keyword
MENU ▼
Read by QxMD icon Read
search

CD18

keyword
https://www.readbyqxmd.com/read/27853810/inflammation-but-not-recruitment-of-adipose-tissue-macrophages-requires-signalling-through-mac-1-cd11b-cd18-in-diet-induced-obesity-dio
#1
Dennis Wolf, Nora Bukosza, David Engel, Marjorie Poggi, Felix Jehle, Nathaly Anto Michel, Yung-Chih Chen, Christian Colberg, Natalie Hoppe, Bianca Dufner, Louis Boon, Hermann Blankenbach, Ingo Hilgendorf, Constantin von Zur Muhlen, Jochen Reinöhl, Björn Sommer, Timoteo Marchini, Mark A Febbraio, Christian Weber, Christoph Bode, Karlheinz Peter, Esther Lutgens, Andreas Zirlik
Cell accumulation is a prerequisite for adipose tissue inflammation. The leukocyte integrin Mac-1 (CD11b/CD18, αMβ2) is a classic adhesion receptor critically regulating inflammatory cell recruitment. Here, we tested the hypothesis that a genetic deficiency and a therapeutic modulation of Mac-1 regulate adipose tissue inflammation in a mouse model of diet-induced obesity (DIO). C57Bl6/J mice genetically deficient (Mac-1(-/-)) or competent for Mac-1 (WT) consumed a high fat diet for 20 weeks. Surprisingly, Mac-1(-/-) mice presented with increased diet-induced weight gain, decreased insulin sensitivity in skeletal muscle and in the liver in insulin-clamps, insulin secretion deficiency and elevated glucose levels in fasting animals, and dyslipidaemia...
November 17, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27852744/integrin-cross-talk-regulates-the-human-neutrophil-response-to-fungal-%C3%AE-glucan-in-the-context-of-the-extracellular-matrix-a-prominent-role-for-vla3-in-the-antifungal-response
#2
Courtney M Johnson, Xian M O'Brien, Angel S Byrd, Valentina E Parisi, Alex J Loosely, Wei Li, Hadley Witt, Hafeez M Faridi, Craig T Lefort, Vineet Gupta, Minsoo Kim, Jonathan S Reichner
Candida albicans infection produces elongated hyphae resistant to phagocytic clearance compelling alternative neutrophil effector mechanisms to destroy these physically large microbial structures. Additionally, all tissue-based neutrophilic responses to fungal infections necessitate contact with the extracellular matrix (ECM). Neutrophils undergo a rapid, ECM-dependent mechanism of homotypic aggregation and NETosis in response to C. albicans mediated by the β2 integrin, complement receptor 3 (CR3, CD11b/CD18, αMβ2)...
November 16, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27828665/aerobic-training-modulates-t-cell-activation-in-elderly-women-with-knee-osteoarthritis
#3
W F Gomes, A C R Lacerda, G E A Brito-Melo, S F Fonseca, E Rocha-Vieira, A A O Leopoldino, M R Amorim, V A Mendonça
Osteoarthritis of the knee (kOA) is a disease that mainly affects the elderly and can lead to major physical and functional limitations. However, the specific effects of walking, particularly on the immune system, are unknown. Therefore, this study aimed to analyze the effect of 12 weeks of walking (3×/week) on the leukocyte profile and quality of life (QL) of elderly women with kOA. Sixteen women (age: 67±4 years, body mass index: 28.07±4.16 kg/m2) participated in a walking program. The variables were assessed before and after 12 weeks of training with a progressively longer duration (30-55 min) and higher intensity (72-82% of HRmax determined using a graded incremental treadmill test)...
