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Blastocyst transfer

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https://www.readbyqxmd.com/read/28923286/abnormally-fertilized-oocytes-can-result-in-healthy-live-births-improved-genetic-technologies-for-preimplantation-genetic-testing-can-be-used-to-rescue-viable-embryos-in-in-vitro-fertilization-cycles
#1
Antonio Capalbo, Nathan Treff, Danilo Cimadomo, Xin Tao, Susanna Ferrero, Alberto Vaiarelli, Silvia Colamaria, Roberta Maggiulli, Giovanna Orlando, Catello Scarica, Richard Scott, Filippo Maria Ubaldi, Laura Rienzi
OBJECTIVE: To test whether abnormally fertilized oocyte (AFO)-derived blastocysts are diploid and can be rescued for clinical use. DESIGN: Longitudinal-cohort study from January 2015 to September 2016 involving IVF cycles with preimplantation genetic testing for aneuploidy (PGT-A). Ploidy assessment was incorporated whenever a blastocyst from a monopronuclear (1PN) or tripronuclear zygote (2PN + 1 smaller PN; 2.1 PN) was obtained. SETTING: Private IVF clinics and genetics laboratories...
September 15, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28923285/patient-and-cycle-characteristics-predicting-high-pregnancy-rates-with-single-embryo-transfer-an-analysis-of-the-society-for-assisted-reproductive-technology-outcomes-between-2004-and-2013
#2
Jennifer Mersereau, Jamie Stanhiser, Charles Coddington, Tiffany Jones, Barbara Luke, Morton B Brown
OBJECTIVE: To analyze factors associated with high live birth rate and low multiple birth rate in fresh and frozen-thawed assisted reproductive technology (ART) cycles. DESIGN: Retrospective cohort analysis. SETTING: Not applicable. PATIENT(S): The study population included 181,523 women undergoing in vitro fertilization with autologous fresh first cycles, 27,033 with fresh first oocyte donor cycles, 37,658 with fresh second cycles, and 35,446 with frozen-thawed second cycles...
September 15, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28922973/a-new-wnt1-cre-tomatorosa-es-cell-line-a-tool-for-studying-neural-crest-cell-integration-capacity
#3
Soledad Acuna Mendoza, Sabrina Martin, Sabine Kuchler-Bopp, Sandy Ribes, Jeremy H Thalgott, Catherine Chaussain, Sophie Creuzet, Hervé Lesot, Franck Lebrin, Anne Poliard
Neural crest (NC) cells are a migratory, multipotent population giving rise to numerous lineages in the embryo. Their plasticity renders attractive their use in tissue engineering-based therapies but further knowledge on their in vivo behaviour is required before clinical transfer may be envisioned. We here describe isolation and characterization of a new mouse ES line derived from Wnt1-CRE-R26 RosaTomatoTdv blastocyst and show that it displays the characteristics of typical ES cells. Furthermore, these cells can be efficiently directed towards a NC stem cell-like phenotype as attested by concomitant expression of NC marker genes and Tomato fluorescence...
September 19, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28913142/day-3-embryo-transfer-versus-day-5-blastocyst-transfers-a-prospective-randomized-controlled-trial
#4
Şafak Hatırnaz, Mine Kanat Pektaş
OBJECTIVE: This study aimed to show whether transferring day 5 embryos resulted in higher implantation and pregnancy rates than transferring day 3 embryos in Turkish women undergoing an intracytoplasmic sperm injection (ICSI) cycle. MATERIALS AND METHODS: A total of 190 women who had ICSI after retrieval of more than four oocytes on the day of fertilization check were randomly assigned to undergo embryo transfer either on day 3 or day 5. RESULTS: Day 3 and day 5 transfers were statistically similar with respect to the age of woman (p=0...
June 2017: Turk J Obstet Gynecol
https://www.readbyqxmd.com/read/28911212/apposition-to-endometrial-epithelial-cells-activates-mouse-blastocysts-for-implantation
#5
Peter T Ruane, Stéphane C Berneau, Rebekka Koeck, Jessica Watts, Susan J Kimber, Daniel R Brison, Melissa Westwood, John D Aplin
STUDY QUESTION: How do interactions between blastocyst-stage embryos and endometrial epithelial cells regulate the early stages of implantation in an in vitro model? SUMMARY ANSWER: Mouse blastocyst apposition with human endometrial epithelial cells initiates trophectoderm differentiation to trophoblast, which goes on to breach the endometrial epithelium. WHAT IS KNOWN ALREADY: In vitro models using mouse blastocysts and human endometrial cell lines have proven invaluable in the molecular characterisation of embryo attachment to endometrial epithelium at the onset of implantation...
