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hair follicle stem cell

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https://www.readbyqxmd.com/read/29923313/establishment-of-an-in-vitro-organoid-model-of-dermal-papilla-of-human-hair-follicle
#1
Abhishak C Gupta, Shikha Chawla, Ashok Hegde, Divya Singh, Balaji Bandyopadhyay, Chandrasekharan C Lakshmanan, Gurpreet Kalsi, Sourabh Ghosh
Human hair dermal papilla (DP) cells are specialized mesenchymal cells that play a pivotal role in hair regeneration and hair cycle activation. The current study aimed to first develop three-dimensional (3D) DP spheroids (DPS) with or without a silk-gelatin (SG) microenvironment, which showed enhanced DP-specific gene expression, resulting in enhanced extracellular matrix (ECM) production compared with a monolayer culture. We tested the feasibility of using this DPS model for drug screening by using minoxidil, which is a standard drug for androgenic alopecia...
June 19, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29901112/mir%C3%A2-339%C3%A2-5p-negatively-regulates-loureirin-a%C3%A2-induced-hair-follicle-stem-cell-differentiation-by-targeting-dlx5
#2
Xiangjun Li, Yuqiong Wu, Fangfang Xie, Fengxue Zhang, Saixia Zhang, Jianhong Zhou, Dongfeng Chen, Aijun Liu
Our previous study indicated that loureirin A induces hair follicle stem cell (HFSC) differentiation through Wnt/β‑catenin signaling pathway activation. However, if and how microRNAs (miRNAs/miRs) modulate loureirin A‑induced differentiation remains to be elucidated. In the present study, HFSCs were separated from the vibrissae of rats and identified by CD34 and keratin, type 1 cytoskeletal (K)15 expression. Microarray‑based miRNA profiling analysis revealed that miR‑339‑5p was downregulated in loureirin A‑induced HFSC differentiation...
May 31, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29899403/fgf-signalling-controls-the-specification-of-hair-placode-derived-sox9-positive-progenitors-to-merkel-cells
#3
Minh Binh Nguyen, Idan Cohen, Vinod Kumar, Zijian Xu, Carmit Bar, Katherine L Dauber-Decker, Pai-Chi Tsai, Pauline Marangoni, Ophir D Klein, Ya-Chieh Hsu, Ting Chen, Marja L Mikkola, Elena Ezhkova
Merkel cells are innervated mechanosensory cells responsible for light-touch sensations. In murine dorsal skin, Merkel cells are located in touch domes and found in the epidermis around primary hairs. While it has been shown that Merkel cells are skin epithelial cells, the progenitor cell population that gives rise to these cells is unknown. Here, we show that during embryogenesis, SOX9-positive (+) cells inside hair follicles, which were previously known to give rise to hair follicle stem cells (HFSCs) and cells of the hair follicle lineage, can also give rise to Merkel Cells...
June 13, 2018: Nature Communications
https://www.readbyqxmd.com/read/29896657/interactions-between-epidermal-keratinocytes-dendritic-epidermal-t-cells-and-hair-follicle-stem-cells
#4
Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, Colin Jamora
The interplay of immune cells and stem cells in maintaining skin homeostasis and repair is an exciting new frontier in cutaneous biology. With the growing appreciation of the importance of this new crosstalk comes the requirement of methods to interrogate the molecular underpinnings of these leukocyte-stem cell interactions. Here we describe how a combination of FACS, cellular coculture assays, and conditioned media treatments can be utilized to advance our understanding of this emerging area of intercellular communication between immune cells and stem cells...
June 13, 2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29895193/melatonin-promotes-cashmere-goat-capra-hircus-secondary-hair-follicle-growth-a-view-from-integrated-analysis-of-long-non-coding-and-coding-rnas
#5
Wei Ge, Shan-He Wang, Bing Sun, Yue-Lang Zhang, Wei Shen, Hasan Khatib, Xin Wang
The role of melatonin in promoting the yield of Cashmere goat wool has been demonstrated for decades though there remains a lack of knowledge regarding melatonin mediated hair follicle growth. Recent studies have demonstrated that long non-coding RNAs (lncRNAs) are widely transcribed in the genome and play ubiquitous roles in regulating biological processes. However, the role of lncRNAs in regulating melatonin mediated hair follicle growth remains unclear. In this study, we established an in vitro Cashmere goat secondary hair follicle culture system, and demonstrated that 500 ng/L melatonin exposure promoted hair follicle fiber growth...
