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https://www.readbyqxmd.com/read/27928970/effect-of-transfection-and-co-incubation-of-bovine-sperm-with-exogenous-dna-on-sperm-quality-and-functional-parameters-for-its-use-in-sperm-mediated-gene-transfer
#1
María Elena Arias, Esther Sánchez-Villalba, Andrea Delgado, Ricardo Felmer
Sperm-mediated gene transfer (SMGT) is based on the capacity of sperm to bind exogenous DNA and transfer it into the oocyte during fertilization. In bovines, the progress of this technology has been slow due to the poor reproducibility and efficiency of the production of transgenic embryos. The aim of the present study was to evaluate the effects of different sperm transfection systems on the quality and functional parameters of sperm. Additionally, the ability of sperm to bind and incorporate exogenous DNA was assessed...
December 8, 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27925011/post-harvest-embryo-development-in-ginseng-seeds-increases-desiccation-sensitivity-and-narrows-the-hydration-window-for-cryopreservation
#2
E Han, E Popova, G Cho, S Park, S Lee, H W Pritchard, H H Kim
BACKGROUND: Despite its self-pollinating characteristics, Korean ginseng germplasm is mainly maintained in clonal gene banks as there is no defined approach to the long-term conservation of its seed, including the most appropriate stage of embryo development for storage. OBJECTIVE: The aim of this study was to reveal the effect of embryo development on desiccation tolerance and cryopreservation success in ginseng seeds. MATERIALS AND METHODS: Seeds of Korean ginseng (Panax ginseng C...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27925005/seed-desiccation-tolerance-and-cryopreservation-of-philippine-calamansi-x-citrofortunella-microcarpa-bunge-wijnands
#3
O L Damasco, L C Refuerzo
BACKGROUND: The traditional on farm conservation of Calamansi [x Citrofortunella microcarpa (Bunge) Wijnands], an important indigenous Citrus species in the Philippines, is now being threatened by shifting agricultural crop production, climate change, and increasing biotic and abiotic stresses. OBJECTIVE: The study aimed to characterize the desiccation and cryopreservation tolerance of seeds as the basis for complementary long term ex situ conservation. MATERIALS AND METHODS: Intact seeds were desiccated in an airtight container filled with activated silica gel for 0-96 h...
July 2016: Cryo Letters
https://www.readbyqxmd.com/read/27923412/effects-of-vitrification-on-blastomere-viability-and-cytoskeletal-integrity-in-mouse-embryos
#4
Zenon Oikonomou, Katerina Chatzimeletiou, Antonia Sioga, Louisa Oikonomou, Basil C Tarlatzis, Efstratios Kolibianakis
Vitrification is widely used to cryopreserve supernumerary embryos following in vitro fertilization (IVF). The mouse model was used to investigate the effects of vitrification on blastomere viability, using viability markers, and on the cytoskeleton, by analysing spindle/chromosome configurations, using confocal scanning microscopy. Ninety cleavage and morula stage dimethyl sulphoxide (DMSO)/EG vitrified mouse embryos were either processed immediately following warming for viability assessment by labelling with the fluorescent markers carboxyfluorescein-diacetate succinimidylester (CFSE) and propidium iodide (PI) or were cultured to the blastocyst stage and immunostained with α-tubulin antibody to visualize microtubules and DAPI or PI to visualize DNA...
December 7, 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27920558/monitoring-the-effects-of-doxorubicin-on-3d-spheroid-tumor-cells-in-real-time
#5
NamHuk Baek, Ok Won Seo, MinSung Kim, John Hulme, Seong Soo A An
Recently, increasing numbers of cell culture experiments with 3D spheroids presented better correlating results in vivo than traditional 2D cell culture systems. 3D spheroids could offer a simple and highly reproducible model that would exhibit many characteristics of natural tissue, such as the production of extracellular matrix. In this paper numerous cell lines were screened and selected depending on their ability to form and maintain a spherical shape. The effects of increasing concentrations of doxorubicin (DXR) on the integrity and viability of the selected spheroids were then measured at regular intervals and in real-time...
