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IVF embryo pH

Petra L Wale, David K Gardner
BACKGROUND: Although laboratory procedures, along with culture media formulations, have improved over the past two decades, the issue remains that human IVF is performed in vitro (literally 'in glass'). METHODS: Using PubMed, electronic searches were performed using keywords from a list of chemical and physical factors with no limits placed on time. Examples of keywords include oxygen, ammonium, volatile organics, temperature, pH, oil overlays and incubation volume/embryo density...
January 2016: Human Reproduction Update
Karen C Hazzard, Dawn E Watkins-Chow, Lisa J Garrett
In vitro fertilization (IVF) is used to produce mouse embryos for a variety of reasons. We evaluated the effect of the method of euthanasia on the fertilization rate in 2 different IVF protocols. Oocytes collected from C57BL/6J female mice euthanized by CO2 inhalation or cervical dislocation were used in IVF with fresh sperm from either wild-type or genetically engineered C57BL/6J. Compared with CO2 inhalation, cervical dislocation improved the resulting rate of fertilization by 18% in an IVF method using Cook media and by 13% in an IVF method using methyl-B cyclodextrin and reduced glutathione...
November 2014: Journal of the American Association for Laboratory Animal Science: JAALAS
Ashok Agarwal, Damayanthi Durairajanayagam, Stefan S du Plessis
Assisted reproductive technology (ART) is a common treatment of choice for many couples facing infertility issues, be it due to male or female factor, or idiopathic. Employment of ART techniques, however, come with its own challenges as the in vitro environment is not nearly as ideal as the in vivo environment, where reactive oxygen species (ROS) build-up leading to oxidative stress is kept in check by the endogenous antioxidants system. While physiological amounts of ROS are necessary for normal reproductive function in vivo, in vitro manipulation of gametes and embryos exposes these cells to excessive ROS production either by endogenous or exogenous environmental factors...
2014: Reproductive Biology and Endocrinology: RB&E
Ghodrat Ebadi Manas, Shapour Hasanzadeh, Golamreza Najafi, Kazem Parivar, Parichehr Yaghmaei
BACKGROUND: Pyridaben, a pyridazinone derivative, is a new acaricide and insecticide for control of mites and some insects such as white flies, aphids and thrips. OBJECTIVE: This study was designed to elucidate how pyridaben can affect the sperms' morphological parameters, its DNA integrity, and to estimate the effect of various quantities of pyridaben on in vitro fertilization rate. MATERIALS AND METHODS: In this study, 80 adult male Balb/C strain mice were used...
August 2013: Iranian Journal of Reproductive Medicine
Margot Van der Jeught, Björn Heindryckx, Thomas O'Leary, Galbha Duggal, Sabitri Ghimire, Sylvie Lierman, Nadine Van Roy, Susana M Chuva de Sousa Lopes, Tom Deroo, Dieter Deforce, Petra De Sutter
STUDY QUESTION: Is there an effect of the TGFβ inhibitor SB431542 (SB) on the epiblast compartment of human blastocysts, and does it affect subsequent human embryonic stem cell (hESC) derivation? SUMMARY ANSWER: SB increases the mean number of NANOG-positive cells in the inner cell mass (ICM), and allows for subsequent hESC derivation. WHAT IS KNOWN ALREADY: It is known that inhibition of TGFβ by SB has a positive effect on mouse ESC self-renewal, while active TGFβ signalling is needed for self-renewal of primed ESC...
January 2014: Human Reproduction
Woo-Sung Kwon, Yoo-Jin Park, El-Sayed A Mohamed, Myung-Geol Pang
OBJECTIVE: To examine how voltage-dependent anion channels (VDACs) regulate sperm function in capacitation conditions. DESIGN: Experimental prospective study. SETTING: Academic research laboratory. ANIMAL(S): Male ICR and female B6D2F1/CrljOri mice (8-12 weeks old). INTERVENTION(S): Female mice were superovulated with 5 IU of pregnant mare serum gonadotropin given IP and 5 IU of hCG given IP 48 hours later...
February 2013: Fertility and Sterility
J E Swain
BACKGROUND: Reducing environmental stress imposed upon gametes and embryos in the IVF laboratory is crucial in optimizing culture conditions and development. One environmental parameter of particular importance is external pH (pHe) of culture media. An optimal pHe has not been identified. METHODS: Electronic searches were performed using keywords focused on pH and the embryo using PUBMED through August 2011, with no limits placed on a beginning time. Examples of keywords include CO(2), bicarbonate and hydrogen ion...
