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IVF Laboratory

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https://www.readbyqxmd.com/read/29778554/cumulative-live-birth-rates-after-ivf-in-patients-with-polycystic-ovaries-phenotype-matters
#1
Michel De Vos, Stéphanie Pareyn, Panagiotis Drakopoulos, José M Raimundo, Ellen Anckaert, Samuel Santos-Ribeiro, Nikolaos P Polyzos, Herman Tournaye, Christophe Blockeel
RESEARCH QUESTION: Do cumulative live birth rates (CLBR) vary among women with different polycystic ovary syndrome (PCOS) phenotypes who undergo IVF/intracytoplasmic sperm injection (ICSI) treatment? DESIGN: In this retrospective cohort study, data from 567 patients undergoing an assisted reproductive technology (ART) cycle between January 2010 and December 2015 were collected. Demographical traits, cycle characteristics and clinical and laboratory data were analysed...
May 7, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29757594/transport-ivf-icsi-results-of-a-25-year-experience
#2
Fernanda G E Raffo, Jorge Blaquier
OBJECTIVE: This report presents a summary of the outcomes achieved at ART center FERTILAB in Buenos Aires, Argentina, with transport IVF-ICSI for 25 years (1990-2014). METHODS: This report included all patients submitted to oocyte retrieval for IVF-ICSI whose oocytes were transported from sites of aspiration located 0.5-58 kilometers away from the central laboratory. The numeric data herein reported were taken from annual reports submitted by our institution (Fertilab) and, for purposes of comparison, by all Argentinian centers to the Latin American Registry of Assisted Reproduction (RLA) within the same time period...
May 14, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29738181/-repeated-superovulation-induction-after-failure-in-previous-ivf-et-cycles-with-the-ultra-long-protocol-analysis-of-outcomes-of-clinical-pregnancy
#3
Bin Wang, Na Kong, Jing-Yu Liu, Ning-Yuan Zhang, Ying-Chun Zhu, Jian-Jun Zhou
Objective: To analyze the clinical outcomes of repeated superovulation induction in patients with adenomyosis or moderate to severe pelvic endometriosis after failure in previous IVF-ET cycles with the ultra-long protocol. METHODS: We retrospectively analyzed the clinical data about 37 patients with adenomyosis or moderate to severe pelvic endometriosis in our center from 2009 to 2013, who underwent repeated IVF-ET after failure in the previous cycles with the ultra-long protocol, namely by injection of 2-6 ampoules of 3...
December 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29738164/-seminal-plasma-anti-m%C3%A3-llerian-hormone-and-inhibin-b-and-serum-inhibin-b-in-predicting-the-outcome-of-routine-ivf-fertilization
#4
Yan-Fei Wang, Xin Wu, Rong Hu, Fei-Miao Wang, Shao-Tong Jia, Li-Guo Pei, Hui Wang, Xiao-E Ouyang, Ting Hu, Yun-Xing Fu
Objective: To analyze the correlations of seminal plasma (sp) anti-Müllerian hormone (spAMH) and inhibin B (spINHB) and serum INHB (serINHB) with semen parameters in oligoasthenospermia patients and explore their value in predicting the outcome of routine in vitro fertilization (IVF). METHODS: We obtained the levels of spAMH, spINHB and serINHB as well as semen parameters from 88 infertile males undergoing IVF due to oligoasthenospermia or female uterine tubal factors from August 2016 to February 2017...
November 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29688494/discordance-between-serum-anti-m%C3%A3-llerian-hormone-concentrations-and-antral-follicle-counts-not-only-technical-issues
#5
M Š Alebic, N Stojanovic, D Dewailly
STUDY QUESTION: Do patient-specific features contribute to the differences between measured serum anti-Müllerian hormone (AMH) concentrations and AMH values expected from the corresponding antral follicle count (AFC)? SUMMARY ANSWER: Patient-specific features contribute to the differences between measured AMH values and AMH values expected from the corresponding AFC (AMHp), potentially through their effect on follicular AMH production. WHAT IS KNOWN ALREADY: Both patient counselling and patient management could be hampered by finding of disagreement between AFC and AMH if both are used for the prediction of ovarian response...
