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Serology dengue and ns1

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https://www.readbyqxmd.com/read/28505571/re-evaluation-of-routine-dengue-virus-serology-in-travelers-in-the-era-of-zika-virus-emergence
#1
Maurits P A van Meer, Ramona Mögling, Janienne Klaasse, Felicity D Chandler, Suzan D Pas, Annemiek A van der Eijk, Marion P G Koopmans, Chantal B E M Reusken, Corine H GeurtsvanKessel
BACKGROUND: Diagnostic requests for both Zika virus (ZIKV) and dengue virus (DENV) infections in returning travelers have significantly increased during the recent ZIKV outbreak in the Americás. These flaviviruses have overlapping clinical syndromes and geographical distribution, but diagnostic differentiation is important because of different clinical consequences. As flaviviruses are known to have a short viremic period, diagnostics often rely on serological methods, which are challenging due to extensive cross-reactive antibodies...
May 3, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28491251/significance-of-igg-optical-density-ratios-index-value-in-single-reactive-anti-dengue-virus-igg-capture-elisa
#2
Shiv Sekhar Chatterjee, Ankush Sharma, Shilpee Choudhury, Sushil Kumar Chumber, Mandeep Kaur, Ras Bage, Nittin Parkhe, Uma Khanduri
BACKGROUND AND OBJECTIVES: A single reactive IgG anti-Dengue virus ELISA test in the absence of IgM antibodies or NS1 antigen may denote current infection or past exposure to the virus. To determine whether IgG index value can be used to identify true current dengue infection we conducted a prospective observational study. MATERIALS AND METHODS: Suspected dengue patients (n =1745) were tested in their first specimen by MAC-ELISA, GAC-ELISA and NS1 antigen ELISA...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28465676/neurological-complications-and-death-in-children-with-dengue-virus-infection-report-of-two-cases
#3
Neydi Osnaya-Romero, Maria-Gabriela Perez-Guille, Sandra Andrade-García, Erika Gonzalez-Vargas, Rebeca Borgaro-Payro, Sandra Villagomez-Martinez, José de Jesús Ortega-Maldonado, Jose Luis Arredondo-García
BACKGROUND: Dengue virus infection can have different complications; the best known is hemorrhagic dengue fever. However, other effects such as neurological disorders may endanger the lives of patients. Dengue neurological manifestations can be confused with encephalitis symptoms and can lead to cerebral edema and death. Therefore, we consider important in the endemic areas to take into account the diagnosis of dengue encephalitis in patients with neurological disorders, and to request the determination of serology in cerebrospinal fluid for the NS1 antigen test...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28254680/enhancing-the-sensitivity-of-dengue-virus-serotype-detection-by-rt-pcr-among-infected-children-in-india
#4
Syed Fazil Ahamed, Rosario Vivek, Shalini Kotabagi, Kaustuv Nayak, Anmol Chandele, Murali-Krishna Kaja, Anita Shet
Dengue surveillance relies on reverse transcription-polymerase chain reaction (RT-PCR), for confirmation of dengue virus (DENV) serotypes. We compared efficacies of published and modified primer sets targeting envelope (Env) and capsid-premembrane (C-prM) genes for detection of circulating DENV serotypes in southern India. Acute samples from children with clinically-diagnosed dengue were used for RT-PCR testing. All samples were also subjected to dengue serology (NS1 antigen and anti-dengue-IgM/IgG rapid immunochromatographic assay)...
February 28, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28094237/a-multiplex-microsphere-immunoassay-for-zika-virus-diagnosis
#5
Susan J Wong, Andrea Furuya, Jing Zou, Xuping Xie, Alan P Dupuis, Laura D Kramer, Pei-Yong Shi
Rapid and accurate diagnosis of infectious agents is essential for patient care, disease control, and countermeasure development. The present serologic diagnosis of Zika virus (ZIKV) infection relies mainly on IgM-capture ELISA which is confounded with the flaw of cross-reactivity among different flaviviruses. In this communication, we report a multiplex microsphere immunoassay (MIA) that captures the diagnostic power of viral envelope protein (that elicits robust, yet cross-reactive antibodies to other flaviviruses) and the differential power of viral nonstructural proteins NS1 and NS5 (that induce more virus-type specific antibodies)...