2016: OncoTargets and Therapy
https://www.readbyqxmd.com/read/27918727/the-current-state-of-reproductive-biology-research-in-australia-and-new-zealand-core-themes-from-the-society-for-reproductive-biology-annual-meeting-2016
#6
L K Akison, P H Andraweera, M J Bertoldo, H M Brown, J S M Cuffe, T Fullston, O Holland, J E Schjenken
Because reproduction is essential for all life, it is central to our understanding of all aspects of biology. The Society for Reproductive Biology (SRB) 2016 conference held on the Gold Coast (Qld, Australia) displayed the current breadth of reproductive research in Australia and New Zealand, with additional insights from world leaders in the field. This conference review provides a focused summary of the key questions, emerging ideas and novel technologies that were presented in the symposia. Presented research demonstrated key advances in how stem cell biology may allow us to better understand pluripotency, as well as how environmental and lifestyle factors, such as circadian disruption, smoking, alcohol and diet, affect gametogenesis, embryo implantation, placental function and reproductive capacity...
December 6, 2016: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/27908686/effect-of-different-vitrification-solutions-and-cryodevices-on-viability-and-subsequent-development-of-buffalo-oocytes-vitrified-at-the-germinal-vesicle-gv-stage
#7
Amr S El-Shalofy, Adel R Moawad, Gamal M Darwish, Sayed T Ismail, Aly B A Badawy, Magdy R Badr
The cryopreservation of immature oocytes would generate a readily available, non-seasonal source of female gametes for research and reproduction. In domestic animals, the most promising results on oocyte cryopreservation have been reported in cattle, few studies have been conducted on buffalo. The aim of the present study was to compare the use of different vitrification solutions and various cryodevices on viability and developmental competence of buffalo oocytes vitrified at the germinal vesicle (GV) stage...
November 28, 2016: Cryobiology
https://www.readbyqxmd.com/read/27901197/in-vitro-germination-of-zygotic-embryos-of-hybrid-brs-manicor%C3%A3-e-guineensis-x-e-oleifera
#8
Keila A P Bonetti, Marguerite Quoirin, Regina C Quisen, Suelen C S Lima
The interspecific oil palm hybrid BRS Manicoré (E. guineensis x E. oleifera) has superior agronomic characteristics. However, the germination rate is low (30%) and the process is slow when the seeds are sown in a conventional form. The purpose of this study was to optimize the in vitro germination of zygotic embryos of this hybrid comparing seed lots. The viability of zygotic embryos was evaluated by the tetrazolium test (0.075%) for 4 h. The embryos were cultured on MS and Y3 culture media, with and without the addition of NaH2PO4, as well as on MS, MS1/2 and N6 medium...
2016: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/27894826/artemin-protects-cells-and-proteins-against-oxidative-and-salt-stress
#9
Zeinab Takalloo, Reza H Sajedi, Saman Hosseinkhani, Taghi Moazzenzade
Artemin is an abundant thermostable protein in Artemia encysted embryos under environmental stresses. It is confirmed that high regulatory expression of artemin is relevant to stress resistance in this crustacean. Here, the protective role of artemin from Artemia urmiana has been investigated on survival of bacterial cells under salt and oxidative shocks. Also, for continuous monitoring of the effect of artemin in prevention of proteins aggregation/inactivation, co-expression of artemin and luciferase (as an intracellular reporter) in bacterial cells was performed...
November 25, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27881437/casein-kinase-ii-is-required-for-proper-cell-division-and-acts-as-a-negative-regulator-of-centrosome-duplication-in-c-elegans-embryos
#10
Jeffrey C Medley, Megan M Kabara, Michael D Stubenvoll, Lauren E DeMeyer, Mi Hye Song
Centrosomes are the primary microtubule-organizing centers that orchestrate microtubule dynamics during the cell cycle. The correct number of centrosomes is pivotal for establishing bipolar mitotic spindles that ensure accurate segregation of chromosomes. Thus, centrioles must duplicate once per cell cycle, one daughter per mother centriole, the process of which requires highly coordinated actions among core factors and modulators. Protein phosphorylation is shown to regulate the stability, localization and activity of centrosome proteins...
November 23, 2016: Biology Open
https://www.readbyqxmd.com/read/27881070/pregnancy-specific-%C3%AE-1-glycoproteins-combined-biomarker-roles-structure-function-relationships-and-implications-for-drug-design
#11
Nurbubu T Moldogazieva, Innokenty M Mokhosoev, Alexander A Terentiev
BACKGROUND: Pregnancy specific β1-glycoproteins (PSGs) have long been recognized as trophoblast quality and embryo viability markers. However, biological roles of PSGs remain obscure, and structure/function relationships to other feto-placental proteins as well as implications for drug design have not been reviewed. This review summarizes and discusses advances in 45-year studies of PSGs with focus on the latest achievements and the challenges for future investigations. METHODS: Literature search was performed to review the majority of recent PSG studies with emphasis on usage of high-throughput integrated proteomic profiling technologies, systems biology and bioinformatics approaches that enhance novel biomarker and drug target discovery as well as protein structure/activity analysis...