May 2012: Human Reproduction Update
Matthew A Will, Natalie A Clark, Jason E Swain
PURPOSE: Minimizing environmental stress helps maintain cellular homeostasis and is a crucial component in optimizing embryo development in vitro and resulting ART success. One stressor of particular interest is pH. Biologic buffers, such as HEPES and MOPS, are valuable tools for stabilizing pH. The objective of this manuscript is to summarize efficacy and impact of various pH buffers used during IVF lab procedures METHODS: Keyword searches were performed using Pubmed and Medline and relevant literature reviewed...
August 2011: Journal of Assisted Reproduction and Genetics
Martha Hentemann, Karim Mousavi, Kjell Bertheussen
OBJECTIVE: To determine the optimum pH in sequential media for embryo culture around the fertilization-zygote stage and cleavage stage, with use of a mouse embryo assay. DESIGN: Experimental laboratory study. SETTING: University Hospital and University Research Unit. ANIMAL(S): F1 hybrids between CD1 female and BDF male mice. INTERVENTION(S): Fertilized, one-cell mouse embryos were cultured 5 days in test media where pH was changed at defined time intervals...
March 15, 2011: Fertility and Sterility
Eric Scott Sills, Lyubov O Mykhaylyshyn, Ulyana S Dorofeyeva, David J Walsh, Umme Salma, Ahmed B Omar, Graham D Coull, Ileana A David, Kathy M Brickell, Olga M Tsar, Anthony Ph Walsh
BACKGROUND: This investigation describes features of patients undergoing in vitro fertilisation (IVF) and embryo transfer (ET) where both gametes were obtained from anonymous donors. METHODS: Gamete unsuitability or loss was confirmed in both members of seven otherwise healthy couples presenting for reproductive endocrinology consultation over a 12-month interval in Ireland. IVF was undertaken with fresh oocytes provided by anonymous donors in Ukraine; frozen sperm (anonymous donor) was obtained from a licensed tissue establishment...
2010: Reproductive Health
Jason E Swain
Supplying and maintaining appropriate culture conditions is critical to minimize stress imposed upon gametes and embryos and to optimize the in-vitro environment. One parameter that requires close scrutiny in this endeavour is pH. Though embryos have a limited ability to regulate their internal pH (pH(i)), oocytes lack robust mechanisms. Thus, careful attention to external pH (pH(e)) of culture media is imperative in IVF. Ability to withstand deviations in hydrogen ion concentration varies depending on culture conditions, as well as laboratory procedures...
July 2010: Reproductive Biomedicine Online
Eric Scott Sills, Adam C Brady, Ahmed B Omar, David J Walsh, Umme Salma, Anthony Ph Walsh
BACKGROUND: Premature ovarian failure (POF) remains a clinically challenging entity because in vitro fertilisation (IVF) with donor oocytes is currently the only treatment known to be effective. METHODS: A 33 year-old nulligravid patient with a normal karyotype was diagnosed with POF; she had a history of failed fertility treatments and had an elevated serum FSH (42 mIU/ml). Oocytes donated by her dizygotic twin sister were used for IVF. The donor had already completed a successful pregnancy herself and subsequently produced a total of 10 oocytes after a combined FSH/LH superovulation regime...
2010: Reproductive Biology and Endocrinology: RB&E
Jason E Swain, Thomas B Pool
Maintenance of stable pH is important for optimizing gamete and embryo culture. One method to stabilize pH entails using zwitterionic buffers in IVF handling media used outside the laboratory incubator. Current handling media utilize single buffers, such as MOPS or HEPES. However, the use of a single buffer limits the ability to adjust the range of buffering capacity. Furthermore, changes in temperature alter buffering of these compounds. Therefore, traditional IVF handling media utilizing a single buffer may not provide ideal pH buffering...
June 2009: Reproductive Biomedicine Online
Neoklis A Georgopoulos, George Adonakis, Vasilios Papadopoulos, George A Vagenakis, Athanasios Tsoukas, George Decavalas
We report a case of a 40-year-old primipara Caucasian woman with Turner syndrome (TS) and Hashimoto thyroiditis who had underwent a successful IVF-ET cycle with oocyte donation and single embryo transfer. All pregnancies in women with TS are considered as high risk, with cardiovascular complications being the most dangerous. Our case represents a typical case of fetal growth restriction with gradual slowing down of fetal growth after 28 weeks. At 37 + 3 gestational weeks, a healthy male newborn weighing 2240 g, with artery pH of 7...
