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IVF Laboratory

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https://www.readbyqxmd.com/read/28612309/pre-implantation-genetic-diagnosis-should-we-use-icsi-for-all
#1
Baruch Feldman, Adva Aizer, Masha Brengauz, Keren Dotan, Jacob Levron, Eyal Schiff, Raoul Orvieto
OBJECTIVE: Intracytoplasmic sperm injection (ICSI) is commonly used during pre-implantation genetic diagnosis (PGD) in vitro fertilization (IVF), aiming to eliminate the risk of contamination from extraneous sperm DNA. Recently, ICSI "overuse" in non-male infertility has been doubted, since it does not offer an advantage over IVF. Prompted by the aforementioned observations, we sought to assess the accuracy of IVF vs ICSI in PGD cases, as might be reflected by a difference in the prevalence of discarded embryos as a consequent of parental contamination...
June 13, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28610639/impact-of-the-ivf-laboratory-environment-on-human-preimplantation-embryo-phenotype
#2
D K Gardner, R L Kelley
The phenotype of the human embryo conceived through in vitro fertilization (IVF), that is its morphology, developmental kinetics, physiology and metabolism, can be affected by numerous components of the laboratory and embryo culture system (which comprise the laboratory environment). The culture media formulation is important in determining embryo phenotype, but this exists within a culture system that includes oxygen, temperature, pH and whether an embryo is cultured individually or in a group, all of which can influence embryo development...
June 14, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28608497/the-effect-of-hpv-infection-on-ivf-outcomes-a-systematic-review-and-meta-analysis
#3
REVIEW
Charalampos Siristatidis, Dennis Vaidakis, Eleni Sertedaki, Wellington Martins
OBJECTIVES: Tο identify, appraise and summarize the available data, concerning the impact of HPV infection on the reproductive outcomes following in vitro fertilization (IVF). METHODS: We searched for studies on PubMed, Embase, Scopus, Lilacs and the Cochrane Central Register of Controlled Trials from inception to Mar-2017. Any type of HPV infection in the woman assessed through PCR, subfertility factors and IVF indications and protocols were considered. Our primary outcomes were live birth/ongoing pregnancy and miscarriage, while secondary included clinical and laboratory parameters...
June 13, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28600620/national-survey-on-use-of-time-lapse-imaging-systems-in-ivf-laboratories
#4
Andrey V Dolinko, L V Farland, D J Kaser, S A Missmer, C Racowsky
PURPOSE: Several time-lapse imaging (TLI) systems for non-invasive continuous monitoring of developing embryos are currently available. The present study explored the prevalence, means of acquisition, and clinical application of TLI systems in USA in vitro fertilization (IVF) laboratories. METHODS: An online cross-sectional survey of 294 USA IVF laboratory directors was conducted in February and March 2016. Those directing more than one laboratory were asked to complete the survey for their home program and for their smallest laboratory by number of IVF/intracytoplasmic sperm injection (ICSI) cycle starts...
June 10, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28583200/high%C3%A2-quality-clinical%C3%A2-grade-human-embryonic-stem-cell-lines-derived-from-fresh-discarded-embryos
#5
Jinpei Ye, Nicola Bates, Despina Soteriou, Lisa Grady, Clare Edmond, Alex Ross, Alan Kerby, Philip A Lewis, Tope Adeniyi, Ronnie Wright, Kay V Poulton, Marcus Lowe, Susan J Kimber, Daniel R Brison
BACKGROUND: Human embryonic stem cells (hESCs) hold tremendous promise for cell replacement therapies for a range of degenerative diseases. In order to provide cost-effective treatments affordable by public health systems, HLA-matched allogeneic tissue banks of the highest quality clinical-grade hESCs will be required. However only a small number of existing hESC lines are suitable for clinical use; they are limited by moral and ethical concerns and none of them apply Good Manufacturing Practice (GMP) standards to the earliest and critical stages of gamete and embryo procurement...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28575413/comprehensive-protocol-of-traceability-during-ivf-the-result-of-a-multicentre-failure-mode-and-effect-analysis
#6
L Rienzi, F Bariani, M Dalla Zorza, E Albani, F Benini, S Chamayou, M G Minasi, L Parmegiani, L Restelli, G Vizziello, A Nanni Costa
STUDY QUESTION: Can traceability of gametes and embryos be ensured during IVF? SUMMARY ANSWER: The use of a simple and comprehensive traceability system that includes the most susceptible phases during the IVF process minimizes the risk of mismatches. WHAT IS KNOWN ALREADY: Mismatches in IVF are very rare but unfortunately possible with dramatic consequences for both patients and health care professionals. Traceability is thus a fundamental aspect of the treatment...
