keyword
MENU ▼
Read by QxMD icon Read
search

Click chemistry

keyword
https://www.readbyqxmd.com/read/28944064/de-novo-synthesis-of-alkyne-substituted-tryptophans-as-chemical-probes-for-protein-profiling-studies
#1
R N Nair, J J Rosnow, T A Murphree, M E Bowden, S R Lindemann, A T Wright
De novo synthesis of alkynalted tryptophan moieties as chemical probes for protein profilling studies, via an unexpected synthesis of Michael acceptor 12 is reported. Friedel Craft's alkylation of 12 and alkyne substituted indoles gave alkynalated tryptophan moieties, which perform as chemical probe by incorporating into actively translating Escherichia coli cells, whereby the alkyne moiety enables multimodal analyses through click chemistry mediated attachment of reporting groups.
April 1, 2017: Organic Chemistry Frontiers: An International Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28944050/carbohydrates-as-potentially-versatile-core-subcarriers-for-multivalent-immunogens
#2
Divya Kushwaha, Peng Xu, Pavol Kováč
Synthetic multivalent glycoclusters that carry carbohydrate antigen epitopes have been recognized as promising candidates for the development of carbohydrate based vaccines. Here we describe a convergent strategy for the synthesis of conjugation-ready multivalent glycoclusters using sugars as versatile core subcarriers. d-Glucose and gentiobiose were converted into poly-alkyne functionalized cores which were then decorated with an azide bearing model ligand d-glucose using click chemistry, to form structurally well-defined tetra- and heptavalent glycoclusters...
2017: RSC Advances
https://www.readbyqxmd.com/read/28935952/application-of-bio-orthogonal-proteome-labeling-to-cell-transplantation-and-heterochronic-parabiosis
#3
Yan Liu, Michael J Conboy, Melod Mehdipour, Yutong Liu, Thanhtra P Tran, Aaron Blotnick, Prasanna Rajan, Thalie Cavalcante Santos, Irina M Conboy
Studies of heterochronic parabiosis demonstrated that with age, the composition of the circulatory milieu changes in ways that broadly inhibit tissue regenerative capacity. In addition, local tissue niches have age-specific influences on their resident stem cells. Here we use bio-orthogonal proteome labeling for detecting in vivo proteins present only in transplanted myoblasts, but not in host tissue, and proteins exclusive to one young mouse and transferred during parabiosis to its old partner. We use a transgenic mouse strain that ubiquitously expresses a modified tRNA methionine synthase, metRS, which preferentially incorporates the methionine surrogate azido-nor-leucine (ANL) into newly generated proteins...
September 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28935106/chemoenzymatic-glycan-remodeling-of-natural-and-recombinant-glycoproteins
#4
Qiang Yang, Lai-Xi Wang
N-glycosylation plays important roles in modulating the biological functions of glycoproteins, such as protein folding, stability, and immunogenicity. However, acquiring homogeneous glycoforms of glycoproteins has been a challenging task for functional studies and therapeutic applications. In this chapter, we describe an efficient chemoenzymatic glycan remodeling protocol for making homogeneous glycoproteins that involves enzymatic deglycosylation and subsequent reglycosylation procedures. Two therapeutic glycoproteins, Herceptin (trastuzumab, a therapeutic monoclonal antibody) and erythropoietin (EPO, a glycoprotein hormone), were chosen as the model systems...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28927630/sustainable-thermoplastic-elastomers-derived-from-cellulose-fatty-acid-and-furfural-via-atrp-and-click-chemistry
#5
Juan Yu, Chuanwei Lu, Chunpeng Wang, Jifu Wang, Yimin Fan, Fuxiang Chu
Cellulose-based thermoplastic elastomers (TPEs) have attracted considerable attention because of their rigid backbone, good mechanical properties, renewable nature and abundance. In the present study, sustainable TPEs based on ethyl cellulose (EC), fatty acid and furfural were generated by the combination of ATRP and "click chemistry". To fabricate sustainable TPEs with higher toughness, a range of polymers, including mono random-copolymer poly(tetrahydrofurfuryl methacrylate-co-lauryl methacrylate) (P(THFMA-co-LMA), dual polymer side chains PTHFMA and PLMA, and mono-block copolymer PTHFMA-b-PLMA, were designed as side chains to fabricate EC brush copolymers with random, dual or block side chain architectures using the "grafting from" and "grafting onto" methods...
