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https://www.readbyqxmd.com/read/27905816/cementocyte-cell-death-occurs-in-rat-cellular-cementum-during-orthodontic-tooth-movement
#1
Humihiro Matsuzawa, Naoko Toriya, Yuya Nakao, Moe Konno-Nagasaka, Toshiya Arakawa, Miki Okayama, Itaru Mizoguchi
OBJECTIVE: To clarify the mechanism of root resorption during orthodontic treatment, we examined cementocyte cell death and root resorption in the cellular cementum on the pressure side during experimental tooth movement. MATERIALS AND METHODS: Using 8-week-old male Wistar rats, the right first molar was pushed mesiobuccally with a force of 40 g by a Ni-Ti alloy wire while the contralateral first molar was used as a control. Localization and number of cleaved caspase-3-positive and single-stranded DNA (ssDNA) - positive cells were evaluated using dual-label immunohistochemistry with anticleaved caspase-3 and anti-ssDNA antibodies...
December 1, 2016: Angle Orthodontist
https://www.readbyqxmd.com/read/27905295/desensitization-of-idiopathic-pulmonary-fibrosis-fibroblasts-to-alternaria-alternata-extract-mediated-necrotic-cell-death
#2
Jintaek Im, Kyutae Kim, Ji Young Yhee, Scott M O'Grady, Richard S Nho
Alternaria alternata is an allergenic fungus and known to cause an upper respiratory tract infection and asthma in humans with compromised immunity. Although A. alternata's effect on airway epithelial cells has previously been examined, the potential role of A. alternata on lung fibroblast viability is not understood. Since lung fibroblasts derived from patients with idiopathic pulmonary fibrosis (IPF) display a distinct phenotype that is resistant to stress and cell death inducing conditions, the investigation of the role of Alternaria on pathological IPF fibroblasts provides a better understanding of the fibrotic process induced by an allergenic fungus...
November 2016: Physiological Reports
https://www.readbyqxmd.com/read/27904697/inhibitory-effect-of-bufalin-on-retinoblastoma-cells-hxo-rb44-via-the-independent-mitochondrial-and-death-receptor-pathway
#3
Qingfeng Meng, Yan Zhao, LiXin An, Xia Li, Ping Liu
Cinobufacini (Huachansu) is a Chinese medicine prepared from the skin of Bufo bufo gargarizans Cantor (Bufonidae), and has long been used in traditional Chinese medicine. In the present study, the anti-retinoblastoma constituent bufalin obtained from Cinobufacini was investigated. Treatment of human retinoblastoma (HXO-RB44) cells with bufalin induced apoptosis which was accompanied by a decrease in mitochondrial membrane potential, activation of caspase-9, caspase-8 and caspase-3, as well as changes in the expression of cytochrome C...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904667/icaritin-induces-apoptotic-and-autophagic-cell-death-in-human-glioblastoma-cells
#4
Zhaopei Li, Xiangwen Meng, Lin Jin
BACKGROUND: GBM represents the most aggressive type of glioma which is featured by extremely aggressive invasion and destructive malignancy with a high proliferation rate. The aim of this study was to investigate the in vitro anti-tumor effect of icaritin in human GBM cell line U87. METHODS: The effect of icaritin on In vitro cell viability was determined by MTT assay and colony formation assay. The inducing effect of icaritin on cell cycle arrest, mitochondrial membrane potential loss, apoptosis, autophagy and intracellular ROS generation was assessed by flow cytometry...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27904666/ad-hgf-improves-the-cardiac-remodeling-of-rat-following-myocardial-infarction-by-upregulating-autophagy-and-necroptosis-and-inhibiting-apoptosis
#5
Jiabao Liu, Peng Wu, Yunle Wang, Yingqiang Du, Nan A, Shuiyuan Liu, Yiming Zhang, Ningtian Zhou, Zhihui Xu, Zhijian Yang
Cell death in MI is the most critical determinant of subsequent left ventricular remodeling and heart failure. Besides apoptosis, autophagy and necroptosis have been recently found to be another two regulated cell death styles. HGF has been reported to have a protective role in MI, but its impact on the three death styles remains unclear. Thus, our study was performed to investigate the distribution of autophagy, apoptosis and necroptosis in cardiac tissues after MI and explore the role and mechanism of Ad-HGF on cardiac remodeling by regulating the three death styles...