September 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28894983/earlier-day-of-blastocyst-development-is-predictive-of-embryonic-euploidy-across-all-ages-essential-data-for-physician-decision-making-and-counseling-patients
#6
Amy Kaing, Lindsay L Kroener, Robyn Tassin, Man Li, Lian Liu, Richard Buyalos, Gary Hubert, Mousa Shamonki
PURPOSE: The purpose of this study is to evaluate whether day of blastocyst development is associated with embryo chromosomal status as determined by high-density oligonucleotide microarray comparative genomic hybridization (aCGH). METHODS: This is a retrospective cohort analysis, including women who underwent in vitro fertilization (IVF) with trophectoderm biopsy at a single private fertility center from January 2014 to December 2014. Repeat cycles were excluded...
September 11, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28890427/blastocyst-transfer-does-not-improve-cycle-outcome-as-compared-to-d3-transfer-in-antagonist-cycles-with-an-elevated-progesterone-level-on-the-day-of-hcg
#7
Cem Demirel, Serkan Aydoğdu, Arzu İlknur Özdemir, Gülşah Keskin, Ercan Baştu, Faruk Buyru
OBJECTIVE: To evaluate the association between progesterone elevation on the day of human chorionic gonadotropin (hCG) administration and clinical pregnancy rates of gonadotropin-releasing hormone (GnRH) antagonist in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles with the transfer of embryos at different developmental stages (day-3 versus day-5 ETs). MATERIAL AND METHODS: This is a retrospective analysis of fresh IVF/ICSI; 194 cycles out of 2676 conducted in a single center...
September 1, 2017: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/28887104/should-we-consider-day-2-and-day-3-embryo-morphology-before-day-5-transfer-when-blastocysts-reach-a-similar-good-quality
#8
Charlene Herbemont, Solmaz Sarandi, Jeremy Boujenah, Isabelle Cedrin-Durnerin, Nathalie Sermondade, Alexandre Vivot, Christophe Poncelet, Michael Grynberg, Christophe Sifer
Clinical outcomes of 291 day-5 blastocyst transfers carried out between January 2012 and March 2016 were retrospectively compared according to their quality at day 2 and 3. Inclusion criteria were female age younger than 37 years; first or second IVF and intracytoplasmic sperm injection cycle; quality of the transferred blastocyst: blastocoele B3 or higher; inner-cell-mass A/B; trophectoderm A/B; and known implantation outcome for each transferred blastocyst. Blastocysts were classified into good-quality and poor-quality embryo groups at day 2 and 3...
August 4, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28874873/the-overlaying-oil-type-influences-in-vitro-embryo-production-differences-in-composition-and-compound-transfer-into-incubation-medium-between-oils
#9
Cristina A Martinez, Alicia Nohalez, Inmaculada Parrilla, Miguel Motas, Jordi Roca, Inmaculada Romero, Diego L García-Gonza Lez, Cristina Cuello, Heriberto Rodriguez-Martinez, Emilio A Martinez, Maria A Gil
The oil overlay micro-drop system is widely used for cultures of mammalian gametes and embryos. We evaluated hereby the effects of two unaltered commercial oils- Sigma mineral oil (S-MO) and Nidoil paraffin oil (N-PO)-on in vitro embryo production (IVP) outcomes using a pig model. The results showed that while either oil apparently did not affect oocyte maturation and fertilization rates, S-MO negatively affected embryo cleavage rates, blastocyst formation rates, and, consequently, total blastocyst efficiency of the system...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28863936/analysis-of-the-morphological-dynamics-of-blastocysts-after-vitrification-warming-defining-new-predictive-variables-of-implantation
#10
Aila Coello, Marcos Meseguer, Arancha Galán, Lucia Alegre, José Remohí, Ana Cobo
OBJECTIVE: To describe the morphological dynamics of vitrified/warmed blastocysts and to identify quantitative morphological variables related to implantation. Subsequently, by using the most predictive parameters, to develop a hierarchical model by subdividing vitrified/warmed blastocysts into categories with different implantation potentials. DESIGN: Observational, retrospective, cohort study. SETTING: University-affiliated private IVF center...
