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claudin 5

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https://www.readbyqxmd.com/read/28445614/two-common-human-cldn5-alleles-encode-different-open-reading-frames-but-produce-one-protein-isoform
#1
Ronald M Cornely, Barbara Schlingmann, Whitney S Shepherd, Joshua D Chandler, David C Neujahr, Michael Koval
Claudins provide tight junction barrier selectivity. The human CLDN5 gene contains a high-frequency single-nucleotide polymorphism (rs885985), where the G allele codes for glutamine (Q) and the A allele codes for an amber stop codon. Thus, these different CLDN5 alleles define nested open reading frames (ORFs) encoding claudin-5 proteins that are 303 or 218 amino acids in length. Interestingly, human claudin-16 and claudin-23 also have long ORFs. The long form of claudin-5 contrasts with the majority of claudin-5 proteins in the National Center for Biotechnology Information protein database, which are less than 220 amino acids in length...
April 26, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28431985/quercetin-interferes-with-the-fluid-volume-and-receptivity-development-of-the-uterus-in-rats-during-the-peri-implantation-period
#2
Huma Shahzad, Nelli Giribabu, Kamarulzaman Karim, Normadiah Kassim, Sekaran Muniandy, Kilari Eswar Kumar, Naguib Salleh
HYPOTHESIS: Quercetin could induce changes to the fluid volume and receptivity development of the uterus during peri-implantation period. METHODS: Female rats were treated with quercetin (10, 25 and 50mg/kg/day) subcutaneously beginning from day-1 pregnancy. Uterus was harvested at day-4 (following three days quercetin treatment) for morphological, ultra-structural, protein and mRNA expressional changes and plasma sex-steroid levels analyses. In another cohort of rats, implantation rate was determined at day-6 (following five days quercetin treatment)...
April 18, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28421347/tu-100-exerts-a-protective-effect-against-bacterial-translocation-by-maintaining-the-tight-junction
#3
Chie Takasu, Wubetu Gizachew Yismaw, Nobuhiro Kurita, Kozo Yoshikawa, Hideya Kashihara, Toru Kono, Mitsuo Shimada
PURPOSE: We previously reported that TU-100 suppresses irinotecan hydrochloride (CPT-11)-induced inflammatory cytokines and apoptosis. However, the mechanism underlying this effect has not been fully elucidated. The aim of this study was to further clarify the mechanism of CPT-11-induced bacterial translocation (BT) and the effect of TU-100 on BT. METHODS: Cell cytotoxicity was assessed in vitro by a WST-8 assay. For the in vivo experiments, rats were randomly divided into 3 groups: the control group, the CPT-11 group (250 mg/kg i...
April 18, 2017: Surgery Today
https://www.readbyqxmd.com/read/28415153/polar-and-charged-extracellular-residues-conserved-among-barrier-forming-claudins-contribute-to-tight-junction-strand-formation
#4
Anna Piontek, Jan Rossa, Jonas Protze, Hartwig Wolburg, Caroline Hempel, Dorothee Günzel, Gerd Krause, Jörg Piontek
Claudins (Cldn) form the backbone of tight junction (TJ) strands and thereby regulate paracellular permeability for solutes and water. Polymeric strands are formed by homo- and heterophilic cis- and trans-interactions between claudin protomers. Crystal structures of some claudins have been resolved; however, the mechanism by which claudins assemble into TJ strands remains unclear. To elucidate strand architecture, TJ-like strands were reconstituted in HEK293 cells by claudin transfection. Determinants of prototypic, classic barrier-forming claudins (Cldn1, -3, and -5) involved in strand formation were analyzed by mutagenesis...
