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M M Dowsey, O Robertsson, M Sundberg, L S Lohmander, P F M Choong, A W-Dahl
OBJECTIVE: Preoperative pain and function is viewed as an important predictor of total knee arthroplasty (TKA) outcomes. We examined whether variations in pain and function outcomes existed at 12 months between two centres in Sweden and Australia, and whether this was explained by variations in patient presentation for TKA. METHODS: This was a retrospective analysis of prospectively collected data. Patients from one centre in Australia (St. Vincent's Hospital (SVH), N = 516) and in Sweden (TGB, N = 899) who underwent primary TKA between 2012 and 2013...
December 20, 2016: Osteoarthritis and Cartilage
Tsai-Ling Ho, Hsiang-Chi Lee, Yuan-Lin Chou, Yang-Hao Tseng, Wei-Cheng Huang, Chiung-Hua Wung, Na-Sheng Lin, Yau-Heiu Hsu, Ban-Yang Chang
The movement of some plant viruses are accomplished by three proteins encoded by a triple gene block (TGB). The second protein (TGBp2) in the block is a transmembrane protein. This study was aimed to unravel the mechanism underlying the relatively inefficient cell-to-cell movement of Bamboo mosaic virus (BaMV) caused by amino acid substitutions for the three Cys residues, Cys-109, Cys-112 and Cys-119, at the C-terminal tail of TGBp2. Results from confocal microscopy revealed that substitutions of the three Cys residues of TGBp2, especially Cys-109 and Cys-112, would reduce the efficiency of TGBp2- and TGBp3-dependent PD localization of TGBp1...
January 15, 2017: Virology
Jorge A M Rezende, Viviana M Camelo-García, Sónia C S Andrade, José E Buriolla, Elliot W Kitajima, Ligia M L Duarte
In this work, we report the complete genome sequence of, production of polyclonal antibodies against, and development of biological assays for a putative new potexvirus, named senna mosaic virus (SenMV), found infecting Senna occidentalis in the state of São Paulo, Brazil. The complete genome sequence of SenMV comprises 6775 nucleotides excluding the poly(A) tail. The genome organization is similar to those of other potexviruses, with five open reading frames coding for RNA-dependent RNA polymerase (RdRp), the triple gene block (TGB 1, 2, and 3) proteins, and coat protein (CP)...
February 2017: Archives of Virology
Vediappen Padmini, Palakurthy Nani Babu, Geetha G Nair, D S Shankar Rao, Channabasaveshwar V Yelamaggad
Sixteen optically active, non-symmetric dimers, in which cyanobiphenyl and salicylaldimine mesogens are interlinked by a flexible spacer, were synthesized and characterized. While the terminal chiral tail, in the form of either (R)-2-octyloxy or (S)-2-octyloxy chain attached to salicylaldimine core, was held constant, the number of methylene units in the spacer was varied from 3 to 10 affording eight pairs of (R & S) enantiomers. They were probed for their thermal properties with the aid of orthoscopy, conoscopy, differential scanning calorimetry and X-ray powder diffraction...
September 6, 2016: Chemistry, An Asian Journal
Gillian Barry, Paul van Schaik, Alasdair MacSween, John Dixon, Denis Martin
BACKGROUND: The use of exergaming is a potential alternative to traditional methods of balance training, which can be repetitive and somewhat monotonous. The purpose of this study was to assess the effects of exergaming using XBOX Kinect™ versus traditional gym-based exercise with no virtual stimuli (TGB) on postural control, technology acceptance, flow experience and exercise intensity, in young healthy adults. METHODS: Fifty healthy active adults (age: 33.8 ± 12...
2016: BMC Sports Science, Medicine and Rehabilitation
Melissa L Barker-Haliski, Fabiola Vanegas, Matthew J Mau, Tristan K Underwood, H Steve White
OBJECTIVE: Some antiseizure drugs (ASDs) are associated with cognitive liability in patients with epilepsy, thus ASDs without this risk would be preferred. Little comparative pharmacology exists with ASDs in preclinical models of cognition. Few pharmacologic studies exist on the acute effects in rodents with chronic seizures. Predicting risk for cognitive impact with preclinical models may supply valuable ASD differentiation data. METHODS: ASDs (phenytoin [PHT]; carbamazepine [CBZ]; valproic acid [VPA]; lamotrigine [LTG]; phenobarbital [PB]; tiagabine [TGB]; retigabine [RTG]; topiramate [TPM]; and levetiracetam [LEV]) were administered equivalent to maximal electroshock median effective dose ([ED50]; mice, rats), or median dose necessary to elicit minimal motor impairment (median toxic dose [TD50]; rats)...