April 24, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29656830/cairo-consensus-on-the-ivf-laboratory-environment-and-air-quality-report-of-an-expert-meeting
#6
D Mortimer, J Cohen, S T Mortimer, M Fawzy, D H McCulloh, D E Morbeck, X Pollet-Villard, R T Mansour, D R Brison, A Doshi, J C Harper, J E Swain, A V Gilligan
This proceedings report presents the outcomes from an international Expert Meeting to establish a consensus on the recommended technical and operational requirements for air quality within modern assisted reproduction technology (ART) laboratories. Topics considered included design and construction of the facility, as well as its heating, ventilation and air conditioning system; control of particulates, micro-organisms (bacteria, fungi and viruses) and volatile organic compounds (VOCs) within critical areas; safe cleaning practices; operational practices to optimize air quality while minimizing physicochemical risks to gametes and embryos (temperature control versus air flow); and appropriate infection-control practices that minimize exposure to VOC...
March 2, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29609229/-cumulative-live-birth-rates-per-oocytes-retrieved-cycle-evaluation-of-clinical-outcomes-of-ivf-icsi
#7
C X Wu, T Zhang, L Shu, J Huang, F Y Diao, W Ding, Y Gao, W Wang, Y D Mao, Y G Cui, J Y Liu
Objective: Using of cumulative live birth rate (CLBR) per oocytes retrieved cycle, to assess the clinical outcomes of in vitro fertilization or intracytoplasmic sperm injection (IVF/ICSI) , and to explore impact factors on CLBR following utilization of all fresh and frozen embryos in one complete IVF/ICSI cycle using gonadotropin-releasing hormone (GnRH) agonist, GnRH-antagonist and clomiphene mild stimulation protocols. Methods: Of the patients who underwent IVF/ICSI from January 1st, 2014 to December 31st, 2015 in the First Affiliated Hospital, Nanjing Medical University, a total of 6 142 oocytes retrieved cycles were included...
March 25, 2018: Zhonghua Fu Chan Ke za Zhi
https://www.readbyqxmd.com/read/29572693/human-chorionic-gonadotropin-serum-levels-following-ovulation-triggering-and-ivf-cycle-outcome
#8
Yossi Mizrachi, Eran Horowitz, Jacob Farhi, David Levran, Arieh Raziel, Ariel Weissman
PURPOSE: The clinical significance of serum hCG levels after ovulation triggering was studied previously with conflicting results. Our aim was to study the correlation of hCG levels on the day after ovulation triggering using recombinant hCG (r-hCG) with treatment outcome. METHODS: A prospective observational study of all fresh IVF/ICSI cycles in a single medical center, between January 2015 and June 2016, was performed. hCG serum levels were obtained 10-12 h following ovulation triggering with 250 mcg r-hCG...
March 23, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29565958/the-morphology-and-possible-clinical-significance-of-the-intraforaminal-ligaments-in-the-entrance-zones-of-the-l1-l5-levels
#9
Enyi Zhong, Qinghao Zhao, Benchao Shi, Yushan Xie, Zihai Ding, Hai Lv, Shizhen Zhong, Wenhua Huang
BACKGROUND: The extraforaminal ligaments between the L1-L5 lumbar spinal nerves and the tissues surrounding the intervertebral foramina (IVF) have been well studied. However, little research has been performed to describe the local anatomy of the entrance zones at the L1-L5 level. Detailed anatomic studies of the intraforaminal ligaments (IFLs) in the entrance zones at the L1-L5 levels have not been performed. OBJECTIVES: The objective of this study is to identify and describe the IFLs in the entrance zones of the L1-L5 IVF and to determine their possible clinical significance...
