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claudin 5

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https://www.readbyqxmd.com/read/28528309/maintenance-of-claudin-3-expression-and-the-barrier-functions-of-intercellular-junctions-in-parotid-acinar-cells-via-the-inhibition-of-src-signaling
#1
Megumi Yokoyama, Takanori Narita, Hajime Sakurai, Osamu Katsumata-Kato, Hiroshi Sugiya, Junko Fujita-Yoshigaki
OBJECTIVES: Salivary acinar and duct cells show different expression patterns of claudins, which may reflect their different functions. To study the role of claudins in saliva secretion, we examined alterations in the expression patterns of cell adhesion molecules in parotid glands of γ-irradiated rats and analyzed the influence of those changes on intercellular barrier function using primary cultures of parotid acinar cells. DESIGN: Rats were γ-irradiated with doses of 5, 15 or 20Gy, and expression levels of cell adhesion molecules were examined by immunoblotting analysis...
May 15, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28523565/blood-brain-barrier-permeability-is-exacerbated-in-experimental-model-of-hepatic-encephalopathy-via-mmp-9-activation-and-downregulation-of-tight-junction-proteins
#2
Saurabh Dhanda, Rajat Sandhir
The present study was designed to investigate the mechanisms involved in blood-brain barrier (BBB) permeability in bile duct ligation (BDL) model of chronic hepatic encephalopathy (HE). Four weeks after BDL surgery, a significant increase was observed in serum bilirubin levels. Masson trichrome staining revealed severe hepatic fibrosis in the BDL rats. (99m)Tc-mebrofenin retention was increased in the liver of BDL rats suggesting impaired hepatobiliary transport. An increase in permeability to sodium fluorescein, Evans blue, and fluorescein isothiocyanate (FITC)-dextran along with increase in water and electrolyte content was observed in brain regions of BDL rats suggesting disrupted BBB...
May 18, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28512723/low-dose-dual-isotope-procedure-planed-for-myocardial-perfusion-czt-spect-and-assessed-through-a-head-to-head-comparison-with-a-conventional-single-isotope-protocol
#3
Laetitia Imbert, Véronique Roch, Charles Merlin, Wassila Djaballah, Florent Cachin, Mathieu Perrin, Marine Claudin, Antoine Verger, Henri Boutley, Gilles Karcher, Pierre-Yves Marie
PURPOSE OF THE REPORT: This study aimed at assessing an original low-dose dual-isotope procedure in which the abnormal stress Tc-99m Sestamibi SPECT is followed by rest Tl-201 SPECT, along with a head-to-head comparison with a single-isotope procedure. METHODS AND RESULTS: One hundred two patients, referred for a low-dose stress-SPECT with Sestamibi (123 ± 20 MBq) on a CZT camera and for whom a rest Sestamibi SPECT was warranted, had an additional Tl-201 rest-SPECT (52 ± 5 MBq) between stress and rest Sestamibi SPECT recordings...
May 16, 2017: Journal of Nuclear Cardiology: Official Publication of the American Society of Nuclear Cardiology
https://www.readbyqxmd.com/read/28512445/the-therapeutic-value-of-bone-marrow-derived-endothelial-progenitor-cell-transplantation-after-intracerebral-hemorrhage-in-rats
#4
Rui Zhang, Jing Yang, Jingjing Yuan, Bo Song, Yan Wang, Yuming Xu
AIMS: To study the effect of endothelial progenitor cell (EPC) treatment on intracerebral hemorrhage (ICH) in rats and elucidate possible mechanisms. METHODS: The rats were randomly divided into three groups: (1) EPC group: ICH + EPC, (2) phosphate-buffered saline group: ICH + PBS, and (3) sham group. EPCs were transplanted intravenously 6 h after ICH. Modified neurological severity score was used to evaluate neurological function. Blood-brain barrier (BBB) integrity was evaluated...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28505395/claudin-peptidomimetics-modulate-tissue-barriers-for-enhanced-drug-delivery
#5
Sophie Dithmer, Christian Staat, Carolin Müller, Min-Chi Ku, Andreas Pohlmann, Thoralf Niendorf, Nora Gehne, Petra Fallier-Becker, Ágnes Kittel, Fruzsina R Walter, Szilvia Veszelka, Maria A Deli, Rosel Blasig, Reiner F Haseloff, Ingolf E Blasig, Lars Winkler
The blood-brain barrier (BBB) formed by the microvascular endothelium limits cerebral drug delivery. The paraendothelial cleft is sealed by tight junctions (TJs) with a major contribution from claudin-5, which we selected as a target to modulate BBB permeability. For this purpose, drug-enhancer peptides were designed based on the first extracellular loop (ECL) of claudin-5 to allow transient BBB permeabilization. Peptidomimetics (C5C2 and derivatives, nanomolar affinity to claudin-5) size-selectively (≤40 kDa) and reversibly (12-48 h) increased the permeability of brain endothelial and claudin-5-transfected epithelial cell monolayers...
