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https://www.readbyqxmd.com/read/28521553/glutaraldehyde-polymerized-hemoglobin-and-tempol-polyhb-tempol-has-superoxide-dismutase-activity-that-can-attenuate-oxidative-stress-on-endothelial-cells-induced-by-superoxide-anion
#1
Mengdi Wu, Kun Feng, Qiuhui Li, Huiya Ma, Hongli Zhu, Yudou Xie, Gaofei Yan, Chao Chen, Kunping Yan
A Tempol compound with an amine group (4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl, NH2-Tempol) was cross-linked to hemoglobin in a one-step polymerization reaction to produce a novel hemoglobin-based oxygen carrier (HBOC) designated PolyHb-Tempol. The reaction parameters, including the reaction time, pH, temperature, and ratio of reactants, were optimized, and the physiochemical properties of the resulting product were characterized. PolyHb-Tempol didn't show any toxicity towards endothelial cells. Furthermore, from observations of cell morphology and viability, PolyHb-Tempol showed a significant ability to inhibit or eliminate oxidative stress induced by superoxide free radicals...
May 18, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28515175/role-for-reactive-oxygen-species-in-flow-stimulated-inner-medullary-collecting-duct-endothelin-1-production
#2
Will Wheatley, Donald E Kohan
Inner medullary collecting duct (IMCD)-derived endothelin-1 (ET-1) is stimulated by volume expansion, in part through augmented luminal flow, whereupon it can elicit natriuresis and diuresis. Since flow can alter nitric oxide (NO) and reactive oxygen species (ROS), both of which can affect collecting duct salt transport, we asked whether NO and/or ROS mediate flow-stimulated IMCD ET-1. Mouse IMCD3 cells were exposed to flow and ET-1/GAPDH mRNA assessed. A shear stress of 10 dyne/cm2 for 1 hr increased ET-1 mRNA by 4-fold compared to no flow (ET-1 flow response)...
May 17, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28480509/oxidized-low-density-lipoprotein-stimulates-epithelial-sodium-channels-in-endothelial-cells-of-mouse-thoracic-aorta
#3
Chen Liang, Qiu-Shi Wang, Xu Yang, Na Niu, Qing-Qing Hu, Bao-Long Zhang, Ming-Ming Wu, Chang-Jiang Yu, Xiao Chen, Bin-Lin Song, Zhi-Ren Zhang, He-Ping Ma
BACKGROUND AND PURPOSE: Epithelial sodium channel (ENaC) is expressed in endothelial cells and acts as a negative modulator of vasodilation. Oxidized low-density lipoprotein (ox-LDL) is a key pathological factor in endothelial dysfunction. The present study is to examine the role of ENaC in ox-LDL-induced endothelial dysfunction and its associated signal transduction pathway. EXPERIMENTAL APPROACH: Patch-clamp techniques combined with pharmacological approaches were used to examine ENaC activity in the endothelial cells of split-open mouse thoracic aorta...
May 8, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28467798/mechanism-of-oxidative-stress-p38mapk-sgk1-signaling-axis-in-experimental-autoimmune-encephalomyelitis-eae
#4
Liang Wang, Bin Li, Mo-Yuan Quan, Lin Li, Yuan Chen, Guo-Jun Tan, Jing Zhang, Xiao-Peng Liu, Li Guo
BACKGROUND: Multiple sclerosis (MS), a complex disease associated with multifocal demyelination of the central nervous system and poorly understood etiology. It has been previously indicated that many factors, including oxidative stress and p38MAPK-SGK1 pathway, contribute to the pathogenesis of MS. METHODS: This study, using an experimental autoimmune encephalomyelitis (EAE) model system, was aimed at investigating the molecular mechanisms determining interaction p38MAPK-SGK1 pathway and oxidative stress in MS pathogenesis...
April 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28459156/antioxidant-tempol-supresses-heart-cytosolic-phospholipase-a-sub-2-sub-%C3%AE-stimulated-by-chronic-intermittent-hypoxia
#5
Petra Micova, Martina Klevstig, Kristyna Holzerova, Marek Vecka, Jitka Zurmanova, Jan Neckar, Frantisek Kolar, Olga Novakova, Jiri Novotny, Marketa Hlavackova
Adaptation to chronic intermittent hypoxia (CIH) is associated with reactive oxygen species (ROS) generation implicated in the improved cardiac tolerance against acute ischemia/reperfusion injury. Phospholipases A<sub>2</sub> (PLA<sub>2</sub>s) play an important role in cardiomyocyte phospholipid metabolism influencing membrane homeostasis. Here we aimed to determine the effect of CIH (7000 m, 8h/day, 5 weeks) on the expression of cytosolic PLA<sub>2</sub> (cPLA<sub>2</sub>α), its phosphorylated form (p-cPLA<sub>2</sub>α), calcium-independent (iPLA<sub>2</sub>) and secretory (sPLA<sub>2</sub>IIA) at protein and mRNA levels as well fatty acids (FA) profile in left ventricular myocardium of adult male Wistar rats...
