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https://www.readbyqxmd.com/read/29670362/antiangiogenic-effects-of-aa-pme-on-huvecs-in-vitro-and-zebrafish-in-vivo
#1
Yue Jing, Gang Wang, Qi Xiao, Yachun Zhou, Yingjie Wei, Zhunan Gong
Angiogenesis plays a vital role in many physiological and pathological processes and several diseases are connected with its dysregulation. Asiatic acid (AA) has demonstrated anticancer properties and we suspect this might be attributable to an effect on angio-genesis. A modified derivative of AA, N-(2α,3β,23-acetoxyurs-12-en-28-oyl)-L-proline methyl ester (AA-PMe), has improved efficacy over its parent compound, but its effect on blood vessel development remains unclear. Methods: In this study, we investigated the antiangiogenic activity of AA and AA-PMe in zebrafish embryos and human umbilical vein endothelial cells (HUVECs)...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29664793/extracellular-vesicles-a-promising-tool-for-assessment-of-embryonic-competence
#2
Diego Marin, Richard T Scott
PURPOSE OF REVIEW: Extracellular vesicles have recently emerged as a promising field of research due to their pivotal roles in intercellular communication and potential to serve as biomarkers. This review focuses on extracellular vesicles secreted by the human preimplantation embryo. The most recent findings on embryo-derived extracellular vesicles are described and discussed, as well as current technical challenges to study them. RECENT FINDINGS: So far, only a few studies have addressed extracellular vesicles of embryonic origin and explored their potential as biomarkers for embryo selection...
April 13, 2018: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29651940/proteomics-in-human-assisted-reproduction
#3
Mauro Bibancos De Rose, Mariana Moraes Piccolomini
Assisted reproduction techniques (ART) have significantly advanced since the first successful in vitro fertilization (IVF). However, most in vitro-produced embryos fail to implant. Key steps in ART are the correct infertility diagnosis, in order to manage the individual treatments, and the assessment of gamete and embryo viability, to identify the embryo with the best implantation potential. Non-invasive approaches to correctly diagnose infertility and to access embryo development potential have the advantages of increasing the knowledge of embryo physiology, therefore allowing the development of methods to predict developmental competence and viability...
April 12, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29625985/importance-of-proteasome-gene-expression-during-model-dough-fermentation-after-freezing-preservation-of-baker-s-yeast-cells
#4
Daisuke Watanabe, Hiroshi Sekiguchi, Yukiko Sugimoto, Atsushi Nagasawa, Naotaka Kida, Hiroshi Takagi
Freeze-thaw stress causes various types of cellular damages, survival and/or proliferation defects, and metabolic alterations. However, the mechanisms underlying how cells cope with freeze-thaw stress are poorly understood. Here, model dough fermentations using two baker's yeast strains, 45 and YF, of Saccharomyces cerevisiae were compared after 2-week cell preservation in a refrigerator or freezer. YF exhibited slow fermentation after exposure to freeze-thaw stress due to the low cell viability. A DNA microarray analysis of the YF cells during fermentation revealed that the genes involved in oxidative phosphorylation were relatively strongly expressed, suggesting a decrease in the glycolytic capacity...
April 6, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29605407/next-generation-sequencing-for-preimplantation-genetic-screening-improves-pregnancy-outcomes-compared-with-array-comparative-genomic-hybridization-in-single-thawed-euploid-embryo-transfer-cycles
#5
Jenna Friedenthal, Susan M Maxwell, Santiago Munné, Yael Kramer, David H McCulloh, Caroline McCaffrey, James A Grifo
OBJECTIVE: To evaluate whether the use of next generation sequencing (NGS) for preimplantation genetic screening (PGS) in single thawed euploid embryo transfer (STEET) cycles improves pregnancy outcomes compared with array comparative genomic hybridization (aCGH). DESIGN: Retrospective cohort study. SETTING: Single university-based fertility center. PATIENT(S): A total of 916 STEET cycles from January 2014 to December 2016 were identified...
