keyword
MENU ▼
Read by QxMD icon Read
search

label free biomolecular interaction

keyword
https://www.readbyqxmd.com/read/29139285/subradiant-dipolar-interactions-in-plasmonic-nanoring-resonator-array-for-integrated-label-free-biosensing
#1
Yuzhang Liang, Hui Zhang, Wenqi Zhu, Amit Agrawal, Henri J Lezec, Lixia Li, Wei Peng, Yi Zou, Yan-Qing Lu, Ting Xu
With the development of advanced nanofabrication technologies over the last decade, plasmonic nanostructures have attracted wide attention for their potential in label-free biosensing applications. However, the sensing performance of nanostructured plasmonic sensors is primarily limited by the broad-linewidth features with low peak-to-dip signal ratio in the extinction spectra that result from strong radiative damping. Here, we propose and systematically investigate the in-plane and out-of-plane dipolar interactions in an array of plasmonic nanoring resonators that are from the spatial combination of classic nanohole and nanodisk structures...
November 15, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29092176/dual-kretschmann-and-otto-configuration-fiber-surface-plasmon-resonance-biosensor
#2
Lixia Li, Yuzhang Liang, Jianye Guang, Wenli Cui, Xinpu Zhang, Jean-Francois Masson, Wei Peng
We present a dual-resonance fiber surface plasmon resonance (SPR) sensor for biological analysis. The sensing element was fabricated by sequentially sputtering layers of indium tin oxide (ITO) (100 nm thickness) and Au (35 nm thickness) on the surface of an optical fiber. The refractive index dispersion effect of ITO material led to resonances in the near infrared and visible wavelength regions. The refractive index of ITO is larger than the optical fiber in visible spectral area (400 to 733nm), such that the structure is a typical Kretschmann configuration surface plasmon resonance sensor...
October 30, 2017: Optics Express
https://www.readbyqxmd.com/read/29053253/surface-plasmon-resonance-material-and-interface-design-for-universal-accessibility
#3
Samuel S Hinman, Kristy S McKeating, Quan Cheng
No abstract text is available yet for this article.
November 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28970921/near-uv-visible-electronic-absorption-originating-from-charged-amino-acids-in-a-monomeric-protein
#4
Saumya Prasad, Imon Mandal, Shubham Singh, Ashim Paul, Bhubaneswar Mandal, Ravindra Venkatramani, Rajaram Swaminathan
Electronic absorption spectra of proteins are primarily characterized over the ultraviolet region (185-320 nm) of the electromagnetic spectrum. While recent studies on peptide aggregates have revealed absorption beyond 350 nm, monomeric proteins lacking aromatic amino acids, disulphide bonds, and active site prosthetic groups are expected to remain optically silent beyond 250 nm. Here, in a joint theoretical and experimental investigation, we report the distinctive UV-Vis absorption spectrum between 250 nm [ε = 7338 M(-1) cm(-1)] and 800 nm [ε = 501 M(-1) cm(-1)] in a synthetic 67 residue protein (α3C), in monomeric form, devoid of aromatic amino acids...
August 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28945239/focal-molography-is-a-new-method-for-the-in-situ-analysis-of-molecular-interactions-in-biological-samples
#5
Volker Gatterdam, Andreas Frutiger, Klaus-Peter Stengele, Dieter Heindl, Thomas Lübbers, Janos Vörös, Christof Fattinger
Focal molography is a next-generation biosensor that visualizes specific biomolecular interactions in real time. It transduces affinity modulation on the sensor surface into refractive index modulation caused by target molecules that are bound to a precisely assembled nanopattern of molecular recognition sites, termed the 'mologram'. The mologram is designed so that laser light is scattered at specifically bound molecules, generating a strong signal in the focus of the mologram via constructive interference, while scattering at nonspecifically bound molecules does not contribute to the effect...
November 2017: Nature Nanotechnology
https://www.readbyqxmd.com/read/28929734/label-free-and-high-throughput-detection-of-biomolecular-interactions-using-a-flatbed-scanner-biosensor
#6
Ugur Aygun, Oguzhan Avci, Elif Seymour, Hakan Urey, M Selim Ünlü, Ayca Yalcin Ozkumur
Fluorescence based microarray detection systems provide sensitive measurements; however, variation of probe immobilization and poor repeatability negatively affect the final readout, and thus quantification capability of these systems. Here, we demonstrate a label-free and high-throughput optical biosensor that can be utilized for calibration of fluorescence microarrays. The sensor employs a commercial flatbed scanner, and we demonstrate transformation of this low cost (∼100 USD) system into an Interferometric Reflectance Imaging Sensor through hardware and software modifications...
