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https://www.readbyqxmd.com/read/28938792/gastrointestinal-microbial-population-of-turkey-meleagris-gallopavo-affected-by-hemorrhagic-enteritis-virus
#1
Sara D'Andreano, Armand Sànchez Bonastre, Olga Francino, Anna Cuscó Martí, Cristina Lecchi, Guido Grilli, Davide Giovanardi, Fabrizio Ceciliani
Hemorrhagic enteritis (HE) is an acute viral disease that affects avian species, particularly turkeys, compromising their commercial production and having a negative effect on animal welfare. Turkey adenovirus 3 (TAdV-3), is the main causal agent of the disease. In this study, we considered 3 groups of turkeys to achieve 2 purposes: 1) A preliminary investigation on the microbiota content in the 4 parts of healthy turkey's intestine (group A), namely duodenum, jejunum, ileum, and ceca was done; 2) an investigation on the relationship between natural infections with TAdV-3 and the intestinal microbiota in the jejunum, where HE mostly develops, comparing group A with animals with molecular positivity for the virus and with clinical signs of HE (group B) and animals with molecular positivity for the virus but without clinical signs (group C)...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28938780/effects-of-dietary-threonine-supplementation-on-intestinal-barrier-function-and-gut-microbiota-of-laying-hens
#2
X Y Dong, M M M Azzam, X T Zou
The effects of supplemental dietary threonine (Thr) on laying performance, expression of intestinal mucin 2 (MUC2) and secretory IgA (sIgA), and intestinal microbiota of laying hens fed a low CP diet were investigated. A total of 240 Lohmann Brown laying hens, 28 wk of age, was allocated to 3 dietary treatments, each of which included 5 replicates of 16 hens. Hens were fed a control diet (16% CP), a low CP diet (14% CP), or a low CP diet supplemented with 0.3% L-Thr for 12 weeks. Chemical analyses of the diets for Thr are 0...
October 1, 2017: Poultry Science
https://www.readbyqxmd.com/read/28938676/risk-factors-for-mortality-from-pneumocystis-carinii-pneumonia-pcp-in-non-hiv-patients-a-meta-analysis
#3
Yao Liu, Lili Su, Shu-Juan Jiang, Hui Qu
The number of patients with non-human immunodeficiency virus (HIV) related pneumocystis carinii pneumonia (PCP) is increasing with widespread immunosuppressive treatment. We performed a meta-analysis to describe the clinical characteristics and factors associated with outcomes of PCP in HIV-negative patients. A total of 13 studies including 867 patients with non-HIV related PCP was included. The overall mortality for non-HIV patients with PCP was 30.6%. The most common underlying disorder for the development of PCP is hematological malignancies (29...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938380/bacterial-keratitis-in-toronto-a-16-year-review-of-the-microorganisms-isolated-and-the-resistance-patterns-observed
#4
Alex L C Tam, Elie Côté, Mario Saldanha, Alejandro Lichtinger, Allan R Slomovic
PURPOSE: To review the incidence, distribution, current trends, and resistance patterns of bacterial keratitis isolates in Toronto over the past 16 years. METHODS: Microbiology records of suspected bacterial keratitis that underwent a diagnostic corneal scraping and cultures from January 1, 2000, through December 31, 2015, were retrospectively reviewed. The distribution of the main isolated pathogens and in vitro laboratory minimum inhibitory concentration testing results were used to identify resistance patterns...
September 21, 2017: Cornea
https://www.readbyqxmd.com/read/28938209/interactions-between-biogenic-selenium-nanoparticles-and-goethite-colloids-and-consequence-for-remediation-of-elemental-mercury-contaminated-groundwater
#5
Xiaonan Wang, Daoyong Zhang, Haifeng Qian, Yan Liang, Xiangliang Pan, Geoffrey Michael Gadd
Ubiquitous colloidal minerals such as goethite can have a significant impact on the performance of nanoparticles-based groundwater remediation due to aggregation. Heteroaggregation and retention of Se nanoparticles (SeNPs) by goethite in groundwater, and its impact on Hg(0) remediation by SeNPs were investigated in this study. In order to mitigate the adverse effects of aggregation, the effects of bacterial extracellular polymeric substances (EPS) on the stability of SeNPs and Hg(0) sequestration using SeNPs were also evaluated...
