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Embryo viability

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https://www.readbyqxmd.com/read/28650975/defective-glucocorticoid-receptor-signaling-and-keratinocyte-autonomous-defects-contribute-to-skin-phenotype-of-mouse-embryos-lacking-the-hsp90-co-chaperone-p23
#1
Marta Madon-Simon, Iwona Grad, Pilar Bayo, Paloma Pérez, Didier Picard
p23 is a small acidic protein with intrinsic molecular chaperone activity. It is best known as a co-chaperone of the major cytosolic molecular chaperone Hsp90. p23 binds the N-terminus of Hsp90 and stabilizes the ATP-bound and N-terminally closed Hsp90 dimer. It is in this configuration that many Hsp90 clients are most stably bound. Considering the important role of p23 in the Hsp90 cycle, it came as a surprise that it is not absolutely essential for viability in the budding yeast or for mouse development. Mice without p23 develop quite normally until birth and then all die perinatally because of immature lungs...
2017: PloS One
https://www.readbyqxmd.com/read/28649667/impact-of-protein-kinase-ck2-inhibitors-on-proliferation-and-differentiation-of-neural-stem-cells
#2
Melanie Bender, Lisa Schwind, David Grundmann, Monika Martin, Markus Klotz, Claudia Götz, Mathias Montenarh, Karl-Herbert Schäfer
BACKGROUND: Protein kinases play central roles in cell and tissue development. Protein kinase CK2, an ubiquitously expressed serine/threonine kinase has severe impacts on embryo- and spermatogenesis. Since its role in neurogenesis has so far only been investigated in very few studies, we analysed the role of CK2 in neural stem cells by using two specific inhibitors. METHODS: Neural stem cells were isolated from the subventricular zone of neonatal mice, using a neurosphere approach...
June 2017: Heliyon
https://www.readbyqxmd.com/read/28645083/graphene-oxide-nanosheets-induce-dna-damage-and-activate-the-base-excision-repair-ber-signaling-pathway-both-in%C3%A2-vitro-and-in%C3%A2-vivo
#3
Chun-Jiao Lu, Xue-Feng Jiang, Muhammad Junaid, Yan-Bo Ma, Pan-Pan Jia, Hua-Bin Wang, De-Sheng Pei
Graphene oxide (GO) has widespread concerns in the fields of biological sciences and medical applications. Currently, studies have reported that excessive GO exposure can cause cellular DNA damage through reactive oxygen species (ROS) generation. However, DNA damage mediated response of the base excision repair (BER) pathway due to GO exposure is not elucidated yet. Therefore, we exposed HEK293T cells and zebrafish embryos to different concentrations of GO for 24 h, and transcriptional profiles of BER pathway genes, DNA damage, and cell viability were analyzed both in vitro and in vivo...
June 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28637492/expression-patterns-of-fshd-causing-dux4-and-myogenic-transcription-factors-pax3-and-pax7-are-spatially-distinct-in-differentiating-human-stem-cell-cultures
#4
Premi Haynes, Kelly Kernan, Suk-Lin Zhou, Daniel G Miller
BACKGROUND: Facioscapulohumeral muscular dystrophy (FSHD) is most commonly inherited in an autosomal dominant pattern and caused by the abnormal expression of DUX4 in skeletal muscle. The DUX4 transcription factor has DNA binding domains similar to several paired class homeotic transcription factors, but only myogenic factors PAX3 and PAX7 rescue cell viability when co-expressed with DUX4 in mouse myoblasts. This observation suggests competition for DNA binding sites in satellite cells might limit muscle repair and may be one aspect of DUX4-associated myotoxicity...
June 21, 2017: Skeletal Muscle
https://www.readbyqxmd.com/read/28623802/developmental-toxicity-of-glycine-coated-silica-nanoparticles-in-embryonic-zebrafish
#5
Eduard Dumitrescu, Dinusha P Karunaratne, Morgan K Prochaska, Xiaobo Liu, Kenneth N Wallace, Silvana Andreescu
Nanoparticle (NP) surface coatings are known to influence the toxicity of engineered nanomaterials. This work examines the effect of glycine functionalization on silica NPs and investigates changes in viability and developmental defects in the organs of zebrafish embryos upon exposure. Silica NPs and glycine-functionalized silica NPs are synthesized and characterized. Exposure of zebrafish embryos to glycine-silica NPs affects the mortality percentage in a similar manner to soluble glycine. Developmental defects are observed in embryos exposed to soluble glycine, glycine-silica NPs, or silica NPs in comparison with the unexposed embryos...