2016: American Journal of Translational Research
https://www.readbyqxmd.com/read/27902334/the-ns1-protein-of-avian-influenza-virus-h9n2-induces-oxidative-stress-mediated-chicken-oviduct-epithelial-cells-coecs-apoptosis
#6
Xuefeng Qi, Huizhu Zhang, Qiuzhen Wang, Jingyu Wang
The pathogenesis of H9N2 subtype avian influenza virus infection (AIV) in the hens is often related to the oviduct tissue damage. The viral nonstructural NS1 protein is thought to play key role in regulating the pathogenicity of AIV, but the exact function to this process remains elusive. In this study, the pro-apoptosis effect of H9N2 NS1 protein was examined on chicken oviduct epithelial cells (COECs) and our data suggested that NS1 induced oxidative stress was a contributing factor for apoptosis. Our data suggest that NS1 protein level was correlated with reactive oxygen species (ROS) in COECs cells transfected with NS1 expression plasmids...
October 20, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27900057/in-vitro-and-in-vivo-inhibition-of-tumor-cell-viability-by-combined-dihydroartemisinin-and-doxorubicin-treatment-and-the-underlying-mechanism
#7
Xiang Tai, Xiao-Bei Cai, Zhang Zhang, Rui Wei
The natural extract artemisinin and its derivatives have good anticancer activity. The present study aimed to investigate the in vitro inhibitory effects of combined dihydroartemisinin (DHA) and doxorubicin (DOX) treatment on a variety of tumor cell lines (HeLa, OVCAR-3, MCF-7, PC-3 and A549), as well as the underlying mechanisms. In addition, the in vivo effects of DHA and DOX were evaluated using a mouse HeLa tumor model. The HeLa, OVCAR-3, MCF-7, PC-3 and A549 cells were treated with a combination of DHA and DOX, and the effect on cell viability was detected by Cell Counting kit-8...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27899859/effect-of-n-perfluorooctane-on-hypoxia-reoxygenation-injury-in-human-umbilical-vein-endothelial-cells
#8
Jing Jiang, Deng-Yong Chen, Zhi-Tao Liu, Feng Chen, Jing-Jing Zhang, Jing Cui, Jien Pang
BACKGROUND: This study investigated the effect of n-perfluorooctane (PFC) on hypoxia/reoxygenation (H/R) injury in human umbilical vein endothelial cells (HUVECs). METHODS: In this study, the H/R models were prepared by chemical methods (using dithionite solution). The experimental groups included the control group, the PFC group with a culture volume ratio of 10%, the H/R model group, and treatment groups with various doses of PFC + H/R (i.e., 5%, 10%, or 20% PFC by volume)...
November 2016: Acta Cardiologica Sinica
https://www.readbyqxmd.com/read/27899257/juglanin-induces-apoptosis-and-autophagy-in-human-breast-cancer-progression-via-ros-jnk-promotion
#9
Zhu-Lei Sun, Jin-Long Dong, Jiang Wu
Breast cancer is one of the most common primary malignant tumors of among women, the long-term survival of which has stagnated in the past decades. Juglanin as a natural production mainly extracted from green walnut husks of Juglans mandshurica has been defined as the functional composition among a series of compounds. It showed powerful protective effect in various diseases by inhibiting inflammation and tumor cells growth. However, the effect of juglanin on human breast cancer and the underlying mechanisms remains to be elucidated...
November 26, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27896224/in-vitro-apoptotic-effects-of-farnesyltransferase-blockade-in-acute-myeloid-leukemia-cells
#10
V Giudice, P Ricci, L Marino, M Rocco, G Villani, M Langella, L Manente, E Seneca, I Ferrara, L Pezzullo, B Serio, C Selleri
Farnesyltransferase inhibitors (FTIs) are a class of oral anti-cancer drugs currently tested in phase I-II clinical trials for treatment of hematological malignancies. The in vitro effects of various FTIs (alpha-hydroxyfarnesylphosphonic acid, manumycin-A and SCH66336) were tested on CD34(+) KG1a cell line and in primary acute myeloid leukemia (AML) cells from 64 patients. By cell viability and clonogeneic methylcellulose assays, FTIs showed a significant inhibitory activity in CD34(+) KG1a and primary bone marrow (BM) leukemic cells from 56% of AML patients...
November 2016: Translational Medicine @ UniSa
https://www.readbyqxmd.com/read/27895893/downregulation-of-long-non-coding-rna-h19-promotes-p19cl6-cells-proliferation-and-inhibits-apoptosis-during-late-stage-cardiac-differentiation-via-mir-19b-modulated-sox6
#11
Yu Han, Hongdang Xu, Jiangtao Cheng, Yanwei Zhang, Chuanyu Gao, Taibing Fan, Bangtian Peng, Bin Li, Lin Liu, Zhaoyun Cheng
BACKGROUND: Regulating cardiac differentiation to maintain normal heart development and function is very important. At present, biological functions of H19 in cardiac differentiation is not completely clear. METHODS: To explore the functional effect of H19 during cardiac differentiation. Expression levels of early cardiac-specific markers Nkx-2.5 and GATA4, cardiac contractile protein genes α-MHC and MLC-2v were determined by qRT-PCR and western lot. The levels of lncRNA H19 and miR-19b were detected by qRT-PCR...