May 31, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28553759/access-to-fertility-services-in-canada-for-hiv-positive-individuals-and-couples-a-comparison-between-2007-and-2014
#7
Carson K Lo, V Logan Kennedy, Mark H Yudin, Heather M Shapiro, Mona Loutfy
In the modern era of HIV care, a multitude of clinical needs have emerged; one such need is the growing sub-specialty of HIV and reproductive health. In 2007, a study surveying Canadian fertility clinics found limited access to fertility services for HIV-positive patients. Given the extensive efforts made to address this lack of services, a follow-up assessment was warranted. This study aimed to compare the access to Canadian fertility clinics and services for HIV-positive individuals and couples in 2014 and 2007...
May 29, 2017: AIDS Care
https://www.readbyqxmd.com/read/28540437/volatile-organic-compounds-and-good-laboratory-practices-in-the-in-vitro-fertilization-laboratory-the-important-parameters-for-successful-outcome-in-extended-culture
#8
Nupur Agarwal, Ratna Chattopadhyay, Sanghamitra Ghosh, Arpita Bhoumik, S K Goswami, Baidyanath Chakravarty
PURPOSE: This study aims to describe the role of implementing good laboratory practices to improve in vitro fertilization (IVF) outcomes which are of great interest for practitioners dealing with infertility. METHODS: Certain modifications were introduced in May 2015 in our IVF laboratory like high-efficiency particulate air CODA system, steel furniture instead of wooden, use of new disinfectants like oosafe, and restriction of personnel entry along with avoidance of cosmetics like perfume to improve pregnancy rates...
May 24, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28531319/improved-sperm-kinematics-in-semen-samples-collected-after-2-h-versus-4-7-days-of-ejaculation-abstinence
#9
H Alipour, G Van Der Horst, O B Christiansen, F Dardmeh, N Jørgensen, H I Nielsen, C Hnida
STUDY QUESTION: Does a short abstinence period of only 2 h yield spermatozoa with better motility characteristics than samples collected after 4-7 days? SUMMARY ANSWER: Despite lower semen volume, sperm concentration, total sperm counts and total motile counts, higher percentages of motile spermatozoa with higher velocity and progressiveness were detected in samples obtained after 2 h. WHAT IS KNOWN ALREADY: Most studies that have assessed the effect of abstinence periods on sperm motility parameters in men with a sperm concentration below 15 million/ml have detected a higher percentage of motile spermatozoa in samples obtained after short abstinence periods...
May 22, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28524047/oestrogen-and-progesterone-receptor-in-melanoma-and-nevi-an-immunohistochemical-study
#10
Emi Dika, Pier Alessandro Fanti, Sabina Vaccari, Elisa Capizzi, Alessio Degiovanni, Agnese Gobbi, Bianca Maria Piraccini, Simone Ribero, Carlotta Baraldi, Giulia Maria Ravaioli, Michelangelo Fiorentino, Annalisa Patrizi
The effect of hormonal stimulation and fertility treatments, as well as their role in the development of malignant melanoma (MM), is still to be determined. The aim of this study was to investigate the presence of oestrogen receptor alpha (ERα) and progesterone receptor (PR) in MM and nevi after hormonal stimulation. Immunohistochemical analyses were performed utilizing antibodies specifically directed against ERα and PR in MM and atypical nevi specimens from patients: (1) diagnosed during pregnancy, (2) diagnosed in the six months following delivery, or (3) who had undergone repetitive cycles of hormonal stimulation for in vitro fertilization in the year that preceded MM diagnosis...