November 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28926550/bioorthogonal-chemistry-click-on-click-off
#6
Caitlin Deane
No abstract text is available yet for this article.
September 19, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28921946/biomimetic-magnetosomes-as-versatile-artificial-antigen-presenting-cells-to-potentiate-t-cell-based-anticancer-therapy
#7
Qianmei Zhang, Wei Wei, Peilin Wang, Liping Zuo, Feng Li, Jin Xu, Xiaobo Xi, Xiaoyong Gao, Guanghui Ma, Hai-Yan Xie
Adoptive T-cell transfer for cancer therapy relies on both effective ex vivo T-cell expansion and in vivo targeting performance. One promising but challenging method for accomplishing this purpose is to construct multifunctional artificial antigen-presenting cells (aAPCs). We herein developed biomimetic magnetosomes as versatile aAPCs, wherein magnetic nanoclusters were coated with azide-engineered leucocyte membranes and then decorated with T-cell stimuli through copper-free click chemistry. These nano aAPCs not only exhibited high performance for antigen-specific cytotoxic T-cell (CTL) expansion and stimulation but also visually and effectively guided reinfused CTLs to tumor tissues through magnetic resonance imaging and magnetic control...
September 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/28921867/polymer-cancerostatics-targeted-with-an-antibody-fragment-bound-via-a-coiled-coil-motif-in-vivo-therapeutic-efficacy-against-murine-bcl1-leukemia
#8
Michal Pechar, Robert Pola, Olga Janoušková, Irena Sieglová, Vlastimil Král, Milan Fábry, Barbora Tomalová, Marek Kovář
A BCL1 leukemia-cell-targeted polymer-drug conjugate with a narrow molecular weight distribution consisting of an N-(2-hydroxypropyl)methacrylamide copolymer carrier and the anticancer drug pirarubicin is prepared by controlled radical copolymerization followed by metal-free click chemistry. A targeting recombinant single chain antibody fragment (scFv) derived from a B1 monoclonal antibody is attached noncovalently to the polymer carrier via a coiled coil interaction between two complementary peptides. Two pairs of coiled coil forming peptides (abbreviated KEK/EKE and KSK/ESE) are used as linkers between the polymer-pirarubicin conjugate and the targeting protein...
September 15, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28905618/functionalization-of-reduced-graphene-oxide-via-thiol-maleimide-click-chemistry-facile-fabrication-of-targeted-drug-delivery-vehicles
#9
Yavuz Oz, Alexandre Barras, Rana Sanyal, Rabah Boukherroub, Sabine Szunerits, Amitav Sanyal
Materials based on reduced graphene oxide (rGO) have shown to be amenable to noncovalent functionalization through hydrophobic interactions. The scaffold, however, does not provide sufficient covalent linkage given the low number of reactive carboxyl and alcohol groups typically available on the rGO. The integration of clickable groups, particularly the ones that can undergo efficient conjugation without any metal catalyst, would allow facile functionalization of these materials. This study reports on the noncovalent association of a maleimide-containing catechol (dopa-MAL) surface anchor onto the rGO...
September 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28904627/mechanochemical-synthesis-of-thioureas-ureas-and-guanidines
#10
REVIEW
Vjekoslav Štrukil
In this review, the recent progress in the synthesis of ureas, thioureas and guanidines by solid-state mechanochemical ball milling is highlighted. While the literature is abundant on their preparation in conventional solution environment, it was not until the advent of solvent-free manual grinding using a mortar and pestle and automated ball milling that new synthetic opportunities have opened. The mechanochemical approach not only has enabled the quantitative synthesis of (thio)ureas and guanidines without using bulk solvents and the generation of byproducts, but it has also been established as a means to develop "click-type" chemistry for these classes of compounds and the concept of small molecule desymmetrization...