2016: Cell & Bioscience
https://www.readbyqxmd.com/read/27894079/microrna-214-protects-against-hypoxia-reoxygenation-induced-cell-damage-and-myocardial-ischemia-reperfusion-injury-via-suppression-of-pten-and-bim1-expression
#12
Xiaohui Wang, Tuanzhu Ha, Yuanping Hu, Chen Lu, Li Liu, Xia Zhang, Race Kao, John Kalbfleisch, David Williams, Chuanfu Li
BACKGROUND: Myocardial apoptosis plays an important role in myocardial ischemia/reperfusion (I/R) injury. Activation of PI3K/Akt signaling protects the myocardium from I/R injury. This study investigated the role of miR-214 in hypoxia/reoxygenation (H/R)-induced cell damage in vitro and myocardial I/R injury in vivo. METHODS AND RESULTS: H9C2 cardiomyoblasts were transfected with lentivirus expressing miR-214 (LmiR-214) or lentivirus expressing scrambled miR-control (LmiR-control) respectively, to establish cell lines of LmiR-214 and LmiR-control...
November 22, 2016: Oncotarget
https://www.readbyqxmd.com/read/27890875/induction-of-apoptosis-by-ethanol-extract-of-evodia-rutaecarpa-in-hela-human-cervical-cancer-cells-via-activation-of-amp-activated-protein-kinase
#13
Seon Young Park, Cheol Park, Shin-Hyung Park, Su-Hyun Hong, Gi-Young Kim, Sang Hoon Hong, Yung-Hyun Choi
The fruit of Evodia rutaecarpa (Juss.) Benth has been used widely in traditional medicine therapy. Although it has been shown to possess many pharmacological activities, the molecular mechanisms of its anti-cancer activity have not been clearly elucidated. In the present study, we investigated the pro-apoptotic effects of an ethanol extract isolated from immature fruits of E. rutaecarpa (EEER) in HeLa human cervical cancer cells. EEER treatment decreased the cell viability of HeLa cells in a concentration-dependent manner, which was related to apoptotic cell death resulting from apoptotic body formation, DNA fragmentation, and an increased population of annexin V(+)-positive cells...
November 27, 2016: Bioscience Trends
https://www.readbyqxmd.com/read/27889234/in-silico-and-in-vitro-anti-cancer-potential-of-a-curcumin-analogue-1e-6e-1-7-di-1h-indol-3-yl-hepta-1-6-diene-3-5-dione
#14
Shamim Akhtar Sufi, Lakshmi Narayana Adigopula, Safiulla Basha Syed, Victor Mukherjee, Mohane S Coumar, H Surya Prakash Rao, Rukkumani Rajagopalan
PURPOSE: Previously we showed that BDMC, an analogue of curcumin suppresses growth of human breast and laryngeal cancer cell line by causing apoptosis. Here, we demonstrate the enhanced anti-cancer activity of a heterocyclic ring (indole) incorporated curcumin analogue ((1E, 6E)-1, 7-di (1H-indol-3-yl) hepta-1, 6-diene-3, 5-Dione), ICA in short, in comparison to curcumin. METHOD: ICA was synthesized by a one pot condensation reaction. Anti-cancer potential of ICA was assessed in three human cancer cell lines of different origin (Lung adenocarcinoma (A549), leukemia (K562) and colon cancer (SW480)) by MTT assay...
November 23, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27888812/a-polysaccharide-from-lentinus-edodes-inhibits-human-colon-cancer-cell-proliferation-and-suppresses-tumor-growth-in-athymic-nude-mice
#15
Jinglin Wang, Weiyong Li, Xiao Huang, Ying Liu, Qiang Li, Ziming Zheng, Kaiping Wang
The antitumor effect of Lentinan is thought rely on the activation of immune responses; however, little is known about whether Lentinan also directly attacks cancer cells. We therefore investigated the direct antitumor activity of SLNT (a water-extracted polysaccharide from Lentinus edodes) and its probable mechanism. We showed that SLNT significantly inhibited proliferation of HT-29 colon cancer cells and suppressed tumor growth in nude mice. Annxein V-FITC/PI, DAPI, AO/EB and H&E staining assays all showed that SLNT induced cell apoptosis both in vitro and in vivo...
