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https://www.readbyqxmd.com/read/28101449/immunohistochemical-expression-of-intrarenal-renin-angiotensin-system-components-in-response-to-tempol-in-rats-fed-a-high-salt-diet
#1
Gabriel Cao, Silvana Lorena Della Penna, Nicolás Martín Kouyoumdzian, Marcelo Roberto Choi, Susana Gorzalczany, Belisario Enrique Fernández, Jorge Eduardo Toblli, María Inés Rosón
AIM: To determine the effect of tempol in normal rats fed high salt on arterial pressure and the balance between antagonist components of the renal renin-angiotensin system. METHODS: Sprague-Dawley rats were fed with 8% NaCl high-salt (HS) or 0.4% NaCl (normal-salt, NS) diet for 3 wk, with or without tempol (T) (1 mmol/L, administered in drinking water). Mean arterial pressure (MAP), glomerular filtration rate (GFR), and urinary sodium excretion (UVNa) were measured...
January 6, 2017: World Journal of Nephrology
https://www.readbyqxmd.com/read/28093283/lipophilic-tetraphenylphosphonium-derivatives-enhance-radiation-induced-cell-killing-via-inhibition-of-mitochondrial-energy-metabolism-in-tumor-cells
#2
Hironobu Yasui, Kumiko Yamamoto, Motofumi Suzuki, Yuri Sakai, Tomoki Bo, Masaki Nagane, Eri Nishimura, Tohru Yamamori, Toshihide Yamasaki, Ken-Ichi Yamada, Osamu Inanami
It has recently been reported that radiation enhances mitochondrial energy metabolism in various tumor cell lines. To examine how this radiation-induced alteration in mitochondrial function influences tumor cell viability, various lipophilic triphenylphosphonium (TPP(+)) cation derivatives and related compounds such as 4-hydroxy-2,2,6,6-tetramethyl-1-oxy-piperidin (Tempol) with TPP(+) (named "Mito-") were designed to inhibit the mitochondrial electron transport chain. Mito-(CH2)10-Tempol (M10T) and its derivatives, Mito-(CH2)5-Tempol (M5T), Mito-(CH2)10-Tempol-Methyl (M10T-Me), Mito-C10H21 (M10), and C10H21-Tempol (10T), were prepared...
January 13, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28069660/antioxidant-treatment-attenuates-lactate-production-in-diabetic-nephropathy
#3
Christoffer Laustsen, Per Mose Nielsen, Thomas Stokholm Nørlinger, Haiyun Qi, Uffe Kjærgaard Pedersen, Lotte Bonde Bertelsen, Jakob Appel Østergaard, Allan Flyvbjerg, Jan Henrik Ardenkjær-Larsen, Fredrik Palm, Hans Stødkilde-Jørgensen
The early progression of diabetic nephropathy is notoriously difficult to detect and quantify before the occurrence of substantial histological damage. Recently, hyperpolarized [1-(13)C]pyruvate has demonstrated increased lactate production in the kidney early after the onset of diabetes, implying increased lactate dehydrogenase activity as a consequence of increased nicotinamide adenine dinucleotide substrate availability due to upregulation of the polyol pathway, i.e., pseudohypoxia. In this study, we investigated the role of oxidative stress in mediating these metabolic alterations using state-of-the-art hyperpolarized magnetic resonance (MR) imaging...
January 1, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28064014/gpr55-agonist-lysophosphatidylinositol-and-lysophosphatidylcholine-inhibit-endothelial-cell-hyperpolarization-via-gpr-independent-suppression-of-na-ca-2-exchanger-and-endoplasmic-reticulum-ca-2-refilling
#4
Alexander I Bondarenko, Fabrizio Montecucco, Olga Panasiuk, Vadim Sagach, Nataliya Sidoryak, Karim J Brandt, François Mach
Lysophosphatidylinositol (LPI) and lysophosphatidylcholine (LPC) are lipid signaling molecules that induce endothelium-dependent vasodilation. In addition, LPC suppresses acetylcholine (Ach)-induced responses. We aimed to determine the influence of LPC and LPI on hyperpolarizing responses in vitro and in situ endothelial cells (EC) and identify the underlying mechanisms. Using patch-clamp method, we show that LPI and LPC inhibit EC hyperpolarization to histamine and suppress Na(+)/Ca(2+) exchanged (NCX) currents in a concentration-dependent manner...
