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https://www.readbyqxmd.com/read/27922236/microorganisms-isolated-from-cultures-and-infection-focus-and-antibiotic-treatments-in-febrile-neutropenic-children-from-%C3%A5-anl%C3%A4-urfa-turkey
#1
Z Canan Özdemir, Ahmet Koç, Ali Ayçiçek
Chemotherapy induced febrile neutropenia predisposes patients to life threatening infections. We aimed to determine the causative microorganisms, infection focus and antibiotic treatment success in febrile neutropenic children with leukemia. A total of 136 febrile neutropenic episodes in 48 leukemic children were reviewed retrospectively from records. Among 136 febrile neutropenic episodes, 68 (50%) episodes were microbiologically documented. Methicillin sensitive coagulase (-) Staphylococcus aureus were the most common isolates from hemoculture (20...
2016: Turkish Journal of Pediatrics
https://www.readbyqxmd.com/read/27922070/deep-sequencing-of-the-16s-ribosomal-rna-of-the-neonatal-oral-microbiome-a-comparison-of-breast-fed-and-formula-fed-infants
#2
S S Al-Shehri, E L Sweeney, D M Cowley, H G Liley, P D Ranasinghe, B G Charles, P N Shaw, D Vagenas, J A Duley, C L Knox
In utero and upon delivery, neonates are exposed to a wide array of microorganisms from various sources, including maternal bacteria. Prior studies have proposed that the mode of feeding shapes the gut microbiota and, subsequently the child's health. However, the effect of the mode of feeding and its influence on the development of the neonatal oral microbiota in early infancy has not yet been reported. The aim of this study was to compare the oral microbiota of healthy infants that were exclusively breast-fed or formula-fed using 16S-rRNA gene sequencing...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27920781/inoculation-of-schizolobium-parahyba-with-mycorrhizal-fungi-and-plant-growth-promoting-rhizobacteria-increases-wood-yield-under-field-conditions
#3
Martha V T Cely, Marco A Siviero, Janaina Emiliano, Flávia R Spago, Vanessa F Freitas, André R Barazetti, Erika T Goya, Gustavo de Souza Lamberti, Igor M O Dos Santos, Admilton G De Oliveira, Galdino Andrade
Schizolobium parahyba var. amazonicum (Huber ex Ducke) occurs naturally in the Brazilian Amazon. Currently, it is being planted extensively because of its fast growth and excellent use in forestry. Consequently, there is great interest in new strategies to increase wood production. The interaction between soil microorganisms and plants, specifically in the roots, provides essential nutrients for plant growth. These interactions can have growth-promoting effects. In this way, this study assessed the effect of the inoculation with arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (PGPR) on growth of S...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27920653/intestinal-bacterial-infection-diagnosed-by-histological-examination-of-endoscopic-biopsy-specimens
#4
Michiko Yuki, Yuko Emoto, Katsuhiko Yoshizawa, Takashi Yuri, Airo Tsubura
Intestinal spirochetosis (IS) in humans is characterized by spirochetal microorganisms attached to the luminal surface of the colonic epithelium. In the present case, attached organisms appeared as 3- to 4 μm-thick (average thickness, 3.4 μm) basophilic fringes or haze in HE-stained endoscopic biopsy specimens. The basophilic fringes were clearly labeled by Treponema pallidum antiserum. Because IS is relatively rare in developed countries, thin basophilic fringes characteristic of IS are readily overlooked...
September 2016: Case Reports in Gastroenterology
https://www.readbyqxmd.com/read/27920489/bleb-related-infections-clinical-characteristics-risk-factors-and-outcomes-in-an-asian-population
#5
Zhu Li Yap, You Chuen Chin, Judy Yu-Fen Ku, Tat Keong Chan, Gillian Teh, Monisha Esther Nongpiur, Tin Aung, Shamira A Perera
PURPOSE: Comparison of the demographic, ocular, systemic and microbiological characteristics of eyes with bleb related infection (BRI) and bleb related endophthalmitis (BRE). METHODS: Retrospective chart review of patients with BRI from January 1996-July 2013. Identification done via the center's longstanding endophthalmitis audit, BRI audit and laboratory database identifying all conjunctival swabs from blebs. Blebitis was defined as anterior segment inflammation with mucopurulent material in or around the bleb, with anterior chamber cells but no hypopyon...