November 21, 2016: Oncotarget
https://www.readbyqxmd.com/read/27888799/new-bipyridine-gold-iii-dithiocarbamate-containing-complexes-exerted-a-potent-anticancer-activity-against-cisplatin-resistant-cancer-cells-independent-of-p53-status
#16
Muhammad Altaf, Muhammad Monim-Ul-Mehboob, Abdel-Nasser Kawde, Giuseppe Corona, Roberto Larcher, Marcia Ogasawara, Naike Casagrande, Marta Celegato, Cinzia Borghese, Zahid H Siddik, Donatella Aldinucci, Anvarhusein A Isab
We synthesized, characterized and tested in a panel of cancer cell lines, nine new bipyridine gold(III) dithiocarbamate-containing complexes. In vitro studies demonstrated that compounds 1, 2, 4, 5, 7 and 8 were the most cytotoxic in prostate, breast, ovarian cancer cell lines and in Hodgkin lymphoma cells with IC50 values lower than the reference drug cisplatin. The most active compound 1 was more active than cisplatin in ovarian (A2780cis and 2780CP-16) and breast cancer cisplatin-resistant cells. Compound 1 determined an alteration of the cellular redox homeostasis leading to increased ROS levels, a decrease in the mitochondrial membrane potential, cytochrome-c release from the mitochondria and activation of caspases 9 and 3...
November 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27888447/the-regulation-of-the-mitochondrial-apoptotic-pathway-by-glucocorticoid-receptor-in-collaboration-with-bcl-2-family-proteins-in-developing-t-cells
#17
Lilla Prenek, Ferenc Boldizsár, Réka Kugyelka, Emese Ugor, Gergely Berta, Péter Németh, Timea Berki
Glucocorticoids (GC) are important in the regulation of selection and apoptosis of CD4(+)CD8(+) double-positive (DP) thymocytes. The pronounced GC-sensitivity of DP thymocytes, observed earlier, might be due to the combination of classical (genomic) and alternative (non-genomic) glucocorticoid receptor (GR) signaling events modifying activation or apoptotic pathways. In particular, the previously demonstrated mitochondrial translocation of activated GR in DP thymocytes offered a fascinating explanation for their pronounced GC-induced apoptosis sensitivity...
November 26, 2016: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/27888130/protective-role-of-phenylalanine-on-the-ros-induced-oxidative-damage-apoptosis-and-tight-junction-damage-via-nrf2-tor-and-nf-%C3%AE%C2%BAb-signalling-molecules-in-the-gill-of-fish
#18
Lin Feng, Wen Li, Yang Liu, Wei-Dan Jiang, Sheng-Yao Kuang, Jun Jiang, Ling Tang, Yong-An Zhang, Xiao-Qiu Zhou
This study explored the possible preventive effects of dietary phenylalanine (Phe) on antioxidant responses, apoptosis and tight junction protein transcription in the gills of young grass carp (Ctenopharyngodon idella). Fish were fed six different experimental diets containing graded levels of Phe (3.4-16.8 g kg(-1)) for 8 weeks. The results showed that Phe deficiency induced protein oxidation and lipid peroxidation by decreasing the glutathione content and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) in fish gill (P < 0...
November 22, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27886059/regulation-of-intrinsic-and-extrinsic-apoptotic-pathways-in-osteosarcoma-cells-following-oleandrin-treatment
#19
Yunlong Ma, Bin Zhu, Lei Yong, Chunyu Song, Xiao Liu, Huilei Yu, Peng Wang, Zhongjun Liu, Xiaoguang Liu
Our previous study has reported the anti-tumor effect of oleandrin on osteosarcoma (OS) cells. In the current study, we mainly explored its potential regulation on intrinsic and extrinsic apoptotic pathway in OS cells. Cells apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were detected using fluorescence staining and flow cytometry. Caspase-3 activity was detected using a commercial kit. The levels of cytoplasmic cytochrome c, mitochondrial cytochrome c, bcl-2, bax, caspase-9, Fas, FasL, caspase-8 and caspase-3 were detected by Western blotting...
November 23, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27884396/effects-of-visfatin-on-the-apoptosis-of-intestinal-mucosal-cells-in-immunological-stressed-rats
#20
Ying Zhou, Huai-Rui Yuan, Lu Cui, Abdur Rahman Ansari, Ke Xiao, You Luo, Xin-Tong Wu, Liang Guo, Faheem Ahmed Khan, Zhi Yang, Hui Song
This study was undertaken to determine if visfatin is involved in the inflammation or apoptosis introduced by LPS in rats. Forty 8-week old Wistar rats were divided into four groups (n=10 in each group) and injected with saline, visfatin, LPS and visfatin+LPS co-stimulated via caudal vein. The duodenum, jejunum and ileum were harvested from all the rats. Compared to the saline treated group, visfatin significantly increased the number of TUNEL-positive apoptotic cells and the expression of caspase-3 protein in intestinal mucosa...
November 21, 2016: Acta Histochemica
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