June 14, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28623660/the-method-of-coating-fe%C3%A2-o%C3%A2-with-carbon-nanoparticles-to-modify-biological-properties-of-oxide-measured-in-vitro
#6
Tomasz Niemiec, Mariusz Dudek, Natalia Dziekan, Sławomir Jaworski, Aleksandra Przewozik, Emilia Soszka, Anna Koperkiewicz, Piotr Koczoń
The coating of nanoparticles on materials for medical application [e.g., the coating of Fe₃O₄ nanopowder (IONP) with a carbon nanolayer] serves to protect and modify the selected biological, physical, and chemical properties of the coated material. Increases in chemical stability, changes in biocompatibility, and a modified surface structure are examples of the effects caused by the formation of carbon coatings. In the current study, Fe₃O₄ nanoparticles were coated with a carbon nanolayer (IONP@C) in a plasmochemical reactor (using radio-frequency plasma-enhanced chemical vapor deposition methods) under various experimental conditions...
June 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28610639/impact-of-the-ivf-laboratory-environment-on-human-preimplantation-embryo-phenotype
#7
D K Gardner, R L Kelley
The phenotype of the human embryo conceived through in vitro fertilization (IVF), that is its morphology, developmental kinetics, physiology and metabolism, can be affected by numerous components of the laboratory and embryo culture system (which comprise the laboratory environment). The culture media formulation is important in determining embryo phenotype, but this exists within a culture system that includes oxygen, temperature, pH and whether an embryo is cultured individually or in a group, all of which can influence embryo development...
June 14, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28601158/effect-of-corifollitropin-alfa-supplemented-with-or-without-lh-on-ovarian-stimulation-and-embryo-viability-in-rabbit
#8
María Pilar Viudes-de-Castro, Francisco Marco-Jiménez, José Isaí Cedano-Castro, José Salvador Vicente
There is increasing interest in using rabbits for research as a laboratory model as well as for industrial production of meat, wool and fur. Superovulation in animals is used to produce a maximum number of transferable embryos per donor, in order to either support genetic improvement programs, ex situ conservation or to optimize other biotechnologies. Over time, the use of this biotechnology has shown variable outcomes as a consequence of several factors, such as the origin of exogenous hormone, posology and the effect of gonadotropins used simultaneously, the donor and the environment...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28598819/newcastle-disease-virus-induced-autophagy-mediates-antiapoptotic-signaling-responses-in-vitro-and-in-vivo
#9
Yinfeng Kang, Runyu Yuan, Bin Xiang, Xiaqiong Zhao, Pei Gao, Xu Dai, Ming Liao, Tao Ren
In this study, we investigated the role of autophagy and apoptosis in Newcastle disease virus (NDV)-infected chicken cells and tissues. NDV-infected and starvation-induced chick embryo fibroblasts (CEF) cells showed higher autophagosome formation than mock-infected CEF cells on transmission electron microscopy. The NDV-infected CEF cells showed enhanced conversion of microtubule-associated protein 1 light chain 3-I (LC3-I) to LC3-II and degradation of p62/SQSTM1. The diminished conversion of LC3-I to LC3-II and cleaved caspase 3 and poly (ADP-ribose) polymerase (PARP) in ultraviolet-inactivated NDV-infected cells suggested that autophagosome formation was necessary for NDV replication...
May 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28590029/treatment-of-heterotopic-caesarean-scar-pregnancy-complicated-with-post-termination-increase-in-size-of-residual-mass-and-morbidly-adherent-placenta
#10
Andre Hadyme Miyague, Adriano Pienaro Chrisostomo, Sergio Luis Costa, Edson Tetsuya Nakatani, William Kondo, Claudio Corrêa Gomes
We report a case of a secundipara with heterotopic cesarean scar pregnancy (HCSP) treated with potassium chloride injection into the ectopic embryo followed by sac aspiration. The remaining "mass" increased in size threefold and was surrounded by a rich vascular network. An arteriovenous malformation was suspected; however, appropriate treatment was precluded because of the viability of the ectopic gestation. Sonographic examination revealed a morbidly adherent placenta, and attempt to resect the mass laparoscopically was complicated by bleeding that required hysterectomy...
