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https://www.readbyqxmd.com/read/29325419/a-new-method-of-infrared-fluorescence-enhanced-thoracoscopic-segmentectomy
#1
Ahmed G Elkhouly, Benedetto Cristino, Eugenio Pompeo
Optimal identification of the intersegmental plane can be challenging during thoracoscopic anatomical segmentectomy for lung cancer. We describe a simple new method of infrared-fluorescence-enhanced thoracoscopy with selected injection of indocyanine green into the bronchi not targeted for resection, which allows us to clearly identify the intersegmental plane in thoracoscopic segmentectomy.
January 1, 2018: Asian Cardiovascular & Thoracic Annals
https://www.readbyqxmd.com/read/29289835/antinociceptive-anti-inflammatory-and-toxicological-evaluation-of-semi-synthetic-molecules-obtained-from-a-benzyl-isothiocyanate-isolated-from-moringa-oleifera-lam-in-a-temporomandibular-joint-inflammatory-hypernociception-model-in-rats
#2
Alain Oliveira Dos Santos, Danielle Rocha do Val, Felipe Dantas da Silveira, Francisco Isaac Fernandes Gomes, Hermany Capistrano Freitas, Ellen Lima de Assis, Diana Kelly Castro de Almeida, Igor Iuco Castro da Silva, Francisco Geraldo Barbosa, Jair Mafezoli, Marcos Reinaldo da Silva, Gerly Anne de Castro Brito, Juliana Trindade Clemente-Napimoga, Vicente de Paulo Teixeira de Paulo Teixera Pinto, Gerardo Cristino Filho, Mirna Marques Bezerra, Hellíada Vasconcelos Chaves
Inflammation is a key component of many clinical conditions that affect the temporomandibular joint (TMJ) and Moringa oleifera Lam. has been used to treat inflammatory diseases. Here, we evaluated the toxicological effects on mice of a naturally-occurring isothiocyanate from M. oleifera and its seven analogue molecules. Further, the anti-nociceptive and anti-inflammatory effects on a rat model of TMJ inflammatory hypernociception were assessed. The systemic toxicological profile was determined in mice over a 14-day period: MC-1 1 μg/kg; MC-D1 1 μg/kg, MC-D3 100 μg/kg, MC-D6 1 μg/kg, MC-D7 1 μg/kg, MC-D8 1 μg/kg, MC-D9 10 μg/kg, and MC-H 1 μg/kg...
December 28, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29249988/stemodia-maritima-l-extract-decreases-inflammation-oxidative-stress-and-alveolar-bone-loss-in-an-experimental-periodontitis-rat-model
#3
Alrieta H Teixeira, Jordânia M de Oliveira Freire, Luzia H T de Sousa, Antônia T Parente, Nayara A de Sousa, Angela M C Arriaga, Francisca R Lopes da Silva, Iracema M Melo, Igor I Castro da Silva, Karuza M A Pereira, Paula Goes, José J do Nascimento Costa, Gerardo Cristino-Filho, Vicente de Paulo T Pinto, Hellíada V Chaves, Mirna M Bezerra
Periodontitis is very prevalent worldwide and is one of the major causes of tooth loss in adults. About 80% of the worldwide population use medicinal plants for their health care. Stemodia maritima L. (S. maritima) antioxidant and antimicrobial effects in vitro as well as anti-inflammatory properties. Herein, the potential therapeutic effect of S. maritima was assessed in rats subjected to experimental periodontitis (EP). EP was induced in female Wistar rats by nylon thread ligature around 2nd upper left molars for 11 days...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29175669/euterpe-oleracea-mart-a%C3%A3-a%C3%A3-seed-extract-associated-with-exercise-training-reduces-hepatic-steatosis-in-type-2-diabetic-male-rats
#4
Graziele Freitas de Bem, Cristiane Aguiar da Costa, Viviane da Silva Cristino Cordeiro, Izabelle Barcellos Santos, Lenize Costa Reis Marins de Carvalho, Ricardo de Andrade Soares, Jéssica Honorato Ribeiro, Marcelo Augusto Vieira de Souza, Pergentino José da Cunha Sousa, Dayane Teixeira Ognibene, Angela Castro Resende, Roberto Soares de Moura
Type 2 diabetes mellitus contributes to an increased risk of metabolic and morphological changes in key organs, such as the liver. We aimed to assess the effect of the açaí seed extract (ASE) associated with exercise training on hepatic steatosis induced by high-fat (HF) diet plus streptozotocin (STZ) in rats. Type 2 diabetes was induced by feeding rats with HF diet (55% fat) for 5 weeks, followed by a single low dose of STZ (35 mg/kg i.p.). Control and diabetic groups were subdivided into four groups that were fed with standard chow diet for 4 weeks...