2016: Clinical Ophthalmology
https://www.readbyqxmd.com/read/27920430/biomimetic-antimicrobial-cloak-by-graphene-oxide-agar-hydrogel
#6
Massimiliano Papi, Valentina Palmieri, Francesca Bugli, Marco De Spirito, Maurizio Sanguinetti, Carlotta Ciancico, Maria Chiara Braidotti, Silvia Gentilini, Luca Angelani, Claudio Conti
Antibacterial surfaces have an enormous economic and social impact on the worldwide technological fight against diseases. However, bacteria develop resistance and coatings are often not uniform and not stable in time. The challenge is finding an antibacterial coating that is biocompatible, cost-effective, not toxic, and spreadable over large and irregular surfaces. Here we demonstrate an antibacterial cloak by laser printing of graphene oxide hydrogels mimicking the Cancer Pagurus carapace. We observe up to 90% reduction of bacteria cells...
December 2016: Scientific Reports
https://www.readbyqxmd.com/read/27920298/malate-synthase-and-%C3%AE-methylmalyl-coa-lyase-reactions-in-the-methylaspartate-cycle-in-haloarcula-hispanica
#7
Farshad Borjian, Jing Han, Jing Hou, Hua Xiang, Jan Zarzycki, Ivan A Berg
: Haloarchaea are extremely halophilic heterotrophic microorganisms belonging to the class Halobacteria (Euryarchaeota). Almost half of the haloarchaea possesses the genes coding for enzymes of the methylaspartate cycle, a recently discovered anaplerotic acetate assimilation pathway. In this cycle, the enzymes of the tricarboxylic acid cycle together with the dedicated enzymes of the methylaspartate cycle convert two acetyl-CoA molecules to malate. The methylaspartate cycle involves two reactions catalyzed by homologous enzymes belonging to the CitE-like enzyme superfamily, the malyl-CoA lyase/thioesterase (haloarchaeal malate synthase, hMS, Hah_2476 in Haloarcula hispanica) and the β-methylmalyl-CoA lyase (hMCL, Hah_1341)...
December 5, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27920297/role-of-ribonucleotide-reductase-on-bacillus-subtilis-stress-associated-mutagenesis
#8
Karla Viridiana Castro-Cerritos, Ronald E Yasbin, Eduardo A Robleto, Mario Pedraza-Reyes
: The Gram-positive microorganism Bacillus subtilis relies on a single class Ib ribonucleotide reductase (RNR) to generate 2' -deoxyribonucleotides (dNDPs) for DNA replication and repair. In this work, we investigated the influence of RNR levels on B. subtilis stationary-phase-associated mutagenesis (SPM). Since RNR is essential in this bacterium, we engineered a conditional mutant in the strain B. subtilis YB955 (hisC952, metB5, leu427) in which expression of the nrdEF operon was modulated by isopropyl-β-D-thiogalactopyranoside (IPTG)...
December 5, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27920201/the-streptococcus-gordonii-adhesin-csha-binds-host-fibronectin-via-a-catch-clamp-mechanism
#9
Catherine R Back, Maryta N Sztukowska, Marisa Till, Richard J Lamont, Howard F Jenkinson, Angela H Nobbs, Paul R Race
Adherence of bacteria to biotic or abiotic surfaces is a prerequisite for host colonization and represents an important step in microbial pathogenicity. This attachment is facilitated by bacterial adhesins at the cell surface. Due to their size and often elaborate multi-domain architectures, these polypeptides represent challenging targets for detailed structural and functional characterization. The multifunctional fibrillar adhesin CshA, which mediates binding to both host molecules and other microorganisms, is an important determinant of colonisation by Streptococcus gordonii, an oral commensal and opportunistic pathogen of animals and humans...
December 5, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27919743/dual-antiplasmodial-activity-of-vitamin-d3-and-its-analog-22-oxacalcitriol-by-direct-and-indirect-mechanisms
#10
Kiichi Yamamoto, Moritoshi Iwagami, Takenori Seki, Shigeyuki Kano, Nobuo Ota, Manabu Ato
Recent evidence suggests that 1α,25-dihydroxyvitamin D3 (calcitriol, VD3), the active form of vitamin D (VD), can inhibit the proliferation of microorganisms. In the present study, we conducted in vitro experiments and utilized in vivo murine models to investigate the antimalarial activity of VD3 and its analog, 22-oxacalcitriol (22-OCT), which was designed to cause less hypercalcemia than VD3. VD3 and 22-OCT treatments effectively resolved a Plasmodium chabaudi (Pc) infection in wild-type mice. Reduced parasitemia was observed during the acute phase of infection in the presence of VD3 and 22-OCT, followed by a delayed peak during the chronic stage of infection...
