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https://www.readbyqxmd.com/read/28433375/mosaicism-between-trophectoderm-and-inner-cell-mass
#1
REVIEW
Antonio Capalbo, Laura Rienzi
Defining the actual incidence and prevalence of mosaicism in human blastocysts still remains a difficult task. The small amount of evidence generated by animal and human studies does not support the existence of mechanisms involved in developmental arrest, clonal depletion, or aneuploidy rescue for abnormal cells in euploid/aneuploid embryos during preimplantation development. However, studies in humans are mainly descriptive and lack functional evidence. Understanding the biological mechanisms that beset preimplantation differentiation holds the potential to reveal the role of aneuploidies and gene dosage imbalances in cell fate decision, providing important clues on the origin and evolution of embryonic mosaicism...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28433373/preimplantation-genetic-diagnosis-for-aneuploidy-testing-in-women-older-than-44-years-a-multicenter-experience
#2
Filippo Maria Ubaldi, Danilo Cimadomo, Antonio Capalbo, Alberto Vaiarelli, Laura Buffo, Elisabetta Trabucco, Susanna Ferrero, Elena Albani, Laura Rienzi, Paolo E Levi Setti
OBJECTIVE: To report laboratory and clinical outcomes in preimplantation genetic diagnosis for aneuploidies (PGD-A) cycles for women 44 to 47 years old. DESIGN: Multicenter, longitudinal, observational study. SETTING: In vitro fertilization (IVF) centers. PATIENT(S): One hundred and thirty-seven women aged 44.7 ± 0.7 years (range: 44.0-46.7) undergoing 150 PGD-A cycles during April 2013 to January 2016. INTERVENTION(S): Quantitative polymerase chain reaction-based PGD-A on trophectoderm biopsies and cryopreserved euploid single-embryo transfer (SET)...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28433368/chromosomal-mosaicism-detected-during-preimplantation-genetic-screening-results-of-a-worldwide-web-based-survey
#3
REVIEW
Ariel Weissman, Gon Shoham, Zeev Shoham, Simon Fishel, Milton Leong, Yuval Yaron
Embryonic mosaicism, the presence of more than one distinct cell line within an embryo, has recently become the focus of growing attention and controversy in the context of preimplantation genetic screening (PGS). To evaluate the extent of mosaic aneuploidy in clinical practice and to gain insight on the practices and views regarding this issue, we conducted a survey using a prospective, 20-item Web-based questionnaire with questions related to practices and views regarding mosaicism in PGS. A total of 102 in vitro fertilization (IVF) units from 32 countries that performed 108,900 IVF cycles annually responded to the survey...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28394112/effect-of-familial-mediterranean-fever-on-ivf-outcome-a-retrospective-case-series
#4
Nafiye Karakas Yilmaz, Mustafa Kara, Metin Kaba, Bugra Coskun, Selcuk Erkilinc, Salim Erkaya
Although the in vitro fertilization-intra-cytoplasmic sperm injection (IVF-ICSI) has been utilized widely, the management in patients with an autoimmune disease is still a challenge. The aim of this study was to demonstrate IVF-ICSI outcomes in infertile women with familial Mediterranean fever (FMF). Patient data were collected from the cases registered from January 2006 until January 2014. A total of 6152 assisted reproductive technology (ART) cycles were analyzed retrospectively in the Ankara Zekai Tahir Burak Women’s Health Education and Research Hospital...
June 2016: Acta Clinica Croatica
https://www.readbyqxmd.com/read/28390691/zika-virus-detected-in-amniotic-fluid-and-umbilical-cord-blood-in-an-in%C3%A2-vitro-fertilization-conceived-pregnancy-in-venezuela
#5
Isaac Benjamin, Gissel Fernández, José Valentin Figueira, Leticia Parpacén, María Teresa Urbina, Randolfo Medina
OBJECTIVE: To describe the consequences of Zika virus infection at 10 weeks of gestation in an IVF-conceived pregnancy in Venezuela. DESIGN: A case report. SETTING: Private assisted reproduction unit. PATIENT(S): A 36-year-old patient who conceived her first pregnancy through IVF and became infected with Zika virus at 10 weeks' gestation in Venezuela. INTERVENTION(S): In vitro fertilization with fresh ET...
