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Blastocyst culture

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https://www.readbyqxmd.com/read/28929977/exposure-to-follicular-fluid-during-oocyte-maturation-and-oviductal-fluid-during-post-maturation-does-not-improve-in-vitro-embryo-production-in-the-horse
#1
Cécile Douet, Olivia Parodi, Nicola Antonio Martino, Giovanni Michele Lacalandra, Michele Nicassio, Fabrice Reigner, Stefan Deleuze, Maria Elena Dell'Aquila, Ghylène Goudet
Most wild equids and many domestic horse breeds are at risk of extinction, so there is an urgent need for genome resource banking. Embryos cryopreservation allows the preservation of genetics from male and female and is the fastest method to restore a breed. In the equine, embryo production in vitro would allow the production of several embryos per cycle. Intracytoplasmic sperm injection (ICSI) is used to generate horse embryos, but it requires expensive equipment and expertise in micromanipulation, and blastocyst development rates remain low...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28927798/effect-of-charcoal-dextran-stripped-fetal-bovine-serum-on-in-vitro-development-of-bovine-embryos
#2
Ayman Mesalam, Rami Kong, Imran Khan, Mmr Chowdhury, Byung-Hyun Choi, Sung Woo Kim, Kyu-Woan Cho, Jong-In Jin, Il-Keun Kong
This study investigated the ability of charcoal:dextran stripped fetal bovine serum (CDS FBS) and heat-inactivated fetal bovine serum (HI FBS) to support in vitro development of bovine embryos. The developmental ability and quality of bovine embryos were determined by assessing their cell number, lipid content, mitochondrial activity, gene expression, and cryo-tolerance. The percentage of embryos that formed a blastocyst was significantly (P<0.05) higher in medium containing CDS FBS than in medium containing HI FBS (42...
September 16, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28923420/high-doses-of-lipid-core-nanocapsules-do-not-affect-bovine-embryonic-development-in-vitro
#3
Caroline Lucas, Mariana Remião, Franciele Bruinsmann, Isadora Lopes, Morgana Borges, Ana Laura Feijó, Andrea Cristina Basso, Adriana Pohlmann, Silvia Guterres, Vinicius Campos, Fabiana Seixas, Tiago Collares
The improvement of in vitro embryo production by culture media supplementation has been a potential tool to increase blastocyst quality and development. Recently, lipid-core nanocapsules (LNC), which were developed for biomedical applications as a drug-delivery system, have demonstrated beneficial effects on in vitro embryo production studies. LNCs have a core composed of sorbitan monostearate dispersed in capric/caprylic triglyceride. Based on that, we firstly investigated if LNCs supplemented during in vitro oocyte maturation had affinity to the mineral oil placed over the top of the IVM media...
September 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28922408/the-role-of-the-pi3k-akt-signaling-pathway-in-the-developmental-competence-of-bovine-oocytes
#4
Gabriella Mamede Andrade, Juliano Coelho da Silveira, Claudia Perrini, Maite Del Collado, Samuel Gebremedhn, Dawit Tesfaye, Flávio Vieira Meirelles, Felipe Perecin
The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging "messages". We dissected bovine ovarian follicles and recovered follicular cells (FCs-granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following follicle rupture, COCs were individually selected for in vitro cultures to track the follicular cells based on oocyte competence to reach the blastocyst stage after parthenogenetic activation...
2017: PloS One
https://www.readbyqxmd.com/read/28919262/pdgfra-is-not-essential-for-the-derivation-and-maintenance-of-mouse-extraembryonic-endoderm-stem-cell-lines
#5
Jiangwei Lin, Mona Khan, Bolek Zapiec, Peter Mombaerts
Extraembryonic endoderm stem (XEN) cell lines can be derived and maintained in vitro and reflect the primitive endoderm lineage. Platelet-derived growth factor receptor alpha (PDGFRA) is thought to be essential for the derivation and maintenance of mouse XEN cell lines. Here, we have re-evaluated this requirement for PDGFRA. We derived multiple PDGFRA-deficient XEN cell lines from postimplantation and preimplantation embryos of a PDGFRA-GFP knockout strain. We also converted PDGFRA-deficient embryonic stem cell lines into XEN cell lines chemically by transient culturing with retinoic acid and Activin A...