October 13, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29046296/geraniin-is-a-diuretic-by-inhibiting-the-na-k-2cl-cotransporter-nkcc2
#5
Erika Moreno, Juan A Gayosso, Jose Ramón Montejano, Georgina Almaguer, Norma Vázquez, Cristino Cruz, Adriana Mercado, Norma A Bobadilla, Gerardo Gamba, Alfredo Sierra, Victoria Ramírez
Geranium seemannii Peyr is a perennial plant endemic in central Mexico that has been widely used for its diuretic effect, but the responsible compound of this effect is unknown as well as the mechanism by which the diuretic effect is achieved. Geraniin is one of the compounds isolated from this kind of geranium. This study was designed to determinate whether geraniin possesses diuretic activity and to elucidate the mechanism of action. Geraniin was extracted and purified from Geranium seemannii Peyr. Male Wistar rats were divided into four groups: 1) Control, 2) 75 mg/kg of geraniin, 3) 20 mg/Kg of furosemide, and 4) 10 mg/Kg of hydrochlorothiazide...
October 18, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29023550/effects-of-acute-alcohol-ingestion-on-eye-movements-and-cognition-a-double-blind-placebo-controlled-study
#6
Jéssica Bruna Santana Silva, Eva Dias Cristino, Natalia Leandro de Almeida, Paloma Cavalcante Bezerra de Medeiros, Natanael Antonio Dos Santos
Alcohol is one of the most consumed psychoactive substances in the world, and the negative impact related to alcohol use has become a worldwide public health issue. Alcohol is able to affect diffusely several areas of the Central Nervous System, which could impair visual functions, including eye movements, and cognitive processes. The objective of the present study was to investigate the effects of moderate alcohol intake in eyes movements, as an indicator of cognitive processing underlying the visual search in a the Maze task...
2017: PloS One
https://www.readbyqxmd.com/read/29022728/stereo-viewing-modulates-three-dimensional-shape-processing-during-object-recognition-a-high-density-erp-study
#7
Zoe J Oliver, Filipe Cristino, Mark V Roberts, Alan J Pegna, E Charles Leek
The role of stereo disparity in the recognition of 3-dimensional (3D) object shape remains an unresolved issue for theoretical models of the human visual system. We examined this issue using high-density (128 channel) recordings of event-related potentials (ERPs). A recognition memory task was used in which observers were trained to recognize a subset of complex, multipart, 3D novel objects under conditions of either (bi-) monocular or stereo viewing. In a subsequent test phase they discriminated previously trained targets from untrained distractor objects that shared either local parts, 3D spatial configuration, or neither dimension, across both previously seen and novel viewpoints...
October 12, 2017: Journal of Experimental Psychology. Human Perception and Performance
https://www.readbyqxmd.com/read/28954435/industrial-cooling-tower-disinfection-treatment-to-prevent-legionella-spp
#8
Matteo Iervolino, Benedetta Mancini, Sandra Cristino
The contamination of industrial cooling towers has been identified as one cause of legionellosis, but the real risk has been underestimated. Two different disinfection treatments were tested on Legionella colonization in an industrial Cooling Tower System (CTS). Environmental monitoring of Legionella, P. aeruginosa, and a heterotrophic plate count (HPC) at 36 °C was performed from June to October 2016. The disinfection procedures adopted were based on hydrogen peroxide (H₂O₂) and silver salts (Ag⁺), in addition to an anti-algal treatment, then using hyperclorination as a shock, and then continuous treatment by sodium hypochlorite (NaClO)...