April 6, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28331619/processing-and-selection-of-surgically-retrieved-sperm-for-icsi-a-review
#6
Greta Verheyen, Biljana Popovic-Todorovic, Herman Tournaye
Although the technique of intracytoplasmic sperm injection (ICSI) has been a revolution in the alleviation of male infertility, the use of testicular sperm for ICSI was a formerly unseen breakthrough in the treatment of the azoospermic man with primary testicular failure. At the clinical level, different procedures of testicular sperm retrieval (conventional TESE, micro-TESE, FNA/TESA, MESA, PESA) are being performed, the choice is mainly based on the cause of azoospermia (obstructive versus non-obstructive) and the surgeon's skills...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28191261/global-dna-methylation-levels-are-altered-by-modifiable-clinical-manipulations-in-assisted-reproductive-technologies
#7
Jayashri Ghosh, Christos Coutifaris, Carmen Sapienza, Monica Mainigi
BACKGROUND: We analyzed placental DNA methylation levels at repeated sequences (LINE1 elements) and all CCGG sites (the LUMA assay) to study the effect of modifiable clinical or laboratory procedures involved in in vitro fertilization. We included four potential modifiable factors: oxygen tension during embryo culture, fresh embryo transfer vs frozen embryo transfer, intracytoplasmic sperm injection (ICSI) vs conventional insemination or day 3 embryo transfer vs day 5 embryo transfer...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28182605/assisted-reproductive-technology-surveillance-united-states-2014
#8
Saswati Sunderam, Dmitry M Kissin, Sara B Crawford, Suzanne G Folger, Denise J Jamieson, Lee Warner, Wanda D Barfield
PROBLEM/CONDITION: Since the first U.S. infant conceived with assisted reproductive technology (ART) was born in 1981, both the use of ART and the number of fertility clinics providing ART services have increased steadily in the United States. ART includes fertility treatments in which eggs or embryos are handled in the laboratory (i.e., in vitro fertilization [IVF] and related procedures). Women who undergo ART procedures are more likely than women who conceive naturally to deliver multiple-birth infants...
February 10, 2017: MMWR. Surveillance Summaries: Morbidity and Mortality Weekly Report. Surveillance Summaries
https://www.readbyqxmd.com/read/28158511/adjuncts-in-the-ivf-laboratory-where-is-the-evidence-for-add-on-interventions
#9
Joyce Harper, Emily Jackson, Karen Sermon, Robert John Aitken, Stephen Harbottle, Edgar Mocanu, Thorir Hardarson, Raj Mathur, Stephane Viville, Andy Vail, Kersti Lundin
Globally, IVF patients are routinely offered and charged for a selection of adjunct treatments and tests or 'add-ons' that they are told may improve their chance of a live birth, despite there being no clinical evidence supporting the efficacy of the add-on. Any new IVF technology claiming to improve live birth rates (LBR) should, in most cases, first be tested in an appropriate animal model, then in clinical trials, to ensure safety, and finally in a randomized controlled trial (RCT) to provide high-quality evidence that the procedure is safe and effective...
March 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28064422/improved-detection-of-mineral-oil-toxicity-using-an-extended-mouse-embryo-assay
#10
Alessandra J Ainsworth, Jolene R Fredrickson, Dean E Morbeck
PURPOSE: Successful in vitro fertilization (IVF) relies on sound laboratory methods and culture conditions which depend on sensitive quality control (QC) testing. This study aimed to improve the sensitivity of mouse embryo assays (MEA) for detection of mineral oil toxicity. METHODS: Five experiments were conducted to study modifications of the standard mouse embryo assay (MEA) in order to improve sensitivity using clinical grade mineral oil with known peroxide concentrations...
