keyword
https://read.qxmd.com/read/36241974/primedsherlock-a-tool-for-rapid-design-of-highly-specific-crispr-cas12-crrnas
#21
JOURNAL ARTICLE
James G Mann, R Jason Pitts
BACKGROUND: CRISPR-Cas based diagnostic assays provide a portable solution which bridges the benefits of qRT-PCR and serological assays in terms of portability, specificity and ease of use. CRISPR-Cas assays are rapidly fieldable, specific and have been rigorously validated against a number of targets, including HIV and vector-borne pathogens. Recently, CRISPR-Cas12 and CRISPR-Cas13 diagnostic assays have been granted FDA approval for the detection of SARS-CoV-2. A critical step in utilizing this technology requires the design of highly-specific and efficient CRISPR RNAs (crRNAs) and isothermal primers...
October 14, 2022: BMC Bioinformatics
https://read.qxmd.com/read/36007411/electrochemical-detection-of-zika-and-dengue-infections-using-a-single-chip
#22
JOURNAL ARTICLE
Isabella Sampaio, Felipe Domingues Quatroni, Juliana Naomi Yamauti Costa, Valtencir Zucolotto
Zika and Dengue are infectious diseases caused by flaviviruses and transmitted by Aedes mosquitoes. Although symptoms are usually mild, complications such as dengue hemorrhagic fever and microcephaly in newborns -after the pregnant woman becomes infected with the Zika virus-have emerged as a global public health concern. The co-circulation of Zika and Dengue viruses and the overlapping of their symptoms represent a challenge for the accurate diagnosis. A single test for the point-of-care detection of both diseases is crucial...
August 17, 2022: Biosensors & Bioelectronics
https://read.qxmd.com/read/35864917/seropositive-neuromyelitis-optica-in-a-case-of-undiagnosed-ankylosing-spondylitis-a-neuro-rheumatological-conundrum
#23
Ritwik Ghosh Md, Devlina Roy, Moisés León-Ruiz, Shambaditya Das, Souvik Dubey, Julián Benito-León
Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune astrocytopathy against foot processes of aquaporin-4 (AQP4) water channels. Patients with NMOSD tend to have other coexisting autoimmune/connective tissue diseases. However, AQP-4-antibody-positive NMOSD coexisting with ankylosing spondylitis (AS) is rare. AS is an immune-mediated disorder, a subset of axial spondyloarthropathies, which commonly manifests as chronic inflammatory back pain in young people, and it has a strong association with HLA-B27...
2022: Qatar Medical Journal
https://read.qxmd.com/read/35746172/near-infrared-spectroscopy-as-a-potential-covid-19-early-detection-method-a-review-and-future-perspective
#24
REVIEW
Muna E Raypah, Asma Nadia Faris, Mawaddah Mohd Azlan, Nik Yusnoraini Yusof, Fariza Hanim Suhailin, Rafidah Hanim Shueb, Irneza Ismail, Fatin Hamimi Mustafa
The coronavirus disease 2019 (COVID-19) pandemic is a worldwide health anxiety. The rapid dispersion of the infection globally results in unparalleled economic, social, and health impacts. The pathogen that causes COVID-19 is known as a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A fast and low-cost diagnosis method for COVID-19 disease can play an important role in controlling its proliferation. Near-infrared spectroscopy (NIRS) is a quick, non-destructive, non-invasive, and inexpensive technique for profiling the chemical and physical structures of a wide range of samples...