February 2017: EBioMedicine
https://www.readbyqxmd.com/read/28006649/serodiagnosis-of-zika-virus-zikv-infections-by-a-novel-ns1-based-elisa-devoid-of-cross-reactivity-with-dengue-virus-antibodies-a-multicohort-study-of-assay-performance-2015-to-2016
#6
Katja Steinhagen, Christian Probst, Christiane Radzimski, Jonas Schmidt-Chanasit, Petra Emmerich, Marjan van Esbroeck, Janke Schinkel, Martin P Grobusch, Abraham Goorhuis, Jens M Warnecke, Erik Lattwein, Lars Komorowski, Andrea Deerberg, Sandra Saschenbrecker, Winfried Stöcker, Wolfgang Schlumberger
Serological diagnosis of Zika virus (ZIKV) infections is challenging due to high cross-reactivity between flaviviruses. We evaluated the diagnostic performance of a novel anti-ZIKV ELISA based on recombinant ZIKV non-structural protein 1 (NS1). Assay sensitivity was examined using sera from 27 patients with reverse transcription (RT)-PCR-confirmed and 85 with suspected ZIKV infection. Specificity was analysed using sera from 1,015 healthy individuals. Samples from 252 patients with dengue virus (n = 93), West Nile virus (n = 34), Japanese encephalitis virus (n = 25), chikungunya virus (n = 19) or Plasmodium spp...
December 15, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/27894268/differentiating-secondary-from-primary-dengue-using-igg-to-igm-ratio-in-early-dengue-an-observational-hospital-based-clinico-serological-study-from-north-india
#7
Khalid Hamid Changal, Ab Hameed Raina, Adnan Raina, Manzoor Raina, Rehana Bashir, Muzamil Latief, Tanveer Mir, Qayum Hamid Changal
BACKGROUND: Secondary dengue causes more severe disease than the primary. Early on, it is important to differentiate the two. We tried to find important clinical and laboratory differences between the two for the purpose of early differentiation. METHODS: One hundred fourteen patients confirmed on reverse transcriptase-polymerase chain reaction (RT PCR) were studied. On day 2 of illness IgM and IgG indices were studied for calculation of IgG/IgM ratio. A one-step immunochromatographic assay was used for classification of patients into primary and secondary dengue...
November 28, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27622807/prevalence-and-clinical-course-of-dengue-infection-in-elderly-patients-with-acute-febrile-illness-in-a-tertiary-care-hospital-in-cali-colombia
#8
Fernando Rosso, Sara Vanegas, Sarita Rodríguez, Robinson Pacheco
INTRODUCTION: Little is known about the prevalence and clinical course of dengue infection in elderly patients living in endemic areas; it is presumed that there is a lower prevalence but higher severity, complications and mortality.  OBJECTIVE: To describe the prevalence and clinical course of dengue infection in elderly patients who were admitted to a referral care center for infectious diseases in an endemic region.  MATERIALS AND METHODS: We conducted an observational and descriptive study between 2011 and 2014, using a cohort of elderly patients with serological diagnosis of dengue...
May 3, 2016: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/27621562/clinically-profiling-pediatric-patients-with-dengue
#9
Sriram Pothapregada, Banupriya Kamalakannan, Mahalaskhmy Thulasingham, Srinivasan Sampath
OBJECTIVE: To study the clinical profile and outcome of dengue fever in children at a tertiary care hospital in Puducherry. MATERIALS AND METHODS: All children (0-12 years of age) diagnosed and confirmed as dengue fever from August 2012 to January 2015 were reviewed retrospectively from hospital case records as per the revised World Health Organization guidelines for dengue fever. The diagnosis was confirmed by NS1 antigen-based ELISA test or dengue serology for IgM and IgG antibodies, and the data were analyzed using SPSS 16...
July 2016: Journal of Global Infectious Diseases
https://www.readbyqxmd.com/read/27593529/maintenance-of-demographic-and-hematological-profiles-in-a-long-lasting-dengue-fever-outbreak-implications-for-management
#10
Andréia Moreira Dos Santos Carmo, Rodrigo Buzinaro Suzuki, Michele Marcondes Riquena, André Eterovic, Márcia Aparecida Sperança
BACKGROUND: Dengue fever (DF) outbreaks present regionally specific epidemiological and clinical characteristics. In certain medium-sized cities (100 000-250 000 inhabitants) of São Paulo State, Brazil, and after reaching an incidence of 150 cases/100 000 inhabitants ("epidemiological threshold"), clinical diagnosis indicated dengue virus (DENV) infection. During this period, other seasonally infectious diseases with symptoms and physical signs mimicking DF can simultaneously occur, with the consequential overcrowding of health care facilities as the principal drawbacks...
