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https://www.readbyqxmd.com/read/28735409/silencing-of-stress-regulated-mirnas-in-plants-by-short-tandem-target-mimic-sttm-approach
#1
Sachin Teotia, Guiliang Tang
In plants, microRNAs (miRNAs) regulate more than hundred target genes comprising largely transcription factors that control growth and development as well as stress responses. However, the exact functions of miRNA families could not be deciphered because each miRNA family has multiple loci in the genome, thus are functionally redundant. Therefore, an ideal approach to study the function of a miRNA family is to silence the expression of all members simultaneously, which is a daunting task. However, this can be partly overcome by Target Mimic (TM) approach that can knockdown an entire miRNA family...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735408/rapid-quantification-of-abscisic-acid-by-gc-ms-ms-for-studies-of-abiotic-stress-response
#2
Paul E Verslues
Drought and low water potential induce large increases in Abscisic Acid (ABA ) content of plant tissue. This increased ABA content is essential to regulate downstream stress resistance responses; however, the mechanisms regulating ABA accumulation are incompletely known. Thus, the ability to accurately quantify ABA at high throughput and low cost is important for plant stress research. We have combined and modified several previously published protocols to establish a rapid ABA analysis protocol using gas chromatography-tandem mass spectrometry (GC-MS/MS)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735405/profiling-abscisic-acid-induced-changes-in-fatty-acid-composition-in-mosses
#3
Suhas Shinde, Shivakumar Devaiah, Aruna Kilaru
In plants, change in lipid composition is a common response to various abiotic stresses. Lipid constituents of bryophytes are of particular interest as they differ from that of flowering plants. Unlike higher plants, mosses have high content of very long-chain polyunsaturated fatty acids. Such lipids are considered to be important for survival of nonvascular plants. Here, using abscisic acid (ABA )-induced changes in lipid composition in Physcomitrella patens as an example, a protocol for total lipid extraction and quantification by gas chromatography (GC) coupled with flame ionization detector (FID) is described...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735402/determination-of-total-sulfur-sulfate-sulfite-thiosulfate-and-sulfolipids-in-plants
#4
Assylay Kurmanbayeva, Galina Brychkova, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, Moshe Sagi
In response to oxidative stress the biosynthesis of the ROS scavenger, glutathione is induced. This requires the induction of the sulfate reduction pathway for an adequate supply of cysteine, the precursor for glutathione. Cysteine also acts as the sulfur donor for the sulfuration of the molybdenum cofactor, crucial for the last step of ABA biosynthesis. Sulfate and sulfite are, respectively, the precursor and intermediate for cysteine biosynthesis and there is evidence for stress-induced sulfate uptake and further downstream, enhanced sulfite generation by 5'-phosphosulfate (APS) reductase (APR, EC 1...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735401/determination-of-enzymes-associated-with-sulfite-toxicity-in-plants-kinetic-assays-for-so-apr-sir-and-in-gel-sir-activity
#5
Galina Brychkova, Assylay Kurmanbayeva, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, Moshe Sagi
The amino acid cysteine plays a major role in plant response to abiotic stress by being the donor of elemental sulfur for the sulfuration of the molybdenum cofactor, otherwise the last step of ABA biosynthesis, the oxidation of abscisic aldehyde, is inactivated. Additionally, cysteine serves as a precursor for the biosynthesis of glutathione, the reactive oxygen species scavenger essential for redox status homeostasis during stress. Cysteine is generated by the sulfate reductive pathway where sulfite oxidase (SO; EC 1...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735388/epigenetics-and-rna-processing-connections-to-drought-salt-and-aba
#6
Min May Wong, Geeng Loo Chong, Paul E Verslues
There have been great research advances in epigenetics, RNA splicing, and mRNA processing over recent years. In parallel, there have been many advances in abiotic stress and Abscisic Acid (ABA) signaling. Here we overview studies that have examined stress-induced changes in the epigenome and RNA processing as well as cases where disrupting these processes changes the plant response to abiotic stress. We also highlight some examples where specific connections of stress or ABA signaling to epigenetics or RNA processing have been found...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28730861/comparative-outcomes-in-children-and-adults-with-anti-n-methyl-d-aspartate-anti-nmda-receptor-encephalitis
#7
Eliza Gordon-Lipkin, Anusha K Yeshokumar, Deanna Saylor, Ana Arenivas, John C Probasco
This study compared neurologic disability and adaptive function in children and adults >1 year following anti- N-methyl-d-aspartate receptor (anti-NMDAR) encephalitis diagnosis. Retrospective record review identified 12 patients with anti-NMDAR encephalitis. At last follow-up, all surviving patients had "good" modified Rankin Score (0-2). Four children, 6 adults, and their families participated in a telephone interview. Median duration since diagnosis was similar for children (2.42 years, interquartile range 2...
