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Marcelo Augusto Pinto Cardoso Goularte, Gustavo Frainer Barbosa, Nilson Cristino da Cruz, Luciana Mayumi Hirakata
BACKGROUND: Search for materials that may either replace titanium dental implants or constitute an alternative as a new dental implant material has been widely studied. As well, the search for optimum biocompatible metal surfaces remains crucial. So, the aim of this work is to develop an oxidized surface layer on tantalum using plasma electrolytic oxidation (PEO) similar to those existing on oral implants been marketed today. METHODS: Cleaned tantalum samples were divided into group 1 (control) and groups 2, 3, and 4 (treated by PEO for 1, 3, and 5 min, respectively)...
December 2016: Int J Implant Dent
Daniela Micheline Dos Santos, Bruna Egumi Nagay, Emily Vivianne Freitas da Silva, Liliane da Rocha Bonatto, Mariana Vilela Sonego, Amália Moreno, Elidiane Cipriano Rangel, Nilson Cristino da Cruz, Marcelo Coelho Goiato
The effect of a photopolymerized glaze on different properties of acrylic resin (AR) for ocular prostheses submitted to accelerated aging was investigated. Forty discs were divided into 4 groups: N1 AR without glaze (G1); colorless AR without glaze (G2); N1 AR with glaze (G3); and colorless AR with glaze (G4). All samples were polished with sandpaper (240, 600 and 800-grit). In G1 and G2, a 1200-grit sandpaper was also used. In G3 and G4, samples were coated with MegaSeal glaze. Property analysis of color stability, microhardness, roughness, and surface energy, and assays of atomic force microscopy, scanning electron microscopy, and energy-dispersive spectroscopy were performed before and after the accelerated aging (1008h)...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Ricardo Basto Souza, Annyta Fernandes Frota, Rayane Siqueira Sousa, Nayara Araújo Cezario, Tarcizio Brito Santos, Luziana Mara Frota Souza, Chistiane Oliveira Coura, Valdécio Silvano Monteiro, Gerardo Cristino Filho, Silvânia Maria Mendes Vasconcelos, Rodrigo Maranguape Silva da Cunha, Lissiana Magna Vasconcelos Aguiar, Norma Maria Barros Benevides
Parkinson's disease (PD) is a multifactorial disease associated with the degeneration of dopaminergic neurons and behavioural alterations. Natural bioactive compounds may provide new therapeutic alternatives for neurodegenerative disorders, such as PD. The sulphated polysaccharides isolated from marine algae are heterogenic molecules that show different biological activities. The red marine alga Gracilaria cornea has a sulphated polysaccharide (SA-Gc) with structure and anti-inflammatory and antinociceptive activities reported in the literature...
September 9, 2016: Basic & Clinical Pharmacology & Toxicology
P Barato, E R Martins, G M Vasquez, M Ramirez, J Melo-Cristino, N Martínez, C Iregui
Streptococcosis caused by Streptococcus agalactiae is one of the most important diseases in the tilapia aquaculture industry. The role of the capsule of Streptococcus agalactiae in adherence to fish surfaces has not been evaluated and the mechanism of capsular regulation during adhesion has not been described. The aim of this study was to evaluate the role of the capsule of S. agalactiae during adhesion to intestinal epithelium of tilapia (Oreochromis sp.) in an ex vivo infection model. We show that the capsule impairs the adhesion of bacteria to host intestinal epithelium...
September 2, 2016: Microbial Pathogenesis
Michaela Benassi, Luana Di Murro, Barbara Tolu, Elisabetta Ponti, Maria Daniela Falco, Carla Rossi, Paolo Bagalà, Daniela di Cristino, Alessandra Murgia, Sara Cicchetti, Claudia Bruni, Pasquale Morelli, Andrea Lancia, Riccardo Santoni
This study aims at optimizing treatment planning in young patients affected by lymphoma (Stage II to III) by using an inclined board (IB) that allows reducing doses to the organs at risk. We evaluated 19 young patients affected by stage I to III lymphomas, referred to our Department for consolidation radiotherapy (RT) treatment on the mediastinum. Patients underwent 2 planning computed tomography (CT) scans performed in different positions: flat standard position and inclined position. A direct comparison between the different treatment plans was carried out analyzing dosimetric parameters obtained from dose-volume histograms generated for each plan...