March 28, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29598846/the-role-of-mitochondrial-activity-in-female-fertility-and-assisted-reproductive-technologies-overview-and-current-insights
#6
REVIEW
Gustavo Nardini Cecchino, Emre Seli, Eduardo Leme Alves da Motta, Juan Antonio García-Velasco
Mitochondria have been implicated as key factors regulating female reproductive processes. Notable progress has been made in determining the role of mitochondria with respect to oocyte maturation, fertilization and early embryo development. In addition, mitochondrial function and dysfunction has been the subject of various studies in ovarian ageing and metabolic stress models. However, the overall mitochondrial impact on female fertility is yet to be uncovered. The mitochondrial DNA content of granulosa, cumulus and trophectoderm cells is being explored as a biomarker of oocyte quality and embryo viability...
March 8, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29567383/eventual-re-vitrification-or-storage-in-liquid-nitrogen-vapor-does-not-jeopardize-the-practical-handling-and-transport-of-vitrified-pig-embryos
#7
Alicia Nohalez, Cristina A Martinez, Inmaculada Parrilla, Carolina Maside, Jordi Roca, María A Gil, Heriberto Rodriguez-Martinez, Emilio A Martinez, Cristina Cuello
This study aimed (1) to evaluate the in vitro post-warming survival of porcine embryos after re-vitrification and (2) to assess the efficacy of transport of embryos in dry shipper (DS) in maintaining the viability and quality of vitrified embryos for a 3-day period. Embryos at the compacted or cavitating morula (CCM) and unhatched blastocyst (UBL) stages were surgically obtained from weaned, crossbred sows. In the first experiment, more than 85% of the embryos survived an initial vitrification and warming and achieved comparable survival rates to those of their fresh counterparts...
March 8, 2018: Theriogenology
https://www.readbyqxmd.com/read/29564763/embryonary-mouse-cardiac-fibroblast-isolation
#8
Alejandra Garate-Carrillo, Israel Ramirez
Mouse cardiac fibroblasts have been widely used as an in vitro model for studying fundamental biological processes and mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Cardiac FBs are relatively easy to culture in a dish and can be manipulated using molecular and pharmacological tools. Because FBs rapidly decrease cell cycle division and proliferative rate after birth, they are prone to phenotypic changes and senescence in cell culture soon after a few passages...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29547689/metabolomics-for-improving-pregnancy-outcomes-in-women-undergoing-assisted-reproductive-technologies
#9
REVIEW
Charalampos S Siristatidis, Eleni Sertedaki, Dennis Vaidakis, Christos Varounis, Marialena Trivella
BACKGROUND: In order to overcome the low effectiveness of assisted reproductive technologies (ART) and the high incidence of multiple births, metabolomics is proposed as a non-invasive method to assess oocyte quality, embryo viability, and endometrial receptivity, and facilitate a targeted subfertility treatment. OBJECTIVES: To evaluate the effectiveness and safety of metabolomic assessment of oocyte quality, embryo viability, and endometrial receptivity for improving live birth or ongoing pregnancy rates in women undergoing ART, compared to conventional methods of assessment...
March 16, 2018: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29546326/a-rationale-for-biopsying-embryos-reaching-the-morula-stage-on-day-6-in-women-undergoing-preimplantation-genetic-testing-for-aneuploidy
#10
M Irani, N Zaninovic, C Canon, C O'Neill, V Gunnala, Q Zhan, G Palermo, D Reichman, Z Rosenwaks
STUDY QUESTION: Is there a benefit to assessing ploidy in delayed embryos reaching the morula stage on Day 6 of development? SUMMARY ANSWER: Day-6 morulae should be considered for biopsy in women <40 years old undergoing preimplantation genetic testing for aneuploidy (PGT-A) because they are associated with acceptable, albeit reduced, euploidy and implantation rates (IRs). WHAT IS KNOWN ALREADY: Embryo development and morphology have been shown to correlate with aneuploidy and pregnancy rates...
March 13, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29543848/physiological-oxygen-culture-reveals-retention-of-metabolic-memory-in-human-induced-pluripotent-stem-cells
#11
Alexandra J Harvey, Carmel O'Brien, Jack Lambshead, John R Sheedy, Joy Rathjen, Andrew L Laslett, David K Gardner
Reprogramming somatic cells to a pluripotent cell state (induced Pluripotent Stem (iPS) cells) requires reprogramming of metabolism to support cell proliferation and pluripotency, most notably changes in carbohydrate turnover that reflect a shift from oxidative to glycolytic metabolism. Some aspects of iPS cell metabolism differ from embryonic stem (ES) cells, which may reflect a parental cell memory, or be a consequence of the reprogramming process. In this study, we compared the metabolism of 3 human iPS cell lines to assess the fidelity of metabolic reprogramming...