December 2, 2016: Parasitology International
https://www.readbyqxmd.com/read/27919704/global-transcriptome-analysis-of-halolamina-sp-to-decipher-the-salt-tolerance-in-extremely-halophilic-archaea
#11
Aslıhan Kurt-Kızıldoğan, Büşra Abanoz, Sezer Okay
Extremely halophilic archaea survive in the hypersaline environments such as salt lakes or salt mines. Therefore, these microorganisms are good sources to investigate the molecular mechanisms underlying the tolerance to high salt concentrations. In this study, a global transcriptome analysis was conducted in an extremely halophilic archaeon, Halolamina sp. YKT1, isolated from a salt mine in Turkey. A comparative RNA-seq analysis was performed using YKT1 isolate grown either at 2.7M NaCl or 5.5M NaCl concentrations...
December 2, 2016: Gene
https://www.readbyqxmd.com/read/27919512/effect-of-metronidazole-supplemented-with-hydroquinone-on-the-adhesion-of-lactobacillus-acidophilus-in-ovine-vaginal-cells
#12
Tamara L Coletti Zabala, María E Zerbatto, Elda B R Perotti, Ana M Smacchia, Adriana Ombrella, Alejandro R Pidello
This work demonstrates that the addition of metronidazole together with a ubiquitous quinone compound reduces adherence of Lactobacillus acidophilus to ovine vaginal cells. Spectrophotometric and voltammetric studies have shown that neoformed compounds were observed in these systems; there were also changes in their electroactive composition, and the oxidant status had a significantly higher value compared to the control (p<0.05). Based on reduction potential (E; mV), the distribution of electroactive compound concentrations suggests that the compounds with low reduction potential induce this behavior, which would indicate that the addition of metronidazole with a ubiquitous quinone compound to the vaginal system might increase the reductive capacity of these systems...
December 2, 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/27918973/long-term-effects-of-cupric-oxide-nanoparticles-cuo-nps-on-the-performance-microbial-community-and-enzymatic-activity-of-activated-sludge-in-a-sequencing-batch-reactor
#13
Sen Wang, Zhiwei Li, Mengchun Gao, Zonglian She, Bingrui Ma, Liang Guo, Dong Zheng, Yangguo Zhao, Chunji Jin, Xuejiao Wang, Feng Gao
The long-term effects of cupric oxide nanoparticles (CuO NPs) on the performance, microbial activity and microbial community of activated sludge were investigated in a sequencing batch reactor (SBR). The SBR performance had no evident change at 0-10 mg/L CuO NPs, whereas the CuO NPs concentration at 30-60 mg/L affected the COD, NH4(+)-N and soluble orthophosphate (SOP) removal, nitrogen and phosphorus removal rate and microbial enzymatic activity of activated sludge. Some CuO NPs might be absorbed on the surface of activated sludge or penetrate the microbial cytomembrane into the microbial cell interior of activated sludge...
December 2, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27918732/relationship-between-889-c-t-polymorphism-in-interleukin-1a-gene-and-risk-of-chronic-periodontitis-evidence-from-a-meta-analysis-with-new-published-findings
#14
F-R-P da Silva, A-C-C Guimarães-Vasconcelos, L-F de-Carvalho-França, D di-Lenardo, L-S Rodrigues, M-L-L Barreto-do-Nascimento, D-F-P Vasconcelos
BACKGROUND: Periodontitis results from an inflammatory response caused by accumulative microorganisms in periodontal sites. Several factors are involved in pathogenesis of periodontitis, for example the -889 C/T polymorphism in interleukin-1A gene. This study aimed to evaluate the relationship between this polymorphism and risk of development of chronic periodontitis by a meta-analysis based in new published findings. MATERIAL AND METHODS: Thereunto a review in literature was performed in the electronic biomedical and education databases (Cochrane Library, Google Scholar, MEDLINE and PubMed) to studies published before August 2, 2015, the abstracts were evaluated and the data extraction performed by two calibrated examiners...
December 6, 2016: Medicina Oral, Patología Oral y Cirugía Bucal
https://www.readbyqxmd.com/read/27918423/potentiometric-aptasensing-of-vibrio-alginolyticus-based-on-dna-nanostructure-modified-magnetic-beads
#15
Guangtao Zhao, Jiawang Ding, Han Yu, Tanji Yin, Wei Qin
A potentiometric aptasensing assay that couples the DNA nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of Vibrio alginolyticus is herein described. The DNA nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. The solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chronopotentiometrically detect the change in the charge or DNA concentration on the magnetic beads, which is induced by the interaction between Vibrio alginolyticus and the aptamer on the DNA nanostructures...
