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https://www.readbyqxmd.com/read/29803674/expression-of-zo1-vimentin-pan-cadherin-and-agtr1-in-tanycyte-like-cells-of-the-sulcus-medianus-organum
#1
Muthanna Al-Kaabi, Fadhil Hussam, Sarmad Al-Marsoummi, Ali Al-Anbaki, Anam Al-Salihi, Hayder Al-Aubaidy
Tanycytes are a specialized ependymal lining of brain ventricles with exceptional features of having long basal processes and junctional complexes between cell bodies. These tanycytes are present at the regions of circumventricular organs (CVOs) which possess common morphological and functional features enabling them to be described as the brain windows where the barrier systems have special properties. Previous studies detailed seven of these CVOs but little information is available regarding another putative site at the rostral part of the median sulcus of the 4th ventricle, or the sulcus medianus organum (SMO)...
May 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29659957/dietary-fermentable-fibers-attenuate-chronic-kidney-disease-in-mice-by-protecting-the-intestinal-barrier
#2
Tran Van Hung, Takuya Suzuki
Background: Chronic kidney disease (CKD) is a worldwide health problem. Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development. Objective: We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice...
April 1, 2018: Journal of Nutrition
https://www.readbyqxmd.com/read/29538713/allium-hookeri-supplementation-improves-intestinal-immune-response-against-necrotic-enteritis-in-young-broiler-chickens
#3
Y S Lee, S H Lee, U D Gadde, S T Oh, S J Lee, H S Lillehoj
Three hundred birds (1 day old) were randomly assigned to 6 groups (n = 50 birds/treatment) and fed a basal diet (control) or basal diet supplemented with Allium hookeri (AH) root (1 or 3%). At day 14, half of the birds in each group were orally challenged with E. maxima 41A (1 × 104 cells/chicken), followed by C. perfringens infection (1 × 109 cfu/chicken) on day 18. Necrotic enteritis (NE)-associated infections and intestinal immune response were assessed by average body weight gain, lesion score, and oocyst shedding...
June 1, 2018: Poultry Science
https://www.readbyqxmd.com/read/29452218/tnf%C3%AE-disrupts-blood-brain-barrier-integrity-to-maintain-prolonged-depressive-like-behavior-in-mice
#4
Yuyan Cheng, Sachi Desse, Ana Martinez, Ryan J Worthen, Richard S Jope, Eleonore Beurel
Recovery from major depressive disorder is difficult, particularly in patients who are refractory to antidepressant treatments. To examine factors that regulate recovery, we developed a prolonged learned helplessness depression model in mice. After the induction of learned helplessness, mice were separated into groups that recovered or did not recover within 4 weeks. Comparisons were made between groups in hippocampal proteins, inflammatory cytokines, and blood brain barrier (BBB) permeability. Compared with mice that recovered and control mice, non-recovered mice displaying prolonged learned helplessness had greater hippocampal activation of glycogen synthase kinase-3 (GSK3), higher levels of tumor necrosis factor-α (TNFα), interleukin-17A, and interleukin-23, increased permeability of the blood brain barrier (BBB), and lower levels of the BBB tight junction proteins occludin, ZO1, and claudin-5...
March 2018: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/29286098/luteolin-inhibits-pancreatitis%C3%A2-induced-acinar%C3%A2-ductal-metaplasia-proliferation-and-epithelial%C3%A2-mesenchymal-transition-of-acinar-cells
#5
Xince Huang, Pravin Avinash Bhugul, Gang Fan, Tingting Ye, Shihao Huang, Shengjie Dai, Bicheng Chen, Mengtao Zhou
Luteolin, a flavone, has been demonstrated to have anti‑cancer properties. In the current study, the effects of luteolin on certain carcinogenesis‑associated changes induced by pancreatitis, which are significant risk factors for pancreatic cancer, were investigated. Male six‑week‑old C57BL6 mice used in the current study were divided into three groups; the control group, acute pancreatitis group and luteolin group. Intra‑peritoneal injection of cearulein was performed in the acute pancreatitis group and luteolin group to induce acute pancreatitis whereas the luteolin group received intra‑peritoneal injection of luteolin...