September 5, 2016: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/27508088/external-quality-assessment-of-dengue-and-chikungunya-diagnostics-in-the-asia-pacific-region-2015
#11
Li Ting Soh, Raynal C Squires, Li Kiang Tan, Kwoon Yong Pok, HuiTing Yang, Christina Liew, Aparna Singh Shah, John Aaskov, Sazaly Abubakar, Futoshi Hasabe, Lee Ching Ng, Frank Konings
OBJECTIVE: To conduct an external quality assessment (EQA) of dengue and chikungunya diagnostics among national-level public health laboratories in the Asia Pacific region following the first round of EQA for dengue diagnostics in 2013. METHODS: Twenty-four national-level public health laboratories performed routine diagnostic assays on a proficiency testing panel consisting of two modules. Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen...
April 2016: Western Pacific Surveillance and Response Journal: WPSAR
https://www.readbyqxmd.com/read/27417494/specificity-cross-reactivity-and-function-of-antibodies-elicited-by-zika-virus-infection
#12
Karin Stettler, Martina Beltramello, Diego A Espinosa, Victoria Graham, Antonino Cassotta, Siro Bianchi, Fabrizia Vanzetta, Andrea Minola, Stefano Jaconi, Federico Mele, Mathilde Foglierini, Mattia Pedotti, Luca Simonelli, Stuart Dowall, Barry Atkinson, Elena Percivalle, Cameron P Simmons, Luca Varani, Johannes Blum, Fausto Baldanti, Elisabetta Cameroni, Roger Hewson, Eva Harris, Antonio Lanzavecchia, Federica Sallusto, Davide Corti
Zika virus (ZIKV), a mosquito-borne flavivirus with homology to Dengue virus (DENV), has become a public health emergency. By characterizing memory lymphocytes from ZIKV-infected patients, we dissected ZIKV-specific and DENV-cross-reactive immune responses. Antibodies to nonstructural protein 1 (NS1) were largely ZIKV-specific and were used to develop a serological diagnostic tool. In contrast, antibodies against E protein domain I/II (EDI/II) were cross-reactive and, although poorly neutralizing, potently enhanced ZIKV and DENV infection in vitro and lethally enhanced DENV disease in mice...
August 19, 2016: Science
https://www.readbyqxmd.com/read/27365962/deadly-intracranial-bleed-in-patients-with-dengue-fever-a-series-of-nine-patients-and-review-of-literature
#13
Jo Ee Sam, Teak Sheng Gee, Abdul Wahab Nasser
INTRODUCTION: Dengue fever is a global pandemic threat with increasing incidence. To date, there are no cures and the effectiveness of dengue vaccines is still uncertain. World Heath Organization introduced expanded dengue syndrome to include unusual presentations of dengue fever including severe neurologic complications. One of the deadly complications is intracranial hemorrhage (ICH). METHODOLOGY: We collected data of patients with ICH diagnosed via a plain computed tomography of the brain (CT brain) with thrombocytopenia and positive Dengue virus type 1 nonstructural protein (NS1) antigen test or positive dengue serology IgM from January 2014 till June 2015 at our center...
July 2016: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/27215933/the-molecular-and-clinical-features-of-dengue-during-outbreak-in-jambi-indonesia-in-2015
#14
Sotianingsih Haryanto, Rahma F Hayati, Benediktus Yohan, Lanceria Sijabat, Ifo F Sihite, Sukmal Fahri, Febrina Meutiawati, Jonathan A N Halim, Stefanie N Halim, Amin Soebandrio, R Tedjo Sasmono
Dengue is hyperendemic in Indonesia. In 2015, reported cases of dengue fever doubled those of 2014 in the Jambi municipality of Sumatra. We examined viral aetiology and its relationship with disease outcome in Jambi. Dengue-suspected patients' sera were collected and NS1 detection and IgM/IgG serology were performed. Dengue virus (DENV) serotyping was performed using real-time RT-PCR. Envelope genes were sequenced to determine the genotypes of DENV. Clinical, haematologic, and demographic data were recorded...
May 2016: Pathogens and Global Health
https://www.readbyqxmd.com/read/27190911/is-reactive-dengue-ns1antigen-test-a-warning-call-for-hospital-admissions
#15
Sriram Pothapregada, Banupriya Kamalakannan, Mahalakshmy Thulasingam, Srinivasan Sampath
INTRODUCTION: Dengue fever is a major public health problem worldwide. The 2011 revised World Health Organization (WHO) guidelines have emphasized on early diagnosis and intervention to reduce the case fatality rate due to dengue fever. Rapid diagnostic tests like NS1 antigen assays have improved the detection of cases in early clinical phase of illness but its role as a predictor of severe dengue infection is not very clear. AIM: To evaluate the utility of NS1 Ag assay as an early diagnostic marker and predictor of severe dengue infection...
