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Serology dengue and ns1

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https://www.readbyqxmd.com/read/29411598/a-zika-immunoassay-based-on-surface-enhanced-raman-scattering-sers-nanoprobes
#1
Sabrina Alessio Camacho, Regivaldo Gomes Sobral Filho, Pedro Henrique Benites Aoki, Carlos Jose Leopoldo Constantino, Alexandre G Brolo
Zika virus (ZIKV) was considered a public health emergency of international concern after the 2015 outbreak. Serological tests based on immunoassay platforms is one of the methods applied on the diagnosis of ZIKV and dengue virus (DENV). However, the high limits of detection (LOD) and the cross-reactivity between ZIKV and DENV are still limitations in immunological tests. In order to tackle these issues, we have designed an immune-specific assay based on surface-enhanced Raman scattering (SERS) nanoprobes. Gold shell-isolated nanoparticles (Au-SHINs) were synthesized with 100 nm Au nanoparticles and 4 nm silica shell thickness coated with Nile Blue (Raman reporter)...
February 7, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29409531/development-and-characterization-of-serotype-specific-monoclonal-antibodies-against-the-dengue-virus-4-denv-4-non-structural-protein-ns1
#2
Tesfaye Gelanew, Elizabeth Hunsperger
BACKGROUND: Dengue, caused by one of the four serologically distinct dengue viruses (DENV-1 to - 4), is a mosquito-borne disease of serious global health significance. Reliable and cost-effective diagnostic tests, along with effective vaccines and vector-control strategies, are highly required to reduce dengue morbidity and mortality. Evaluation studies revealed that many commercially available NS1 antigen (Ag) tests have limited sensitivity to DENV-4 serotype compared to the other three serotypes...
February 6, 2018: Virology Journal
https://www.readbyqxmd.com/read/29353669/transmission-of-dengue-virus-from-deceased-donors-to-solid-organ-transplant-recipients-case-report-and-literature-review
#3
Fernando Rosso, Juan C Pineda, Ana M Sanz, Jorge A Cedano, Luis A Caicedo
Dengue fever is a vector-transmitted viral infection. Non-vectorial forms of transmission can occur through organ transplantation. We reviewed medical records of donors and recipients with suspected dengue in the first post-transplant week. We used serologic and molecular analysis to confirm the infection. Herein, we describe four cases of dengue virus transmission through solid organ transplantation. The recipients had positive serology and RT-PCR. Infection in donors was detected through serology. All cases presented with fever within the first week after transplantation...
January 18, 2018: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29305550/comparison-of-four-serological-methods-and-two-rt-pcr-assays-for-diagnosis-and-surveillance-of-zika
#4
Angel Balmaseda, Jose Victor Zambrana, Damaris Collado, Nadezna Garcia, Saira Saborío, Douglas Elizondo, Juan Carlos Mercado, Karla Gonzalez, Cristhiam Cerpas, Andrea Nuñez, Davide Corti, Jesse J Waggoner, Guillermina Kuan, Raquel Burger-Calderon, Eva Harris
Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain Barré Syndrome in adults. Diagnosis and surveillance of Zika in the Americas have been challenging due to similar clinical manifestations and extensive antibody cross-reactivity with endemic flaviviral diseases, like dengue. We evaluated four serological and two RT-PCR methods in acute (mean day 1...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29302646/hospital-and-community-based-surveillance-of-zika-and-dengue-viruses-in-indonesia
#5
Nasronudin Suharto, Muhammad Amin, Diah Puspita Rini, Achmad Chusnu Romdhoni, Purwaningsih Zulfayandi Pawanis, Muhammad Syamsul Hidayat
Background: Zika virus remained silent for few decades after its first recognition in 1947. Recently, Zika drew global attention due to the outbreak in Pacific island and Latin Americas. The concern was escalating given the shared vector of Zika and dengue with possible deleterious complications. Surabaya as one of the largest cities in Indonesia with high mobility of people is endemic to dengue. This study was aimed to detect Zika infection during dengue outbreaks in Surabaya. Materials and Methods: This was a surveillance study involving patients visiting 4 public hospitals and 8 primary health care centres (PHCs)...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29219127/dengue-fever-in-a-municipality-of-west-bengal-india-2015-an-outbreak-investigation
#6
Falguni Debnath, Manickam Ponnaiah, Pralay Acharya
BACKGROUND: In November 2015, death due to fever and increased number of fever cases were reported from Baranagar Municipality of North 24 Parganas district of West Bengal. OBJECTIVES: The episode was investigated with the objective to (1) confirm the existence of an outbreak, (2) describe it in terms of time, place, and person, (3) determine the cause of outbreak, and (4) recommend control measures. METHODS: Monthly incidence of dengue from 2012 to 2014 was calculated and compared with 2015 to confirm the outbreak...
