keyword
MENU ▼
Read by QxMD icon Read
search

Novel and dengue and diagnostic

keyword
https://www.readbyqxmd.com/read/28831630/hands-free-smartphone-based-diagnostics-for-simultaneous-detection-of-zika-chikungunya-and-dengue-at-point-of-care
#1
A Ganguli, A Ornob, H Yu, G L Damhorst, W Chen, F Sun, A Bhuiya, B T Cunningham, R Bashir
Infectious diseases remain the world's top contributors to death and disability, and, with recent outbreaks of Zika virus infections there has been an urgency for simple, sensitive and easily translatable point-of-care tests. Here we demonstrate a novel point-of-care platform to diagnose infectious diseases from whole blood samples. A microfluidic platform performs minimal sample processing in a user-friendly diagnostics card followed by real-time reverse-transcription loop-mediated isothermal amplification (RT-LAMP) on the same card with pre-dried primers specific to viral targets...
August 22, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28768729/evaluation-of-a-novel-reporter-virus-neutralization-test-for-the-serological-diagnosis-of-zika-and-dengue-virus-infection
#2
Chao Shan, Daniel A Ortiz, Yujiao Yang, Susan J Wong, Laura D Kramer, Pei-Yong Shi, Michael J Loeffelholz, Ping Ren
The current laboratory diagnosis of Zika virus (ZIKV) infection is primarily through the detection of ZIKV RNA or antibodies against ZIKV proteins. The detection of viral RNA is highly sensitive and specific, but viremia and viruria periods are brief, limiting the utility of ZIKV RNA assays. Instead, most ZIKV infections are diagnosed serologically using the IgM antibody capture enzyme linked immunosorbent assay (MAC-ELISA) for screening followed by a confirmatory plaque reduction neutralization test (PRNT)...
August 2, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28683141/isolation-of-serotype-specific-antibodies-against-dengue-virus-non-structural-protein-1-using-phage-display-and-application-in-a-multiplexed-serotyping-assay
#3
Kebaneilwe Lebani, Martina L Jones, Daniel Watterson, Andrea Ranzoni, Renee J Traves, Paul R Young, Stephen M Mahler
The multidimensional nature of dengue virus (DENV) infections, which can be caused by four distinct serotypes of the virus, complicates the sensitivity of assays designed for the diagnosis of infection. Different viral markers can be optimally detected at different stages of infection. Of particular clinical importance is the early identification of infection, which is pivotal for disease management and the development of blood screening assays. Non-structural protein 1 (NS1) is an early surrogate marker of infection and its detection in serum coincides with detectable viraemia...
2017: PloS One
https://www.readbyqxmd.com/read/28674411/in-vitro-assembly-and-stabilization-of-dengue-and-zika-virus-envelope-protein-homo-dimers
#4
Stefan W Metz, Emily N Gallichotte, Alex Brackbill, Lakshmanane Premkumar, Michael J Miley, Ralph Baric, Aravinda M de Silva
Zika virus (ZIKV) and the 4 dengue virus (DENV) serotypes are mosquito-borne Flaviviruses that are associated with severe neuronal and hemorrhagic syndromes. The mature flavivirus infectious virion has 90 envelope (E) protein homo-dimers that pack tightly to form a smooth protein coat with icosahedral symmetry. Human antibodies that strongly neutralize ZIKV and DENVs recognize complex quaternary structure epitopes displayed on E-homo-dimers and higher order structures. The ZIKV and DENV E protein expressed as a soluble protein is mainly a monomer that does not display quaternary epitopes, which may explain the modest success with soluble recombinant E (sRecE) as a vaccine and diagnostic antigen...
