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https://www.readbyqxmd.com/read/27909732/early-vegf-inhibition-attenuates-blood-brain-barrier-disruption-in-ischemic-rat-brains-by-regulating-the-expression-of-mmps
#1
Hai-Tao Zhang, Ping Zhang, Yi Gao, Chen-Long Li, Hong-Jun Wang, Ling-Chao Chen, Yan Feng, Rui-Yan Li, Yong-Li Li, Chuan-Lu Jiang
Vascular endothelial growth factor (VEGF) inhibition has been demonstrated to be an effective strategy in preserving the integrity of the blood-brain barrier (BBB) in patients with acute ischemic stroke. Loss of the BBB is the key event associated with morbidity and mortality in these patients. However, the underlying mechanisms remain poorly understood. In the present study, the effects of VEGF inhibition and the possible mechanism that underlies acute cerebral ischemia in rats was investigated. Following the induction of transient middle cerebral artery occlusion for a 90‑min period, either an anti‑VEGF neutralizing antibody (RB‑222; 5 or 10 µg), or IgG (control), was administered by intracerebroventricular injection at 1 h following reperfusion...
November 29, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27893847/a2a-adenosine-receptor-antagonism-reverts-the-blood-brain-barrier-dysfunction-induced-by-sleep-restriction
#2
Gabriela Hurtado-Alvarado, Emilio Domínguez-Salazar, Javier Velázquez-Moctezuma, Beatriz Gómez-González
Chronic sleep restriction induces blood-brain barrier disruption and increases pro-inflammatory mediators in rodents. Those inflammatory mediators may modulate the blood-brain barrier and constitute a link between sleep loss and blood-brain barrier physiology. We propose that adenosine action on its A2A receptor may be modulating the blood-brain barrier dynamics in sleep-restricted rats. We administrated a selective A2A adenosine receptor antagonist (SCH58261) in sleep-restricted rats at the 10th day of sleep restriction and evaluated the blood-brain barrier permeability to dextrans coupled to fluorescein (FITC-dextrans) and Evans blue...
2016: PloS One
https://www.readbyqxmd.com/read/27888130/protective-role-of-phenylalanine-on-the-ros-induced-oxidative-damage-apoptosis-and-tight-junction-damage-via-nrf2-tor-and-nf-%C3%AE%C2%BAb-signalling-molecules-in-the-gill-of-fish
#3
Lin Feng, Wen Li, Yang Liu, Wei-Dan Jiang, Sheng-Yao Kuang, Pei Wu, Jun Jiang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou
This study explored the possible preventive effects of dietary phenylalanine (Phe) on antioxidant responses, apoptosis and tight junction protein transcription in the gills of young grass carp (Ctenopharyngodon idella). Fish were fed six different experimental diets containing graded levels of Phe (3.4-16.8 g kg(-1)) for 8 weeks. The results showed that Phe deficiency induced protein oxidation and lipid peroxidation by decreasing the glutathione content and the activities and mRNA levels of Cu/Zn superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) in fish gill (P < 0...
November 23, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27883241/neutrophil-autophagy-and-extracellular-dna-traps-contribute-to-airway-inflammation-in-severe-asthma
#4
Duy Le Pham, Ga-Young Ban, Seung-Hyun Kim, Yoo Seob Shin, Young-Min Ye, Yong-Joon Chwae, Hae-Sim Park
BACKGROUND: Autophagy and neutrophil extracellular DNA traps (NETs) are implicated in asthma; however, their roles in asthma pathogenesis have not been elucidated. OBJECTIVES: We compared autophagy and NET production levels from peripheral blood neutrophils (PBNs) of patients with severe asthma (SA) and non-severe asthma (NSA). Additionally, we investigated the inflammatory effects of NETs on human airway epithelial cells (AECs) and peripheral blood eosinophils (PBEs)...
November 24, 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27880936/dietary-grape-seed-proanthocyanidins-gsps-improve-weaned-intestinal-microbiota-and-mucosal-barrier-using-a-piglet-model
#5
Meng Han, Peixia Song, Chang Huang, Arash Rezaei, Shabnam Farrar, Michael A Brown, Xi Ma
Proanthocyanidins have been suggested as an effective antibiotic alternative, however their mechanisms are still unknown. The present study investigated the effects of grape seed proanthocyanidins on gut microbiota and mucosal barrier using a weaned piglet model in comparison with colistin. Piglets weaned at 28 day were randomly assigned to four groups treated with a control ration, or supplemented with 250 mg/kg proanthocyanidins, kitasamycin/colistin, or 250 mg/kg proanthocyanidins and half-dose antibiotics, respectively...
