keyword
https://read.qxmd.com/read/37536622/anandamide-reduces-the-migration-of-lymphocytes-to-the-intestine-by-cb2-activation-and-reduces-tnf-%C3%AE-in-the-target-organs-protecting-mice-from-graft-versus-host-disease
#21
JOURNAL ARTICLE
Bárbara Betônico Berg, Ana Flávia Santos Linhares, Daniel Messias Martins, Milene Alvarenga Rachid, Stêfany Bruno de Assis Cau, Giovane Galdino de Souza, Jonatan Constança Silva de Carvalho, Carlos Arterio Sorgi, Thiago Roberto Lima Romero, Vanessa Pinho, Mauro Martins Teixeira, Marina Gomes Miranda E Castor
Graft-versus-host disease (GVHD) is a serious inflammatory illness that often occurs as a secondary complication of bone marrow transplantation. Current therapies have limited effectiveness and fail to achieve a balance between inflammation and the graft-versus-tumor effect. In this study, we investigate the effects of the endocannabinoid anandamide on the complex pathology of GVHD. We assess the effects of an irreversible inhibitor of fatty acid amine hydrolase or exogenous anandamide and find that they increase survival and reduce clinical signs in GVHD mice...
August 1, 2023: European Journal of Pharmacology
https://read.qxmd.com/read/37511839/an-activation-specific-anti-mac-1-designed-ankyrin-repeat-protein-attenuates-colitis-in-mice
#22
JOURNAL ARTICLE
Istvan Bojti, Qianqi Wang, Tibor Bojti, Felicitas Bojti, Patrick Malcolm Siegel, Timo Heidt, Martin Moser, Christoph Bode, Dirk Westermann, Karlheinz Peter, Philipp Diehl
(1) Background: Inflammatory bowel diseases are complex and multifactorial disorders of unknown etiology. The extravasation of activated leukocytes is a critical step in the pathogenesis of these diseases. Leukocyte integrin Mac-1 (αM β2 ; CD11b/CD18) is crucial for the extravasation of myeloid cells, and a novel activation-specific anti-Mac-1 Designed Ankyrin Repeat protein (DARPin F7) is a promising therapeutic agent for inflammatory diseases. In its activated conformation, Mac-1 expresses the high-affinity binding site I-domain, which the DARPin F7 selectively targets...
June 28, 2023: Life
https://read.qxmd.com/read/37492529/dual-wave-of-neutrophil-recruitment-determines-the-outcome-of-c-albicans-infection
#23
JOURNAL ARTICLE
Weiwei Zhu, Huifang Zhang, Qiming Dong, Hongyong Song, Lin Zhao
Candida albicans is a ubiquitous fungus that can cause superficial and systemic infections in humans. Neutrophils play a crucial role in controlling C. albicans infections. When C. albicans enters the bloodstream, it tends to get trapped in capillary vessels. However, the behavior of neutrophils in combating capillary-residing fungi has not been fully characterized. In this study, we used transgenic mice and whole mount imaging to investigate the growth of C. albicans and its interaction with innate immune cells in different organs...
2023: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/37310029/decrypting-integrins-by-mixed-solvent-molecular-dynamics-simulations
#24
JOURNAL ARTICLE
Ioana M Ilie, Claus Ehrhardt, Amedeo Caflisch, Gabriele Weitz-Schmidt
Integrins are a family of α/β heterodimeric cell surface adhesion receptors which are capable of transmitting signals bidirectionally across membranes. They are known for their therapeutic potential in a wide range of diseases. However, the development of integrin-targeting medications has been impacted by unexpected downstream effects including unwanted agonist-like effects. Allosteric modulation of integrins is a promising approach to potentially overcome these limitations. Applying mixed-solvent molecular dynamics (MD) simulations to integrins, the current study uncovers hitherto unknown allosteric sites within the integrin α I domains of LFA-1 (αL β2 ; CD11a/CD18), VLA-1 (α1 β1 ; CD49a/CD29), and Mac-1 (αM β2 , CD11b/CD18)...
