keyword
MENU ▼
Read by QxMD icon Read
search

Blastocyst culture

keyword
https://www.readbyqxmd.com/read/29149327/focused-time-lapse-analysis-reveals-novel-aspects-of-human-fertilization-and-suggests-new-parameters-of-embryo-viability
#1
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
November 15, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29142449/single-blastocyst-transfer-the-key-to-reduce-multiple-pregnancy-rates-without-compromising-the-live-birth-rate
#2
Uma M Sundhararaj, Monali V Madne, Reeta Biliangady, Sumana Gurunath, Ambika G Swamy, Indu S T Gopal
Background: Historically, to achieve higher pregnancy rates, multiple embryos were transferred after an in-vitro fertilisation (IVF). However, this practice is being reassessed, because it leads to multiple pregnancies that is known to cause adverse maternal and fetal outcomes. Aim: To compare the pregnancy outcomes in fresh IVF or intracytoplasmic sperm injection (ICSI) cycles among women undergoing elective single blastocyst transfer (eSBT) vs. those undergoing double blastocyst transfer (DBT)...
July 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29136144/antioxidants-improve-ivf-outcome-and-subsequent-embryo-development-in-the-mouse
#3
T Truong, D K Gardner
STUDY QUESTION: What is the effect of a combination of three antioxidants (Acetyl-L-Carnitine, N-Acetyl-L-Cysteine and α-Lipoic Acid), present in IVF medium during mouse oocyte and sperm collection, on fertilization and subsequent IVF embryo development? SUMMARY ANSWER: A combination of antioxidants resulted in faster developmental times from the 2-cell stage through to expanded blastocyst stage, accompanied by a significant increase in blastocyst cell number and a reduction of intracellular hydrogen peroxide (H2O2) levels...
November 10, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29132036/holding-immature-bovine-oocytes-in-a-commercial-embryo-holding-medium-high-developmental-competence-for-up-to-10%C3%A2-h-at-room-temperature
#4
Osvaldo Bogado Pascottini, Maaike Catteeuw, Ann Van Soom, Geert Opsomer
Bovine in vitro embryo production (IVP) following Ovum Pick Up (OPU) is all too often hampered by a large time gap between the harvest of oocytes of the first and last OPU session of the day. Immediately after retrieval, oocyte maturation is initiated, resulting in oocytes maturing at different time points which necessitates laborious scheduling of the IVP process. In this study, the potential of a commercial embryo holding medium (EHM; Syngro, Bioniche Inc.) to hold immature bovine oocytes was validated. We assessed the effect of holding time and temperature on (1) oocytes' maturation; (2) blastocyst development and quality at day 8 post insemination; and (3) blastocyst yield in small groups of oocytes/zygotes simulating OPU settings...
November 4, 2017: Theriogenology
https://www.readbyqxmd.com/read/29124461/improvement-of-pregnancy-outcome-by-extending-embryo-culture-in-ivf-et-during-clinical-application
#5
Xiaopeng Zhao, Binbin Ma, Shaokang Mo, Lu Ma, Fei Chang, Liyuan Zhang, Fang Xu, Ling Wang
PURPOSE: The purpose of this study is to investigate the application value of the extended embryo culture for 7-8 h in day 3 morning during IVF-ET process. METHODS: Embryos were retrospectively assessed during 08:00-09:00 on the morning of day 3 in the control group, and were assessed once again at 16:00 in the afternoon in the extended culture (EC) group. The embryos with good developmental potential were preferentially selected to transfer. The cumulative pregnancy outcomes were analyzed in one oocyte retrieval cycle...
November 9, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29123210/plasticity-of-the-inner-cell-mass-in-mouse-blastocyst-is-restricted-by-the-activity-of-fgf-mapk-pathway
#6
M Wigger, K Kisielewska, K Filimonow, B Plusa, M Maleszewski, A Suwińska
In order to ensure successful development, cells of the early mammalian embryo must differentiate to either trophectoderm (TE) or inner cell mass (ICM), followed by epiblast (EPI) or primitive endoderm (PE) specification within the ICM. Here, we deciphered the mechanism that assures the correct order of these sequential cell fate decisions. We revealed that TE-deprived ICMs derived from 32-cell blastocysts are still able to reconstruct TE during in vitro culture, confirming totipotency of ICM cells at this stage...
