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https://www.readbyqxmd.com/read/29677366/the-absence-of-indoleamine-2-3-dioxygenase-inhibits-retinal-capillary-degeneration-in-diabetic-mice
#1
Rooban B Nahomi, Sruthi Sampathkumar, Angela M Myers, Lynda Elghazi, Dawn G Smith, Jie Tang, C Allen Lee, Timothy S Kern, Ram H Nagaraj, Patrice E Fort
Purpose: Loss of retinal capillary endothelial cells and pericytes through apoptosis is an early event in diabetic retinopathy (DR). Inflammatory pathways play a role in early DR, yet the biochemical mechanisms are poorly understood. In this study, we investigated the role of indoleamine 2,3-dioxygenase (IDO), an inflammatory cytokine-inducible enzyme, on retinal endothelial apoptosis and capillary degeneration in the diabetic retina. Methods: IDO was detected in human and mouse retinas by immunohistochemistry or Western blotting...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29675997/hearing-vulnerability-after-noise-exposure-in-a-mouse-model-of-reactive-oxygen-species-overproduction
#2
Shigefumi Morioka, Hirofumi Sakaguchi, Taro Yamaguchi, Yuzuru Ninoyu, Hiroaki Mohri, Takashi Nakamura, Yasuo Hisa, Kiyokazu Ogita, Naoaki Saito, Takehiko Ueyama
Previous studies have convincingly argued that reactive oxygen species (ROS) contribute to the development of several major types of sensorineural hearing loss, such as noise-induced hearing loss (NIHL), drug-induced hearing loss, and age-related hearing loss. However, the underlying molecular mechanisms induced by ROS in these pathologies remain unclear. To resolve this issue, we established an in vivo model of ROS overproduction by generating a transgenic (TG) mouse line expressing the human NADPH oxidase 4 (NOX4, NOX4-TG mice), which is a constitutively active ROS-producing enzyme that does not require stimulation or an activator...
April 20, 2018: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29664131/anti-fibrotic-actions-of-ghrelin-by-inhibition-of-the-nadph-oxidase-ros-signaling-pathway
#3
Qian Wang, Xin Sui, Rui Chen, Peiyong Ma, Tao Ding, Dianjun Sui, Ping Yang
This study aims to investigate whether the molecular mechanisms by which Ghrelin inhibits cardiac fibrosis are linked to the NADPH oxidase-ROS signaling pathway. Adult male Sprague Dawley (SD) rats were randomly divided into 4 groups: sham, myocardial infarction (MI), MI + Ghrelin (100 μg/kg, twice daily, subcutaneous for 4 weeks beginning 24 h post-MI), or Ghrelin alone. Adult rat cardiac fibroblasts (CFs) were isolated and pretreated with or without Ghrelin for 24 h, followed by stimulation with or without angiotensin II (Ang II) for another 24 h...
April 17, 2018: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29627571/arginase-overexpression-and-nadph-oxidase-stimulation-underlie-impaired-vasodilation-induced-by-advanced-glycation-end-products
#4
Hany M El-Bassossy, Thikryat Neamatallah, Khadijah S Balamash, Amani T Abushareb, Malcolm L Watson
BACKGROUND: Advanced glycation endproducts (AGEs) play a major role in the development of many vascular complications that are mediated by endothelial dysfunction. The present work aimed to investigate the mechanism by which AGEs impair vasodilation. METHODS: The effect of AGEs on vasodilation induced by acetylcholine or D NONOate was examined by incubating isolated rat aortae with different AGEs concentrations. ACh-induced nitric oxide generation was assessed using the fluorescent probe diaminofluorecein (DAF-FM)...
April 5, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29619879/cisplatin-induced-oxidative-stress-stimulates-renal-fas-ligand-shedding
#5
Hitesh Soni, Damian Kaminski, Rajashekhar Gangaraju, Adebowale Adebiyi
Acute kidney injury (AKI), a significant complication of cisplatin chemotherapy is associated with reactive oxygen species (ROS)-dependent renal cell death, but the cellular targets of ROS in cisplatin nephrotoxicity are not fully resolved. Here, we investigated cisplatin-induced oxidative renal damage and tested the hypothesis that ROS-dependent shedding of death activator Fas ligand (FasL) occurs in cisplatin nephropathy. We show that intraperitoneal injection of sulfobutyl ether-β-cyclodextrin (Captisol™)-solubilized cisplatin elevated the level of lipid peroxidation product malondialdehyde in mouse kidneys and urinary concentration of oxidative DNA damage biomarker 8-hydroxy-2'-deoxyguanosine...