April 30, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28456572/treatment-with-sodium-nitroprusside-improves-the-endothelial-function-in-aortic-rings-with-endothelial-dysfunction
#6
Tereza Cristina Buzinari, Jorge Camargo Oishi, Thiago Francisco De Moraes, Izabela Pereira Vatanabe, Heloisa Sobreiro Selistre-de-Araújo, Cezar Rangel Pestana, Gerson Jhonatan Rodrigues
PURPOSE: Verify if sodium nitroprusside (SNP) is able to improve endothelial function and if this effect is independent of nitric oxide (NO) release of the compound. METHODS: Normotensive (2K) and hypertensive (2K-1C) wistar rats were used. Intact endothelium aortas were placed in a myograph and incubated with SNP: 0.1nM; 1nM or 10nM during 30min. Cumulative concentration-effect curves for acetylcholine (Ach) were realized to measure the relaxing capacity. Intracellular NO were measured (by DAF-2DA probe) in HUVEC treated with SNP 0...
April 27, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28438648/the-superoxide-dismutase-mimetic-tempol-blunts-diabetes-induced-upregulation-of-nadph-oxidase-and-endoplasmic-reticulum-stress-in-a-rat-model-of-diabetic-nephropathy
#7
Miles J De Blasio, Anand Ramalingam, Anh H Cao, Darnel Prakoso, Ji-Ming Ye, Raelene Pickering, Anna M D Watson, Judy B de Haan, David M Kaye, Rebecca H Ritchie
Endoplasmic reticulum (ER) stress contributes to progression of diabetic nephropathy, which promotes end-stage renal failure in diabetic patients. This study was undertaken to investigate the actions of tempol and ramipril, pharmacological agents that target the consequences of NADPH oxidase, on diabetic nephropathy in a rat model of type 1 diabetes, with an emphasis on markers of ER stress. Male Sprague-Dawley rats were injected intravenously with a single bolus of streptozotocin (55mg/kg) to induce type 1 diabetes...
April 22, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28428533/protective-effect-of-tempol-against-hypoxia-induced-oxidative-stress-and-apoptosis-in-h9c2-cells
#8
Linlin Jing, Qian Li, Lei He, Wei Sun, Zhengping Jia, Huiping Ma
Background Material and Methods Results Conclusions.
April 21, 2017: Medical Science Monitor Basic Research
https://www.readbyqxmd.com/read/28423286/protective-effects-of-tempol-in-an-experimental-ovarian-ischemia-reperfusion-injury-model-in-female-wistar-albino-rats
#9
Neslihan Pınar, Oya Soylu Karapınar, Oğuzhan Özcan, Esin Atik Doğan, Suphi Bayraktar
The aim of this study was to investigate the antioxidant effects of tempol on ovarian ischemia-reperfusion injury in rats. Forty female Wistar albino rats were randomly divided into five groups: Group I: sham, Group II: ischemia (I), Group III: ischemia-reperfusion (I/R), Group IV: ischemia-reperfusion (I/R) + Tempol 30 mg/kg i.p, Group V: ischemia-reperfusion + Tempol 50 mg/kg i.p. Oxidative Stress Index (OSI) was significantly higher in the ischemia group and the I/R group compared with the sham group. Catalase levels were significantly lower in the I/R group than in the I/R + Tempol 30 mg/kg i...