June 10, 2022: Sensors
https://read.qxmd.com/read/35670599/a-high-throughput-yellow-fever-neutralization-assay
#25
JOURNAL ARTICLE
Madina Rasulova, Thomas Vercruysse, Jasmine Paulissen, Catherina Coun, Vanessa Suin, Leo Heyndrickx, Ji Ma, Katrien Geerts, Jolien Timmermans, Niraj Mishra, Li-Hsin Li, Dieudonné Buh Kum, Lotte Coelmont, Steven Van Gucht, Hadi Karimzadeh, Julia Thorn-Seshold, Simon Rothenfußer, Kevin K Ariën, Johan Neyts, Kai Dallmeier, Hendrik Jan Thibaut
Quick and accurate detection of neutralizing antibodies (nAbs) against yellow fever is essential in serodiagnosis during outbreaks for surveillance and to evaluate vaccine efficacy in population-wide studies. All of this requires serological assays that can process a large number of samples in a highly standardized format. Albeit being laborious, time-consuming, and limited in throughput, the classical plaque reduction neutralization test (PRNT) is still considered the gold standard for the detection and quantification of nAbs due to its sensitivity and specificity...
June 7, 2022: Microbiology Spectrum
https://read.qxmd.com/read/35608626/retrospective-genomic-characterization-of-a-2017-dengue-virus-outbreak-burkina-faso
#26
JOURNAL ARTICLE
Andrew G Letizia, Catherine B Pratt, Michael R Wiley, Anne T Fox, Mba Mosore, Bright Agbodzi, Clara Yeboah, Selassie Kumordjie, Nicholas Di Paola, Kone Cisse Assana, David Coulidiaty, Casimir Ouedraogo, Joseph H Kofi Bonney, William Ampofo, Zékiba Tarnagda, Lassana Sangaré
Knowledge of contemporary genetic composition of dengue virus (DENV) in Africa is lacking. By using next-generation sequencing of samples from the 2017 DENV outbreak in Burkina Faso, we isolated 29 DENV genomes (5 serotype 1, 16 serotype 2 [DENV-2], and 8 serotype 3). Phylogenetic analysis demonstrated the endemic nature of DENV-2 in Burkina Faso. We noted discordant diagnostic results, probably related to genetic divergence between these genomes and the Trioplex PCR. Forward and reverse1 primers had a single mismatch when mapped to the DENV-2 genomes, probably explaining the insensitivity of the molecular test...
June 2022: Emerging Infectious Diseases
https://read.qxmd.com/read/35593182/immunoglobulin-m-seroneutralization-for-improved-confirmation-of-japanese-encephalitis-virus-infection-in-a-flavivirus-endemic-area
#27
JOURNAL ARTICLE
Tehmina Bharucha, Nazli Ayhan, Boris Pastorino, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Anisone Changthongthip, Onanong Sengvilaipaseuth, Ooyanong Phonemixay, Jean-David Pommier, Christopher Gorman, Nicole Zitzmann, Paul N Newton, Xavier de Lamballerie, Audrey Dubot-Pérès
BACKGROUND: The mainstay of diagnostic confirmation of acute Japanese encephalitis (JE) involves detection of anti-JE virus (JEV) immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA). Limitations in the specificity of this test are increasingly apparent with the introduction of JEV vaccinations and the endemicity of other cross-reactive flaviviruses. Virus neutralization testing (VNT) is considered the gold standard, but it is challenging to implement and interpret. We performed a pilot study to assess IgG depletion prior to VNT for detection of anti-JEV IgM neutralizing antibodies (IgM-VNT) as compared with standard VNT...
May 18, 2022: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://read.qxmd.com/read/35487137/combined-detection-of-molecular-and-serological-signatures-of-viral-infections-the-dual-assay-concept
#28
JOURNAL ARTICLE
Débora C Albuquerque, Verónica C Martins, Elisabete Fernandes, Líbia Zé-Zé, Maria João Alves, Susana Cardoso
The recent worldwide spread of viral infections has highlighted the need for accurate, fast, and inexpensive disease diagnosis and monitorization methods. Current diagnostics tend to focus either on molecular or serological testing. In this work we propose a dual detection assay approach for viral diseases, where both serological and molecular assays are combined in a single analysis performed on a magnetoresistive system. This type of assay guarantees an accurate assessment of the infection phase, saving time and costs associated with multiple independent tests...