June 2009: Gynecological Endocrinology
Zhi Qiang Fan, Yan Ping Wang, Chang Liang Yan, Lun Suo, Shi En Zhu
Cryopreservation of mouse spermatozoa has been widely used; however, fertility of frozen spermatozoa in some strains, especially when inseminating cryopreserved oocytes, is low and may be improved by assisted fertilization techniques. The present study was performed to investigate the effect of partial zona pellucida (ZP) digestion on the in vitro fertilization (IVF) capacity of frozen mouse spermatozoa. Mouse oocytes were subjected to partial ZP digestion using acidic Tyrode's solution (pH 3.1). Fertilization rates in digestion groups (30 or 45 s) were higher (P < 0...
January 2009: Laboratory Animals
Theodore L Tollner, Catherine A Vandevoort, Ashley I Yudin, Cathy A Treece, James W Overstreet, Gary N Cherr
Capacitation of macaque sperm in vitro has been achieved efficiently only with the addition of both cyclic nucleotides and methylxanthines. The use of these exogenous sperm activators clouds an understanding of the normal mechanisms underlying capacitation and may slow early embryo development following in vitro fertilization (IVF). We demonstrate that culture medium which simulates periovulatory oviductal fluid with respect to bicarbonate (HCO(3)(-)) and glucose concentration induces capacitation in a high percentage of macaque sperm as determined by the ability of sperm to undergo both the release of coating protein DEFB126 and the zona pellucida-induced acrosome reaction (AR)...
May 2009: Molecular Reproduction and Development
Manabu Ozawa, Takashi Nagai, Hiroyuki Kaneko, Junko Noguchi, Katsuhiko Ohnuma, Kazuhiro Kikuchi
We investigated the effects of HEPES in the medium (to maintain pH) and paraffin oil covering the medium (to maintain osmolality) on the developmental ability of porcine embryos produced in vitro using tightly closed glass tubes in the absence of a CO2 gas-regulated incubator. Putative porcine zygotes obtained by in vitro fertilization (IVF) of in vitro-matured (IVM) oocytes (day of IVF=Day 0) were cultured in 5% CO2 gas-equilibrated NCSU-37 media containing pyruvate and lactate during Days 0-2, and glucose during Days 2-6, in open glass tubes in a CO2 incubator or tightly closed glass tubes without a CO2 incubator at 38...
March 1, 2006: Theriogenology
Michelle Lane, David K Gardner
An inability to regulate ionic and metabolic homeostasis is related to a reduction in the developmental capacity of the embryo. The early embryo soon after fertilisation and up until compaction appears to have a reduced capacity to regulate its homeostasis. The reduced ability to regulate homeostasis, such as intracellular pH and calcium levels, by the precompaction-stage embryo appears to impact on the ability to regulate mitochondrial function and maintain adequate levels of energy production. This reduction in ATP production causes a cascade of events leading to disrupted cellular function and, perhaps ultimately, disrupted epigenetic regulation and aberrant placental and fetal development...
2005: Reproduction, Fertility, and Development
Masahiro Sato, Aki Ishikawa
To explore optimal conditions for in vitro sperm survival, we examined the effects of several media used for murine egg culture and in vitro fertilization (IVF; including M16, M2, PB1, TYH, and CZB) on motility of murine spermatozoa stored at 22 degrees C under paraffin oil. Of media tested, M2 medium, that had been adjusted to pH 7.2 by adding N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES), was found to be the best. Addition of various concentrations of HEPES to TYH did not improve sperm survival, suggesting that HEPES (and probably neutral pH) do not enhance survival of murine sperm...
May 2004: Theriogenology
B D Bavister
Research studies using animal gametes led to the first documented human IVF in 1969. A key contribution was the development of a reliable culture medium for IVF of hamster oocytes, which was then used successfully with human gametes in the laboratory. This article describes how this was accomplished. Hamster IVF was obtained using a bicarbonate-buffered culture medium based on Tyrode's solution. A dose-response experiment showed that IVF in this species was highly dependent on the pH of the culture medium. At pH 7...
2002: Reproductive Biomedicine Online
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