August 20, 2016: Medical Dosimetry: Official Journal of the American Association of Medical Dosimetrists
Paolo Zanaboni, Birthe Dinesen, Audhild Hjalmarsen, Hanne Hoaas, Anne E Holland, Cristino Carneiro Oliveira, Richard Wootton
BACKGROUND: Pulmonary rehabilitation (PR) is an effective intervention for the management of people with chronic obstructive pulmonary disease (COPD). However, available resources are often limited, and many patients bear with poor availability of programmes. Sustaining PR benefits and regular exercise over the long term is difficult without any exercise maintenance strategy. In contrast to traditional centre-based PR programmes, telerehabilitation may promote more effective integration of exercise routines into daily life over the longer term and broaden its applicability and availability...
August 22, 2016: BMC Pulmonary Medicine
Marcos D Pinho, Erdal Erol, Bruno Ribeiro-Gonçalves, Catarina I Mendes, João A Carriço, Sandra C Matos, Silvia Preziuso, Antina Luebke-Becker, Lothar H Wieler, Jose Melo-Cristino, Mario Ramirez
The pathogenic role of beta-hemolytic Streptococcus dysgalactiae in the equine host is increasingly recognized. A collection of 108 Lancefield group C (n = 96) or L (n = 12) horse isolates recovered in the United States and in three European countries presented multilocus sequence typing (MLST) alleles, sequence types and emm types (only 56% of the isolates could be emm typed) that were, with few exceptions, distinct from those previously found in human Streptococcus dysgalactiae subsp. equisimilis...
2016: Scientific Reports
Daniela Branco Tavares Mascagni, Celina Massumi Miyazaki, Nilson Cristino da Cruz, Marli Leite de Moraes, Antonio Riul, Marystela Ferreira
We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. The electron transport properties of graphene were combined with the specificity provided by the enzyme. rGO was obtained and functionalized using chemical methods, being positively charged with poly(diallyldimethylammonium chloride) to form GPDDA, and negatively charged with poly(styrene sulfonate) to form GPSS...
November 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Isabella da Silva Vieira Marques, Maria Fernanda Alfaro, Miki Taketomi Saito, Nilson Cristino da Cruz, Christos Takoudis, Richard Landers, Marcelo Ferraz Mesquita, Francisco Humberto Nociti Junior, Mathew T Mathew, Cortino Sukotjo, Valentim Adelino Ricardo Barão
Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stability under wear and corrosion, and (3) to evaluate human mesenchymal stem cells (hMSCs) responses cultured on the modified surfaces. Tribocorrosion and cell experiments were performed following the MAO treatment...
2016: Biointerphases
A Concas, R Imperatore, F Santoru, A Locci, P Porcu, L Cristino, P Pierobon
γ-aminobutyric acid (GABA) receptors, responding to GABA positive allosteric modulators, are present in the freshwater polyp Hydra vulgaris (Cnidaria, Hydrozoa), one of the most primitive metazoans to develop a nervous system. We examined the occurrence and distribution of GABAA receptor subunits in Hydra tissues by western blot and immunohistochemistry. Antibodies against different GABAA receptor subunits were used in Hydra membrane preparations. Unique protein bands, inhibited by the specific peptide, appeared at 35, 60, ∼50 and ∼52 kDa in membranes incubated with α3, β1, γ3 or δ antibodies, respectively...
