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https://www.readbyqxmd.com/read/28735640/dose-equivalents-for-second-generation-long-acting-injectable-antipsychotics-the-minimum-effective-dose-method
#1
REVIEW
Philipp H Rothe, Stephan Heres, Stefan Leucht
BACKGROUND: The concept of dose equivalence of depot medication is important for many scientific and clinical purposes. METHODS: A systematic literature search on four second-generation antipsychotics available as long-acting injectable drugs and haloperidol was conducted. We used the minimum effective dose method which is based on randomized fixed dose studies where the smallest dose which was significantly more efficacious than placebo in the primary outcome was declared as minimum effective dose...
July 20, 2017: Schizophrenia Research
https://www.readbyqxmd.com/read/28733465/the-retinol-binding-protein-receptor-stra6-regulates-diurnal-insulin-responses
#2
Christy M Gliniak, J Mark Brown, Noa Noy
It has long been appreciated that insulin action is closely tied to circadian rhythms. However, mechanisms that dictate diurnal insulin sensitivity in metabolic tissues are not well understood. Retinol binding protein 4 (RBP4) has been implicated as a driver of insulin resistance in rodents and humans, and has become an attractive drug target in type 2 diabetes. RBP4 is synthesized primarily in the liver where it binds retinol and transports it to tissues throughout the body. The retinol-RBP4 complex (holo-RBP) can be recognized by a cell surface receptor known as stimulated by retinoic acid 6 (STRA6) which transports retinol into cells...
July 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28733251/rna-binding-protein-raly-promotes-protein-arginine-methyltransferase-1-alternatively-spliced-isoform-v2-relative-expression-and-metastatic-potential-in-breast-cancer-cells
#3
Emma Bondy-Chorney, R Mitchell Baldwin, Andréanne Didillon, Benoît Chabot, Bernard J Jasmin, Jocelyn Côté
Aberrant expression of Protein Arginine Methyltransferases (PRMTs) has been observed in several cancer types, including breast cancer. We previously reported that the PRMT1v2 isoform, which is generated through inclusion of alternative exon 2, is overexpressed in breast cancer cells and promotes their invasiveness. However, the precise mechanism by which expression of this isoform is controlled and how it is dysregulated in breast cancer remains unknown. Using a custom RNA interference-based screen, we identified several RNA binding proteins (RBP) which, when knocked down, altered the relative abundance of the alternatively spliced PRMT1v2 isoform...
July 18, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28733224/celf1-preferentially-binds-to-exon-intron-boundary-and-regulates-alternative-splicing-in-hela-cells
#4
Heng Xia, Dong Chen, Qijia Wu, Gang Wu, Yanhong Zhou, Yi Zhang, Libin Zhang
The current RIP-seq approach has been developed for the identification of genome-wide interaction between RNA binding protein (RBP) and the bound RNA transcripts, but still rarely for identifying its binding sites. In this study, we performed RIP-seq experiments in HeLa cells using a monoclonal antibody against CELF1. Mapping of the RIP-seq reads showed a biased distribution at the 3'UTR and intronic regions. A total of 15,285 and 1384 CELF1-specific sense and antisense peaks were identified using the ABLIRC software tool...
July 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28730979/adsorption-of-staphylococcus-viruses-s13-and-s24-1-on-staphylococcus-aureus-strains-with-different-glycosidic-linkage-patterns-of-wall-teichoic-acids
#5
Jumpei Uchiyama, Maya Taniguchi, Kenji Kurokawa, Iyo Takemura-Uchiyama, Takako Ujihara, Hidekatsu Shimakura, Yoshihiko Sakaguchi, Hironobu Murakami, Masahiro Sakaguchi, Shigenobu Matsuzaki
The group of phages belonging to the family Podoviridae, genus P68virus, including Staphylococcus viruses S13' and S24-1, are important because of their benefits in phage therapy against Staphylococcus aureus infections. The O-glycosidic linkage patterns of wall teichoic acids (WTAs) in S. aureus cell walls seem to be important for adsorption of this phage group. In this study, the adsorption of Staphylococcus viruses S13' and S24-1 to S. aureus was examined using strains with modified WTA glycosidic linkage patterns...
