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Embryo viability

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https://www.readbyqxmd.com/read/29240896/dechorionation-and-permeabilization-of-podisus-nigrispinus-heteroptera-pentatomidae-eggs-limiting-factors-for-cryopreservation
#1
Ítala Tainy Barreto Francisco Dos Santos, Júlio César Melo Poderoso, Edson José Santana Dos Santos, Genésio Tâmara Ribeiro, Jamile Fernanda Silva Cossolin, José Eduardo Serrão
Cryopreservation protocols have been developed for eggs of Diptera, Lepidoptera, and Hymenoptera; however, for Heteroptera, such as Podisus nigrispinus Dallas, 1851 (Pentatomidae), no procedures have been described yet. The objective of this study was to evaluate the processes of dechorionation and permeabilization on the viability of eggs of P. nigrispinus with different embryonic ages. In the laboratory, embryos of 24, 48, 72, and 96 h of age were submitted to sodium hydroxide, sodium hypochlorite, isopropyl, and hexane solutions for dechorionation and permeabilization...
December 12, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29233229/effects-of-pi3k-and-fsh-on-steroidogenesis-viability-and-embryo-development-of-the-cumulus-oocyte-complex-after-in-vitro-culture
#2
Danielle Kaiser de Souza, Loise Pedrosa Salles, Ricardo Camargo, Laura Vanessa Mourão Gulart, Suellen Costa E Silva, Beatriz Dolabela de Lima, Fernando Araripe Gonçalves Torres, Alzira Amélia Martins Rosa E Silva
The purpose of this study was to evaluate the effects of FSH and PI3K on the nuclear maturation, viability, steroidogenesis and embryo development of bovine cumulus-oocyte complexes (COCs). Oocyte maturation was achieved with MIV B, MIV B+100 µM LY294002, MIV B+10 ng/mL follicle stimulating hormone (FSH), or MIV B+10 ng/mL FSH+100 µM LY294002 treatments for 22-24 h. After the cultured COCs were denuded, oocytes were separated into those that extruded polar bodies (mature) and those that did not, and real-time polymerase chain reaction (PCR) for BAX, BCL2, LHR, FSHR, CYP11A1, CYP19A1 and HSD17B1 genes was performed...
December 13, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29232876/evaluation-of-fibrin-based-interpenetrating-polymer-networks-as-potential-biomaterials-for-tissue-engineering
#3
Olfat Gsib, Jean-Luc Duval, Mathieu Goczkowski, Marie Deneufchatel, Odile Fichet, Véronique Larreta-Garde, Sidi Ahmed Bencherif, Christophe Egles
Interpenetrating polymer networks (IPNs) have gained great attention for a number of biomedical applications due to their improved properties compared to individual components alone. In this study, we investigated the capacity of newly-developed naturally-derived IPNs as potential biomaterials for tissue engineering. These IPNs combine the biologic properties of a fibrous fibrin network polymerized at the nanoscale and the mechanical stability of polyethylene oxide (PEO). First, we assessed their cytotoxicity in vitro on L929 fibroblasts...
December 10, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29232641/effect-of-thiacloprid-on-early-life-stages-of-common-carp-cyprinus-carpio
#4
Josef Velisek, Alzbeta Stara
Toxicity of the neonicotinoid pesticide thiacloprid to embryos and larvae of common carp Cyprinus carpio was assessed under laboratory conditions. Carp embryos and larvae were exposed to thiacloprid at four concentrations: 4.5 (environmental concentration), 45, 225, and 450 μg/L for 35 days and compared to a non-treated control group. The effects on lipid peroxidation, antioxidant enzyme levels (superoxide dismutase, glutathione reductase, catalase, glutathione S-transferase, and reduced glutathione), mortality, growth, development, and histopathogenesis were assessed...