April 17, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28407791/accelerated-differentiation-of-human-induced-pluripotent-stem-cells-to-blood-brain-barrier-endothelial-cells
#5
Emma K Hollmann, Amanda K Bailey, Archit V Potharazu, M Diana Neely, Aaron B Bowman, Ethan S Lippmann
BACKGROUND: Due to their ability to limitlessly proliferate and specialize into almost any cell type, human induced pluripotent stem cells (iPSCs) offer an unprecedented opportunity to generate human brain microvascular endothelial cells (BMECs), which compose the blood-brain barrier (BBB), for research purposes. Unfortunately, the time, expense, and expertise required to differentiate iPSCs to purified BMECs precludes their widespread use. Here, we report the use of a defined medium that accelerates the differentiation of iPSCs to BMECs while achieving comparable performance to BMECs produced by established methods...
April 13, 2017: Fluids and Barriers of the CNS
https://www.readbyqxmd.com/read/28405313/artificial-rearing-influences-the-morphology-permeability-and-redox-state-of-the-gastrointestinal-tract-of-low-and-normal-birth-weight-piglets
#6
Hans Vergauwen, Jeroen Degroote, Sara Prims, Wei Wang, Erik Fransen, Stefaan De Smet, Christophe Casteleyn, Steven Van Cruchten, Joris Michiels, Chris Van Ginneken
BACKGROUND: In this study the physiological implications of artificial rearing were investigated. Low (LBW) and normal birth weight (NBW) piglets were compared as they might react differently to stressors caused by artificial rearing. In total, 42 pairs of LBW and NBW piglets from 16 litters suckled the sow until d19 of age or were artificially reared starting at d3 until d19 of age. Blood and tissue samples that were collected after euthanasia at 0, 3, 5, 8 and 19 d of age. Histology, ELISA, and Ussing chamber analysis were used to study proximal and distal small intestine histo-morphology, proliferation, apoptosis, tight junction protein expression, and permeability...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28396190/protective-effect-of-4-methoxy-benzyl-alcohol-on-the-blood-brain-barrier-after-cerebral-ischemia-reperfusion-injury
#7
Fangyan He, Xiaohua Duan, Rong Dai, Yan Li, Qing Lin
Damage of the blood-brain barrier (BBB) during the process of cerebral ischemic injury is a key factor that influences the therapeutic efficacy to the cerebral ischemic injury. This work was designed to investigate the mechanisms underlying the protective effects of 4-methoxy benzyl alcohol (4-MA) on the BBB by developing a cerebral ischemia/reperfusion model of rats (MCAO/R). The MCAO/R was developed through a thread embolism method. The neurologic scales, the brain infarct rate, and the Evans blue (EB) contents of the brains were detected...
April 7, 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28391269/rebeccamycin-attenuates-tnf-%C3%AE-induced-intestinal-epithelial-barrier-dysfunction-by-inhibiting-myosin-light-chain-kinase-production
#8
Akihiro Watari, Yuta Sakamoto, Kota Hisaie, Kazuki Iwamoto, Miho Fueta, Kiyohito Yagi, Masuo Kondoh
BACKGROUND/AIMS: Although proinflammatory cytokine-induced disruption of intestinal epithelial barrier integrity is associated with intestinal inflammatory disease, effective treatment for barrier dysfunction is lacking. Previously, we demonstrated that rebeccamycin alleviates epithelial barrier dysfunction induced by inflammatory cytokines in Caco-2 cell monolayers; however, the underlying mechanism remained unclear. Here, we investigated the mechanism by which rebeccamycin protects the epithelial barrier function of Caco-2 cells exposed to TNF-α...
April 7, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28388666/picroside-ii-protects-the-blood-brain-barrier-by-inhibiting-the-oxidative-signaling-pathway-in-cerebral-ischemia-reperfusion-injury
#9
Li Zhai, Min Liu, Tingting Wang, Hongyan Zhang, Shan Li, Yunliang Guo
BACKGROUND AND PURPOSE: Thrombolysis is used to improve cerebral circulation; at the same time, neuroprotective drugs such as antioxidants should also be used. The aim of these experiments was to explore the protective mechanism of an antioxidant, picroside II, on the blood-brain barrier (BBB) after cerebral ischemia-reperfusion (CI/R) injury. METHODS: To observe the antagonistic effect of picroside II on CI/R damage, the neurological deficit score and the infarct volume were measured...