July 21, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28718405/rna-binding-proteins-in-cancer-old-players-and-new-actors
#6
REVIEW
Bruno Pereira, Marc Billaud, Raquel Almeida
RNA-binding proteins (RBPs) are key players in post-transcriptional events. The combination of versatility of their RNA-binding domains with structural flexibility enables RBPs to control the metabolism of a large array of transcripts. Perturbations in RBP-RNA networks activity have been causally associated with cancer development, but the rational framework describing these contributions remains fragmented. We review here the evidence that RBPs modulate multiple cancer traits, emphasize their functional diversity, and assess future trends in the study of RBPs in cancer...
July 2017: Trends in Cancer
https://www.readbyqxmd.com/read/28716948/cooperative-repression-of-igf2r-translation-by-mir-195-and-rna-binding-protein-cugbp1
#7
Yuan Zhang, Yun Zhang, Lan Xiao, Ting-Xi Yu, Jun-Zhe Li, Jaladanki N Rao, Douglas J Turner, Myriam Gorospe, Jian-Ying Wang
Insulin-like growth factor type-2 (IGF2) receptor (IGF2R) recognizes mannose 6-phosphate-containing molecules and IGF2 and plays an important role in many pathophysiological processes including gut mucosal adaptation. However, the mechanisms that control cellular IGF2R abundance are poorly known. MicroRNAs (miRNAs) and RNA-binding proteins (RBP) critically regulate gene expression programs in mammalian cells by modulating the stability and translation of target mRNAs. Here we report that miRNA-195 (miR-195) and the RBP CUG-binding protein 1 (CUGBP1) jointly regulate IGF2R expression at the posttranscriptional level in intestinal epithelial cells...
July 17, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28710009/mrna-interactome-capture-in-mammalian-cells
#8
Nicolai Kastelic, Markus Landthaler
Throughout their entire life cycle, mRNAs are associated with RNA-binding proteins (RBPs), forming ribonucleoprotein (RNP) complexes with highly dynamic compositions. Their interplay is one key to control gene regulatory mechanisms from mRNA synthesis to decay. To assay the global scope of RNA-protein interactions, we and others have published a method combining crosslinking with highly stringent oligo(dT) affinity purification to enrich proteins associated with polyadenylated RNA (poly(A)+ RNA). Identification of the poly(A)+ RNA-bound proteome (also: mRNA interactome capture) has by now been applied to a diversity of cell lines and model organisms, uncovering comprehensive repertoires of RBPs and hundreds of novel RBP candidates...
July 11, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28702278/prima-a-gene-centered-rna-to-protein-method-for-mapping-rna-protein-interactions
#9
Alex M Tamburino, Ebru Kaymak, Shaleen Shrestha, Amy D Holdorf, Sean P Ryder, Albertha J M Walhout
Interactions between RNA binding proteins (RBPs) and mRNAs are critical to post-transcriptional gene regulation. Eukaryotic genomes encode thousands of mRNAs and hundreds of RBPs. However, in contrast to interactions between transcription factors (TFs) and DNA, the interactome between RBPs and RNA has been explored for only a small number of proteins and RNAs. This is largely because the focus has been on using 'protein-centered' (RBP-to-RNA) interaction mapping methods that identify the RNAs with which an individual RBP interacts...
2017: Translation
https://www.readbyqxmd.com/read/28701396/effects-of-the-nedd8-activating-enzyme-inhibitor-mln4924-on-lytic-reactivation-of-kaposi-s-sarcoma-associated-herpesvirus
#10
Pey-Jium Chang, Lee-Wen Chen, Li-Yu Chen, Chien-Hui Hung, Ying-Ju Shih, Shie-Shan Wang
The switch of Kaposi's sarcoma-associated herpesvirus (KSHV) from latency to lytic replication is a key event for viral dissemination and pathogenesis. MLN4924, a novel neddylation inhibitor, reportedly causes the onset of KSHV reactivation but impairs later phases of viral lytic program in infected cells. Thus far, the molecular mechanism involved in the modulation of KSHV lytic cycle by MLN4924 is not yet fully understood. Here, we confirmed that treatment of different KSHV-infected primary effusion lymphoma (PEL) cell lines with MLN4924 substantially induces viral lytic protein expression...