September 26, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28840618/the-beneficial-effect-of-anthocyanidin-rich-vitis-vinifera-l-grape-skin-extract-on-metabolic-changes-induced-by-high-fat-diet-in-mice-involves-antiinflammatory-and-antioxidant-actions
#9
Gisele França da Costa, Izabelle Barcellos Santos, Graziele Freitas de Bem, Viviane Silva Cristino Cordeiro, Cristiane Aguiar da Costa, Lenize Costa Reis Marins de Carvalho, Dayane Teixeira Ognibene, Angela Castro Resende, Roberto Soares de Moura
We hypothesized that a polyphenol-rich extract from Vitis vinifera L. grape skin (GSE) may exert beneficial effects on obesity and related metabolic disorders induced by a high-fat diet (HFD). C57/BL6 mice were fed a standard diet (10% fat, control, and GSE groups) or an HFD (60% fat, high fat (HF), and HF + GSE) with or without GSE (200 mg/kg/day) for 12 weeks. GSE prevented weight gain; dyslipidemia; insulin resistance; the alterations in plasma levels of leptin, adiponectin, and resistin; and the deregulation of leptin and adiponectin expression in adipose tissue...
August 25, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28820285/oligometastatic-cancer-stereotactic-ablative-radiotherapy-for-patients-affected-by-isolated-body-metastasis
#10
Andrea Lancia, Gianluca Ingrosso, Alessandra Carosi, Luana Di Murro, Emilia Giudice, Sara Cicchetti, Pasquale Morelli, Daniela di Cristino, Claudia Bruni, Alessandra Murgia, Alessandro Cancelli, Irene Turturici, Anjali Iadevaia, Elisabetta Ponti, Riccardo Santoni
BACKGROUND: To evaluate the outcome of patients affected by a single isolated body metastasis treated with stereotactic body radiotherapy (SBRT). MATERIAL AND METHODS: Seven-eight patients were treated with SBRT for isolated body metastasis. The most frequent primary tumor was prostate cancer (28.2%), followed by colorectal cancer (23.1%) and lung cancer (20.5%). Median age at diagnosis of oligometastatic disease was 70 years (range 47-88). Median Karnofsky Performance Status (KPS) was 90 (range 70-100)...
November 2017: Acta Oncologica
https://www.readbyqxmd.com/read/28750816/techniques-for-the-cellular-and-subcellular-localization-of-endocannabinoid-receptors-and-enzymes-in-the-mammalian-brain
#11
Luigia Cristino, Roberta Imperatore, Vincenzo Di Marzo
This chapter attempts to piece together knowledge about new advanced microscopy techniques to study the neuroanatomical distribution of endocannabinoid receptors and enzymes at the level of cellular and subcellular structures and organelles in the brain. Techniques ranging from light to electron microscopy up to the new advanced LBM, PALM, and STORM super-resolution microscopy will be discussed in the context of their contribution to define the spatial distribution and organization of receptors and enzymes of the endocannabinoid system (ECS), and to better understand ECS brain functions...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28745525/balance-and-falls-in-acute-exacerbation-of-chronic-obstructive-pulmonary-disease-a-prospective-study
#12
Cristino C Oliveira, Annemarie L Lee, Jennifer McGinley, Gary P Anderson, Ross A Clark, Michelle Thompson, Sandy Clarke, Tamara Baker, Louis B Irving, Linda Denehy
Individuals with chronic obstructive pulmonary disease (COPD) have demonstrated balance impairment and a higher fall incidence. However, these have not been investigated in acute exacerbations of the disease (ECOPD). This study evaluates balance in patients during an ECOPD compared to stable COPD and healthy controls, and examines the fall incidence rate after hospitalisation due to ECOPD compared to individuals with stable COPD. Balance performance of 26 hospitalised patients with ECOPD was compared to 26 community-dwelling participants with stable COPD and 25 matched healthy controls...