August 29, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28859279/the-superovulated-environment-independent-of-embryo-vitrification-results-in-low-birthweight-in-a-mouse-model
#11
Rachel Weinerman, Teri Ord, Marisa S Bartolomei, Christos Coutifaris, Monica Mainigi
Epidemiological studies suggest that babies born following in vitro fertilization (IVF) and fresh embryo transfer are of lower birthweight than babies born following frozen embryo transfer, although the mechanism responsible for this phenotype is not known. We developed a novel mouse model that isolates the independent effects of embryo freezing and the superovulated environment, which cannot be performed in humans. We transferred blastocysts that had been vitrified and warmed, mixed with with fresh blastocysts, into individual pseudopregnant recipients produced by either natural mating or mating following injection with equine chorionic gonadotropin and human chorionic gonadotropin and hCG (superovulation)...
July 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28856787/survival-and-developmental-competence-of-bovine-embryos-at-different-developmental-stages-and-separated-blastomeres-after-vitrification-in-different-solutions
#12
Theesit Juanpanich, Tayita Suttirojpattana, Mari Takayama, Yuanyuan Liang, Osamu Dochi, Rangsun Parnpai, Kei Imai
Generating techniques to enhance the success of blastomere separation is important for bovine economy, because it increases the number of transferable embryos. This study aimed to identify the optimum cryoprotectants for the vitrification of bovine embryos and the separation of blastomeres at different stages. In experiment 1, expanded blastocysts were vitrified in two different vitrification solutions, either (1) ethylene glycol (EG) + propylene glycol (PG) or (2) EG. The survival rate of blastocysts in the EG + PG was higher than that of the EG...
August 30, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28851825/embryo-biopsy-and-development-the-known-and-unknown
#13
Federica Zacchini, Roberta Arena, Adam Abramik, Grazyna Ewa Ptak
Preimplantation genetic diagnosis (PGD) has been introduced in clinical practice as a tool for selecting "healthy" embryos before their transfer in utero. PGD protocols include biopsy of cleaving embryos (Blastomere Biopsy - BB) or blastocysts (trophectoderm biopsy - TB), followed by genetic analysis to select "healthy" embryos for transfer in utero. Currently, TB is replacing the use of BB in the clinical practice. However, based on the European Society of Human Reproduction and Embryology Preimplantation Genetic Diagnosis Consortium reports, BB has been used in >87% of PGD cycles for more than 10 years...
August 29, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28839144/the-sex-ratio-of-singleton-and-twin-delivery-offspring-in-assisted-reproductive-technology-in-china
#14
Mengxi Chen, Jiangbo Du, Jing Zhao, Hong Lv, Yifeng Wang, XiaoJiao Chen, Junqiang Zhang, Lingmin Hu, Guangfu Jin, Hongbing Shen, Zhibin Hu, Fang Xiong, Li Chen, Xiufeng Ling
In order to evaluate the impact of assisted reproductive technology (ART) procedure and individual factors on the sex ratio of singletons and twins at birth after in vitro fertilization (IVF) and intra-cytoplasmic sperm injection (ICSI) treatment in China. We conducted a retrospective cohort study including patients undergoing their first cycle of IVF or ICSI with autologous oocytes from 2001 to 2015. A total of 7410 babies were born from 5832 women with 7410 live birth. The secondary sex ratio (SSR) in singletons was significantly higher than twins (53...
August 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28837034/single-versus-sequential-culture-medium-which-is-better-at-improving-ongoing-pregnancy-rates-a-systematic-review-and-meta-analysis
#15
REVIEW
Felipe Dieamant, Claudia G Petersen, Ana L Mauri, Vanessa Comar, Marina Mattila, Laura D Vagnini, Adriana Renzi, Bruna Petersen, Juliana Ricci, João Batista A Oliveira, Ricardo L R Baruffi, Jose G Franco
This study aimed to evaluate if single medium is better than sequential medium at improving ongoing pregnancy rates in patients undergoing assisted reproductive technology (ART) procedures. The data featured in this meta-analysis were extracted from four randomized controlled trials yielded from a systematic search carried out on electronic databases. The primary endpoint was ongoing pregnancy rate. Secondary endpoints included clinical pregnancy and miscarriage rates. The endpoints for ongoing pregnancy rate were also analyzed based on the time at which the embryo transfers were performed: cleavage stage (day 2/3) and/or blastocyst stage (day 5/6)...