November 22, 2016: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/27878294/arctigenin-a-lignan-from-arctium-lappa-l-inhibits-metastasis-of-human-breast-cancer-cells-through-the-downregulation-of-mmp-2-9-and-heparanase-in-mda-mb-231-cells
#12
Chenghua Lou, Zhihui Zhu, Yaping Zhao, Rui Zhu, Huajun Zhao
Arctigenin is a bioactive lignan isolated from the seeds of Arctium lappa L. which has been widely used as a diuretic and a diaphoretic in Traditional Chinese Medicine. In the present study, the authors investigated the effects of arctigenin on tumor migration and invasion in aggressive human breast cancer cells. The MTT assay results showed that arctigenin did not show a significant cytotoxic effect on the cell viability of MDA-MB-231 cells. However, wound healing migration and Boyden chamber invasion assays demonstrated that arctigenin significantly inhibited in vitro migration and invasion of the MDA-MB-231 cells...
November 23, 2016: Oncology Reports
https://www.readbyqxmd.com/read/27865408/progesterone-and-ultrasonographic-changes-during-aglepristone-or-cloprosternol-treatment-in-queens-at-21-to-22-or-35-to-38%C3%A2-days-of-pregnancy
#13
M C García Mitacek, M C Bonaura, R G Praderio, R Nuñez Favre, R L de la Sota, M A Stornelli
Progesterone (P4) is a requirement for pregnancy development. Previous reports observed a maximal value of serum P4 concentration on 21 days after the first mating after which it slowly declines throughout the rest of pregnancy. Ultrasound examination should be performed to ensure that pregnancy interruption is complete. Limited information is available on the ultrasonic appearance of conceptuses during pregnancy termination in cats The objective was to study serum P4 concentration and ultrasonographic changes during aglepristone (ALI) or cloprostenol (CLO) treatment and to evaluate the fertility after treatment...
January 15, 2017: Theriogenology
https://www.readbyqxmd.com/read/27862395/the-nuclear-and-developmental-competence-of-cumulus-oocyte-complexes-is-enhanced-by-three-dimensional-coculture-with-conspecific-denuded-oocytes-during-in-vitro-maturation-in-the-domestic-cat-model
#14
M G Morselli, G C Luvoni, P Comizzoli
The objective of the study was to assess the efficacy of coculture with conspecific cumulus-denuded oocytes (CDOs) during in vitro maturation in a three-dimensional system of barium alginate microcapsules on the in vitro embryo development of domestic cat cumulus-oocyte complexes (COCs). In Experiment I, COCs were cocultured with conspecific CDOs or cultured separately in a 3D system for 24 hr of in vitro maturation, before assessing the meiotic progression. In Experiment II, the in vitro fertilization of COCs and CDOs was carried out with chilled epididymal spermatozoa and the presumptive zygotes were cultured in vitro separately for 7 days in 3D microcapsules before assesment of embryonic development...
November 15, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27859529/porcine-sperm-vitrification-ii-spheres-method
#15
C C Arraztoa, M H Miragaya, M G Chaves, V L Trasorras, M C Gambarotta, D M Neild
Owing to current problems in boar sperm cryopreservation, this study proposes to evaluate vitrification in spheres as an alternative cryopreservation procedure, comparing the use or not of permeable cryoprotectants and two warming methods. Extended (n = 3; r = 4) and raw (n = 5; r = 2) porcine spermatozoa were diluted in media, in the absence or presence of either 4% dimethylformamide or 4% glycerol, to a final concentration of 5 × 10(6)  spermatozoa/ml and vitrified using the spheres method. Two warming procedures were evaluated: a rapid method (30 s at 37°C) and an ultrarapid method (7 s at 75°C, followed by 30 s at 37°C)...