2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/27815445/neutrophil-necroptosis-is-triggered-by-ligation-of-adhesion-molecules-following-gm-csf-priming
#4
Xiaoliang Wang, Zhaoyue He, He Liu, Shida Yousefi, Hans-Uwe Simon
Apoptosis is the most common form of neutrophil death under both physiological and inflammatory conditions. However, forms of nonapoptotic neutrophil death have also been observed. In the current study, we report that human neutrophils undergo necroptosis after exposure to GM-CSF followed by the ligation of adhesion receptors such as CD44, CD11b, CD18, or CD15. Using a pharmacological approach, we demonstrate the presence of a receptor-interacting protein kinase-3 (RIPK3)-a mixed lineage kinase-like (MLKL) signaling pathway in neutrophils which, following these treatments, first activates p38 MAPK and PI3K, that finally leads to the production of high levels of reactive oxygen species (ROS)...
November 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27799556/precise-gene-editing-paves-the-way-for-derivation-of-mannheimia-haemolytica-leukotoxin-resistant-cattle
#5
Sudarvili Shanthalingam, Ahmed Tibary, Jonathan E Beever, Poothapillai Kasinathan, Wendy C Brown, Subramaniam Srikumaran
Signal peptides of membrane proteins are cleaved by signal peptidase once the nascent proteins reach the endoplasmic reticulum. Previously, we reported that, contrary to the paradigm, the signal peptide of ruminant CD18, the β subunit of β2 integrins, is not cleaved and hence remains intact on mature CD18 molecules expressed on the surface of ruminant leukocytes. Leukotoxin secreted by Mannheimia (Pasteurella) haemolytica binds to the intact signal peptide and causes cytolysis of ruminant leukocytes, resulting in acute inflammation and lung tissue damage...
October 31, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27796667/lymphocyte-integrin-expression-differences-between-sirs-and-sepsis-patients
#6
D S Heffernan, S F Monaghan, Alfred Ayala
BACKGROUND: Systemic Inflammatory Response Syndrome (SIRS) and sepsis remain leading causes of death. Despite many similarities, the two entities are very distinct clinically and immunologically. T-Lymphocytes play a key pivotal role in the pathogenesis and ultimately outcome following both SIRS and sepsis. Integrins are essential in the trafficking and migration of lymphocytes. They also serve vital roles in efficient wound healing and clearance of infections. Here, we investigate whether integrin expression, specifically β1 (CD29) and β2 (CD18), are disrupted in SIRS and sepsis, and assess differences in integrin expression between these two critically ill clinical categories...
October 31, 2016: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/27779362/role-of-monocyte-recruitment-in-hemangiosarcoma-metastasis-in-dogs
#7
D P Regan, A Escaffi, J Coy, J Kurihara, S W Dow
Canine hemangiosarcoma (HSA) is a highly malignant tumour associated with short survival times because of early and widespread metastasis. In humans and rodents, monocytes play key roles in promoting tumour metastasis through stimulating tumour cell extravasation, seeding, growth and angiogenesis. Therefore, we investigated the potential association between monocyte infiltration and tumour metastasis in HSA and other common canine tumours. Immunohistochemistry was used to quantify CD18(+) monocytes within metastases...
October 25, 2016: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/27765364/phyllanthin-from-phyllanthus-amarus-inhibits-cellular-and-humoral-immune-responses-in-balb-c-mice
#8
Menaga Ilangkovan, Ibrahim Jantan, Syed Nasir Abbas Bukhari
BACKGROUND: Phyllanthin found in many Phyllanthus species has various biochemical and pharmacological properties especially on its hepatoprotective effects. However, its effect on the immune system has not been well documented. PURPOSE: In the present study, phyllanthin isolated from Phyllanthus amarus was investigated for its immunosuppressive effects on various cellular and humoral immune responses in Balb/C mice. METHODS: Male mice were treated daily at 20, 40 and 100mg/kg of phyllanthin for 14 days by oral gavage...