November 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29119699/effect-of-follistatin-on-pre-implantational-development-of-pig-parthenogenetic-embryos
#7
Yunsheng Li, Xing Liu, Zhen Chen, Dandan Song, Jie Yang, Xiaoyuan Zuo, Zubing Cao, Ya Liu, Yunhai Zhang
The present study was designed to explore effects of follistatin (FST) on pre-implantational development of parthenogenetically activated embryos (PAEs) in pigs. First, we investigated the FST messenger RNA expression level and dynamic FST protein expression patterns in porcine oocytes and PAEs. Then, PAEs were placed in embryo culture medium supplemented with 10 ng/mL of FST-288, FST-300, and FST-315. Next, PAEs were cultured with 0, 1, 10 and 100 ng/mL of FST-315 protein throughout the in vitro culture (IVC) duration...
November 9, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29113834/aflatoxin-b1-impairs-sperm-quality-and-fertilization-competence
#8
A Komsky-Elbaz, M Saktsier, Z Roth
Aflatoxins are poisonous byproducts of the soilborne fungus Aspergillus, involved in the decomposition of plant materials. Aflatoxins can be found in various food products, such as maize, sorghum, millet, rice and wheat. AFB1 is the most toxic of these, classified as a carcinogen and mutagen for both humans and animals. AFB1 has been detected in human cord blood and placenta; however, its toxic effect on sperm is less known. The current study examines sperm responses associated with AFB1 exposure. These included acrosome integrity and function, mitochondrial polarity, DNA fragmentation, fertilization competence and early embryonic development...
November 4, 2017: Toxicology
https://www.readbyqxmd.com/read/29110378/generation-of-embryonic-stem-like-cells-from-in-vivo-derived-porcine-blastocysts-at-a-low-concentration-of-basic-fibroblast-growth-factor
#9
H-J Cha, J I Yun, N R Han, H-Y Kim, S Baek, S-H Lee, J Lee, E Lee, C-K Park, S T Lee
Although basic fibroblast growth factor (bFGF) is an essential factor supporting the maintenance of porcine embryonic stem (ES) cell self-renewal and pluripotency, its high cost has limited previous studies, and the development of a low-cost culture system is required. For these systems, in vivo blastocysts were progressively cultured under various conditions consisting of different culture mediums and/or different feeder cell numbers at a low concentration of bFGF. As the results, the sequential culture of in vivo-derived porcine blastocysts on 5...
November 6, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29108044/consequences-of-exposure-of-embryos-produced-in-vitro-in-a-serum-containing-medium-to-dickkopf-related-protein-1-and-colony-stimulating-factor-2-on-blastocyst-yield-pregnancy-rate-and-birth-weight
#10
P Tríbulo, B H Bernal Ballesteros, A Ruiz, A Tríbulo, R J Tríbulo, H E Tríbulo, G A Bo, P J Hansen
Embryokines are molecules secreted by the mother that regulate embryonic development. Among these molecules in cattle are colony stimulating factor 2 (CSF2) and dickkopf-related protein 1 (DKK1). Here, we evaluated actions of CSF2 and DKK1 alone or in combination on characteristics of embryos produced in vitro in the presence of serum. A total of 70 beef cows from 4 farms were subjected to oocyte retrieval on 1 to 4 occasions. Within each farm, donors were randomly allocated to 1 of 4 treatment groups (vehicle, CSF2, DKK1, CSF2 + DKK1)...
October 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/29107591/naked-mole-rat-induced-pluripotent-stem-cells-and-their-contribution-to-interspecific-chimera
#11
Sang-Goo Lee, Aleksei E Mikhalchenko, Sun Hee Yim, Alexei V Lobanov, Jin-Kyu Park, Kwang-Hwan Choi, Roderick T Bronson, Chang-Kyu Lee, Thomas J Park, Vadim N Gladyshev
Naked mole rats (NMRs) are exceptionally long-lived, cancer-resistant rodents. Identifying the defining characteristics of these traits may shed light on aging and cancer mechanisms. Here, we report the generation of induced pluripotent stem cells (iPSCs) from NMR fibroblasts and their contribution to mouse-NMR chimeric embryos. Efficient reprogramming could be observed under N2B27+2i conditions. The iPSCs displayed a characteristic morphology, expressed pluripotent markers, formed embryoid bodies, and showed typical differentiation patterns...
