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Kung-Ha Choi, Mi Hwa Park, Hyun Ah Lee, Ji-Sook Han
Exposure to high levels of glucose may cause glucotoxicity, leading to pancreatic β cell dysfunction, including cell apoptosis and impaired glucose-stimulated insulin secretion. The aim of this study was to explore the effect of cyanidin-3-rutinoside (C3R), a derivative of anthocyanin, on glucotoxicity-induced apoptosis in INS-1 pancreatic β cells. Glucose (30 mM) treatment induced INS-1 pancreatic β cell death, but glucotoxicity and apoptosis significantly decreased in cells treated with 50 μM C3R compared to that observed in 30 mM glucose-treated cells...
June 20, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
Kristina Henz, Aoula Al-Zebeeby, Marion Basoglu, Simone Fulda, Gerald M Cohen, Shankar Varadarajan, Meike Vogler
Induction of apoptosis by selective BH3-mimetics is currently investigated as a novel strategy for cancer treatment. Here, we report that selective BH3-mimetics induce apoptosis in a variety of hematological malignancies. Apoptosis is accompanied by severe mitochondrial toxicities upstream of caspase activation. Specifically, the selective BH3-mimetics ABT-199, A-1331852 and S63845, which target BCL-2, BCL-XL and MCL-1, respectively, induce comparable ultrastructural changes including mitochondrial swelling, a decrease of mitochondrial matrix density and severe loss of cristae structure...
June 1, 2018: Biological Chemistry
Hany E A Ahmed, Mohammed A A El-Nassag, Ahmed H Hassan, Rawda M Okasha, Saleh Ihmaid, Ahmed M Fouda, Tarek H Afifi, Ateyatallah Aljuhani, Ahmed M El-Agrody
In our effort to develop novel and powerful agents with anti-proliferative activity, two new series of 1H-benzo[f]chromene derivatives, 4a-h and 6a-h, were synthesised using heterocyclocondensation methodologies under microwave irradiation condition. The structures of the target compounds were established on the basis of their spectral data, IR, 1 H NMR, 13  C NMR, 13  C NMR-DEPT/APT, and MS data. The new compounds have been examined for their anti-proliferative activity against three cancer cell lines, MCF-7, HCT-116, and HepG-2...
December 2018: Journal of Enzyme Inhibition and Medicinal Chemistry
Yinping Tong, Gongye Zhang, Yang Li, Jiajia Xu, Jiahui Yuan, Bing Zhang, Tianhui Hu, Gang Song
Corilagin is a component of Phyllanthus urinaria extract and has been found of possessing anti-inflammatory, anti-oxidative, and anti-tumour properties in clinic treatments. However, the underlying mechanisms in anti-cancer particularly of its induction of cell death in human breast cancer remain undefined. Our research found that corilagin-induced apoptotic and autophagic cell death depending on reactive oxygen species (ROS) in human breast cancer cell, and it occurred in human breast cancer cell (MCF-7) only comparing with normal cells...
June 19, 2018: Journal of Cellular and Molecular Medicine
Sellamani Muthulakshmi, Pourmohammadi Fallah Hamideh, Hamid R Habibi, Kannan Maharajan, Krishna Kadirvelu, Venkataramana Mudili
In the present study, we aimed to assess the adverse effects of zearalenone (ZEA) at environmentally relevant concentrations (0.5, 1, 5 and 10 μg l-1 ) on hypothalamic-pituitary-gonadal axis associated reproductive function using zebrafish model. ZEA was exposed to female zebrafish for 21 days to assess growth indices such as condition factor, hepatosomatic index, gonadosomatic index and caspase 3 activity. Further, expression of estrogen receptor (ER) α and CYP19a1b genes in the brain, ERα and vitellogenin (Vtg) genes in the liver and follicle-stimulating hormone receptor, luteinizing hormone receptor, ERα, steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase (HSD), 17-βHSD and CYP19a1 genes in the ovary were also investigated...
June 19, 2018: Journal of Applied Toxicology: JAT
K Nishida, T Matsushita, K Takayama, T Tanaka, N Miyaji, K Ibaraki, D Araki, N Kanzaki, T Matsumoto, R Kuroda
Objectives: This study aimed to examine the effects of SRT1720, a potent SIRT1 activator, on osteoarthritis (OA) progression using an experimental OA model. Methods: Osteoarthritis was surgically induced by destabilization of the medial meniscus in eight-week-old C57BL/6 male mice. SRT1720 was administered intraperitoneally twice a week after surgery. Osteoarthritis progression was evaluated histologically using the Osteoarthritis Research Society International (OARSI) score at four, eight, 12 and 16 weeks...
March 2018: Bone & Joint Research
Elif Sinem Iplik, Baris Ertugrul, Ilknur Kozanoglu, Yusuf Baran, Bedia Cakmakoglu
Objectives: Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer. Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies...
