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https://www.readbyqxmd.com/read/29145645/waddlia-chondrophila-a-chlamydia-related-bacterium-has-a-negative-impact-on-human-spermatozoa
#1
D Baud, N Vulliemoz, A Ammerdorffer, J Gyger, G Greub, V Castella, M Stojanov
STUDY QUESTION: What is the impact of Waddlia chondrophila, an emerging Chlamydia-related bacterium associated with miscarriage, on human spermatozoa? SUMMARY ANSWER: W. chondrophila had a negative impact on human spermatozoa (decrease in viability and mitochondrial membrane potential) and was not entirely removed from infected samples by density gradient centrifugation. WHAT IS KNOWN ALREADY: Bacterial infection or colonization might have a deleterious effect on male fertility...
November 14, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29145643/the-merops-database-of-proteolytic-enzymes-their-substrates-and-inhibitors-in-2017-and-a-comparison-with-peptidases-in-the-panther-database
#2
Neil D Rawlings, Alan J Barrett, Paul D Thomas, Xiaosong Huang, Alex Bateman, Robert D Finn
The MEROPS database (http://www.ebi.ac.uk/merops/) is an integrated source of information about peptidases, their substrates and inhibitors. The hierarchical classification is: protein-species, family, clan, with an identifier at each level. The MEROPS website moved to the EMBL-EBI in 2017, requiring refactoring of the code-base and services provided. The interface to sequence searching has changed and the MEROPS protein sequence libraries can be searched at the EMBL-EBI with HMMER, FastA and BLASTP. Cross-references have been established between MEROPS and the PANTHER database at both the family and protein-species level, which will help to improve curation and coverage between the resources...
November 14, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29145635/trex-a-web-portal-for-exploration-of-trna-derived-fragments-in-arabidopsis-thaliana
#3
Agnieszka Thompson, Andrzej Zielezinski, Patrycja Plewka, Maciej Szymanski, Przemyslaw Nuc, Zofia Szweykowska-Kulinska, Artur Jarmolowski, Wojciech M Karlowski
tRNA-derived fragments (tRFs) constitute a new class of short regulatory RNAs that are a product of nascent or mature tRNA processing. tRF sequences have been identified in all domains of life; however, most published research pertains to human, yeast and some bacterial organisms. Despite growing interest in plant tRFs and accumulating evidence of their function in plant development and stress responses, no public, web-based repository dedicated to these molecules is currently available. Here, we introduce tRex (http://combio...
November 14, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29145627/bacterial-communities-associated-with-the-ectoparasitic-mites-varroa-destructor-and-tropilaelaps-mercedesae-of-honey-bees-apis-mellifera
#4
Chonthicha Pakwan, Martin Kaltenpoth, Benjamin Weiss, Panuwan Chantawannakul, Guo Jun, Terd Disayathanoowat
Varroa and Tropilaelaps mites have been reported as serious ectoparasites of honey bees (Apis mellifera). In this study, bacterial communities associated with Varroa destructor and Tropilaelaps mercedesae from Northern Thailand were determined, using both culture-dependent and culture-independent approaches. Adult female mites were collected from apiaries in Chiang Mai and Lampang provinces. Culturable bacteria were isolated from individual mites. On average, we observed approximately 1340 and 1140 CFU/mite in Varroa and Tropilaelaps, respectively...
November 14, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29145612/developmental-and-gut-related-changes-to-microbiomes-of-the-cultured-juvenile-spiny-lobster-panulirus-ornatus
#5
Mei C Ooi, Evan F Goulden, Gregory G Smith, Barbara F Nowak, Andrew R Bridle
With recent technologies making it possible for commercial scale closed life-cycle aquaculture production of spiny lobster (Panulirus ornatus) comes a strong impetus to further understand aspects of lobster health. The gut microbiome plays a crucial role in host health, affecting growth, digestion, immune responses and pathogen resistance. Herein we characterise and compare gut microbiomes across different developmental stages (6-7 d post-emergence [dpe], 52 dpe and 13 months post-emergence [mpe]) and gut regions (foregut, midgut and hindgut) of cultured P...