March 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29221725/protective-effects-of-polyphenol-rich-infusions-from-carob-ceratonia-siliqua-leaves-and-cladodes-of-opuntia-ficus-indica-against-inflammation-associated-with-diet-induced-obesity-and-dss-induced-colitis-in-swiss-mice
#6
Ikram Aboura, Abdelhafid Nani, Meriem Belarbi, Babar Murtaza, Aurélie Fluckiger, Adélie Dumont, Chahid Benammar, Moufida Saidani Tounsi, François Ghiringhelli, Mickaël Rialland, Naim Akhtar Khan, Aziz Hichami
In the present study, we have investigated the effects of polyphenol-rich infusions from carob leaves and OFI-cladodes on inflammation associated with obesity and dextran sulfate sodium (DSS)-induced ulcerative colitis in Swiss mice. In vitro studies revealed that aqueous extracts of carob leaves and OFI-cladodes exhibited anti-inflammatory properties marked by the inhibition of IL-6, TNF-α and nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells concomitant with NF-κβ nucleus translocation inhibition...
December 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29200841/mapk-foxa2-mediated-cigarette-smoke-induced-squamous-metaplasia-of-bronchial-epithelial-cells
#7
Chunling Du, Jinchang Lu, Lei Zhou, Bo Wu, Feng Zhou, Liang Gu, Donghui Xu, Yingxin Sun
Objective: To explore the effect of cigarette smoke (CS) on the development of squamous metaplasia in human airway epithelial cells and the role of MAPK- and FoxA2-signaling pathways in the process. Materials and methods: In an in vitro study, we treated the bronchial epithelial cell line BEAS2B with CS extract, followed by treatment with the ERK inhibitor U0126, the JNK inhibitor SP600125, or the p38 inhibitor SB203580. In vivo, we used a CS-induced rat model. After treatment with CS with or without MAPK inhibitors for 90 days, lung tissues were harvested...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/29032508/development-and-characterization-of-2-dimensional-culture-for-buffalo-intestinal-cells
#8
Nidhi Chaudhary, Himanshu Agrawal, Mamta Pandey, Suneel Onteru, Dheer Singh
Small intestinal epithelial cells (IEC) play a major role in the absorption of nutrients and toxins. Due to the similarity of genome-wide single copy protein orthologues between cattle and human, establishment of ruminant's primary small IEC culture could be a valuable tool for toxicity studies. Therefore, the current study focused on the development and characterization of buffalo IEC culture, as cattle slaughter is banned in India. The buffalo jejunum fragments were washed consecutively several times in saline, warm phosphate buffered saline (PBS), PBS with 5 mM dithiothreitol, digesting solution and 2% sorbitol in PBS...
February 2018: Cytotechnology
https://www.readbyqxmd.com/read/28822114/glycosylation-of-dentin-matrix-protein-1-is-a-novel-key-element-for-astrocyte-maturation-and-bbb-integrity
#9
Bo Jing, Chunxue Zhang, Xianjun Liu, Liqiang Zhou, Jiping Liu, Yinan Yao, Juehua Yu, Yuteng Weng, Min Pan, Jie Liu, Zuolin Wang, Yao Sun, Yi Eve Sun
The blood-brain barrier (BBB) is a tight boundary formed between endothelial cells and astrocytes, which separates and protects brain from most pathogens as well as neural toxins in circulation. However, detailed molecular players involved in formation of BBB are not completely known. Dentin matrix protein 1 (DMP1)-proteoglycan (PG), which is known to be involved in mineralization of bones and dentin, is also expressed in soft tissues including brain with unknown functions. In the present study, we reported that DMP1-PG was expressed in brain astrocytes and enriched in BBB units...
March 2018: Protein & Cell
https://www.readbyqxmd.com/read/28814507/systematic-gene-tagging-using-crispr-cas9-in-human-stem-cells-to-illuminate-cell-organization
#10
Brock Roberts, Amanda Haupt, Andrew Tucker, Tanya Grancharova, Joy Arakaki, Margaret A Fuqua, Angelique Nelson, Caroline Hookway, Susan A Ludmann, Irina A Mueller, Ruian Yang, Rick Horwitz, Susanne M Rafelski, Ruwanthi N Gunawardane
We present a CRISPR/Cas9 genome-editing strategy to systematically tag endogenous proteins with fluorescent tags in human induced pluripotent stem cells (hiPSC). To date, we have generated multiple hiPSC lines with monoallelic green fluorescent protein tags labeling 10 proteins representing major cellular structures. The tagged proteins include alpha tubulin, beta actin, desmoplakin, fibrillarin, nuclear lamin B1, nonmuscle myosin heavy chain IIB, paxillin, Sec61 beta, tight junction protein ZO1, and Tom20...