June 13, 2023: Journal of Chemical Information and Modeling
https://read.qxmd.com/read/37275893/the-complement-receptor-c5ar2-regulates-neutrophil-activation-and-function-contributing-to-neutrophil-driven-epidermolysis-bullosa-acquisita
#25
JOURNAL ARTICLE
Daniel L Seiler, Katja H Kähler, Marie Kleingarn, Christian D Sadik, Katja Bieber, Jörg Köhl, Ralf J Ludwig, Christian M Karsten
INTRODUCTION: The function of the second receptor for the complement cleavage product C5a, C5aR2, is poorly understood and often neglected in the immunological context. Using mice with a global deficiency of C5aR2 , we have previously reported an important role of this receptor in the pathogenesis of the neutrophil-driven autoimmune disease epidermolysis bullosa acquisita (EBA). Based on in vitro analyses, we hypothesized that the absence of C5aR2 specifically on neutrophils is the cause of the observed differences...
2023: Frontiers in Immunology
https://read.qxmd.com/read/37195185/c3-cd11b-mediated-leishmania-major-internalization-by-neutrophils-induces-intraphagosomal-nox2-mediated-respiratory-burst-but-fails-to-eliminate-parasites-and-induces-a-state-of-stalled-apoptosis
#26
JOURNAL ARTICLE
Adam J Ranson, Matheus B Carneiro, Benjamin Perks, Robert Penner, Leon Melo, Johnathan Canton, Jackson Egen, Nathan C Peters
Recruited neutrophils are among the first phagocytic cells to interact with the phagosomal pathogen Leishmania following inoculation into the mammalian dermis. Analysis of Leishmania-infected neutrophils has revealed alterations in neutrophil viability, suggesting that the parasite can both induce or inhibit apoptosis. In this study, we demonstrate that entry of Leishmania major into murine neutrophils is dependent on the neutrophil surface receptor CD11b (CR3/Mac-1) and is enhanced by parasite opsonization with C3...
May 17, 2023: Journal of Immunology
https://read.qxmd.com/read/37125077/complement-dependent-tnf%C3%AE-production-in-neutrophil-like-hl60%C3%A2-cells
#27
JOURNAL ARTICLE
Hiroyuki Tabata, Hiroyuki Morita, Kenichi Kouyama, Yumi Tohyama
Neutrophils develop in the bone marrow (BM) from hematopoietic stem cells (HSCs) through a series of progenitor cells and mature neutrophils play a critical role in the human immune system. Previous studies revealed that tumor necrosis factor α (TNFα) produced by immature neutrophils contributes to HSCs development and vascular regeneration in the BM niche. However, the precise mechanism of TNFα production in immature neutrophils remains unclear. This study aims to assess the relationship between complement C3 activation and TNFα production from immature neutrophils...
July 2023: Biochemistry and Biophysics Reports
https://read.qxmd.com/read/37105254/synthesis-of-corn-bract-cellulose-based-au-3-fluorescent-probe-and-its-application-in-composite-membranes
#28
JOURNAL ARTICLE
Xushuo Yuan, Wentao Yao, Decai Ji, Li Liu, Yanfei Lin, Heyang Zeng, Tao Jin, Kaimeng Xu, Guanben Du, Lianpeng Zhang
To achieve real-time monitoring of Au3+ , a corn bract cellulose-based fluorescent probe MAC-1 for was synthesized. MAC-1 showed good fluorescence properties in DMF-H2 O (1:9, v/v, pH = 7.4) solution, showed a fluorescence emission peak at 520 nm with quenching fluorescence properties for Au3+ . The structure of MAC-1 was analyzed by SEM (Sample microstructure images), XRD (X-ray diffraction), FTIR (Fourier transform infrared spectroscopy), 1 H NMR, Elemental analysis, EDS, Mapping and TG (Thermogravimetry) were analyzed...
