Laura Botelho Merij, Luana Rocha da Silva, Lohanna Palhinha, Milena Tavares Gomes, Paula Ribeiro Braga Dib, Remy Martins-Gonçalves, Kemily Toledo-Quiroga, Marcus Antônio Raposo-Nunes, Fernanda Brandi Andrade, Sharon de Toledo Martins, Ana Lúcia Rosa Nascimento, Vinicius Novaes Rocha, Lysangela Ronalte Alves, Patrícia T Bozza, Monique Ramos de Oliveira Trugilho, Eugenio D Hottz
BACKGROUND: Blood plasma is the main source of extracellular vesicles (EV) in clinical studies aiming the identification of biomarkers and the investigation of pathophysiological processes, especially regarding EV roles in inflammation and thrombosis. However, EV isolation from plasma has faced the fundamental issue of lipoprotein contamination, representing an important bias since lipoproteins are highly abundant and also modulate cell signaling, metabolism and thromboinflammation. OBJECTIVE: Here we aimed to isolate plasma EVs after depleting lipoproteins, improving sample purity and EV thromboinflammatory analysis...
January 24, 2024: Journal of Thrombosis and Haemostasis: JTH