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https://www.readbyqxmd.com/read/29773101/-microvesicles-derived-from-lps-induced-microglia-aggravate-the-injury-of-tight-junction-in-rat-brain-microvascular-endothelial-cells-under-oxygen-glucose-deprivation
#1
Yanni Lyu, Longsheng Fu, Yanming Li, Jinhua Wen, Xiaohua Wei, Yisong Qian
Objective To study the effect of the administration of the circulating microvesicles (MV) obtained from lipopolysaccharide (LPS)-stimulated microglia supernatant into rat brain endothelial cells (RBECs) on the injury of tight junction in RBECs under the condition of oxygen-glucose deprivation (OGD) as well as the underlying mechanism. Methods The circulating MV was isolated from the supernatant of microglia stimulated with LPS 1 mg/L for 24 hours and subjected to morphological identification. The expression of miR-27a in MV was detected by real-time PCR...
March 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29770867/dietary-supplementation-with-an-amino-acid-blend-enhances-intestinal-function-in-piglets
#2
Dan Yi, Baocheng Li, Yongqing Hou, Lei Wang, Di Zhao, Hongbo Chen, Tao Wu, Ying Zhou, Binying Ding, Guoyao Wu
The traditionally classified nutritionally non-essential amino acids are now known to be insufficiently synthesized for maximal growth and optimal health in piglets. This study determined the effects of dietary supplementation with an amino acid blend (AAB; glutamate:glutamine:glycine:arginine:N-acetylcysteine = 5:2:2:1:0.5) on piglet growth performance and intestinal functions. Sixteen piglets (24-day-old) were randomly assigned to a corn and soybean meal-based diet supplemented with 0.99% alanine (isonitrogenous control) or 1% AAB...
May 16, 2018: Amino Acids
https://www.readbyqxmd.com/read/29764545/-effects-of-n-butylphthalide-on-the-expressions-of-zo-1-and-claudin-5-in-blood-brain-barrier-of-rats-with-acute-carbon-monoxide-poisoning
#3
Li Wang, Xiaoyu Ding, Mingjun Bi, Jinglin Wang, Yong Zou, Jiyou Tang, Qin Li
OBJECTIVE: To explore the effects of N-butylphthalide on the expressions of ZO-1 and claudin-5 in blood-brain barrier (BBB) in rats with acute carbon monoxide (CO) poisoning. METHODS: A total of 144 adult healthy male Sprague-Dawley (SD) rats were randomly divided into normal control group, CO poisoning group, and NBP treatment group, with 48 rats in each group. The acute CO poisoning model was reproduced in hyperbaric oxygen chamber, and all model rats were given hyperbaric oxygen therapy once daily...
May 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29755115/endothelial-akt1-loss-promotes-prostate-cancer-metastasis-via-%C3%AE-catenin-regulated-tight-junction-protein-turnover
#4
Fei Gao, Abdulrahman Alwhaibi, Sandeep Artham, Arti Verma, Payaningal R Somanath
BACKGROUND: Cancer research, in general, is focused on targeting tumour cells to limit tumour growth. These studies, however, do not account for the specific effects of chemotherapy on tumour endothelium, in turn, affecting metastasis. METHODS: We determined how endothelial deletion of Akt1 promotes prostate cancer cell invasion in vitro and metastasis to the lungs in vivo in endothelial-specific Akt1 knockdown mice. RESULTS: Here we show that metastatic human PC3 and DU145 prostate cancer cells invade through Akt1-deficient human lung endothelial cell (HLEC) monolayer with higher efficiency compared to control HLEC...
May 14, 2018: British Journal of Cancer
https://www.readbyqxmd.com/read/29754847/feasibility-of-classification-of-triple-negative-breast-cancer-by-immunohistochemical-surrogate-markers
#5
Sewha Kim, Byung-In Moon, Woosung Lim, Sanghui Park, Min Sun Cho, Sun Hee Sung
INTRODUCTION: Recently, Burstein et al identified 4 stable molecular subtypes of triple negative breast cancer (TNBC) by mRNA profiling: luminal androgen receptor (LAR), mesenchymal (MES), basal-like immune-activated (BLIA), and basal-like immune-suppressive (BLIS) types. The purpose of this study was to assess the feasibility of immunohistochemistry (IHC) surrogate panel in classifying the TNBC molecular subtypes using a large cohort of TNBC retrieved from a single institution. MATERIALS AND METHODS: IHC for androgen receptor [AR], claudin-3, E-cadherin, cytokeratin 5/6 [CK5/6], epidermal growth factor receptor [EGFR], indoleamine 2,3-dioxygenase 1 [IDO1], and Forkhead box C1 [FOXC1] were performed using the tissue microarray constructed from 200 TNBC samples...
