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Blastocyst transfer

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https://www.readbyqxmd.com/read/29778922/interval-of-gonadotropin-administration-for-in-vitro-embryo-production-from-oocytes-collected-from-holstein-calves-between-2-and-6-months-of-age-by-repeated-laparoscopy
#1
Hernan Baldassarre, Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Rafael G Mondadori, Werner G Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Vilceu Bordignon
Laparoscopic Ovum Pick-Up (LOPU) in calves followed by in vitro embryo production (IVEP) and transfer (ET) into adult recipients has great potential for accelerated genetic gain through shortening of the generation interval. In this study, 11 Holstein calves were subjected to up to six LOPU procedures between the ages of 2-6 months at 2-3 weeks interval. In all cases, the animals received a CIDR 5 days prior to LOPU and were gonadotropin-stimulated starting at 72 h before LOPU using one of three protocols that were rotated twice among the animals during the study...
May 17, 2018: Theriogenology
https://www.readbyqxmd.com/read/29766333/treatment-of-donor-cells-with-recombinant-kdm4d-protein-improves-preimplantation-development-of-cloned-ovine-embryos
#2
Yumei Zhang, Qianqian Wang, Kailing Liu, Enen Gao, Hong Guan, Jian Hou
Incomplete epigenetic reprogramming is one of the major factors affecting the development of embryos cloned by somatic cell nuclear transfer (SCNT). Histone 3 lysine 9 (H3K9) trimethylation has been identified as a key barrier to efficient reprogramming by SCNT. The aim of this study was to explore a method of downregulating H3K9me3 levels in donor cells by using histone lysine demethylase (KDM) protein. When sheep fetal fibroblast cells were treated with recombinant human KDM4D protein (rhKDM4D), the levels of H3K9 trimethylation and dimethylation were both significantly decreased...
May 15, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29764212/the-clinical-application-of-single-sperm-based-snp-haplotyping-for-pgd-of-osteogenesis-imperfecta
#3
Linjun Chen, Zhenyu Diao, Zhipeng Xu, Jianjun Zhou, Guijun Yan, Haixiang Sun
Osteogenesis imperfecta (OI) is a genetically heterogeneous disorder, presenting either autosomal dominant, autosomal recessive or X-linked inheritance patterns. The majority of OI cases are autosomal dominant and are caused by heterozygous mutations in either the COL1A1 or COL1A2 gene. In these dominant disorders, allele dropout (ADO) can lead to misdiagnosis in preimplantation genetic diagnosis (PGD). Polymorphic markers linked to the mutated genes have been used to establish haplotypes for identifying ADO and ensuring the accuracy of PGD...
May 15, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29758560/prospective-randomized-trial-comparing-embryo-transfers-of-cases-with-and-without-catheter-rotation-during-its-withdrawal
#4
Cigdem Yayla Abide, Enis Ozkaya, Ilhan Sanverdi, Evrim Bostancı Ergen, Meryem Kurek Eken, Belgin Devranoglu, Bulent Emre Bilgiç, Cetin Kilicci, Semra Kayatas Eser
OBJECTIVE: To compare embryo transfer (ET) technique based on catheter rotation during its withdrawal in cases with unexplained infertility in a prospective, randomized trial (NCT03097042). METHODS: Two hundred intracytoplasmic sperm injection (ICSI) patients undergoing ET with cleaving or blastocyst-stage fresh embryos were randomized into 2 groups: cases with (n = 100), and without (n = 100) catheter rotation during its withdrawal. Groups were matched for age and some clinical parameters...
