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Blastocyst culture

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https://www.readbyqxmd.com/read/28811525/totipotency-segregates-between-the-sister-blastomeres-of-two-cell-stage-mouse-embryos
#1
E Casser, S Israel, A Witten, K Schulte, S Schlatt, V Nordhoff, M Boiani
Following fertilization in mammals, it is generally accepted that totipotent cells are exclusive to the zygote and to each of the two blastomeres originating from the first mitotic division. This model of totipotency was inferred from a minority of cases in which blastomeres produced monozygotic twins in mice. Was this due to experimental limitation or biological constraint? Here we removed experimental obstacles and achieved reliable quantification of the prevalence of dual totipotency among mouse two-cell stage blastomeres...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28810691/improvement-of-implantation-potential-in-mouse-blastocysts-derived-from-ivf-by-combined-treatment-with-prolactin-epidermal-growth-factor-and-4-hydroxyestradiol
#2
Miki Takeuchi, Misato Seki, Etsuko Furukawa, Akihito Takahashi, Kyosuke Saito, Mitsuru Kobayashi, Kenji Ezoe, Emiko Fukui, Midori Yoshizawa, Hiromichi Matsumoto
STUDY QUESTION: Can supplementation of medium with prolactin (PRL), epidermal growth factor (EGF) and 4-hydroxyestradiol (4-OH-E2) prior to embryo transfer improve implantation potential in mouse blastocysts derived from IVF? SUMMARY ANSWER: Combined treatment with PRL, EGF and 4-OH-E2 improves mouse blastocyst implantation rates, while alone, each factor is ineffective. WHAT IS KNOWN ALREADY: Blastocyst dormancy during delayed implantation caused by ovariectomy is maintained by continued progesterone treatment in mice, and estrogen injection rapidly activates blastocysts to implantation-induced status in vivo...
August 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28802113/l-carnitine-enhances-oocyte-maturation-and-improves-in%C3%A2-vitro-development-of-embryos-in-dromedary-camels-camelus-dromedaries
#3
Mohamed Fathi, K H El-Shahat
The aim of the present study was to investigate the effect of L-carnitine (LC) addition during either IVM or IVC on the developmental potential of camel oocytes. In Experiment 1; camel oocytes were matured in the absence (control) or presence of different concentrations of LC (0.25 mg, 0.5 mg, 0.75 mg and 1 mg/ml) for 30 h followed by in vitro fertilization and culture up to blastocyst stage. Our results demonstrated that oocytes treated with 0.5 mg/ml LC showed higher (P < 0.05) rates of maturation (74...
August 4, 2017: Theriogenology
https://www.readbyqxmd.com/read/28800556/supplementation-of-lycopene-in-maturation-media-improves-bovine-embryo-quality-in%C3%A2-vitro
#4
M M R Chowdhury, Byung-Hyun Choi, Imran Khan, Kyeong-Lim Lee, Ayman Mesalam, Seok-Hwan Song, Lianguang Xu, Myeong-Don Joo, Fahmida Afrin, Il-Keun Kong
This study sought to modulate factors that reduce embryo quality in in vitro culture (IVC) systems. Over eight replicates, 3075 oocytes were cultured in in vitro maturation media containing various concentrations of lycopene, followed by in vitro fertilization and culture. The percentages of MII-stage oocytes, the presumptive zygotes that underwent cleavage and developed into blastocysts were significantly (P < 0.05) higher, the intracellular ROS concentrations reduced significantly (P < 0.05) in oocytes/blastocysts, TUNEL assay demonstrates reduced apoptosis and increased total cell number per blastocyst (P < 0...
August 3, 2017: Theriogenology
https://www.readbyqxmd.com/read/28799183/utilization-of-porcine-in%C3%A2-vitro-produced-parthenogenetic-embryos-for-co-transfer-with-vitrified-and-warmed-embryos
#5
Yoshiyuki Nakamura, Kazuhiro Kikuchi
The present study was conducted to evaluate the possibility of using in vitro-produced parthenogenetic (PA) embryos for co-transfer with morulae that had been collected in vivo and cryopreserved. The proportion of PA blastocysts (20.5%) was higher than that of their in vitro fertilization (IVF) counterparts (16.6%). Although there were no differences in morphology or diameter between the two groups, the number of cells in early PA blastocysts after in vitro culture for 6 days was lower than for IVF blastocysts (25...