March 2018: Pain Physician
https://www.readbyqxmd.com/read/29533761/evaluation-of-sperm-motility-with-casa-mot-which-factors-may-influence-our-measurements
#10
Marc Yeste, Sergi Bonet, Joan E Rodríguez-Gil, Maria M Rivera Del Álamo
Computer-aided sperm analysis (CASA) is now routinely used in IVF clinics, animal breeding centres and research laboratories. Although CASA provides a more objective way to evaluate sperm parameters, a significant number of factors can affect these measurements. This paper classifies these factors into four categories: (1) sample and slide (e.g. preincubation time, type of specimen and type of chamber slide); (2) microscope (e.g. light source and microscope stage); (3) hardware and software, including the settings of each system; and (4) user-related factors...
March 14, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29481618/impact-of-diet-and-bariatric-surgery-on-anti-m%C3%A3-llerian-hormone-levels
#11
E Nilsson-Condori, J L Hedenbro, A Thurin-Kjellberg, A Giwercman, B Friberg
STUDY QUESTION: Do serum levels of anti-Müllerian hormone (AMH) change in women of reproductive age following dietary and surgery-induced weight loss? SUMMARY ANSWER: AMH levels increased after very low-calorie diet (VLCD) before surgery and decreased at 6 and 12 months after Roux-en-Y gastric bypass (RYGB), beyond expected normal age-related decline. WHAT IS KNOWN ALREADY: Obesity has negative effects on fertility and IVF outcomes, and possibly also on AMH levels...
April 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29452361/iui-review-and-systematic-assessment-of-the-evidence-that-supports-global-recommendations
#12
Ben Cohlen, Aartjan Bijkerk, Sheryl Van der Poel, Willem Ombelet
BACKGROUND: IUI with or without ovarian stimulation (OS) has become a first-line treatment option for many infertile couples, worldwide. The appropriate treatment modality for couples and their clinical management through IUI or IUI/OS cycles must consider maternal and perinatal outcomes, most notably the clinical complication of higher-order multiple pregnancies associated with IUI-OS. With a current global emphasis to continue to decrease maternal and perinatal mortality and morbidity, the World Health Organization (WHO) had established a multi-year project to review the evidence for the establishment of normative guidance for the implementation of IUI as a treatment to address fertility problems, and to consider its cost-effectiveness for lower resource settings...
May 1, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29447147/assisted-reproductive-technology-surveillance-united-states-2015
#13
Saswati Sunderam, Dmitry M Kissin, Sara B Crawford, Suzanne G Folger, Sheree L Boulet, Lee Warner, Wanda D Barfield
PROBLEM/CONDITION: Since the first U.S. infant conceived with assisted reproductive technology (ART) was born in 1981, both the use of ART and the number of fertility clinics providing ART services have increased steadily in the United States. ART includes fertility treatments in which eggs or embryos are handled in the laboratory (i.e., in vitro fertilization [IVF] and related procedures). Although the majority of infants conceived through ART are singletons, women who undergo ART procedures are more likely than women who conceive naturally to deliver multiple-birth infants...
February 16, 2018: MMWR. Surveillance Summaries: Morbidity and Mortality Weekly Report. Surveillance Summaries
https://www.readbyqxmd.com/read/29325603/culture-conditions-affect-ca2-release-in-artificially-activated-mouse-and-human-oocytes
#14
Yuechao Lu, Davina Bonte, Minerva Ferrer-Buitrago, Mina Popovic, Jitesh Neupane, Margot Van der Jeught, Luc Leybaert, Petra De Sutter, Björn Heindryckx
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the pattern of Ca2+ release and embryonic developmental potential. In the mouse, application of 5μM ionomycin in potassium simplex optimisation medium (KSOM) or 10µM ionomycin in Ca2+-free KSOM significantly reduced the Ca2+ flux and resulted in failure of blastocyst formation compared with 10μM ionomycin in KSOM...
January 12, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29318868/embryo-development-until-blastocyst-stage-with-and-without-renewal-of-single-medium-on-day-3
#15
Fernando Peña, Rocío Dávalos, Adolfo Rechkemmer, Alvaro Ascenzo, Mauricio Gonzales
OBJECTIVE: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage. METHODS: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3)...