January 1, 2017: Journal of Child Neurology
https://www.readbyqxmd.com/read/28726973/heart-rate-variability-biofeedback-stress-relief-program-for-depression-a-replicated-single-subject-design
#8
Bregje M A Hartogs, Agna Bartels-Velthuis, Karen Van der Ploeg, Elske H Bos
BACKGROUND: Depressive disorders often have a chronic course and the efficacy of evidence-based treatments may be overestimated. OBJECTIVE: To examine the effectiveness of the Heart Rate Variability Stress Reduction Program (SRP) as a supplement to standard treatment in patients with depressive disorders. METHODS: The SRP was individually administered in eight weekly sessions. Seven participants completed the full protocol and were enrolled in a single-subject ABA multiple baseline experimental design...
July 19, 2017: Methods of Information in Medicine
https://www.readbyqxmd.com/read/28726715/overexpression-of-the-osimp-gene-increases-the-accumulation-of-inositol-and-confers-enhanced-cold-tolerance-in-tobacco-through-modulation-of-the-antioxidant-enzymes-activities
#9
Rong-Xiang Zhang, Li-Jun Qin, De-Gang Zhao
Inositol is a cyclic polyol that is involved in various physiological processes, including signal transduction and stress adaptation in plants. l-myo-inositol monophosphatase (IMPase) is one of the metal-dependent phosphatase family members and catalyzes the last reaction step of biosynthesis of inositol. Although increased IMPase activity induced by abiotic stress has been reported in chickpea plants, the role and regulation of the IMP gene in rice (Oryza sativa L.) remains poorly understood. In the present work, we obtained a full-length cDNA sequence coding IMPase in the cold tolerant rice landraces in Gaogonggui, which is named as OsIMP...
July 20, 2017: Genes
https://www.readbyqxmd.com/read/28725229/the-synergistic-priming-effect-of-exogenous-salicylic-acid-and-h2o2-on-chilling-tolerance-enhancement-during-maize-zea-mays-l-seed-germination
#10
Zhan Li, Jungui Xu, Yue Gao, Chun Wang, Genyuan Guo, Ying Luo, Yutao Huang, Weimin Hu, Mohamed S Sheteiwy, Yajing Guan, Jin Hu
Chilling stress is an important constraint for maize seedling establishment in the field. To examine the role of salicylic acid (SA) and hydrogen peroxide (H2O2) in response to chilling stress, we investigated the effects of seed priming with SA, H2O2, and SA+H2O2 combination on maize resistance under chilling stress (13°C). Priming with SA, H2O2, and especially SA+H2O2 shortened seed germination time and enhanced seed vigor and seedling growth as compared with hydropriming and non-priming treatments under low temperature...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28722263/ftsad2-and-ftjaz1-regulate-activity-of-the-ftmyb11-transcription-repressor-of-the-phenylpropanoid-pathway-in-fagopyrum-tataricum
#11
Meiliang Zhou, Zhanmin Sun, Mengqi Ding, Maria D Logacheva, Ivan Kreft, Dan Wang, Mingli Yan, Jirong Shao, Yixiong Tang, Yanmin Wu, Xuemei Zhu
Little is known about the molecular mechanism of the R2R3-MYB transcriptional repressors involved in plant phenylpropanoid metabolism. Here, we describe one R2R3-type MYB repressor, FtMYB11 from Fagopyrum tataricum. It contains the SID-like motif GGDFNFDL and it is regulated by both the importin protein 'Sensitive to ABA and Drought 2' (SAD2) and the jasmonates signalling cascade repressor JAZ protein. Yeast two hybrid and bimolecular fluorescence complementation assays demonstrated that FtMYB11 interacts with SAD2 and FtJAZ1...
