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Quantity serology and dengue

Sérgio Oliveira De Paula, Cristiane de Melo Lima, Maria Paula Torres, Márcia Rodrigues Garbin Pereira, Benedito Antônio Lopes da Fonseca
Dengue is the most important arboviral disease transmitted to humans. In our laboratory, we have been working on the standardization of the polymerase chain reaction (PCR) diagnosis of this disease. In this work, we compared five commercial kits regularly used on reverse-transcription polymerase chain reaction (RT-PCR) protocols: two Two-Step kits (SuperScript II RT/Super Mix kit and reverse transcription system/Taq DNA polymerase) and three One-Step kits (ready-to-go RT-PCR Beads kit, QIAGEN One-Step RT-PCR kit, and AcessQuick RT-PCR system)...
August 2004: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
P F Vasconcelos, E S Travassos da Rosa, J F Travassos da Rosa, R B de Freitas, N Dégallier, S G Rodrigues, A P Travassos da Rosa
We report the first outbreak of dengue fever caused by dengue 2 (DEN 2) in Araguaina, Tocantins State. Four hundred people of 74 families, living at S. João, Araguaina Sul and Neblina districts were questioned and then bled, in order to obtain sera to test for anti-dengue antibodies. If a person was sick, a small quantity of blood was collected for virus isolation. The main clinical picture of disease was characterized by fever, headache, myalgias, arthralgias and skin rash. Were obtained 1,105 (56 females and 45 males of Culex quinquefasciatus and 567 females and 437 males of Aedes aegypti) mosquitoes from larvae collected in Araguaina...
March 1993: Revista do Instituto de Medicina Tropical de São Paulo
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