March 23, 2018: Clinical Breast Cancer
https://www.readbyqxmd.com/read/29749584/droplet-digital-pcr-detects-high-rate-of-tp53-r249s-mutants-in-cell-free-dna-of-middle-african-patients-with-hepatocellular-carcinoma
#6
Agnès Marchio, Marie Amougou Atsama, Aubin Béré, Narcisse-Patrice Komas, Dominique Noah Noah, Paul Jean Adrien Atangana, Serge-Magloire Camengo-Police, Richard Njouom, Claudine Bekondi, Pascal Pineau
Hepatocellular carcinoma (HCC) is still a major killing malignancy in sub-Saharan Africa. Lifelong intoxication with aflatoxin B1 is considered as one of the primary causes of this situation. The role of aflatoxin in HCC from a given population is commonly estimated through the prevalence of R249S mutation of TP53, a hallmark for previous exposure to the mycotoxin. However, the role of AFB1 is barely known in large part of Africa. We conducted a survey on circulating cell-free DNA from 149 patients with HCC and 213 control subjects with and without liver diseases from Cameroon and Central African Republic using droplet digital PCR technique...
May 10, 2018: Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/29749528/methylation-of-the-claudin%C3%A2-3-promoter-predicts-the-prognosis-of-advanced-gastric-adenocarcinoma
#7
Zhenzhen Zhang, Weixing Yu, Shuqin Chen, Yupeng Chen, Linying Chen, Sheng Zhang
Claudin‑3 expression is associated with gastric cancer progression, but the role of epigenetic modifications remains unclear. We investigated methylation of the claudin‑3 promoter and expression profiles in gastric adenocarcinoma and their associations with clinicopathological characteristics and prognosis of the patients. A total of 122 patients with advanced gastric cancer [stage IIB‑IV, with lymph node (LN) metastasis] were enrolled. Each patient provided 4 tissue samples: normal gastric epithelium, intestinal metaplasia, primary tumor and metastatic LN...
May 2, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29747348/-cerebral-cavernous-malformation-3-gene-deficiency-promotes-early-changes-in-alzheimer-disease-like-lesions-induced-by-low-lead-exposure
#8
S Wu, D Xia, H X Liu, H P Zhao, X H Wang, Y F Gao, Z Q Zhao, C Xiao, X M Xing, Y He
Objective: To investigate the effects of cerebral cavernous malformation 3 (CCM3) gene knockout on the lead exposure-induced blood-brain barrier malfunction in mice brain, and the relationship between CCM3 knockout and the Alzheimer's disease (AD). Methods: Wide type (WT) mice and CCM3(+)/- mice were divided into 4 groups, control group and lead exposed group in WT as well as CCM3(+/-) mice. Lead exposed groups were treated with 0.05% lead acetate in drinking water for 12 weeks, while control group drink deionized water freely...
May 6, 2018: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29745557/-effects-of-silencing-snail1-gene-on-the-expression-of-tight-junction-proteins-and-the-migration-ability-of-hep-2-cells
#9
Shuangfeng Liu, Yang Shen, Tang Feng, Xiaoheng Liu
To investigate the effects of Snail1 gene silence on the expression of tight junction proteins and the migration ability of Hep-2 cells, Hep-2 cells were transfected with plasmids which is containing the shRNA of Snail1 gene, and cultured till the cells could be passaged stably (named Sh-snail1 cells). The expression of tight junction proteins (ZO-1, Occludin, Claudin-5) were detected by Western blot. The migration ability of Sh-snail1 cells was investigated by wound healing assay, and the protein expression of members of RhoGTPase family (RhoA, Cdc42) was detected by Western blot, which is closely related to the migration ability...
August 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29731129/early-onset-pediatric-atopic-dermatitis-is-characterized-by-t-h-2-t-h-17-t-h-22-centered-inflammation-and-lipid-alterations
#10
Patrick M Brunner, Ariel Israel, Ning Zhang, Alexandra Leonard, Huei-Chi Wen, Thy Huynh, Gary Tran, Sarah Lyon, Giselle Rodriguez, Supriya Immaneni, Annette Wagner, Xiuzhong Zheng, Yeriel D Estrada, Hui Xu, James G Krueger, Amy S Paller, Emma Guttman-Yassky
BACKGROUND: Although atopic dermatitis (AD) often starts in early childhood, detailed tissue profiling of early-onset AD in children is lacking, hindering therapeutic development for this patient population with a particularly high unmet need for better treatments. OBJECTIVE: We sought to globally profile the skin of infants with AD compared with that of adults with AD and healthy control subjects. METHODS: We performed microarray, RT-PCR, and fluorescence microscopy studies in infants and young children (<5 years old) with early-onset AD (<6 months disease duration) compared with age-matched control subjects and adults with longstanding AD...