November 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27879681/kuwanon-g-preserves-lps-induced-disruption-of-gut-epithelial-barrier-in-vitro
#6
Hengli Guo, Youhua Xu, Wei Huang, Hua Zhou, Zhaoguang Zheng, Yonghua Zhao, Bao He, Tingting Zhu, Shanshan Tang, Quan Zhu
Defects in the gut epithelial barrier have now been recognized to be responsible for diabetic endotoxemia. In everyday life, Mulberry leaf tea is widely used in Asian nations due to its proposed benefits to health and control of diabetes. Evidence indicates the potential role of Kuwanon G (KWG), a component from Morus alba L., on blocking the gut epithelial barrier. In lipopolysaccharides (LPS)-damaged Caco-2 cells, it was found that KWG increased the viability of cells in a concentration-dependent manner. KWG administration significantly elevated the anti-oxidant abilities via increasing ratio of superoxidase dismutase (SOD)/malondialdehyde (MDA) and decreasing reactive oxygen species (ROS) within the cells...
November 22, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27876210/conditioned-medium-from-ls-174t-goblet-cells-treated-with-oxyresveratrol-strengthens-tight-junctions-in-caco-2-cells
#7
Dahyun Hwang, HyunA Jo, Seonwook Hwang, Jeong-Keun Kim, In-Ho Kim, Young-Hee Lim
BACKGROUND: Strengthening of intestinal tight junctions provides an effective barrier from the external environment. Goblet cell-derived trefoil factor 3 (TFF3) increases transepithelial resistance by upregulating the expression of tight junction proteins. Oxyresveratrol (OXY) is a hydroxyl-substituted stilbene found in the roots, leaves, stems, and fruit of many plants and known to have various biological activities. In this study, we investigated the strengthening effect of OXY on intestinal tight junctions through stimulation of TFF production in goblet cells...
November 18, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27868153/carnosine-attenuates-brain-oxidative-stress-and-apoptosis-after-intracerebral-hemorrhage-in-rats
#8
Rong-Xia Xie, Da-Wei Li, Xi-Chang Liu, Ming-Feng Yang, Jie Fang, Bao-Liang Sun, Zong-Yong Zhang, Xiao-Yi Yang
Carnosine, an endogenous dipeptide (β-alanyl-L-histidine), exerts multiple neuroprotective properties, but its role in intracerebral hemorrhage (ICH) remains unclear. This study investigates the effect of Carnosine on brain injury using the rat ICH model, which is established by type IV collagenase caudatum infusion. The results indicate that intraperitoneal administration of Carnosine (1000 mg/kg) significantly attenuates brain edema, blood-brain barrier (BBB) disruption, oxidative stress, microglia activation and neuronal apoptosis of perihematoma at 72 h following ICH in rats models, as convinced by preventing the disruption of tight junction protein ZO-1, occludin and claudin-5, followed by the decrease of ROS, MDA, 3-NT, 8-OHDG level and the increase of GSH-Px and SOD activity, then followed by the decline of Iba-1, ED-1, active caspase-3 and TUNEL positive cells and the decrease of IL-1β, IL-6, TNF-α, active caspase-3 and cytochrome c level...
November 21, 2016: Neurochemical Research
https://www.readbyqxmd.com/read/27846191/noncoding-transcribed-ultraconserved-region-t-ucr-uc-261-participates-in-intestinal-mucosa-barrier-damage-in-crohn-s-disease
#9
Xiao Xian Qian, Jiang Chen Peng, An Tao Xu, Di Zhao, Yu Qi Qiao, Tian Rong Wang, Jun Shen, Zhi Hua Ran
BACKGROUND: To date, 481 ultraconserved regions (UCRs) have been discovered in human genome. We aimed to investigate the transcribed UCR (T-UCR) characteristics in Crohn's disease (CD ) and determine whether T-UCR uc.261 participated in intestinal mucosa barrier damage. METHODS: T-UCRs were screened in active CD mucosa using the Arraystar Human T-UCR Microarray and validated with quantitative real-time reverse transcription PCR, together with tight junction proteins (TJPs) including junctional adhesion molecule-A, occludin, claudin-1, and zonula occluden-1...
December 2016: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/27834208/expanding-the-host-range-of-hepatitis-c-virus-through-viral-adaptation
#10
Markus von Schaewen, Marcus Dorner, Kathrin Hueging, Lander Foquet, Sherif Gerges, Gabriela Hrebikova, Brigitte Heller, Julia Bitzegeio, Juliane Doerrbecker, Joshua A Horwitz, Gisa Gerold, Sebastian Suerbaum, Charles M Rice, Philip Meuleman, Thomas Pietschmann, Alexander Ploss
: Hepatitis C virus (HCV) species tropism is incompletely understood. We have previously shown that at the level of entry, human CD81 and occludin (OCLN) comprise the minimal set of human factors needed for viral uptake into murine cells. As an alternative approach to genetic humanization, species barriers can be overcome by adapting HCV to use the murine orthologues of these entry factors. We previously generated a murine tropic HCV (mtHCV or Jc1/mCD81) strain harboring three mutations within the viral envelope proteins that allowed productive entry into mouse cell lines...