January 4, 2017: Vascular Pharmacology
https://www.readbyqxmd.com/read/28063367/effects-of-high-fructose-intake-on-the-development-of-hypertension-in-the-spontaneously-hypertensive-rats-the-role-of-at1r-gp91-phox-signaling-in-the-rostral-ventrolateral-medulla
#5
Kay L H Wu, Chih-Wei Wu, You-Lin Tain, Yung-Mei Chao, Chun-Ying Hung, Pei-Chia Tsai, Wei-Sing Wang, Cheng-Dean Shih
Both genetic and dietary factors determine the development of hypertension. Whether dietary factor impacts the development of hereditary hypertension is unknown. Here, we evaluated the effect of daily high-fructose diet (HFD) on the development of hypertension in adolescent spontaneously hypertensive rats (SHR). Six-week-old SHR were randomly divided into two groups to receive HFD or normal diet (ND) for 3 weeks. The temporal profile of systolic blood pressure, alongside the sympathetic vasomotor activity, in the SHR-HFD showed significantly greater increases at 9-12 weeks of age compared with the age-matched SHR-ND group...
December 5, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28052871/myoglobin-facilitates-angiotensin-ii-induced-constriction-of-renal-afferent-arterioles
#6
Zhi Zhao Liu, Susanne Mathia, Tamara Margit Pahlitzsch, Inggrid Christine Wennysia, Pontus B Persson, En Yin Lai, Anica Högner, Min Ze Xu, Rudolf Schubert, Christian Rosenberger, Andreas Patzak
Vasoconstriction plays an important role in the development of acute kidney injury in rhabdomyolysis. We hypothesized that myoglobin enhances the angiotensin II (Ang II) response in afferent arterioles by increasing superoxide and reducing nitric oxide (NO) bioavailability. Afferent arterioles of C57Bl6 mice were isolated perfused and vasoreactivity was analyzed using video microscopy. NO bioavailability, superoxide concentration in the vessels wall, and changes in cytosolic calcium were measured using fluorescence techniques...
January 4, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28042546/tempol-effect-on-epithelial-mesenchymal-transition-induced-by-hyperglycemia
#7
Mohammad Jafari, Farahnaz Dadras, Hamid Reza Ghadimipour, Mohamad Ali Seif Rabiei, Farhad Khoshjou
BACKGROUND: One of common mechanisms in pathophysiology of chronic kidney diseases is epithelial-mesenchymal transition (EMT). On the other hand oxidative stress has been known to participate in kidney damage of diabetic nephropathy (DN). OBJECTIVES: We studied if tempol, a well-known antioxidant agent, can ameliorate EMT in DN induced in male rats. MATERIALS AND METHODS: Twenty-seven male rats were equally divided in to 4 groups. Group I (control or C), group II (diabetic or D), group III (T) rats which was given tempol (100 mg/kg/day) by gavages for 28 days and group IV (D&T) was diabetic rats that also received same dose of tempol...