April 19, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28397867/oxidative-stress-causes-hypertension-and-activation-of-nuclear-factor-%C3%AE%C2%BAb-after-high-fructose-and-salt-treatments
#10
Waleska C Dornas, Leonardo M Cardoso, Maísa Silva, Natália L S Machado, Deoclécio A Chianca-Jr, Andréia C Alzamora, Wanderson G Lima, Vincent Lagente, Marcelo E Silva
There is evidence that diets rich in salt or simple sugars as fructose are associated with abnormalities in blood pressure regulation. However, the mechanisms underlying pathogenesis of salt- and fructose-induced kidney damage and/or consequent hypertension yet remain largely unexplored. Here, we tested the role of oxidative state as an essential factor along with high salt and fructose treatment in causing hypertension. Fischer male rats were supplemented with a high-fructose diet (20% in water) for 20 weeks and maintained on high-salt diet (8%) associate in the last 10 weeks...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28396118/superoxide-and-hydrogen-peroxide-counterregulate-myogenic-contractions-in-renal-afferent-arterioles-from-a-mouse-model-of-chronic-kidney-disease
#11
Lingli Li, En Yin Lai, Zaiming Luo, Glenn Solis, Kathy K Griendling, W Robert Taylor, Pedro A Jose, Anton Wellsten, William J Welch, Christopher S Wilcox
Myogenic contractions protect kidneys from barotrauma but are impaired in chronic kidney disease (CKD). Since myogenic contractions are enhanced by superoxide but impaired by hydrogen peroxide, we tested the hypothesis that they are counterregulated by superoxide and H2O2 from NOX2/p47phox and/or NOX4/POLDIP2 in CKD. Myogenic contraction in isolated perfused afferent arterioles from mice with surgical 5/6 nephrectomy or sham operations fed a 6% sodium chloride diet was measured directly while superoxide and H2O2 were measured by fluorescence microscopy...
April 7, 2017: Kidney International
https://www.readbyqxmd.com/read/28388365/protective-effects-of-telmisartan-and-tempol-on-lipopolysaccharide-induced-cognitive-impairment-neuro-inflammation-and-amyloidogenesis-possible-role-of-brain-derived-neurotrophic-factor
#12
Waleed Abd-Elsalam Khallaf, Basim Messiha, Amira Abo-Youssef, Nesrine Salah El Dine El Sayed
Angiotensin II has pro-inflammatory and pro-oxidant potentials. We investigated the possible protective effects of the angiotensin receptor blocker telmisartan, compared with the superoxide scavenger tempol, on lipopolysaccharide (LPS)-induced cognitive decline and amyloidogenesis. Briefly, mice were allocated into a normal control group, an LPS control group, a tempol treatment group, and two telmisartan treatment groups. A behavioral study was conducted followed by a biochemical study via assessment of brain levels of beta amyloid (Aβ) and brain-derived neurotropic factor (BDNF) as amyloidogenesis and neuroplasticity markers, tumor necrosis factor-alpha (TNF-α), nitric oxide end products (NOx), neuronal and inducible nitric oxide synthase (nNOS and iNOS) as inflammatory markers, and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione reduced (GSH) and nitrotyrosine (NT) as oxido-nitrosative stress markers...
April 7, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28378130/the-role-of-actin-depolymerizing-factor-in-advanced-glycation-endproducts-induced-impairment-in-mouse-brain-microvascular-endothelial-cells
#13
Xinfeng Liu, Rongju Zhang, Hai Di, Dengfa Zhao, Jun Wang
Endothelial dysfunction is one of the most important pathological events during the development of several diabetic complications including stroke. The aim of this work was to investigate the role of actin depolymerizing factor (ADF) in advanced glycation endproducts (AGEs)-induced impairment in mouse brain microvascular endothelial cells (MBMECs). Incubation of MBMECs with AGEs led to downregulation of expression of ADF, which was reversed by treatment with soluble receptor of AGEs or tempol (an antioxidant)...
April 4, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28376643/effect-of-tempol-on-peripheral-neuropathy-in-diet-induced-obese-and-high-fat-fed-low-dose-streptozotocin-treated-c57bl6-j-mice
#14
Alexander Obrosov, Hanna Shevalye, Lawrence J Coppey, Mark A Yorek
In this study, we sought to determine the efficacy of tempol on multiple neuropathic endpoints in a diet-induced obese mouse, a model of pre-diabetes, and a high-fat fed low-dose streptozotocin treated mouse, a model of type 2 diabetes. Tempol (4-hydroxy-2,2,6,6-tetramethylpiperdine -1-oxyl) is a low molecular weight, water soluble, membrane permeable, and metal-independent superoxide dismutase mimetic that has been widely used in cellular studies for the removal of intracellular and extracellular superoxide...