May 15, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28501330/virus-entry-and-replication-in-the-brain-precedes-blood-brain-barrier-disruption-during-intranasal-alphavirus-infection
#6
Matthew D Cain, Hamid Salimi, Yongfeng Gong, Lihua Yang, Samantha L Hamilton, James R Heffernan, Jianghui Hou, Mark J Miller, Robyn S Klein
Viral infections of the central nervous system (CNS) are often associated with blood-brain barrier (BBB) disruption, yet the impact of virus replication and immune cell recruitment on BBB integrity are incompletely understood. Using two-photon microscopy, we demonstrate that Venezuelan equine encephalitis virus (VEEV) strain TC83-GFP, a GFP expressing, attenuated strain with a G3A mutation within the 5' UTR that is associated with increased sensitivity to type I interferons (IFNs), does not directly impact BBB permeability...
May 1, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28498323/alkamides-from-anacyclus-pyrethrum-l-and-their-in-vitro-antiprotozoal-activity
#7
Julia B Althaus, Claudine Malyszek, Marcel Kaiser, Reto Brun, Thomas J Schmidt
In our ongoing study to evaluate the antiprotozoal activity of alkamides from Asteraceae, a dichloromethane extract from the roots of Anacycluspyrethrum L. showed a moderate in vitro activity against the NF54 strain of Plasmodium falciparum and against Leishmaniadonovani (amastigotes, MHOM/ET/67/L82 strain). Seven pure alkamides and a mixture of two further alkamides were isolated by column chromatography followed by preparative high performance liquid chromatography. The alkamides were identified by mass- and NMR-spectroscopic methods as tetradeca-2E,4E-dien-8,10-diynoic acid isobutylamide (anacycline, 1), deca-2E,4E-dienoic acid isobutylamide (pellitorine, 2), deca-2E,4E,9-trienoic acid isobutylamide (3), deca-2E,4E-dienoic acid 2-phenylethylamide (4), undeca-2E,4E-dien-8,10-diynoic acid isopentylamide (5), tetradeca-2E,4E,12Z-trien-8,10-diynoic acid isobutylamide (6), and dodeca-2E,4E-dien acid 4-hydroxy-2-phenylethylamide (7)...
May 12, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28493961/aldosterone-signaling-regulates-the-over-expression-of-claudin-4-and-8-at-the-distal-nephron-from-type-1-diabetic-rats
#8
Eduardo Molina-Jijón, Rafael Rodríguez-Muñoz, Ricardo González-Ramírez, Carmen Namorado-Tónix, José Pedraza-Chaverri, Jose L Reyes
Hyperglycemia in diabetes alters tight junction (TJ) proteins in the kidney. We evaluated the participation of aldosterone (ALD), and the effect of spironolactone (SPL), a mineralocorticoid receptor antagonist, on the expressions of claudin-2, -4, -5 and -8, and occludin in glomeruli, proximal and distal tubules isolated from diabetic rats. Type 1 diabetes was induced in female Wistar rats by a single tail vein injection of streptozotocin (STZ), and SPL was administrated daily by gavage, from days 3-21. Twenty-one days after STZ injection the rats were sacrificed...
2017: PloS One
https://www.readbyqxmd.com/read/28493289/intestinal-epithelial-claudins-expression-and-regulation-in-homeostasis-and-inflammation
#9
REVIEW
Vicky Garcia-Hernandez, Miguel Quiros, Asma Nusrat
The intestinal epithelium forms a highly dynamic and selective barrier that controls absorption of fluid and solutes while restricting pathogen access to underlying tissues. Barrier properties are achieved by intercellular junctions that include an apical tight junction (TJ) and subjacent adherens junctions and desmosomes. The TJ tetraspan claudin proteins form pores between epithelial cells to control paracellular fluid and ion movement. In addition to regulation of barrier function, claudin family members control epithelial homeostasis and are expressed in a spatiotemporal manner in the intestinal crypt-luminal axis...
May 10, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28488774/fscn1-is-upregulated-by-snai2-and-promotes-epithelial-to-mesenchymal-transition-in-head-and-necksquamous-cell-carcinoma
#10
Lei Wang, YaoPeng Jia, ZengYu Jiang, Wei Gao, BinQuan Wang
In this study, we investigated whether there is any association between the expression of FSCN1 and SNAI2and the possible underlying mechanisms in head and neck squamous cell carcinoma (HNSC). In addition, we also investigated whether FSCN1 modulates epithelial to mesenchymal transition (EMT) in HNSC cells. Microarray data of dysregulated genes in HNSC was searched in GEO datasets. The association between FSCN1 expression and the 5-year/10-year overall survival (OS), as well as the correlation between the expression of FSCN1 and SOX2, MYBL2, SNAI2, STAT1 and SOX4 were analyzed based on data in TCGA HNSC cohort (TCGA-HNSC)...