September 8, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28913567/two-cell-embryos-are-more-sensitive-than-blastocysts-to-ampk-dependent-suppression-of-anabolism-and-stemness-by-commonly-used-fertility-drugs-a-diet-supplement-and-stress
#6
Alan Bolnick, Mohammed Abdulhasan, Brian Kilburn, Yufen Xie, Mindie Howard, Paul Andresen, Alexandra M Shamir, Jing Dai, Elizabeth E Puscheck, Eric Secor, Daniel A Rappolee
PURPOSE: This study tests whether metformin or diet supplement BR-DIM-induced AMP-activated protein kinase (AMPK) mediated effects on development are more pronounced in blastocysts or 2-cell mouse embryos. METHODS: Culture mouse zygotes to two-cell embryos and test effects after 0.5-1 h AMPK agonists' (e.g., Met, BR-DIM) exposure on AMPK-dependent ACCser79P phosphorylation and/or Oct4 by immunofluorescence. Culture morulae to blastocysts and test for increased ACCser79P, decreased Oct4 and for AMPK dependence by coculture with AMPK inhibitor compound C (CC)...
September 15, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28911212/apposition-to-endometrial-epithelial-cells-activates-mouse-blastocysts-for-implantation
#7
Peter T Ruane, Stéphane C Berneau, Rebekka Koeck, Jessica Watts, Susan J Kimber, Daniel R Brison, Melissa Westwood, John D Aplin
STUDY QUESTION: How do interactions between blastocyst-stage embryos and endometrial epithelial cells regulate the early stages of implantation in an in vitro model? SUMMARY ANSWER: Mouse blastocyst apposition with human endometrial epithelial cells initiates trophectoderm differentiation to trophoblast, which goes on to breach the endometrial epithelium. WHAT IS KNOWN ALREADY: In vitro models using mouse blastocysts and human endometrial cell lines have proven invaluable in the molecular characterisation of embryo attachment to endometrial epithelium at the onset of implantation...
September 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28889726/the-significance-of-blastocyst-culture-for-discarded-embryos-in-the-treatment-period-of-ivf-et-without-available-embryos
#8
Rong Wang, Qun Zheng, Fang Wang, Lihua Yang, Jianying He, Feifeng Wang, Libo Zou
No abstract text is available yet for this article.
September 8, 2017: Minerva Medica
https://www.readbyqxmd.com/read/28889080/reprogramming-of-rabbit-induced-pluripotent-stem-cells-toward-epiblast-and-chimeric-competency-using-kr%C3%A3-ppel-like-factors
#9
Yann Tapponnier, Marielle Afanassieff, Irène Aksoy, Maxime Aubry, Anaïs Moulin, Lucas Medjani, Wilhelm Bouchereau, Chloé Mayère, Pierre Osteil, Jazmine Nurse-Francis, Ioannis Oikonomakos, Thierry Joly, Luc Jouneau, Catherine Archilla, Barbara Schmaltz-Panneau, Nathalie Peynot, Harmonie Barasc, Alain Pinton, Jérome Lecardonnel, Elen Gocza, Nathalie Beaujean, Véronique Duranthon, Pierre Savatier
Rabbit induced pluripotent stem cells (rbiPSCs) possess the characteristic features of primed pluripotency as defined in rodents and primates. In the present study, we reprogrammed rbiPSCs using human Krüppel-like factors (KLFs) 2 and 4 and cultured them in a medium supplemented with fetal calf serum and leukemia inhibitory factor. These cells (designated rbEKA) were propagated by enzymatic dissociation for at least 30 passages, during which they maintained a normal karyotype. This new culturing protocol resulted in transcriptional and epigenetic reconfiguration, as substantiated by the expression of transcription factors and the presence of histone modifications associated with naïve pluripotency...
September 5, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28874873/the-overlaying-oil-type-influences-in-vitro-embryo-production-differences-in-composition-and-compound-transfer-into-incubation-medium-between-oils
#10
Cristina A Martinez, Alicia Nohalez, Inmaculada Parrilla, Miguel Motas, Jordi Roca, Inmaculada Romero, Diego L García-Gonza Lez, Cristina Cuello, Heriberto Rodriguez-Martinez, Emilio A Martinez, Maria A Gil
The oil overlay micro-drop system is widely used for cultures of mammalian gametes and embryos. We evaluated hereby the effects of two unaltered commercial oils- Sigma mineral oil (S-MO) and Nidoil paraffin oil (N-PO)-on in vitro embryo production (IVP) outcomes using a pig model. The results showed that while either oil apparently did not affect oocyte maturation and fertilization rates, S-MO negatively affected embryo cleavage rates, blastocyst formation rates, and, consequently, total blastocyst efficiency of the system...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28872141/derivation-of-stem-cell-lines-from-mouse-preimplantation-embryos
#11
Marta Vila-Cejudo, Elena Ibañez, Josep Santalo
Mouse embryonic stem cell (mESC) derivation is the process by which pluripotent cell lines are established from preimplantation embryos. These lines retain the ability to either self-renew or differentiate under specific conditions. Due to these properties, mESC are a useful tool in regenerative medicine, disease modeling, and tissue engineering studies. This article describes a simple protocol to obtain mESC lines with high derivation efficiencies (60-80%) by culturing blastocysts from permissive mouse strains on feeder cells in defined medium supplemented with leukemia inhibitory factor...
