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Blastocyst culture

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https://www.readbyqxmd.com/read/28215677/pyridoxine-supplementation-during-oocyte-maturation-improves-the-development-and-quality-of-bovine-preimplantation-embryos
#1
Mansour Aboelenain, Ahmed Zaky Balboula, Manabu Kawahara, Abd El-Monem Montaser, Samy Moawad Zaabel, Sung-Woo Kim, Masashi Nagano, Masashi Takahashi
Recently, inhibition of cathepsin B (CTSB) activity during in vitro maturation (IVM) and culture (IVC) improved the developmental competence and quality of bovine oocytes and embryos. E-64 is a widely used inhibitor to inhibit CTSB activity, however, E-64 inhibits not only CTSB activity but also the activities of other proteases including cathepsin L (CTSL), papain, calpain, and trypsin. Pyridoxine, the catalytically active form of vitamin B6, plays a crucial role in several cellular processes and has the ability to inhibit CTSB activity...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28215673/supplement-of-cilostamide-in-growth-medium-improves-oocyte-maturation-and-developmental-competence-of-embryos-derived-from-small-antral-follicles-in-pigs
#2
Hanna Lee, Fazle Elahi, Joohyeong Lee, Seung Tae Lee, Sang-Hwan Hyun, Eunsong Lee
This study was conducted to evaluate the effects of cyclic AMP (cAMP) modulator cilostamide (CIL) and forskolin (FSK) treatment during in vitro growth (IVG) on growth, maturation, and embryonic development of cumulus-oocyte complexes (COCs) derived from small antral follicles < 3 mm in diameter (SAFCOCs). SAFCOCs were untreated (control) or treated with 20 μM CIL and/or 50 μM FSK for 2 days for IVG. Next, IVG oocytes were cultured for maturation and then induced for parthenogenesis (PA) or used as recipient ooplasts for somatic cell nuclear transfer (SCNT)...
March 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/28196411/effects-of-culture-dimensions-on-maintenance-of-porcine-inner-cell-mass-derived-cell-self-renewal
#3
Song Baek, Na Rae Han, Jung Im Yun, Jae Yeon Hwang, Minseok Kim, Choon Keun Park, Eunsong Lee, Seung Tae Lee
Despite the fact that porcine embryonic stem cells (ESCs) are a practical study tool, in vitro long-term maintenance of these cells is difficult in a two-dimensional (2D) microenvironment using cellular niche or extracellular matrix proteins. However, a three-dimensional (3D) microenvironment, similar to that enclosing the inner cell mass of the blastocyst, may improve in vitro maintenance of self-renewal. Accordingly, as a first step toward constructing a 3D microenvironment optimized to maintain porcine ESC self-renewal, we investigated different culture dimensions for porcine ICM-derived cells to enhance the maintenance of self-renewal...
February 15, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28193316/polydatin-improves-the-developmental-competence-of-bovine-embryos-in-vitro-via-induction-of-sirtuin-1-sirt1
#4
Imran Khan, Sung Woo Kim, Kyung-Lim Lee, Seok-Hwan Song, Ayman Mesalam, M M R Chowdhury, Zia Uddin, Ki Hun Park, Il-Keun Kong
The aim of the present study was to investigate the beneficial effect of polydatin (PD), the glycoside form of resveratrol, on embryo development in vitro. Oocytes were aspirated from ovaries of Korean Hanwoo cows and cultured until Day 8 in a humidified atmosphere of 5% CO2 in air at 38.5°C. Protein and gene expression levels were determined through confocal microscopy and reverse transcription-polymerase chain reaction respectively, whereas the number of total and apoptotic cells in Day 8 blastocysts was determined using Hoechst 33342 staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling...
February 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28181558/an-air-liquid-interphase-approach-for-modeling-the-early-embryo-maternal-contact-zone
#5
S Chen, S E Palma-Vera, M Langhammer, S P Galuska, B C Braun, E Krause, A Lucas-Hahn, J Schoen
We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions.
