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https://www.readbyqxmd.com/read/28098233/microrna-34-directly-targets-pair-rule-genes-and-cytoskeleton-component-in-the-honey-bee
#1
Flávia C P Freitas, Camilla V Pires, Charles Claudianos, Alexandre S Cristino, Zilá L P Simões
MicroRNAs (miRNAs) are key regulators of developmental processes, such as cell fate determination and differentiation. Previous studies showed Dicer knockdown in honeybee embryos disrupt the processing of functional mature miRNAs and impairs embryo patterning. Here we investigated the expression profiles of miRNAs in honeybee embryogenesis and the role of the highly conserved miR-34-5p in the regulation of genes involved in insect segmentation. A total of 221 miRNAs were expressed in honey bee embryogenesis among which 97 mature miRNA sequences have not been observed before...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/27984144/lectin-from-seeds-of-a-brazilian-lima-bean-variety-phaseolus-lunatus-l-var-cascavel-presents-antioxidant-antitumour-and-gastroprotective-activities
#2
Rodrigo Rodrigues E Lacerda, Edilza Silva do Nascimento, José Thalles Jocelino Gomes de Lacerda, Luciano da Silva Pinto, Caroline Rizzi, Mirna Marques Bezerra, Isabela Ribeiro Pinto, Samuel Mateus Pereira Filho, Vicente de Paulo Texeira Pinto, Gerardo Cristino Filho, Carlos Alberto de Almeida Gadelha, Tatiane Santi Gadelha
Lectins are proteins able to interact specifically and reversibly with carbohydrates. They are present in all living beings, particularly in legume seeds, which have many biological functions. The aim of this study was to isolate, characterize and verify antioxidant, anti-hemolytic, antitumor and gastroprotective activities in a lectin present in seeds of Phaseolus lunatus L. var. cascavel (PLUN). The isolation of lectin was performed by size exclusion chromatography on Sephadex G-100, which was isolated from a protein capable of agglutinating only human erythrocytes type A, being this the only inhibited haemagglutination n-acetyl-d-galactosamine...
February 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27970496/parametric-simulation-of-headache-day-frequency-using-a-negative-binomial-distribution-a-case-study-of-erenumab-in-episodic-migraine
#3
J K Porter, A Brennan, S Palmer, S Sapra, J Cristino
No abstract text is available yet for this article.
November 2016: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27960021/-dyslipidemia-in-kidney-transplant-recipients
#4
Giovanni Mosconi, Camilla Gambaretto, Loretta Zambianchi, Maria Francesca Lifrieri, Marco De Fabritiis, Stefania Cristino, Claudio Americo, Maria Laura Angelini
The kidney transplant recipients' population shows pronounced alterations of the lipidic profile, with hypercholesterolemia (total cholesterol, LDL, VLDL), normal HDL and hypertriglyceridemia. Multiple factors contribute to the development of dyslipidemia, towards these, immunosuppressive therapy plays an important role. The impact on cardiovascular outcomes is less well defined than in general population. This work is a revaluation of the clinical approach to dyslipidemia in kidney transplant based on the more recent Guide Lines and literature...
2016: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/27920610/volumetric-image-guided-highly-conformal-radiotherapy-of-the-prostate-bed-toxicity-analysis
#5
Gianluca Ingrosso, Alessandra Carosi, Daniela di Cristino, Elisabetta Ponti, Andrea Lancia, Alessandra Murgia, Claudia Bruni, Pasquale Morelli, Franca Pietrasanta, Riccardo Santoni
AIM: To evaluate toxicity of high conformal image-guided radiotherapy of the prostate bed. BACKGROUND: Radiotherapy of the prostate bed has a pivotal role in the post-operative and salvage settings, but few clinical data are available on the use of daily image guidance in combination with highly conformal techniques, and data on long-term results are lacking. MATERIALS AND METHODS: We analyzed 118 patients irradiated on the prostate bed using conformal plans processed with a micro-multileaf collimator, and daily checking treatment set-up with a cone-beam CT system...