2017: PloS One
https://www.readbyqxmd.com/read/28386329/inhibiting-endoplasmic-reticulum-stress-by-lithium-chloride-contributes-to-the-integrity-of-blood-spinal-cord-barrier-and-functional-recovery-after-spinal-cord-injury
#10
Zili He, Yulong Zhou, Qingqing Wang, Jiawei Li, Zengming Zheng, Jian Chen, Hongyu Zhang, Zhouguang Wang, Huazi Xu, Jian Xiao
Endoplasmic reticulum (ER) stress play important roles in the spinal cord injury (SCI), which including blood-spinal cord barrier (BSCB) disruption. Lithium chloride (LiCl) is a clinical drug for bipolar mood disorders and contributes to neuroprotection. This study aims to investigate the effects of LiCl on BSCB disruption and the ER stress pathway induced by spinal cord injury. We examined the integrity of the BSCB with Evans Blue dye and macrophages extravasation, measured the microvessels loss, the junction proteins degeneration, the activation ER stress, and the locomotor function recovery...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28379489/new-thrombolytic-strategy-providing-neuroprotection-in-experimental-ischemic-stroke-mmp10-alone-or-in-combination-with-tpa
#11
C Roncal, S Martinez de Lizarrondo, A Salicio, A Chevilley, J A Rodriguez, A Rosell, P O Couraud, B Weksler, J Montaner, D Vivien, J A Páramo, J Orbe
Aim: Considering that MMP-10 improves stroke injury, we have examined the therapeutic and protective effect of MMP10 and tPA/MMP10 as clot-dissolving and neuroprotective agent in an experimental model of ischemic stroke and studied in vitro the molecular pathways involved in MMP10-mediated effects. Methods and Results: Cerebral ischemia was induced by the local injection of thrombin into the middle cerebral artery followed by reperfusion with MMP10 (6.5µg/Kg) and tPA (10mg/Kg) alone or in combination with MMP10...
April 3, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28379469/in-vivo-intrabursal-administration-of-bioactive-lipid-sphingosine-1-phosphate-enhances-vascular-integrity-in-a-rat-model-of-ovarian-hyperstimulation-syndrome-ohss
#12
Mariana Di Pietro, Natalia Pascuali, Leopoldina Scotti, Griselda Irusta, Diana Bas, María May, Marta Tesone, Dalhia Abramovich, Fernanda Parborell
STUDY QUESTION: Can the bioactive lipid sphingosine-1 phosphate (S1P) act as an endothelial barrier-enhancing molecule and, in turn, restore the vascular integrity and homeostasis in a rat model of ovarian hyperstimulation syndrome (OHSS). STUDY ANSWER: In vivo administration of S1P may prevent the early onset of OHSS and decrease its severity. WHAT IS KNOWN ALREADY: Although advances in the prediction and treatment of OHSS have been made, complete prevention has not been possible yet...