March 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28031323/inter-observer-and-intra-observer-agreement-between-embryologists-during-selection-of-a-single-day-5-embryo-for-transfer-a-multicenter-study
#11
Ashleigh Storr, Christos A Venetis, Simon Cooke, Suha Kilani, William Ledger
STUDY QUESTION: What is the inter-observer and intra-observer agreement between embryologists when selecting a single Day 5 embryo for transfer? SUMMARY ANSWER: The inter-observer and intra-observer agreement between embryologists when selecting a single Day 5 embryo for transfer was generally good, although not optimal, even among experienced embryologists. WHAT IS KNOWN ALREADY: Previous research on the morphological assessment of early stage (two pronuclei to Day 3) embryos has shown varying levels of inter-observer and intra-observer agreement...
February 2017: Human Reproduction
https://www.readbyqxmd.com/read/28017976/acute-abdomen-in-the-17th-week-of-twin-pregnancy-due-to-ovarian-torsion-a-late-complication-of-ivf
#12
D Habek, R Bauman, L Rukavina Kralj, T Hafner, T Turudic, S Vujisic
Background: A 32-year-old woman with tubal factor infertility due to bilateral laparoscopic salpingectomy conceived twins with in vitro fertilization (IVF). She developed moderate ovarian hyperstimulation syndrome which was treated with anticoagulant therapy. The subsequent course of the twin pregnancy was normal until the 17th week of gestation when she presented to hospital because of a sharp pain in the right lower abdomen which ceased after admission. Case: Except for a single incident of vomiting, patient had no other subjective symptoms...
December 2016: Geburtshilfe und Frauenheilkunde
https://www.readbyqxmd.com/read/28003868/outcome-of-one-hundred-consecutive-icsi-attempts-using-patient-operated-home-sonography-for-monitoring-follicular-growth
#13
J Gerris, F Vandekerckhove, P De Sutter
OBJECTIVE: cohort study describing clinical and laboratory outcomes in ICSI patients using self-operated endovaginal tele-monitoring (SOET). SETTING: University department of reproductive medicine. PATIENTS: 78 patients undergoing 100 consecutive ICSI attempts. INTERVENTIONS: patients' recorded vaginal sonograms and sent recordings using the cloud based Sonaura device to the care provider who procured responses, avoiding hospital visits...
September 2016: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/27909571/affordable-art-in-kenya-the-only-hope-for-involuntary-childlessness
#14
S W Ndegwa
The reported overall subfertility rate in Kenia is 26.1% with 50% attributed to tubal factors and 15% due to male factors. This is probably an underestimation taking into consideration that due to the stigma and myths of the disease, many couples are seeking alternative care from religious sects, witchdoctors, herbalists. Because the costs associated with IVF in private centres are only affordable for the happy few, the only true hope for most Kenyans struggling with unintended childlessness lies in the introduction of affordable ART services...
June 27, 2016: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/27889868/biobanking-of-different-body-fluids-within-the-frame-of-ivf-a-standard-operating-procedure-to-improve-reproductive-biology-research
#15
Michael Schenk, Berthold Huppertz, Barbara Obermayer-Pietsch, Darja Kastelic, Martina Hörmann-Kröpfl, Gregor Weiss
PURPOSE: The aim of the present study was to develop a standard operating procedure (SOP) for the collection, transport, and storage of human cumulus cells, follicular fluid, blood serum, seminal plasma, embryo culture supernatant, and embryo culture supernatant control obtained within the IVF process under approved protocols and written informed consent from participating patients. The SOP was developed at the Kinderwunsch Institut Schenk, Dobl, Austria, together with Biobank Graz of the Medical University of Graz, Austria...