August 10, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28795845/analysis-of-embryo-intactness-and-developmental-potential-following-slow-freezing-and-vitrification
#6
Lan Yu, Chanwei Jia, Yonglian Lan, Rui Song, Liying Zhou, Ying Li, Yu Liang, Shuyu Wang
The aim of this study was to identify the parameters that are related to intactness and developmental potential of a day 3 embryo after warming to improve the selection criteria used to cryopreserve and transfer embryos. We also sought to compare slow freezing and vitrification methods of cryopreservation and to evaluate the viability of non-intact embryos. Embryos warmed following slow freezing (n=220) or vitrification (n=522) were divided into 3 groups according to the proportion of surviving blastomeres (I<50%; II=50-99%; and III=100%)...
August 10, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28795048/evaluation-of-human-embryo-development-in-in-vitro-fertilization-and-intracytoplasmic-sperm-injection-fertilized-oocytes-a-time-lapse-study
#7
Hyung Jun Kim, Hye Jin Yoon, Jung Mi Jang, Won Don Lee, San Hyun Yoon, Jin Ho Lim
OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28795047/influence-of-the-insemination-method-on-the-outcomes-of-elective-blastocyst-culture
#8
Caizhu Wang, Guixue Feng, Bo Zhang, Jinhui Shu, Hong Zhou, Xianyou Gan, Ruoyun Lin
OBJECTIVE: The aim of this study was to explore the effects of the insemination method on the outcomes of elective blastocyst culture. METHODS: We retrospectively analyzed the outcomes of elective blastocyst culture performed between January 2011 and December 2014. RESULTS: There were 2,003 cycles of conventional in vitro fertilization (IVF) and 336 cycles of intracytoplasmic sperm injection (ICSI), including 25,652 and 4,164 embryos that underwent sequential blastocyst culture, respectively...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28795046/the-effects-of-different-types-of-media-on-in-vitro-maturation-outcomes-of-human-germinal-vesicle-oocytes-retrieved-in-intracytoplasmic-sperm-injection-cycles
#9
Farzaneh Fesahat, Razieh Dehghani Firouzabadi, Azita Faramarzi, Mohammad Ali Khalili
OBJECTIVE: Optimizing in vitro maturation (IVM) media to achieve better outcomes has been a matter of interest in recent years. The aim of this prospective clinical trial was to investigate the effects of different media on the IVM outcomes of immature oocytes at the germinal vesicle (GV) stage. METHODS: A total of 400 immature oocytes at the GV stage with normal morphology were retrieved from 320 infertile women aged 31±4.63 years during stimulated intracytoplasmic sperm injection (ICSI) cycles...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28794899/an-extended-model-for-culture-dependent-heterogenous-gene-expression-and-proliferation-dynamics-in-mouse-embryonic-stem-cells
#10
Simon Godwin, Daniel Ward, Elisa Pedone, Martin Homer, Alexander G Fletcher, Lucia Marucci
During development, pluripotency is a transient state describing a cell's ability to give rise to all three germ layers and germline. Recent studies have shown that, in vitro, pluripotency is highly dynamic: exogenous stimuli provided to cultures of mouse embryonic stem cells, isolated from pre-implantation blastocysts, significantly affect the spectrum of pluripotency. 2i/LIF, a recently defined serum-free medium, forces mouse embryonic stem cells into a ground-state of pluripotency, while serum/LIF cultures promote the co-existence of ground-like and primed-like mouse embryonic stem cell subpopulations...