April 25, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/37021588/mechano-redox-control-of-mac-1-de-adhesion-by-pdi-promotes-directional-movement-under-flow
#29
JOURNAL ARTICLE
Alexander Dupuy, Camilo Aponte-Santamaría, Adva Yeheskel, Elinor Hortle, Stefan H Oehlers, Frauke Gräter, Philip J Hogg, Freda H Passam, Joyce Chiu
BACKGROUND: Neutrophil migration is critical to the initiation and resolution of inflammation. Macrophage-1 antigen (Mac-1; CD11b/CD18, αMβ2) is a leukocyte integrin essential for firm adhesion to endothelial ICAM-1 (intercellular adhesion molecule 1) and migration of neutrophils in the shear forces of the circulation. PDI (protein disulfide isomerase) has been reported to influence neutrophil adhesion and migration. We aimed to elucidate the molecular mechanism of PDI control of Mac-1 affinity for ICAM-1 during neutrophil migration under fluid shear...
April 28, 2023: Circulation Research
https://read.qxmd.com/read/37014068/therapeutic-effect-of-two-strategies-directed-at-disruption-of-pathogenic-neutrophil-extracellular-vesicles-in-a-murine-emphysema-model
#30
JOURNAL ARTICLE
Kristopher R Genschmer, Matthew Madison, Liliana Viera, Camilla Margaroli, Amit Gaggar, J Edwin Blalock, Derek W Russell
Chronic obstructive pulmonary disease (COPD) is characterized by lung extracellular matrix (ECM) remodeling which contributes to obstruction. This is driven, in part by extracellular vesicles (EVs) from activated neutrophils (PMNs), which express on their surface an α-1 antitrypsin (AAT) insensitive form of neutrophil elastase (NE). These EVs are predicted to bind to collagen fibers via Mac-1 integrins, during which time NE can enzymatically degrade the collagen. Protamine sulfate (PS), a cationic compound used safely for decades in humans, has been shown, in vitro, to dissociate this NE from the EV surface, rendering it AAT-sensitive...
April 4, 2023: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://read.qxmd.com/read/36923240/cathelicidin-promotes-liver-repair-after-acetaminophen-induced-liver-injury-in-mice
#31
JOURNAL ARTICLE
Tingting Zhai, Jingjing Zhang, Jie Zhang, Bilian Liu, Zhiguang Zhou, Feng Liu, Yan Wu
BACKGROUND & AIMS: Acetaminophen (APAP)-induced acute liver injury (AILI) is a leading cause of acute liver failure (ALF). N -acetylcysteine (NAC) is only effective within 24 h after APAP intoxication, raising an urgent need for alternative approaches to treat this disease. This study aimed to test whether cathelicidin ( Camp ), which is a protective factor in chronic liver diseases, protects mice against APAP-induced liver injury and ALF. METHODS: A clinically relevant AILI model and an APAP-induced ALF model were generated in mice...
April 2023: JHEP reports: innovation in hepatology
https://read.qxmd.com/read/36894025/hypoxia-induced-vascular-remodeling-responses-in-the-brain-are-much-more-robust-than-other-organs
#32
JOURNAL ARTICLE
Arjun Sapkota, Sebok K Halder, Richard Milner
Exposure to chronic mild hypoxia (CMH; 8-10 % O2 ) promotes a robust vascular remodeling response in the brain resulting in 50 % increased vessel density over a period of two weeks. It is currently unknown whether blood vessels in other organs show similar responses. To address this question, mice were exposed to CMH for 4 days and various markers of vascular remodeling were examined in the brain along with heart, skeletal muscle, kidney, and liver. In contrast to brain, where CMH strongly promoted endothelial proliferation, none of the peripheral organs showed this response and in heart and liver, CMH notably reduced endothelial proliferation...