June 12, 2018: Cell Cycle
https://www.readbyqxmd.com/read/29891910/anti-inflammatory-role-of-15-lipoxygenase-contributes-to-the-maintenance-of-skin-integrity-in-mice
#6
Sang-Nam Kim, Seun Akindehin, Hyun-Jung Kwon, Yeon-Ho Son, Abhirup Saha, Young-Suk Jung, Je-Kyung Seong, Kyung-Min Lim, Jong-Hyuk Sung, Krishna Rao Maddipati, Yun-Hee Lee
15-lipoxygenase is involved in the generation of specialized pro-resolving lipid mediators that play essential roles in resolution and inflammatory responses. Here, we investigated anti-inflammatory role of Alox15 in skin homeostasis. We demonstrated that knockout (KO) of Alox15 led to hair loss and disrupted the structural integrity of the dorsal skin. Alox15 KO resulted in loss of hair follicle stem cells and abnormal transition of dermal adipocytes into fibroblasts. Alox15 deficiency increased infiltration of proinflammatory macrophages and upregulated proinflammatory and necroptotic signaling in dermal adipose tissue in the dorsal skin...
June 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29883895/organ-regeneration-based-on-developmental-biology-past-and-future
#7
REVIEW
Makoto Takeo, Takashi Tsuji
In this decade, great progress has been made in the field of organ regeneration by incorporating emerging concepts from the fields of stem cell biology and developmental biology, and this progress has pioneered a new frontier in regenerative medicine. The generation of bioengineered organ germ-utilizing, fate-determined, organ-inductive epithelial and mesenchymal cells has provided evidence for the concept of functional organ regeneration in vivo. Organoid studies have verified that nearly all organs can be generated in the form of a mini-organ by recapitulating embryonic body patterning and establishing an organ-forming field among self-organizing pluripotent stem cells by utilizing cytokines that mimic the patterning and positional signals of organogenesis...
June 5, 2018: Current Opinion in Genetics & Development
https://www.readbyqxmd.com/read/29805107/mouse-intestinal-krt15-crypt-cells-are-radio-resistant-and-tumor-initiating
#8
Véronique Giroux, Julien Stephan, Priya Chatterji, Ben Rhoades, E Paul Wileyto, Andres J Klein-Szanto, Christopher J Lengner, Kathryn E Hamilton, Anil K Rustgi
Two principal stem cell pools orchestrate the rapid cell turnover in the intestinal epithelium. Rapidly cycling Lgr5+ stem cells are intercalated between the Paneth cells at the crypt base (CBCs) and injury-resistant reserve stem cells reside above the crypt base. The intermediate filament Keratin 15 (Krt15) marks either stem cells or long-lived progenitor cells that contribute to tissue repair in the hair follicle or the esophageal epithelium. Herein, we demonstrate that Krt15 labels long-lived and multipotent cells in the small intestinal crypt by lineage tracing...
June 5, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29797272/development-of-hair-fibres
#9
Duane P Harland, Jeffrey E Plowman
The growth of hairs occurs during the anagen phase of the follicle cycle. Hair growth begins with basement membrane-bound stem cells (mother cells) around the dermal papilla neck which continuously bud off daughter cells which further divide as a transient amplifying population. Division ceases as cell line differentiation begins, which entails changes in cell junctions, cell shape and position, and cell-line specific cytoplasmic expression of keratin and trichohyalin. As the differentiating cells migrate up the bulb, nuclear function ceases in cortex, cuticle and inner root sheath (IRS) layers...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29771334/%C3%AE-catenin-activation-in-hair-follicle-dermal-stem-cells-induces-ectopic-hair-outgrowth-and-skin-fibrosis
#10
Yixin Tao, Qingchun Yang, Lei Wang, Jie Zhang, Xuming Zhu, Qianqian Sun, Yunbin Han, Qian Luo, Yushu Wang, Xizhi Guo, Ji Wu, Baojie Li, Xiao Yang, Lin He, Gang Ma
Hair follicle dermal sheath (DS) harbors hair follicle dermal stem cells (hfDSCs), which can be recruited to replenish DS and dermal papilla (DP). Cultured DS cells can differentiate into various cell lineages in vitro. However, it is unclear how its plasticity is modulated in vivo. Wnt/β-catenin signaling plays an important role in maintaining stem cells of various lineages and is required for HF development and regeneration. Here we report that activation of β-catenin in DS generates ectopic HF outgrowth (EF) by reprogramming HF epidermal cells and DS cells themselves, and endows DS cells with hair inducing ability...