2017: NPJ Systems Biology and Applications
https://www.readbyqxmd.com/read/28782881/from-peptide-masses-to-pregnancy-maintenance-a-comprehensive-proteomic-analysis-of-the-early-equine-embryo-secretome-blastocoel-fluid-and-capsule
#11
Aleona Swegen, Christopher Gerald Grupen, Zamira Gibb, Mark Baker, Marta de Ruijter-Villani, Nathan Druery Smith, Tom A E Stout, R John Aitken
Early pregnancy in the mare is a poorly understood, high risk period during which the embryo communicates its presence to the maternal endometrium. Remarkably, the maternal recognition of pregnancy signal is unknown in the horse. This study aimed to profile the proteins secreted by equine blastocysts into their immediate environment, along with proteins contained in the blastocoel and within the acellular embryo capsule. Embryos were recovered on day 8 after ovulation and cultured for 48 hours. Secretomes of day 9 and day 10 embryos were analyzed by LC-MS/MS and supported by analysis of blastocoel fluid and embryo capsule...
August 7, 2017: Proteomics
https://www.readbyqxmd.com/read/28776828/the-quality-after-culture-in%C3%A2-vitro-or-in%C3%A2-vivo-of-porcine-oocytes-matured-and-fertilized-in%C3%A2-vitro-and-their-ability-to-develop-to-term
#12
Yoshiyuki Nakamura, Sigeyuki Tajima, Kazuhiro Kikuchi
The quality of porcine blastocysts produced in vitro is poor in comparison with those that develop in vivo. We examined the quality of in vitro-matured and fertilized (IVM/IVF) oocytes, their abilities to develop to blastocysts under in vivo and in vitro conditions, and the potential of the embryos to develop to term after transfer. IVM/IVF oocytes were either transferred and the embryos recovered on Days 5 and 6 (100% and 87.5%, respectively) ('ET-vivo' embryos), or cultured in vitro for 5 or 6 days ('IVC' embryos)...
August 4, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28764817/the-rpmi-1640-vitamin-mixture-promotes-bovine-blastocyst-development-in-vitro-and-downregulates-gene-expression-of-txnip-with-epigenetic-modification-of-associated-histones
#13
S Ikeda, M Sugimoto, S Kume
Diverse environmental conditions surrounding preimplantation embryos, including available nutrients, affect their metabolism and development in both short- and long-term manner. Thioredoxin-interacting protein (TXNIP) is a possible marker for preimplantation stress that is implicated in in vitro fertilization- (IVF) induced long-term DOHaD effects. B vitamins, as participants in one-carbon metabolism, may affect preimplantation embryos by epigenetic alterations of metabolically and developmentally important genes...
August 2, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28763725/peroxidized-mineral-oil-increases-the-oxidant-status-of-culture-media-and-inhibits-in%C3%A2-vitro-porcine-embryo-development
#14
C A Martinez, A Nohalez, J J Ceron, C P Rubio, J Roca, C Cuello, H Rodriguez-Martinez, E A Martinez, M A Gil
The use of oils with undetected alterations is a long-recognized problem for in vitro embryo production systems. Since peroxides in oils have been associated with reduced embryo production outcomes, our goals were (1) to evaluate the effects of a batch of mineral oil (MO) that was suspected to be altered on the in vitro production of pig embryos and (2) to determine oil peroxide values throughout culture and the transfer of oxidant agents from oil to culture media. Sunflower oil, which has a completely different chemical composition than MO but a higher oxidative status, and unaltered MO were used as controls...
July 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/28763675/transcriptional-and-epigenetic-control-in-mouse-pluripotency-lessons-from-in-vivo-and-in-vitro-studies
#15
REVIEW
Ehsan Habibi, Hendrik G Stunnenberg
Pluripotent cells were first derived from mouse blastocysts several decades ago. Since then, our knowledge of the molecular events that occur in the pre-implantation embryo has been vastly progressing. The emergence of epigenetics has revolutionized stem cell and developmental biology and further deepened our understanding of the underlying molecular mechanisms controlling the early embryo development. In particular, the emergence of massive parallel sequencing technologies has opened new avenues and became indispensable tools in modern biology...
July 29, 2017: Current Opinion in Genetics & Development
https://www.readbyqxmd.com/read/28758255/leucemia-inhibitory-factor-investigating-the-time-dependent-effect-on-viability-of-vitrified-bovine-embryos
#16
A Kocyigit, M Cevik
Leucemia inhibitory factor (LIF) is involved in various reproductive processes, including sperm development, regulation of ovulation, as well as blastocyst formation, hatching and implantation in embryos. Moreover, LIF has also been shown significantly to enhance the blastocyst formation rates of bovine embryos, a finding that remains controversial. Our purpose was to investigate time-dependent effect of LIF on bovine embryo culture, especially in terms of addition timing. Presumptive zygotes were cultured in five different groups...