April 25, 2022: Biosensors & Bioelectronics
https://read.qxmd.com/read/35389998/smartphone-multiplex-microcapillary-diagnostics-using-cygnus-development-and-evaluation-of-rapid-serotype-specific-ns1-detection-with-dengue-patient-samples
#29
JOURNAL ARTICLE
Sarah Helen Needs, Sirintra Sirivisoot, Sophie Jegouic, Tanapan Prommool, Prasit Luangaram, Chatchawan Srisawat, Kanokwan Sriraksa, Wannee Limpitikul, Dumrong Mairiang, Prida Malasit, Panisadee Avirutnan, Chunya Puttikhunt, Alexander Daniel Edwards
Laboratory diagnosis of dengue virus (DENV) infection including DENV serotyping requires skilled labor and well-equipped settings. DENV NS1 lateral flow rapid test (LFT) provides simplicity but lacks ability to identify serotype. A simple, economical, point-of-care device for serotyping is still needed. We present a gravity driven, smartphone compatible, microfluidic device using microcapillary film (MCF) to perform multiplex serotype-specific immunoassay detection of dengue virus NS1. A novel device-termed Cygnus-with a stackable design allows analysis of 1 to 12 samples in parallel in 40 minutes...
April 2022: PLoS Neglected Tropical Diseases
https://read.qxmd.com/read/35309328/development-of-novel-dengue-ns1-multiplex-lateral-flow-immunoassay-to-differentiate-serotypes-in-serum-of-acute-phase-patients-and-infected-mosquitoes
#30
JOURNAL ARTICLE
Szu-Chia Lai, Yu-Yine Huang, Jiunn-Jye Wey, Meng-Hung Tsai, Yi-Ling Chen, Pei-Yun Shu, Shu-Fen Chang, Yi-Jen Hung, Jiu-Nan Hou, Chang-Chi Lin
Dengue is among the most rapidly spreading arboviral disease in the world. A low-cost, easy to use point-of-care diagnostic tool for the detection and differentiation of dengue virus serotypes could improve clinical management, disease prevention, epidemiological surveillance, and outbreak monitoring, particularly in regions where multiple serotypes co-circulate. Despite widespread deployment, no commercial dengue antigen diagnostic test has proven effective in differentiating among dengue virus serotypes. In the current study, we first established mAb pairs and developed a multiplex lateral flow immunoassay for the simultaneous detection of the dengue viral NS1 antigen and identification of serotype...
2022: Frontiers in Immunology
https://read.qxmd.com/read/35108097/mir-573-rescues-endothelial-dysfunction-during-dengue-infection-under-ppar%C3%AE-regulation
#31
JOURNAL ARTICLE
Shefali Banerjee, Chin Wei Xin, Justin Jang Hann Chu
Early prognosis of abnormal vasculopathy is essential for effective clinical management of severe dengue patients. An exaggerated interferon (IFN) response and release of vasoactive factors from endothelial cells cause vasculopathy. This study shows that dengue 2 (DENV2) infection of human umbilical vein endothelial cells (HUVEC) results in differentially regulated miRNAs important for endothelial function. miR-573 was significantly down-regulated in DENV2-infected HUVEC due to decreased Peroxisome Proliferator Activator Receptor Gamma (PPARγ) activity...
February 2, 2022: Journal of Virology
https://read.qxmd.com/read/35091373/differential-impedance-sensing-platform-for-high-selectivity-antibody-detection-down-to-few-counts-a-case-study-on-dengue-virus
#32
JOURNAL ARTICLE
Paola Piedimonte, Laura Sola, Marina Cretich, Alessandro Gori, Marcella Chiari, Edoardo Marchisio, Piero Borga, Riccardo Bertacco, Andrea Melloni, Giorgio Ferrari, Marco Sampietro
We developed a biosensing system for serological detection of viruses based on the impedance variation between gold microelectrodes upon the capture of the target antibodies hybridized with nanobeads for signal amplification. The microfluidic platform core features a Differential Impedance Sensing (DIS) architecture between a reference and an active sensor able to reach nanoparticle resolution of few tens. The biosensor, functionalized with a copoly layer housing a synthetic peptide probe, has shown a limit of detection (LOD) below 100 pg/mL using a model IgG antibody spiked in a buffer...