November 2018: Renal Failure
https://www.readbyqxmd.com/read/29615926/circadian-differences-in-the-contribution-of-the-brain-renin-angiotensin-system-in-genetically-hypertensive-mice
#6
Kristy L Jackson, Francine Z Marques, Kyungjoon Lim, Pamela J Davern, Geoffrey A Head
Objective: Genetically hypertensive BPH/2J mice are recognized as a neurogenic model of hypertension, primarily based on sympathetic overactivity and greater neuronal activity in cardiovascular regulatory brain regions. Greater activity of the central renin angiotensin system (RAS) and reactive oxygen species (ROS) reportedly contribute to other models of hypertension. Importantly the peripheral RAS contributes to the hypertension in BPH/2J mice, predominantly during the dark period of the 24 h light cycle...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29614490/in-utero-exposure-to-fine-particulate-matter-causes-hypertension-due-to-impaired-renal-dopamine-d1-receptor-in-offspring
#7
Zhengmeng Ye, Xi Lu, Yi Deng, Xinquan Wang, Shuo Zheng, Hongmei Ren, Miao Zhang, Tingting Chen, Pedro A Jose, Jian Yang, Chunyu Zeng
BACKGROUND/AIMS: Adverse environment in utero can modulate adult phenotypes including blood pressure. Fine particulate matter (PM2.5) exposure in utero causes hypertension in the offspring, but the exact mechanisms are not clear. Renal dopamine D1 receptor (D1R), regulated by G protein-coupled receptor kinase type 4 (GRK4), plays an important role in the regulation of renal sodium transport and blood pressure. In this present study, we determined if renal D1R dysfunction is involved in PM2...
March 21, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29604595/ameliorating-effects-of-tempol-on-methotrexate-induced-liver-injury-in-rats
#8
Neslihan Pınar, Mahir Kaplan, Tümay Özgür, Oğuzhan Özcan
Methotrexate (MTX) is used in the treatment of certain types of cancers and chronic inflammatory illnesses, although the clinical use of MTX is limited due to its adverse effects, the most common of which are hepatotoxicity and nephrotoxicity. In the present study, we demonstrate the protecting influence of tempol related to oxidative stress in MTX-induced liver toxicity in rats using histopathological and biochemical parameters. The rats were divided into four groups: control group (group 1), tempol group (group 2), MTX group (group 3) and MTX + tempol group (group 4)...
March 28, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29589623/nanoparticle-cell-interactions-induced-apoptosis-a-case-study-with-nanoconjugated-epidermal-growth-factor
#9
Ali Khanehzar, Juan C Fraire, Min Xi, Amin Feizpour, Fangda Xu, Linxi Wu, Eduardo A Coronado, Björn M Reinhard
In addition to the intrinsic toxicity associated with the chemical composition of nanoparticles (NP) and their ligands, biofunctionalized NP can perturb specific cellular processes through NP-cell interactions and induce programmed cell death (apoptosis). In the case of the epidermal growth factor (EGF), nanoconjugation has been shown to enhance the apoptotic efficacy of the ligand, but the critical aspects of the underlying mechanism and its dependence on the NP morphology remain unclear. In this manuscript we characterize the apoptotic efficacy of nanoconjugated EGF as a function of NP size (with sphere diameters in the range 20-80 nm), aspect ratio (A...
March 28, 2018: Nanoscale
https://www.readbyqxmd.com/read/29574160/reactive-oxygen-species-participate-in-liver-function-recovery-during-compensatory-growth-in-zebrafish-danio-rerio
#10
Jirong Jia, Yazhou Zhang, Xi Yuan, Jingkai Qin, Guokun Yang, Xiaozheng Yu, Bin Wang, Caiyun Sun, Wensheng Li
Compensatory growth (CG) is defined as a phase of accelerated growth when the disadvantageous environment is improved, accompanied by metabolic adjustment. Here, we report that hepatic oxidative phosphorylation (OXPHOS) activity was enhanced during compensatory growth in zebrafish. Mitochondrial metabolism enabled the generation of reactive oxygen species (ROS), which activated the nrf2 (nuclear factor-erythroid 2-related factor 2) signaling pathway, as well as the mTOR signaling pathway. Tempol (a superoxide dismutase mimetic) treatment blocked ROS signaling in the liver as well as CG in zebrafish...