January 2017: Reports of Practical Oncology and Radiotherapy
https://www.readbyqxmd.com/read/27883274/impact-of-the-chronic-omega-3-fatty-acids-supplementation-in-hemiparkinsonism-model-induced-by-6-hydroxydopamine-in-rats
#6
Alexandre Sales Barros, Rafael Yuri Gouveia Crispim, Juliana Cavalcante Uchoa, Ricardo Basto Souza, Jonatas Cavalcante Lemos, Gerardo Cristino Filho, Mirna Marques Bezerra, Thales Fontenele Moraes Pinheiro, Silvânia Maria Mendes de Vasconcelos, Danielle Silveira Macêdo, Glauce Socorro de Barros Viana, Lissiana Magna Vasconcelos Aguiar
Parkinson's disease (PD) is characterized by a progressive degeneration of dopaminergic neurons in the substantia nigra. The neuronal degeneration may result from the convergence of a number of different pathogenic factors, including apoptosis, excitotoxicity and oxidative stress. Many studies emphasize the importance of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) in vital processes such as maintenance of the properties of cell membranes and the participation in signal transduction and biodynamic activity of neuronal membranes...
November 24, 2016: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/27790208/serotype-3-remains-the-leading-cause-of-invasive-pneumococcal-disease-in-adults-in-portugal-2012-2014-despite-continued-reductions-in-other-13-valent-conjugate-vaccine-serotypes
#7
Andreia N Horácio, Catarina Silva-Costa, Joana P Lopes, Mário Ramirez, José Melo-Cristino
Since 2010 the 13-valent pneumococcal conjugate vaccine (PCV13) replaced the 7-valent vaccine (PCV7) as the leading pneumococcal vaccine used in children through the private sector. Although, neither of the PCVs were used significantly in adults, changes in adult invasive pneumococcal disease (IPD) were expected due to herd protection. We characterized n = 1163 isolates recovered from IPD in adults in 2012-2014 with the goal of documenting possible changes in serotype prevalence and antimicrobial resistance...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27782497/biomimetic-coatings-enhance-tribocorrosion-behavior-and-cell-responses-of-commercially-pure-titanium-surfaces
#8
Isabella da Silva Vieira Marques, Maria Fernanda Alfaro, Miki Taketomi Saito, Nilson Cristino da Cruz, Christos Takoudis, Richard Landers, Marcelo Ferraz Mesquita, Francisco Humberto Nociti Junior, Mathew T Mathew, Cortino Sukotjo, Valentim Adelino Ricardo Barão
Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stability under wear and corrosion, and (3) to evaluate human mesenchymal stem cells (hMSCs) responses cultured on the modified surfaces. Tribocorrosion and cell experiments were performed following the MAO treatment...
August 11, 2016: Biointerphases
https://www.readbyqxmd.com/read/27747704/achieving-surface-chemical-and-morphologic-alterations-on-tantalum-by-plasma-electrolytic-oxidation
#9
Marcelo Augusto Pinto Cardoso Goularte, Gustavo Frainer Barbosa, Nilson Cristino da Cruz, Luciana Mayumi Hirakata
BACKGROUND: Search for materials that may either replace titanium dental implants or constitute an alternative as a new dental implant material has been widely studied. As well, the search for optimum biocompatible metal surfaces remains crucial. So, the aim of this work is to develop an oxidized surface layer on tantalum using plasma electrolytic oxidation (PEO) similar to those existing on oral implants been marketed today. METHODS: Cleaned tantalum samples were divided into group 1 (control) and groups 2, 3, and 4 (treated by PEO for 1, 3, and 5 min, respectively)...
December 2016: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/27612795/in-vitro-analysis-of-different-properties-of-acrylic-resins-for-ocular-prosthesis-submitted-to-accelerated-aging-with-or-without-photopolymerized-glaze
#10
Daniela Micheline Dos Santos, Bruna Egumi Nagay, Emily Vivianne Freitas da Silva, Liliane da Rocha Bonatto, Mariana Vilela Sonego, Amália Moreno, Elidiane Cipriano Rangel, Nilson Cristino da Cruz, Marcelo Coelho Goiato
The effect of a photopolymerized glaze on different properties of acrylic resin (AR) for ocular prostheses submitted to accelerated aging was investigated. Forty discs were divided into 4 groups: N1 AR without glaze (G1); colorless AR without glaze (G2); N1 AR with glaze (G3); and colorless AR with glaze (G4). All samples were polished with sandpaper (240, 600 and 800-grit). In G1 and G2, a 1200-grit sandpaper was also used. In G3 and G4, samples were coated with MegaSeal glaze. Property analysis of color stability, microhardness, roughness, and surface energy, and assays of atomic force microscopy, scanning electron microscopy, and energy-dispersive spectroscopy were performed before and after the accelerated aging (1008h)...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27612165/neuroprotective-effects-of-sulphated-agaran-from-marine-alga-gracilaria-cornea-in-rat-6-hydroxydopamine-parkinson-s-disease-model-behavioural-neurochemical-and-transcriptional-alterations
#11
Ricardo Basto Souza, Annyta Fernandes Frota, Rayane Siqueira Sousa, Nayara Araújo Cezario, Tarcizio Brito Santos, Luziana Mara Frota Souza, Chistiane Oliveira Coura, Valdécio Silvano Monteiro, Gerardo Cristino Filho, Silvânia Maria Mendes Vasconcelos, Rodrigo Maranguape Silva da Cunha, Lissiana Magna Vasconcelos Aguiar, Norma Maria Barros Benevides
Parkinson's disease (PD) is a multifactorial disease associated with the degeneration of dopaminergic neurons and behavioural alterations. Natural bioactive compounds may provide new therapeutic alternatives for neurodegenerative disorders, such as PD. The sulphated polysaccharides isolated from marine algae are heterogenic molecules that show different biological activities. The red marine alga Gracilaria cornea has a sulphated polysaccharide (SA-Gc) with structure and anti-inflammatory and antinociceptive activities reported in the literature...