November 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29106766/antioxidant-responses-and-deregulation-of-epigenetic-writers-and-erasers-link-oxidative-stress-and-dna-methylation-in-bovine-blastocysts
#12
Monalisa M Bomfim, Gabriella M Andrade, Maite Del Collado, Juliano R Sangalli, Patrícia K Fontes, Marcelo F G Nogueira, Flávio V Meirelles, Juliano C da Silveira, Felipe Perecin
Early mammalian embryos derived from in vitro fertilization are exposed to conditions distinct from the native oviduct-uterine environment, including atmospheric oxygen that promotes cellular oxidative stress and alters gene expression. High oxygen partial pressure during embryo development is associated with low pregnancy rates and increased embryonic apoptosis. We investigated how bovine embryos responded to high (20% O2 ) or low (5% O2 ) oxygen partial pressure during in vitro culture, evaluating levels of reactive oxygen species (ROS) as well as changes in the expression of oxidative stress- and epigenetic-related transcripts and miRNAs in blastocysts...
November 6, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29105396/effects-of-re-vitrification-of-mouse-morula-and-early-blastocyst-stages-on-apoptotic-gene-expression-and-developmental-potential
#13
Nasrin Majidi Gharenaz, Mansoureh Movahedin, Zohreh Mazaheri
OBJECTIVES: Re-vitrification of embryos immediately after thawing or after a culture period could be used to preserve the extra embryos after embryo transfer. This study aims to clarify the effect of re-vitrification on Bax and Bcl-2 gene expressions of compact and early blastocyst stage embryos. MATERIALS AND METHODS: This experimental study was performed on mouse embryos. We collected 8-cell stage embryos (n=400) from female mature mice, 60-62 hoursafter injection of human chorionic gonadotropin (hCG)...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29105395/the-effect-of-vitrification-on-expression-and-histone-marks-of-igf2-and-oct4-in-blastocysts-cultured-from-two-cell-mouse-embryos
#14
Maryam Jahangiri, Maryam Shahhoseini, Bahar Movaghar
OBJECTIVES: Vitrification is increasingly used in assisted reproductive technology (ART) laboratories worldwide. In this study the effect of vitrification on the expression and modifications of H3 histones of Igf2 and Oct4 was investigated in blastocysts cultured from vitrified and non-vitrified two-cell embryos. MATERIALS AND METHODS: In this experimental study, two-cell embryos were cultured in KSOM medium to reach the blastocyst stage. Expression of Igf2 and Oct4 and modifications of H3 histones in regulatory regions of both genes were compared with in vivo blastocysts, which comprise the control group...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29105394/melatonin-pretreated-blastocysts-along-with-calcitonin-administration-improved-implantation-by-upregulation-of-heparin-binding-epidermal-growth-factor-expression-in-murine-endometrium
#15
Fatemeh Moghani-Ghoroghi, Ghazaleh Moshkdanian, Mojtaba Sehat, Seyed Noureddin Nematollahi-Mahani, Iraj Ragerdi-Kashani, Parichehr Pasbakhsh
OBJECTIVES: Implantation failure is an obstacle in assisted reproduction techniques (ART). Calcitonin is a molecules involved in uterine receptivity and embryo implantation. Melatonin can promote embryo quality and improve implantation. This study examines the effect of pretreatment of blastocysts with melatonin and calcitonin on heparin binding-epidermal growth factor (HB-EGF) expression in murine endometrium. MATERIALS AND METHODS: In this experimental study, we collected 2-cell embryos from the oviducts of 1...