August 20, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28868121/melatonin-upregulates-erbb1-and-erbb4-two-primary-implantation-receptors-in-pre-implantation-mouse-embryos
#12
Ghazaleh Moshkdanian, Fatemeh Moghani-Ghoroghi, Parichehr Pasbakhsh, Seyed Noureddin Nematollahi-Mahani, Atefeh Najafi, sIraj Ragerdi Kashani
OBJECTIVES: To evaluated the effects of melatonin on early embryo competence and the expression rate of the primary implantation receptors (ErbB1 and ErbB4). MATERIALS AND METHODS: Two-cell mouse embryos were cultured in 3 groups: simple media, melatonin-treated (10(-9) M melatonin) and Luzindole-treated (10(-9) M luzindole). Then, the rate of ErbB1 and ErbB4 gene and protein expression, the level of intracellular ROS, antioxidant capacity, and also the number of cells were evaluated and compared with the fourth group in vivo developed blastocysts (control group)...
June 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28854587/prospective-study-of-automated-versus-manual-annotation-of-early-time-lapse-markers-in-the-human-preimplantation-embryo
#13
Daniel J Kaser, Leslie V Farland, Stacey A Missmer, Catherine Racowsky
STUDY QUESTION: How does automated time-lapse annotation (Eeva™) compare to manual annotation of the same video images performed by embryologists certified in measuring durations of the 2-cell (P2; time to the 3-cell minus time to the 2-cell, or t3-t2) and 3-cell (P3; time to 4-cell minus time to the 3-cell, or t4-t3) stages? SUMMARY ANSWER: Manual annotation was superior to the automated annotation provided by Eeva™ version 2.2, because manual annotation assigned a rating to a higher proportion of embryos and yielded a greater sensitivity for blastocyst prediction than automated annotation...
August 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28851957/tripolar-mitosis-and-partitioning-of-the-genome-arrests-human-preimplantation-development-in-vitro
#14
Christian S Ottolini, John Kitchen, Leoni Xanthopoulou, Tony Gordon, Michael C Summers, Alan H Handyside
Following in vitro fertilisation (IVF), only about half of normally fertilised human embryos develop beyond cleavage and morula stages to form a blastocyst in vitro. Although many human embryos are aneuploid and genomically imbalanced, often as a result of meiotic errors inherited in the oocyte, these aneuploidies persist at the blastocyst stage and the reasons for the high incidence of developmental arrest remain unknown. Here we use genome-wide SNP genotyping and meiomapping of both polar bodies to identify maternal meiotic errors and karyomapping to fingerprint the parental chromosomes in single cells from disaggregated arrested embryos and excluded cells from blastocysts...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28845753/follistatin-rescues-blastocyst-development-of-poor-quality-porcine-cumulus-oocyte-complexes-by-delaying-meiotic-resumption-with-decreased-cgmp
#15
Bo Myeong Lee, Ju Lan Chun, Ji Hye Lee, Eun Young Kim, Kang-Sun Park, Jin-Hee Lee, Bradford W Daigneault, George W Smith, Keun Jung Kim, Kyu-Tae Chang, Sang-Rae Lee, Sun-Uk Kim, Seon-A Choi, Kyung-Bon Lee, Min Kyu Kim
Mammalian oocytes resume maturation when removed from follicles and cultured in vitro. During folliculogenesis, oocytes are bathed in follicular fluid (FF), which provides an important and specialized microenvironment for oocyte competence. Follistatin (FST) is one component of FF that may play a role in oocyte maturation and embryo development. This study was conducted to examine possible effects of FST on porcine oocyte competence and embryo development. Exogenous FST in oocyte maturation medium for 22 or 44 hours did not improve nuclear maturation and had no effect on good quality cumulus-oocyte complexes (COCs)...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28844338/delayed-blastocyst-formation-or-an-extra-day-culture-increases-apoptosis-in-pig-blastocysts
#16
Tao Lin, Jae Eun Lee, Reza K Oqani, So Yeon Kim, Eun Seok Cho, Yong Dae Jeong, Jun Jong Baek, Dong Il Jin