April 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27102820/detection-of-dengue-cases-by-serological-testing-in-a-dengue-vaccine-efficacy-trial-utility-for-efficacy-evaluation-and-impact-of-future-vaccine-introduction
#16
Eric Plennevaux, Arunee Sabchareon, Kriengsak Limkittikul, Pornthep Chanthavanich, Chukiat Sirivichayakul, Annick Moureau, Mark Boaz, T Anh Wartel, Melanie Saville, Alain Bouckenooghe
BACKGROUND: Dengue diagnosis confirmation and surveillance are widely based on serological assays to detect anti-dengue IgM or IgG antibodies since such tests are affordable/user-friendly. The World Health Organization identified serological based diagnosis as a potential tool to define probable dengue cases in the context of vaccine trials, while acknowledging that this may have to be interpreted with caution. METHODS: In a phase IIb randomized, placebo-controlled trial assessing the efficacy of a tetravalent dengue vaccine (CYD-TDV) in Thai schoolchildren, case definition was based on virological confirmation by either serotype-specific RT-PCRs or by NS1-antigen ELISA (Clinicaltrials...
May 23, 2016: Vaccine
https://www.readbyqxmd.com/read/26956779/dengue-epidemics-in-two-distinct-periods-reveal-distinct-epidemiological-laboratorial-and-clinical-aspects-in-a-same-scenario-analysis-of-the-2010-and-2013-epidemics-in-mato-grosso-do-sul-brazil
#17
Nieli Rodrigues da Costa Faria, Victor Edgar Fiestas Solorzano, Rita Maria Ribeiro Nogueira, Thaís Chouin-Carneiro, Priscila Conrado Guerra Nunes, Jaqueline Bastos Santos Simões, Fernanda de Bruycker Nogueira, Monique da Rocha Queiroz Lima, Luzia Maria de Oliveira Pinto, Claire Fernandes Kubelka, Rivaldo Venâncio da Cunha, Elzinandes Leal de Azeredo, Flavia Barreto dos Santos
BACKGROUND: Dengue is a major problem in Brazil. Epidemiological and clinical aspects were characterized in patients from two epidemics which occurred in Mato Grosso do Sul, Brazil. METHODS: Dengue cases were classified according to the 2009 WHO criteria, tested by serological and molecular biology tests and analysed for nonstructural protein 1 (NS1) antigenemia. RESULTS: Dengue was confirmed in 78.7% (48/61) and 75.6% (118/156) of the cases studied in 2010 and 2013, respectively...
April 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/26846603/is-ultrasound-a-useful-tool-to-predict-severe-dengue-infection
#18
Sriram Pothapregada, Poonam Kullu, Banupriya Kamalakannan, Mahalakshmy Thulasingam
OBJECTIVES: To study the role of ultrasound in children with dengue fever and determine its role in predicting the severity of the disease. METHODS: This was a retrospective hospital based study conducted from 1(st) August 2012 to January 31(st) 2015 at a tertiary care hospital in Puducherry. RESULTS: Two hundred and fifty four children were admitted with dengue fever and among them non-severe dengue and severe dengue were seen in 62.6 % and 37...
June 2016: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/26844445/serological-evidence-of-leptospirosis-in-patients-with-a-clinical-suspicion-of-dengue-in-the-state-of-cear%C3%A3-brazil
#19
Laiane Fernanda de Melo Bezerra, Raissa Matos Fontes, Almira Maria Monteiro Gomes, Dyana Alves da Silva, Jeová Keny Baima Colares, Danielle Malta Lima
INTRODUCTION: Dengue and leptospirosis are two febrile illnesses of great clinical and epidemiological importance in Brazil. Their significant degree of symptomatic similarity makes clinical diagnosis difficult. OBJECTIVE: To diagnose leptospirosis differentially in patients with clinically suspected dengue. MATERIALS AND METHODS: In this study, 86 patients with clinically suspected dengue underwent virological and serological diagnostic evaluations for dengue (reverse transcriptase polymerase chain reaction, NS1 immunochromatographic test, and NS1 enzyme-linked immunosorbent assay, ELISA), as well as tests to detect immunoglobulin M (IgM; IgM/IgG Rapid Test and IgM ELISA)...
October 2015: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/26776124/a-study-on-gender-related-differences-in-laboratory-characteristics-of-dengue-fever
#20
A Chakravarti, P Roy, S Malik, O Siddiqui, P Thakur
Studies have reported significant gender-related differences in serological tests for detection of NS1 antigen and IgM antibody used for diagnosing dengue fever. However, no such study has been undertaken in India though dengue fever is endemic in this country. Therefore, this study was planned to study the association of serological findings with gender in 700 patients suspected to be suffering from dengue fever in the Indian setting. Haematological parameters of seropositive patients were also studied. Seropositivity and haemorrhagic findings were significantly associated with the female gender...
January 2016: Indian Journal of Medical Microbiology
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