April 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28377727/transcriptomic-analysis-of-the-claudin-interactome-in-malignant-pleural-mesothelioma-evaluation-of-the-effect-of-disease-phenotype-asbestos-exposure-and-cdkn2a-deletion-status
#13
Erasmia Rouka, Georgios D Vavougios, Evgeniy I Solenov, Konstantinos I Gourgoulianis, Chrissi Hatzoglou, Sotirios G Zarogiannis
Malignant pleural mesothelioma (MPM) is a highly aggressive tumor primarily associated with asbestos exposure. Early detection of MPM is restricted by the long latency period until clinical presentation, the ineffectiveness of imaging techniques in early stage detection and the lack of non-invasive biomarkers with high sensitivity and specificity. In this study we used transcriptome data mining in order to determine which CLAUDIN (CLDN) genes are differentially expressed in MPM as compared to controls. Using the same approach we identified the interactome of the differentially expressed CLDN genes and assessed their expression profile...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28373092/cyclosporine-a-alleviated-matrix-metalloproteinase-9-associated-blood-brain-barrier-disruption-after-subarachnoid-hemorrhage-in-mice
#14
Pengyu Pan, Xuan Zhang, Qiang Li, Hengli Zhao, Jie Qu, John H Zhang, Xin Liu, Hua Feng, Yujie Chen
The aim of this study was to investigate whether Cyclosporine A (CsA) attenuates early brain injury by alleviating matrix metalloproteinase 9 (MMP-9) associated blood-brain barrier (BBB) disruption after subarachnoid hemorrhage (SAH). A standard intravascular perforation model was used to produce the experimental SAH in C57B6J mice. Dosages of 5mg/kg, 10mg/kg and 15mg/kg CsA were evaluated for effects on neurological score, brain water content, Evans blue extravasation and fluorescence, P-p65, MMP-9 and BBB components' alterations after SAH...
March 31, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28367588/effect-of-long-term-in-vitro-lithium-exposure-on-mrna-levels-of-claudin-3-cyp1a1-abcg2-and-gstm3-genes-in-the-hcmec-d3-human-brain-endothelial-cell-line
#15
Ramzi Shawahna, Kayathiri Ganeshamoorthy, Luo Huilong, Jean-Michel Scherrmann, Pierre-Olivier Couraud, Xavier Declèves
BACKGROUND AND OBJECTIVES: Lithium chloride (LiCl) has been shown to improve the tightness of brain endothelial cell monolayers in vitro by inhibition of the GSK-3β enzyme, activation of the Wnt/beta-catenin pathway and regulation of tight junction (TJ) protein expression. However, the effect of LiCl on the drug transporters and drug-metabolizing enzymes has not been addressed so far. The hCMEC/D3 cell line is a validated in vitro BBB model expressing transporters and drug-metabolizing enzymes (phase 1 and 2)...
April 3, 2017: European Journal of Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/28367226/protective-effect-of-tang-wang-one-decoction-on-the-retinal-vessels-of-diabetic-rats
#16
Xinyun Kou, Shufei Yang, Yali Qin, Chao Yang, Tingting Deng, Dan Luo, Ming Jin
Objective. This study aimed to determine the influence of Tang Wang One Decoction (TWOD) on the retinal vessels of diabetic rats. Methods. The hemorheology of diabetic rats was observed. Morphological studies of retinal vessels were conducted using optical microscopy and electron microscopy. Immunological histochemistry assay was used to measure the expression levels of MMP-9, occludin, and claudin-5. Results. Obvious pathological damage was observed in the retinal vessels of diabetic rats. TWOD positively affected the hemorheology and morphology of retinal vessels...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28366783/intestinal-immune-responses-of-jian-carp-against-aeromonas-hydrophila-depressed-by-choline-deficiency-varied-change-patterns-of-mrna-levels-of-cytokines-tight-junction-proteins-and-related-signaling-molecules-among-three-intestinal-segments
#17
Pei Wu, Yang Liu, Wei-Dan Jiang, Jun Jiang, Yong-An Zhang, Xiao-Qiu Zhou, Lin Feng
This study aimed to investigate the effects of choline deficiency on intestinal inflammation of fish after Aeromonas hydrophila infection and the potential molecular mechanisms. Juvenile Jian carp (Cyprinus carpio var. Jian) were fed two diets containing choline at 165 (deficient group) and 607 mg/kg diet respectively for 65 days. Choline deficiency decreased intestinal lysozyme activity, C3 and IgM contents, increased acid phosphatase activity, downregulated mRNA levels of antimicrobial peptides [liver-expressed antimicrobial peptide (LEAP) 2A, LEAP-2B, hepcidin and defensin], cytokines [interleukin (IL) 6a, tumor necrosis factor α (TNF-α), interferon γ2b (IFN-γ2b), IL-6b and transforming growth factor β2 (TGF-β2) only in proximal intestine, IL-10 in mid and distal intestine], immune-related signaling molecules [Toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (MyD88), nuclear factor kappa B (NF-κB), inhibitor of NF-κB (IκB), Janus kinase 3 (JAK3), and signal transducers and activators of transcription 5 (STAT5)], tight junction proteins (claudin 3b, claudin 3c, claudin 11 and occludin), and mitogen-activated protein kinases p38 (p38(MAPK)) in proximal and distal intestine of juvenile Jian carp after A...