January 2017: Journal of Nephropathology
https://www.readbyqxmd.com/read/28039404/free-radical-scavenging-decreases-endothelin-1-excretion-and-glomerular-albumin-permeability-during-type-1-diabetes
#8
Mohamed A Saleh, Carmen De Miguel, David I Stevens, Pamela K Carmines, David M Pollock, Jennifer S Pollock
Increased renal endothelin-1 (ET-1) production and an ETA receptor-dependent increase in glomerular albumin permeability (Palb) accompany type 1 diabetes mellitus (T1D). We hypothesized that T1D-induced oxidative stress contributes to renal ET-1 production and glomerular Palb Male rats with streptozotocin-induced T1D were provided free access to drinking water without additives (T1D rats) or containing the free radical scavenger tempol (1 mmol/L; T1D+Tempol). After 3 weeks, T1D+Tempol rats displayed lower urinary excretion of thiobarbituric acid reactive substances and glomerular superoxide production (dihydroethidium staining) compared to T1D rats...
December 2016: Physiological Reports
https://www.readbyqxmd.com/read/28036148/altered-distribution-of-adrenergic-constrictor-responses-contributes-to-skeletal-muscle-perfusion-abnormalities-in-metabolic-syndrome
#9
Kent Lemaster, Dwayne Jackson, Donald G Welsh, Steven D Brooks, Paul D Chantler, Jefferson C Frisbee
PURPOSE: Although studies suggest elevated adrenergic activity paralleling metabolic syndrome in obese Zucker rats (OZR), the moderate hypertension and modest impact on organ perfusion questions the multi-scale validity of these data. METHODS: To understand how adrenergic function contributes to vascular reactivity in OZR, we utilized a multi-scale approach to investigate pressure responses, skeletal muscle blood flow and vascular reactivity following adrenergic challenge...
December 30, 2016: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/28030955/reno-cerebral-reflex-activates-renin-angiotensin-system-promoting-oxidative-stress-and-renal-damage-after-ischemia-reperfusion-injury
#10
Wei Cao, Aiqing Li, Jiawen Li, Chunyi Wu, Shuang Cui, Zhanmei Zhou, Youhua Liu, Christopher Stuart Wilcox, Fan Fan Hou
AIMS: A kidney-brain interaction has been described in acute kidney injury but the mechanisms are uncertain. Since we recently described a reno-cerebral reflex, we tested the hypothesis that renal ischemia-reperfusion injury activates a sympathetic reflex that interlinks the renal and cerebral renin-angiotensin axis to promote oxidative stress and progression of the injury. RESULTS: Bilateral ischemia-reperfusion activated the intrarenal and cerebral, but not the circulating, renin-angiotensin system, increased sympathetic activity in the kidney and the cerebral sympathetic regulatory regions, and induced brain inflammation and kidney injury...
December 28, 2016: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28006433/intracerebroventricular-tempol-administration-in-older-rats-reduces-oxidative-stress-in-the-hypothalamus-but-does-not-change-stat3-signalling-or-sirt1-ampk-pathway
#11
Hale Z Toklu, Philip J Scarpace, Yasemin Sakarya, Nataliya Kirichenko, Michael Matheny, Erin B Bruce, Christy S Carter, Drake Morgan, Nihal Tümer
Hypothalamic inflammation and increased oxidative stress are believed to be mechanisms that contribute to obesity. 4-Hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (tempol), a free radical scavenger, has been shown to reduce inflammation and oxidative stress. We hypothesized that brain infusion of tempol would reduce oxidative stress, and thus would reduce food intake and body weight and improve body composition in rats with age-related obesity and known elevated oxidative stress. Furthermore, we predicted an associated increase in markers of leptin signalling, including the silent mating type information regulator 2 homolog 1 (SIRT1)/5'AMP-activated protein kinase (AMPK) pathway and the signal transducer and activator of transcription 3 (STAT3) pathway...