July 19, 2017: New Phytologist
https://www.readbyqxmd.com/read/28720234/effect-of-music-therapy-on-the-anxiety-levels-and-pregnancy-rate-of-women-undergoing-in-vitro-fertilization-embryo-transfer-a-randomized-controlled-trial
#12
Yilda Arzu Aba, Dilek Avci, Yilmaz Guzel, Semanur Kumral Ozcelik, Basak Gurtekin
PURPOSE: The aim of this study was to determine the effect of music therapy on the anxiety levels and pregnancy rates of women who underwent in vitro fertilization-embryo transfer. METHODS: This prospective randomized controlled trial was conducted with 186 infertile women who presented to the In Vitro Fertilization Unit at the American Hospital in Turkey between April 2015 and April 2016. The infertile women who met the inclusion criteria were assigned to the music therapy group or the standard therapy group through block randomization...
August 2017: Applied Nursing Research: ANR
https://www.readbyqxmd.com/read/28719628/salt-tolerant-and-sensitive-alfalfa-medicago-sativa-cultivars-have-large-variations-in-defense-responses-to-the-lepidopteran-insect-spodoptera-litura-under-normal-and-salt-stress-condition
#13
Yunting Lei, Qing Liu, Christian Hettenhausen, Guoyan Cao, Qing Tan, Weiye Zhao, Honghui Lin, Jianqiang Wu
In nature, plants are often exposed to multiple stress factors at the same time. Yet, little is known about how plants modulate their physiology to counteract simultaneous abiotic and biotic stresses, such as soil salinity and insect herbivory. In this study, insect performance bioassays, phytohormone measurements, quantification of transcripts, and protein determination were employed to study the phenotypic variations of two alfalfa (Medicago sativa) cultivars in response to insect Spodoptera litura feeding under normal and salt stress condition...
2017: PloS One
https://www.readbyqxmd.com/read/28718648/multi-imaging-of-cytokinin-and-abscisic-acid-on-the-roots-of-rice-oryza-sativa-using-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#14
Katsuhiro Shiono, Riho Hashizaki, Toyofumi Nakanishi, Tatsuko Sakai, Takushi Yamamoto, Koretsugu Ogata, Ken-Ichi Harada, Hajime Ohtani, Hajime Katano, Shu Taira
Plant hormones are important mobile endogenous signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Visual information on more than one plant hormone from the same sample would be especially powerful, because it could enable rapid, intuitive interpretation. However, multi-imaging techniques for plant hormones have been unavailable. Here, we report the first multi-imaging of two plant hormones, one of cytokinin (i.e. trans-zeatin) and abscisic acid (ABA) by MALDI-TOF-MS imaging...
July 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28717112/effect-of-a-single-point-mutation-on-equine-herpes-virus-9-ehv-9-neuropathogenicity-after-intranasal-inoculation-in-a-hamster-model
#15
Asmaa G Saleh, Shehata I Anwar, Osama M Abas, Hoda A Abd-Ellatieff, Mohamed Nasr, Ibrahim Saleh, Hideto Fukushi, Tokuma Yanai
This study aimed to investigate the neuropathogenesis of equine herpes virus 9 (EHV-9) by studying the effects of a single point mutation introduced in two different EHV-9 genes. The two EHV-9 mutants, 14R and 19R, were generated carrying a point mutation in two separate EHV-9 genes. These mutants, along with the wild-type EHV-9, were used to infect a hamster model. The EHV-9- and 19R-infected groups showed earlier and more severe clinical signs of infection than the 14R-infected group. The white blood cells (WBCs) count was significantly increased in both EHV-9- and 19R-infected groups compared to the 14R-infected group at the 4th day post infection (DPI)...