April 19, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29728011/the-protective-roles-of-l-borneolum-d-borneolum-and-synthetic-borneol-in-cerebral-ischaemia-via-modulation-of-the-neurovascular-unit
#11
Taiwei Dong, Nian Chen, Xiao Ma, Jian Wang, Jing Wen, Qian Xie, Rong Ma
OBJECTIVE: Borneol has been used to treat stroke in China since ancient times. In our previous research, we demonstrated the effect of borneol on cerebral ischaemia injury via meta-analysis. The neurovascular unit (NVU) is the structural basis of the preservation of the brain microenvironment and is believed to be a promising target in treating stroke. In this research, we explored the roles of three kinds of borneol, namely, L-borneolum (B1), D-borneolum (B2) and synthetic borneol (B3), in the NVU with permanent middle cerebral artery occluded (pMCAO) rats...
June 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29726306/secreted-giardia-intestinalis-cysteine-proteases-disrupt-intestinal-epithelial-cell-junctional-complexes-and-degrade-chemokines
#12
Jingyi Liu, Showgy Ma'ayeh, Dimitra Peirasmaki, Britta Lundström-Stadelmann, Lars Hellman, Staffan G Svärd
Giardiasis is a common diarrheal disease caused by the protozoan parasite Giardia intestinalis. Cysteine proteases (CPs) are acknowledged as virulence factors in Giardia but their specific role in the molecular pathogenesis of disease is not known. Herein, we aimed to characterize the three main secreted CPs (CP14019, CP16160 and CP16779), which were identified by mass spectrometry in the medium during interaction with intestinal epithelial cells (IECs) in vitro. First, the CPs were epitope-tagged and localized to the endoplasmic reticulum and cytoplasmic vesicle-like structures...
December 31, 2018: Virulence
https://www.readbyqxmd.com/read/29723978/ergot-alkaloids-at-doses-close-to-eu-regulatory-limits-induce-alterations-of-the-liver-and-intestine
#13
Viviane Mayumi Maruo, Ana Paula Bracarense, Jean-Paul Metayer, Maria Vilarino, Isabelle P Oswald, Philippe Pinton
An increase in the occurrence of ergot alkaloids (EAs) contamination has been observed in North America and Europe in recent years. These toxins are well known for their effects on the circulatory and nervous systems. The aim of this study was to investigate the effect of EAs on the liver and on the intestine using the pig both as a target species and as a non-rodent model for human. Three groups of 24 weaned piglets were exposed for 28 days to control feed or feed contaminated with 1.2 or 2.5 g of sclerotia/kg, i...
May 1, 2018: Toxins
https://www.readbyqxmd.com/read/29722942/-role-of-tgf-%C3%AE-1-in-sertoli-cells-and-tight-junction
#14
Jiang-Tao Lou, Ren-Xiong Wei, Lang-Lang Yu, Jian-Wei Chen, Yun Cui
Objective: To explore the role of TGF-β1 in the proliferation and apoptosis of Sertoli cells and its effect on the expressions of tight junction-related proteins and genes in rats. METHODS: Rat Sertoli cells were isolated in vitro, primarily cultured, and divided into groups A (blank control), B (TGF-β1 receptor blocker), C (TGF-β1), and D (TGF-β1 + receptor blocker). The proliferation and apoptosis of the cells were detected by CCK-8 and flow cytometry, respectively...
June 2017: Zhonghua Nan Ke Xue, National Journal of Andrology
https://www.readbyqxmd.com/read/29719498/anxiety-and-10-year-risk-of-incident-dementia-an-association-shaped-by-depressive-symptoms-results-of-the-prospective-three-city-study
#15
Marion Mortamais, Meriem Abdennour, Valérie Bergua, Christophe Tzourio, Claudine Berr, Audrey Gabelle, Tasnime N Akbaraly
Background: Anxiety is common in patients with cognitive impairment and dementia. However, whether anxiety is a risk factor for dementia is still not known. We aimed to examine the association between trait anxiety at baseline and the 10-year risk of incident dementia to determine to which extent depressive symptoms influence this relationship in the general population. Methods: Data came from 5,234 community-dwelling participants from the Three-City prospective cohort study, aged 65 years at baseline and followed over 10 years...