June 7, 2017: Journal of Clinical Ultrasound: JCU
https://www.readbyqxmd.com/read/28576684/influence-of-oil-related-environmental-pollutants-on-female-reproduction
#11
REVIEW
Alexander V Sirotkin, Abdul Halim Harrath
The petroleum low-weight aromatic hydrocarbons benzene, toluene, ethylbenzene, m/p-xylene, and o-xylene, also known as BTEX, are among the most common hazardous sources of environmental contamination. This paper reviews the available data concerning the effects of BTEX on different aspects of female reproduction, including the fecundity, ovaries, central nervous system (CNS), oocytes, embryos, oviducts, cytogenetics of somatic and generative cells, intracellular signaling systems, and hypothalamic, pituitary and peripheral reproductive hormones...
May 31, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28574506/role-of-atg5-dependent-cell-death-in-the-embryonic-development-of-bax-bak-double-knockout-mice
#12
Satoko Arakawa, Masatsune Tsujioka, Tatsushi Yoshida, Hajime Tajima-Sakurai, Yuya Nishida, Yosuke Matsuoka, Ikuyo Yoshino, Yoshihide Tsujimoto, Shigeomi Shimizu
Programmed cell death, which is required for the development and homeostasis of metazoans, includes mechanisms such as apoptosis, autophagic cell death, and necrotic (or type III) death. Members of the Bcl2 family regulate apoptosis, among which Bax and Bak act as a mitochondrial gateway. Although embryonic fibroblasts from Bax/Bak double-knockout (DKO) mice are resistant to apoptosis, we previously demonstrated that these cells die through an autophagy-dependent mechanism in response to various types of cellular stressors...
June 2, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28552887/selection-of-viable-in-vitro-fertilized-bovine-embryos-using-time-lapse-monitoring-in-microwell-culture-dishes
#13
Satoshi Sugimura, Tomonori Akai, Kei Imai
Conventionally, in vitro-fertilized (IVF) bovine embryos for transfer are morphologically evaluated at day 7-8 of embryo culture. This method is, however, subjective and results in unreliable selection. We previously described a novel selection system for IVF bovine blastocysts for transfer that traces the development of individual embryos with time-lapse monitoring in our specially developed microwell culture dishes (LinKID micro25). The system can noninvasively identify prognostic factors that reflect viability after transfer...
May 26, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28551726/embryo-density-may-affect-embryo-quality-during-in-vitro-culture-in-a-microwell-group-culture-dish
#14
Adam Lehner, Zita Kaszas, Akos Murber, Janos Rigo, Janos Urbancsek, Peter Fancsovits
PURPOSE: Culturing embryos in groups is a common practice in mammalian embryology. Since the introduction of different microwell dishes, it is possible to identify oocytes or embryos individually. As embryo density (embryo-to-volume ratio) may affect the development and viability of the embryos, the purpose of this study was to assess the effect of different embryo densities on embryo quality. METHODS: Data of 1337 embryos from 228 in vitro fertilization treatment cycles were retrospectively analyzed...
May 27, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28545340/optimized-methods-for-rapidly-dissecting-spinal-cords-and-harvesting-spinal-motor-neurons-with-high-survival-and-purity-from-rats-at-different-embryonic-stages
#15
Shudong Chen, Ruimin Tian, Hui Li, Meihui Chen, Hu Zhang, Dingkun Lin
STUDY DESIGN: Experimental study, protocol optimization. OBJECTIVES: To investigate and compare the isolation of spinal motor neurons from embryonic rats at different embryonic stages, and develop optimized methods for rapidly dissecting spinal cords and harvesting spinal motor neurons with high survival and purity. SETTING: Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China. METHODS: Embryonic rats at different embryonic stages (12-18 days) were used to isolate spinal motor neurons...