2018: PloS One
https://www.readbyqxmd.com/read/29542357/treating-donor-cells-with-2-pcpa-corrects-aberrant-histone-h3k4-dimethylation-and-improves-cloned-goat-embryo-development
#12
Tingchao Mao, Chengquan Han, Ruizhi Deng, Biao Wei, Peng Meng, Yan Luo, Yong Zhang
Epigenetic modifications extensively occur in mammalian embryonic development and cell differentiation process. They play an essential role in the reprogramming of nuclei during somatic cell nuclear transfer (SCNT) and subsequent in vitro embryonic development. Recently, SCNT embryos have been verified to contain a subnormal level of histone H3K4 dimethylation (H3K4me2) in contrast to in vitro fertilized embryos. This finding suggested that increasing H3K4me2 levels may ameliorate the aberrant development of cloned embryos...
March 15, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29540428/the-hidden-function-of-egg-white-antimicrobials-egg-weight-dependent-effects-of-avidin-on-avian-embryo-survival-and-hatchling-phenotype
#13
Eva Krkavcová, Jakub Kreisinger, Ludmila Hyánková, Pavel Hyršl, Veronika Javůrková
Avidin is key egg white antimicrobial protein with strong binding capacity for biotin, an essential growth and immune cell precursor. As such, it is assumed to have a pronounced, though still poorly explored, effect on hatchling phenotype. We tested the effect of experimentally increased egg white avidin concentration (AVIDIN+) on hatching success, chick morphology, post-hatching growth performance and innate immune function in a model bird, Japanese Quail ( Coturnix japonica ). Probability of embryo survival in the late embryonic phase increased with increasing egg weight in control eggs, but not in AVIDIN+ eggs...
March 14, 2018: Biology Open
https://www.readbyqxmd.com/read/29538677/critical-role-of-mtor-ppar%C3%AE-and-ppar%C3%AE-signaling-in-regulating-early-pregnancy-decidual-function-embryo-viability-and-feto-placental-growth
#14
Sabrina L Roberti, Romina Higa, Verónica White, Theresa L Powell, Thomas Jansson, Alicia Jawerbaum
STUDY QUESTION: What are the consequences of inhibiting mTOR, the mechanistic target of rapamycin, and the peroxisome proliferator activated receptor gamma (PPARγ) and PPARδ pathways in the early post-implantation period on decidual function, embryo viability and feto-placental growth in the rat? SUMMARY ANSWER: mTOR inhibition from day 7 to day 9 of pregnancy in rats caused decidual PPARγ and PPARδ upregulation on day 9 of pregnancy and resulted in embryo resorption by day 14 of pregnancy...
March 12, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29526734/combined-loss-of-jmjd1a-and-jmjd1b-reveals-critical-roles-for-h3k9-demethylation-in-the-maintenance-of-embryonic-stem-cells-and-early-embryogenesis
#15
Shunsuke Kuroki, Yuji Nakai, Ryo Maeda, Naoki Okashita, Mika Akiyoshi, Yutaro Yamaguchi, Satsuki Kitano, Hitoshi Miyachi, Ryuichiro Nakato, Kenji Ichiyanagi, Katsuhiko Shirahige, Hiroshi Kimura, Yoichi Shinkai, Makoto Tachibana
Histone H3 lysine 9 (H3K9) methylation is unevenly distributed in mammalian chromosomes. However, the molecular mechanism controlling the uneven distribution and its biological significance remain to be elucidated. Here, we show that JMJD1A and JMJD1B preferentially target H3K9 demethylation of gene-dense regions of chromosomes, thereby establishing an H3K9 hypomethylation state in euchromatin. JMJD1A/JMJD1B-deficient embryos died soon after implantation accompanying epiblast cell death. Furthermore, combined loss of JMJD1A and JMJD1B caused perturbed expression of metabolic genes and rapid cell death in embryonic stem cells (ESCs)...