July 23, 2016: Neurochemical Research
Roberta Imperatore, Letizia Palomba, Giovanna Morello, Alessandro Di Spiezio, Fabiana Piscitelli, Vincenzo Di Marzo, Luigia Cristino
Orexin 1 (OX-1R) and cannabinoid receptor (CB1R) belong to the superfamily of G-protein-coupled receptors (GPCRs) and are mostly coupled to Gq and Gi/o proteins, respectively. In vitro studies in host cells over-expressing OX-1R and CB1R revealed a functional interaction between these receptors, through either their ability to form heteromers or the property for OX-1R to trigger the biosynthesis of 2-arachidonoylglycerol (2-AG), an endogenous CB1R ligand. Since: i) OX-1R and CB1R co-espression has been described at postsynaptc sites in hypothalamic circuits involved the regulation of energy homeostasis, and ii) increased orexin-A (OX-A) and 2-AG levels occur in hypothalamic neurons during obesity, we sought here to investigate the OX-1R/CB1R interaction in embryonic mouse hypothalamic NPY/AgRP mHypoE-N41 neurons which express, constitutively, both receptors...
September 2016: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
E Charles Leek, Mark Roberts, Zoe J Oliver, Filipe Cristino, Alan J Pegna
Here we investigated the time course underlying differential processing of local and global shape information during the perception of complex three-dimensional (3D) objects. Observers made shape matching judgments about pairs of sequentially presented multi-part novel objects. Event-related potentials (ERPs) were used to measure perceptual sensitivity to 3D shape differences in terms of local part structure and global shape configuration - based on predictions derived from hierarchical structural description models of object recognition...
August 2016: Neuropsychologia
Joana Campos, Luís Cristino, Luísa Peixe, Patrícia Antunes
The mcr-1 gene was found in 11 isolates from a Portuguese Salmonella collection (n = 1,010; 58 serotypes; 2002-15) of clinical samples, foodstuff, food-animals and water. Mcr-1 has been located on different plasmids (IncX4/IncHI2) in pig-associated multidrug-resistant, copper-tolerant S.1,4,[5],12:i:-/ST34 and S. Rissen/ST469 clones from human and pork products since at least 2011. Our data highlight dissemination of mcr-1 by successful resistant clones in Europe and raise questions about the efficacy of copper-based interventions to reduce colistin use...
June 30, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Anup D Shah, Kerry L Inder, Alok K Shah, Alexandre S Cristino, Arthur B McKie, Hani Gabra, Melissa Davis, Michelle M Hill
Lipid rafts are dynamic membrane micro-domains that orchestrate molecular interactions, and are implicated in cancer development. To understand the functions of lipid rafts in cancer we performed an integrated analysis of quantitative lipid raft proteomics datasets modeling progression in breast cancer, melanoma and renal cell carcinoma. This analysis revealed that cancer development is associated with increased membrane raft-cytoskeleton interactions, with around 40% of elevated lipid raft proteins being cytoskeletal components...
July 7, 2016: Journal of Proteome Research
Raul Sousa Freitas, Danielle Rocha do Val, Maria Ester Frota Fernandes, Francisco Isaac Fernandes Gomes, José Thalles Jocelino Gomes de Lacerda, Tatiane SantiGadelha, Carlos Alberto de Almeida Gadelha, Vicente de Paulo Teixeira Pinto, Gerardo Cristino-Filho, Karuza Maria Alves Pereira, Gerly Anne de Castro Brito, Mirna Marques Bezerra, Hellíada Vasconcelos Chaves
Temporomandibular joint (TMJ) disorders show inflammatory components, heavily impacting on quality of life. Abelmoschus esculentus is largely cultivated in Northeastern Brazil for medicinal purposes, having it shown anti-inflammatory activity. We evaluated A. esculentus lectin (AEL) efficacy in reducing zymosan-induced temporomandibular joint inflammatory hypernociception in rats along with the mechanism of action through which it exerts anti-inflammatory activity. Animals were pre-treated with AEL (0.01, 0...