2017: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28898061/post-synthetic-modification-of-bacterial-peptidoglycan-using-bioorthogonal-n-acetylcysteamine-analogs-and-peptidoglycan-o-acetyltransferase-b
#11
Yiben Wang, Klare M Lazor, Kristen Elizabeth DeMeester, Hai Liang, Tyler K Heiss, Catherine Leimkuhler Grimes
Bacteria have the natural ability to install protective post-synthetic modifications onto its bacterial peptidoglycan (PG), the coat woven into bacterial cell wall. Peptidoglycan O-acetyltransferase B (PatB) catalyzes the O-acetylation of PG in Gram (-) bacteria, which aids in bacterial survival, as it prevents autolysins such as lysozyme from cleaving the PG. We explored the mechanistic details of PatB's acetylation function and determined that PatB has substrate specificity for bioorthgonal short N-acetyl cysteamine (SNAc) donors...
September 12, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28894874/specific-and-spatial-labeling-of-choline-containing-teichoic-acids-in-streptococcus-pneumoniae-by-click-chemistry
#12
A M Di Guilmi, J Bonnet, S Peiβert, C Durmort, B Gallet, T Vernet, N Gisch, Y-S Wong
Propargyl-choline was efficiently incorporated into teichoic acid (TA) polymers on the surface of Streptococcus pneumoniae. The introduction of a fluorophore by click chemistry enabled sufficient labeling of the pneumococcus, as well as its specific detection when mixed with other bacterial species. The labeling is localized at the septal site, suggesting a similar location of the TA and peptidoglycan (PG) synthetic machineries. This method is a unique opportunity to improve our understanding of the spatial location of pneumococcal TA biosynthesis...
September 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28892360/in-vivo-imaging-guided-photothermal-photoacoustic-synergistic-therapy-with-bioorthogonal-metabolic-glycoengineering-activated-tumor-targeting-nanoparticles
#13
Lihua Du, Huan Qin, Teng Ma, Tao Zhang, Da Xing
Developing multifunctional phototheranostics with nanoplatforms offers promising potential for effective eradication of malignant solid tumors. In this study, we develop a multifunctional phototheranostic by combining photothermal therapy (PTT) and photoacoustic therapy (PAT) based on a tumor-targeting nanoagent (DBCO-ZnPc-LP). The nanoagent DBCO-ZnPc-LP was facilely prepared by self-assembling of a single lipophilic near-infrared (NIR) dye zinc(II)-phthalocyanine (ZnPc) with a lipid-poly(ethylene glycol) (LP) and following modified further with dibenzyl cyclootyne (DBCO) for introducing the two-step chemical tumor-targeting strategy based on metabolic glycoengineering and click chemistry...
September 13, 2017: ACS Nano
https://www.readbyqxmd.com/read/28891636/cuaac-based-click-chemistry-in-self-healing-polymers
#14
Diana Döhler, Philipp Michael, Wolfgang H Binder
Click chemistry has emerged as a significant tool for materials science, organic chemistry, and bioscience. Based on the initial concept of Barry Sharpless in 2001, the copper(I)-catalyzed azide/alkyne cycloaddition (CuAAC) reaction has triggered a plethora of chemical concepts for linking molecules and building blocks under ambient conditions, forming the basis for applications in autonomous cross-linking materials. Self-healing systems on the other hand are often based on mild cross-linking chemistries that are able to react either autonomously or upon an external trigger...
September 11, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28888965/-click-reaction-based-synthesis-of-nimbolide-derivatives-and-study-of-their-insect-antifeedant-activity-against-spodoptera-litura-larvae
#15
Bandi Siva, Amujuri Devi, Arramsetti Venkanna, Borra Poornima, Genji Sukumar, Solipeta Divya Reddy, Movva Vijaya, Ramesh Ummanni, Katragadda Suresh Babu
A series of Nimbolide-triazole conjugates were synthesized through copper(I)- catalyzed azide-alkyne "click" chemistry approach and these derivatives (2-4, 2a-2l) were characterized using modern spectroscopic techniques. Antifeedant activities of these derivatives were studied on Tobacco Caterpillar, Spodoptera litura (F.) using no-choice leaf disk bioassay. Interestingly, the synthesized derivatives were more effective in reducing feedancy by insect species when compared to the parent nimbolide. Among the tested compounds, 2a, 2c, and 2d showed potent antifeedancy with ED50 values of 0...
