-in-anopheles-arabiensis-in-sudan-outcomes-from-an-evaluation-of-implications-of-insecticide-resistance-for-malaria-vector-control
#9
Bashir Adam Ismail, Hmooda Toto Kafy, Jihad Eltaher Sulieman, Krishanthi Subramaniam, Brent Thomas, Abraham Mnzava, Nur Faeza Abu Kassim, Abu Hassan Ahmad, Tessa B Knox, Immo Kleinschmidt, Martin J Donnelly
BACKGROUND: Long-lasting insecticidal nets (LLINs) (with pyrethroids) and indoor residual spraying (IRS) are the cornerstones of the Sudanese malaria control program. Insecticide resistance to the principal insecticides in LLINs and IRS is a major concern. This study was designed to monitor insecticide resistance in Anopheles arabiensis from 140 clusters in four malaria-endemic areas of Sudan from 2011 to 2014. All clusters received LLINs, while half (n = 70), distributed across the four regions, had additional IRS campaigns...
March 2, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29471128/spatiotemporal-magnetic-fields-enhance-cytosolic-ca-2-levels-and-induce-actin-polymerization-via-activation-of-voltage-gated-sodium-channels-in-skeletal-muscle-cells
#10
Mónica Rubio Ayala, Tatiana Syrovets, Susanne Hafner, Vitalii Zablotskii, Alexandr Dejneka, Thomas Simmet
Cellular function is modulated by the electric membrane potential controlling intracellular physiology and signal propagation from a motor neuron to a muscle fiber resulting in muscle contraction. Unlike electric fields, magnetic fields are not attenuated by biological materials and penetrate deep into the tissue. We used complex spatiotemporal magnetic fields (17-70 mT) to control intracellular signaling in skeletal muscle cells. By changing different parameters of the alternating magnetic field (amplitude, inversion time, rotation frequency), we induced transient depolarization of cellular membranes leading to i) Na+ influx through voltage-gated sodium channels (VGSC), ii) cytosolic calcium increase, and iii) VGSC- and ryanodine receptor-dependent increase of actin polymerization...
May 2018: Biomaterials
https://www.readbyqxmd.com/read/29464397/mining-protein-evolution-for-insights-into-mechanisms-of-voltage-dependent-sodium-channel-auxiliary-subunits
#11
Steven Molinarolo, Daniele Granata, Vincenzo Carnevale, Christopher A Ahern
Voltage-gated sodium channel (VGSC) beta (β) subunits have been called the "overachieving" auxiliary ion channel subunit. Indeed, these subunits regulate the trafficking of the sodium channel complex at the plasma membrane and simultaneously tune the voltage-dependent properties of the pore-forming alpha-subunit. It is now known that VGSC β-subunits are capable of similar modulation of multiple isoforms of related voltage-gated potassium channels, suggesting that their abilities extend into the broader voltage-gated channels...
February 21, 2018: Handbook of Experimental Pharmacology
https://www.readbyqxmd.com/read/29450890/efficacy-and-safety-of-eslicarbazepine-acetate-monotherapy-in-patients-converting-from-carbamazepine
#12
Ladislav Pazdera, Michael R Sperling, Jay H Harvey, Maria C Sam, Laura A Strom, David Blum, Todd Grinnell, Hailong Cheng
OBJECTIVE: To evaluate the influence of prior use of carbamazepine (CBZ) and other antiepileptic drugs (AEDs) with a putatively similar mechanism of action (inhibition of voltage-gated sodium channels; VGSCs) on seizure outcomes and tolerability when converting to eslicarbazepine acetate (ESL), using data pooled from 2 controlled conversion-to-ESL monotherapy trials (studies: 093-045, 093-046). METHODS: Adults with treatment-resistant focal (partial-onset) seizures were randomized 2:1 to ESL 1600 or 1200 mg once daily...
March 2018: Epilepsia
https://www.readbyqxmd.com/read/29440767/candidate-gene-based-gwas-identifies-reproducible-dna-markers-for-metabolic-pyrethroid-resistance-from-standing-genetic-variation-in-east-african-anopheles-gambiae
#13
David Weetman, Craig S Wilding, Daniel E Neafsey, Pie Müller, Eric Ochomo, Alison T Isaacs, Keith Steen, Emily J Rippon, John C Morgan, Henry D Mawejje, Daniel J Rigden, Loyce M Okedi, Martin J Donnelly
Metabolic resistance to pyrethroid insecticides is widespread in Anopheles mosquitoes and is a major threat to malaria control. DNA markers would aid predictive monitoring of resistance, but few mutations have been discovered outside of insecticide-targeted genes. Isofemale family pools from a wild Ugandan Anopheles gambiae population, from an area where operational pyrethroid failure is suspected, were genotyped using a candidate-gene enriched SNP array. Resistance-associated SNPs were detected in three genes from detoxification superfamilies, in addition to the insecticide target site (the Voltage Gated Sodium Channel gene, Vgsc)...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29427204/reduced-cooperativity-of-voltage-gated-sodium-channels-in-the-hippocampal-interneurons-of-an-aged-mouse-model-of-alzheimer-s-disease
#14
Carlos Perez, Ghanim Ullah
Beta amyloid (A[Formula: see text] ) associated with Alzheimer's disease (AD) leads to abnormal behavior in inhibitory neurons, resulting in hyperactive neuronal networks, epileptiform behavior, disrupted gamma rhythms, and aberrant synaptic plasticity. Previously, we used a dual modeling-experimental approach to explain several observations, including failure to reliably produce action potentials (APs), smaller AP amplitudes, higher resting membrane potential, and higher membrane depolarization in response to a range of stimuli in hippocampal inhibitory neurons from 12- to 16-month-old female AP Pswe/PSEN1DeltaE9 (APdE9) AD mice as compared to age-matched non-transgenic (NTG) mice...