May 19, 2017: European Journal of Dermatology: EJD
https://www.readbyqxmd.com/read/28485664/controlled-ovarian-stimulation-and-ivf-pregnancy-in-a-trisomy-x-carrier-with-associated-hypogonadotropic-hypogonadism
#11
Claudia Massarotti, Patrizia Fiorio, Roberto Gastaldi, Lucia Rosaia De Santis, Daniela Pastorino, Valentino Remorgida, Paola Anserini
We describe successful controlled ovarian stimulation (COS) and the first known IVF pregnancy in a trisomy X carrier with associated hypogonadotropic hypogonadism (HH) linked to a chromosome 4 double mutation in the allele of the Gonadotropins Releasing Hormone receptor (GnRHr) gene. Previous administration of low dose of gonadotropins, as recommended in patients with HH, led to poor follicular recruitment. Since trisomy X is a risk factor for diminished ovarian reserve (DOR) and premature ovarian insufficiency (POI), higher doses of gonadotropins led to better ovarian response...
May 9, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28482910/pregnancy-rates-of-day-4-and-day-5-embryos-after-culture-in-an-integrated-time-lapse-incubator
#12
Verena Holschbach, Julia Weigert, Jens Erik Dietrich, Sabine Roesner, Markus Montag, Thomas Strowitzki, Bettina Toth
BACKGROUND: The aim of this study was to compare pregnancy rates in patients undergoing IVF/ICSI with embryo transfer after 4 and 5 days of culture in a closed incubation system with integrated time-lapse imaging. METHODS: Out of n = 2207 in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI) cycles performed between January 2011 and April 2016 at a tertiary referral university hospital, a total of n = 599 IVF/ICSI cycles with prolonged embryo culture in an integrated time-lapse system (EmbryoScope(©) (Vitrolife)) until day 4 or 5 were retrospectively analyzed with regard to embryo morphology and pregnancy rates...
May 8, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28433375/mosaicism-between-trophectoderm-and-inner-cell-mass
#13
REVIEW
Antonio Capalbo, Laura Rienzi
Defining the actual incidence and prevalence of mosaicism in human blastocysts still remains a difficult task. The small amount of evidence generated by animal and human studies does not support the existence of mechanisms involved in developmental arrest, clonal depletion, or aneuploidy rescue for abnormal cells in euploid/aneuploid embryos during preimplantation development. However, studies in humans are mainly descriptive and lack functional evidence. Understanding the biological mechanisms that beset preimplantation differentiation holds the potential to reveal the role of aneuploidies and gene dosage imbalances in cell fate decision, providing important clues on the origin and evolution of embryonic mosaicism...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28433373/preimplantation-genetic-diagnosis-for-aneuploidy-testing-in-women-older-than-44-years-a-multicenter-experience
#14
Filippo Maria Ubaldi, Danilo Cimadomo, Antonio Capalbo, Alberto Vaiarelli, Laura Buffo, Elisabetta Trabucco, Susanna Ferrero, Elena Albani, Laura Rienzi, Paolo E Levi Setti
OBJECTIVE: To report laboratory and clinical outcomes in preimplantation genetic diagnosis for aneuploidies (PGD-A) cycles for women 44 to 47 years old. DESIGN: Multicenter, longitudinal, observational study. SETTING: In vitro fertilization (IVF) centers. PATIENT(S): One hundred and thirty-seven women aged 44.7 ± 0.7 years (range: 44.0-46.7) undergoing 150 PGD-A cycles during April 2013 to January 2016. INTERVENTION(S): Quantitative polymerase chain reaction-based PGD-A on trophectoderm biopsies and cryopreserved euploid single-embryo transfer (SET)...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28433368/chromosomal-mosaicism-detected-during-preimplantation-genetic-screening-results-of-a-worldwide-web-based-survey
#15
REVIEW
Ariel Weissman, Gon Shoham, Zeev Shoham, Simon Fishel, Milton Leong, Yuval Yaron
Embryonic mosaicism, the presence of more than one distinct cell line within an embryo, has recently become the focus of growing attention and controversy in the context of preimplantation genetic screening (PGS). To evaluate the extent of mosaic aneuploidy in clinical practice and to gain insight on the practices and views regarding this issue, we conducted a survey using a prospective, 20-item Web-based questionnaire with questions related to practices and views regarding mosaicism in PGS. A total of 102 in vitro fertilization (IVF) units from 32 countries that performed 108,900 IVF cycles annually responded to the survey...