November 8, 2016: MBio
https://www.readbyqxmd.com/read/27824650/vsl-3-probiotic-stimulates-t-cell-protein-tyrosine-phosphatase-mediated-recovery-of-ifn-%C3%AE-induced-intestinal-epithelial-barrier-defects
#11
Moorthy Krishnan, Harrison M Penrose, Nilay N Shah, Ronald R Marchelletta, Declan F McCole
BACKGROUND: VSL#3 is a probiotic compound that has been used in the treatment of inflammatory bowel disease. T-cell protein tyrosine phosphatase (TCPTP) is the protein product of the inflammatory bowel disease candidate gene, PTPN2, and we have previously shown that it protects epithelial barrier function. The aim of this study was to investigate whether VSL#3 improves intestinal epithelial barrier function against the effects of the inflammatory bowel disease-associated proinflammatory cytokine, interferon-gamma (IFN-γ) through activation of TCPTP...
December 2016: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/27824273/anti-inflammaging-effects-of-lactobacillus-brevis-ow38-in-aged-mice
#12
J-J Jeong, K A Kim, Y-J Hwang, M J Han, D-H Kim
In the present study, lactic acid bacteria (LAB) strains were collected from kimchi and were screened to isolate strains that inhibit lipopolysaccharide (LPS) production by Escherichia coli and p16 expression and nuclear factor-kappa B (NF-κB) activation in LPS-stimulated macrophages. Oral administration of Lactobacillus brevis OW38 (1×10(9) cfu/mouse) to aged mice (male, 18 months old) for 8 weeks reduced the LPS level in colon fluid and blood. In addition, OW38 treatment also reduced the ratio of Firmicutes or Proteobacteria to Bacteroidetes, which was significantly higher in aged mice than in young mice...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27823626/plant-derived-triterpene-celastrol-ameliorates-oxygen-glucose-deprivation-induced-disruption-of-endothelial-barrier-assembly-via-inducing-tight-junction-proteins
#13
Dan Luo, Jia Zhao, Jianhui Rong
BACKGROUND: The integrity and functions of blood-brain barrier (BBB) are regulated by the expression and organization of tight junction proteins. OBJECTIVE: The present study was designed to explore whether plant-derived triterpenoid celastrol could regulate tight junction integrity in murine brain endothelial bEnd3 cells. METHODS: We disrupted the tight junctions between endothelial bEnd3 cells by oxygen glucose deprivation (OGD). We investigated the effects of celastrol on the permeability of endothelial monolayers by measuring transepithelial electrical resistance (TEER)...
December 1, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27818230/effect-of-ppar-%C3%AE-%C3%AE-agonist-gw0742-treatment-in-the-acute-phase-response-and-blood-brain-barrier-permeability-following-brain-injury
#14
Khouloud Chehaibi, Laura le Maire, Sarah Bradoni, Joan Carles Escola, Francisco Blanco-Vaca, Mohamed Naceur Slimane
The systemic response to ischemic stroke is associated with the hepatic acute phase response (APR) that modulates leukocytes recruitment to the injured brain. The inappropriate recruitment of leukocytes to the brain parenchyma can result in blood-brain barrier (BBB) breakdown. Emerging data suggest that peroxisome proliferator-activated receptor beta/delta (PPAR-β/δ) activation has a potential neuroprotective role in ischemic stroke. However, mechanisms of PPAR-β/δ mediated protection in ischemic insults remain unclear...
October 18, 2016: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/27818224/perfluorooctane-sulfonate-pfos-disrupts-blood-testis-barrier-by-down-regulating-junction-proteins-via-p38-mapk-atf2-mmp9-signaling-pathway
#15
Lianglin Qiu, Yingyun Qian, Zhenzhen Liu, Chao Wang, Jianhua Qu, Xiaoke Wang, Shoulin Wang
Perfluorooctane sulfonate (PFOS), an ubiquitous environmental pollutant, has been associated with male reproductive disorders. However, the underlying mechanisms are not yet fully understood. In this study, in vivo and in vitro models were used to explore the effects of PFOS on blood-testis barrier (BTB) and related molecular mechanisms. First, male ICR mice were orally administrated PFOS (0.5-10mg/kg/bw) for 4 weeks. Bodyweight, sperm count, BTB integrity and the expression of proteins including p38 mitogen-activated protein kinase (MAPK), activating transcription factor 2 (ATF2), matrix metalloproteinase 9 (MMP9), tissue inhibitor of metalloproteinase 1(TIMP1) and BTB related junction proteins were evaluated...