September 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28938203/green-synthesis-biological-and-spectroscopic-study-on-the-interaction-of-multi-component-mannich-bases-of-imidazo-2-1-b-benzothiazoles-with-human-serum-albumin
#6
Swetha K Maddili, Leela Prasad Yandrati, Shakuntala Siddam, Vijaya Kumar Kannekanti, Himabindu Gandham
A series of Mannich bases of imidazo[2, l-b]benzothiazoles were prepared through one-pot multi-component reaction in the presence of water as an eco-friendly solvent. All the synthesized compounds were confirmed from IR, (1)HNMR, (13)CNMR, and Mass spectroscopy. Evaluation of in vitro anti-inflammatory and anti-microbial activities of all the synthesized derivatives was further accomplished. These results clearly displayed that compound 6d exhibited outstanding anti-inflammatory activity with a percentage inhibition of 70...
August 19, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28938202/effect-of-different-diode-laser-wavelengths-on-root-dentin-decontamination-infected-with-enterococcus-faecalis
#7
Caroline Cristina Borges, Carlos Estrela, Fabiane Carneiro Lopes, Regina Guenka Palma-Dibb, Jesus Djalma Pecora, Cyntia Rodrigues De Araújo Estrela, Manoel Damião de Sousa-Neto
The objective of this study was to evaluate the antibacterial effect and the ultrastructural alterations of diode laser with different wavelengths (808nm and 970nm) and its association with irrigating solutions (2.5% sodium hypochlorite and 2% chlorhexidine) in root dentin contaminated by a five days biofilm. Thirteen uniradicular teeth were sectioned into 100 dentin intraradicular blocks. Initially, the blocks were immersed for 5min in 17% EDTA and washed with distilled water for 5min, then samples were sterilized for 30min at 120°C...
September 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28938097/effect-of-sos-induced-levels-of-imuabc-on-spontaneous-and-damage-induced-mutagenesis-in-caulobacter-crescentus
#8
Ingrid R Alves, Marco A Lima-Noronha, Larissa G Silva, Frank S Fernández-Silva, Aline Luiza D Freitas, Marilis V Marques, Rodrigo S Galhardo
imuABC (imuAB dnaE2) genes are responsible for SOS-mutagenesis in Caulobacter crescentus and other bacterial species devoid of umuDC. In this work, we have constructed operator-constitutive mutants of the imuABC operon. We used this genetic tool to investigate the effect of SOS-induced levels of these genes upon both spontaneous and damage-induced mutagenesis. We showed that constitutive expression of imuABC does not increase spontaneous or damage-induced mutagenesis, nor increases cellular resistance to DNA-damaging agents...
September 14, 2017: DNA Repair
https://www.readbyqxmd.com/read/28938028/association-between-implant-abutment-microgap-and-implant-circularity-to-bacterial-leakage-an-in-vitro-study-using-tapered-connection-implants
#9
Eduardo Cláudio Lopes de Chaves E Mello Dias, Marcelo Sperandio, Marcelo Henrique Napimoga
PURPOSE: The aim of this study was to evaluate the microgap between the abutment and implant as well as the circularity of implant platforms and associating conformational errors with bacterial microleakage in tapered connection implant systems. MATERIALS AND METHODS: Four brands of implants with a tapered abutment connection were tested. Bacterial leakage was assessed using 0.3 μL of Escherichia coli suspension inoculated into the abutment screw chamber of the implants, which were then torqued and incubated at 37°C for 14 days...
September 22, 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28938018/genome-wide-identification-of-bacterial-plant-colonization-genes
#10
Benjamin J Cole, Meghan E Feltcher, Robert J Waters, Kelly M Wetmore, Tatiana S Mucyn, Elizabeth M Ryan, Gaoyan Wang, Sabah Ul-Hasan, Meredith McDonald, Yasuo Yoshikuni, Rex R Malmstrom, Adam M Deutschbauer, Jeffery L Dangl, Axel Visel
Diverse soil-resident bacteria can contribute to plant growth and health, but the molecular mechanisms enabling them to effectively colonize their plant hosts remain poorly understood. We used randomly barcoded transposon mutagenesis sequencing (RB-TnSeq) in Pseudomonas simiae, a model root-colonizing bacterium, to establish a genome-wide map of bacterial genes required for colonization of the Arabidopsis thaliana root system. We identified 115 genes (2% of all P. simiae genes) with functions that are required for maximal competitive colonization of the root system...