July 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28604797/a-human-inferred-germline-antibody-binds-to-an-immunodominant-epitope-and-neutralizes-zika-virus
#5
Diogo M Magnani, Cassia G T Silveira, Brandon C Rosen, Michael J Ricciardi, Núria Pedreño-Lopez, Martin J Gutman, Varian K Bailey, Helen S Maxwell, Aline Domingues, Lucas Gonzalez-Nieto, Vivian I Avelino-Silva, Mateus Trindade, Juliana Nogueira, Consuelo S Oliveira, Alvino Maestri, Alvina Clara Felix, José Eduardo Levi, Mauricio L Nogueira, Mauricio A Martins, José M Martinez-Navio, Sebastian P Fuchs, Stephen S Whitehead, Dennis R Burton, Ronald C Desrosiers, Esper G Kallas, David I Watkins
The isolation of neutralizing monoclonal antibodies (nmAbs) against the Zika virus (ZIKV) might lead to novel preventative strategies for infections in at-risk individuals, primarily pregnant women. Here we describe the characterization of human mAbs from the plasmablasts of an acutely infected patient. One of the 18 mAbs had the unusual feature of binding to and neutralizing ZIKV despite not appearing to have been diversified by affinity maturation. This mAb neutralized ZIKV (Neut50 ~ 2 μg/ml) but did not react with any of the four dengue virus serotypes...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28317856/a-smartphone-based-diagnostic-platform-for-rapid-detection-of-zika-chikungunya-and-dengue-viruses
#6
Aashish Priye, Sara W Bird, Yooli K Light, Cameron S Ball, Oscar A Negrete, Robert J Meagher
Current multiplexed diagnostics for Zika, dengue, and chikungunya viruses are situated outside the intersection of affordability, high performance, and suitability for use at the point-of-care in resource-limited settings. Consequently, insufficient diagnostic capabilities are a key limitation facing current Zika outbreak management strategies. Here we demonstrate highly sensitive and specific detection of Zika, chikungunya, and dengue viruses by coupling reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with our recently developed quenching of unincorporated amplification signal reporters (QUASR) technique...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28271980/mosquito-borne-diseases-and-omics-salivary-gland-proteome-of-the-female-aedes-aegypti-mosquito
#7
Rakhi Dhawan, Manish Kumar, Ajeet Kumar Mohanty, Gourav Dey, Jayshree Advani, T S Keshava Prasad, Ashwani Kumar
The female Aedes aegypti mosquito is an important vector for several tropical and subtropical diseases such as dengue, chikungunya, and Zika and yellow fever. The disease viruses infect the mosquito and subsequently spread to the salivary glands after which the viruses can be transmitted to humans with probing or feeding by the mosquito. Omics systems sciences offer the opportunity to characterize vectors and can inform disease surveillance, vector control and development of innovative diagnostics, personalized medicines, vaccines, and insecticide targets...
January 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28185868/dengue-knowledge-gaps-unmet-needs-and-research-priorities
#8
REVIEW
Leah C Katzelnick, Josefina Coloma, Eva Harris
Dengue virus is a mosquito-borne pathogen that causes up to about 100 million cases of disease each year, placing a major public health, social, and economic burden on numerous low-income and middle-income countries. Major advances by investigators, vaccine developers, and affected communities are revealing new insights and enabling novel interventions and approaches to dengue prevention and control. Such research has highlighted further questions about both the basic understanding of dengue and efforts to develop new tools...
March 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28152487/a-heparin-functionalized-carbon-nanotube-based-affinity-biosensor-for-dengue-virus
#9
Daniel Wasik, Ashok Mulchandani, Marylynn V Yates
Dengue virus is an arthropod-borne virus transmitted primarily by Aedes mosquitos and is major cause of disease in tropical and subtropical regions. Colloquially known as Dengue Fever, infection can cause hemorrhagic disorders and death in humans and non-human primates. We report a novel electronic biosensor based on a single-walled carbon nanotube network chemiresistive transducer that is functionalized with heparin for low-cost, label-free, ultra-sensitive, and rapid detection of whole dengue virus (DENV)...
May 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28152075/pathogen-specific-deep-sequence-coupled-biopanning-a-method-for-surveying-human-antibody-responses
#10
Kathryn M Frietze, Juan M Pascale, Brechla Moreno, Bryce Chackerian, David S Peabody
Identifying the targets of antibody responses during infection is important for designing vaccines, developing diagnostic and prognostic tools, and understanding pathogenesis. We developed a novel deep sequence-coupled biopanning approach capable of identifying the protein epitopes of antibodies present in human polyclonal serum. Here, we report the adaptation of this approach for the identification of pathogen-specific epitopes recognized by antibodies elicited during acute infection. As a proof-of-principle, we applied this approach to assessing antibodies to Dengue virus (DENV)...