November 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27737899/am80-gcsf-synergizes-myeloid-expansion-and-differentiation-to-generate-functional-neutrophils-that-reduce-neutropenia-associated-infection-and%C3%A2-mortality
#9
Lin Li, Xiaotian Qi, Weili Sun, Hisham Abdel-Azim, Siyue Lou, Hong Zhu, Nemani V Prasadarao, Alice Zhou, Hiroyuki Shimada, Koichi Shudo, Yong-Mi Kim, Sajad Khazal, Qiaojun He, David Warburton, Lingtao Wu
Neutrophils generated by granulocyte colony-stimulating factor (GCSF) are functionally immature and, consequently, cannot effectively reduce infection and infection-related mortality in cancer chemotherapy-induced neutropenia (CCIN). Am80, a retinoic acid (RA) agonist that enhances granulocytic differentiation by selectively activating transcription factor RA receptor alpha (RARα), alternatively promotes RA-target gene expression. We found that in normal and malignant primary human hematopoietic specimens, Am80-GCSF combination coordinated proliferation with differentiation to develop complement receptor-3 (CR3)-dependent neutrophil innate immunity, through altering transcription of RA-target genes RARβ2, C/EBPε, CD66, CD11b, and CD18 This led to generation of functional neutrophils capable of fighting infection, whereas neutralizing neutrophil innate immunity with anti-CD18 antibody abolished neutrophil bactericidal activities induced by Am80-GCSF Further, Am80-GCSF synergy was evaluated using six different dose-schedule-infection mouse CCIN models...
November 2, 2016: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/27728815/fibrinogen-scaffolds-with-immunomodulatory-properties-promote-in%C3%A2-vivo-bone-regeneration
#10
Daniel M Vasconcelos, Raquel M Gonçalves, Catarina R Almeida, Inês O Pereira, Marta I Oliveira, Nuno Neves, Andreia M Silva, António C Ribeiro, Carla Cunha, Ana R Almeida, Cristina C Ribeiro, Ana M Gil, Elisabeth Seebach, Katharina L Kynast, Wiltrud Richter, Meriem Lamghari, Susana G Santos, Mário A Barbosa
The hypothesis behind this work is that fibrinogen (Fg), classically considered a pro-inflammatory protein, can promote bone repair/regeneration. Injury and biomaterial implantation naturally lead to an inflammatory response, which should be under control, but not necessarily minimized. Herein, porous scaffolds entirely constituted of Fg (Fg-3D) were implanted in a femoral rat bone defect and investigated at two important time points, addressing the bone regenerative process and the local and systemic immune responses, both crucial to elucidate the mechanisms of tissue remodelling...
December 2016: Biomaterials
https://www.readbyqxmd.com/read/27722179/production-and-purification-of-high-titer-foamy-virus-vector-for-the-treatment-of-leukocyte-adhesion-deficiency
#11
Md Nasimuzzaman, Danielle Lynn, Rebecca Ernst, Michele Beuerlein, Richard H Smith, Archana Shrestha, Scott Cross, Kevin Link, Carolyn Lutzko, Diana Nordling, David W Russell, Andre Larochelle, Punam Malik, Johannes C M Van der Loo
Compared to other integrating viral vectors, foamy virus (FV) vectors have distinct advantages as a gene transfer tool, including their nonpathogenicity, the ability to carry larger transgene cassettes, and increased stability of virus particles due to DNA genome formation within the virions. Proof of principle of its therapeutic utility was provided with the correction of canine leukocyte adhesion deficiency using autologous CD34(+) cells transduced with FV vector carrying the canine CD18 gene, demonstrating its long-term safety and efficacy...
2016: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/27720134/l-plastin-regulates-the-stability-of-the-immune-synapse-of-naive-and-effector-t-cells
#12
Guido Wabnitz, Emre Balta, Yvonne Samstag
T-cells need to be tightly regulated during their activation and effector phase to assure an appropriate defence against cancer or pathogens and - vice versa - to avoid autoimmune reactions. Regulatory signals are provided via the immune synapse between T-cells and antigen-presenting cells (APCs) or target cells. The stability and kinetics of immune synapse formation is critical for proper T-cell functions. It requires dynamic rearrangements of the actin cytoskeleton necessary for organized spatio-temporal redistribution of receptors and adhesion molecules...