April 15, 2022: Biosensors & Bioelectronics
https://read.qxmd.com/read/34929668/capacity-of-a-multiplex-igm-antibody-capture-elisa-to-differentiate-zika-and-dengue-virus-infections-in-areas-of-concurrent-endemic-transmission
#33
JOURNAL ARTICLE
Freddy A Medina, Frances Vila, Lakshmanane Premkumar, Olga Lorenzi, Gabriela Paz-Bailey, Luisa I Alvarado, Vanessa Rivera-Amill, Aravinda de Silva, Steve Waterman, Jorge Muñoz-Jordán
Serological cross-reactivity has proved to be a challenge to diagnose Zika virus (ZIKV) infections in dengue virus (DENV) endemic countries. Confirmatory testing of ZIKV IgM positive results by plaque reduction neutralization tests (PRNTs) provides clarification in only a minority of cases because most individuals infected with ZIKV were previously exposed to DENV. The goal of this study was to evaluate the performance of a ZIKV/DENV DUO IgM antibody capture ELISA (MAC-ELISA) for discriminating between DENV and ZIKV infections in endemic regions...
December 20, 2021: American Journal of Tropical Medicine and Hygiene
https://read.qxmd.com/read/34896457/magnetic-multiplex-loop-mediated-isothermal-amplification-mm-lamp-technique-for-simultaneous-detection-of-dengue-and-chikungunya-virus
#34
JOURNAL ARTICLE
Sandeep Kumar, Supriya Sharma, Sarita Kumari, Veena Pande, Deepali Savargaonkar, Anupkumar R Anvikar
Dengue and chikungunya viruses are arthropod borne virus spread through common vector instigating infection in human. There has been an increased recognition that more attention needs to be paid to similar sympotoms caused by both of the virus as they spread in the same region at same time. It warrants need of cost effective, user friendly and rapid multiplex diagnostic technique which could simultaneously diagnose and identify between two virus diseases in resource poor setting. A magnetic multiplex loop mediated isothermal amplification (MM-LAMP) technique was developed by coupling multiplex LAMP with magnetic particle-based naked eye visualization to overcome the shortcoming of simultaneous detection of both diseases...
December 9, 2021: Journal of Virological Methods
https://read.qxmd.com/read/34808091/novel-assay-to-measure-seroprevalence-of-zika-virus-in-the-philippines
#35
JOURNAL ARTICLE
Cameron Adams, Ramesh Jadi, Bruno Segovia-Chumbez, Jedas Daag, Michelle Ylade, Freddy A Medina, Tyler M Sharp, Jorge L Munoz-Jordan, In-Kyu Yoon, Jacqueline Deen, Anna Lena Lopez, Aravinda M de Silva, Lakshmanane Premkumar
Zika virus (ZIKV) is a member of the Flaviviridae family, which includes other clinically notable viruses such as the 4 dengue virus serotypes (DENV-1-4). Distinguishing DENVs from ZIKV using the established serologic assays widely used for monitoring DENV transmission is difficult because of antibody cross-reactivity between these closely related flaviviruses. We describe a modified and improved recombinant envelope domain III-based serologic assay for detecting ZIKV type-specific antibodies in regions with endemic DENV transmission...
December 2021: Emerging Infectious Diseases
https://read.qxmd.com/read/34763153/development-of-a-peptide-aptamer-pair-linked-rapid-fluorescent-diagnostic-system-for-zika-virus-detection
#36
JOURNAL ARTICLE
Anh Thi Viet Nguyen, Bao Tuan Duong, Hyun Park, Seon-Ju Yeo
A rapid diagnostic system employing an antigen detection biosensing method is needed to discriminate between Zika virus (ZIKV) and Dengue virus (DENV) due to their close antigenic homology. We developed a novel peptide pair-based flow immunochromatographic test strip (FICT) assay to detect ZIKV. Peptide aptamers, P6.1 (KQERNNWPLTWT), P29.1 (KYTTSTLKSGV), and B2.33 (KRHVWVSLSYSCAEA) were designed by paratopes and modified against the ZIKV envelope protein based on the binding affinity. An antibody-free lateral FICT was developed using a pair of peptide aptamers...