May 14, 2018: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/29751777/high-efficiency-derivation-of-human-embryonic-stem-cell-lines-using-a-culture-system-with-minimized-trophoblast-cell-proliferation
#5
Chuti Laowtammathron, Pimjai Chingsuwanrote, Roungsin Choavaratana, Suphadtra Phornwilardsiri, Ketsara Sitthirit, Chidchanok Kaewjunun, Orawan Makemaharn, Papussorn Terbto, Supaporn Waeteekul, Chanchao Lorthongpanich, Yaowalak U-Pratya, Pimonwan Srisook, Pakpoom Kheolamai, Surapol Issaragrisil
BACKGROUND: Due to their extensive self-renewal and multilineage differentiation capacity, human embryonic stem cells (hESCs) have great potential for studying developmental biology, disease modeling, and developing cell replacement therapy. The first hESC line was generated in 1998 by culturing inner cell mass (ICM) cells isolated from human blastocysts using an immunosurgery technique. Since then, many techniques including mechanical ICM isolation, laser dissection, and whole embryo culture have been used to derive hESC lines...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29740477/determination-of-oocyte-manipulation-zygote-manipulation-and-genome-reprogramming-effects-on-the-transcriptomes-of-bovine-blastocysts
#6
Byungkuk Min, Jung S Park, Yong-Kook Kang
Somatic cell nuclear transfer (scNT) embryos suffer from damage caused by micro-operation (manipulation) and inefficient genome reprograming that hinder their normal development at different levels and in distinct ways. These two effects are inseparable in the nature of the scNT embryo, although methods to separately measure them are needed to improve scNT technology and evaluate incoming reprogramming tools. As an attempt to meet these demands, we made bovine sham nuclear-transfer (shNT) blastocysts, special embryos made with a standard nuclear-transfer procedure at the zygote stage, while retaining an intact genome...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/29740154/additional-mitochondrial-dna-influences-the-interactions-between-the-nuclear-and-mitochondrial-genomes-in-a-bovine-embryo-model-of-nuclear-transfer
#7
Kanokwan Srirattana, Justin C St John
We generated cattle embryos using mitochondrial supplementation and somatic cell nuclear transfer (SCNT), named miNT, to determine how additional mitochondrial DNA (mtDNA) modulates the nuclear genome. To eliminate any confounding effects from somatic cell mtDNA in intraspecies SCNT, donor cell mtDNA was depleted prior to embryo production. Additional oocyte mtDNA did not affect embryo development rates but increased mtDNA copy number in blastocyst stage embryos. Moreover, miNT-derived blastocysts had different gene expression profiles when compared with SCNT-derived blastocysts...
May 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29737471/does-the-endometrial-receptivity-array-really-provide-personalized-embryo-transfer
#8
Rawad Bassil, Robert Casper, Nivin Samara, Tzu-Bou Hsieh, Eran Barzilay, Raoul Orvieto, Jigal Haas
PURPOSE: The aim of the present study was to determine the percentage of infertility patients who are diagnosed with a non-receptive endometrium according to the endometrial receptivity array (ERA) test and to examine whether adjusting the embryo transfer day according to the proposed shift in the window of implantation improves the pregnancy rate compared to non-ERA-tested patients. METHODS: A single-center retrospective cohort study, including 53 consecutive good prognosis patients (0-2 previous frozen embryo transfers) admitted to our IVF unit for a mock cycle prior to their frozen day-5 embryo (blastocyst) transfer cycle...
May 8, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29734409/an-in-vitro-assessment-of-the-value-of-sucrose-supplementation-to-warming-media-for-the-vitrification-of-bovine-embryos
#9
V H Do, S Watson, S Catt, A W Taylor-Robinson
  BACKGROUND: In order to thaw slow-cooled bovine embryos it is standard practice to draw out permeating cryoprotectants by passing embryos through successively decreasing osmotic solutions. However, recently it has been suggested that sucrose may not be needed in the warming media. OBJECTIVE: The aim of this experiment was to compare the effect of warming media prepared with or without the inclusion of sucrose on the survival and hatching capacity of vitrified in vitro-derived bovine embryos...
September 2017: Cryo Letters
https://www.readbyqxmd.com/read/29734407/effect-of-developmental-stage-of-embryos-at-freezing-on-live-birth-outcomes-after-frozen-embryo-transfer
#10
X J Zhang, Q Lv, L H Min, X H Cao, X J Li
  Purpose Our objective was to evaluate the birth outcomes of FET from different developmental stage of embryo at freezing (Day 3, Day 5 and Day 6). Methods All vitrified-warmed day 3 (D3) and day 5/6 (D5, D6) embryo transfers during the time period from January 2013 until December 2015 were included in the study. The Birthweight, Low birthweight (LBW), sexual distinction, gestation and for singleton births and twins conceived by FET were compared retrospectively between three cryopreservation strategies utilizing either D3, D5 or D6 embryo freezing...