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28176010/clinical-potentials-of-human-pluripotent-stem-cells
#6
REVIEW
Cristina Mora, Marialaura Serzanti, Antonella Consiglio, Maurizio Memo, Patrizia Dell'Era
Aging, injuries, and diseases can be considered as the result of malfunctioning or damaged cells. Regenerative medicine aims to restore tissue homeostasis by repairing or replacing cells, tissues, or damaged organs, by linking and combining different disciplines including engineering, technology, biology, and medicine. To pursue these goals, the discipline is taking advantage of pluripotent stem cells (PSCs), a peculiar type of cell possessing the ability to differentiate into every cell type of the body. Human PSCs can be isolated from the blastocysts and maintained in culture indefinitely, giving rise to the so-called embryonic stem cells (ESCs)...
February 8, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28169188/clinical-outcomes-after-ivf-or-icsi-using-human-blastocysts-derived-from-oocytes-containing-aggregates-of-smooth-endoplasmic-reticulum
#7
Fumiaki Itoi, Yukiko Asano, Masashi Shimizu, Rika Nagai, Kanako Saitou, Hiroyuki Honnma, Yasutaka Murata
In this study the clinical and neo-natal outcomes after transfer of blastocysts derived from oocytes containing aggregates of smooth endoplasmic reticulum (SER) were compared between IVF and intracytoplasmic sperm injection (ICSI) cycles. Clinical and neo-natal outcomes of blastocysts in cycles with at least one SER metaphase II oocyte (SER + MII; SER + cycles) did not significantly differ between the two insemination methods. When SER + MII were cultured to day 5/6, fertilization, embryo cleavage and blastocyst rates were not significantly different between IVF and ICSI cycles...
January 25, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28166968/interspecies-somatic-cell-nuclear-transfer-in-asiatic-cheetah-using-nuclei-derived-from-post-mortem-frozen-tissue-in-absence-of-cryo-protectant-and-in%C3%A2-vitro-matured-domestic-cat-oocytes
#8
F Moulavi, S M Hosseini, N Tanhaie-Vash, S Ostadhosseini, S H Hosseini, M Hajinasrollah, M H Asghari, H Gourabi, A Shahverdi, A D Vosough, M H Nasr-Esfahani
Recent accomplishments in the field of somatic cell nuclear transfer (SCNT) hold tremendous promise to prevent rapid loss of animal genetic resources using ex situ conservation technology. Most of SCNT studies use viable cells for nuclear transfer into recipient oocytes. However, preparation of live cells in extreme circumstances, in which post-mortem material of endangered/rare animals is improperly retained frozen, is difficult, if not impossible. This study investigated the possibility of interspecies-SCNT (iSCNT) in Asiatic cheetah (Acinonyx jubatus venaticus), a critically endangered subspecies, using nuclei derived from frozen tissue in absence of cryo-protectant at -20 °C and in vitro matured domestic cat oocytes...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28166962/expression-and-localization-of-artemin-in-the-bovine-uterus-and-embryos
#9
E Gómez, D Martin, S Carrocera, M J Sánchez-Calabuig, A Gutierrez-Adán, M Alonso-Guervos, N Peynot, C Giraud-Delville, O Sandra, V Duranthon, M Muñoz
Artemin a member of the glial cell line-derived neurotrophic factor (GDNF) family is present in mice and human preimplantation embryos, and reproductive tract, during early pregnancy promoting embryo development in vitro. The presence of artemin in cattle embryos and reproductive tract, however, is unknown. In the present work we identified for first time artemin in bovine uterine fluid (UF) (Western blot), endometrium (RT-PCR, Western blot and immunohistochemistry) and embryos (RT-PCR and immunohistochemistry) during early preimplantation development...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28161857/on-developing-a-thesis-for-reproductive-endocrinology-and-infertility-fellowship-a-case-study-of-ultra-low-2-oxygen-tension-for-extended-culture-of-human-embryos
#10
REVIEW
Daniel J Kaser
Fellows in Reproductive Endocrinology and Infertility training are expected to complete 18 months of clinical, basic, or epidemiological research. The goal of this research is not only to provide the basis for the thesis section of the oral board exam but also to spark interest in reproductive medicine research and to provide the next generation of physician-scientists with a foundational experience in research design and implementation. Incoming fellows often have varying degrees of training in research methodology and, likewise, different career goals...