May 10, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28488764/astrocytes-contribute-to-a%C3%AE-induced-blood-brain-barrier-damage-through-activation-of-endothelial-mmp9
#11
Simona Federica Spampinato, Sara Merlo, Yasuteru Sano, Takashi Kanda, Maria Angela Sortino
The blood brain barrier (BBB) plays an important role in the maintenance of the brain homeostasis, and its proper functions are warranted by the interplay between different cellular components (endothelial cells, astrocytes and pericytes). BBB dysfunctions in pathological conditions, and in particular in Alzheimer's disease, have been documented. Here, using an in vitro BBB model, the interaction between endothelial cells and astrocytes exposed to Aβ1-42 was investigated. Human endothelial cells, cultured in monolayer or co-cultured with astrocytes, were exposed to Aβ1-42 (2 μM for 18 hours)...
May 10, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28487998/effects-of-dietary-l-tryptophan-supplementation-on-intestinal-response-to-chronic-unpredictable-stress-in-broilers
#12
Yunshuang Yue, Yuming Guo, Ying Yang
Stress has been recognized as a critical risk factor for gastrointestinal diseases in both humans and animals. However, nutritional strategies to attenuate stress-induced intestinal barrier function and underlying mechanisms remain largely unknown. This study tested the hypothesis that L-tryptophan enhanced intestinal barrier function by regulating mucosal serotonin metabolism in chronic unpredictable stress-exposed broilers. One-day-old male broilers (Arbor Acres) were fed a basal diet supplemented with or without L-tryptophan in the absence or presence of chronic unpredictable stress...
May 9, 2017: Amino Acids
https://www.readbyqxmd.com/read/28485323/rhubarb-monomers-protect-intestinal-mucosal-barrier-in-sepsis-via-junction-proteins
#13
Lyu Wang, Yun-Liang Cui, Zhe Zhang, Zhao-Fen Lin, De-Chang Chen
BACKGROUND: Leakage of the intestinal mucosal barrier may cause translocation of bacteria, then leading to multiorgan failure. This study hypothesized that rhubarb monomers might protect the gut mucosal barrier in sepsis through junction proteins. METHODS: Healthy male Sprague-Dawley rats (weighing 230-250 g) under anesthesia and sedation were subjected to cecal ligation and perforation (CLP). After surgical preparation, rats were randomly assigned to eight groups (n = 6 or 8 each group): sham group (Group A: normal saline gavage); sepsis group (Group B: normal saline gavage); Group C (intraperitoneally, dexamethasone 0...
May 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28485155/nfat5-inhibits-invasion-and-promotes-apoptosis-in-hepatocellular-carcinoma-associated-with-osmolality
#14
X Qin, Y Wang, J Li, Y Xiao, Z Liu
Hepatocellular carcinoma (HCC) is one of the most difficult cancer disease for diagnosis and treatment, with a low survival rate and high recurrence rate and mortality. Nuclear factor of activated T-cells 5 (NFAT5) is mediated by osmolality and proved to be a carcinogenic gene in some tumor. However in our study we considered NFAT5 as tumor suppressor of HCC. RT-qPCR was performed for NFAT5 expression in tumor tissues. NaCl was applied to make hyperosmotic treatment. We knockdowned and overexpressed NFAT5 to investigate its role in HCC...
May 9, 2017: Neoplasma
https://www.readbyqxmd.com/read/28485152/design-and-synthesis-of-a-caged-carboxylic-acid-with-a-donor-%C3%AF-donor-coumarin-structure-one-photon-and-two-photon-uncaging-reactions-using-visible-and-near-infrared-lights
#15
Youhei Chitose, Manabu Abe, Ko Furukawa, Jhe-Yi Lin, Tzu-Chau Lin, Claudine Katan
A caged carboxylic acid with a novel two-photon (TP)-responsive donor-π-donor coumarin backbone with a quadrupolar nature was designed and synthesized in this study. The newly synthesized coumarin derivative showed a strong one-photon (OP) absorption band (ε ≈ 29000 cm(-1) M(-1)) in the visible region (>∼400 nm). Time-dependent density functional theory calculations predicted a sizable TP absorption cross-section with a maximum at ∼650 nm significantly lager than that related to the OP absorption band...