December 1, 2017: Chemosphere
https://www.readbyqxmd.com/read/29229552/caspase-6-is-a-dispensable-enabler-of-adult-mammalian-axonal-degeneration
#5
V Woo, C Cheng, A Duraikannu, A Chandrasekhar, K Purdy, J A Martinez, D W Zochodne
The progress of axonal degeneration (AxD) following injury or insult impacts both recovery from axonal transection and protection of axons from diverse insults, or axonopathy. Here we provide evidence that increases in capase-6 (Casp6) expression and action contribute to the progression of AxD. The expression of Casp6 protein and mRNA in distal branches of sensory axons undergoing AxD was confirmed. We developed and utilized a new model of axonopathy in live mice by serially visualizing the viability of cutaneous axons in the ear pinna that expressed an axonal YFP transgene, in response to capasaicin-induced AxD...
December 8, 2017: Neuroscience
https://www.readbyqxmd.com/read/29226264/generation-of-viable-progeny-from-dead-brooders-of-endangered-catfish-clarias-magur-hamilton-1822
#6
Sullip Kumar Majhi, Santosh Kumar
The obligatory air-breathing catfish Clarias magur is a prime candidate for aquaculture owing to its unique taste, high growth rate, and hardy nature. However, recently the IUCN has listed the species under the endangered category because the population has critically declined in the wild. The sexually mature C. magur brooders are often collected from their natural habitats for seed production in captivity. In many cases, the brooder dies due to handling injuries or confinement stress. In this study, we demonstrated that viable progeny could be generated from freshly dead sexually mature C...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29209446/oxidative-stress-alters-the-profile-of-transcription-factors-related-to-early-development-on-in-vitro-produced-embryos
#7
Roberta Ferreira Leite, Kelly Annes, Jessica Ispada, Camila Bruna de Lima, Érika Cristina Dos Santos, Patricia Kubo Fontes, Marcelo Fábio Gouveia Nogueira, Marcella Pecora Milazzotto
High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development. For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%)...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29207159/downregulation-of-mir%C3%A2-146a-promotes-proliferation-and-migration-of-aob%C3%A2-treated-embryoid-body-via-pdgfra-induction
#8
Lejing Li, Shoulian Zhu, Ying Li, Wenyuan Cao, Xiaoli Qiao
Antioxidant of bamboo leaves (AOB) has been proven to have antioxidant activity and an inhibitory effect on free radicals that induce deterioration of macromolecules. The multi‑target regulation of microRNAs (miRs) in the complicated process of vasculogenesis and angiogenesis lead to the use of miRNA therapy in vascular development. In the present study, the role of miRNAs on early embryo vascular development upon AOB stimulation was investigated. For this purpose, mouse embryonic stem cells were spontaneously differentiated as embryoid bodies (EBs) and were examined by phase contrast microscopy...
November 22, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29202392/serial-hcg-and-progesterone-levels-to-predict-early-pregnancy-outcomes-in-pregnancies-of-uncertain-viability-a-prospective-study
#9
Claire Puget, Yolaine Joueidi, Estelle Bauville, Bruno Laviolle, Claude Bendavid, Vincent Lavoué, Maela Le Lous
OBJECTIVE: To assess the value of serial hCG and progesterone serum level in the diagnosis of early pregnancy viability. METHODS: It was a prospective cohort study. Women with a pregnancy of uncertain viability (PUV), defined as the presence of an intra-uterine embryo with a crown-rump length <7mm with no cardiac activity or an intra-uterine gestational sac size <25mm with no visible embryonic structure in a transvaginal ultrasound scan (TVS) were eligible...
December 1, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29188109/speckle-variance-oct-for-depth-resolved-assessment-of-the-viability-of-bovine-embryos
#10
S Caujolle, R Cernat, G Silvestri, M J Marques, A Bradu, T Feuchter, G Robinson, D K Griffin, A Podoleanu
The morphology of embryos produced by in vitro fertilization (IVF) is commonly used to estimate their viability. However, imaging by standard microscopy is subjective and unable to assess the embryo on a cellular scale after compaction. Optical coherence tomography is an imaging technique that can produce a depth-resolved profile of a sample and can be coupled with speckle variance (SV) to detect motion on a micron scale. In this study, day 7 post-IVF bovine embryos were observed either short-term (10 minutes) or long-term (over 18 hours) and analyzed by swept source OCT and SV to resolve their depth profile and characterize micron-scale movements potentially associated with viability...