May 25, 2017: Journal of Spinal Cord Medicine
https://www.readbyqxmd.com/read/28534597/metabolomics-for-improving-pregnancy-outcomes-in-women-undergoing-assisted-reproductive-technologies
#16
REVIEW
Charalampos S Siristatidis, Eleni Sertedaki, Dennis Vaidakis
BACKGROUND: In order to overcome the low effectiveness of assisted reproductive technologies (ART) and the high incidence of multiple births, metabolomics is proposed as a non-invasive method to assess oocyte quality, embryo viability, and endometrial receptivity, and facilitate a targeted subfertility treatment. OBJECTIVES: To evaluate the effectiveness and safety of metabolomic assessment of oocyte quality, embryo viability, and endometrial receptivity for improving live birth or ongoing pregnancy rates in women undergoing ART, compared to conventional methods of assessment...
May 23, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28534057/the-effect-of-thawing-protocols-on-follicle-conservation-in-human-ovarian-tissue-cryopreservation
#17
T Morewood, N Getreu, B Fuller, J Morris, P Hardiman
BACKGROUND: Ovarian tissue cryopreservation has the potential to improve fertility preservation for a growing number of patients undergoing sterilising therapy, particularly where oocyte or embryo cryopreservation is not suitable. However, its success is limited by significant follicular apoptosis upon thawing, and there is wide variation in thawing protocols used with little evidence of efficacy. OBJECTIVE: To determine the best warming rates to maintain tissue viability...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28534051/cooling-strategies-for-brazilian-flounder-paralichthys-orbignyanus-embryos
#18
A S Varela, T F Cardoso, E Fernandes E Silva, K L Goularte, M H Okamoto, L A Sampaio, R D Jardim, C D Corcini
BACKGROUND: Paralichthys orbignyanus is the species of the greatest potential for marine and estuarine fish farming in southern Brazil. Consequently, embryo cryopreservation becomes an important tool for increasing their production. OBJECTIVE: To evaluate the effects of cooling protocols on the viability of embryos of P. orbignyanus at two stages of development (neurula and early differentiation of the tail). MATERIALS AND METHODS: Control embryos were maintained at 23 degree C and treated embryos were cooled to 15 degree C, 10 degree C and 5 degree C at rapid, moderate and slow cooling rates...
March 2017: Cryo Letters
https://www.readbyqxmd.com/read/28533817/mosaicism-for-structural-non-centromeric-autosomal-rearrangements-in-disease-defined-carriers-sex-differences-in-the-rearrangements-profile-and-maternal-age-distributions
#19
REVIEW
Natalia V Kovaleva, Philip D Cotter
BACKGROUND: Mosaicism for an autosomal structural rearrangement (Rea) associated with clinical manifestation of chromosomal imbalance is rare. Consequently, there is a lack of basic epidemiological characterization of this kind of mosaicism, such as population rate, cytogenetic profile of Reas involved, maternal age distribution, and sex (male to female) ratio among Rea carriers. The objectives of the present study were: (i) determination of the Rea profile in clinically affected individuals, (ii) comparative analysis of the cytogenetic profile and involvement of single chromosomes to rearrangements in affected and previously reported asymptomatic carriers, (iii) analysis of the male/female ratio in carriers of various types of Rea, and, (iv) examination of parental ages distributions according to carriers' sex...
2017: Molecular Cytogenetics
https://www.readbyqxmd.com/read/28532827/hepatoma-derived-growth-factor-protein-quantification-in-uterine-fluid-gene-expression-in-endometrial-cell-culture-and-effects-on-in%C3%A2-vitro-embryo-development-pregnancy-and-birth
#20
E Gómez, S Carrocera, D Martin, M J Sánchez-Calabuig, A Gutiérrez-Adán, A Murillo, M Muñoz
Hepatoma-derived growth factor (HDGF) is present in the endometrium of cows and other mammals. Recombinant HDGF (rHDGF) improves bovine blastocyst development in vitro. However, specific culture conditions and essential aspects of HDGF uterine physiology are yet unknown. In this work we quantified total HDGF protein in uterine fluid (UF) by multiple reaction monitoring (MRM), and analyzed effects of rHDGF on specific embryonic stages with Day-6 bovine embryos cultured in vitro with and without BSA, and on pregnancy viability and calf phenotypes after embryo transfer to recipients...
July 1, 2017: Theriogenology
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