July 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28756654/adverse-effect-of-superovulation-treatment-on-maturation-function-and-ultrastructural-integrity-of-murine-oocytes
#17
Myungook Lee, Jong Il Ahn, Ah Ran Lee, Dong Woo Ko, Woo Sub Yang, Gene Lee, Ji Yeon Ahn, Jeong Mook Lim
Regular monitoring on experimental animal management found the fluctuation of ART outcome, which showed a necessity to explore whether superovulation treatment is responsible for such unexpected outcome. This study was subsequently conducted to examine whether superovulation treatment can preserve ultrastructural integrity and developmental competence of oocytes following oocyte activation and embryo culture. A randomized study using mouse model was designed and in vitro development (experiment 1), ultrastructural morphology (experiment 2) and functional integrity of the oocytes (experiment 3) retrieved after PMSG/hCG injection (superovulation group) or not (natural ovulation; control group) were evaluated...
July 31, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28754923/tauroursodeoxycholic-acid-enhances-the-development-of-porcine-embryos-derived-from-in-vitro-matured-oocytes-and-evaporatively-dried-spermatozoa
#18
Xiao-Xia Li, Yun-Fei Diao, Hai-Jun Wei, Shi-Yong Wang, Xin-Yan Cao, Yu-Fei Zhang, Tong Chang, Dan-Li Li, Min Kyu Kim, Baozeng Xu
Evaporative drying (ED) is an alternative technique for long-term preservation of mammalian sperm, which does not require liquid nitrogen or freeze-drying equipment, but offers advantages for storage and shipping at ambient temperature and low cost. However, the development of zygotes generated from these sperms was poor. Here, we demonstrated that the supplementation of tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, during embryo culture improved the developmental competency of embryos derived from in vitro matured pig oocytes injected intracytoplasmically with boar ED spermatozoa by reducing the production of reactive oxygen species, the DNA degradation and fragmentation, and the expression of apoptosis-related gene Bax and Bak, and by increasing the transcription of anti-apoptosis gene Bcl-XL and Bcl-2...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28736154/retinoic-acid-induces-differentiation-of-buffalo-bubalus-bubalis-embryonic-stem-cells-into-germ-cells
#19
Syed Mohmad Shah, Suresh Kumar Singla, Prabhat Palta, Radhey Sham Manik, Manmohan Singh Chauhan
Development of precise and reproducible culture system for in vitro differentiation of embryonic stem (ES) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (PGCs), but in finally treating the catastrophic infertility. Taking into account the significant role of retinoic acid (RA) during in vivo gametogenesis, we designed the present study to investigate the effects of its stimulation on directing the differentiation of ES cells into germ cells...
July 19, 2017: Gene
https://www.readbyqxmd.com/read/28735105/inherent-inferior-quality-of-follicular-fluid-in-repeat-breeder-heifers-as-evidenced-by-low-rates-of-in%C3%A2-vitro-production-of-bovine-embryos
#20
M Kafi, M Azari, O Chashnigir, S Gharibzadeh, Z Aghabozorgi, A Asaadi, M R Divar
The aims of the present study were to determine the effect of follicular fluid obtained from the ovulatory follicle of repeat breeder heifers on in vitro oocyte maturation (Experiment 1), fertilization (Experiment 2) and production of bovine embryos (Experiment 3). Holstein virgin heifers (VH, n = 5) with normal fertility or repeat breeder syndrome (RBH, n = 5) were used in the present study. Follicular fluid of VH and RBH was aspirated from ovulatory follicles and used as maturation medium. Bovine oocytes were aspirated from follicles of slaughterhouse ovaries and randomly allocated in three groups; in Group 1, oocytes cultured in TCM-199 supplemented with 10% heat-treated fetal calf serum and hormones (5 IU/mL hCG plus 0...
July 16, 2017: Theriogenology
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