January 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/27992529/exposure-of-bacterial-biofilms-to-electrical-current-leads-to-cell-death-mediated-in-part-by-reactive-oxygen-species
#12
Cassandra L Brinkman, Suzannah M Schmidt-Malan, Melissa J Karau, Kerryl Greenwood-Quaintance, Daniel J Hassett, Jayawant N Mandrekar, Robin Patel
Bacterial biofilms may form on indwelling medical devices such as prosthetic joints, heart valves and catheters, causing challenging-to-treat infections. We have previously described the 'electricidal effect', in which bacterial biofilms are decreased following exposure to direct electrical current. Herein, we sought to determine if the decreased bacterial quantities are due to detachment of biofilms or cell death and to investigate the role that reactive oxygen species (ROS) play in the observed effect. Using confocal and electron microscopy and flow cytometry, we found that direct current (DC) leads to cell death and changes in the architecture of biofilms formed by Gram-positive and Gram-negative bacteria...
2016: PloS One
https://www.readbyqxmd.com/read/27933463/effect-of-tempol-and-tempol-plus-catalase-on-intra-renal-haemodynamics-in-spontaneously-hypertensive-stroke-prone-shsp-and-wistar-rats
#13
Ahmad F Ahmeda, Mark G Rae, Mohammed F Al Otaibi, Lamyia M Anweigi, Edward J Johns
Vasoconstriction within the renal medulla contributes to the development of hypertension. This study investigated the role of reactive oxygen species (ROS) in regulating renal medullary and cortical blood perfusion (MBP and CBP respectively) in both stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar rats. CBP and MBP were measured using a laser-Doppler flow meter before and after intra-renal infusion of tempol, the superoxide dismutase (SOD) mimetic or tempol plus catalase, the hydrogen peroxide-degrading enzyme...
December 9, 2016: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/27907022/nicotine-induces-podocyte-apoptosis-through-increasing-oxidative-stress
#14
Xiqian Lan, Rivka Lederman, Judith M Eng, Seyedeh Shadafarin Marashi Shoshtari, Moin A Saleem, Ashwani Malhotra, Pravin C Singhal
BACKGROUND: Cigarette smoking plays an important role in the progression of chronic kidney disease (CKD). Nicotine, one of the major components of cigarette smoking, has been demonstrated to increase proliferation of renal mesangial cells. In this study, we examined the effect of nicotine on podocyte injury. METHODS: To determine the expression of nicotinic acetylcholine receptors (nAChR subunits) in podocytes, cDNAs and cell lysate of cultured human podocytes were used for the expression of nAChR mRNAs and proteins, respectively; and mouse renal cortical sections were subjected to immunofluorescant staining...
2016: PloS One
https://www.readbyqxmd.com/read/27899860/leptin-attenuates-the-contractile-function-of-adult-rat-cardiomyocytes-involved-in-oxidative-stress-and-autophagy
#15
Liu-Jin Luo, Ying-Ping Liu, Xun Yuan, Gui-Ping Zhang, Ning Hou, Xiao-Qian Wu, Jian-Dong Luo, Gen-Shui Zhang
BACKGROUND: Leptin has been identified as an important protein involved in obesity. As a chronic metabolic disorder, obesity is associated with a high risk of developing cardiovascular and metabolic diseases, including heart failure. The aim of this paper was to investigate the effects and the mechanism of leptin on the contractile function of cardiomyocytes in the adult rat. METHODS: Isolated adult rat cardiomyocytes were exposed to leptin (1, 10, and 100 nmol/L) for 1 hour...
November 2016: Acta Cardiologica Sinica
https://www.readbyqxmd.com/read/27890678/hot-spot-mapping-of-protein-surfaces-with-tempol-bovine-pancreatic-rnase-a-as-a-model-system
#16
Neri Niccolai, Edoardo Morandi, Simone Gardini, Valentino Costabile, Roberta Spadaccini, Orlando Crescenzi, Delia Picone, Ottavia Spiga, Andrea Bernini
TEMPOL spin-label has been used to identify surface exposure of protein nuclei from NMR analysis of the induced paramagnetic relaxation enhancements (PRE). The absence of linear dependence between atom depths and observed PRE reveals that specific mechanisms drive the approach of the paramagnet to the protein surface. RNase A represents a unique protein system to explore the fine details of the information offered by TEMPOL induced PRE, due to the abundance of previous results, obtained in solution and in the crystal, dealing with surface dynamics behavior of this protein...