April 19, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28376137/effects-of-tempol-and-quercetin-on-human-sperm-function-after-cryopreservation
#15
L Azadi, M Tavalaee, M R Deemeh, M Arbabian, M H Nasr-Esfahani
  BACKGROUND: Quercetin is a flavonoid with high reactive oxygen species (ROS) scavenging and ion chelating activity. It also enhances the activity of antioxidant enzymes and reduces enzymatic activity such as NADPH oxidase and NADH-dependent oxido-reductase. Tempol, as a superoxide dismutase mimetic agent, converts superoxide to less toxic hydrogen peroxide (H2O2), but cannot reduce highly toxic hydroxyl radicals in Fenton or Haber-Weiss reactions mediated with free iron or cupper. OBJECTIVE: The study was to compare the effect of Quercetin and Tempol in an optimized commercial cryo-protective media on ROS induced cryoinjury for the first time...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28374834/glycation-of-paraoxonase-1-by-high-glucose-instigates-endoplasmic-reticulum-stress-to-induce-endothelial-dysfunction-in-vivo
#16
Wei Yu, Xiaoli Liu, Liru Feng, Hui Yang, Weiye Yu, Tiejian Feng, Shuangxi Wang, Jun Wang, Ning Liu
High-density lipoprotein (HDL) modulates low-density lipoprotein and cell membrane oxidation through the action of paraoxonase-1 (PON1). Endoplasmic reticulum (ER) stress has been linked to a wide range of human pathologies including diabetes, obesity, and atherosclerosis. Previous studies have reported that PON1 is glycated in diabetes. The aim of this study is to investigate whether and how PON1 glycation contributes to endothelial dysfunction in diabetes. ER stress markers were monitored by western blot...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28366802/in-vivo-epr-pharmacokinetic-evaluation-of-the-redox-status-and-the-blood-brain-barrier-permeability-in-the-sod1-g93a-als-rat-model
#17
Stefan Stamenković, Aleksandra Pavićević, Miloš Mojović, Ana Popović-Bijelić, Vesna Selaković, Pavle Andjus, Goran Bačić
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder affecting the motor pathways of the central nervous system. Although a number of pathophysiological mechanisms have been described in the disease, post mortem and animal model studies indicate blood-brain barrier (BBB) disruption and elevated production of reactive oxygen species as major contributors to disease pathology. In this study, the BBB permeability and the brain tissue redox status of the SOD1(G93A) ALS rat model in the presymptomatic (preALS) and symptomatic (ALS) stages of the disease were investigated by in vivo EPR spectroscopy using three aminoxyl radicals with different cell membrane and BBB permeabilities, Tempol, 3-carbamoyl proxyl (3CP), and 3-carboxy proxyl (3CxP)...
March 31, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28344126/regulation-of-the-nitric-oxide-oxidase-activity-of-myeloperoxidase-by-pharmacological-agents
#18
Sophie L Maiocchi, Jonathan C Morris, Martin D Rees, Shane R Thomas
The leukocyte-derived heme enzyme myeloperoxidase (MPO) is released extracellularly during inflammation and impairs nitric oxide (NO) bioavailability by directly oxidizing NO or producing NO-consuming substrate radicals. Here, structurally diverse pharmacological agents with activities as MPO substrates/inhibitors or antioxidants were screened for their effects on MPO NO oxidase activity in human plasma and physiological model systems containing endogenous MPO substrates/antioxidants (tyrosine, urate, ascorbate)...
March 24, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28339958/postconditioning-protects-renal-fibrosis-by-attenuating-oxidative-stress-induced-mitochondrial-injury
#19
Shuxian Zhang, Xiaohua Tan, Yan Chen, Xiuying Zhang
Background.: Epithelial-mesenchymal transition (EMT) plays a critical role in renal fibrosis. We hypothesize that mitochondrial DNA damage and DNA deletions caused by reactive oxygen species (ROS) during renal ischemia-reperfusion injury (IRI) might lead to EMT in renal fibrosis. Methods.: Rats were classified into seven groups: sham-operation, IRI, postconditioning (POC), I/R + apocynin, POC +  apocynin, I/R + Mito-Tempol (Mito-T) and POC +  Mito-T...
March 3, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28337250/podocyte-injury-and-albuminuria-in-experimental-hyperuricemic-model-rats
#20
Shinichiro Asakawa, Shigeru Shibata, Chikayuki Morimoto, Takeshi Shiraishi, Takashi Nakamura, Yoshifuru Tamura, Takanori Kumagai, Makoto Hosoyamada, Shunya Uchida
Although hyperuricemia is shown to accelerate chronic kidney disease, the mechanisms remain unclear. Accumulating studies also indicate that uric acid has both pro- and antioxidant properties. We postulated that hyperuricemia impairs the function of glomerular podocytes, resulting in albuminuria. Hyperuricemic model was induced by oral administration of 2% oxonic acid, a uricase inhibitor. Oxonic acid caused a twofold increase in serum uric acid levels at 8 weeks when compared to control animals. Hyperuricemia in this model was associated with the increase in blood pressure and the wall-thickening of afferent arterioles as well as arcuate arteries...
2017: Oxidative Medicine and Cellular Longevity
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