July 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28701145/an-in-vitro-technique-to-identify-the-rna-binding-site-sequences-for-rna-binding-proteins
#11
SunKyung Choi, Chungoo Park, Kyoon Eon Kim, Kee K Kim
RNA-protein interactions play a major role in gene regulation. Although many techniques to analyze RNA-protein interactions have been developed, noteworthy challenges such as determining the RNA sequences that bind RNA-binding proteins (RBPs) remain unsolved. Here, we describe a novel technique using a 4-thio-uridine-incorporated RNA pool to identify the RBP-binding consensus sequences for RBPs produced by in vitro transcription and translation. To confirm the fidelity of this approach, we determined the consensus RBP-binding sequence for RBFOX2, UGC(A/U)(A/U)NU, which is very similar to the known RBFOX2-binding sequence, UGCAUG...
July 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28699847/a-systematic-review-and-combined-analysis-of-therapeutic-drug-monitoring-studies-for-long-acting-risperidone
#12
Georgios Schoretsanitis, Edoardo Spina, Christoph Hiemke, Jose de Leon
This systematic review of therapeutic drug monitoring (TDM) identifies three long-acting injectable (LAI) risperidone formulations. Areas covered: Limited data is available on two formulations (RBP-7000 and in Situ Microparticle), but 20 TDM articles on the microsphere formulation were found. Risperidone TDM includes the serum concentrations of risperidone and its active metabolite, 9-hydroxyrisperidone, used for calculating: 1) the risperidone/9-hydroxyrisperidone (R/9-OH-R) ratio (a measure of CYP2D6; values >1 are indicative of a CYP2D6 poor metabolizer) and 2) the total risperidone concentration-to-dose (C/D) ratio (a measure of risperidone clearance with a normal value around 7 in oral risperidone)...
July 12, 2017: Expert Review of Clinical Pharmacology
https://www.readbyqxmd.com/read/28697339/oncogenic-activation-of-the-rna-binding-protein-nelfe-and-myc-signaling-in-hepatocellular-carcinoma
#13
Hien Dang, Atsushi Takai, Marshonna Forgues, Yotsowat Pomyen, Haiwei Mou, Wen Xue, Debashish Ray, Kevin C H Ha, Quaid D Morris, Timothy R Hughes, Xin Wei Wang
Global transcriptomic imbalance is a ubiquitous feature associated with cancer, including hepatocellular carcinoma (HCC). Analyses of 1,225 clinical HCC samples revealed that a large numbers of RNA binding proteins (RBPs) are dysregulated and that RBP dysregulation is associated with poor prognosis. We further identified that oncogenic activation of a top candidate RBP, negative elongation factor E (NELFE), via somatic copy-number alterations enhanced MYC signaling and promoted HCC progression. Interestingly, NELFE induces a unique tumor transcriptome by selectively regulating MYC-associated genes...
July 10, 2017: Cancer Cell
https://www.readbyqxmd.com/read/28696227/translational-regulation-of-viral-secretory-proteins-by-the-5-coding-regions-and-a-viral-rna-binding-protein
#14
Johan Nordholm, Jeanne Petitou, Henrik Östbye, Diogo V da Silva, Dan Dou, Hao Wang, Robert Daniels
A primary function of 5' regions in many secretory protein mRNAs is to encode an endoplasmic reticulum (ER) targeting sequence. In this study, we show how the regions coding for the ER-targeting sequences of the influenza glycoproteins NA and HA also function as translational regulatory elements that are controlled by the viral RNA-binding protein (RBP) NS1. The translational increase depends on the nucleotide composition and 5' positioning of the ER-targeting sequence coding regions and is facilitated by the RNA-binding domain of NS1, which can associate with ER membranes...