October 2017: COPD
https://www.readbyqxmd.com/read/28739056/supplementation-with-vitis-vinifera-l-skin-extract-improves-insulin-resistance-and-prevents-hepatic-lipid-accumulation-and-steatosis-in-high-fat-diet-fed-mice
#13
Izabelle Barcellos Santos, Graziele Freitas de Bem, Viviane Silva Cristino Cordeiro, Cristiane Aguiar da Costa, Lenize Costa Reis Marins de Carvalho, Ana Paula Machado da Rocha, Gisele França da Costa, Dayane Teixeira Ognibene, Roberto Soares de Moura, Angela Castro Resende
Nonalcoholic fatty liver disease is one of the most common complications of obesity. The Vitis vinifera L. grape skin extract (ACH09) is an important source of polyphenols, which are related to its antioxidant and antihyperglycemic activities. We hypothesized that ACH09 could also exert beneficial effects on metabolic disorders associated with obesity and evaluated ACH09's influence on high-fat (HF) diet-induced hepatic steatosis and insulin resistance in C57BL/6 mice. The animals were fed a standard diet (10% fat, control) or an HF diet (60% fat, HF) with or without ACH09 (200mg/[kg d]) for 12weeks...
July 2017: Nutrition Research
https://www.readbyqxmd.com/read/28689901/effect-of-nonthermal-plasma-on-the-properties-of-a-resinous-liner-submitted-to-aging
#14
Marcelo Coelho Goiato, Emily Vivianne Freitas da Silva, Rodrigo Antonio de Medeiros, Sandro Basso Bitencourt, Elidiane Cipriano Rangel, Nilson Cristino da Cruz, Daniela Micheline Dos Santos
STATEMENT OF PROBLEM: The properties, such as softness and viscoelasticity, of a resinous reliner can deteriorate and extrinsic elements can become incorporated, making surface protection of the reliner material essential. PURPOSE: The purpose of this in vitro study was to evaluate the effect of low temperature plasma on Coe-Soft resinous reliner, submitted to aging in artificial saliva for up to 180 days. Sorption, solubility, Shore A hardness, surface energy, and topographic characteristics were analyzed by scanning electronic microscopy (SEM) and energy-dispersive spectroscopy (EDS)...
July 7, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28669028/the-excitatory-inhibitory-input-to-orexin-hypocretin-neuron-soma-undergoes-day-night-reorganization
#15
Claudia Laperchia, Roberta Imperatore, Idris A Azeez, Federico Del Gallo, Giuseppe Bertini, Gigliola Grassi-Zucconi, Luigia Cristino, Marina Bentivoglio
Orexin (OX)/hypocretin-containing neurons are main regulators of wakefulness stability, arousal, and energy homeostasis. Their activity varies in relation to the animal's behavioral state. We here tested whether such variation is subserved by synaptic plasticity phenomena in basal conditions. Mice were sacrificed during day or night, at times when sleep or wake, respectively, predominates, as assessed by electroencephalography in matched mice. Triple immunofluorescence was used to visualize OX-A perikarya and varicosities containing the vesicular glutamate transporter (VGluT)2 or the vesicular GABA transporter (VGAT) combined with synaptophysin (Syn) as a presynaptic marker...
July 1, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/28650699/effect-of-euterpe-oleracea-mart-seeds-extract-on-chronic-ischemic-renal-injury-in-renovascular-hypertensive-rats
#16
Cristiane Aguiar da Costa, Dayane Teixeira Ognibene, Viviane Silva Cristino Cordeiro, Graziele Freitas de Bem, Izabelle Barcellos Santos, Ricardo Andrade Soares, Letícia L de Melo Cunha, Lenize C R M Carvalho, Roberto Soares de Moura, Angela Castro Resende
Previously, we have demonstrated that the seeds of Euterpe oleracia Mart. (açaí) are rich in polyphenols with antihypertensive and antioxidant properties. This study evaluated the renal protective effects of the hydroalcoholic extract obtained from the seeds of açaí (ASE) fruits in two-kidney, one-clip (2K1C) renovascular hypertension. Young male Wistar rats were used to obtain 2K1C and sham groups. Animals received ASE (200 mg/(kg·day) in drinking water) or vehicle for 40 days. We evaluated serum and urinary parameters, renal structural changes, and oxidative status...