March 23, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29518609/suppression-of-nrf2-confers-chemosensitizing-effect-through-enhanced-oxidant-mediated-mitochondrial-dysfunction
#11
Vachirapong Sompakdee, Auemduan Prawan, Laddawan Senggunprai, Upa Kukongviriyapan, Papavee Samathiwat, Jaroon Wandee, Veerapol Kukongviriyapan
AIMS: Transcription factor Nrf2, which regulates the expression of cytoprotective and antioxidant enzymes, contributes to proliferation and resistance to chemotherapy in cancer. The inhibition of Nrf2 can sensitize cholangiocarcinoma (CCA) cells to the cytotoxicity of several chemotherapeutic agents. In this study, we investigated the mechanism of this chemosensitizing effect. MAIN METHODS: KKU-100 cells were used in the study. Nrf2 expression was knocked down by siRNA and expression was validated by reverse transcription and polymerase chain reaction...
March 5, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29513566/reactive-oxygen-species-up-regulate-expression-of-muscle-atrophy-associated-ubiquitin-ligase-cbl-b-in-rat-l6-skeletal-muscle-cells
#12
Takayuki Uchida, Yoshihiro Sakashita, Kanako Kitahata, Yui Yamashita, Chisato Tomida, Yuki Kimori, Akio Komatsu, Katsuya Hirasaka, Ayako Ohno, Reiko Nakao, Atsushi Higashitani, Akira Higashibata, Noriaki Ishioka, Toru Shimazu, Takeshi Kobayashi, Yuushi Okumura, Inho Choi, Motoko Oarada, Edward M Mills, Shigetada Teshima-Kondo, Shin'ichi Takeda, Eiji Tanaka, Keiji Tanaka, Masahiro Sokabe, Takeshi Nikawa
Unloading-mediated muscle atrophy is associated with increased reactive oxygen species (ROS) production. We previously demonstrated that elevated ubiquitin ligase casitas B-lineage lymphoma-b (Cbl-b) resulted in the loss of muscle volume [Nakao R. et al. Mol Cell Biol, 29: 4798-4811, 2009]. However, the pathological role of ROS production associated with unloading-mediated muscle atrophy still remains unknown. Here, we showed the ROS-mediated signal transduction caused by microgravity or its simulation contributes to Cbl-b expression...
March 7, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29507270/antioxidative-effects-of-tempol-on-mitochondrial-dysfunction-in-diabetic-nephropathy
#13
Akram Ranjbar, Nejat Kheiripour, Hassan Ghasemi, Mohamad Ali Seif Rabiei, Farahnaz Dadras, Farhad Khoshjou
INTRODUCTION: Oxidative stress has a well-known role in diabetic nephropathy, and mitochondria are the major source of reactive oxygen species production. This study aimed to assess the effect of tempol, a superoxide dismutase mimetic agent, on mitochondrial antioxidant enzymes and cell viability in diabetic nephropathy. MATERIALS AND METHODS: Adult male Wistar rats were divided into 4 groups of 7 animals. Diabetes mellitus was induced by injection of streptozotocin in 2 groups, the rat in one of which were also treated with tempol for 4 weeks...
March 2018: Iranian Journal of Kidney Diseases
https://www.readbyqxmd.com/read/29493032/an-overhauser-enhanced-mri-platform-for-dynamic-free-radical-imaging-in-vivo
#14
David E J Waddington, Mathieu Sarracanie, Najat Salameh, Fanny Herisson, Cenk Ayata, Matthew S Rosen
Overhauser-enhanced MRI (OMRI) is an electron-proton double-resonance imaging technique of interest for its ability to non-invasively measure the concentration and distribution of free radicals. In vivo OMRI experiments are typically undertaken at ultra-low magnetic field (ULF), as both RF power absorption and penetration issues-a consequence of the high resonance frequencies of electron spins-are mitigated. However, working at ULF causes a drastic reduction in MRI sensitivity. Here, we report on the design, construction and performance of an OMRI platform optimized for high NMR sensitivity and low RF power absorbance, exploring challenges unique to probe design in the ULF regime...
March 1, 2018: NMR in Biomedicine
https://www.readbyqxmd.com/read/29480820/translational-repression-of-hif2%C3%AE-expression-in-mice-with-chuvash-polycythemia-reverses-polycythemia
#15
Manik C Ghosh, De-Liang Zhang, Hayden Ollivierre, Michael A Eckhaus, Tracey A Rouault
Chuvash polycythemia is an inherited disease caused by a homozygous germline VHLR200W mutation, which leads to impaired degradation of HIF2α, elevated levels of serum erythropoietin, and erythrocytosis/polycythemia. This phenotype is recapitulated by a mouse model bearing a homozygous VhlR200W mutation. We previously showed that iron-regulatory protein 1-knockout (Irp1-knockout) mice developed erythrocytosis/polycythemia through translational derepression of Hif2α, suggesting that IRP1 could be a therapeutic target to treat Chuvash polycythemia...