November 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/29180568/maternal-nanog-is-critical-for-the-zebrafish-embryo-architecture-and-for-cell-viability-during-gastrulation
#11
Marina Veil, Melanie Anna Schaechtle, Meijiang Gao, Viola Kirner, Lenka Buryanova, Rachel Grethen, Daria Onichtchouk
Nanog has been implicated in establishment of pluripotency in mammals and in zygotic genome activation in zebrafish. In this study we characterize the development of maternal and zygotic MZnanog null mutant zebrafish embryos. Without functional Nanog, epiboly is severely affected, embryo axes do not form and massive cell death starts at the end of gastrulation. We show that three independent defects in MZnanog mutants contribute to epiboly failure: yolk microtubule organization required for epiboly is abnormal, maternal mRNA fails to degrade due to the absence of miR-430 and actin structure of the yolk syncytial layer does not form properly...
November 27, 2017: Development
https://www.readbyqxmd.com/read/29176605/mechanistic-insight-to-ros-and-apoptosis-regulated-cytotoxicity-inferred-by-green-synthesized-cuo-nanoparticles-from-calotropis-gigantea-to-embryonic-zebrafish
#12
Puja Kumari, Pritam Kumar Panda, Ealisha Jha, Khushboo Kumari, Kumari Nisha, M Anwar Mallick, Suresh K Verma
With the rapid development of nanotechnology, much has been anticipated with copper oxide nanoparticles (CuO NP) due to their extensive industrial and commercial application. However, it has raised concern over the environmental safety and human health effects. In this study, CuO nanoparticles were synthesized using the green method with floral extract of Calotropis gigantea and characterized by standard physiochemical techniques like DLS, Zeta potential determination, UV- Visible Spectroscopy, XRD, FTIR, FESEM, and TEM...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29175537/vitrification-of-human-pronuclear-oocytes-by-direct-plunging-into-cooling-agent-non-sterile-liquid-nitrogen-vs-sterile-liquid-air
#13
Vladimir Isachenko, Plamen Todorov, Akerke Seisenbayeva, Yerzhan Toishibekov, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Dolores Foth, Markus Merzenich
In fact, a full sterilization of commercially-produced liquid nitrogen contaminated with different pathogens is not possible. The aim of this study was to compare the viability of human pronuclear oocytes subjected to cooling by direct submerging of open carrier in liquid nitrogen versus submerging in clean liquid air (aseptic system). One- and three-pronuclei stage embryos (n = 444) were cryopreserved by direct plunging into liquid nitrogen (vitrified) in ethylene glycol (15%), dimethylsulphoxide (15%) and 0...
November 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/29171438/in-vitro-neuroprotective-effects-of-ciliary-neurotrophic-factor-on-dorsal-root-ganglion-neurons-with-glutamate-induced-neurotoxicity
#14
Shu-Yun Wen, Ai-Min Li, Kuan-Qing Mi, Rui-Zheng Wang, Hao Li, Hua-Xiang Liu, Yi Xing
Ciliary neurotrophic factor has neuroprotective effects mediated through signal transducer and Janus kinase (JAK) 2/activator of transcription 3 (STAT3) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathways. Whether ciliary neurotrophic factor is neuroprotective for glutamate-induced excitotoxicity of dorsal root ganglion neurons is poorly understood. In the present study, the in vitro neuroprotective effects of ciliary neurotrophic factor against glutamate-induced excitotoxicity were determined in a primary culture of dorsal root ganglion neurons from Wistar rat embryos at embryonic day 15...