October 2017: Indian Journal of Public Health
https://www.readbyqxmd.com/read/29209371/corrigendum-to-dengue-virus-ns1-protein-as-a-diagnostic-marker-commercially-available-elisa-and-comparison-to-qrt-pcr-and-serological-diagnostic-assays-currently-used-by-the-state-of-florida
#7
Jason H Ambrose, Shamala Devi Sekaran, Azliyati Azizan
[This corrects the article DOI: 10.1155/2017/8072491.].
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29167907/definitive-tests-for-dengue-fever-when-and-which-should-i-use
#8
Hui Bin Yvonne Chan, Choon How How, Chung Wai Mark Ng
Dengue is a mosquito-borne viral disease that has established itself globally in both endemic and epidemic transmission cycles. While diagnostic decision-making for dengue is often guided by clinical judgement, definitive laboratory tests, including rapid point-of-care tests, have many advantages in the primary care setting. These include providing epidemiological data and diagnostic clarity for atypical cases, as well as contributing to patient education and compliance. This article discussed the types of diagnostic methods for dengue, when to use them and the appropriateness of each test...
November 2017: Singapore Medical Journal
https://www.readbyqxmd.com/read/29158907/chikungunya-outbreak-in-delhi-india-2016-report-on-coinfection-status-and-comorbid-conditions-in-patients
#9
N Kaur, J Jain, A Kumar, M Narang, M K Zakaria, A Marcello, D Kumar, R Gaind, S Sunil
Chikungunya fever is a major public health issue in India affecting billions. After 2010, the infection was in a decline until in 2016, when a massive outbreak affected the country. In this report, we present serologic and molecular investigations of 600 patient samples for chikungunya and dengue viruses along with clinical and comorbidity features. We recruited 600 patients during this outbreak and evaluated them for chikungunya and dengue virus antibodies and virus RNA through IgM, NS1 antigen and quantitative real-time PCR (qPCR)...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29107414/kinetics-of-anti-zikv-antibodies-after-zika-infection-using-two-commercial-enzyme-linked-immunoassays
#10
Christophe Pasquier, Guillaume Joguet, Catherine Mengelle, Sabine Chapuy-Regaud, Lynda Pavili, Nadia Prisant, Jacques Izopet, Louis Bujan, Jean-Michel Mansuy
High performance assays are essential for the serological diagnosis of recent and past Zika virus (ZIKV) infections but few are presently available. We used two commercially available NS1 antigen-based enzyme-linked immunoassays to study the kinetics of anti-ZIKV IgM and IgG in 15 ZIKV-infected patients for up to 180days after clinical onset. The Diapro assay detected anti-ZIKV IgM reactivity more frequently (100%) and for longer (median 53days) than did the Euroimmun assay (60%; 13days, P<0.005). Both assays detected anti-ZIKV IgG reactivity 11days after clinical onset in all subjects...
September 15, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29098002/serum-ferritin-a-backstage-weapon-in-diagnosis-of-dengue-fever
#11
Soumyabrata Roy Chaudhuri, Subhayan Bhattacharya, Mainak Chakraborty, Kingshuk Bhattacharjee
Aims: This retrospective study evaluates ferritin as a surrogate marker for dengue infection (NS1 and IgM negative stage) as opposed to other febrile illnesses of infective or inflammatory etiology (OFI). Methodology: Data of all patients admitted to medical ward and medical ITU during the dengue outbreak were collected. Patients admitted between 5 and 10 days of febrile illness without a diagnosis were included. Patients with NS1 positivity (Days 2-8) and/or positive IgM for dengue (Days 6-10) were considered to be dengue cases and those with other confirmed diagnoses were considered in the OFI group...