2017: PloS One
https://www.readbyqxmd.com/read/28006649/serodiagnosis-of-zika-virus-zikv-infections-by-a-novel-ns1-based-elisa-devoid-of-cross-reactivity-with-dengue-virus-antibodies-a-multicohort-study-of-assay-performance-2015-to-2016
#11
Katja Steinhagen, Christian Probst, Christiane Radzimski, Jonas Schmidt-Chanasit, Petra Emmerich, Marjan van Esbroeck, Janke Schinkel, Martin P Grobusch, Abraham Goorhuis, Jens M Warnecke, Erik Lattwein, Lars Komorowski, Andrea Deerberg, Sandra Saschenbrecker, Winfried Stöcker, Wolfgang Schlumberger
Serological diagnosis of Zika virus (ZIKV) infections is challenging due to high cross-reactivity between flaviviruses. We evaluated the diagnostic performance of a novel anti-ZIKV ELISA based on recombinant ZIKV non-structural protein 1 (NS1). Assay sensitivity was examined using sera from 27 patients with reverse transcription (RT)-PCR-confirmed and 85 with suspected ZIKV infection. Specificity was analysed using sera from 1,015 healthy individuals. Samples from 252 patients with dengue virus (n = 93), West Nile virus (n = 34), Japanese encephalitis virus (n = 25), chikungunya virus (n = 19) or Plasmodium spp...
December 15, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/27553703/state-of-the-art-monitoring-in-treatment-of-dengue-shock-syndrome-a-case-series
#12
Steven L Moulton, Jane Mulligan, Anon Srikiatkhachorn, Siripen Kalayanarooj, Greg Z Grudic, Sharone Green, Robert V Gibbons, Gary W Muniz, Carmen Hinojosa-Laborde, Alan L Rothman, Stephen J Thomas, Victor A Convertino
BACKGROUND: Early recognition and treatment of circulatory volume loss is essential in the clinical management of dengue viral infection. We hypothesized that a novel computational algorithm, originally developed for noninvasive monitoring of blood loss in combat casualties, could: (1) indicate the central volume status of children with dengue during the early stages of "shock"; and (2) track fluid resuscitation status. METHODS: Continuous noninvasive photoplethysmographic waveforms were collected over a 5-month period from three children of Thai ethnicity with clinical suspicion of dengue...
August 24, 2016: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/27490379/two-color-lateral-flow-assay-for-multiplex-detection-of-causative-agents-behind-acute-febrile-illnesses
#13
Seoho Lee, Saurabh Mehta, David Erickson
Acute undifferentiated febrile illnesses (AFIs) represent a significant health burden worldwide. AFIs can be caused by infection with a number of different pathogens including dengue (DENV) and Chikungunya viruses (CHIKV), and their differential diagnosis is critical to the proper patient management. While rapid diagnostic tests (RDTs) for the detection of IgG/IgM against a single pathogen have played a significant role in enabling the rapid diagnosis in the point-of-care settings, the state-of-the-art assay scheme is incompatible with the multiplex detection of IgG/IgM to more than one pathogen...
September 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27160350/rapid-low-cost-detection-of-zika-virus-using-programmable-biomolecular-components
#14
Keith Pardee, Alexander A Green, Melissa K Takahashi, Dana Braff, Guillaume Lambert, Jeong Wook Lee, Tom Ferrante, Duo Ma, Nina Donghia, Melina Fan, Nichole M Daringer, Irene Bosch, Dawn M Dudley, David H O'Connor, Lee Gehrke, James J Collins
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences...
May 19, 2016: Cell
https://www.readbyqxmd.com/read/27159058/using-rapid-diagnostic-tests-as-a-source-of-viral-rna-for-dengue-serotyping-by-rt-pcr-a-novel-epidemiological-tool
#15
Manivanh Vongsouvath, Koukeo Phommasone, Onanong Sengvilaipaseuth, Nathamon Kosoltanapiwat, Narisara Chantratita, Stuart D Blacksell, Sue J Lee, Xavier de Lamballerie, Mayfong Mayxay, Sommay Keomany, Paul N Newton, Audrey Dubot-Pérès
BACKGROUND: Dengue virus infection causes major public health problems in tropical and subtropical areas. In many endemic areas, including the Lao PDR, inadequate access to laboratory facilities is a major obstacle to surveillance and study of dengue epidemiology. Filter paper is widely used for blood collection for subsequent laboratory testing for antibody and nucleic acid detection. For the first time, we demonstrate that dengue viral RNA can be extracted from dengue rapid diagnostic tests (RDT) and then submitted to real-time RT-PCR for serotyping...