September 9, 2016: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/27594667/capsule-impairs-efficient-adherence-of-streptococcus-agalactiae-to-intestinal-epithelium-in-tilapias-oreochromis-sp
#12
P Barato, E R Martins, G M Vasquez, M Ramirez, J Melo-Cristino, N Martínez, C Iregui
Streptococcosis caused by Streptococcus agalactiae is one of the most important diseases in the tilapia aquaculture industry. The role of the capsule of Streptococcus agalactiae in adherence to fish surfaces has not been evaluated and the mechanism of capsular regulation during adhesion has not been described. The aim of this study was to evaluate the role of the capsule of S. agalactiae during adhesion to intestinal epithelium of tilapia (Oreochromis sp.) in an ex vivo infection model. We show that the capsule impairs the adhesion of bacteria to host intestinal epithelium...
November 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27554205/technical-solutions-to-reduce-mediastinal-irradiation-in-young-patients-undergoing-treatment-for-lymphomas-preliminary-experience
#13
Michaela Benassi, Luana Di Murro, Barbara Tolu, Elisabetta Ponti, Maria Daniela Falco, Carla Rossi, Paolo Bagalà, Daniela di Cristino, Alessandra Murgia, Sara Cicchetti, Claudia Bruni, Pasquale Morelli, Andrea Lancia, Riccardo Santoni
This study aims at optimizing treatment planning in young patients affected by lymphoma (Stage II to III) by using an inclined board (IB) that allows reducing doses to the organs at risk. We evaluated 19 young patients affected by stage I to III lymphomas, referred to our Department for consolidation radiotherapy (RT) treatment on the mediastinum. Patients underwent 2 planning computed tomography (CT) scans performed in different positions: flat standard position and inclined position. A direct comparison between the different treatment plans was carried out analyzing dosimetric parameters obtained from dose-volume histograms generated for each plan...
January 2016: Medical Dosimetry: Official Journal of the American Association of Medical Dosimetrists
https://www.readbyqxmd.com/read/27549782/long-term-integrated-telerehabilitation-of-copd-patients-a-multicentre-randomised-controlled-trial-itrain
#14
Paolo Zanaboni, Birthe Dinesen, Audhild Hjalmarsen, Hanne Hoaas, Anne E Holland, Cristino Carneiro Oliveira, Richard Wootton
BACKGROUND: Pulmonary rehabilitation (PR) is an effective intervention for the management of people with chronic obstructive pulmonary disease (COPD). However, available resources are often limited, and many patients bear with poor availability of programmes. Sustaining PR benefits and regular exercise over the long term is difficult without any exercise maintenance strategy. In contrast to traditional centre-based PR programmes, telerehabilitation may promote more effective integration of exercise routines into daily life over the longer term and broaden its applicability and availability...
August 22, 2016: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/27530432/beta-hemolytic-streptococcus-dysgalactiae-strains-isolated-from-horses-are-a-genetically-distinct-population-within-the-streptococcus-dysgalactiae-taxon
#15
Marcos D Pinho, Erdal Erol, Bruno Ribeiro-Gonçalves, Catarina I Mendes, João A Carriço, Sandra C Matos, Silvia Preziuso, Antina Luebke-Becker, Lothar H Wieler, Jose Melo-Cristino, Mario Ramirez
The pathogenic role of beta-hemolytic Streptococcus dysgalactiae in the equine host is increasingly recognized. A collection of 108 Lancefield group C (n = 96) or L (n = 12) horse isolates recovered in the United States and in three European countries presented multilocus sequence typing (MLST) alleles, sequence types and emm types (only 56% of the isolates could be emm typed) that were, with few exceptions, distinct from those previously found in human Streptococcus dysgalactiae subsp. equisimilis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27524075/layer-by-layer-assembly-of-functionalized-reduced-graphene-oxide-for-direct-electrochemistry-and-glucose-detection
#16
Daniela Branco Tavares Mascagni, Celina Massumi Miyazaki, Nilson Cristino da Cruz, Marli Leite de Moraes, Antonio Riul, Marystela Ferreira
We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. The electron transport properties of graphene were combined with the specificity provided by the enzyme. rGO was obtained and functionalized using chemical methods, being positively charged with poly(diallyldimethylammonium chloride) to form GPDDA, and negatively charged with poly(styrene sulfonate) to form GPSS...