December 2, 2016: Sensors
https://www.readbyqxmd.com/read/27918295/a-new-antibacterial-silver-i-complex-incorporating-2-5-dimethylpyrazine-and-the-anti-inflammatory-diclofenac
#16
Sevim Hamamci Alisir, Necmi Dege
Ag(I)-containing coordination complexes have attracted attention because of their photoluminescence properties and antimicrobial activities and, in principle, these properties depend on the nature of the structural topologies. A novel two-dimensional silver(I) complex with the anti-inflammatory diclofenac molecule, namely bis{μ-2-[2-(2,6-dichloroanilino)phenyl]acetato-κ(3)O,O':O}bis(μ-2,5-dimethylpyrazine-κ(2)N:N')silver(I), [Ag2(C14H10Cl2NO2)2(C6H8N2)]n, (I), has been synthesized and characterized by single-crystal X-ray diffraction, revealing that the Ag(I) ions are chelated by the carboxylate groups of the anionic 2-[2-(2,6-dichloroanilino)phenyl]acetate (dicl) ligand in a μ3-η(1):η(2) coordination mode...
December 1, 2016: Acta Crystallographica. Section C, Structural Chemistry
https://www.readbyqxmd.com/read/27917968/innovations-in-food-preservation-in-pastoral-zones
#17
C Jans, D W Mulwa Kaindi, L Meile
Food preservation makes a significant contribution to food security and food safety in pastoral communities with limited access to external food sources. Raw materials are preserved by heating, drying, smoking, pickling, salting, curing or fermentation with microorganisms. This article describes preservation techniques in the pastoral context, targeting the major dietary components of milk, meat and cereals; related health risks; and potential innovations for food preservation. Sustainable elimination of pathogenic microorganisms, preventing re-contamination, sporulation and the growth of zoonotic and foodborne microorganisms, is necessary to enhance food safety and ensure food security by reducing post-harvest losses and food waste...
November 2016: Revue Scientifique et Technique
https://www.readbyqxmd.com/read/27917855/identification-of-microorganisms-on-mobile-phones-of-intensive-care-unit-health-care-workers-and-medical-students-in-the-tertiary-hospital
#18
Ivan Kotris, Domagoj Drenjančević, Jasminka Talapko, Suzana Bukovski
Aim To identify and investigate a difference between microorganisms present on intensive care unit (ICU) health care workers' (HCW, doctors, nurses or medical technicians) and medical students' mobile phones as well as to investigate a difference between the frequency and the way of cleaning mobile phones. Methods Fifty swabs were collected from HCWs who work in the ICU (University Hospital Centre Osijek) and 60 swabs from medical students (School of Medicine, University of Osijek). Microorganisms were identified according to standard microbiological methods and biochemical tests to the genus/species level...
February 1, 2017: Medicinski Glasnik
https://www.readbyqxmd.com/read/27917834/the-crystal-structure-of-the-endoglucanase-cel10-a-family-8-glycosyl-hydrolase-from-klebsiella-pneumoniae
#19
Ayman Attigani, Lifang Sun, Qing Wang, Yadan Liu, Dingping Bai, Shengping Li, Xiaohong Huang
Cellulases are produced by microorganisms that grow on cellulose biomass. Here, a cellulase, Cel10, was identified in a strain of Klebsiella pneumoniae isolated from Chinese bamboo rat gut. Analysis of substrate specificity showed that Cel10 is able to hydrolyze amorphous carboxymethyl cellulose (CMC) and crystalline forms of cellulose (Avicel and xylan) but is unable to hydrolyze p-nitrophenol β-D-glucopyranoside (p-NPG), proving that Cel10 is an endoglucanase. A phylogenetic tree analysis indicates that Cel10 belongs to the glycoside hydrolase 8 (GH8) subfamily...
December 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/27917719/prevention-and-treatment-of-postoperative-endophthalmitis-using-povidone-iodine
#20
Hiroyuki Shimada, Hiroyuki Nakashizuka, Andrzej Grzybowski
Postoperative endophthalmitis is typically caused by patient's conjunctival bacterial flora or contaminated solutions and instruments. Visual outcome is often poor in severe cases, and treatment is especially difficult when endophthalmitis is caused by multidrug resistant bacteria. Povidone-iodine is used worldwide due to its wide-spectrum antimicrobial activity, absence of resistant bacteria to povidone-iodine, and low cost. Furthermore, the effective concentration against microorganisms and safe concentration for ocular tissues have been well established...
December 4, 2016: Current Pharmaceutical Design
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