May 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28394112/effect-of-familial-mediterranean-fever-on-ivf-outcome-a-retrospective-case-series
#16
Nafiye Karakas Yilmaz, Mustafa Kara, Metin Kaba, Bugra Coskun, Selcuk Erkilinc, Salim Erkaya
Although the in vitro fertilization-intra-cytoplasmic sperm injection (IVF-ICSI) has been utilized widely, the management in patients with an autoimmune disease is still a challenge. The aim of this study was to demonstrate IVF-ICSI outcomes in infertile women with familial Mediterranean fever (FMF). Patient data were collected from the cases registered from January 2006 until January 2014. A total of 6152 assisted reproductive technology (ART) cycles were analyzed retrospectively in the Ankara Zekai Tahir Burak Women’s Health Education and Research Hospital...
June 2016: Acta Clinica Croatica
https://www.readbyqxmd.com/read/28390691/zika-virus-detected-in-amniotic-fluid-and-umbilical-cord-blood-in-an-in%C3%A2-vitro-fertilization-conceived-pregnancy-in-venezuela
#17
Isaac Benjamin, Gissel Fernández, José Valentin Figueira, Leticia Parpacén, María Teresa Urbina, Randolfo Medina
OBJECTIVE: To describe the consequences of Zika virus infection at 10 weeks of gestation in an IVF-conceived pregnancy in Venezuela. DESIGN: A case report. SETTING: Private assisted reproduction unit. PATIENT(S): A 36-year-old patient who conceived her first pregnancy through IVF and became infected with Zika virus at 10 weeks' gestation in Venezuela. INTERVENTION(S): In vitro fertilization with fresh ET...
June 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28331619/processing-and-selection-of-surgically-retrieved-sperm-for-icsi-a-review
#18
Greta Verheyen, Biljana Popovic-Todorovic, Herman Tournaye
Although the technique of intracytoplasmic sperm injection (ICSI) has been a revolution in the alleviation of male infertility, the use of testicular sperm for ICSI was a formerly unseen breakthrough in the treatment of the azoospermic man with primary testicular failure. At the clinical level, different procedures of testicular sperm retrieval (conventional TESE, micro-TESE, FNA/TESA, MESA, PESA) are being performed, the choice is mainly based on the cause of azoospermia (obstructive versus non-obstructive) and the surgeon's skills...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28191261/global-dna-methylation-levels-are-altered-by-modifiable-clinical-manipulations-in-assisted-reproductive-technologies
#19
Jayashri Ghosh, Christos Coutifaris, Carmen Sapienza, Monica Mainigi
BACKGROUND: We analyzed placental DNA methylation levels at repeated sequences (LINE1 elements) and all CCGG sites (the LUMA assay) to study the effect of modifiable clinical or laboratory procedures involved in in vitro fertilization. We included four potential modifiable factors: oxygen tension during embryo culture, fresh embryo transfer vs frozen embryo transfer, intracytoplasmic sperm injection (ICSI) vs conventional insemination or day 3 embryo transfer vs day 5 embryo transfer...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28182605/assisted-reproductive-technology-surveillance-united-states-2014
#20
Saswati Sunderam, Dmitry M Kissin, Sara B Crawford, Suzanne G Folger, Denise J Jamieson, Lee Warner, Wanda D Barfield
PROBLEM/CONDITION: Since the first U.S. infant conceived with assisted reproductive technology (ART) was born in 1981, both the use of ART and the number of fertility clinics providing ART services have increased steadily in the United States. ART includes fertility treatments in which eggs or embryos are handled in the laboratory (i.e., in vitro fertilization [IVF] and related procedures). Women who undergo ART procedures are more likely than women who conceive naturally to deliver multiple-birth infants...
February 10, 2017: MMWR. Surveillance Summaries: Morbidity and Mortality Weekly Report. Surveillance Summaries
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