2018: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29713894/fluoxetine-is-neuroprotective-in-early-brain-injury-via-its-anti-inflammatory-and-anti-apoptotic-effects-in-a-rat-experimental-subarachnoid-hemorrhage-model
#16
Hui-Min Hu, Bin Li, Xiao-Dong Wang, Yun-Shan Guo, Hua Hui, Hai-Ping Zhang, Biao Wang, Da-Geng Huang, Ding-Jun Hao
Fluoxetine, an anti-depressant drug, has recently been shown to provide neuroprotection in central nervous system injury, but its roles in subarachnoid hemorrhage (SAH) remain unclear. In this study, we aimed to evaluate whether fluoxetine attenuates early brain injury (EBI) after SAH. We demonstrated that intraperitoneal injection of fluoxetine (10 mg/kg per day) significantly attenuated brain edema and blood-brain barrier (BBB) disruption, microglial activation, and neuronal apoptosis in EBI after experimental SAH, as evidenced by the reduction of brain water content and Evans blue dye extravasation, prevention of disruption of the tight junction proteins zonula occludens-1, claudin-5, and occludin, a decrease of cells staining positive for Iba-1, ED-1, and TUNEL and a decline in IL-1β, IL-6, TNF-α, MDA, 3-nitrotyrosine, and 8-OHDG levels...
April 30, 2018: Neuroscience Bulletin
https://www.readbyqxmd.com/read/29708077/emerging-drugs-for-the-treatment-of-breast-cancer-brain-metastasis-a-review-of-patent-literature
#17
Maricruz Anaya-Ruiz, Cindy Bandala, Patricia Martinez-Morales, Gerardo Landeta, Rebeca D Martinez-Contreras, Nancy Martinez-Montiel, Martin Perez-Santos
BACKGROUND: Despite dramatic advances in cancer treatment that lead to long-term survival, there is an increasing number of patients presenting with clinical manifestations of cerebral metastasis in breast cancer, for whom only palliative treatment options exist. OBJECTIVE: The present review aims to provide to identify recent patens of breast cancer brain metastasis that may have application in improving cancer treatment. METHOD: Recent patents regarding the breast cancer brain metastasis were obtained from USPTO patent databases, Esp@cenet, Patentscope and Patent Inspiration®...
April 29, 2018: Recent Patents on Anti-cancer Drug Discovery
https://www.readbyqxmd.com/read/29700607/cerebrovascular-inflammation-is-associated-with-tau-pathology-in-guam-parkinsonism-dementia
#18
Petra Majerova, Ralph M Garruto, Andrej Kovac
Parkinsonism-dementia complex of Guam (Guam PDC) is a neurodegenerative disease with parkinsonism and early onset Alzheimer-like dementia. Guam PDC belongs to the family of neurodegenerative disorders, known as tauopathies, which are histopathologically characterized by abnormal deposition of microtubule-associated protein tau. While changes in the blood-brain barrier (BBB) in Alzheimer's disease are increasingly recognized, dysfunction of BBB in Guam PDC has not been extensively studied. In this study, we characterized cerebrovascular changes in the patients with Guam PDC...
April 26, 2018: Journal of Neural Transmission
https://www.readbyqxmd.com/read/29700418/smarca4-deficient-undifferentiated-uterine-sarcoma-malignant-rhabdoid-tumor-of-the-uterus-a-clinicopathologic-entity-distinct-from-undifferentiated-carcinoma
#19
David L Kolin, Fei Dong, Michele Baltay, Neal Lindeman, Laura MacConaill, Marisa R Nucci, Christopher P Crum, Brooke E Howitt
Small cell carcinoma of the ovary, hypercalcemic type is a rare, aggressive malignancy which usually occurs in young women and is characterized by mutations in SMARCA4, with few other alterations. We recently encountered uterine tumors with morphologic, immunohistochemical, and genetic similarities to small cell carcinoma of the ovary, hypercalcemic type. Herein we report the clinicopathologic and molecular features (using a targeted massively parallel sequencing [MPS] assay) of these tumors. The cases were diagnosed on cervical and endometrial biopsies (n = 2, 34, and 29 years) or hysterectomy and bilateral salpingo-oophorectomy (n = 3, 25, 33, and 58 years)...
April 26, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29700355/claudin-11-regulates-bone-homeostasis-via-bidirectional-ephb4-ephrinb2-signaling
#20
Jong Min Baek, Yoon-Hee Cheon, Sung Chul Kwak, Hong Young Jun, Kwon-Ha Yoon, Myeung Su Lee, Ju-Young Kim
Claudins (Cldns) are well-established components of tight junctions (TJs) that play a pivotal role in the modulation of paracellular permeability. Several studies have explored the physiologic aspects of Cldn family members in bone metabolism. However, the effect of Cldn11, a major component of central nervous system myelin, on bone homeostasis has not been reported. In this study, we demonstrate that Cldn11 is a potential target for bone disease therapeutics as a dual modulator of osteogenesis enhancement and osteoclastogenesis inhibition...
April 27, 2018: Experimental & Molecular Medicine
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