March 1, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29314770/calibration-of-redox-potential-in-sperm-wash-media-and-evaluation-of-oxidation-reduction-potential-values-in-various-assisted-reproductive-technology-culture-media-using-mioxsys-system
#16
M K Panner Selvam, R Henkel, R Sharma, A Agarwal
Oxidation-reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to support the development of fertilized oocytes under in vitro conditions. The composition and conditions of these media are vital for optimal functioning of the gametes. Currently, there are no data on the status of redox potential of sperm processing and assisted reproduction media. The purpose of this study was to compare the oxidation-reduction potential values of the different media and to calibrate the oxidation-reduction potential values of the sperm wash medium using oxidative stress inducer cumene hydroperoxide and antioxidant ascorbic acid...
March 2018: Andrology
https://www.readbyqxmd.com/read/29305844/progesterone-rise-on-hcg-day-is-negatively-correlated-with-ivf-et-outcomes-in-natural-cycles
#17
Xiadi Wu, Yundong Mao, Yan Gao, Xiaoqiao Qian, Wei Wang, Wei Ding, Shi-Wen Jiang, Jiayin Liu
OBJECTIVE: To investigate the effect of progesterone rise on hCG day on the laboratory and clinical outcomes in natural cycles and to explore the possible factors related to the occurrence of progesterone elevation. PATIENTS AND STUDY METHODS: Retrospective analysis was performed in 1157 infertile women with decreased ovarian reserve. Eligible infertile women undergoing IVF in natural cycles were assigned to four groups according to serum progesterone levels on the day of hCG administration: group 1, P<2nmol/L; group 2, 2<P<2...
March 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29204869/estrogen-treatment-in-infertile-women-with-premature-ovarian-insufficiency-in-transitional-phase-a-retrospective-analysis
#18
Sara Pinelli, Paolo Giovanni Artini, Stefano Basile, Maria Elena Rosa Obino, Claudia Sergiampietri, Diana Giannarelli, Giovanna Simi, Vito Cela
PURPOSE: During the transitional phase of premature ovarian insufficiency (POI), sporadic resumption of ovulation is possible because of fluctuation of hormonal levels but the chance of spontaneous pregnancy is low, and the main perspective of childbearing in these women is egg donation or adoption. The purpose of the study was to verify whether treatment with estrogens in POI patients in transitional phase could reduce FSH levels and to evaluate if this pre-treatment could improve reproductive outcomes of in vitro fertilization (IVF)...
March 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29198847/first-delivery-in-a-leukemia-survivor-after-transplantation-of-cryopreserved-ovarian-tissue-evaluated-for-leukemia-cells-contamination
#19
Moran Shapira, Hila Raanani, Iris Barshack, Ninette Amariglio, Sanaz Derech-Haim, Meital Nagar Marciano, Eyal Schiff, Raoul Orvieto, Dror Meirow
OBJECTIVE: To describe a successful autologous ovarian tissue re-transplantation in a sterile leukemia survivor after evaluation for minimal residual disease and provide a review of the current literature. DESIGN: Presentation of a carefully designed workup taken to evaluate tissue for minimal residual disease, its limitations, and applicability to other patients. To date, there have not been any publications of auto-transplantations in leukemia survivors, owing to an estimated high risk for malignancy induction...
January 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29162640/outcomes-of-a-clinical-pathway-to-standardize-use-of-maintenance-intravenous-fluids
#20
Sahar N Rooholamini, Holly Clifton, Wren Haaland, Caitlin McGrath, Surabhi B Vora, Claudia S Crowell, Holly Romero, Jeffrey Foti
OBJECTIVES: Improper use of maintenance intravenous fluids (IVFs) may cause serious hospital-acquired harm. We created an evidence-based clinical pathway to guide providers on the indications for IVF, its preferred composition, and appropriate clinical monitoring. METHODS: Pathway implementation was supported by the creation of an electronic order set (PowerPlan) and hospital-wide education. Outcomes were measured among pathway-eligible patients for the years before (July 1, 2014-June 30, 2015) and after (July 1, 2015-June 30, 2016) implementation...
December 2017: Hospital Pediatrics
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