In the present study, the timing was examined of blastocyst collection/formation or of how the duration of post-blastulation culture affected the quality and developmental competence of in vitro-produced pig parthenogenetic embryos. The earliest apoptotic signals were observed at the morula stage while the earliest cytoplasmic fragmentation was observed before the 4- to 8-cell stage of embryo development. Nuclear condensation was detected in morulae and blastocysts, but not all condensed nuclei were positive for the apoptotic signal (TUNEL staining)...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28837782/production-of-inbred-offspring-by-intracytoplasmic-sperm-injection-of-oocytes-from-juvenile-female-mice
#17
Jie Zhu, Wei Cui, Yan-Feng Dai
The aim of the present study was to determine whether the use of oocytes from juvenile female mice would improve the efficiency of intracytoplasmic sperm injection (ICSI). In the present study, 15 adult and 14 juvenile C57BL6/J female mice were superovulated, with 17.8 oocytes per mouse harvested from adults, significantly lower than the 40.2 harvested from juveniles (P<0.01). Sixty and 233 oocytes were harvested from C57BL/6J adult and juvenile mice respectively, activated in 10mM SrCl2+5μgmL-1 cytochalasin B for 5-6h and cultured in potassium simplex optimisation medium (KSOM) for 3...
August 25, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28837034/single-versus-sequential-culture-medium-which-is-better-at-improving-ongoing-pregnancy-rates-a-systematic-review-and-meta-analysis
#18
REVIEW
Felipe Dieamant, Claudia G Petersen, Ana L Mauri, Vanessa Comar, Marina Mattila, Laura D Vagnini, Adriana Renzi, Bruna Petersen, Juliana Ricci, João Batista A Oliveira, Ricardo L R Baruffi, Jose G Franco
This study aimed to evaluate if single medium is better than sequential medium at improving ongoing pregnancy rates in patients undergoing assisted reproductive technology (ART) procedures. The data featured in this meta-analysis were extracted from four randomized controlled trials yielded from a systematic search carried out on electronic databases. The primary endpoint was ongoing pregnancy rate. Secondary endpoints included clinical pregnancy and miscarriage rates. The endpoints for ongoing pregnancy rate were also analyzed based on the time at which the embryo transfers were performed: cleavage stage (day 2/3) and/or blastocyst stage (day 5/6)...
September 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28833899/non-invasive-assessment-of-culture-media-from-goat-cloned-embryos-associated-with-subjective-morphology-by-gas-chromatography-mass-spectroscopy-based-metabolomic-analysis
#19
Yan-Li Zhang, Guo-Min Zhang, Ruo-Xin Jia, Yong-Jie Wan, Hua Yang, Ling-Wei Sun, Le Han, Feng Wang
Pre-implantation embryo metabolism demonstrates distinctive characteristics associated with the development potential of embryos. We aim to determine if metabolic differences correlate with embryo morphology. In this study, gas chromatography - mass spectroscopy (GC-MS)-based metabolomics was used to assess the culture media of goat cloned embryos collected from high-quality (HQ) and low-quality (LQ) groups based on morphology. Expression levels of amino acid transport genes were further examined by quantitative real-time PCR...
August 18, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28833413/an-improved-vitrification-protocol-for-equine-immature-oocytes-resulting-in-a-first-live-foal
#20
N Ortiz-Escribano, O Bogado Pascottini, H Woelders, L Vandenberghe, C De Schauwer, J Govaere, E Van den Abbeel, T Vullers, C Ververs, K Roels, M Van De Velde, A Van Soom, K Smits
BACKGROUND: The success rate for vitrification of immature equine oocytes is low. Although vitrified-warmed oocytes are able to mature, further embryonic development appears to be compromised. OBJECTIVES: The aim of this study was to compare two vitrification protocols, and to examine the effect of the number of layers of cumulus cells surrounding the oocyte during vitrification of immature equine oocytes. STUDY DESIGN: Experimental in vitro and in vivo trials...
August 20, 2017: Equine Veterinary Journal
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