May 16, 2018: Journal of Molecular Cell Biology
https://www.readbyqxmd.com/read/29765409/a-novel-secretory-vesicle-from-deer-antlerogenic-mesenchymal-stem-cell-conditioned-media-damsc-cm-promotes-tissue-regeneration
#11
Minkoo Seo, Jin-Chul Kim, Hyung-Ki Kim, Eun Wook Choi, Suyeong Jeong, Ki Chang Nam, Mihue Jang
Multipotent stem cells have the capacity to generate terminally differentiated cell types of each lineage; thus, they have great therapeutic potential for a wide variety of diseases. The most widely available stem cells are derived from human tissues, and their use for therapeutic application is limited by their high cost and low productivity. Herein, we report that conditioned media of mesenchymal stem cells (MSCs) isolated from deer antlers enhanced tissue regeneration through paracrine action via a combination of secreted growth factors and cytokines...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29764513/cellular-therapy-with-human-autologous-adipose-derived-adult-cells-of-stromal-vascular-fraction-for-alopecia-areata
#12
Rami Anderi, Nehman Makdissy, Albert Azar, Francine Rizk, Aline Hamade
BACKGROUND: Most common forms of hair loss (alopecia) are caused by aberrant hair follicle cycling and changes in hair follicle morphology. However, current treatments for alopecia do not specifically target these processes. Adipose-derived stromal vascular cells (ADSVCs) that can be harvested from fat cells are one of the latest breakthroughs in the aesthetic field. The potential use of stem cell-based therapies (SCBT) for the repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions, which can be applied to prevent hair loss...
May 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29754201/skin-bioprinting-a-novel-approach-for-creating-artificial-skin-from-synthetic-and-natural-building-blocks
#13
Robin Augustine
Significant progress has been made over the past few decades in the development of in vitro-engineered substitutes that mimic human skin, either as grafts for the replacement of lost skin, or for the establishment of in vitro human skin models. Tissue engineering has been developing as a novel strategy by employing the recent advances in various fields such as polymer engineering, bioengineering, stem cell research and nanomedicine. Recently, an advancement of 3D printing technology referred as bioprinting was exploited to make cell loaded scaffolds to produce constructs which are more matching with the native tissue...
May 12, 2018: Progress in Biomaterials
https://www.readbyqxmd.com/read/29752798/review-of-human-hair-optical-properties-in-possible-relation-to-melanoma-development
#14
Xiyong Huang, Michael D Protheroe, Ahmed M Al-Jumaily, Sharad P Paul, Andrew N Chalmers
Immigration and epidemiological studies provide evidence indicating the correlation of high ultraviolet exposure during childhood and increased risks of melanoma in later life. While the explanation of this phenomenon has not been found in the skin, a class of hair has been hypothesized to be involved in this process by transmitting sufficient ultraviolet rays along the hair shaft to possibly cause damage to the stem cells in the hair follicle, ultimately resulting in melanoma in later life. First, the anatomy of hair and its possible contribution to melanoma development, and the tissue optical properties are briefly introduced to provide the necessary background...
May 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29705291/macrophages-promote-wound-induced-hair-follicle-regeneration-in-a-cx-3-cr1-and-tgf%C3%AE-1-dependent-manner
#15
Waleed Rahmani, Yunan Liu, Nicole L Rosin, Adrienne Kline, Eko Raharjo, Jessica Yoon, Jo Anne Stratton, Sarthak Sinha, Jeff Biernaskie
Hair follicle (HF) stem cells are regulated by intrafollicular and extrafollicular niche signals. Appropriate HF regeneration relies on the coordinated release and integration of these signals. How immune cells, particularly cutaneous macrophages, influence the HF stem cell niche and regeneration is not well understood. We took advantage of wound-induced hair growth (WIHG) to explore the relationship between wound macrophages and HF regeneration. First, we show that WIHG is dependent upon CD11b+ F4/80+ macrophages between 7-11 days post-injury...