September 6, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28884571/clickable-multifunctional-dumbbell-particles-for-in-situ-multiplex-single-cell-cytokine-detection
#16
Peng Zhao, Justin George, Bin Li, Nooshin Amini, Janet Paluh, Jun Wang
We report a novel strategy for fabrication of multifunctional dumbbell particles (DPs) through click chemistry for monitoring single-cell cytokine releasing. Two different types of DPs were prepared on a large scale through covalent bioorthogonal reaction between methyltetrazine and trans-cyclooctene on a microchip under a magnetic field. After collection of the DPs, the two sides of each particle were further functionalized with different antibodies for cell capturing and cytokine detection, respectively. These DPs labeled with different fluorescent dyes have been used for multiplex detection and analysis of cytokines secreted by single live cells...
September 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28881435/versatile-method-for-the-site-specific-modification-of-dna-with-boron-clusters-anti-egfr-antisense-oligonucleotide-case
#17
Zbigniew Jan Lesnikowski, Katarzyna Ebenryter-Olbińska, Damian Kaniowski, Milena Sobczak, Blazej Wojtczak, Slawomir Janczak, Ewelina Wielgus, Barbara Nawrot
A general and convenient approach for the incorporation of different types of boron clusters into specific locations of the DNA-oligonucleotide chain based on automated phosphoramidite method of oligonucleotide synthesis and post synthetic "click chemistry" modification was developed. Pronounced effects of boron cluster modification on the physicochemical and biochemical properties of the antisense oligonucleotides were observed. The silencing activity of antisense oligonucleotides bearing a single boron cluster modification in the middle of the oligonucleotide chain was substantially higher than that of unmodified oligonucleotides...
September 7, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28871452/click-chemistry-mediated-cyclic-cleavage-of-metal-ion-dependent-dnazymes-for-amplified-and-colorimetric-detection-of-human-serum-copper-ii
#18
Daxiu Li, Jiaqing Xie, Wenjiao Zhou, Bingying Jiang, Ruo Yuan, Yun Xiang
The determination of the level of Cu(2+) plays important roles in disease diagnosis and environmental monitoring. By coupling Cu(+)-catalyzed click chemistry and metal ion-dependent DNAzyme cyclic amplification, we have developed a convenient and sensitive colorimetric sensing method for the detection of Cu(2+) in human serums. The target Cu(2+) can be reduced by ascorbate to form Cu(+), which catalyzes the azide-alkyne cycloaddition between the azide- and alkyne-modified DNAs to form Mg(2+)-dependent DNAzymes...
September 4, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28863308/clickable-and-imageable-multiblock-polymer-micelles-with-magnetically-guided-and-peg-switched-targeting-and-release-property-for-precise-tumor-theranosis
#19
Jing Wei, Xiaoyu Shuai, Rui Wang, Xueling He, Yiwen Li, Mingming Ding, Jiehua Li, Hong Tan, Qiang Fu
Targeted delivery of therapeutics and diagnostics using nanotechnology holds great promise to minimize the side effects of conventional chemotherapy and enable specific and real-time detection of diseases. To realize this goal, we report a clickable and imageable nanovehicle assembled from multiblock polyurethanes (MPUs). The soft segments of the polymers are based on detachable poly(ethylene glycol) (PEG) and degradable poly(ε-caprolactone) (PCL), and the hard segments are constructed from lysine- and cystine-derivatives bearing reduction-responsive disulfide linkages and click-active alkynyl moieties, allowing for post-conjugation of targeting ligands via a click chemistry...
August 18, 2017: Biomaterials
https://www.readbyqxmd.com/read/28863243/one-pot-multicomponent-synthesis-of-glycopolymers-through-a-combination-of-host-guest-interaction-thiol-ene-and-copper-catalyzed-click-reaction-in-water
#20
Xiang Hu, Jinbo Gao, Yan Luo, Ting Wei, Yishi Dong, Gaojian Chen, Hong Chen
There is a common phenomenon that the heterogeneity of natural oligosaccharides contains various sugar units, which can be used to enhance affinity and selectivity toward a specific receptor, so the synthesis of heterogeneous glycopolymers is always an important issue in the glycopolymer field. Herein, this study conducts a one-pot method to prepare polyrotaxane-based heteroglycopolymers anchored with different sugar units and fluorescent moieties via the combination of host-guest interaction, thiol-ene, and copper-catalyzed click chemistry in water...
September 1, 2017: Macromolecular Rapid Communications
keyword
keyword
95886
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"