September 2017: Cryo Letters
https://www.readbyqxmd.com/read/29734084/association-between-early-%C3%AE-hcg-kinetics-blastocyst-morphology-and-pregnancy-outcome-in-a-single-blastocyst-transfer-program
#11
Jean-Maxime Girard, Mathilde Simorre, Florence Leperlier, Arnaud Reignier, Tiphaine Lefebvre, Paul Barrière, Thomas Fréour
OBJECTIVE(S): Beta human chorionic gonadotrophin (β-hCG) is secreted by placenta and detectable in maternal serum a few days after embryo implantation. The evolution of β-hCG serum levels is commonly used to confirm early pregnancy and differentiate normal pregnancies from others, either in spontaneous pregnancies or after IVF. However, little is known on the eventual link between blastocyst quality in IVF, pregnancy outcome, and βhCG kinetics. The objective was to evaluate the association between early βhCG rise, blastocyst morphology and pregnancy evolution in a single-blastocyst transfer program STUDY DESIGN: This retrospective cohort study was conducted in a University-affiliated IVF center in 441 couples undergoing 455 single blastocyst transfer cycles leading to a positive pregnancy test 12 days afterwards...
April 30, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29733525/m-carboxycinnamic-acid-bishydroxamide-improves-developmental-competence-reduces-apoptosis-and-alters-epigenetic-status-and-gene-expression-pattern-in-cloned-buffalo-bubalus-bubalis-embryos
#12
H Agrawal, N L Selokar, M Saini, M K Singh, M S Chauhan, P Palta, S K Singla, R S Manik
Incomplete or aberrant reprogramming of nuclear genome is one of the major problems in somatic cell nuclear transfer. In this study, we studied the effect of histone deacetylase inhibitor m-carboxycinnamic acid bishydroxamide (CBHA) on in vitro development of buffalo embryos produced by Hand-made cloning. Cloned embryos were treated with CBHA (0, 5, 10, 20 or 50 μM) for 10 hr from the start of reconstruction till activation. At 10 μM, but not at other concentrations examined, CBHA increased (p < ...
May 7, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29728797/potential-of-testicular-sperm-to-support-embryonic-development-to-the-blastocyst-stage-is-comparable-to-that-of-ejaculated-sperm
#13
Sun-Hee Lee, Chan Woo Park, Yong-Pil Cheon, Chun Kyu Lim
PURPOSE: Recent studies have shown that improved clinical outcomes can be achieved by transferring blastocysts rather than cleavage-stage embryos. However, blastocyst transfer is not performed in all patients. The aim of this study was to compare clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles using testicular sperm (TE) with those of ICSI cycles using ejaculated sperm (EJ). METHODS: ICSI was performed using EJ in 141 cycles and TE in 37 cycles...
May 5, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29725911/continuous-embryo-culture-elicits-higher-blastulation-but-similar-cumulative-delivery-rates-than-sequential-a-large-prospective-study
#14
Danilo Cimadomo, C Scarica, R Maggiulli, G Orlando, D Soscia, L Albricci, S Romano, F Sanges, F M Ubaldi, L Rienzi
PURPOSE: To assess whether continuous embryo culture involves better embryological and/or clinical outcomes than sequential. METHODS: Prospective study at a private IVF center. All consecutive IVF cycles (September 2013-2015) fulfilling the inclusion criteria underwent embryo culture in either Continuous-Single-Culture-Media (CSCM, n = 972) or sequential media (Quinn's Advantage, n = 514), respectively. ICSI, blastocyst culture in either standard (MINC) or undisturbed (Embryoscope) incubation, transfer (until September 2016), and pregnancy follow-up (until September 2017) were performed...
May 3, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29725050/efficient-generation-of-transgenic-buffalos-bubalus-bubalis-by-nuclear-transfer-of-fetal-fibroblasts-expressing-enhanced-green-fluorescent-protein
#15
Fenghua Lu, Chan Luo, Nan Li, Qingyou Liu, Yingming Wei, Haiying Deng, Xiaoli Wang, Xiangping Li, Jianrong Jiang, Yanfei Deng, Deshun Shi
The possibility of producing transgenic cloned buffalos by nuclear transfer of fetal fibroblasts expressing enhanced green fluorescent protein (EGFP) was explored in this study. When buffalo fetal fibroblasts (BFFs) isolated from a male buffalo fetus were transfected with pEGFP-N1 (EGFP is driven by CMV and Neo is driven by SV-40) by means of electroporation, Lipofectamine-LTX and X-tremeGENE, the transfection efficiency of electroporation (35.5%) was higher than Lipofectamine-LTX (11.7%) and X-tremeGENE (25...