September 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28837031/can-ovarian-double-stimulation-in-the-same-menstrual-cycle-improve-ivf-outcomes
#16
Maria Cecília de Almeida Cardoso, Alessandra Evangelista, Cássio Sartório, George Vaz, Caio Luis Vieira Werneck, Fernando Marques Guimarães, Paulo Gallo de Sá, Maria Cecília Erthal
OBJECTIVE: To evaluate the double-stimulation protocol efficacy over conventional ovarian stimulation in recovering a more adequate number of oocytes and increase the number of embryos to be transferred or to be genetically analyzed. METHODS: A retrospective and comparative study with 13 patients who underwent unsuccessful in vitro fertilization (IVF) cycles with a conventional antagonist ovarian stimulation protocol and repeat the attempt with a double stimulation protocol...
September 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28833413/an-improved-vitrification-protocol-for-equine-immature-oocytes-resulting-in-a-first-live-foal
#17
N Ortiz-Escribano, O Bogado Pascottini, H Woelders, L Vandenberghe, C De Schauwer, J Govaere, E Van den Abbeel, T Vullers, C Ververs, K Roels, M Van De Velde, A Van Soom, K Smits
BACKGROUND: The success rate for vitrification of immature equine oocytes is low. Although vitrified-warmed oocytes are able to mature, further embryonic development appears to be compromised. OBJECTIVES: The aim of this study was to compare two vitrification protocols, and to examine the effect of the number of layers of cumulus cells surrounding the oocyte during vitrification of immature equine oocytes. STUDY DESIGN: Experimental in vitro and in vivo trials...
August 20, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28826777/cumulative-live-birth-rate-following-elective-single-blastocyst-transfer-compared-with-double-blastocyst-transfer-in-women-aged-40-years-and-over
#18
Samer Tannus, Yoni Cohen, Weon-Young Son, Tal Shavit, Michael-Haim Dahan
This retrospective cohort study aimed to identify predictive factors for live birth following blastocyst transfer in women aged 40-43, and to compare the cumulative live birth rate (LBR) following elective single blastocyst (eSBT) and double blastocyst (DBT) transfer. The study included 411 women who had fresh blastocyst transfers on day 5. In stepwise logistic regression, independent predictive factors for live birth were: transferring fully expanded blastocysts (Gardner stage ≥3) (odds ratio [OR] 3.5, 95% confidence interval [CI] 1...
August 10, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28826601/significant-correlation-between-anti-m%C3%A3-llerian-hormone-and-embryo-euploidy-in-a-subpopulation-of-infertile-patients
#19
Itai Gat, Basheer AlKudmani, Karen Wong, Khaled Zohni, Noga Fuchs Weizman, Clifford Librach, Prati Sharma
Anti-Müllerian hormone (AMH) is a standard marker of ovarian reserve. Correlation between AMH and egg euploidy is controversial. We evaluated the association between AMH and blastocyst euploidy rate examined by pre-implantation genetic screening (PGS). This retrospective study was conducted at the CReATe Fertility Centre. We included single IVF cycles of 216 infertile couples, which resulted in 911 blastocysts subjected to array comparative genomic hybridization and evaluated IVF outcome after embryo transfer...
August 1, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28822904/the-effect-of-age-and-length-of-gonadotropin-stimulation-on-the-in%C3%A2-vitro-embryo-development-of-holstein-calf-oocytes
#20
Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Werner Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Paulo R da Rosa, Matheus P De Cesaro, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Hernan Baldassarre, Vilceu Bordignon
The use of oocytes recovered from prepubertal donors for in vitro embryo production has great potential for accelerating the rate of genetic gain in the dairy industry. However, these oocytes are known to be less developmentally competent than those from adult donors. In this study, we investigated the effect of age and gonadotropin stimulation in Holstein heifers subjected to oocyte collection every two weeks between 2 and 6 months of age. In order to assess the effect of gonadotropin stimulation, animals were subjected to one of three treatments, namely Short (ST; 36-42 h), Long (LT; ≥72 h) and No Treatment (NT) prior to laparoscopic ovum pick-up (LOPU)...
August 10, 2017: Theriogenology
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