May 2018: Iranian Journal of Basic Medical Sciences
Jie Yu, Hongwei Gao, Chuanhong Wu, Qiong-Ming Xu, Jin-Jian Lu, Xiuping Chen
Doxorubicin (DOX) is a widely used antineoplastic agent in clinics. However, its clinical application is largely limited by its cardiotoxicity. Diethyl blechnic (DB) is a novel compound isolated from Salvia miltiorrhiza Bunge. Here, we study the effect of DB on DOX-induced cardiotoxicity and its underlying mechanisms. Cellular viability was tested by 3-[-4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and protein level was evaluated by Western blotting. 5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining was performed to determine the mitochondrial membrane potential (MMP)...
June 19, 2018: International Journal of Molecular Sciences
Chun-Gu Wang, Wei-Na Yao, Bin Zhang, Jing Hua, Dong Liang, Heng-Shan Wang
Cancer metastasis has been a major impediment to effective cancer treatment and is the major cause of cancer-related death. Polyphenols compounds have been reported to possess anti-metastasis activity through their inhibitory activity of matrix metalloproteinases (MMPs). In this paper, twelve polyphenols compounds, including three novel phenols, named selaphenins A-C (1-3), two known selaginellin derivatives (4 and 5), together with seven known biflavonoids (6-12) were isolated from the plant of Selaginella tamariscina...
June 17, 2018: Bioorganic & Medicinal Chemistry Letters
W Tang, J Mu, Q I Chen, X Li, H Liu
It has been proved that hyperuricemia is associated with non-alcoholic fatty liver disease (NAFLD). The xanthine oxidase (XO) inhibitor, febuxostat, decreases free fatty acids-induced fat accumulation in HFDT-fed mice. Here, it is shown that febuxostat attenuates fat accumulation and reactive oxygen species (ROS) in HepG2 cells. It was further found that the underlying mechanism is related to the reduction in expression of NLRP3/caspase-1/IL-18/IL-1beta and improved insulin resistance (IR). This finding highlights the possible molecular pathways involving NLRP3 activation for management of ROS and insulin IR...
May 2018: Journal of Biological Regulators and Homeostatic Agents
Ziyan Yu, Wenting Yu, Jia Liu, Danhong Wu, Chunxia Wang, Jinsong Zhang, Jiangyue Zhao
Objective To investigate the influence of Msx2 conditional gene knockout during lens development in mice. Methods Lens-specific Msx2 knockout mice were generated using the Cre-loxP system. The eyes of Msx2 conditional knockout ( Msx2CKO) and wild-type ( Msx2WT) mice were examined during embryonic and early postnatal periods using histological, immunofluorescence, in situ hybridization, cell proliferation, apoptosis, and mRNA microarray analyses. Results Msx2CKO mice exhibited small lens formation and microphthalmia after birth, while Msx2CKO embryos exhibited a persistent lens stalk, small lens formation, and microphthalmia...
January 1, 2018: Journal of International Medical Research
Veysel T Yilmaz, Ceyda Icsel, Omer R Turgut, Muhittin Aygun, Merve Erkisa, Mehmet H Turkdemir, Engin Ulukaya
A series of new Pt(II) saccharinate complexes containing PR3 ligands (PPh3 , PPh2 Cy, PPhCy2 and PCy3 ) with progressive phenyl (Ph) replacement by cyclohexyl (Cy) were synthesized and structurally characterized by IR, NMR, ESI-MS and X-ray diffraction. The anticancer activity of the complexes was tested against human breast (MCF-7), lung (A549), colon (HCT116), and prostate (DU145) cancer cell lines as well as against normal bronchial epithelial (BEAS-2B) cells. Trans-configured complexes 1, 3 and 5 emerged as potential anticancer drug candidates...
June 16, 2018: European Journal of Medicinal Chemistry
Kariyappa Vijay, Poorigali Raghavendra-Rao Sowmya, Bangalore Prabhashankar Arathi, Shivaprasad Shilpa, Hulikere Jagdish Shwetha, Marisiddaiah Raju, Vallikannan Baskaran, Rangaswamy Lakshminarayana
The combination of carotenoids and doxorubicin (DOX) selectively alters oxidative stress-mediated apoptosis in breast cancer cells. Primarily, cytotoxic efficiency of carotenoids (β-carotene, BC; lutein, LUT; astaxanthin, AST; or fucoxanthin, FUCO) either with or without a minimal cytotoxic dose of DOX was evaluated in MCF-7 (0.12 μM) and MDA-MB-231 cells (0.28 μM). The higher cell growth inhibition of BC and/or LUT with DOX was selected for testing in further cell-based assays. Low-dose DOX significantly enhanced cytotoxicity in carotenoid (<5 μM)-treated cells compared to high-dose DOX (>1 μM) or carotenoid (20 μM) treatment alone...
June 18, 2018: Food and Chemical Toxicology
Mohammad-Reza Khajevand-Khazaei, Parvaneh Mohseni-Moghaddam, Marjan Hosseini, Leila Gholami, Tourandokht Baluchnejadmojarad, Mehrdad Roghani
Acute kidney injury (AKI) is a common complication following severe sepsis, its incidence is increasing, and it is associated with a high rate of morbidity and mortality. Rutin is a glycoside of the bioflavonoid quercetin with various protective effects due to its antioxidant and anti-inflammatory potential. In this research, we tried to assess the protective effect of rutin administration in a model of AKI in C57BL/6 mice. For induction of AKI, lipopolysaccharide (LPS) was injected once (10mg/kg, i.p.) and rutin was p...