September 2016: Epilepsia
Takashi Narihiro, Masaru K Nobu, Hideyuki Tamaki, Yoichi Kamagata, Yuji Sekiguchi, Wen-Tso Liu
The syntrophic degradation of branched-chain fatty acids (BCFAs) such as 2-methylbutyrate and isobutyrate is an essential step in the production of methane from proteins/amino acids in anaerobic ecosystems. While a few syntrophic BCFA-degrading bacteria have been isolated, their metabolic pathways in BCFA and short-chain fatty acid (SCFA) degradation as well as energy conservation systems remain unclear. In an attempt to identify these pathways, we herein performed comparative genomics of three syntrophic bacteria: 2-methylbutyrate-degrading "Syntrophomonas wolfei subsp...
September 29, 2016: Microbes and Environments
Fahimeh Soheilipour, Hassan Fazilaty, Fatemeh Jesmi, William A Gahl, Babak Behnam
BACKGROUND: Thyroxine-binding globulin (TBG) is the main transporter of thyroid hormones in human serum, encoded by the gene TBG (SERPINA7), located in long arm of X-chromosome (Xq21-q22). Deficiency of SERPINA7 (serum protease inhibitor, clade A [alpha-1 antiproteinase, antitrypsin], member 7) leads to inherited TBG deficiency. Several mutations have been reported in the coding and noncoding regions of SERPINA7 in association with TGB deficiency. METHODS: Automated chemiluminescence immunoassays were used to determine TSH, free and total T4 and T3 (fT4, TT4, TT3) and TBG...
September 2016: Molecular Genetics and Metabolism Reports
Ekaterina A Lazareva, Alexander A Lezzhov, Tatiana V Komarova, Sergey Y Morozov, Manfred Heinlein, Andrey G Solovyev
Hibiscus green spot virus (HGSV) is a recently discovered and so far poorly characterized bacilliform plant virus with a positive-stranded RNA genome consisting of three RNA species. Here, we demonstrate that the proteins encoded by the ORF2 and ORF3 in HGSV RNA2 are necessary and sufficient to mediate cell-to-cell movement of transport-deficient Potato virus X in Nicotiana benthamiana. These two genes represent a specialized transport module called 'binary movement block'(BMB), and ORF2 and ORF3 are termed BMB1 and BMB2 genes...
April 26, 2016: Molecular Plant Pathology
Xueyan Liu, Qing Liu, Kangpeng Xiao, Pei Li, Qiong Liu, Xinxin Zhao, Qingke Kong
DNA vaccines are widely used to prevent and treat infectious diseases, cancer and autoimmune diseases; however, their relatively low immunogenicity is an obstacle to their use. In this study, we constructed a novel and universal DNA vaccine vector (pSS898) that can be used to build DNA vaccines against duck enteritis virus (DEV) and other viruses that require DNA vaccines to provide protection. This vaccine vector has many advantages, including innate immunogenicity, efficient nuclear trafficking and resistance to attack from nucleases...
April 15, 2016: Veterinary Microbiology
Reza Ranjbar, Morteza Izadi, Taghi T Hafshejani, Faham Khamesipour
Identifying disease vectors and pathogens is one of the key steps in controlling vector-borne diseases. This study investigated the possible role of house flies (Musca domestica) as vectors in the transmission of Klebsiella pneumoniae in Chaharmahal VA Bakhtiari and Isfahan provinces of Iran. House flies were captured from household kitchens, cattle farms, chicken farms, animal hospitals, human hospitals and slaughterhouses. Isolation of K. pneumoniae from external surfaces and guts of the flies was performed using MacConkey agar (MA) and thioglycollate broth (TGB)...
July 2016: Journal of Infection and Public Health
J D Latorre, X Hernandez-Velasco, L R Bielke, J L Vicente, R Wolfenden, A Menconi, B M Hargis, G Tellez
1. The effects of the dietary inclusion of a Bacillus-based direct-fed microbial (DFM) candidate on digesta viscosity, bacterial translocation, microbiota composition and bone mineralisation were evaluated in broilers consuming rye-based diets. 2. In the present study, control mash rye-based diets (CON) or Bacillus-DFM supplemented diets (TRT) were administered ad libitum to male broilers in three independent experiments. 3. In Experiments 1 and 2 (n = 25/group), liver samples were taken to evaluate bacterial translocation, digesta samples were used for viscosity measurements and the intestinal microbial flora was evaluated from different intestinal sections to enumerate total recovered gram-negative bacteria (TGB), lactic acid bacteria (LAB) and anaerobic bacteria (TAB)...
2015: British Poultry Science
Krzysztof Łukawski, Agnieszka Janowska, Stanisław J Czuczwar
BACKGROUND: Losartan and telmisartan, angiotensin AT1 receptor antagonists, are widely used antihypertensive drugs in patients. It is also known that arterial hypertension is often present in people with epilepsy, therefore, drug interactions between AT1 receptor antagonists and antiepileptic drugs can occur in clinical practice. OBJECTIVES: The aim of the current study was to assess the effect of losartan and telmisartan on the anticonvulsant activity of tiagabine, a second-generation antiepileptic drug, in mice...