2017: Interdisciplinary Perspectives on Infectious Diseases
https://www.readbyqxmd.com/read/29020159/distinguishing-secondary-dengue-virus-infection-from-zika-virus-infection-with-previous-dengue-by-a-combination-of-3-simple-serological-tests
#12
Wen-Yang Tsai, Han Ha Youn, Carlos Brites, Jih-Jin Tsai, Jasmine Tyson, Celia Pedroso, Jan Felix Drexler, Mars Stone, Graham Simmons, Michael P Busch, Marion Lanteri, Susan L Stramer, Angel Balmaseda, Eva Harris, Wei-Kung Wang
Background: The explosive spread of Zika virus (ZIKV) and associated microcephaly present an urgent need for sensitive and specific serodiagnostic tests, particularly for pregnant women in dengue virus (DENV)-endemic regions. Recent reports of enhanced ZIKV replication by dengue-immune sera have raised concerns about the role of previous DENV infection on the risk and severity of microcephaly and other ZIKV complications. Methods: Enzyme-linked immunosorbent assays (ELISAs) based on ZIKV and DENV nonstructural protein 1 (NS1) were established to test acute, convalescent phase, and post-convalescent phase serum/plasma samples from reverse-transcription polymerase chain reaction-confirmed cases including 20 primary ZIKV, 25 ZIKV with previous DENV, 58 secondary DENV, and 16 primary DENV1 infections...
November 13, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28954927/rapid-antigen-tests-for-dengue-virus-serotypes-and-zika-virus-in-patient-serum
#13
Irene Bosch, Helena de Puig, Megan Hiley, Marc Carré-Camps, Federico Perdomo-Celis, Carlos F Narváez, Doris M Salgado, Dewahar Senthoor, Madeline O'Grady, Elizabeth Phillips, Ann Durbin, Diana Fandos, Hikaru Miyazaki, Chun-Wan Yen, Margarita Gélvez-Ramírez, Rajas V Warke, Lucas S Ribeiro, Mauro M Teixeira, Roque P Almeida, José E Muñóz-Medina, Juan E Ludert, Mauricio L Nogueira, Tatiana E Colombo, Ana C B Terzian, Patricia T Bozza, Andrea S Calheiros, Yasmine R Vieira, Giselle Barbosa-Lima, Alexandre Vizzoni, José Cerbino-Neto, Fernando A Bozza, Thiago M L Souza, Monique R O Trugilho, Ana M B de Filippis, Patricia C de Sequeira, Ernesto T A Marques, Tereza Magalhaes, Francisco J Díaz, Berta N Restrepo, Katerine Marín, Salim Mattar, Daniel Olson, Edwin J Asturias, Mark Lucera, Mohit Singla, Guruprasad R Medigeshi, Norma de Bosch, Justina Tam, Jose Gómez-Márquez, Charles Clavet, Luis Villar, Kimberly Hamad-Schifferli, Lee Gehrke
The recent Zika virus (ZIKV) outbreak demonstrates that cost-effective clinical diagnostics are urgently needed to detect and distinguish viral infections to improve patient care. Unlike dengue virus (DENV), ZIKV infections during pregnancy correlate with severe birth defects, including microcephaly and neurological disorders. Because ZIKV and DENV are related flaviviruses, their homologous proteins and nucleic acids can cause cross-reactions and false-positive results in molecular, antigenic, and serologic diagnostics...
September 27, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28852878/compensating-for-cross-reactions-using-avidity-and-computation-in-a-suspension-multiplex-immunoassay-for-serotyping-of-zika-versus-other-flavivirus-infections
#14
Bengt Rönnberg, Åke Gustafsson, Olli Vapalahti, Petra Emmerich, Åke Lundkvist, Jonas Schmidt-Chanasit, Jonas Blomberg
The recent spread of Zika virus (ZIKV) in the Americas and Asia necessitates an increased preparedness for improved maternal and perinatal health and blood safety. However, serological cross-reactions, especially to Dengue virus (DENV), complicate ZIKV antibody serodiagnosis. A novel "pan-Flavi" suspension multiplex immunoassay (PFSMIA) using 25 antigens, whole virus (WV), non-structural protein 1 (NS1), and envelope (E) proteins, from 7 zoonotic flaviviruses for specific detection of ZIKV and DENV IgM and IgG was developed...
October 2017: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/28770247/age-of-initial-cohort-of-dengue-patients-could-explain-the-origin-of-disease-outbreak-in-a-setting-a-case-control-study-in-rajasthan-india
#15
Annette Angel, Bennet Angel, Karuna Yadav, Neha Sharma, Vinod Joshi, Indu Thanvi, Sharad Thanvi
Dengue fever (DF) and dengue hemorrhagic fever (DHF) is a public health problem with 390 million cases reported in world annually. In Rajasthan, DF with DHF is being reported for about two decades. For undertaking interventions into disease transmission, locating origin of transmission is very important. Present paper reports retrospective analysis of the hospital reported cases of dengue during the year 2013-2014 undertaken in Barmer, Rajasthan. To address task of investigating outbreak, detailed analysis of the data on serological test results (Mac-ELISA assay of NS1, IgG and IgM) performed by local hospital, Balotra was made...