June 16, 2018: European Journal of Pharmacology
Weiwei Dai, William P Miller, Allyson L Toro, Adam J Black, Sadie K Dierschke, Robert P Feehan, Scot R Kimball, Michael D Dennis
The role of dyslipidemia in the development of retinal dysfunction remains poorly understood. Using an animal model of diet-induced obesity/pre-type 2 diabetes, we investigated molecular defects in the retina arising from consumption of a diet high in saturated fats and sugars ( i.e., a Western diet). We found that feeding mice a Western diet increased the abundance of retinal sphingolipids, attenuated protein kinase B (Akt) phosphorylation, enhanced JNK activation, and increased retinal cell death. When we used palmitate or C6-ceramide (Cer) to assess sphingolipid-mediated signaling in cultured murine and human cells, we observed similar effects on Akt, JNK, and cell death...
June 19, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Xiao-Xin Chen, Kar Ho Lam, Yibin Feng, Kai Xu, Stephen C W Sze, Chi Wai Tang, George P H Leung, Calvin Kai-Fai Lee, Jun Shi, Zhijun Yang, Sheng-Tao Li, Zhang-Jin Zhang, Yanbo Zhang
Worldwide, colorectal cancer (CRC) is a deleterious disease causing millions of death annually. 5-Fluorouracil (5-FU) is a first-line chemotherapy for CRC, but chemoresistance and gastrointestinal mucositis limit its efficacy. Polyphenol-rich foods are increasingly popular due to their potential beneficial role in cancer. Ellagitannins is a group of phenolic compounds commonly found in pomegranate, strawberries, raspberries, etc. The objective of this study was to explore whether ellagitannins from pomegranate (PETs) could ameliorate 5-FU-induced intestinal mucositis and enhance its efficacy against CRC...
June 19, 2018: Journal of Agricultural and Food Chemistry
H-B Zheng, Y-T Fu, G Wang, L-H Sun, Y-Y Fan, T-W Yang
OBJECTIVE: We aimed to explore the effect of hyperphosphorylation of Tau on cognitive function of propofol-anesthetized rats. MATERIALS AND METHODS: Thirty 2-month-old male Wistar rats weighing 180-220 g were randomly divided into 3 groups (n=10): group of treating with saline (C group), group of treating with propofol for 1 hour (P1) and 24 h (P24 group). The cognitive function of rats was tested by Morris water maze before and 1 h or 24 h after drug administration...
June 2018: European Review for Medical and Pharmacological Sciences
L-J Hou, J-J Han, Y Liu
OBJECTIVE: The present study is aimed to investigate the regulatory effect of microRNA (miRNA or miR)-503 on endothelial functions, as well as the mechanism by which high glucose leads to injury of endothelial cells. MATERIALS AND METHODS: When reaching 80% confluency, human umbilical vein endothelial cells (HUVECs) were subjected to non-serum synchronization for 12 h, and medium of cells in high-glucose (HG) group was replaced by normal medium supplemented with 25 mmol/L D-glucose...
June 2018: European Review for Medical and Pharmacological Sciences
S-Y Chang, R-Q Sun, M Feng, Y-X Li, H-L Wang, Y-M Xu
OBJECTIVE: The effect of lipoxin receptor agonist BML-111 on acute kidney injury and AKI in rats with hemorrhagic shock (HS) and its mechanism were investigated. MATERIALS AND METHODS: A model of hemorrhagic shock in Sprague-Dawley (SD) rats was established, and recovered after 30 min of shock. 1 mg/kg BML-111 was intraperitoneally injected at the beginning of resuscitation in group BML-111. The concentration of serum creatinine, serum KIM-1 content, NGAL and inflammatory factors were detected...
June 2018: European Review for Medical and Pharmacological Sciences
Eun Ji Ryu, Dae Won Kim, Min Jea Shin, Hyo Sang Jo, Jung Hwan Park, Su Bin Cho, Chi Hern Lee, Hyeon Ji Yeo, Eun Ji Yeo, Yeon Joo Choi, Duk-Soo Kim, Sung-Woo Cho, Yong-Jun Cho, Eun Jeong Sohn, Ora Son, Keun Wook Lee, Kyu Hyung Han, Jinseu Park, Won Sik Eum, Soo Young Choi
Oxidative stress is known to be a primary risk factor for neuronal diseases. Glutaredoxin (GLRX)‑1, a redox‑regulator of the thioredoxin superfamily, is known to exhibit an important role in cell survival via various cellular functions. However, the precise roles of GLRX1 in brain ischemia are still not fully understood. The present study investigated whether transduced PEP‑1‑GLRX1 protein has protective effects against oxidative stress in cells and in an animal model. Transduced PEP‑1‑GLRX1 protein increased HT‑22 cell viability under oxidative stress and this fusion protein significantly reduced intracellular reactive oxygen species and levels of DNA damage...
June 15, 2018: Molecular Medicine Reports
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