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Blastocyst transfer

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https://www.readbyqxmd.com/read/28100115/paternal-age-is-not-associated-with-pregnancy-outcomes-after-single-thawed-euploid-blastocyst-transfer
#1
Ashley W Tiegs, Nidhee M Sachdev, Jamie A Grifo, David H McCulloh, Frederick Licciardi
Although controversial, increasing paternal age has been shown to negatively affect assisted reproductive technology (ART) outcomes and success rates. Most studies investigating the effect of paternal age on ART outcomes use a donor oocyte model to minimize maternal aneuploidy contribution. This study sought to determine whether increasing paternal age is associated with adverse in vitro fertilization (IVF) outcomes when aneuploidy is minimized using preimplantation genetic screening. There were 573 single thawed euploid embryo transfers from 473 patients undergoing oocyte donor and autologous IVF cycles...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28093041/blastocyst-development-in-a-single-medium-compared-to-sequential-media
#2
Ioannis A Sfontouris, Efstratios M Kolibianakis, George T Lainas, George K Petsas, Basil C Tarlatzis, Trifon G Lainas
The aim of the present study was to compare blastocyst formation rates after embryo culture in a single medium (Global) as compared to sequential media (ISM1/BlastAssist). In this prospective trial with sibling oocytes, 542 metaphase II (MII) oocytes from 31 women were randomly and equally divided to be fertilized and cultured to the blastocyst stage in either sequential media (ISM1/BlastAssist; n = 271 MII oocytes) or a single medium (Global; n = 271 MII oocytes). In both groups, embryos were cultured in an interrupted fashion with media changes on day 3...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28073983/a-novel-embryo-culture-media-supplement-that-improves-pregnancy-rates-in-mice
#3
A R Highet, T Bianco-Miotto, K G Pringle, A Peura, S Bent, J Zhang, M B Nottle, J G Thompson, C T Roberts
The preimplantation embryo in vivo is exposed to numerous growth factors in the female reproductive tract, which are not recapitulated in embryo culture media in vitro The IGF2 and plasminogen activator systems facilitate blastocyst development. We hypothesized that the addition of IGF2 in combination with urokinase plasminogen activator (uPA) and plasminogen could improve rates of blastocyst hatching and implantation in mice. B6BcF1 and CBAB6F2 mouse embryos were divided into one of four supplemented culture media treatment groups: (1) control (media only); (2) 12...
March 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28070808/combined-positive-effect-of-oocyte-extracts-and-brilliant-cresyl-blue-stained-recipient-cytoplasts-on-epigenetic-reprogramming-and-gene-expression-in-buffalo-nuclear-transfer-embryos
#4
E M Sadeesh, Shah Fozia, Kataria Meena
This study examined the effects of buffalo oocyte extracts (BOE) on donor cells reprogramming and molecular characterisation of oocytes screened via brilliant cresyl blue (BCB) staining and comparison of gene expression profiles of developmentally important genes in blastocysts from IVF and cloned derived from BOE treated donor cells with BCB selected recipient cytoplasts. Relative abundance (RA) of OCT4 and NANOG was increased (P < 0.05) and HDAC-1, DNMT-1, and DNMT-3A decreased (P < 0.05) in extract treated cells (ETCs)...
January 9, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28070710/the-accumulation-of-vitrified-oocytes-is-a-strategy-to-increase-the-number-of-euploid-available-blastocysts-for-transfer-after-preimplantation-genetic-testing
#5
Sandrine Chamayou, Maria Sicali, Carmelita Alecci, Carmen Ragolia, Annalisa Liprino, Daniela Nibali, Giorgia Storaci, Antonietta Cardea, Antonino Guglielmino
PURPOSE: In a preimplantation genetic diagnosis for aneuploidy (PGD-A) program, the more embryos available for biopsy, consequently increases the chances of obtaining euploid embryos to transfer. The aim was to increase the number of viable euploid blastocysts in patients undergoing PGD-A using fresh oocytes together with previously accumulated vitrified oocytes. METHODS: Sixty-nine patients with normal ovarian reserve underwent PGD-A for repeated implantation failure or recurrent pregnancy loss indication...