May 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29722953/can-trophectoderm-morphology-act-as-a-predictor-for-euploidy
#16
Ivan H Yoshida, Monise Santos, Caroline Z Berton, Caroline L Chiarella, Michelli S Tanada, Emerson BCordts, Waldemar P de Carvalho, Caio P Barbosa
OBJECTIVE: Euploid embryo transfers yield better implantation rates. In Brazil, morphological evaluation is performed to select the best embryos, since genetic analysis is still an expensive procedure. This study aimed to evaluate whether there is an association between trophectoderm morphology and ploidy status. METHODS: The study included 113 blastocysts formed in D5/D6 from 58 in vitro fertilization cycles held from January/2016 to May/2017. All patients with indication for PGD/PGS were included in the study...
May 3, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29720000/a-comparison-of-the-survival-and-implantation-rates-of-blastocysts-that-were-vitrified-on-post-fertilization-day-five-six-and-seven
#17
Avci Berrin, Kasapoglu Isıl, Ata Baris, Kuspinar Goktan, Saribal Seda, Uncu Gurkan
The goal of this retrospective cohort study was to compare survival, implantation, clinical and ongoing pregnancy rates between blastocysts that were vitrified on post-fertilization days 5, 6 and 7. Before vitrification, blastocysts were evaluated in terms of morphology and blastocyst expansion, inner cell mass and trophectoderm quality. They were thawed and transfered in a subsequent artificial cycle. Embryo implantation rates were 39%, 25% and 25% for blastocysts that were vitrified on days 5, 6, and 7, respectively (p = 0...
May 3, 2018: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29718981/tauroursodeoxycholic-acid-tudca-alleviates-endoplasmic-reticulum-stress-of-nuclear-donor-cells-under-serum-starvation
#18
Ying Zhang, Pengxiang Qu, Xiaonan Ma, Fang Qiao, Yefei Ma, Suzhu Qing, Yong Zhang, Yongsheng Wang, Wei Cui
Serum starvation is a routine protocol for synchronizing nuclear donor cells to G0/G1 phase during somatic cell nuclear transfer (SCNT). However, abrupt serum deprivation can cause serious stress to the cells cultured in vitro, which might result in endoplasmic reticulum (ER) stress, chromosome damage, and finally reduce the success rate of SCNT. In the present study, the effects of tauroursodeoxycholic acid (TUDCA), an effective ER stress-relieving drug, on the nuclear donor cells under serum deprivation condition as well as following SCNT procedures were first assessed in the bovine...
2018: PloS One
https://www.readbyqxmd.com/read/29708768/fibroblast-growth-factor-10-enhances-the-developmental-efficiency-of-somatic-cell-nuclear-transfer-embryos-by-accelerating-the-kinetics-of-cleavage-during-in-vitro-maturation
#19
Yeo-Jin Son, Seung-Eun Lee, Yun-Gwi Park, Sang-Gi Jeong, Min-Young Shin, Eun-Young Kim, Se-Pill Park
Somatic cell nuclear transfer (SCNT) is required for the generation of transgenic animals as disease models. During the in vitro development of SCNT embryos, the quality of matured oocytes is one of the major factors regulating the developmental potential of embryos. Time-lapse monitoring systems are new tools that assess the developmental capacity of embryos for use in embryo transfer. In this study, we investigated the effect of fibroblast growth factor 10 (FGF 10) on the developmental potential of SCNT embryos...
April 30, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29707686/analysis-of-endoplasmic-reticulum-er-stress-induced-during-somatic-cell-nuclear-transfer-scnt-process-in-porcine-scnt-embryos
#20
Hwa-Yeon Lee, Hyo-Kyung Bae, Bae-Dong Jung, Seunghyung Lee, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
This study investigates the endoplasmic reticulum (ER) stress and subsequent apoptosis in duced during somatic cell nuclear transfer (SCNT) process of porcine SCNT embryos. Porcine SCNT and in vitro fertilization (IVF) embryos were sampled at 3 h and 20 h after SCNT or IVF and at the blastocyst stage for mRNA extraction. The x-box binding protein 1 (Xbp1) mRNA and the expressions of ER stress-associated genes were confirmed by RT-PCR or RT-qPCR. Apoptotic gene expression was analyzed by RT-PCR. Before commencing SCNT, somatic cells treated with tunicamycin (TM), an ER stress inducer, confirmed the splicing of Xbp1 mRNA and increased expressions of ER stress-associated genes...
March 2018: Balsaeng'gwa Saengsig
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