October 15, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28794289/differentiation-of-mesenchymal-stem-cells-towards-nephrogenic-lineage-and-their-enhanced-resistance-to-oxygen-peroxide-induced-oxidative-stress
#11
Asima Tayyeb, Naveed Shahzad, Gibran Ali
INTRODUCTION: Mesenchymal stem cells (MSCs) have been publicized to ameliorate kidney injury both in vitro and in vivo. However, very less is known if MSCs can be differentiated towards renal lineages and their further application potential in kidney injuries. MATERIALS AND METHODS: The present study developed a conditioning system of growth factors fibroblast growth factor 2, transforming growth factor-β2, and leukemia inhibitory factor for in vitro differentiation of MSCs isolated from different sources towards nephrogenic lineage...
July 2017: Iranian Journal of Kidney Diseases
https://www.readbyqxmd.com/read/28754554/impairment-of-the-carnitine-organic-cation-transporter-1-ergothioneine-axis-is-mediated-by-intestinal-transporter-dysfunction-in-chronic-kidney-disease
#12
Yasuyuki Shinozaki, Kengo Furuichi, Tadashi Toyama, Shinji Kitajima, Akinori Hara, Yasunori Iwata, Norihiko Sakai, Miho Shimizu, Shuichi Kaneko, Noriyoshi Isozumi, Shushi Nagamori, Yoshikatsu Kanai, Tomoko Sugiura, Yukio Kato, Takashi Wada
Carnitine/organic cation transporter 1 (OCTN1) is a specific transporter of the food-derived antioxidant ergothioneine. Ergothioneine is absorbed by intestinal OCTN1, distributed through the bloodstream, and incorporated into each organ by OCTN1. OCTN1 expression is upregulated in injured tissues, and promotes ergothioneine uptake to reduce further damage caused by oxidative stress. However, the role of the OCTN1-ergothioneine axis in kidney-intestine cross-talk and chronic kidney disease (CKD) progression remains unclear...
December 2017: Kidney International
https://www.readbyqxmd.com/read/28743453/pregnane-x-receptor-deletion-modifies-recognition-memory-and-electroencephalographic-activity
#13
Badreddine Boussadia, Laila Lakhal, Laurence Payrastre, Chaitali Ghosh, Jean-Marc Pascussi, Giuseppe Gangarossa, Nicola Marchi
Nuclear receptors (NR) are emerging as key players in the central nervous system (CNS) with reported implications in physiological and pathophysiological conditions. While a number of NR has been studied, it is unknown whether invalidation of the pregnane xenobiotic receptor (PXR, NR1I2) corresponds to neurological modifications in the adult brain. PXR-/- C57BL/6J and wild-type mice were used to investigate: (i) recognition memory, motor coordination, and anxiety-like behaviors; (ii) longitudinal video-electroencephalographic (EEG) recordings and frequency wave analysis; (iii) neurovascular structures by histological evaluation and expression of the cerebrovascular tight junctions ZO1 and CLDN5...
February 1, 2018: Neuroscience
https://www.readbyqxmd.com/read/28505587/dietary-bacillus-subtilis-based-direct-fed-microbials-alleviate-lps-induced-intestinal-immunological-stress-and-improve-intestinal-barrier-gene-expression-in-commercial-broiler-chickens
#14
Ujvala Deepthi Gadde, Sungtaek Oh, Youngsub Lee, Ellen Davis, Noah Zimmerman, Tom Rehberger, Hyun Soon Lillehoj
This study investigated the effects of Bacillus subtilis-based probiotics on the performance, modulation of host inflammatory responses and intestinal barrier gene expression of broilers subjected to LPS challenge. Chickens were randomly allocated to one of the 3 dietary treatment groups - control, antibiotic, or probiotic. At 14days, half of the chickens in each treatment were injected with LPS (1mg/kg body weight), and the other half injected with sterile PBS. Chickens fed probiotics weighed significantly more than controls at 15days of age, irrespective of immune challenge...
October 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28490769/increased-cerebral-expressions-of-mmps-cldn5-ocln-zo1-and-aqps-are-associated-with-brain-edema-following-fatal-heat-stroke
#15
Yu Du, Jing-Tao Xu, Hong-Nian Jin, Rui Zhao, Dong Zhao, Si-Hao Du, Ye Xue, Xiao-Li Xie, Qi Wang
Human brain samples were collected from 46 autopsy cases, including 23 fatal heat stroke cases and 23 age-matched controls. Nine candidate reference genes (PES1, POLR2A, IPO8, HMBS, SDHA, GAPDH, UBC, B2M, ACTB) were evaluated in the cerebral cortex of 10 forensic autopsy cases (5 heat stroke and 5 controls), using the geNorm module in qBase(plus) software. SDHA, POLR2A, IPO8 and HMBS were identified as the most stable reference genes. Using these validated reference genes, mRNA expressions of Matrix metalloproteinases (MMPs, MMP2 and MMP9), Claudin5 (CLDN5), Occludin (OCLN), Zona occludens protein-1 (ZO1) and Aquaporins (AQPs, AQP1 and AQP4) in the cerebral cortex were examined...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421423/the-effects-of-direct-fed-microbial-supplementation-as-an-alternative-to-antibiotics-on-growth-performance-intestinal-immune-status-and-epithelial-barrier-gene-expression-in-broiler-chickens
#16
U Gadde, S T Oh, Y S Lee, E Davis, N Zimmerman, T Rehberger, Hyun S Lillehoj
The objective of this study was to investigate the effects of Bacillus subtilis-based probiotic supplementation in broiler chicken diets on growth performance, feed efficiency, intestinal cytokine, and tight junction (TJ) protein mRNA expression. Zero-day-old broiler chicks (n = 140) were randomly assigned to one of five dietary treatments: basal diet (CON); basal diet supplemented with either antibiotic bacitracin methylene disalicylate (BMD); or probiotics, namely, B. subtilis strain 1781 (PB1), a combination of B...
