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Single step embryo culture

Fernando Bronet, David Agudo
PURPOSE OF REVIEW: To find the way of having more and better blastocyst is essential. How to culture embryos up to blastocyst stage remains critical. RECENT FINDINGS: Several studies show how a blastocyst score can predict the implantation potential. If that score is enough to choose the best blastocyst, as culture conditions would not be affected in these days, we would not need to check early cleavage embryos, even it could be better for the embryo development...
March 6, 2017: Current Opinion in Obstetrics & Gynecology
Dean E Morbeck, Nikola A Baumann, Devin Oglesbee
OBJECTIVE: To determine compositions of commercial single-step culture media and test with a murine model whether differences in composition are biologically relevant. DESIGN: Experimental laboratory study. SETTING: University-based laboratory. ANIMAL(S): Inbred female mice were superovulated and mated with outbred male mice. INTERVENTION(S): Amino acid, organic acid, and ions content were determined for single-step culture media: CSC, Global, G-TL, and 1-Step...
April 2017: Fertility and Sterility
Mohamed Fawzy, Mohamed Sabry, Mohamed Nour, Mohamed Y Abdelrahman, Eman Roshdy, Yasmin Magdi, Hazem Abdelghafar
OBJECTIVE: To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development. DESIGN: Comparative study. SETTING: Two private centers. PATIENT(S): The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients. INTERVENTION(S): Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium...
February 2017: Fertility and Sterility
Karen Sermon
Preimplantation genetic diagnosis (PGD) was introduced as an alternative to prenatal diagnosis: embryos cultured in vitro were analysed for a monogenic disease and only disease-free embryos were transferred to the mother, to avoid the termination of pregnancy with an affected foetus. It soon transpired that human embryos show a great deal of acquired chromosomal abnormalities, thought to explain the low success rate of IVF - hence preimplantation genetic testing for aneuploidy (PGT-A) was developed to select euploid embryos for transfer...
January 2017: Expert Review of Molecular Diagnostics
E Gómez, M Muñoz, C Simó, C Ibáñez, S Carrocera, D Martín-González, A Cifuentes
Metabolic differences between early male and female embryos can be reflected in culture medium (CM). We used a single bovine embryo culture step (24h) supporting improved birth rates under chemically defined conditions (CDC) to investigate biomarker detection of embryonic sex in contrast to classical BSA-containing medium. In vitro matured slaughterhouse oocytes were fertilized in vitro with a single bull. Embryos were initially cultured in synthetic oviduct fluid with BSA. On day-6, morulae were cultured individually in droplets with (BSA) or without protein (CDC)...
November 25, 2016: Journal of Chromatography. A
Séverine Le Gac, Verena Nordhoff
The optimization of in-vitro culture conditions and the selection of the embryo(s) with the highest developmental competence are essential components in an ART program. Culture conditions are manifold and they underlie not always evidence-based research but also trends entering the IVF laboratory. At the moment, the idea of using sequential media according to the embryo requirements has been given up in favor of the use of single step media in an uninterrupted manner due to practical issues such as time-lapse incubators...
April 1, 2017: Molecular Human Reproduction
Ioannis A Sfontouris, Wellington P Martins, Carolina O Nastri, Iara G R Viana, Paula A Navarro, Nick Raine-Fenning, Sheryl van der Poel, Laura Rienzi, Catherine Racowsky
PURPOSE: The purpose of this study was to undertake a review of the available evidence comparing the use of a single medium versus sequential media for embryo culture to the blastocyst stage in clinical IVF. METHODS: We searched the Cochrane Central, PubMed, Scopus,, Current Controlled Trials and WHO International Clinical Trials Registry Platform to identify randomized controlled trials comparing single versus sequential media for blastocyst culture and ongoing pregnancy rate...
October 2016: Journal of Assisted Reproduction and Genetics
Antonio Capalbo, Valeria Romanelli, Danilo Cimadomo, Laura Girardi, Marta Stoppa, Lisa Dovere, Domenico Dell'Edera, Filippo Maria Ubaldi, Laura Rienzi
For an IVF clinic that wishes to implement preimplantation genetic diagnosis for monogenic diseases (PGD) and for aneuploidy testing (PGD-A), a global improvement is required through all the steps of an IVF treatment and patient care. At present, CCS (Comprehensive Chromosome Screening)-based trophectoderm (TE) biopsy has been demonstrated as a safe, accurate and reproducible approach to conduct PGD-A and possibly also PGD from the same biopsy. Key challenges in PGD/PGD-A implementation cover genetic and reproductive counselling, selection of the most efficient approach for blastocyst biopsy as well as of the best performing molecular technique to conduct CCS and monogenic disease analysis...