June 2017: Virusdisease
https://www.readbyqxmd.com/read/28756561/evidence-of-dengue-and-chikungunya-virus-co-infection-and-circulation-of-multiple-dengue-serotypes-in-a-recent-indian-outbreak
#16
S Mukherjee, S K Dutta, S Sengupta, A Tripathi
In India, dengue endemic areas overlap with chikungunya-affected areas and both the viruses are transmitted by same vector, Aedes aegypti - thereby increasing opportunity of co-infection by both viruses. Present study was carried out to understand the DENV-CHIKV infection dynamics during recent outbreaks in eastern India (West Bengal state) and its implication on disease manifestations. Blood was collected from 326 symptomatic febrile patients. Patients' serum was subjected to serological diagnosis for presence of anti-dengue-IgM, anti-chikungunya-IgM antibodies and dengue-NS1 antigen by ELISA...
November 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28740517/dengue-virus-ns1-protein-as-a-diagnostic-marker-commercially-available-elisa-and-comparison-to-qrt-pcr-and-serological-diagnostic-assays-currently-used-by-the-state-of-florida
#17
Jason H Ambrose, Shamala Devi Sekaran, Azliyati Azizan
BACKGROUND: The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both diagnostic approaches. METHODS: This study compared assays currently used for detecting DENV infection at the Florida Department of Health including anti-DENV IgM and IgG ELISAs as well as qRT-PCR, against a commercially available DENV NS1 ELISA...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28726595/co-circulation-and-co-infections-of-all-dengue-virus-serotypes-in-hyderabad-india-2014
#18
K Vaddadi, C Gandikota, P K Jain, V S V Prasad, M Venkataramana
The burden of dengue virus infections increased globally during recent years. Though India is considered as dengue hyper-endemic country, limited data are available on disease epidemiology. The present study includes molecular characterization of dengue virus strains occurred in Hyderabad, India, during the year 2014. A total of 120 febrile cases were recruited for this study, which includes only children and 41 were serologically confirmed for dengue positive infections using non-structural (NS1) and/or IgG/IgM ELISA tests...
September 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28716913/antibody-based-assay-discriminates-zika-virus-infection-from-other-flaviviruses
#19
Angel Balmaseda, Karin Stettler, Raquel Medialdea-Carrera, Damaris Collado, Xia Jin, José Victor Zambrana, Stefano Jaconi, Elisabetta Cameroni, Saira Saborio, Francesca Rovida, Elena Percivalle, Samreen Ijaz, Steve Dicks, Ines Ushiro-Lumb, Luisa Barzon, Patricia Siqueira, David W G Brown, Fausto Baldanti, Richard Tedder, Maria Zambon, A M Bispo de Filippis, Eva Harris, Davide Corti
Zika virus (ZIKV) is a mosquito-borne flavivirus that emerged recently as a global health threat, causing a pandemic in the Americas. ZIKV infection mostly causes mild disease, but is linked to devastating congenital birth defects and Guillain-Barré syndrome in adults. The high level of cross-reactivity among flaviviruses and their cocirculation has complicated serological approaches to differentially detect ZIKV and dengue virus (DENV) infections, accentuating the urgent need for a specific and sensitive serological test...
August 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28706387/comparison-of-nonstructural-protein-1-antigen-detection-by-rapid-and-enzyme-linked-immunosorbent-assay-test-and-its-correlation-with-polymerase-chain-reaction-for-early-diagnosis-of-dengue
#20
Seema Gaikwad, Sandhya S Sawant, Jayanthi S Shastri
INTRODUCTION: Early diagnosis of dengue is important for appropriate clinical management and vector control. Different serological tests based on the principle of immunochromatography and enzyme-linked immunosorbent assay (ELISA) are commonly used for detection of antigen and antibodies of dengue virus. The performance of these tests depends on the sensitivity and specificity. Hence, the study was undertaken to compare nonstructural protein-1 (NS1) antigen detection by rapid and ELISA with real-time polymerase chain reaction (RT-PCR) for diagnosis of dengue...
July 2017: Journal of Laboratory Physicians
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