March 7, 2023: Microvascular Research
https://read.qxmd.com/read/36868231/mac-1-marks-a-quiescent-and-functionally-superior-hsc-subset-during-regeneration
#33
JOURNAL ARTICLE
Anna Rydström, Els Mansell, Valgardur Sigurdsson, Julia Sjöberg, Shamit Soneji, Kenichi Miharada, Jonas Larsson
Mouse hematopoietic stem cells (HSCs) have been extensively defined both molecularly and functionally at steady state, while regenerative stress induces immunophenotypical changes that limit high purity isolation and analysis. It is therefore important to identify markers that specifically label activated HSCs to gain further knowledge about their molecular and functional properties. Here, we assessed the expression of macrophage-1 antigen (MAC-1) on HSCs during regeneration following transplantation and observed a transient increase in MAC-1 expression during the early reconstitution phase...
February 13, 2023: Stem Cell Reports
https://read.qxmd.com/read/36818775/phenotyping-and-genotyping-of-hna-prevalence-risk-of-alloimmunization-and-hna-incompatibilities-in-indians
#34
JOURNAL ARTICLE
Harita Gogri, Meghana Parihar, Swati Kulkarni, Manisha Madkaikar, Jayashree Sharma, Ajit Gorakshakar
BACKGROUND: Antibodies to human neutrophil alloantigens (HNA) are involved in the pathophysiology of several clinical conditions including transfusion-related acute lung injury (TRALI), alloimmune and autoimmune neutropenia, and febrile nonhemolytic transfusion reactions leading to neutropenia. The cognate antigens are polymorphic structures expressed on several glycoproteins on the neutrophils, i.e., antigens HNA-1a, -1b, -1c, and -1d on Fc-γ-receptor IIIb; HNA-2 on CD177; HNA-3a and -3b on choline transporter-like protein 2; HNA-4a and -4b on CD11b/αM subunit of the αMβ2-integrin (CD11b/CD18, Mac-1, CR3); and HNA-5a and -5b on αL-subunit (CD11a) of the αLβ2 integrin (CD11a/CD18), leukocyte function associated molecule (LFA)-1...
February 2023: Transfusion Medicine and Hemotherapy
https://read.qxmd.com/read/36801813/proteinase-3-promotes-formation-of-multinucleated-giant-cells-and-granuloma-like-structures-in-patients-with-granulomatosis-with-polyangiitis
#35
JOURNAL ARTICLE
Scott R Henderson, Harry Horsley, Paul Frankel, Maryam Khosravi, Talya Goble, Stephen Carter, Marilina Antonelou, Rhys D R Evans, Xiang Zhang, Tai-Ying Chu, Hsi-Hsien Lin, Siamon Gordon, Alan David Salama
OBJECTIVES: Granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA) are autoimmune vasculitides associated with antineutrophil cytoplasm antibodies that target proteinase 3 (PR3) or myeloperoxidase (MPO) found within neutrophils and monocytes. Granulomas are exclusively found in GPA and form around multinucleated giant cells (MGCs), at sites of microabscesses, containing apoptotic and necrotic neutrophils. Since patients with GPA have augmented neutrophil PR3 expression, and PR3-expressing apoptotic cells frustrate macrophage phagocytosis and cellular clearance, we investigated the role of PR3 in stimulating giant cell and granuloma formation...
June 2023: Annals of the Rheumatic Diseases
https://read.qxmd.com/read/36796515/the-cis-association-of-cd47-with-integrin-mac-1-regulates-macrophage-responses-by-stabilizing-the-extended-integrin-conformation
#36
JOURNAL ARTICLE
Nataly P Podolnikova, Shundene Key, Xu Wang, Tatiana P Ugarova
CD47 is a ubiquitously expressed cell surface integrin-associated protein. Recently, we have demonstrated that integrin Mac-1 (αM β2 , CD11b/CD18, CR3), the major adhesion receptor on the surface of myeloid cells, can be coprecipitated with CD47. However, the molecular basis for the CD47-Mac-1 interaction and its functional consequences remain unclear. Here, we demonstrated that CD47 regulates macrophage functions directly interacting with Mac-1. In particular, adhesion, spreading, migration, phagocytosis, and fusion of CD47-deficient macrophages were significantly decreased...