November 14, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29145592/soil-bacterial-community-response-to-altered-precipitation-and-temperature-regimes-in-an-old-field-grassland-are-mediated-by-plants
#6
Akihiro Koyama, J Megan Steinweg, Michelle L Haddix, Jeffrey S Dukes, Matthew D Wallenstein
The structure and function of soil microbiomes often change in response to experimental climate manipulations, suggesting an important role in ecosystem feedbacks. However, it is difficult to know if microbes are responding directly to environmental changes or are more strongly impacted by plant responses. We investigated soil microbial responses to precipitation and temperature manipulations at the Boston-Area Climate Experiment in Massachusetts, USA in both vegetated and bare plots to parse direct vs plant-mediated response to multi-factor climate change...
November 14, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29145477/the-abundance-of-health-associated-bacteria-is-altered-in-pah-polluted-soils-implications-for-health-in-urban-areas
#7
Anirudra Parajuli, Mira Grönroos, Sari Kauppi, Tomasz Płociniczak, Marja I Roslund, Polina Galitskaya, Olli H Laitinen, Heikki Hyöty, Ari Jumpponen, Rauni Strömmer, Martin Romantschuk, Nan Hui, Aki Sinkkonen
Long-term exposure to polyaromatic hydrocarbons (PAHs) has been connected to chronic human health disorders. It is also well-known that i) PAH contamination alters soil bacterial communities, ii) human microbiome is associated with environmental microbiome, and iii) alteration in the abundance of members in several bacterial phyla is associated with adverse or beneficial human health effects. We hypothesized that soil pollution by PAHs altered soil bacterial communities that had known associations with human health...
2017: PloS One
https://www.readbyqxmd.com/read/29145425/statistical-analysis-of-co-occurrence-patterns-in-microbial-presence-absence-datasets
#8
Kumar P Mainali, Sharon Bewick, Peter Thielen, Thomas Mehoke, Florian P Breitwieser, Shishir Paudel, Arjun Adhikari, Joshua Wolfe, Eric V Slud, David Karig, William F Fagan
Drawing on a long history in macroecology, correlation analysis of microbiome datasets is becoming a common practice for identifying relationships or shared ecological niches among bacterial taxa. However, many of the statistical issues that plague such analyses in macroscale communities remain unresolved for microbial communities. Here, we discuss problems in the analysis of microbial species correlations based on presence-absence data. We focus on presence-absence data because this information is more readily obtainable from sequencing studies, especially for whole-genome sequencing, where abundance estimation is still in its infancy...
2017: PloS One
https://www.readbyqxmd.com/read/29145396/detection-of-relapsing-fever-borrelia-spp-bartonella-spp-and-anaplasmataceae-bacteria-in-argasid-ticks-in-algeria
#9
Ismail Lafri, Basma El Hamzaoui, Idir Bitam, Hamza Leulmi, Reda Lalout, Oleg Mediannikov, Mohamed Chergui, Mohamed Karakellah, Didier Raoult, Philippe Parola
BACKGROUND: Argasid ticks (soft ticks) are blood-feeding arthropods that can parasitize rodents, birds, humans, livestock and companion animals. Ticks of the Ornithodoros genus are known to be vectors of relapsing fever borreliosis in humans. In Algeria, little is known about relapsing fever borreliosis and other bacterial pathogens transmitted by argasid ticks. METHODOLOGY/PRINCIPAL FINDINGS: Between May 2013 and October 2015, we investigated the presence of soft ticks in 20 rodent burrows, 10 yellow-legged gull (Larus michahellis) nests and animal shelters in six locations in two different bioclimatic zones in Algeria...