February 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27844488/how-do-laboratory-embryo-transfer-techniques-affect-ivf-outcomes-a-review-of-current-literature
#16
George Sigalos, Olga Triantafyllidou, Nikos Vlahos
Over the last few years, many studies have focused on embryo selection methods, whereas little attention has been given to the standardization of the procedure of embryo transfer. In this review, several parameters of the embryo transfer procedure are examined, such as the: (i) culture medium volume and loading technique; (ii) syringe and catheters used for embryo transfer; (iii) viscosity and composition of the embryo transfer medium; (iv) environment of embryo culture; (v) timing of embryo transfer; (vi) and standardization of the embryo transfer techniques...
April 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/27827818/oocyte-embryo-and-blastocyst-cryopreservation-in-art-systematic-review-and-meta-analysis-comparing-slow-freezing-versus-vitrification-to-produce-evidence-for-the-development-of-global-guidance
#17
Laura Rienzi, Clarisa Gracia, Roberta Maggiulli, Andrew R LaBarbera, Daniel J Kaser, Filippo M Ubaldi, Sheryl Vanderpoel, Catherine Racowsky
BACKGROUND: Successful cryopreservation of oocytes and embryos is essential not only to maximize the safety and efficacy of ovarian stimulation cycles in an IVF treatment, but also to enable fertility preservation. Two cryopreservation methods are routinely used: slow-freezing or vitrification. Slow-freezing allows for freezing to occur at a sufficiently slow rate to permit adequate cellular dehydration while minimizing intracellular ice formation. Vitrification allows the solidification of the cell(s) and of the extracellular milieu into a glass-like state without the formation of ice...
March 1, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/27821560/in-vitro-fertilization-research-is-translational-research
#18
Alan H DeCherney, Rebecca L Barnett
In vitro fertilization (IVF) is the perfect example of translational research. Changes in IVF and the IVF laboratory have been transmitted to clinical care, showing dramatic improvements in health outcomes, including notable increases in the cumulative pregnancy rate. Current research in the laboratory focusing on culture media, embryo selection criteria, and implementation of genetic testing and manipulation promises to translate to further improvements in our ability to assist human reproduction. The field of IVF and ART remains a large source for clinical and scientific discovery and development, and will require the proper interested and invested personnel, occupational structuring, and funding for continued success...
December 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27816236/elevated-circulating-micro-ribonucleic-acid-mirna-200b-and-mirna-429-levels%C3%A2-in-anovulatory-women
#19
Iris Eisenberg, Neta Nahmias, Michal Novoselsky Persky, Caryn Greenfield, Debra Goldman-Wohl, Arye Hurwitz, Ronit Haimov-Kochman, Simcha Yagel, Tal Imbar
OBJECTIVE: To study the role of micro-RNA (miRNA)-200b and miRNA-429 in human ovulation and to measure their expression levels in ovulatory and anovulatory patients. DESIGN: Micro-RNA-200b and miRNA-429 expression analysis in human serum and granulosa cells at different phases of the ovulation cycle in normal cycling women and women undergoing assisted reproductive technology cycles. SETTING: University-affiliated hospital and academic research laboratory...
January 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/27793366/accurate-quantitation-of-mitochondrial-dna-reveals-uniform-levels-in-human-blastocysts-irrespective-of-ploidy-age-or-implantation-potential
#20
Andrea R Victor, Alan J Brake, Jack C Tyndall, Darren K Griffin, Christo G Zouves, Frank L Barnes, Manuel Viotti
OBJECTIVE: To accurately determine mitochondrial DNA (mtDNA) levels in human blastocysts. DESIGN: Retrospective analysis. SETTING: IVF clinic. PATIENT(S): A total of 1,396 embryos derived from 259 patients. INTERVENTION(S): Blastocyst-derived trophectoderm biopsies were tested by next-generation sequencing (NGS) and quantitative real-time polymerase chain reaction (qPCR). MAIN OUTCOME MEASURE(S): For each sample the mtDNA value was divided by the nuclear DNA value, and the result was further subjected to mathematical analysis tailored to the genetic makeup of the source embryo...
January 2017: Fertility and Sterility
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