July 15, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28716423/nadk2-positively-modulates-abscisic-acid-induced-stomatal-closure-by-affecting-accumulation-of-h2o2-ca-2-and-nitric-oxide-in-arabidopsis-guard-cells
#16
Lirong Sun, Yaping Li, Wenwen Miao, Tingting Piao, Yang Hao, Fu-Shun Hao
NAD kinase2 (NADK2) plays key roles in chloroplastic NADP biosynthesis, stress adaptation and modulation of cellular metabolisms in Arabidopsis. However, it is unknown whether and how NADK2 affects abscisic acid (ABA)-mediated stomatal movement. Here, we detected that null mutant nadk2 was more sensitive to drought stress than WT, and NADK2 gene was active in guard cells. Furthermore, NADK2 mutation impaired ABA-induced stomatal closure and ABA inhibition of light-promoted stomatal opening. NADK2 disruption also impaired ABA-stimulated accumulation of H2O2, Ca(2+) and nitric oxide (NO) in guard cells, but did not affect the stomatal closure evoked by exogenous H2O2, Ca(2+) or NO...
September 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28716419/a-%C3%AE-carotene-desaturase-gene-ibzds-increases-%C3%AE-carotene-and-lutein-contents-and-enhances-salt-tolerance-in-transgenic-sweetpotato
#17
Ruijie Li, Chen Kang, Xuejin Song, Ling Yu, Degao Liu, Shaozhen He, Hong Zhai, Qingchang Liu
ζ-Carotene desaturase (ZDS) is one of the key enzymes in carotenoid biosynthesis pathway. However, the ZDS gene has not been applied to carotenoid improvement of plants. Its roles in tolerance to abiotic stresses have not been reported. In this study, the IbZDS gene was isolated from storage roots of sweetpotato (Ipomoea batatas (L.) Lam.) cv. Nongdafu 14. Its overexpression significantly increased β-carotene and lutein contents and enhanced salt tolerance in transgenic sweetpotato (cv. Kokei No. 14) plants...
September 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28716417/alcohol-dehydrogenase-1-adh1-confers-both-abiotic-and-biotic-stress-resistance-in-arabidopsis
#18
Haitao Shi, Wen Liu, Yue Yao, Yunxie Wei, Zhulong Chan
Although the transcriptional regulation and upstream transcription factors of AtADH1 in response to abiotic stress are widely revealed, the in vivo roles of AtADH1 remain unknown. In this study, we found that the expression of AtADH1 was largely induced after salt, drought, cold and pathogen infection. Further studies found that AtADH1 overexpressing plants were more sensitive to abscisic acid (ABA) in comparison to wide type (WT), while AtADH1 knockout mutants showed no significant difference compared with WT in ABA sensitivity...
September 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28716048/transcriptome-analysis-uncovers-arabidopsis-f-box-stress-induced-1-as-a-regulator-of-jasmonic-acid-and-abscisic-acid-stress-gene-expression
#19
Lauren E Gonzalez, Kristen Keller, Karen X Chan, Megan M Gessel, Bryan C Thines
BACKGROUND: The ubiquitin 26S proteasome system (UPS) selectively degrades cellular proteins, which results in physiological changes to eukaryotic cells. F-box proteins are substrate adaptors within the UPS and are responsible for the diversity of potential protein targets. Plant genomes are enriched in F-box genes, but the vast majority of these have unknown roles. This work investigated the Arabidopsis F-box gene F-BOX STRESS INDUCED 1 (FBS1) for its effects on gene expression in order elucidate its previously unknown biological function...
July 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28714726/pilot-investigation-of-a-phosphatidylethanol-based-contingency-management-intervention-targeting-alcohol-use
#20
Michael G McDonell, Jordan Skalisky, Emily Leickly, Michael F Orr, Sterling McPherson, John Roll, Nathalie Hill-Kapturczak, Martin Javors
Phosphatidylethanol (PEth) can be detected in blood from 14 to as many as 28 days after alcohol consumption, depending on the amount and frequency of alcohol consumed. PEth may have utility for verifying abstinence in a contingency management (CM) intervention for alcohol use, particularly in settings where frequent verification of abstinence is impossible or impractical. Five nontreatment-seeking heavy drinkers (40% men) participated in an 11-week, ABA-phased within-subject experiment for which they submitted blood spots for PEth measurement, urine samples for ethyl glucuronide (EtG) testing, and self-report drinking data weekly...
July 17, 2017: Psychology of Addictive Behaviors: Journal of the Society of Psychologists in Addictive Behaviors
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