February 9, 2018: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/29402835/voltage-gated-na-channels-are-modulated-by-glucose-and-involved-in-regulating-cellular-insulin-content-of-ins-1-cells
#15
Chong Chen, Songhua Wang, Qingjuan Hu, Lvming Zeng, Hailong Peng, Chao Liu, Li-Ping Huang, Hao Song, Yuping Li, Li-Hua Yao, Wei Meng
BACKGROUND/AIMS: Islet beta cells (β-cells) are unique cells that play a critical role in glucose homeostasis by secreting insulin in response to increased glucose levels. Voltage-gated ion channels in β-cells, such as K+ and Ca2+ channels, contribute to insulin secretion. The response of voltage-gated Na+ channels (VGSCs) in β-cells to the changes in glucose levels remains unknown. This work aims to determine the role of extracellular glucose on the regulation of VGSC. METHODS: The effect of glucose on VGSC currents (INa) was investigated in insulin-secreting β-cell line (INS-1) cells of rats using whole-cell patch clamp techniques, and the effects of glucose on insulin content and cell viability were determined using Enzyme-Linked Immunosorbent Assay (ELISA) and Methylthiazolyldiphenyl-tetrazolium Bromide (MTT) assay methods respectively...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29330525/distinct-modulation-of-inactivation-by-a-residue-in-the-pore-domain-of-voltage-gated-na-channels-mechanistic-insights-from-recent-crystal-structures
#16
Rene Cervenka, Peter Lukacs, Vaibhavkumar S Gawali, Song Ke, Xaver Koenig, Lena Rubi, Touran Zarrabi, Karlheinz Hilber, Walter Sandtner, Anna Stary-Weinzinger, Hannes Todt
Inactivation of voltage-gated Na+ channels (VGSC) is essential for the regulation of cellular excitability. The molecular rearrangement underlying inactivation is thought to involve the intracellular linker between domains III and IV serving as inactivation lid, the receptor for the lid (domain III S4-S5 linker) and the pore-lining S6 segements. To better understand the role of the domain IV S6 segment in inactivation we performed a cysteine scanning mutagenesis of this region in rNav 1.4 channels and screened the constructs for perturbations in the voltage-dependence of steady state inactivation...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29225974/modulation-effects-of-cordycepin-on-voltage-gated-sodium-channels-in-rat-hippocampal-ca1-pyramidal-neurons-in-the-presence-absence-of-oxygen
#17
Zhi-Bin Liu, Chao Liu, Bin Zeng, Li-Ping Huang, Li-Hua Yao
Our previous study revealed that cordycepin features important neuroprotective effects against hypoxic insult by improvement of neuronal electrophysiological function. Modulation on voltage-gated sodium channel (VGSC) in CA1 neurons is the initial event during hypoxia/ischemia. However, no study comprehensively investigated cordycepin on VGSC. Hence, this study investigated modulation effects of cordycepin on VGSC not only in oxygen physiological conditions but also in acute oxygen deprivation injury conditions...
2017: Neural Plasticity
https://www.readbyqxmd.com/read/29071509/toxins-that-affect-voltage-gated-sodium-channels
#18
Yonghua Ji
Voltage-gated sodium channels (VGSCs) are critical in generation and conduction of electrical signals in multiple excitable tissues. Natural toxins, produced by animal, plant, and microorganisms, target VGSCs through diverse strategies developed over millions of years of evolutions. Studying of the diverse interaction between VGSC and VGSC-targeting toxins has been contributing to the increasing understanding of molecular structure and function, pharmacology, and drug development potential of VGSCs. This chapter aims to summarize some of the current views on the VGSC-toxin interaction based on the established receptor sites of VGSC for natural toxins...
October 26, 2017: Handbook of Experimental Pharmacology
https://www.readbyqxmd.com/read/29071507/posttranslational-modification-of-sodium-channels
#19
Zifan Pei, Yanling Pan, Theodore R Cummins
Voltage-gated sodium channels (VGSCs) are critical determinants of excitability. The properties of VGSCs are thought to be tightly controlled. However, VGSCs are also subjected to extensive modifications. Multiple posttranslational modifications that covalently modify VGSCs in neurons and muscle have been identified. These include, but are not limited to, phosphorylation, ubiquitination, palmitoylation, nitrosylation, glycosylation, and SUMOylation. Posttranslational modifications of VGSCs can have profound impact on cellular excitability, contributing to normal and abnormal physiology...
October 26, 2017: Handbook of Experimental Pharmacology
https://www.readbyqxmd.com/read/29045055/c2-modified-sparteine-derivatives-are-a-new-class-of-potentially-long-acting-sodium-channel-blockers
#20
Vaibhavkumar S Gawali, Svilen Simeonov, Martina Drescher, Thomas Knott, Olaf Scheel, John Kudolo, Hanspeter Kählig, Ulla Hochenegg, Alexander Roller, Hannes Todt, Nuno Maulide
The lupin alkaloid sparteine is a well-known chiral diamine with a range of applications in asymmetric synthesis, as well as a blocker of voltage-gated sodium channels (VGSCs). However, there is only scarce information on the VGSC-blocking activity of sparteine derivatives where the structure of the parent alkaloid is retained. Building on the recent renewed availability of sparteine and derivatives we report herein how modification of sparteine at position 2 produces irreversible blockers of VGSCs. These compounds could be clinically envisaged as long-lasting local anesthetics...
November 22, 2017: ChemMedChem
keyword
keyword
95921
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"