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Mtt assay for antibiofilm activity

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https://www.readbyqxmd.com/read/27864804/activity-of-norspermidine-on-bacterial-biofilms-of-multidrug-resistant-clinical-isolates-associated-with-persistent-extremity-wound-infections
#1
Anthony P Cardile, Ronald L Woodbury, Carlos J Sanchez, Sandra C Becerra, Rebecca A Garcia, Katrin Mende, Joseph C Wenke, Kevin S Akers
Biofilm formation is a major virulence factor for numerous pathogenic bacteria and is cited as a central event in the pathogenesis of chronic human infections, which is in large part due to excessive extracellular matrix secretion and metabolic changes that occur within the biofilm rendering them highly tolerant to antimicrobial treatments. Polyamines, including norspermidine, play central roles in bacterial biofilm development, but have also recently been shown to inhibit biofilm formation in select strains of various pathogenic bacteria...
November 19, 2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27766027/capping-agent-dependent-toxicity-and-antimicrobial-activity-of-silver-nanoparticles-an-in-vitro-study-concerns-about-potential-application-in-dental-practice
#2
Karolina Niska, Narcyz Knap, Anna Kędzia, Maciej Jaskiewicz, Wojciech Kamysz, Iwona Inkielewicz-Stepniak
Objectives: In dentistry, silver nanoparticles (AgNPs) have drawn particular attention because of their wide antimicrobial activity spectrum. However, controversial information on AgNPs toxicity limited their use in oral infections. Therefore, the aim of the present study was to evaluate the antibacterial activities against a panel of oral pathogenic bacteria and bacterial biofilms together with potential cytotoxic effects on human gingival fibroblasts of 10 nm AgNPs: non-functionalized - uncapped (AgNPs-UC) as well as surface-functionalized with capping agent: lipoic acid (AgNPs-LA), polyethylene glycol (AgNPs-PEG) or tannic acid (AgNPs-TA) using silver nitrate (AgNO3) as control...
2016: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/27565090/antibacterial-and-antibiofilm-activities-of-docosahexaenoic-acid-dha-and-eicosapentaenoic-acid-epa-against-periodontopathic-bacteria
#3
Mengjun Sun, Zichao Zhou, Jiachen Dong, Jichun Zhang, Yiru Xia, Rong Shu
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two major omega-3 polyunsaturated fatty acids (n-3 PUFAs) with antimicrobial properties. In this study, we evaluated the potential antibacterial and antibiofilm activities of DHA and EPA against two periodontal pathogens, Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum). MTT assay showed that DHA and EPA still exhibited no cytotoxicity to human oral tissue cells when the concentration came to 100 μM and 200 μM, respectively...
October 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27321770/biofilm-inhibitory-effect-of-chlorhexidine-conjugated-gold-nanoparticles-against-klebsiella-pneumoniae
#4
Ayaz Ahmed, Anum Khalid Khan, Ayaz Anwar, Syed Abid Ali, Muhammad Raza Shah
Klebsiella pneumoniae (K. pneumoniae) is one of the major pathogen associated with nosocomial infections, especially catheter associated urinary tract infections which involved biofilm formation. This study was designed to evaluate the antibiofilm efficacy of gold nanoparticle conjugated with chlorhexidine (Au-CHX) against K. pneumoniae isolates. Au-CHX was synthesized and analyzed for stability by using UV-Visible spectrophotometry, atomic force microscopy (AFM), fourier transform infrared spectroscopy (FT-IR) and electrospray ionization mass spectroscopy (ESI-MS)...
September 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/26669801/bacillomycin-d-and-its-combination-with-amphotericin-b-promising-antifungal-compounds-with-powerful-antibiofilm-activity-and-wound-healing-potency
#5
O Tabbene, S Azaiez, A Di Grazia, I Karkouch, I Ben Slimene, S Elkahoui, M N Alfeddy, B Casciaro, V Luca, F Limam, M L Mangoni
AIMS: In this study, we evaluated the ability of the lipopeptide bacillomycin D and the antifungal drug amphotericin B as well as their combination, to inhibit Candida albicans biofilm formation and to accelerate keratinocyte cell migration. METHODS AND RESULTS: The antibiofilm activity of bacillomycin D and its combination with amphotericin B was carried out by crystal violet colorimetric method. Our results have shown that, when combined together at low concentrations nontoxic to mammalian cells, corresponding to 1/32 MIC (0·39 μg ml(-1) ) and 1/4 MIC (0·06 μg ml(-1) ) for bacillomycin D and amphotericin B, respectively, a clear antibiofilm activity is manifested (95% inhibition of biofilm formation) along with a clear inhibition of germ tube formation...