January 9, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28069172/morphologic-grading-of-euploid-blastocysts-influences-implantation-and-ongoing-pregnancy-rates
#6
Mohamad Irani, David Reichman, Alex Robles, Alexis Melnick, Owen Davis, Nikica Zaninovic, Kangpu Xu, Zev Rosenwaks
OBJECTIVE: To determine whether blastocyst grading can predict pregnancy outcomes in the frozen-thawed embryo transfer (FET) of euploid blastocysts. DESIGN: Retrospective cohort study. SETTING: Academic medical center. PATIENT(S): Women who underwent FET of euploid embryo(s) between January 2013 and December 2015, with blastocysts were divided into four groups based on their morphologic grading before cryopreservation: excellent (≥3AA), good (3-6AB, 3-6BA, 1-2AA), average (3-6BB, 3-6AC, 3-6CA, 1-2AB, 1-2BA), and poor (1-6BC, 1-6CB, 1-6CC, 1-2BB)...
January 6, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28065417/assisted-reproduction-technique-outcomes-for-fresh-versus-deferred-cryopreserved-day-2-embryo-transfer-a-retrospective-matched-cohort-study
#7
Mathilde Bourdon, Pietro Santulli, Vanessa Gayet, Chloé Maignien, Louis Marcellin, Khaled Pocate-Cheriet, Charles Chapron
Ovarian stimulation could adversely affect endometrial receptivity and consequently embryo implantation. One emerging strategy is the 'freeze-all' approach. Most studies have focused on blastocyst transfers, with limited research on day-2 deferred cryopreserved embryo transfers. In this large retrospective cohort study, outcomes were compared between day-2 fresh versus deferred cryopreserved embryo transfers. After matching by age and number of previous cycles, 325 cycles were included in the fresh group and 325 in the deferred cryopreserved embryo transfers group: no significant differences were found between groups in implantation (0...
December 19, 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28062551/new-stimulation-regimens-endogenous-and-exogenous-progesterone-use-to-block-the-lh-surge-during-ovarian-stimulation-for-ivf
#8
REVIEW
Nathalie Massin
BACKGROUND: The advent of embryo and oocyte vitrification today gives reproductive specialists an opportunity to consider new strategies for improving the practice and results of IVF attempts. As the freezing of entire cohorts does not compromise, and may even improve, the results of IVF attempts, it is possible to break away from the standard sequence of stimulation-retrieval-transfer. The constraints associated with ovarian stimulation in relation to the potential harmful effects of the hormonal environment on endometrial receptivity can be avoided...
January 5, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/28055234/scriptaid-upregulates-expression-of-development-related-genes-inhibits-apoptosis-and-improves-the-development-of-somatic-cell-nuclear-transfer-mini-pig-embryos
#9
Li Zhang, Yuemeng Huang, Yanjun Wu, Jinglei Si, Yanna Huang, Qinyang Jiang, Ganqiu Lan, Yafen Guo, Hesheng Jiang
The present study was undertaken to investigate the mechanisms by which Scriptaid treatment improves the developmental competence of somatic cell nuclear transfer (SCNT) mini-pig embryos in vitro. We found that treatment with 500 nmol/L Scriptaid for 15 hours significantly improved the development of mini-pig SCNT embryos. Compared with the control group, the blastocyst rate was higher (18.3% vs. 10.7%; p < 0.05). The acetylation level on H3K14 of the Scriptaid-treated group was higher compared with the control group in SCNT embryos at two-cell, four-cell, and blastocyst stages (p < 0...
January 5, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28052267/the-effect-of-instant-urinary-catheterization-on-pregnancy-outcomes-after-intracytoplasmic-sperm-injection-and-embryo-transfer
#10
Safak Olgan, Enver Kerem Dirican
AIM: To evaluate the effect of instant urinary catheterization on pregnancy rates in women who are on bed rest after embryo transfer (ET). METHODS: This retrospective study included patients who underwent intracytoplasmic sperm injection (ICSI) treatment at the Akdeniz University Fertility Clinic between January 2015 and March 2016. All patients were advised bed rest for an hour after ET. The clinic's electronic database was screened to identify the uterine anatomy of the patients, transferred embryo quality, and urinary catheterization status...