October 27, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28924921/mg-2-induced-dna-compaction-condensation-and-phase-separation-in-gene-delivery-vehicles-based-on-zwitterionic-phospholipids-a-dynamic-light-scattering-and-surface-enhanced-raman-spectroscopic-study
#7
Erhan Süleymanoğlu
Despite the significant efforts towards applying improved non-destructive and label-free measurements of biomolecular structures of lipid-based gene delivery vectors, little is achieved in terms of their structural relevance in gene transfections. Better understanding of structure-activity relationships of lipid-DNA complexes and their gene expression efficiencies thus becomes an essential issue. Raman scattering offers a complimentary measurement technique for following the structural transitions of both DNA and lipid vesicles employed for their transfer...
December 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28880211/dual-mode-electro-optical-techniques-for-biosensing-applications-a-review
#8
REVIEW
José Juan-Colás, Steven Johnson, Thomas F Krauss
The monitoring of biomolecular interactions is a key requirement for the study of complex biological processes and the diagnosis of disease. Technologies that are capable of providing label-free, real-time insight into these interactions are of great value for the scientific and clinical communities. Greater understanding of biomolecular interactions alongside increased detection accuracy can be achieved using technology that can provide parallel information about multiple parameters of a single biomolecular process...
September 7, 2017: Sensors
https://www.readbyqxmd.com/read/28814800/variations-in-biocorona-formation-related-to-defects-in-the-structure-of-single-walled-carbon-nanotubes-and-the-hyperlipidemic-disease-state
#9
Achyut J Raghavendra, Kristofer Fritz, Sherleen Fu, Jared M Brown, Ramakrishna Podila, Jonathan H Shannahan
Ball-milling utilizes mechanical stress to modify properties of carbon nanotubes (CNTs) including size, capping, and functionalization. Ball-milling, however, may introduce structural defects resulting in altered CNT-biomolecule interactions. Nanomaterial-biomolecule interactions result in the formation of the biocorona (BC), which alters nanomaterial properties, function, and biological responses. The formation of the BC is governed by the nanomaterial physicochemical properties and the physiological environment...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28750539/octupolar-metastructures-for-a-highly-sensitive-rapid-and-reproducible-phage-based-detection-of-bacterial-pathogens-by-surface-enhanced-raman-scattering
#10
Massimo Rippa, Riccardo Castagna, Marianna Pannico, Pellegrino Musto, Giorgia Borriello, Rubina Paradiso, Giorgio Galiero, Sergio Bolletti Censi, Jun Zhou, Joseph Zyss, Lucia Petti
The development of fast and ultrasensitive methods to detect bacterial pathogens at low concentrations is of high relevance for human and animal health care and diagnostics. In this context, surface-enhanced Raman scattering (SERS) offers the promise of a simplified, rapid, and high-sensitive detection of biomolecular interactions with several advantages over previous assay methodologies. In this work, we have conceived reproducible SERS nanosensors based on tailored multilayer octupolar nanostructures which can combine high enhancement factor and remarkable molecular selectivity...
July 28, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28703788/preparation-of-a-trp-bodipy-fluorogenic-amino-acid-to-label-peptides-for-enhanced-live-cell-fluorescence-imaging
#11
Lorena Mendive-Tapia, Ramon Subiros-Funosas, Can Zhao, Fernando Albericio, Nick D Read, Rodolfo Lavilla, Marc Vendrell
Fluorescent peptides are valuable tools for live-cell imaging because of the high specificity of peptide sequences for their biomolecular targets. When preparing fluorescent versions of peptides, labels must be introduced at appropriate positions in the sequences to provide suitable reporters while avoiding any impairment of the molecular recognition properties of the peptides. This protocol describes the preparation of the tryptophan (Trp)-based fluorogenic amino acid Fmoc-Trp(C2-BODIPY)-OH and its incorporation into peptides for live-cell fluorescence imaging-an approach that is applicable to most peptide sequences...
August 2017: Nature Protocols
https://www.readbyqxmd.com/read/28448040/determination-of-high-affinity-antibody-antigen-binding-kinetics-using-four-biosensor-platforms
#12
Danlin Yang, Ajit Singh, Helen Wu, Rachel Kroe-Barrett
Label-free optical biosensors are powerful tools in drug discovery for the characterization of biomolecular interactions. In this study, we describe the use of four routinely used biosensor platforms in our laboratory to evaluate the binding affinity and kinetics of ten high-affinity monoclonal antibodies (mAbs) against human proprotein convertase subtilisin kexin type 9 (PCSK9). While both Biacore T100 and ProteOn XPR36 are derived from the well-established Surface Plasmon Resonance (SPR) technology, the former has four flow cells connected by serial flow configuration, whereas the latter presents 36 reaction spots in parallel through an improvised 6 x 6 crisscross microfluidic channel configuration...