July 2015: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Alexander A Lezzhov, Vladimir A Gushchin, Ekaterina A Lazareva, Valery K Vishnichenko, Sergey Y Morozov, Andrey G Solovyev
Triple gene block (TGB), a conserved gene module found in the genomes of many filamentous and rod-shaped plant viruses, encodes three proteins, TGB1, TGB2 and TGB3, required for viral cell-to-cell movement through plasmodesmata and systemic transport via the phloem. The genome of Shallot virus X, the type species of the genus Allexivirus, includes TGB1 and TGB2 genes, but contains no canonical ORF for TGB3 protein. However, a TGB3-like protein-encoding sequence lacking an AUG initiator codon has been found in the shallot virus X (ShVX) genome in a position typical for TGB3 genes...
October 2015: Journal of General Virology
Craig T Archbold, Edward J Davis, Richard J Mandle, Stephen J Cowling, John W Goodby
Herein we report studies on a bimesogen that exhibits a chiral twist bend nematic phase when doped with small weight percentages of a chiral material and a partial phase diagram constructed. At low concentrations a wide temperature range blue phase is observed, whereas at higher concentrations an additional 'nematic-like' mesophase was discovered at a lower temperature than the twist-bend nematic phase. In addition to an apparent isotropic-"isotropic" transition, the doped materials also exhibited a weakly birefringent phase upon annealing in the isotropic liquid phase, implying pretransitional behaviour in the same vein as that seen for TGB phases...
October 14, 2015: Soft Matter
Sergey Y Morozov, Andrey G Solovyev
Recently, we hypothesized that silencing suppression activity gained by a viral replicative helicase led to the emergence of the second helicase possessing activity of the viral silencing suppressor and/or movement protein (MP). Our hypothesis accounted for the evolutionary origin of the specialized 'triple gene block' (TGB) in plant virus genomes encoding the MPs TGB1, TGB2, and TGB3 required for viral cell-to-cell transport through plasmodesmata. Here, we used public transcriptome databases to identify previously unrecognized viruses...
2015: Frontiers in Microbiology
Yue Hu, Zhenggang Li, Cheng Yuan, Xuejiao Jin, Lijie Yan, Xiaofei Zhao, Yongliang Zhang, Andrew O Jackson, Xianbing Wang, Chenggui Han, Jialin Yu, Dawei Li
The barley stripe mosaic virus (BSMV) triple gene block 1 (TGB1) protein is required for virus cell-to-cell movement. However, little information is available about how these activities are regulated by post-translational modifications. In this study, we showed that the BSMV Xinjiang strain TGB1 (XJTGB1) is phosphorylated in vivo and in vitro by protein kinase CK2 from barley and Nicotiana benthamiana. Liquid chromatography tandem mass spectrometry analysis and in vitro phosphorylation assays demonstrated that Thr-401 is the major phosphorylation site of the XJTGB1 protein, and suggested that a Thr-395 kinase docking site supports Thr-401 phosphorylation...
August 2015: Journal of Experimental Botany
Claudio Bellevicine, Elena Vigliar, Umberto Malapelle, Emanuele Carelli, Alfonso Fiorelli, Giovanni Vicidomini, Salvatore Cappabianca, Mario Santini, Giancarlo Troncone
Lung adenocarcinoma and papillary thyroid carcinoma (PTC) share a number of microscopic and immunophenotypical features. Thus, patients presenting with thyroid and lung synchronous neoplasms may be difficult on fine-needle aspiration (FNA) samples to define the site of origin of the malignancy. In the case reported here, inherent to a 57-years-old man presenting with a right lung mass and a large (44 mm) thyroid nodule, an integrated cytological, immunocytochemical and molecular approach enabled to clarify the primary nature of the neoplasm...
July 2015: Diagnostic Cytopathology
Hale Ocak, Belkız Bilgin-Eran, Dilek Güzeller, Marko Prehm, Carsten Tschierske
4-Cyanoresorcinol derived bent-core molecules with a chiral (S)-2-methylbutoxy chain form liquid crystalline phases with TGBA- and TGBC-like structures at the transition from cybotactic nematic via SmA to SmC phases.
May 1, 2015: Chemical Communications: Chem Comm
Magdi Yacoub, Mark Radford
A landmark study by Habashi et al(1) in 2006 documented for the first time both the prevention and reversal of structural changes in the aorta associated with Marfan syndrome, via pharmacological means. This study, carried out in a rat model, concluded that such results were due to an inhibitor effect by the drug losartan on TGB-β1 (Figure 1). Habashi's paper prompted some physicians, in the absence of human trials, to begin the clinical off-label use of losartan on Marfan patients, arguing that this was justified due to the drug's excellent safety profile...
2014: Global Cardiology Science & Practice
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