November 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27514370/biomimetic-coatings-enhance-tribocorrosion-behavior-and-cell-responses-of-commercially-pure-titanium-surfaces
#17
Isabella da Silva Vieira Marques, Maria Fernanda Alfaro, Miki Taketomi Saito, Nilson Cristino da Cruz, Christos Takoudis, Richard Landers, Marcelo Ferraz Mesquita, Francisco Humberto Nociti Junior, Mathew T Mathew, Cortino Sukotjo, Valentim Adelino Ricardo Barão
Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stability under wear and corrosion, and (3) to evaluate human mesenchymal stem cells (hMSCs) responses cultured on the modified surfaces. Tribocorrosion and cell experiments were performed following the MAO treatment...
2016: Biointerphases
https://www.readbyqxmd.com/read/27450241/immunochemical-localization-of-gabaa-receptor-subunits-in-the-freshwater-polyp-hydra-vulgaris-cnidaria-hydrozoa
#18
A Concas, R Imperatore, F Santoru, A Locci, P Porcu, L Cristino, P Pierobon
γ-aminobutyric acid (GABA) receptors, responding to GABA positive allosteric modulators, are present in the freshwater polyp Hydra vulgaris (Cnidaria, Hydrozoa), one of the most primitive metazoans to develop a nervous system. We examined the occurrence and distribution of GABAA receptor subunits in Hydra tissues by western blot and immunohistochemistry. Antibodies against different GABAA receptor subunits were used in Hydra membrane preparations. Unique protein bands, inhibited by the specific peptide, appeared at 35, 60, ∼50 and ∼52 kDa in membranes incubated with α3, β1, γ3 or δ antibodies, respectively...
November 2016: Neurochemical Research
https://www.readbyqxmd.com/read/27436148/formation-of-ox-1r-cb1r-heteromeric-complexes-in-embryonic-mouse-hypothalamic-cells-effect-on-intracellular-calcium-2-arachidonoyl-glycerol-biosynthesis-and-erk-phosphorylation
#19
Roberta Imperatore, Letizia Palomba, Giovanna Morello, Alessandro Di Spiezio, Fabiana Piscitelli, Vincenzo Di Marzo, Luigia Cristino
Orexin 1 (OX-1R) and cannabinoid receptor (CB1R) belong to the superfamily of G-protein-coupled receptors (GPCRs) and are mostly coupled to Gq and Gi/o proteins, respectively. In vitro studies in host cells over-expressing OX-1R and CB1R revealed a functional interaction between these receptors, through either their ability to form heteromers or the property for OX-1R to trigger the biosynthesis of 2-arachidonoylglycerol (2-AG), an endogenous CB1R ligand. Since: i) OX-1R and CB1R co-espression has been described at postsynaptc sites in hypothalamic circuits involved the regulation of energy homeostasis, and ii) increased orexin-A (OX-A) and 2-AG levels occur in hypothalamic neurons during obesity, we sought here to investigate the OX-1R/CB1R interaction in embryonic mouse hypothalamic NPY/AgRP mHypoE-N41 neurons which express, constitutively, both receptors...
September 2016: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/27396674/early-differential-sensitivity-of-evoked-potentials-to-local-and-global-shape-during-the-perception-of-three-dimensional-objects
#20
E Charles Leek, Mark Roberts, Zoe J Oliver, Filipe Cristino, Alan J Pegna
Here we investigated the time course underlying differential processing of local and global shape information during the perception of complex three-dimensional (3D) objects. Observers made shape matching judgments about pairs of sequentially presented multi-part novel objects. Event-related potentials (ERPs) were used to measure perceptual sensitivity to 3D shape differences in terms of local part structure and global shape configuration - based on predictions derived from hierarchical structural description models of object recognition...
August 2016: Neuropsychologia
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