September 27, 2016: Advances in Biological Regulation
https://www.readbyqxmd.com/read/27698655/neutrophil-integrins-and-matrix-ligands-and-net-release
#13
Xian M O'Brien, Jonathan S Reichner
Neutrophils are motile and responsive to tissue injury and infection. As neutrophils emigrate from the bloodstream and migrate toward a site of affliction, they encounter the tissue extracellular matrix (ECM) and thereby engage integrins. Our laboratory studies the neutrophilic response to the fungal pathogen Candida albicans either in the filamentous state of the microbe or to the purified pathogen-associated molecular pattern, β-glucan. We have gained an appreciation for the role of integrins in regulating the neutrophil anti-Candida response and how the presence or absence of ECM can drive experimental outcome...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27663487/in-vitro-complement-activation-adherence-to-red-blood-cells-and-induction-of-mononuclear-cell-cytokine-production-by-four-strains-of-aggregatibacter-actinomycetemcomitans-with-different-fimbriation-and-expression-of-leukotoxin
#14
C Damgaard, J Reinholdt, Y Palarasah, C Enevold, C Nielsen, M K Brimnes, P Holmstrup, C H Nielsen
BACKGROUND AND OBJECTIVE: The periodontal pathogen Aggregatibacter actinomycetemcomitans has been proposed as pro-atherogenic, and complement-mediated adherence to red blood cells (RBCs) may facilitate its systemic spread. We investigated the ability of four strains of A. actinomycetemcomitans with differential expression of leukotoxin A (LtxA) and fimbriae to activate complement, adhere to RBCs and elicit cytokine responses by mononuclear cells (MNCs). MATERIAL AND METHODS: Aggregatibacter actinomycetemcomitans serotype b strains HK 921, HK 1651, HK 2092 and HK 2108 were fluorescence-labeled, incubated with human whole blood cells in the presence of autologous serum, and assessed for RBC adherence by flow cytometry and for capacity to induce cytokine production by cytometric bead array analysis...
September 24, 2016: Journal of Periodontal Research
https://www.readbyqxmd.com/read/27652018/development-of-a-fast-and-economical-genotyping-protocol-for-bovine-leukocyte-adhesion-deficiency-blad-in-cattle
#15
Rafeeque R Alyethodi, Umesh Singh, Sushil Kumar, Rajib Deb, Rani Alex, Sheetal Sharma, Gyanendra S Sengar, B Prakash
Fast and economical means of assaying SNP's are important in diagnostic assays, especially when a large number of animals have to be screened for a genetic disease. This study was aimed at the development of a fast and economical screening assay for bovine leukocyte adhesion deficiency (BLAD) which is an important genetic disease of cattle industry. Four primers were designed where the outer primers amplify a 354 bp amplicon of CD18 gene carrying the polymorphism responsible for BLAD. The specifically designed inner primers in conjunction with the modified reaction mixture and cyclic conditions ensured amplification of either of wild or mutated alleles...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27606963/neutrophil-receptor-response-to-bacterial-n-formyl-peptides-is-similar-in-term-newborn-infants-and-adults-in-contrast-to-il-8
#16
Maria E Stålhammar, Richard Sindelar, Lena Douhan Håkansson
We have previously observed that neutrophils from neonates exhibit different migratory responses to intermediate and end-target chemoattractants compared to adults. The aim of the present study was to investigate the effect of the chemoattractants IL-8 (intermediate) and formyl-methionine-leucyl-phenylalanine (fMLP; end-target) on cell surface receptor expression involved in adhesion, migration and granule release of neutrophils from term newborn infants and adults. Heparinized cord blood from 16 healthy term infants delivered by caesarean section and peripheral blood from 17 healthy adults were incubated with 1 μM IL-8 or 0...