September 22, 2017: PLoS Biology
https://www.readbyqxmd.com/read/28937897/the-role-of-genetic-factors-in-the-outbreak-mechanism-of-dental-caries
#11
Junko Shimomura-Kuroki, Tomoko Nashida, Yukio Miyagawa, Tsuneo Sekimoto
OBJECTIVE: The aim of the present study was to investigate the relationships between cariogenic bacterial infection and single nucleotide polymorphisms (SNPs) in candidate genes associated with dental caries, and to explore the factors related to caries in children. STUDY DESIGN: Children aged 3 to 11 years were selected. Detection of cariogenic bacteria (Streptococcus mutans, Streptococcus oralis, Streptococcus sobrinus and Lactobacillus) from the plaque of each patient, and SNP analyses of five candidate genes (MBL2, TAS2R38, GLUT2, MMP13 and CA6) were performed using DNA isolated from buccal mucosal cells...
September 22, 2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28937894/microbiologic-evaluation-of-cotton-and-polytetrafluoroethylene-ptfe-tape-as-endodontic-spacer-materials-in-primary-molars-an-in-vivo-study
#12
Attiguppe Ramasetty Prabhakar, Kratika Dixit, O S Raju
OBJECTIVE: PTFE tape, which is commonly used as plumber's tape is an inorganic, non-fibrous, ribbon like material. The aim of this study was to evaluate PTFE tape as endodontic spacer material and to compare it with commonly used spacer material that is cotton, in primary teeth. STUDY DESIGN: Seventeen children undergoing pulpectomy of lower second primary molar bilaterally were included in the study. Cotton and PTFE tape were placed as spacers on each side randomly...
September 22, 2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28937879/advax4-delta-inulin-combination-adjuvant-together-with-ecmx-a-fusion-construct-of-three-protective-mtb-antigens-induces-a-potent-th1-immune-response-and-protects-mice-against-mycobacterium-tuberculosis-infection
#13
Bruno de Paula Oliveira Santos, Monalisa Martins Trentini, Renato Beilner Machado, Mara Rúbia Nunes Celes, André Kipnis, Nikolai Petrovsky, Ana Paula Junqueira-Kipnis
Tuberculosis (TB) remains a main public health concern and 10.4 million new cases occurred in 2015 around the world. BCG is the only approved vaccine against TB, but has variable efficacy and new vaccines are needed. We developed two new mTB vaccine candidates based on the recombinant fusion proteins, rCMX and rECMXformulated with Advax4, a new combination adjuvant combining delta inulin, CpG oligonucleotide and murabutide. When BALB/c mice were immunized three times intramuscularly with these vaccine formulationsInjection of Advax4 alone increased the percentage of lymphatic endothelial cells and activated macrophages (F480/CD11b(+)) in the draining lymph nodes consistent with a chemotactic adjuvant effect...
September 22, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28937878/degradation-of-chlorpyrifos-by-an-endophytic-bacterium-of-the-sphingomonas-genus-strain-hjy-isolated-from-chinese-chives-allium-tuberosum
#14
Fayun Feng, Yisong Li, Jing Ge, Jinjin Chen, Wayne Jiang, Shuang He, Xianjing Liu, Xiangyang Yu
The degradation of chlorpyrifos (CP) by an endophytic bacterial strain (HJY) isolated from Chinese chives (Allium tuberosum Rottl. ex Spreng) was investigated. Strain HJY was identified as Sphingomonas sp. based on morphological, physiological, and biochemical tests and a 16S rDNA sequence analysis. Approximately 96% of 20 mg L(-1) CP was degraded by strain HJY over 15 days in liquid minimal salts medium (MSM). The CP degradation rate could also be increased by glucose supplementation. The optimal conditions for the removal of 20 mg L(-1) CP by strain HJY in MSM were 2% inoculum density, pH 6...