February 26, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29465305/glomerular-hyperpermeability-after-acute-unilateral-ureteral-obstruction-effects-of-tempol-nos-rhoa-and-rac-1-inhibition
#16
Julia Dolinina, Anna Rippe, Peter Bentzer, Carl M Öberg
It is well known that proteinuria following urinary tract obstruction is mainly of a tubular nature. Whether there are also changes in glomerular permeability is however unknown. In this study we compared glomerular sieving coefficients (θ) of polydisperse fluorescein isothiocyanate (FITC)-Ficoll 70/400 following 120 or 180 min unilateral ureteral obstruction (UUO) in anesthetized Sprague-Dawley rats. Samples were collected from the obstructed kidney at 5, 15 and 30 minutes post-release and analyzed by means of high pressure size-exclusion chromatography (HP-SEC)...
February 21, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29458041/role-of-superoxide-ion-formation-in-hypothermia-rewarming-induced-contractile-dysfunction-in-cardiomyocytes
#17
Niccole Schaible, Young Soo Han, Torkjel Tveita, Gary C Sieck
Rewarming following accidental hypothermia is associated with circulatory collapse due primarily to impaired cardiac contractile (systolic) function. Previously, we found that reduced myofilament Ca2+ sensitivity underlies hypothermia/rewarming (H/R)-induced cardiac contractile dysfunction. This reduced Ca2+ sensitivity is associated with troponin I (cTnI) phosphorylation. We hypothesize that H/R induces reactive oxygen species (ROS) formation in cardiomyocytes, which leads to cTnI phosphorylation and reduced myofilament Ca2+ sensitivity...
February 16, 2018: Cryobiology
https://www.readbyqxmd.com/read/29456512/local-injections-of-superoxide-dismutase-attenuate-the-exercise-pressor-reflex-in-rats-with-femoral-artery-occlusion
#18
Jihong Xing, Jian Lu, Jiahao Liu, Jianhua Li
The exercise pressor reflex is amplified in patients with peripheral artery disease (PAD) and in an experimental PAD model of rats induced by femoral artery occlusion. Heightened blood pressure worsens the restricted blood flow directed to the limbs in this disease. The purpose of this study was to determine the role played by muscle oxidative stress in regulating the augmented pressor response to static exercise in PAD. We hypothesized that limb ischemia impairs muscle superoxide dismutase (SOD) thereby leading to abnormal autonomic responsiveness observed in PAD animals, and a chronic compensation of SOD for anti-oxidation improves the exaggerated exercise pressor reflex...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29433520/intermittent-hypoxia-promotes-melanoma-lung-metastasis-via-oxidative-stress-and-inflammation-responses-in-a-mouse-model-of-obstructive-sleep-apnea
#19
Lian Li, Fangyuan Ren, Chao Qi, Leiqian Xu, Yinshan Fang, Maoli Liang, Jing Feng, Baoyuan Chen, Wen Ning, Jie Cao
BACKGROUND: Recently, increased tumor incidence and cancer-related mortality have been reported among patients with obstructive sleep apnea (OSA). Intermittent hypoxia (IH), the hallmark feature of OSA, contributes to the metastasis of tumors. However, the molecular mechanisms by which tumor metastasis is accelerated by OSA-like IH remain to be elucidated. METHODS: C57BL/6 J male mice were subjected to intravenous injection of B16F10 melanoma cells before receiving IH treatment...
February 12, 2018: Respiratory Research
https://www.readbyqxmd.com/read/29431894/sample-ripening-through-nanophase-separation-impacts-the-performance-of-dynamic-nuclear-polarization
#20
Emmanuelle Weber, Giuseppe Sicoli, Hervé Vezin, Ghislaine Frébourg, Daniel Abergel, Geoffrey Bodenhausen, Dennis Kurzbach
Mixtures of water and glycerol provide popular matrices for low-temperature spectroscopy of vitrified samples. However, they involve counterintuitive physicochemical properties, such as spontaneous nanoscopic phase separations (NPS) in solutions that appear macroscopically homogeneous. We demonstrate that such phenomena can substantially impact the efficiency of dynamic nuclear polarization (DNP) by factors up to 20% by causing fluctuations in local concentrations of polarization agents (radicals). Thus, a spontaneous NPS of water/glycerol mixtures that takes place on time scales on the order of 30-60 min results in a confinement of polarization agents in nanoscopic water-rich vesicles, which in return affects the DNP...
February 12, 2018: Angewandte Chemie
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