January 5, 2017: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/28050963/live-births-after-polar-body-biopsy-and-frozen-thawed-cleavage-stage-embryo-transfer-case-report
#11
Fernando Guimarães, Matheus Roque, Marcello Valle, Alessandra Kostolias, Rodrigo A de Azevedo, Ciro D Martinhago, Marcos Sampaio, Selmo Geber
Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (blastomere or trophectoderm biopsy). In this case report, we describe the first two live births to be published in Brazil after a polar-body (PB) biopsy. In case 1, a 42-year-old was submitted to PB biopsy with PGS due to advanced maternal age and poor ovarian reserve...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28050958/non-invasive-prediction-of-blastocyst-implantation-ongoing-pregnancy-and-live-birth-by-mass-spectrometry-lipid-fingerprinting
#12
Edson Borges, Daniela P A F Braga, Amanda Souza Setti, Daniela A Montanni, Elaine Cristina Cabral, Marcos N Eberlin, Edson G Lo Turco, Assumpto Iaconelli
OBJECTIVE: To identify lipid markers of blastocyst implantation and ongoing pregnancy by day three culture medium mass spectrometry (MS) fingerprinting. METHODS: For this study, 33 culture media samples were harvested on day three, from 22 patients undergoing day five embryo transfers. All embryos achieved the blastocyst stage and were split into groups based on their implantation (Negative Implantation, n= 14 and Positive Implantation, n= 19). The positive implantation cycles resulted in successful ongoing pregnancies...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28050953/mir-142-3p-as-a-biomarker-of-blastocyst-implantation-failure-a-pilot-study
#13
Edson Borges, Amanda Souza Setti, Daniela P A F Braga, Murilo V Geraldo, Rita de Cássia S Figueira, Assumpto Iaconelli
OBJECTIVE: This study aims to find whether microRNAs (miRNAs) detected in the culture medium of embryos produced in vitro could be potential biomarkers of embryo implantation. METHODS: Culture media samples from 36 embryos, derived from patients undergoing intracytoplasmic sperm injection (ICSI) in a private university-affiliated IVF center, were collected between January/2015 and November/2015. Samples were collected on day three and embryo transfers were performed on day five and all embryos reached the blastocyst stage...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28043656/embryo-and-endometrium-derived-exosomes-and-their-potential-role-in-assisted-reproductive-treatments-liquid-biopsies-for-endometrial-receptivity
#14
REVIEW
Hayden Homer, Gregory E Rice, Carlos Salomon
Multiple pregnancies resulting from the transfer of more than one embryo pose a significant threat to offspring born through Assisted Reproductive Treatments (ART). Transferring one embryo at a time would eliminate this risk. However, current approaches of identifying the highest quality embryo to transfer are either unreliable (e.g. morphology assessment) or highly invasive and potentially detrimental to embryos (e.g. PGD). Approaches for non-invasive embryo selection would be a major advancement that would increase efficiency and reduce both the costs and the risks associated with ART...
December 9, 2016: Placenta
https://www.readbyqxmd.com/read/28042415/chances-to-have-a-boy-after-gender-selection-by-pre-implantation-genetic-screening-are-reduced-in-couples-with-only-girls-and-without-a-boy-sired-by-the-male-partner
#15
Soryya Panahi, Fariba Fahami, Mohammad Reza Deemeh, Marziyeh Tavalaee, Hamid Gourabi, Mohammad Hossain Nasr-Esfahani
BACKGROUND: Gender selection and family planning have their roots in human history. Despite great interest in these fields, very few scientific propositions exist which could explain why some family do not attain the desired sex. Therefore, the aim of this study was to evaluate whether sex of previous child or children could affect the outcomes of pre-implantation genetic screening (PGS). MATERIALS AND METHODS: This historical cohort study including 218 PGS cases referring to Isfahan Fertility and Infertility Center (IFIC)...