February 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27887776/critical-role-of-superoxide-anions-and-hydroxyl-radicals-in-hema-induced-apoptosis
#17
H Schweikl, M Godula, C Petzel, C Bolay, K A Hiller, W Buchalla
OBJECTIVE: Resin monomers such as 2-hydroxyethyl methacrylate (HEMA) induce apoptosis because of the excess formation of reactive oxygen species (ROS). The portion of ROS including superoxide anions, hydrogen peroxide or hydroxyl radicals in monomer-induced apoptosis is unknown. Here, the effectiveness of superoxide anions or hydroxyl radicals was analyzed using tempol or sodium formate as radical scavengers. METHODS: RAW264.7 mouse macrophages were exposed to HEMA (0-6-8mM) in the presence of tempol (0-0...
November 22, 2016: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/27869744/protective-effect-of-tempol-against-cisplatin-induced-ototoxicity
#18
Cha Kyung Youn, Jun Kim, Eu-Ri Jo, Jeonghyun Oh, Nam Yong Do, Sung Il Cho
One of the major adverse effects of cisplatin chemotherapy is hearing loss. Cisplatin-induced ototoxicity hampers treatment because it often necessitates dose reduction, which decreases cisplatin efficacy. This study was performed to investigate the effect of Tempol on cisplatin-induced ototoxicity in an auditory cell line, House Ear Institute-Organ of Corti 1 (HEI-OC1). Cultured HEI-OC1 cells were exposed to 30 μM cisplatin for 24 h with or without a 2 h pre-treatment with Tempol. Cell viability was determined using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and apoptotic cells were identified using terminal deoxynucleotidyl transferase dUTP nick end labeling of nuclei (TUNEL) assay and flow cytometry...
November 18, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27866076/antioxidant-properties-of-tea-blunt-ros-dependent-lipogenesis-beneficial-effect-on-hepatic-steatosis-in-a-high-fat-high-sucrose-diet-nafld-obese-rat-model
#19
Laura Braud, Sylvain Battault, Grégory Meyer, Alessandro Nascimento, Sandrine Gaillard, Georges de Sousa, Roger Rahmani, Catherine Riva, Martine Armand, Jean-Michel Maixent, Cyril Reboul
Oxidative stress could trigger lipid accumulation in liver and thus hepatic steatosis. Tea is able to prevent liver disorders, but a direct link between antioxidant capacities and prevention of steatosis has not been reported yet. We aimed to investigate such relationship in a rat model of high fat-high sucrose diet (HFS)-induced obesity and to explore more deeply the mechanisms in isolated hepatocytes. Wistar rats were divided into a control group (standard diet), an HFS group (high fat-sucrose diet) and an HFS+tea group (HFS diet with ad-libitum access to tea drink)...
October 29, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/27861245/selective-impairment-of-blood-pressure-reduction-by-endothelial-nitric-oxide-synthase-dimer-destabilization-in-mice
#20
Tatsiana Suvorava, Stephanie Pick, Georg Kojda
OBJECTIVES: Endothelial dysfunction and oxidative stress are associated with hypertension but whether endothelial superoxide may play a role in the early development of essential hypertension remains uncertain. We investigated whether endothelial nitric oxide synthase (eNOS)-derived endothelial oxidative stress is involved in the regulation of SBP. METHODS: Wild-type eNOS [mice with endothelium-specific overexpression of bovine endothelial NO-synthase (eNOS-Tg)] or a novel dimer-destabilized eNOS-mutant harboring a partially disrupted zinc-finger [mice with endothelium-specific overexpression of destabilized bovine eNOS destabilized by replacement of Cys 101 to Ala (C101A-eNOS-Tg)] was introduced in C57BL/6 in an endothelial-specific manner...
January 2017: Journal of Hypertension
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