April 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28364249/surface-plasmon-resonance-based-concentration-determination-assay-label-free-and-antibody-free-quantification-of-morpholinos
#13
Jordan Boutilier, Hong M Moulton
Surface plasmon resonance (SPR) is a physical process that allows label-free and real-time detection of biomolecular interactions. SPR provides a rapid and quantitative method for studying interactions of macromolecules such as proteins and nucleic acids. Antisense Morpholino oligomers are widely used to regulate gene expression and the US FDA has approved a Morpholino drug for treatment of Duchenne muscular dystrophy. Here, we describe an antibody-free, label-free, high-throughput, and walk-away SPR method for quantification of Morpholino compounds extracted from biological specimens...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28340485/an-approach-for-liposome-immobilization-using-sterically-stabilized-micelles-ssms-as-a-precursor-for-bio-layer-interferometry-based-interaction-studies
#14
Jakob Wallner, Gabriele Lhota, Markus Schosserer, Karola Vorauer-Uhl
Non-fluidic bio-layer interferometry (BLI) has rapidly become a standard tool for monitoring almost all biomolecular interactions in a label-free, real-time and high-throughput manner. High-efficiency screening methods which measure the kinetics of liposomes with a variety of compounds require the immobilization of liposomes. In this work, a method is described for immobilizing liposomes for interaction studies, based on the biophysical principles of this biosensor platform. The immobilization approach includes the loading of DSPE-PEG(2000)-biotin containing sterically stabilized micelles (SSMs) which are restructured in a buffer change step, resulting in an accessible substrate for liposome immobilization...
June 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28327660/quantitative-volumetric-raman-imaging-of-three-dimensional-cell-cultures
#15
Charalambos Kallepitis, Mads S Bergholt, Manuel M Mazo, Vincent Leonardo, Stacey C Skaalure, Stephanie A Maynard, Molly M Stevens
The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment...
March 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28281256/label-free-biosensors-based-on-bimodal-waveguide-bimw-interferometers
#16
Sonia Herranz, Adrián Fernández Gavela, Laura M Lechuga
The bimodal waveguide (BiMW) sensor is a novel common path interferometric transducer based on the evanescent field detection principle, which in combination with a bio-recognition element allows the direct detection of biomolecular interactions in a label-free scheme. Due to its inherent high sensitivity it has great potential to become a powerful analytical tool for monitoring substances of interest in areas such as environmental control, medical diagnostics and food safety, among others. The BiMW sensor is fabricated using standard silicon-based technology allowing cost-effective production, and meeting the requirements of portability and disposability necessary for implementation in a point-of-care (POC) setting...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28225259/one-step-ligand-immobilization-and-single-sample-injection-for-regeneration-free-surface-plasmon-resonance-measurements-of-biomolecular-interactions
#17
Xiaoying Wang, Zhiqiang Li, Nguyen Ly, Feimeng Zhou
Surface plasmon resonance (SPR) has been well established as a method of choice for label-free kinetic measurements of biomolecular interactions. The conventional approach involves multiple injections of an analyte of different concentrations into a fluidic channel covered with a fixed ligand density. Optimization of the experimental conditions and assessment of the data quality can be complicated by issues such as disruption of the ligand structure by the regeneration step and the limited availability of the sample solution...
March 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28067047/solvatochromic-and-fluorogenic-dyes-as-environment-sensitive-probes-design-and-biological-applications
#18
Andrey S Klymchenko
Fluorescent environment-sensitive probes are specially designed dyes that change their fluorescence intensity (fluorogenic dyes) or color (e.g., solvatochromic dyes) in response to change in their microenvironment polarity, viscosity, and molecular order. The studies of the past decade, including those of our group, have shown that these molecules become universal tools in fluorescence sensing and imaging. In fact, any biomolecular interaction or change in biomolecular organization results in modification of the local microenvironment, which can be directly monitored by these types of probes...
January 9, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/27924486/surface-plasmon-resonance-a-useful-strategy-for-the-identification-of-small-molecule-argonaute-2-protein-binders
#19
Elena Poser, Ilaria Genovese, Silvia Masciarelli, Teresa Bellissimo, Francesco Fazi, Gianni Colotti
Surface plasmon resonance (SPR) is one of the most important techniques for the detection and the characterization of molecular interactions. SPR technology is a label-free approach for monitoring biomolecular interactions in real time. The binding of analytes to molecules immobilized on a thin metal film (ligand) determines a change in the refractive index and, therefore in the angle of extinction of light, is reflected when polarized light hits the film, monitored in real time as a change in the position of the dip in reflected intensity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27888307/monitoring-doxorubicin-cellular-uptake-and-trafficking-using-in-vitro-raman-microspectroscopy-short-and-long-time-exposure-effects-on-lung-cancer-cell-lines
#20
Zeineb Farhane, Franck Bonnier, Hugh J Byrne
Raman microspectroscopy is a non-invasive, in vitro analytical tool which is being increasingly explored for its potential in clinical applications and monitoring the uptake, mechanism of action and cellular interaction at a molecular level of chemotherapeutic drugs, ultimately as a potential label-free preclinical screening and companion diagnostic tool. In this study, doxorubicin (DOX), a "gold standard" chemotherapeutic drug, is employed as a model in the in vitro lung cancer cell line A549 in order to demonstrate the potential of Raman microspectroscopy to screen and identify spectroscopic markers of its trafficking and mechanism of action...
November 25, 2016: Analytical and Bioanalytical Chemistry
keyword
keyword
95913
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"