May 9, 2017: Organic Letters
https://www.readbyqxmd.com/read/28483761/a-phase-1b-study-of-isatuximab-plus-lenalidomide-and-dexamethasone-for-relapsed-refractory-multiple-myeloma
#16
Thomas Martin, Rachid Baz, Don M Benson, Nikoletta Lendvai, Jeffrey Wolf, Pamela Munster, Alexander M Lesokhin, Claudine Wack, Eric Charpentier, Frank Campana, Ravi Vij
This phase 1b, open-label, dose-escalation study (NCT01749969) assessed the safety, efficacy, and pharmacokinetics of anti-CD38 monoclonal antibody isatuximab given in two schedules (3, 5, 10 mg/kg every other week [Q2W] or 10, 20 mg/kg weekly for 4 weeks then Q2W thereafter [QW/Q2W]), in combination with lenalidomide 25 mg (Days 1-21) and dexamethasone 40 mg (weekly), in patients with relapsed/refractory multiple myeloma (RRMM). Patients received 28-day treatment cycles; the primary objective was to determine the maximum tolerated dose (MTD) of isatuximab with lenalidomide and dexamethasone...
May 8, 2017: Blood
https://www.readbyqxmd.com/read/28481779/claudin-4-expression-is-associated-with-survival-in-ovarian-cancer-but-not-with-chemotherapy-response
#17
Laura Martín de la Fuente, Susanne Malander, Linda Hartman, Jenny-Maria Jönsson, Anna Ebbesson, Mef Nilbert, Anna Måsbäck, Ingrid Hedenfalk
The tight junction protein claudin-4 has been reported to be overexpressed in advanced ovarian cancer. We investigated the prognostic significance of claudin-4 overexpression and whether claudin-4 expression could predict platinum response in primary ovarian carcinoma (OC). Claudin-4 expression was evaluated by immunohistochemistry in a tissue microarray of 140 OCs. Multivariable Cox-regression models were used to assess the effect of claudin-4 overexpression on progression-free survival and overall survival (OS)...
May 5, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28476740/initiation-of-trastuzumab-by-women-younger-than-64-years-for-adjuvant-treatment-of-stage-i-iii-breast-cancer
#18
Huei-Ting Tsai, Claudine Isaacs, Filipa C Lynce, Suzanne C O'Neill, Chunfu Liu, Marc D Schwartz, Nandini Selvam, Yingjun Zhou, Arnold L Potosky
Purpose: Studies have reported disparities by age and race in the initiation of adjuvant trastuzumab for the initial treatment of older women with early-stage breast cancer, but less is known about its initiation in younger patients. Therefore, we assessed temporal trends and clinical and demographic factors associated with trastuzumab initiation in a large, population-based cohort of patients aged <64 years in 5 states. Methods: Using a cancer registry and claims-linked data set of 13,398 women with incident invasive breast cancer from 2006 to 2011, we identified 934 patients aged <64 years with HER2-positive stage I-III breast cancer...
May 2017: Journal of the National Comprehensive Cancer Network: JNCCN
https://www.readbyqxmd.com/read/28475514/recurrent-cutaneous-angiosarcoma-of-the-scalp-with-aberrant-expression-of-s100-a-case-report
#19
Laura Macías-García, Concepción Lara-Bohorquez, Enrique Jorquera-Barquero, Juan J Ríos-Martín
Angiosarcoma (AS) is a malignant mesenchymal neoplasm of endothelial origin with a predominantly lymphatic immunophenotype, which accounts for less than 1% of all sarcomas. Cutaneous AS of the scalp is associated with high rates of local recurrence and a poor prognosis. Histologically, poorly differentiated AS often comprises solid epithelioid cells, although rare variants involving spindle cells have been reported; diagnosis requires immunohistochemical analysis using vascular cell markers. We report on a cutaneous spindle-cell AS of the scalp in a female patient; key features included spontaneous regression after biopsy, local recurrence 2 years later, and aberrant nuclear staining for S100 protein in an area of the tumor not expressing CD34 or D2-40...
April 25, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28460958/efficiency-of-population-dependent-sulfite-against-brettanomyces-bruxellensis-in-red-wine
#20
Cédric Longin, Claudine Degueurce, Frédérique Julliat, Michèle Guilloux-Benatier, Sandrine Rousseaux, Hervé Alexandre
Brettanomyces bruxellensis is considered as a spoilage yeast encountered mainly in red wine. It is able to reduce vinylphenols from phenolic acids to ethylphenols. These volatiles are responsible for the phenolic "Brett character" described as animal, farm, horse sweat and animal leather odors. Other molecules are responsible for organoleptic deviations described as "mousiness taint". SO2 is the product most often used by winemakers to prevent B. bruxellensis growth. Usually, the recommended molecular dose of SO2 (active SO2, mSO2) is highly variable, from 0...
November 2016: Food Research International
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