February 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/25902872/antibacterial-antibiofilm-and-cytotoxic-activities-of-terminalia-fagifolia-mart-extract-and-fractions
#6
Alyne Rodrigues de Araujo, Patrick Veras Quelemes, Márcia Luana Gomes Perfeito, Luíza Ianny de Lima, Melka Coêlho Sá, Paulo Humberto Moreira Nunes, Graziella Anselmo Joanitti, Peter Eaton, Maria José Dos Santos Soares, José Roberto de Souza de Almeida Leite
BACKGROUND: The methicillin resistance of bacteria from the genus Staphylococcus and its ability to form biofilms are important factors in pathogenesis of these microorganisms. Thus, the search for new antimicrobials agents, especially from plants, has been intensified. In this context, Terminalia species have been the subject of research for many pharmacological activities. In this study we evaluated the antibacterial, antibiofilm and cytotoxic activities of the ethanol extract (EtE) from Terminalia fagifolia stem bark as well as that of three fractions of the extract (AqF, HaF and WSF)...
2015: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/24914310/antimicrobial-antibiofilm-and-cytotoxic-activities-of-hakea-sericea-schrader-extracts
#7
Angelo Luís, Luiza Breitenfeld, Susana Ferreira, Ana Paula Duarte, Fernanda Domingues
BACKGROUND: Hakea sericea Schrader is an invasive shrub in Portuguese forests. OBJECTIVE: The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of these extracts, against human cells, were assessed. MATERIALS AND METHODS: The antimicrobial activity of the methanolic extracts of H...
January 2014: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/24224742/effects-of-ambroxol-on-candida-albicans-growth-and-biofilm-formation
#8
Hernandez-Delgadillo Rene, Martínez-Sanmiguel Juan José, Sánchez-Nájera Rosa Isela, Cabral-Romero Claudio
Typically, the onset of candidiasis is characterised by the appearance of a biofilm of Candida albicans, which is associated with several diseases including oral candidiasis in young and elderly people. The objective of this work was to investigate the in vitro fungicidal activity as well as the antibiofilm activity of ambroxol (AMB) against C. albicans growth. In the present investigation, the fungicidal activity of AMB was established using the cell viability 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
April 2014: Mycoses
https://www.readbyqxmd.com/read/24182988/resveratrol-a-potential-inhibitor-of-biofilm-formation-in-vibrio-cholerae
#9
Nimmy Augustine, A K Goel, K C Sivakumar, R Ajay Kumar, Sabu Thomas
Resveratrol, a phytochemical commonly found in the skin of grapes and berries, was tested for its biofilm inhibitory activity against Vibrio cholerae. Biofilm inhibition was assessed using crystal violet assay. MTT assay was performed to check the viability of the treated bacterial cells and the biofilm architecture was analysed using confocal laser scanning microscopy. The possible target of the compound was determined by docking analysis. Results showed that subinhibitory concentrations of the compound could significantly inhibit biofilm formation in V...
February 15, 2014: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/24136453/biosynthesis-of-silver-nanoparticles-from-deep-sea-bacterium-pseudomonas-aeruginosa-jq989348-for-antimicrobial-antibiofilm-and-cytotoxic-activity
#10
V Ramalingam, R Rajaram, C PremKumar, P Santhanam, P Dhinesh, S Vinothkumar, K Kaleshkumar
Pseudomonas aeruginosa (JQ989348) was isolated from deep sea water sample and used for synthesis of silver nanoparticles (AgNPs). AgNPs were confirmed by analyzing surface plasmon resonance using UV-visible spectrophotometer at 420 nm. Further scanning electron microscope analysis confirmed the range of particle size between 13 and 76 nm and XRD pattern authorizes the anisotropic crystalline nature of AgNPs. Fourier transform infrared spectrum endorsed the presence of high amount of proteins and other secondary metabolites in synthesized AgNPs influence the reduction process and stabilization of nanoparticles...