September 2016: International Immunopharmacology
Carla Mottola, Carina S Matias, João J Mendes, José Melo-Cristino, Luís Tavares, Patrícia Cavaco-Silva, Manuela Oliveira
BACKGROUND: Foot infections are a major cause of morbidity in people with diabetes and the most common cause of diabetes-related hospitalization and lower extremity amputation. Staphylococcus aureus is by far the most frequent species isolated from these infections. In particular, methicillin-resistant S. aureus (MRSA) has emerged as a major clinical and epidemiological problem in hospitals. MRSA strains have the ability to be resistant to most β-lactam antibiotics, but also to a wide range of other antimicrobials, making infections difficult to manage and very costly to treat...
June 23, 2016: BMC Microbiology
Sofia Santos Costa, Cláudia Palma, Kristina Kadlec, Andrea T Fessler, Miguel Viveiros, José Melo-Cristino, Stefan Schwarz, Isabel Couto
Plasmids play a key role in the genetic plasticity and survival of Staphylococcus aureus in challenging environments. Although many S. aureus plasmids have been described, still few studies portray the plasmid content of a given S. aureus population. The aim of this work was to characterize the plasmids carried by a collection of 53 S. aureus isolates collected in a large hospital in Lisbon, Portugal, and investigate their role in conferring resistance to several antimicrobial agents. Plasmids were present in 44 out of the 53 isolates and were grouped into eleven AccI restriction profiles...
June 16, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Andreia N Horácio, Catarina Silva-Costa, Jorge Diamantino-Miranda, Joana P Lopes, Mario Ramirez, José Melo-Cristino
Among the 1660 isolates recovered from invasive pneumococcal disease (IPD) in adults (> = 18 yrs) in 2008-2011, a random sample of ≥50% of each serotype (n = 871) was chosen for MLST analysis and evaluation for the presence and type of pilus islands (PIs). The genetic diversity was high with 206 different sequence types (STs) detected, but it varied significantly between serotypes. The different STs represented 80 clonal complexes (CCs) according to goeBURST with the six more frequent accounting for more than half (50...
2016: PloS One
Giovanna Morello, Roberta Imperatore, Letizia Palomba, Carmine Finelli, Giuseppe Labruna, Fabrizio Pasanisi, Lucia Sacchetti, Lorena Buono, Fabiana Piscitelli, Pierangelo Orlando, Vincenzo Di Marzo, Luigia Cristino
In the hypothalamic arcuate nucleus (ARC), proopiomelanocortin (POMC) neurons and the POMC-derived peptide α-melanocyte-stimulating hormone (α-MSH) promote satiety. POMC neurons receive orexin-A (OX-A)-expressing inputs and express both OX-A receptor type 1 (OX-1R) and cannabinoid receptor type 1 (CB1R) on the plasma membrane. OX-A is crucial for the control of wakefulness and energy homeostasis and promotes, in OX-1R-expressing cells, the biosynthesis of the endogenous counterpart of marijuana's psychotropic and appetite-inducing component Δ(9)-tetrahydrocannabinol, i...
April 26, 2016: Proceedings of the National Academy of Sciences of the United States of America
Thamara Beline, Isabella da Silva Vieira Marques, Adaias O Matos, Erika S Ogawa, Antônio P Ricomini-Filho, Elidiane C Rangel, Nilson Cristino da Cruz, Cortino Sukotjo, Mathew T Mathew, Richard Landers, Rafael L X Consani, Marcelo Ferraz Mesquita, Valentim Adelino Ricardo Barão
In this study, the authors tested the hypotheses that plasma electrolytic oxidation (PEO) and glow-discharge plasma (GDP) would improve the electrochemical, physical, chemical, and mechanical properties of commercially pure titanium (cpTi), and that blood protein adsorption on plasma-treated surfaces would increase. Machined and sandblasted surfaces were used as controls. Standard electrochemical tests were conducted in artificial saliva (pHs of 3.0, 6.5, and 9.0) and simulated body fluid. Surfaces were characterized by scanning electron microscopy, energy-dispersive spectroscopy, x-ray photoelectron spectroscopy, atomic force microscopy, x-ray diffraction, profilometry, Vickers microhardness, and surface energy...
March 2016: Biointerphases
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