September 8, 2016: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/27595148/cd18-promoter-methylation-is-associated-with-a-higher-risk-of-thrombotic-complications-in-primary-myelofibrosis
#17
Claudia Augello, Daniele Cattaneo, Cristina Bucelli, Andrea Terrasi, Elisa Fermo, Ida Martinelli, Alessandra Iurlo, Umberto Gianelli
: Morbidity and mortality of BCR-ABL1-negative myeloproliferative neoplasm (MPN) patients are influenced by disease-related hemostatic complications, mostly of thrombotic nature. The pathogenesis of thrombosis is multifactorial: in particular, it has been demonstrated that a deregulated expression of Mac1 (also known as surface receptor integrin CD18/CD11b) by leukocytes has a role in favoring platelets' activation in MPN patients. Based on these data, we investigated the epigenetic status of CD18/CD11b in 78 primary myelofibrosis (PMF) patients to explore any possible association between the epigenetic profiles of these two genes and thrombotic risk...
December 2016: Annals of Hematology
https://www.readbyqxmd.com/read/27569996/the-pattern-of-gpi-80-expression-is-a-useful-marker-for-unusual-myeloid-maturation-in-peripheral-blood
#18
Y Takeda, T Kato, H Ito, Y Kurota, A Yamagishi, T Sakurai, A Araki, H Nara, N Tsuchiya, H Asao
Myeloid-derived suppressor cells (MDSCs) have a wide spectrum of immunosuppressive activity; control of these cells is a new target for improving clinical outcomes in cancer patients. MDSCs originate from unusual differentiation of neutrophils or monocytes induced by inflammatory cytokines, including granulocyte-colony stimulating factor (G-CSF) and granulocyte-macrophage (GM)-CSF. However, MDSCs are difficult to detect in neutrophil or monocyte populations because they are not uniform cells, resembling both neutrophils and monocytes; thus, they exist in a heterogeneous population...
December 2016: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/27539881/the-use-of-the-soluble-adhesion-molecules-se-selectin-sicam-1-svcam-1-specam-1-and-their-ligands-cd11a-and-cd49d-as-diagnostic-and-prognostic-biomarkers-in-septic-and-critically-ill-non-septic-icu-patients
#19
Anders G Kjaergaard, Anders Dige, Jeppe S Nielsen, Else Tønnesen, Jan Krog
Endothelial activation is pivotal in the development and escalation of sepsis. Central to endothelial activation is the endothelial up-regulation of cellular adhesion molecules (CAMs) including E-selectin, ICAM-1, VCAM-1, and PECAM-1. Shed CAMs are also found in circulating soluble forms (sCAMs). We investigated whether sCAMs can be used as biomarkers for the differentiation between septic and non-septic patients. Furthermore, we investigated lymphocyte and monocyte expression of LFA-1 (CD11a/CD18) and VLA-4 (CD49d/CD29) ligands for ICAM-1 and VCAM-1, respectively...
October 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27534550/folate-receptor-%C3%AE-regulates-integrin-cd11b-cd18-adhesion-of-a-macrophage-subset-to-collagen
#20
Christian Machacek, Verena Supper, Vladimir Leksa, Goran Mitulovic, Andreas Spittler, Karel Drbal, Miloslav Suchanek, Anna Ohradanova-Repic, Hannes Stockinger
Folate, also known as vitamin B9, is necessary for essential cellular functions such as DNA synthesis, repair, and methylation. It is supplied to the cell via several transporters and receptors, including folate receptor (FR) β, a GPI-anchored protein belonging to the folate receptor family. As FRβ shows a restricted expression to cells of myeloid origin and only a subset of activated macrophages and placental cells have been shown to express functional FRβ, it represents a promising target for future therapeutic strategies...
September 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
keyword
keyword
95812
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"