April 26, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29704117/regeneration-of-mouse-skin-melanocyte-stem-cells-in-vivo-and-in-vitro
#16
Ke Yang, Weiming Qiu, Pei-Rong Gu, Mingxing Lei
Coordinated regeneration of melanocyte stem cells (McSCs) and hair follicle stem cells (HSCs) contributes to generation of pigmented hairs. Synchronous regeneration of McSCs with activation of HSCs occurs not only during initiation of a new hair cycle in vivo but also during reconstitution of hair follicles in vitro. The duration of the quiescent state of these stem cells becomes longer and longer in lifespan of mammals, leading to a decreased regenerative ability to form hair follicles. Here, we describe methods to activate McSCs during hair follicle regeneration in vivo, and isolate melanocytes from neonatal mouse skin to generate an immortalized cell line of melanocyte progenitors in vitro, aiming to use them for studying melanogenesis and future clinical application...
April 28, 2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29700975/use-of-homeobox-gene-expression-patterns-to-determine-anatomical-regions-of-origin-for-body-surface-tissues-derived-from-adult-mice
#17
Toru Okubo, Ryuhei Hayashi, Shun Shibata, Yuji Kudo, Yoichi Honma, Kohji Nishida
Anatomical regions of the skin have distinct functions and anatomical characteristics, including thicker or thinner epidermis, more or fewer hair follicles, and lighter or darker skin. For a better therapeutic outcome of skin transplantation, site-specific characteristics of grafted tissues need to be taken into account in terms of their functionality and beauty. However, there is no method for evaluating positional information of epidermal cells. Homeobox genes are expressed along the anterior-posterior axis and direct the body plan in the animal development process...
April 26, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29682604/the-hair-follicle-an-underutilized-source-of-cells-and-materials-for-regenerative-medicine
#18
Mehrdad T Kiani, Claire A Higgins, Benjamin D Almquist
The hair follicle is one of only two structures within the adult body that selectively degenerates and regenerates, making it an intriguing organ to study and use for regenerative medicine. Hair follicles have been shown to influence wound healing, angiogenesis, neurogenesis, and harbor distinct populations of stem cells; this has led to cells from the follicle being used in clinical trials for tendinosis and chronic ulcers. In addition, keratin produced by the follicle in the form of a hair fiber provides an abundant source of biomaterials for regenerative medicine...
April 9, 2018: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/29672929/functional-anatomy-of-the-hair-follicle-the-secondary-hair-germ
#19
REVIEW
Andrey A Panteleyev
The secondary hair germ (SHG) - a transitory structure in the lower portion of the mouse telogen hair follicle (HF) - is directly involved in anagen induction and eventual HF regrowth. Some crucial aspects of SHG functioning and ontogenetic relations with other HF parts, however, remain undefined. According to recent evidence (in contrast to previous bulge-centric views), the SHG is the primary target of anagen-inducing signaling and a source of both the outer root sheath (ORS) and ascending HF layers during the initial (morphogenetic) anagen sub-phase...
April 19, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29661566/lichen-planopilaris-and-frontal-fibrosing-alopecia-as-model-epithelial-stem-cell-diseases
#20
REVIEW
Matthew J Harries, Francisco Jimenez, Ander Izeta, Jonathan Hardman, Sreejith Parameswara Panicker, Enrique Poblet, Ralf Paus
Inflammation-associated, irreversible damage to epithelial stem cells (eSCs) of the hair follicle in their immunologically privileged niche lies at the heart of scarring alopecia, which causes permanent difficult-to-treat hair loss. We propose that the two most common and closely related forms, lichen planopilaris (LPP) and frontal fibrosing alopecia (FFA), provide excellent model diseases for studying the biology and pathology of adult human eSCs in an easily accessible human mini-organ. Emphasising the critical roles for interferon (IFN)-γ and peroxisome proliferator-activated receptor (PPAR)-γ-mediated signalling in immune privilege (IP) collapse and epithelial-mesenchymal transition (EMT) of these eSCs respectively, we argue that these pathways deserve therapeutic targeting in the future management of LPP/FFA and other eSC diseases associated with IP collapse and EMT...
May 2018: Trends in Molecular Medicine
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