October 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/29164747/a-role-for-fruit-structure-in-seed-survival-and-germination-of-swartzia-langsdorffii-raddi-beyond-dispersal
#15
T A A Vaz, A G Rodrigues-Junior, A C Davide, A T Nakamura, P E Toorop
Diaspore structure has been hypothesized to play a role in seed viability and/or germination of recalcitrant seeds, especially for Swartzia langsdorffii. Thus, this work aims to (1) investigate in situ contribution of pericarp and aril on seed viability and germination, and (2) identify morphoanatomical traits of Swartzia langsdorffii diaspores that aid its desiccation-sensitive seed to remain viable. The role of the pericarp and aril in seed survival and germination was investigated by placing the whole fruit, whole seeds (arillate seed) and bare seeds (without aril) on the soil in the forest understory, assessing germination, emergence, dead, firm and predated seeds; and water content (WC) of pericarps, arils and seeds...
November 22, 2017: Plant Biology
https://www.readbyqxmd.com/read/29162091/role-of-micrornas-in-embryo-implantation
#16
REVIEW
Jingjie Liang, Shaoyu Wang, Zhengguang Wang
Failure of embryo implantation is a major limiting factor in early pregnancy and assisted reproduction. Determinants of implantation include the embryo viability, the endometrial receptivity, and embryo-maternal interactions. Multiple molecules are involved in the regulation of implantation, but their specific regulatory mechanisms remain unclear. MicroRNA (miRNA), functioning as the transcriptional regulator of gene expression, has been widely reported to be involved in embryo implantation. Recent studies reveal that miRNAs not only act inside the cells, but also can be released by cells into the extracellular environment through multiple packaging forms, facilitating intercellular communication and providing indicative information associated with physiological and pathological conditions...
November 21, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29156349/biopolymer-gelatin-coated-zinc-oxide-nanoparticles-showed-high-antibacterial-antibiofilm-and-anti-angiogenic-activity
#17
Mani Divya, Baskaralingam Vaseeharan, Muthukumar Abinaya, Sekar Vijayakumar, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
The use of natural polymers in drug design plays an important role in biomedical applications. Combinations of nanoparticles (NPs) and biopolymers have been shown to be useful for many purposes. This study focused on gelatin-coated zinc oxide NPs synthesized by co-precipitation. The particles were characterized by UV-Vis spectrum, showing a main peak at 375nm. The stability and crystalline nature of the particles was evaluated by Zeta potential and X-ray diffraction analysis. Fourier transform infrared spectroscopy (FTIR) revealed the possible functional groups of Ge-ZnO NPs, with strong bands at 3851, 3447, and 2923cm(-1)...
November 10, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29155779/a-novel-use-of-three-dimensional-high-frequency-ultrasonography-for-early-pregnancy-characterization-in-the-mouse
#18
Mary C Peavey, Corey L Reynolds, Maria M Szwarc, William E Gibbons, Cecilia T Valdes, Francesco J DeMayo, John P Lydon
High-frequency ultrasonography (HFUS) is a common method to non-invasively monitor the real-time development of the human fetus in utero. The mouse is routinely used as an in vivo model to study embryo implantation and pregnancy progression. Unfortunately, such murine studies require pregnancy interruption to enable follow-up phenotypic analysis. To address this issue, we used three-dimensional (3-D) reconstruction of HFUS imaging data for early detection and characterization of murine embryo implantation sites and their individual developmental progression in utero...
October 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29152028/a-fast-and-efficient-size-separation-method-for-haploid-embryonic-stem-cells
#19
Remo Freimann, Anton Wutz
Hemizygous mutations introduced in haploid genomes can directly expose a phenotype, thus facilitating gene function analysis and forward genetic screening. Recently, mammalian haploid cells could be derived from mouse, rat, monkey, and human embryos and have been applied to screens of cellular mechanisms including cell signaling, pathogen host factors, and developmental pathways. Notably, haploid cell cultures have an intrinsic tendency for diploidization and, thus, require periodic cell sorting. Here, we report a method for rapid purification of haploid mouse embryonic stem cells from mixed cell populations with high viability and yield...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29149327/focused-time-lapse-analysis-reveals-novel-aspects-of-human-fertilization-and-suggests-new-parameters-of-embryo-viability
#20
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
November 15, 2017: Human Reproduction
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