July 10, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28695513/methodology-to-identify-poly-adp-ribose-polymerase-1-parp1-mrna-targets-by-par-clip
#15
Manana Mekishvili, Elena Matveeva, Yvonne Fondufe-Mittendorf
There is a long list of important RNA-binding proteins (RBP) involved in different steps of gene expression through posttranscriptional modifications: pre-mRNA splicing, mRNA stabilization, polyadenylation, mRNA export from nucleus to the cytoplasm, and translation. The critical role of RNA-protein interaction necessitates a continuous identification of proteins involved in this process. Here we describe the identification of Poly-ADP-Ribose Polymerase 1 (PARP1) as an RNA binding protein involved in RNA splicing...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28695512/approaches-for-investigating-translational-regulation-controlled-by-parp1-biotin-based-uv-cross-linking-and-luciferase-reporter-assay
#16
Yingbiao Ji
The RNA-binding proteins (RBPs) play a pivotal role in controlling gene expression through posttranscriptional processes. As the trans-acting factors, RBPs interact with the cis-regulatory elements located within mRNAs to regulate mRNA translational efficiency. Adding a new-layer regulation, recent studies suggest that poly(ADP-ribosyl)ation of the RNA-binding proteins often inhibit the RNA-binding ability of RBPs, thus regulating RBP-dependent mRNA metabolism including translational control. Here, we describe a biotin-based UV cross-linking method to determine if excessive accumulation of pADPr in the cell disrupts the interaction between RBPs and their target mRNAs...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28675861/synthesis-of-biochar-from-residues-after-biogas-production-with-respect-to-cadmium-and-nickel-removal-from-wastewater
#17
Aleksandra Bogusz, Katarzyna Nowak, Magdalena Stefaniuk, Ryszard Dobrowolski, Patryk Oleszczuk
The objective of the study was to investigate the ability of biochars prepared under different temperatures (400 °C and 600 °C) from the residue of biogas production (RBP) for the adsorption of cadmium (Cd(II)) and nickel (Ni(II)) ions from aqueous solution. Furthermore, the RBP biochars adsorption capacity was compared with adsorption capacity of biochar produced from wheat straw at 600 °C (BCS600). The kinetics of the adsorption, the sorption isotherms, the influence of solution pH and the interfering ions (chlorides and nitrates) were investigated...
July 1, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28673966/mediator-1-contributes-to-enamel-mineralization-as-a-coactivator-for-notch1-signaling-and-stimulates-transcription-of-the-alkaline-phosphatase-gene
#18
Keigo Yoshizaki, Lizhi Hu, Thai Nguyen, Kiyoshi Sakai, Masaki Ishikawa, Ichiro Takahashi, Satoshi Fukumoto, Pamela Den Besten, Daniel D Bikle, Yuko Oda, Yoshihiko Yamada
Tooth enamel is mineralized through the differentiation of multiple dental epithelia including ameloblasts and the stratum intermedium (SI), and this differentiation is controlled by several signaling pathways. Previously, we demonstrated that the transcriptional coactivator Mediator 1 (Med1) plays a critical role in enamel formation. For instance, conditional ablation of Med1 in dental epithelia causes functional changes in incisor-specific dental epithelial stem cells, resulting in mineralization defects in the adult incisors...
July 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28658347/circulating-omentin-1-might-be-associated-with-metabolic-health-status-in-different-phenotypes-of-body-size
#19
Shahab Alizadeh, Khadijeh Mirzaei, Chonur Mohammadi, Seyed Ali Keshavarz, Zhila Maghbooli
Objective: Adipokines are mediators of body composition and are involved in obesity complications. This study aimed to assess the association of circulating omentin-1, vaspin, and RBP-4 with body composition indices and metabolic health status (MHS) in different phenotypes of body size. Subjects and methods: A total of 350 subjects were included in the current cross-sectional study. Body composition was measured using a body composition analyzer, and serum concentrations of omentin-1, vaspin, and RBP-4 were assessed by ELISA kits...
June 26, 2017: Archives of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28651078/leuconostoc-mesenteroides-and-leuconostoc-pseudomesenteroides-bacteriophages-genomics-and-cross-species-host-ranges
#20
Silvina A Pujato, Daniela M Guglielmotti, Manuel Martínez-García, Andrea Quiberoni, Francisco J M Mojica
Unveiling virus-host interactions are relevant for understanding the biology and evolution of microbes globally, but in particular, it has also a paramount impact on the manufacture of fermented dairy products. In this study, we aim at characterizing phages infecting the commonly used heterofermentative Leuconostoc spp. on the basis of host range patterns and genome analysis. Host range of six Leuconostoc phages was investigated using three methods (efficiency of plaquing, spot and turbidity tests) against Ln...
June 12, 2017: International Journal of Food Microbiology
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