December 12, 2016: Toxicology
https://www.readbyqxmd.com/read/27816443/lucidone-promotes-the-cutaneous-wound-healing-process-via-activation-of-the-pi3k-akt-wnt-%C3%AE-catenin-and-nf-%C3%AE%C2%BAb-signaling-pathways
#16
Hsin-Ling Yang, Yu-Cheng Tsai, Mallikarjuna Korivi, Chia-Ting Chang, You-Cheng Hseu
Lucidone, which comprises a naturally occurring cyclopentenedione, has been investigated for its in vitro and in vivo wound healing properties, and the underlying molecular signaling cascades in the wound healing mechanism have been elucidated. We demonstrated the cell-/dose-specific responses of lucidone (0.5-8μM) on proliferation and migration/invasion of keratinocyte HaCaT and fibroblast Hs68 cells. In keratinocytes, lucidone-induced nuclear translocation of β-catenin was accompanied by increased transcriptional target genes, including c-Myc and cyclin-D1, through GSK3β-dependent pathway...
November 2, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27815408/epha2-proteomics-in-human-keratinocytes-reveals-a-novel-association-with-afadin-and-epidermal-tight-junctions
#17
Bethany E Perez White, Rosa Ventrella, Nihal Kaplan, Calvin J Cable, Paul M Thomas, Spiro Getsios
EphA2 is a receptor tyrosine kinase that helps maintain epidermal tissue homeostasis. A BioID approach was used to identify proteins in close proximity to EphA2 in primary human keratinocytes and 3-D reconstituted human epidermis cultures to map a putative protein interaction network for this membrane receptor that exhibits a polarized distribution in stratified epithelia. While a subset of known EphA2 interactors were identified in the BioID screen, >97% were uniquely detected in keratinocytes with over 50% of these vicinal proteins only present in 3-D human epidermal culture...
November 4, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27815259/aging-exacerbates-acute-lung-injury-induced-changes-of-the-air-blood-barrier-lung-function-and-inflammation-in-the-mouse
#18
Katharina Maria Kling, Elena Lopez-Rodriguez, Christiane Pfarrer, Christian Mühlfeld, Christina Brandenberger
Acute lung injury (ALI) is characterized by hypoxemia, enhanced permeability of the air-blood barrier and pulmonary edema. Particularly in the elderly ALI is associated with increased morbidity and mortality. The reasons for this, however, are poorly understood. We hypothesized that age-related changes in pulmonary structure, function and inflammation, lead to a worse prognosis in ALI. ALI was induced in young (2 months) and old (18 months) male C57BL/6 mice by intranasal application of 2.5 mg lipopolysaccharide (LPS)/kg body weight or saline (control mice)...
November 4, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27815150/solnatide-demonstrates-profound-therapeutic-activity-in-a-rat-model-of-pulmonary-edema-induced-by-acute-hypobaric-hypoxia-and-exercise
#19
Qiquan Zhou, Dong Wang, Yunsheng Liu, Xiaohong Yang, Rudolf Lucas, Bernhard Fischer
BACKGROUND: The synthetic peptide solnatide is a novel pharmacological agent that reduces extravascular lung water, blunts reactive oxygen species production and improves lung function due to its ability to directly activate the epithelial sodium channel ENaC. We aimed to investigate the effect of solnatide in pulmonary edema induced by acute hypobaric hypoxia and exercise in rats, which is considered a model for high altitude pulmonary edema. METHODS: Sprague-Dawley rats were assigned to low altitude control and eight treatment groups...
November 1, 2016: Chest
https://www.readbyqxmd.com/read/27806407/tangeretin-inhibits-il-12-expression-and-nf-%C3%AE%C2%BAb-activation-in-dendritic-cells-and-attenuates-colitis-in-mice
#20
Su-Hyeon Eun, Je-Te Woo, Dong-Hyun Kim
In the preliminary study, tangeretin (5,6,7,8,4'-pentamethoxy flavone), a major constituent of the pericarp of Citrus sp., inhibited TNF-α, IL-12, and IL-23 expression and nuclear factor kappa-B activation in lipopolysaccharide-stimulated dendritic cells; however, it did not affect IL-10 expression. Furthermore, tangeretin (5, 10, and 20 µM) suppressed the activation and translocation of nuclear factor kappa-B (p65) into the nuclei in vitro by inhibiting the binding of lipopolysaccharide on dendritic cells...
November 2, 2016: Planta Medica
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