February 4, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28161437/tissue-organization-alters-gene-expression-in-equine-induced-trophectoderm-cells
#11
Brad M Reinholt, Jennifer S Bradley, Robert D Jacobs, Alan D Ealy, Sally E Johnson
Rapid morphological and gene expression changes occur during the early formation of a mammalian blastocyst. Critical to successful retention of the blastocyst and pregnancy is a functional trophectoderm (TE) that supplies the developing embryo with paracrine factors and hormones. The contribution of TE conformational changes to gene expression was examined in equine induced trophoblast (iTr) cells. Equine iTr cells were cultured as monolayers or in suspension to form spheres. The spheres are hollow and structurally reminiscent of native equine blastocysts...
February 1, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28153493/cyp2c76-deficiency-is-embryonic-lethal-in-cynomolgus-macaques-the-potential-role-of-cyp2c76-in-early-embryogenesis
#12
Shuzo Koyama, Koji Fukuda, Sho Watanabe, Akinori Matsushita, Hideaki Tsuchiya, Nahoko Fujinami, Sakae Kohara, Norie Murayama, Masashi Nagano, Hiroshi Yamazaki, Koichiro Fukuzaki, Yasuhiro Uno, Yoshihiko Hosoi
Cynomolgus macaques are an important primate species for drug metabolism studies; however cynomolgus CYP2C76, an important drug-metabolizing enzyme, accounts for drug metabolism differences to humans, so that CYP2C76-null animals might show drug-metabolizing properties more similar to humans. In this study, attempts were made to produce CYP2C76-null animals by assisted reproduction technology. Oocytes and sperm collected from the heterozygotes for the null allele (c.449TG > A) were subjected to intracytoplasmic sperm injection, and the embryos produced were cultured in vitro through the blastocyst stage...
October 27, 2016: Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/28137339/satellite-dna-methylation-status-and-expression-of-selected-genes-in-bos-indicus-blastocysts-produced-in-vivo-and-in-vitro
#13
R Urrego, S M Bernal-Ulloa, N A Chavarría, E Herrera-Puerta, A Lucas-Hahn, D Herrmann, S Winkler, D Pache, H Niemann, N Rodriguez-Osorio
Bovine embryos produced in vivo and in vitro differ with respect to molecular profiles, including epigenetic marks and gene expression profiles. This study investigated the CpG methylation status in bovine testis satellite I (BTS) and Bos taurus alpha satellite I (BTαS) DNA sequences, and concomitantly the relative abundance of transcripts, critically involved in DNA methylation (DNMT1 and DNMT3A), growth and development (IGF2R) and pluripotency (POU5F1) in Bos indicus embryos produced in vitro or in vivo...
January 31, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28134613/dna-methylation-and-gene-expression-changes-derived-from-assisted-reproductive-technologies-can-be-decreased-by-reproductive-fluids
#14
Sebastian Canovas, Elena Ivanova, Raquel Romar, Soledad García-Martínez, Cristina Soriano-Úbeda, Francisco Alberto A García-Vázquez, Heba Saadeh, Simon Andrews, Gavin Kelsey, Pilar Coy
The number of children born since the origin of Assisted Reproductive Technologies (ART) exceeds 5 million. The majority seem healthy, but a higher frequency of defects has been reported among ART-conceived infants, suggesting an epigenetic cost. We report the first whole-genome DNA methylation datasets from single pig blastocysts showing differences between in vivo and in vitro produced embryos. Blastocysts were produced in vitro either without (C-IVF) or in the presence of natural reproductive fluids (Natur-IVF)...
January 30, 2017: ELife
https://www.readbyqxmd.com/read/28130435/cumulative-live-birth-rates-after-one-art-cycle-including-all-subsequent-frozen-thaw-cycles-in-1050-women-secondary-outcome-of-an-rct-comparing-gnrh-antagonist-and-gnrh-agonist-protocols
#15
M Toftager, J Bogstad, K Løssl, L Prætorius, A Zedeler, T Bryndorf, L Nilas, A Pinborg
STUDY QUESTION: Are cumulative live birth rates (CLBRs) similar in GnRH-antagonist and GnRH-agonist protocols for the first ART cycle including all subsequent frozen-thaw cycles from the same oocyte retrieval? SUMMARY ANSWER: The chances of at least one live birth following utilization of all fresh and frozen embryos after the first ART cycle are similar in GnRH-antagonist and GnRH-agonist protocols. WHAT IS KNOWN ALREADY: Reproductive outcomes of ART treatment are traditionally reported as pregnancies per cycle or per embryo transfer...