September 2014: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/23643848/effect-of-desensitising-paste-containing-8-arginine-and-calcium-carbonate-on-biofilm-formation-of-streptococcus-mutans-in-vitro
#11
RANDOMIZED CONTROLLED TRIAL
Dongjie Fu, Dandan Pei, Cui Huang, Yinchen Liu, Xijin Du, Hualing Sun
OBJECTIVES: To evaluate the influence of desensitising paste containing 8% arginine and calcium carbonate (Ar-Ca) on biofilm formation on dentine. METHODS: Dentine discs were cut from extracted third molars and divided into the following three groups: no treatment, pumice treatment and Ar-Ca treatment. Surface topography and roughness were examined using scanning electron microscopy (SEM) and non-contact 3D surface profiler. After sterilisation, samples were incubated with Streptococcus mutans (S...
July 2013: Journal of Dentistry
https://www.readbyqxmd.com/read/23600560/an-effective-antibiofilm-agent-against-pseudomonas-aeruginosa-biofilm-from-traditional-thai-herbal-recipes-used-for-wound-treatments
#12
Sasitorn Chusri, Wittaya Jittanon, Katesarin Maneenoon, Supayang Piyawan Voravuthikunchai
The presence of bacterial biofilm, particularly formed by Pseudomonas aeruginosa, has been considered an important factor responsible for wound chronicity. The objective of this study was to investigate the antibiofilm activity of water and ethanol extracts obtained from three traditional herbal recipes (THR-SK004, THR-SK010, and THR-SK011) on biofilm formation and on mature biofilm of a reference strain of P. aeruginosa. The effects of the extracts on the biofilm mass were evaluated by using crystal violet (CV) assay...
October 2013: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/22619529/toxicity-and-antibacterial-assessment-of-chitosan-coated-silver-nanoparticles-on-human-pathogens-and-macrophage-cells
#13
Prajna Jena, Soumitra Mohanty, Rojee Mallick, Biju Jacob, Avinash Sonawane
BACKGROUND: Pathogenic bacteria are able to develop various strategies to counteract the bactericidal action of antibiotics. Silver nanoparticles (AgNPs) have emerged as a potential alternative to conventional antibiotics because of their potent antimicrobial properties. The purpose of this study was to synthesize chitosan-stabilized AgNPs (CS-AgNPs) and test for their cytotoxic, genotoxic, macrophage cell uptake, antibacterial, and antibiofilm activities. METHODS: AgNPs were synthesized using chitosan as both a stabilizing and a reducing agent...
2012: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/21767279/effect-of-cinnamaldehyde-on-biofilm-formation-and-sara-expression-by-methicillin-resistant-staphylococcus-aureus
#14
P Jia, Y J Xue, X J Duan, S H Shao
AIMS: To investigate the antibiofilm effect of cinnamaldehyde on methicillin-resistant Staphylococcus aureus (MRSA) and analyse the effect of subminimum inhibitory concentrations (MICs) of cinnamaldehyde on the expression of the biofilm-related gene sarA. METHODS AND RESULTS: The MICs and minimum bactericidal concentrations (MBCs) were determined using a microtitre broth dilution method. Biofilm susceptibility was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) staining and colony forming unit (CFU) counting assays...
October 2011: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/21630572/antibiofilm-activity-of-selected-plant-essential-oils-and-their-major-components
#15
Aleksandra Budzyńska, Marzena Wieckowska-Szakiel, Beata Sadowska, Danuta Kalemba, Barbara Rózalska
The aim of the study was to examine the antibiofilm activity of selected essential oils (EO): Lavandula angustifblia (LEO), Melaleuca alternifolia (TTO), Melissa officinalis (MEO) and some of their major constituents: linalool, linalyl acetate, alpha-terpineol, terpinen-4-ol. Biofilms were formed by Staphylococcus aureus ATCC 29213 and Escherichia coli NCTC 8196 on the surface of medical biomaterials (urinary catheter, infusion tube and surgical mesh). TTC reduction assay was used for the evaluation of mature biofilm eradication from these surfaces...
2011: Polish Journal of Microbiology
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