October 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28587937/anti-inflammatory-and-anti-nociceptive-effects-of-strontium-ranelate-on-the-zymosan-induced-temporomandibular-joint-inflammatory-hypernociception-in-rats-depend-on-tnf-%C3%AE-inhibition
#17
Sheila Moreira Alves, Susana Capistrano Abreu, Jonas Cavalcante Lemos, Francisco Isaac Fernandes Gomes, Shirley Moreira Alves, Danielle Rocha do Val, Raul Sousa Freitas, Karuza Maria Alves Pereira, Vicente de Paulo Teixeira Pinto, Gerly Anne de Castro Brito, Mirna Marques Bezerra, Gerardo Cristino-Filho, Hellíada Vasconcelos Chaves
BACKGROUND: Temporomandibular joint (TMJ) disorders show inflammatory components, heavily impacting on quality of life. Strontium ranelate has previously shown anti-inflammatory and antinociceptive effects on other experimental inflammatory pain models. Thus, we aim to investigate the strontium ranelate efficacy in reducing the zymosan-induced inflammatory hypernociception in the TMJ of rats by evaluating the TNF-α, IL-1β, and hemeoxygenase-1 (HO-1) involvement. METHODS: Wistar rats were treated with strontium ranelate (0...
March 12, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28580289/endocannabinoid-dependent-disinhibition-of-orexinergic-neurons-electrophysiological-evidence-in-leptin-knockout-obese-mice
#18
Thorsten Michael Becker, Morgana Favero, Vincenzo Di Marzo, Luigia Cristino, Giuseppe Busetto
OBJECTIVES: In the ob/ob mouse model of obesity, chronic absence of leptin causes a significant increase of orexin (OX) production by hypothalamic neurons and excessive food intake. The altered OX level is linked to a dramatic increase of the inhibitory innervation of OX producing neurons (OX neurons) and the over expression of the endocannabinoid 2-arachidonoylglycerol (2-AG) by OX neurons of ob/ob mice. Little is known about the function of the excitatory synapses of OX neurons in ob/ob mice, and their modulation by 2-AG...
June 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/28579186/endocannabinoid-modulation-of-homeostatic-and-non-homeostatic-feeding-circuits
#19
REVIEW
Benjamin K Lau, Daniela Cota, Luigia Cristino, Stephanie L Borgland
The endocannabinoid system has emerged as a key player in the control of eating. Endocannabinoids, including 2-arachidonoylglycerol (2-AG) and anandamide (AEA), modulate neuronal activity via cannabinoid 1 receptors (CB1Rs) in multiple nuclei of the hypothalamus to induce or inhibit food intake depending on nutritional and hormonal status, suggesting that endocannabinoids may act in the hypothalamus to integrate different types of signals informing about the animal's energy needs. In the mesocorticolimbic system, (endo)cannabinoids modulate synaptic transmission to promote dopamine release in response to palatable food...
June 1, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28578413/dna-methylation-in-schizophrenia-in-different-patient-derived-cell-types
#20
Alejandra M Vitale, Nicholas A Matigian, Alexandre S Cristino, Katia Nones, Sugandha Ravishankar, Bernadette Bellette, Yongjun Fan, Stephen A Wood, Ernst Wolvetang, Alan Mackay-Sim
DNA methylation of gene promoter regions represses transcription and is a mechanism via which environmental risk factors could affect cells during development in individuals at risk for schizophrenia. We investigated DNA methylation in patient-derived cells that might shed light on early development in schizophrenia. Induced pluripotent stem cells may reflect a "ground state" upon which developmental and environmental influences would be minimal. Olfactory neurosphere-derived cells are an adult-derived neuro-ectodermal stem cell modified by developmental and environmental influences...
January 23, 2017: NPJ Schizophrenia
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