January 2018: Cell Journal
https://www.readbyqxmd.com/read/29102427/effect-of-repeated-cryopreservation-on-human-embryo-developmental-potential
#16
Xiaoying Zheng, Yuan Chen, Jie Yan, Yuqi Wu, Xinjie Zhuang, Shengli Lin, Jinliang Zhu, Ying Lian, Jie Qiao, Ping Liu
Repeated cryopreservation of surplus embryos from frozen-thawed cycles should occasionally be considered. The purpose of this retrospective cohort study was to evaluate the pregnancy and perinatal outcome of repeated cryopreservation by vitrification of human blastocysts derived from slowly frozen-thawed day 3 embryos. In total, 571 vitrified-warmed blastocyst transfer cycles were investigated. The vitrified-warmed blastocysts were derived from slowly frozen-thawed cleavage embryos (twice-cryopreserved group) or fresh embryos (control group) cultured to the blastocyst stage...
August 24, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29099865/relief-of-endoplasmic-reticulum-stress-enhances-dna-damage-repair-and-improves-development-of-pre-implantation-embryos
#17
Naomi Dicks, Rodrigo C Bohrer, Karina Gutierrez, Marek Michalak, Luis B Agellon, Vilceu Bordignon
Early-cleaving embryos are known to have better capacity to reach the blastocyst stage and produce better quality embryos compared to late-cleaving embryos. To investigate the significance of endoplasmic reticulum (ER) stress on early embryo cleavage kinetics and development, porcine embryos produced in vitro were separated into early- and late-cleaving groups and then cultured in the absence or presence of the ER stress inhibitor tauroursodeoxycholic acid (TUDCA). Developing embryos were collected at days 3 to 7 of culture for assessment of ER stress status, incidence of DNA double-strand breaks (DSBs), development and total cell number...
2017: PloS One
https://www.readbyqxmd.com/read/29096276/influence-of-resveratrol-pretreatment-on-thawed-bovine-embryo-quality-and-mitochondrial-dna-copy-number
#18
T Hayashi, S Ueda, M Mori, T Baba, T Abe, H Iwata
This study addresses the hypothesis that SIRT1 upregulation prior to cryopreservation helps in recuperation from cryoinjury and improves the embryo quality. Bovine blastocysts were produced in vitro, and at Day 6 or 7 after fertilization, the blastocysts were cultured with 0 or 1 μM resveratrol for 6 or 24 h. This short duration of resveratrol treatment did not affect the embryo development or the grade of the blastocysts. However, both the durations of resveratrol treatment (6 h or 24 h) significantly increased the expression levels of SIRT1...
October 22, 2017: Theriogenology
https://www.readbyqxmd.com/read/29096269/quality-of-common-marmoset-callithrix-jacchus-oocytes-collected-after-ovarian-stimulation
#19
Akifumi Kanda, Asako Nobukiyo, Miyuki Yoshioka, Teruhiko Hatakeyama, Yusuke Sotomaru
The common marmoset (Callithrix jacchus) is an experimental animal that is considered suitable for the creation of next-generation human disease models. It has recently been used in the reproductive technology field. Oocytes can be effectively collected from female marmosets via ovarian stimulation with injections of follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG). The oocytes, collected about 28 h after the hCG injection, include both premature oocytes and postmature (in vivo matured; IVO) oocytes, and the premature oocytes can be matured by in vitro culture (in vitro matured; IVM)...
October 22, 2017: Theriogenology
https://www.readbyqxmd.com/read/29095916/transcriptomic-difference-in-bovine-blastocysts-following-vitrification-and-slow-freezing-at-morula-stage
#20
Alisha Gupta, Jaswant Singh, Isabelle Dufort, Claude Robert, Fernanda Caminha Faustino Dias, Muhammad Anzar
Cryopreservation is known for its marked deleterious effects on embryonic health. Bovine compact morulae were vitrified or slow-frozen, and post-warm morulae were cultured to the expanded blastocyst stage. Blastocysts developed from vitrified and slow-frozen morulae were subjected to microarray analysis and compared with blastocysts developed from unfrozen control morulae for differential gene expression. Morula to blastocyst conversion rate was higher (P < 0.05) in control (72%) and vitrified (77%) than in slow-frozen (34%) morulae...
2017: PloS One
keyword
keyword
95878
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"