November 10, 2016: Andrologia
https://www.readbyqxmd.com/read/27845743/a-structure-identification-and-toxicity-assessment-of-the-degradation-products-of-aflatoxin-b%C3%A2-in-peanut-oil-under-uv-irradiation
#16
Jin Mao, Bing He, Liangxiao Zhang, Peiwu Li, Qi Zhang, Xiaoxia Ding, Wen Zhang
Aflatoxins, a group of extremely hazardous compounds because of their genotoxicity and carcinogenicity to human and animals, are commonly found in many tropical and subtropical regions. Ultraviolet (UV) irradiation is proven to be an effective method to reduce or detoxify aflatoxins. However, the degradation products of aflatoxins under UV irradiation and their safety or toxicity have not been clear in practical production such as edible oil industry. In this study, the degradation products of aflatoxin B₁ (AFB₁) in peanut oil were analyzed by Ultra Performance Liquid Chromatograph-Thermo Quadrupole Exactive Focus mass spectrometry/mass spectrometry (UPLC-TQEF-MS/MS)...
November 12, 2016: Toxins
https://www.readbyqxmd.com/read/27807889/viability-and-growth-of-feline-preantral-follicles-in-vitro-cultured-with-insulin-growth-factor-and-epidermal-growth-factor-supplemented-medium
#17
A E Alves, L C Padilha-Nakaghi, E A Pires-Butler, M Apparicio, Nam Silva, T F Motheo, Wrr Vicente, G C Luvoni
In vitro culture of ovarian preantral follicles has emerged as a reproductive technology aimed at obtaining large amount of oocytes for in vitro embryo production. The addition of growth factors (GF) in the in vitro culture of preantral follicles of different species has provided superior results of follicular development, antrum formation and proliferation of granulosa cells. However, there are only few reports regarding the use of these factors on feline preantral follicle in vitro culture. Thus, the aim of this study was to investigate the effect of a combination of IGF-1 and EGF on in vitro viability and growth of preantral follicles and enclosed oocytes collected from domestic cats...
November 3, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27807789/cytotoxicity-and-oxidative-stress-responses-of-silica-coated-iron-oxide-nanoparticles-in-chse-214-cells
#18
K Srikanth, Tito Trindade, A C Duarte, E Pereira
The present study aimed at investigating cytotoxicity and oxidative stress induced by silica-coated iron oxide nanoparticles functionalized with dithiocarbamate (Fe3O4 NPs) in Chinook salmon cells (CHSE-214) derived from Oncorhynchus tshawytscha embryos. A significant reduction in cell viability was evident in response to Fe3O4 NPs as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 24 h of exposure. Out of the tested concentrations (10, 20, and 30 μg/ml), the highest concentration has shown significant decrease in the viability of cells after 24 h of exposure...
November 3, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27805544/analyses-of-apoptosis-and-dna-damage-in-bovine-cumulus-cells-after-in-vitro-maturation-with-different-copper-concentrations-consequences-on-early-embryo-development
#19
D E Rosa, J M Anchordoquy, J P Anchordoquy, M A Sirini, J A Testa, G A Mattioli, C C Furnus
The aim of this study was to investigate the influence of copper (Cu) during in vitro maturation (IVM) on apoptosis and DNA integrity of cumulus cells (CC); and oocyte viability. Also, the role of CC in the transport of Cu during IVM was evaluated on oocyte developmental capacity. Damage of DNA was higher in CC matured without Cu (0 µg/dl Cu, P < 0.01) with respect to cells treated with Cu for cumulus-oocyte complexes (COCs) exposed to 0, 20, 40, or 60 µg/dl Cu). The percentage of apoptotic cells was higher in CC matured without Cu than in CC matured with Cu...
December 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27780844/mouse-models-of-casc3-reveal-developmental-functions-distinct-from-other-components-of-the-exon-junction-complex
#20
Hanqian Mao, Hannah E Brown, Debra L Silver
The exon junction complex (EJC) is a multi-protein complex integral to RNA metabolism. Biochemistry and genetic studies have concluded that the EJC is composed of 4 core proteins, MAGOH, EIF4A3, RBM8A, and CASC3. Yet recent studies in Drosophila indicate divergent functions for Barentsz, the mammalian Casc3 ortholog, raising the question as to whether CASC3 is a constitutive component of the EJC. This issue remains poorly understood, particularly in an in vivo mammalian context. We previously found that haploinsufficiency for Magoh, Eif4a3, or Rbm8a disrupts neuronal viability and neural progenitor proliferation, resulting in severe microcephaly...
October 25, 2016: RNA
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