February 27, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29513715/timing-of-embryonic-quiescence-determines-viability-of-embryos-from-the-calanoid-copepod-acartia-tonsa-dana
#16
Birgitte Nilsson, Benni Winding Hansen
Like 41 other calanoid copepods, Acartia tonsa, are capable of inducing embryonic quiescence when experiencing unfavorable environmental conditions. The ecdysone-signaling cascade is known to have a key function in developmental processes like embryogenesis and molting of arthropods, including copepods. We examined the role of ecdysteroid-phosphate phosphatase (EPPase), ecdysone receptor (EcR), ß fushi tarazu transcription factor 1 (ßFTZ-F1), and the ecdysteroid-regulated early gene E74 (E74), which represent different levels of the ecdysone-signaling cascade in our calanoid model organism...
2018: PloS One
https://www.readbyqxmd.com/read/29505797/changes-in-gene-expression-following-long-term-in-vitro-exposure-of-macaca-mulatta-trophoblast-stem-cells-to-biologically-relevant-levels-of-endocrine-disruptors
#17
Uros Midic, Benjamin Goheen, Kailey A Vincent, Catherine A VandeVoort, Keith E Latham
Trophoblast stem cells (TSCs) are crucial for embryo implantation and placentation. Environmental toxicants that compromise TSC function could impact fetal viability, pregnancy, and progeny health. Understanding the effects of low, chronic EDC exposures on TSCs and pregnancy is a priority in developmental toxicology. Differences in early implantation between primates and other mammals make a nonhuman primate model ideal. We examined effects of chronic low-level exposure to atrazine, tributyltin, bisphenol A, bis(2-ethylhexyl) phthalate, and perfluorooctanoic acid on rhesus monkey TSCs in vitro by RNA sequencing...
March 2, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/29505796/2-3-7-8-tetrachlorodibenzo-p-dioxin-can-alter-the-sex-ratio-of-embryos-with-decreased-viability-of-y-spermatozoa-in-mice
#18
Young-Ah You, Elsayed A Mohamed, Md Saidur Rahman, Woo-Sung Kwon, Won-Hee Song, Buom-Yong Ryu, Myung-Geol Pang
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a reproductive and developmental toxicant that can alter the sex ratio of offspring (proportion of male offspring). We hypothesized that the alteration of sex ratio is associated with sex chromosome ratio of live spermatozoa affected by exposure to TCDD. After exposure to TCDD we analyzed simultaneously sperm sex chromosome constitution and viability, and evaluated sperm sex chromosome ratio association with embryo sex ratio in mice. Short-term exposure to TCDD affects the decreased sperm motility and viability, and the increased acrosome reaction...
March 2, 2018: Reproductive Toxicology
https://www.readbyqxmd.com/read/29501817/manipulation-of-vegf-induced-angiogenesis-by-2-n-6-o-sulfated-chitosan
#19
Yuanman Yu, Rui Chen, Yi Sun, Yuanzhong Pan, Wei Tang, Shuang Zhang, Lingyan Cao, Yuan Yuan, Jing Wang, Changsheng Liu
Emerging evidence suggests that vascular endothelial growth factor (VEGF) is important in the treatment of various ischemic and cardiovascular diseases. However, it often suffers from high cost and easy deactivation with a short half-life. Here, we describe a synthetic 2-N, 6-O-sulfated chitosan (26SCS) with a high affinity to VEGF promoting the binding of the signaling protein to its VEGF receptor 2 (VEGFR2), activating receptor phosphorylation and pro-angiogenic related genes expression, and further stimulating downstream VEGF-dependent endothelial cell viability, migration, tube formation and rat aortic rings outgrowth...
March 1, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29499763/modulatory-effects-of-trophoblast-secreted-cxcl12-on-the-migration-and-invasion-of-human-first-trimester-decidual-epithelial-cells-are-mediated-by-cxcr4-rather-than-cxcr7
#20
Jiayi Zheng, Haiping Wang, Wenhui Zhou
BACKGROUND: Maternal-fetal crosstalk during embryo implantation is complex and regulated by local signaling molecules. Chemokines and their receptors are critical signaling components required for implantation and the process of pregnancy. This study aimed to explore whether human first-trimester trophoblast cells (TCs) were capable of modulating the migration and invasion of human first-trimester decidual epithelial cells (DECs) via CXCL12/CXCR4/CXCR7 signaling. METHOD: The expression of CXCR4 and CXCR7 in DECs was examined by immunohistochemistry, immunocytochemistry, immunofluorescence, flow cytometry, real-time polymerase chain reactions and western blotting...
March 2, 2018: Reproductive Biology and Endocrinology: RB&E
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