September 22, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28937735/whole-genome-shotgun-sequencing-and-comparison-of-two-%C3%AE-proteobacterial-species-in-search-of-the-bulgecin-biosynthetic-cluster
#15
Mark E Horsman, Daniel R Marous, Rongfeng Li, Ryan A Oliver, Byungjin Byun, Scott J Emrich, Bill Boggess, Craig A Townsend, Shahriar Mobashery
We have produced draft whole-genome sequences for two bacterial strains reported to produce the bulgecins as well as NRPS-derived monobactam β-lactam antibiotics. We propose reclassification of ATCC 31363 from the original Pseudomonas genus to Paraburkholderia acidophila. We further reaffirm that ATCC 31433 (Burkholderia mesoacidophila) is a taxonomically distinct producer of bulgecins with notable gene regions shared with Paraburkholderia acidophila. We use RAST multiple gene comparison and MASH distancing with published genomes to order the draft contigs and identify unique gene regions for characterization...
September 22, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28937621/the-development-of-a-melt-extruded-shellac-carrier-for-the-targeted-delivery-of-probiotics-to-the-colon
#16
Noel M Gately, James E Kennedy
Hot melt extrusion (HME) is considered an efficient technique in developing solid molecular dispersions, and has been demonstrated to provide sustained, modified and targeted drug delivery resulting in improved bioavailability. However, most commercial enteric or pH-responsive polymers are relatively difficult to process or have high Glass Transition Temperature (Tg) values, making their use with temperature-sensitive drugs, probiotics or biologics not viable. Shellac is a natural thermoplastic, and after a review of current literature on the pharmaceutical HME process, a possible gap in the knowledge of the use of shellac to produce dosage forms by means of HME was identified...
September 22, 2017: Pharmaceutics
https://www.readbyqxmd.com/read/28937612/houttuynia-cordata-facilitates-metformin-on-ameliorating-insulin-resistance-associated-with-gut-microbiota-alteration-in-oletf-rats
#17
Jing-Hua Wang, Shambhunath Bose, Soo-Kyoung Lim, AbuZar Ansari, Young-Won Chin, Han Seok Choi, Hojun Kim
Metformin and Houttuynia cordata are representative anti-diabetic therapeutics in western and oriental medicine, respectively. The current study examined the synergistic anti-diabetic effect of Houttuynia cordata extraction (HCE) and metformin combination in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Fecal microbiota were analyzed by denaturing gradient gel electrophoresis (DGGE) and real-time PCR. Combining HCE + metformin resulted in significantly ameliorated glucose tolerance (oral glucose tolerance test (OGTT))-the same as metformin alone...
September 22, 2017: Genes
https://www.readbyqxmd.com/read/28937414/rheumatoid-arthritis-and-citrullination
#18
Erika Darrah, Felipe Andrade
PURPOSE OF REVIEW: Dysregulated citrullination is a key element that drives the production and maintenance of antibodies to citrullinated proteins, a hallmark in rheumatoid arthritis (RA). This article reviews recent literature on the origin of citrullinated antigens in RA. RECENT FINDINGS: The study of synovial fluid from patients with RA has provided important insights into the identity of citrullinated proteins that accumulate in the RA joint (the RA citrullinome) and mechanisms that control their generation...
September 21, 2017: Current Opinion in Rheumatology
https://www.readbyqxmd.com/read/28937234/experimental-models-of-acute-lung-injury-in-the-newborns
#19
D Mokra, A Calkovska
Acute lung injury in the preterm newborns can originate from prematurity of the lung and insufficient synthesis of pulmonary surfactant. This situation is known as respiratory distress syndrome (RDS). In the term neonates, the respiratory insufficiency is related to a secondary inactivation of the pulmonary surfactant, for instance, by action of endotoxins in bacterial pneumonia or by effects of aspirated meconium. The use of experimental models of the mentioned situations provides new information on the pathophysiology of these disorders and offers unique possibility to test novel therapeutic approaches in the conditions which are very similar to the clinical syndromes...
September 22, 2017: Physiological Research
https://www.readbyqxmd.com/read/28937231/pulmonary-surfactant-and-bacterial-lipopolysaccharide-the-interaction-and-its-functional-consequences
#20
M Kolomaznik, Z Nova, A Calkovska
The respiratory system is constantly exposed to pathogens which enter the lungs by inhalation or via blood stream. Lipopolysaccharide (LPS), also named endotoxin, can reach the airspaces as the major component of the outer membrane of Gram-negative bacteria, and lead to local inflammation and systemic toxicity. LPS affects alveolar type II (ATII) cells and pulmonary surfactant and although surfactant molecule has the effective protective mechanisms, excessive amount of LPS interacts with surfactant film and leads to its inactivation...
September 22, 2017: Physiological Research
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