November 16, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29145277/tricho-hepato-enteric-syndrome-with-novel-skiv2l-gene-mutations-a-case-report
#10
Eitaro Hiejima, Takahiro Yasumi, Hiroshi Nakase, Minoru Matsuura, Yusuke Honzawa, Hirokazu Higuchi, Ikuo Okafuji, Tohru Yorifuji, Takayuki Tanaka, Kazushi Izawa, Tomoki Kawai, Ryuta Nishikomori, Toshio Heike
RATIONALE: Tricho-hepato-enteric syndrome (THES) is a rare disorder caused by mutations in the TTC37 or SKIV2L genes and characterized by chronic diarrhea, liver disease, hair abnormalities, and high mortality in early childhood due to severe infection or liver cirrhosis. PATIENT CONCERNS: The patient is the second child of three siblings born to non-consanguineous healthy Japanese parents. She had intrauterine growth retardation and was delivered at 33 weeks of gestation due to placental abruption...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29145176/the-membrane-attack-complex-perforin-superfamily
#11
Gabriel Moreno-Hagelsieb, Bennett Vitug, Arturo Medrano-Soto, Milton H Saier
The membrane attack complex/perforin (MACPF) superfamily consists of a diverse group of proteins involved in bacterial pathogenesis and sporulation as well as eukaryotic immunity, embryonic development, neural migration and fruiting body formation. The present work shows that the evolutionary relationships between the members of the superfamily, previously suggested by comparison of their tertiary structures, can also be supported by analyses of their primary structures. The superfamily includes the MACPF family (TC 1...
November 17, 2017: Journal of Molecular Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29145175/antimicrobial-activity-of-galangin-and-its-effects-on-murein-hydrolases-of-vancomycin-intermediate-staphylococcus-aureus-visa-strain-mu50
#12
Jing Ouyang, Fengjun Sun, Wei Feng, Yonghong Xie, Lijuan Ren, Yongchuan Chen
Backgroud: Antibiotic treatment for infections caused by vancomycin-intermediate Staphylococcus aureus (VISA) strains is challenging, and only a few effective and curative methods have been developed to combat these strains. This study aimed to investigate the antimicrobial activity of galangin against S. aureus and its effects on the murein hydrolases of VISA strain Mu50. This is the first report on these effects of galangin, and it may help to improve the treatment for VISA infections by demonstrating the effective use of galangin...
November 17, 2017: Chemotherapy
https://www.readbyqxmd.com/read/29145121/role-of-acid-alkali-treatment-in-primary-sludge-anaerobic-fermentation-insights-into-microbial-community-structure-functional-shifts-and-metabolic-output-by-high-throughput-sequencing
#13
Xiao Huang, Wenyi Dong, Hongjie Wang, Yangyang Feng
This study aimed to investigate the effect of acid- or alkali-treatment on volatile fatty acids (VFAs) production and microbiological mechanism during primary sludge anaerobic fermentation. Seven fermentation experiments were conducted at different pH (3-12). Results showed that the optimal pH was 10 for accumulation of VFAs. High-throughput sequencing results indicated that acid and alkali treatment could inhibit Erysipelotrichaceae_UCG-004 and norank_p_Aminicenantes, instead of promoting Pseudomonas and Tissierella at acidic and alkaline condition, respectively...
November 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145117/biodiesel-production-from-microbial-granules-in-sequencing-batch-reactor
#14
Lin Liu, Yuling Hong, Xin Ye, Lili Wei, Jie Liao, Xu Huang, Chaoxiang Liu
Effect of reaction variables of in situ transesterification on the biodiesel production, and the characteristic differences of biodiesel obtained from aerobic granular sludge (AG) and algae-bacteria granular consortia (AAG) were investigated. The results indicated that the effect of variables on the biodiesel yield decreased in the order of methanol quantity > catalyst concentration > reaction time, yet the parameters change will not significantly affect biodiesel properties. The maximum biodiesel yield of AAG was 66...
November 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29145102/remediation-of-a-soil-chronically-contaminated-with-hydrocarbons-through-persulfate-oxidation-and-bioremediation
#15
Rocío Medina, Pedro Maximiliano David Gara, Antonio José Fernández-González, Janina Alejandra Rosso, María Teresa Del Panno
The impact of remediation combining chemical oxidation followed by biological treatment on soil matrix and microbial community was studied, of a chronically hydrocarbon contaminated soil sourced from a landfarming treatment. Oxidation by ammonium persulfate produced a significant elimination of polycyclic aromatic hydrocarbons (PAHs) and an increase in PAH bioavailability. Organic-matter oxidation mobilized nutrients from the soil matrix. The bacterial populations were affected negatively, with a marked diminution in the diversity indices...