March 30, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28364214/frailty-and-long-term-mortality-of-older-breast-cancer-patients-calgb-369901-alliance
#18
Jeanne S Mandelblatt, Ling Cai, George Luta, Gretchen Kimmick, Jonathan Clapp, Claudine Isaacs, Brandeyln Pitcher, William Barry, Eric Winer, Stephen Sugarman, Clifford Hudis, Hyman Muss, Harvey J Cohen, Arti Hurria
PURPOSE: Breast cancer patients aged 65+ ("older") vary in frailty status. We tested whether a deficits accumulation frailty index predicted long-term mortality. METHODS: Older patients (n = 1280) with non-metastatic, invasive breast cancer were recruited from 78 Alliance sites from 2004 to 2011, with follow-up to 2015. Frailty categories (robust, pre-frail, and frail) were based on 35 baseline illness and function items. Cox proportional hazards and competing risk models were used to calculate all-cause and breast cancer-specific mortality for up to 7 years, respectively...
March 31, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28355299/mild-hypothermia-alleviates-brain-oedema-and-blood-brain-barrier-disruption-by-attenuating-tight-junction-and-adherens-junction-breakdown-in-a-swine-model-of-cardiopulmonary-resuscitation
#19
Jiebin Li, Chunsheng Li, Wei Yuan, Junyuan Wu, Jie Li, Zhenhua Li, Yongzhen Zhao
Mild hypothermia improves survival and neurological recovery after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR). However, the mechanism underlying this phenomenon is not fully elucidated. The aim of this study was to determine whether mild hypothermia alleviates early blood-brain barrier (BBB) disruption. We investigated the effects of mild hypothermia on neurologic outcome, survival rate, brain water content, BBB permeability and changes in tight junctions (TJs) and adherens junctions (AJs) after CA and CPR...
2017: PloS One
https://www.readbyqxmd.com/read/28338398/dexamethasone-suppresses-jmjd3-gene-activation-via-a-putative-negative-glucocorticoid-response-element-and-maintains-integrity-of-tight-junctions-in-brain-microvascular-endothelial-cells
#20
Wonho Na, Jee Y Shin, Jee Y Lee, Sangyun Jeong, Won-Sun Kim, Tae Y Yune, Bong-Gun Ju
The blood-brain barrier (BBB) exhibits a highly selective permeability to support the homeostasis of the central nervous system (CNS). The tight junctions in the BBB microvascular endothelial cells seal the paracellular space to prevent diffusion. Thus, disruption of tight junctions results in harmful effects in CNS diseases and injuries. It has recently been demonstrated that glucocorticoids have beneficial effects on maintaining tight junctions in both in vitro cell and in vivo animal models. In the present study, we found that dexamethasone suppresses the expression of JMJD3, a histone H3K27 demethylase, via the recruitment of glucocorticoid receptor α (GRα) and nuclear receptor co-repressor (N-CoR) to the negative glucocorticoid response element (nGRE) in the upstream region of JMJD3 gene in brain microvascular endothelial cells subjected to TNFα treatment...
January 1, 2017: Journal of Cerebral Blood Flow and Metabolism
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