February 14, 2023: Journal of Biological Chemistry
https://read.qxmd.com/read/36782240/comparison-of-2-anesthetic-protocols-and-surgical-timing-during-cesarean-section-on-neonatal-vitality-and-umbilical-cord-blood-parameters
#37
JOURNAL ARTICLE
Agnieszka Antończyk, Zdzisław Kiełbowicz, Wojciech Niżański, Małgorzata Ochota
BACKGROUND: The objective of this study was to evaluate the relationship between the mode of anesthesia, the time form the induction to the extraction of a puppy and the immediate postnatal vitality and umbilical cord blood gases parameters in cesarean section derived-puppies. Two different anesthetic protocols were used: inhalation using isoflurane (ISO) and combined-inhalation and epidural (EPI) with propofol being the induction agent. RESULTS: Significant differences were found in ISO group in pH values, pCO2 levels and Apgar scores between puppies at different extraction times (< 30 vs...
February 13, 2023: BMC Veterinary Research
https://read.qxmd.com/read/36769149/monocyte-derived-mirna-1914-5p-attenuates-il-1%C3%AE-induced-monocyte-adhesion-and-transmigration
#38
JOURNAL ARTICLE
Kohki Toriuchi, Toshie Kihara, Hiromasa Aoki, Hiroki Kakita, Satoru Takeshita, Hiroko Ueda, Yasumichi Inoue, Hidetoshi Hayashi, Yohei Shimono, Yasumasa Yamada, Mineyoshi Aoyama
Atherosclerosis can lead to cardiovascular and cerebrovascular diseases. Atherosclerotic plaque formation is promoted by the accumulation of inflammatory cells. Therefore, modulating monocyte recruitment represents a potential therapeutic strategy. In an inflammatory state, the expression of adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) is upregulated in endothelial cells. We previously reported that miR-1914-5p in endothelial cells suppresses interleukin (IL)-1β-induced ICAM-1 expression and monocyte adhesion to endothelial cells...
February 1, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/36702542/foxo3a-acts-as-immune-response-modulator-in-human-virus-negative-inflammatory-cardiomyopathy
#39
JOURNAL ARTICLE
Kamila Makrutzki-Zlotek, Felicitas Escher, Zehra Karadeniz, Ganna Aleshcheva, Heiko Pietsch, Konstanze Küchler, Heinz-Peter Schultheiss, Bettina Heidecker, Wolfgang Poller, Ulf Landmesser, Carmen Scheibenbogen, Tharusan Thevathasan, Carsten Skurk
OBJECTIVE: Inflammatory cardiomyopathy is characterised by inflammatory infiltrates leading to cardiac injury, left ventricular (LV) dilatation and reduced LV ejection fraction (LVEF). Several viral pathogens and autoimmune phenomena may cause cardiac inflammation.The effects of the gain of function FOXO3A single-nucleotide polymorphism (SNP) rs12212067 on inflammation and outcome were studied in a cohort of patients with inflammatory dilated cardiomyopathy (DCMi) in relation to cardiac viral presence...
January 26, 2023: Heart
https://read.qxmd.com/read/36696377/the-q163c-q309c-mutant-of-%C3%AE-mi-domain-is-an-active-variant-suitable-for-nmr-characterization
#40
JOURNAL ARTICLE
Hoa Nguyen, Tianwei Jing, Xu Wang
Integrin αMβ2 (Mac-1, CD11b/CD18, CR3) is an important adhesion receptor expressed on monocytes. Mac-1 is responsible for mediating cell migration, phagocytosis, degranulation as well as cell-cell fusion. It is also the most promiscuous integrin in terms of ligand specificity with over 100 ligands, most of which use the αMI-domain as their binding site. Despite the importance of αMI-domain in defining ligand interactions of Mac-1, structural studies of αMI-domain's interactions with ligands are lacking...
2023: PloS One
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