January 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28031323/inter-observer-and-intra-observer-agreement-between-embryologists-during-selection-of-a-single-day-5-embryo-for-transfer-a-multicenter-study
#16
Ashleigh Storr, Christos A Venetis, Simon Cooke, Suha Kilani, William Ledger
STUDY QUESTION: What is the inter-observer and intra-observer agreement between embryologists when selecting a single Day 5 embryo for transfer? SUMMARY ANSWER: The inter-observer and intra-observer agreement between embryologists when selecting a single Day 5 embryo for transfer was generally good, although not optimal, even among experienced embryologists. WHAT IS KNOWN ALREADY: Previous research on the morphological assessment of early stage (two pronuclei to Day 3) embryos has shown varying levels of inter-observer and intra-observer agreement...
December 28, 2016: Human Reproduction
https://www.readbyqxmd.com/read/28028774/embryo-wastage-rates-remain-high-in-assisted-reproductive-technology-art-a-look-at-the-trends-from-2004-2013-in-the-usa
#17
Sanaz Ghazal, Pasquale Patrizio
This work examined the trend in "embryo wastage" rates after ART in USA and its relationship to the number of embryos transferred, live born infants delivered across patient age, and the yearly percentage of embryos wasted. The data were obtained from the US-clinics SART databank for the years 2004-2013. A total of 1,808,082 non-donor embryos were transferred in 748,394 fresh cycles resulting in 358,214 liveborn. During the years of analysis, the mean number of embryos transferred has progressively decreased leading to an overall significant decrease in Embryo Wastage rates (83...
December 27, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28017685/physical-exercise-before-pregnancy-helps-the-development-of-mouse-embryos-produced-in-vitro
#18
Wen Hai Xu, Hao Wu, Wei Lan Xia, Hui Lan, Yongsheng Wang, Yong Zhang, Song Hua
Effects of pre-gestational physical activity on the later development of embryos generated in vitro were evaluated. Kunming mice were divided into two groups, namely exercised and unexercised, with the former undergoing physical training on a motor-driven leveled treadmill over a period of 4weeks (5days/week and 60min/day). After that, following superovulation, collection of oocytes from both groups was performed for in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). Notably and specifically, natural mating between the unexercised mice was also done for in vivo fertilization (IVIF)...
December 23, 2016: Mitochondrion
https://www.readbyqxmd.com/read/28004977/establishment-of-transgenic-porcine-fibroblasts-expressing-a-human-klotho-gene-and-its-effects-on-gene-expression-and-preimplantation-development-of-cloned-embryos
#19
Sanghoon Lee, Joon Ho Moon, Kilyoung Song, Anukul Taweechaipaisankul, Young Kwang Jo, Hyun Ju Oh, Se Chang Park, Byeong Chun Lee
Even though the functions of the klotho gene in aging of small animals such as mice have been well investigated, studies using large animal models such as pigs, which have many similarities to humans, have been limited due to the absence of cell lines or animal models. Therefore, the objective of this study was to generate porcine cell lines overexpressing human klotho (hKlotho) and tetracycline (Tet)-inducible hKlotho and to produce cloned embryos from these cell lines. We designed vectors for hKlotho overexpression (CA-Klotho) under control of CMV enhancer/chicken β-actin (CAG) promoter and Tet-inducible hKlotho overexpression (Tet-Klotho, under control of doxycycline-dependent promoter)...
January 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28003868/outcome-of-one-hundred-consecutive-icsi-attempts-using-patient-operated-home-sonography-for-monitoring-follicular-growth
#20
J Gerris, F Vandekerckhove, P De Sutter
OBJECTIVE: cohort study describing clinical and laboratory outcomes in ICSI patients using self-operated endovaginal tele-monitoring (SOET). SETTING: University department of reproductive medicine. PATIENTS: 78 patients undergoing 100 consecutive ICSI attempts. INTERVENTIONS: patients' recorded vaginal sonograms and sent recordings using the cloud based Sonaura device to the care provider who procured responses, avoiding hospital visits...
September 2016: Facts, Views & Vision in ObGyn
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