October 2016: Journal of Assisted Reproduction and Genetics
Demián Glujovsky, Cynthia Farquhar
ET is a critical step in an assisted reproduction cycle. Over the past decade there has been an increasing trend to extending culture from cleavage-stage to blastocyst transfer. There has also been a trend to single ET and reporting the success of an assisted reproductive cycle as a cumulative live-birth rate after using both fresh and frozen embryos. There is low evidence that fresh blastocyst transfer is associated with improved live-birth rates compared with fresh cleavage-stage embryos. However, in the few studies that report cumulative pregnancy rates after fresh and frozen transfers, no significant difference was found...
August 2016: Fertility and Sterility
N Gatimel, M Z Lacroix, S Chanthavisouk, N Picard-Hagen, V Gayrard, J Parinaud, R D Léandri
STUDY QUESTION: Do the embryo culture media and plastic materials used during assisted reproductive technology (ART) laboratory procedures expose embryos to bisphenol A (BPA)? SUMMARY ANSWER: BPA was not detected in embryo culture media or protein supplements at concentrations above those encountered in normal patient serum and follicular fluids. WHAT IS KNOWN ALREADY: BPA is strongly suspected of altering the epigenome during mammalian development...
July 2016: Human Reproduction
Yohan Kim, Karla C Williams, Carson T Gavin, Emily Jardine, Ann F Chambers, Hon S Leong
Cancer cell 'invasiveness' is one of the main driving forces in cancer metastasis, and assays that quantify this key attribute of cancer cells are crucial in cancer metastasis research. The research goal of many laboratories is to elucidate the signaling pathways and effectors that are responsible for cancer cell invasion, but many of these experiments rely on in vitro methods that do not specifically simulate individual steps of the metastatic cascade. Cancer cell extravasation is arguably the most important example of invasion in the metastatic cascade, whereby a single cancer cell undergoes transendothelial migration, forming invasive processes known as invadopodia to mediate translocation of the tumor cell from the vessel lumen into tissue in vivo...
May 2016: Nature Protocols
Céline Bouillon, Roger Léandri, Laurent Desch, Alexandra Ernst, Céline Bruno, Charline Cerf, Alexandra Chiron, Céline Souchay, Antoine Burguet, Clément Jimenez, Paul Sagot, Patricia Fauque
In animal studies, extensive data revealed the influence of culture medium on embryonic development, foetal growth and the behaviour of offspring. However, this impact has never been investigated in humans. For the first time, we investigated in depth the effects of embryo culture media on health, growth and development of infants conceived by In Vitro Fertilization until the age of 5 years old. This single-centre cohort study was based on an earlier randomized study. During six months, in vitro fertilization attempts (No...
2016: PloS One
M Durand, N Sermondade, C Herbemont, J Benard, H Gronier, J Boujenah, I Cédrin-Durnerin, C Poncelet, M Grynberg, C Sifer
OBJECTIVE: The aim of this study was to compare embryo development cultured in two single-step media commercially available: Fert/Sage One Step® (Origio) and Continuous Single Culture® (CSC) (Irvine Scientific). METHODS: A prospective auto-controlled study of sibling oocytes from women undergoing conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) was performed in our center from February to June 2015. After fertilization, for every patient, half of oocytes were cultured in the single-step Fert/Sage One Step® (serie SAGE) and the other half in the single-step CSC®(serie CSC)...
March 2016: Gynécologie, Obstétrique & Fertilité
Nuno Costa-Borges, Marta Bellés, Marcos Meseguer, Daniela Galliano, Agustin Ballesteros, Gloria Calderón
OBJECTIVE: To evaluate the efficiency of using a continuous (one-step) protocol with a single medium for the culture of human embryos in a time-lapse incubator (TLI). DESIGN: Prospective cohort study on sibling donor oocytes. SETTING: University-affiliated in vitro fertilization (IVF) center. PATIENT(S): Embryos from 59 patients. INTERVENTION(S): Culture in a TLI in a single medium with or without renewal of the medium on day-3...