November 2, 2021: Biosensors & Bioelectronics
https://read.qxmd.com/read/34703255/magnitude-of-antibody-cross-reactivity-in-medically-important-mosquito-borne-flaviviruses-a-systematic-review
#37
REVIEW
Adugna Endale, Girmay Medhin, Koyamo Darfiro, Nigatu Kebede, Mengistu Legesse
INTRODUCTION: Flaviviruses are a genus of enveloped single-stranded RNA viruses that include dengue virus (DENV), yellow fever virus, West Nile virus (WNV), Japanese encephalitis virus, and Zika virus. Nowadays, diverse serological assays are available to diagnose flaviviruses. However, infection with flaviviruses induces cross-reactive antibodies, which are a challenge for serological diagnosis. OBJECTIVE: This systematic review aimed to assess the magnitude of medically important mosquito-borne flavivirus-induced antibody cross-reactivity and its influence on serological test outcomes...
2021: Infection and Drug Resistance
https://read.qxmd.com/read/34699300/improved-radiation-expression-profiling-in-blood-by-sequential-application-of-sensitive-and-specific-gene-signatures
#38
JOURNAL ARTICLE
Eliseos J Mucaki, Ben C Shirley, Peter K Rogan
<u>Purpose</u>. Combinations of expressed genes can discriminate radiation-exposed from normal control blood samples by machine learning based signatures (with 8 to 20% misclassification rates). These signatures can quantify therapeutically-relevant as well as accidental radiation exposures. The prodromal symptoms of Acute Radiation Syndrome (ARS) overlap those present in Influenza and Dengue Fever infections. Surprisingly, these human radiation signatures misclassified gene expression profiles of virally infected samples as false positive exposures...
October 26, 2021: International Journal of Radiation Biology
https://read.qxmd.com/read/34485673/challenges-in-the-control-of-neglected-insect-vector-diseases-of-human-importance-in-the-anglo-caribbean
#39
REVIEW
Sheena Francis, Chelsea Frank, Luke Buchanan, Sean Green, Roxann Stennett-Brown, Georgiana Gordon-Strachan, Yasmin Rubio-Palis, Charles Grant, Ruby Lisa Alexander-Lindo, Chukwuemeka Nwokocha, Dwight Robinson, Rupika Delgoda
Introduction: Neglected tropical diseases (NTDs) in developing countries like the Caribbean, negatively affect multiple income-generating sectors, including the tourism industry upon which island states are highly dependent. Insect-transmitted NTDs include, but are not limited to, malaria, dengue and lymphatic filariasis. Control measures for these disease, are often ignored because of the associated cost. Many of the developing country members are thus retained in a financially crippling cycle, balancing the cost of prophylactic measures with that of controlling an outbreak...
December 2021: One Health
https://read.qxmd.com/read/34372606/laboratory-findings-in-patients-with-probable-dengue-diagnosis-from-an-endemic-area-in-colombia-in-2018
#40
JOURNAL ARTICLE
Jenny C Cardenas, Sandra Y Giraldo-Parra, Maria U Gonzalez, Lady Y Gutierrez-Silva, Lucy Jaimes-Villamizar, Alba L Roa-Parra, Daisy J Carvajal, Hugo O Valdivia, Juan F Sanchez, Tonya M Colpitts, Berlin Londono-Renteria
As demonstrated with the novel coronavirus pandemic, rapid and accurate diagnosis is key to determine the clinical characteristic of a disease and to improve vaccine development. Once the infected person is identified, hematological findings may be used to predict disease outcome and offer the correct treatment. Rapid and accurate diagnosis and clinical parameters are pivotal to track infections during clinical trials and set protection status. This is also applicable for re-emerging diseases like dengue fever, which causes outbreaks in Asia and Latin America every 4 to 5 years...
July 19, 2021: Viruses
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