November 13, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29145084/low-voltage-electric-potential-as-a-driving-force-to-hinder-biofouling-in-self-supporting-carbon-nanotube-membranes
#16
Chidambaram Thamaraiselvan, Avner Ronen, Sofia Lerman, Moran Balaish, Yair Ein-Eli, Carlos G Dosoretz
This study aimed at evaluating the contribution of low voltage electric field, both alternating (AC) and direct (DC) currents, on the prevention of bacterial attachment and cell inactivation to highly electrically conductive self-supporting carbon nanotubes (CNT) membranes at conditions which encourage biofilm formation. A mutant strain of Pseudomonas putida S12 was used a model bacterium and either capacitive or resistive electrical circuits and two flow regimes, flow-through and cross-flow filtration, were studied...
November 3, 2017: Water Research
https://www.readbyqxmd.com/read/29145079/microbial-transcript-and-metabolome-analysis-uncover-discrepant-metabolic-pathways-in-autotrophic-and-mixotrophic-anammox-consortia
#17
Ying Feng, Yunpeng Zhao, Yongzhao Guo, Sitong Liu
The ability of anammox bacteria to utilize organic matter has drawn extensive attention. However, the metabolic discrepancies between autotrophic and mixotrophic anammox consortia need to be further explored. Here, microbial transcript and metabolomic analysis were conducted for the samples harvested in the reactors and batch assays to investigate the phenotype discrepancies and intrinsic causes in autotrophic and mixotrophic anammox consortia. Results showed that metabolically active community structures did not show significant difference between autotrophic and mixotrophic anammox consortia (C/N = 0...
October 31, 2017: Water Research
https://www.readbyqxmd.com/read/29145071/proteomic-analysis-reveals-that-tomato-interaction-with-plant-growth-promoting-bacteria-is-highly-determined-by-ethylene-perception
#18
Pablo Ibort, Hiroyuki Imai, Matsuo Uemura, Ricardo Aroca
Feeding an increasing global population as well as reducing environmental impact of crops is the challenge for the sustainable intensification of agriculture. Plant-growth-promoting bacteria (PGPB) management could represent a suitable method but elucidation of their action mechanisms is essential for a proper and effective utilization. Furthermore, ethylene is involved in growth and response to environmental stimuli but little is known about the implication of ethylene perception in PGPB activity. The ethylene-insensitive tomato never ripe and its isogenic wild-type cv...
November 10, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29145002/different-transport-behaviors-of-bacillus-subtilis-cells-and-spores-in-saturated-porous-media-implications-for-contamination-risks-associated-with-bacterial-sporulation-in-aquifer
#19
Qi Li, Jiakuan Yang, Wei Fan, Dandan Zhou, Xiaoyu Wang, Leilei Zhang, Mingxin Huo, John C Crittenden
In this study, we compared the transport characteristics of Bacillus subtilis cells and their spores in saturated porous media. Both the bacteria cells and spores were readily transported under lower ionic strengths (IS) (1mM), featuring high mass recovery rates (>75%). The bacteria cells recovery rate declined to 58.05% when the IS increased to 100mM; but the spores recovery rate remained at 74.54%. Spores appear to be more mobile than bacterial cells over wide range of IS. Surface properties and breakthrough curves analysis of the two bio-particles (cells and spores) indicated that attachment, straining and blocking mechanisms contribute differently to deposition processes...
November 11, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29144965/role-of-serum-1-3%C3%AE-d-glucan-assay-in-early-diagnosis-of-invasive-fungal-infections-in-a-neonatal-intensive-care-unit
#20
Abd Elazeez Shabaan, Lobna Mahmoud Elbaz, Wafaa M El-Emshaty, Basma Shouman
OBJECTIVES: To study the microbiological pattern of late onset neonatal sepsis cultures and to assess the diagnostic performance of serum (1,3)-β-d-glucan level for early diagnosis of invasive fungemia in high-risk infants admitted to a neonatal intensive care unit. METHODS: A prospective multicenter clinical trial conducted on infants at high risk for invasive fungal infections, with suspected late onset sepsis, admitted to a neonatal intensive care unit at Mansoura University Children's Hospital and Mansoura General Hospital between March 2014 and February 2016...
November 13, 2017: Jornal de Pediatria
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