March 2016: Fertility and Sterility
Tai-lang Yin, Yi Zhang, Sai-jiao Li, Meng Zhao, Jin-li Ding, Wang-ming Xu, Jing Yang
Whether the type of culture media utilized in assisted reproductive technology has impacts on laboratory outcomes and birth weight of newborns in in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) was investigated. A total of 673 patients undergoing IVF/ICSI and giving birth to live singletons after fresh embryo transfer on day 3 from Jan. 1, 2010 to Dec. 31, 2012 were included. Three types of culture media were used during this period: Quinn's Advantage (QA), Single Step Medium (SSM), and Continuous Single Culture medium (CSC)...
December 2015: Journal of Huazhong University of Science and Technology. Medical Sciences
Thorir Hardarson, Mona Bungum, Joe Conaghan, Marius Meintjes, Samuel J Chantilis, Laszlo Molnar, Kristina Gunnarsson, Matts Wikland
OBJECTIVE: To study whether a culture medium that allows undisturbed culture supports human embryo development to the blastocyst stage equivalently to a well-established sequential media. DESIGN: Randomized, double-blinded sibling trial. SETTING: Independent in vitro fertilization (IVF) clinics. PATIENT(S): One hundred twenty-eight patients, with 1,356 zygotes randomized into two study arms. INTERVENTION(S): Embryos randomly allocated into two study arms to compare embryo development on a time-lapse system using a single-step medium or sequential media...
December 2015: Fertility and Sterility
Jingchen Chen, Andrea Streit
BACKGROUND: Enhancers are key elements to control gene expression in time and space and thus orchestrate gene function during development, homeostasis, and disease. Whole genome approaches and bioinformatic predictions have generated a tremendous pool of potential enhancers, however their spatiotemporal activity often remains to be validated in vivo. Despite recent progress in developing high throughput strategies for enhancer evaluation, these remain mainly restricted to invertebrates and in vitro cell culture...
October 2015: Developmental Dynamics: An Official Publication of the American Association of Anatomists
Ljiljana Milenkovic, Lucien E Weiss, Joshua Yoon, Theodore L Roth, YouRong S Su, Steffen J Sahl, Matthew P Scott, W E Moerner
Accumulation of the signaling protein Smoothened (Smo) in the membrane of primary cilia is an essential step in Hedgehog (Hh) signal transduction, yet the molecular mechanisms of Smo movement and localization are poorly understood. Using ultrasensitive single-molecule tracking with high spatial/temporal precision (30 nm/10 ms), we discovered that binding events disrupt the primarily diffusive movement of Smo in cilia at an array of sites near the base. The affinity of Smo for these binding sites was modulated by the Hh pathway activation state...
July 7, 2015: Proceedings of the National Academy of Sciences of the United States of America
Fatemeh Salabi, Mahmood Nazari, Wen G Cao
BACKGROUND: This study was performed to describe the basic methods to isolate and culture of primary satellite cells (PSCs) obtained from 50 to 60-day-old sheep fetuses, single cell cloning of transfected PSCs and sexing of ovine PSCs based on the ZFY/ZFX, amelogenin and high-motility-group (HMG) box sequences. RESULTS: Three-step enzymatic digestion method increased PSCs isolation from tissue and reduced the damage of cells during long time incubation with enzymes...
December 2014: Journal of Biological Research
Hong Zhang, Qinglin Han, Xianqiang Ping, Li Li, Chong Chang, Ze Chen, Yuelong Shu, Ke Xu, Bing Sun
Vaccination is the most effective way to prevent and control infection by influenza viruses, and a cell-culture-based vaccine production system is preferred as the future choice for the large-scale production of influenza vaccines. As one of the WHO-recommended cell lines for producing influenza vaccines, Vero cells do not efficiently support the growth of the current influenza A virus vaccine donor strain, the A/Puerto Rico/8/1934 (PR8) virus. In this study, a single mutation of K86R in the NS2 protein can sufficiently render the high-yielding property to the PR8 virus in Vero cells...
August 2015: Virology
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