January 26, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28127436/sperm-quality-and-paternal-age-effect-on-blastocyst-formation-and-pregnancy-rates
#16
Aurélie Chapuis, Anna Gala, Alice Ferrières-Hoa, Tiffany Mullet, Sophie Bringer-Deutsch, Emmanuelle Vintejoux, Antoine Torre, Samir Hamamah
BACKGROUND: Several studies suggest a decrease in sperm quality in men in the last decades. Therefore, the aim of this work was to assess the influence of male factors (sperm quality and paternal age) on the outcomes of conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). METHODS: This retrospective study included all couples who underwent IVF or ICSI at Montpellier University Hospital, France, between 1 January 2010 and 31 December 2015...
2017: Basic and Clinical Andrology
https://www.readbyqxmd.com/read/28126267/evaluation-of-porcine-stem-cell-competence-for-somatic-cell-nuclear-transfer-and-production-of-cloned-animals
#17
Jan O Secher, Ying Liu, Stoyan Petkov, Yonglun Luo, Dong Li, Vanessa J Hall, Mette Schmidt, Henrik Callesen, Jacob F Bentzon, Charlotte B Sørensen, Kristine K Freude, Poul Hyttel
Porcine somatic cell nuclear transfer (SCNT) has been used extensively to create genetically modified pigs, but the efficiency of the methodology is still low. It has been hypothesized that pluripotent or multipotent stem cells might result in increased SCNT efficacy as these cells are closer than somatic cells to the epigenetic state found in the blastomeres and therefore need less reprogramming. Our group has worked with porcine SCNT during the last 20 years and here we describe our experience with SCNT of 3 different stem cell lines...
January 19, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28123056/human-embryos-cultured-in-vitro-to-14-days
#18
REVIEW
Samantha A Morris
We know a great deal about the development of the mammalian embryo until the time that the blastocyst implants into the uterus. With model organisms such as the mouse, we have also developed a considerable understanding of development immediately around gastrulation as embryos can be recovered at this stage for short-term in vitro culture. However, the intervening period of development remained a 'black box' because it takes place as the blastocyst is implanting into the uterus. Over the past 6 years, techniques pioneered and developed in Magdalena Zernicka-Goetz's laboratory for the in vitro culture of embryos through these implantation stages have opened up this box, affording the first glimpse of embryonic development through these previously hidden stages...
January 2017: Open Biology
https://www.readbyqxmd.com/read/28122009/pre-and-peri-post-compaction-follistatin-treatment-increases-in-vitro-production-of-cattle-embryos
#19
Guo Zhenhua, Sandeep K Rajput, Joseph K Folger, Liu Di, Jason G Knott, George W Smith
Our previous studies demonstrated that maternal (oocyte derived) follistatin (FST) expression is positively associated with bovine oocyte competence and exogenous follistatin treatment during the pre-compaction period of development (d 1-3 post insemination) is stimulatory to bovine early embryogenesis in vitro [blastocyst rates and cell numbers/allocation to trophectoderm (TE)]. In the present study, bovine embryos were treated with exogenous follistatin during d 1-3, d 4-7 and d 1-7 post insemination to test the hypothesis that embryotropic effects of exogenous follistatin are specific to the pre-compaction period (d 1-3) of early embryogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28120355/transporting-bovine-oocytes-in-a-medium-supplemented-with-different-macromolecules-and-antioxidants-effects-on-nuclear-and-cytoplasmic-maturation-and-embryonic-development-in-vitro
#20
M Ambrogi, P C Dall'Acqua, Nas Rocha-Frigoni, Bcs Leão, G Z Mingoti
We investigated whether supplementing the medium used to transport bovine oocytes with different macromolecules [foetal calf serum (FCS) or bovine serum albumin (BSA)] or a mixture of antioxidants (cysteine, cysteamine and catalase) affects their nuclear and cytoplasmic maturation and thereby affects their subsequent embryonic development and cryotolerance. Oocytes were transported for 6 hr in a portable incubator and then subjected to standard in vitro maturation (IVM) for 18 hr. The oocytes in the control groups were cultured (standard IVM) for 24 hr in medium containing 10% FCS (Control FCS) or 10% FCS and the antioxidant mixture (Control FCS+Antiox)...
January 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
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