May 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27126052/high-specificity-of-a-novel-zika-virus-elisa-in-european-patients-after-exposure-to-different-flaviviruses
#16
Daniela Huzly, Ingeborg Hanselmann, Jonas Schmidt-Chanasit, Marcus Panning
The current Zika virus (ZIKV) epidemic in the Americas caused an increase in diagnostic requests in European countries. Here we demonstrate high specificity of the Euroimmun anti-ZIKV IgG and IgM ELISA tests using putative cross-reacting sera of European patients with antibodies against tick-borne encephalitis virus, dengue virus, yellow fever virus and hepatitis C virus. This test may aid in counselling European travellers returning from regions where ZIKV is endemic.
April 21, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/27030492/a-pan-dengue-virus-reverse-transcription-insulated-isothermal-pcr-assay-intended-for-point-of-need-diagnosis-of-dengue-virus-infection-by-use-of-the-pockit-nucleic-acid-analyzer
#17
Yun Young Go, R P V Jayanthe Rajapakse, Senanayake A M Kularatne, Pei-Yu Alison Lee, Keun Bon Ku, Sangwoo Nam, Pin-Hsing Chou, Yun-Long Tsai, Yu-Lun Liu, Hsiao-Fen Grace Chang, Hwa-Tang Thomas Wang, Udeni B R Balasuriya
Dengue virus (DENV) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. Here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal PCR (RT-iiPCR) method for the detection of all four serotypes of DENV in clinical samples. The diagnostic performance of the newly established pan-DENV RT-iiPCR assay targeting a conserved 3' untranslated region of the viral genome was evaluated...
June 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/26729887/defining-acute-kidney-injury-in-dengue-viral-infection-by-conventional-and-novel-classification-systems-akin-and-rifle-a-comparative-analysis
#18
COMPARATIVE STUDY
Tauqeer Hussain Mallhi, Amer Hayat Khan, Azmi Sarriff, Azreen Syazril Adnan, Yusra Habib Khan, Fauziah Jummaat
BACKGROUND: Several criteria have been used to stratify acute kidney injury (AKI) in dengue infection and have resulted in variations in its incidence as well as clinic-laboratory characteristics. The current study was aimed to compare three commonly used criteria of AKI among patients with dengue. METHODS: 667 patients with dengue were defined and staged according to the conventional definition (CD), the Acute Kidney Injury Network (AKIN) and the Risk, Injury, Failure, Loss of function, End stage renal disease (RIFLE) criteria...
February 2016: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/26655854/enhanced-performance-of-an-innovative-dengue-igg-igm-rapid-diagnostic-test-using-an-anti-dengue-edi-monoclonal-antibody-and-dengue-virus-antigen
#19
Jihoo Lee, Young-Eun Kim, Hak-Yong Kim, Mangalam Sinniah, Chom-Kyu Chong, Hyun-Ok Song
High levels of anti-dengue IgM or IgG can be detected using numerous rapid diagnostic tests (RDTs). However, the sensitivity and specificity of these tests are reduced by changes in envelope glycoprotein antigenicity that inevitably occur in limited expression systems. A novel RDT was designed to enhance diagnostic sensitivity. Dengue viruses cultured in animal cells were used as antigens to retain the native viral coat protein. Monoclonal antibodies (mAbs) were then developed, for the first time, against domain I of envelope glycoprotein (EDI)...
December 11, 2015: Scientific Reports
https://www.readbyqxmd.com/read/26536916/quantification-of-ns1-dengue-biomarker-in-serum-via-optomagnetic-nanocluster-detection
#20
Paula Antunes, Daniel Watterson, Mattias Parmvi, Robert Burger, Anja Boisen, Paul Young, Matthew A Cooper, Mikkel F Hansen, Andrea Ranzoni, Marco Donolato
Dengue is a tropical vector-borne disease without cure or vaccine that progressively spreads into regions with temperate climates. Diagnostic tools amenable to resource-limited settings would be highly valuable for epidemiologic control and containment during outbreaks. Here, we present a novel low-cost automated biosensing platform for detection of dengue fever biomarker NS1 and demonstrate it on NS1 spiked in human serum. Magnetic nanoparticles (MNPs) are coated with high-affinity monoclonal antibodies against NS1 via bio-orthogonal Cu-free 'click' chemistry on an anti-fouling surface molecular architecture...
2015: Scientific Reports
keyword
keyword
95873
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"