December 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28396819/rab11-fip1-phosphorylation-by-mark2-regulates-polarity-in-mdck-cells
#17
Rebecca McRae, Lynne A Lapierre, Elizabeth H Manning, James R Goldenring
MARK2/Par1b/EMK1, a serine/threonine kinase, is required for correct apical/basolateral membrane polarization in epithelial cells. However, the specific substrates mediating MARK2 action are less well understood. We have now found that MARK2 phosphorylates Rab11-FIP1B/C at serine 234 in a consensus site similar to that previously identified in Rab11-FIP2. In MDCK cells undergoing repolarization after a calcium switch, antibodies specific for pS234-Rab11-FIP1 or pS227-Rab11-FIP2 demonstrate that the spatial and temporal activation of Rab11-FIP1 phosphorylation is distinct from that for Rab11-FIP2...
2017: Cellular Logistics
https://www.readbyqxmd.com/read/28387750/bmp6-regulates-proliferation-and-apoptosis-of-human-sertoli-cells-via-smad2-3-and-cyclin-d1-pathway-and-dach1-and-tfap2a-activation
#18
Hong Wang, Qingqing Yuan, Min Sun, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou, Zheng Chen, Chencheng Yao, Jingmei Hou, Ruinan Shen, Qisheng Lin, Wenjie Liu, Ruobing Jia, Zheng Li, Zuping He
Sertoli cells are essential for regulating normal spermatogenesis. However, the mechanisms underlying human Sertoli cell development remain largely elusive. Here we examined the function and signaling pathways of BMP6 in regulating human Sertoli cells. RT-PCR, immunocytochemistry and Western blots revealed that BMP6 and its multiple receptors were expressed in human Sertoli cells. CCK-8 and EDU assays showed that BMP6 promoted the proliferation of Sertoli cells. Conversely, BMP6 siRNAs inhibited the division of these cells...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28376304/origins-of-pdz-binding-specificity-a-computational-and-experimental-study-using-nherf1-and-the-parathyroid-hormone-receptor
#19
Tatyana Mamonova, Qiangmin Zhang, Mintu Chandra, Brett M Collins, Edward Sarfo, Zimei Bu, Kunhong Xiao, Alessandro Bisello, Peter A Friedman
Na+ /H+ exchanger regulatory factor-1 (NHERF1) is a scaffolding protein containing two PSD95/discs large protein/ZO1 (PDZ) domains that modifies the signaling, trafficking, and function of the parathyroid hormone receptor (PTHR), a family B G-protein-coupled receptor. PTHR and NHERF1 bind through a PDZ-ligand-recognition mechanism. We show that PTH elicits phosphorylation of Thr591 in the canonical -ETVM binding motif of PTHR. Conservative substitution of Thr591 with Cys does not affect PTH(1-34)-induced cAMP production or binding of PTHR to NHERF1...
May 23, 2017: Biochemistry
https://www.readbyqxmd.com/read/28251704/ochratoxin-a-cytotoxicity-on-madin-darby-canine-kidney-cells-in-the-presence-of-alpha-tocopherol-effects-on-cell-viability-and-tight-junctions
#20
E Fusi, C Giromini, R Rebucci, L Pinotti, V Caprarulo, F Cheli, F Vitari, C Domeneghini, A Baldi
Ochratoxin A (OTA) is a potent nephrotoxic fungi metabolite that affects animal and human health. At the cellular level, OTA is able to alter functions and viability by several mechanisms of action. Several strategies to counteract its toxicity have been studied. We investigated the role of α-tocopherol in counteracting OTA oxidative damage in Madin-Darby canine kidney (MDCK) cells by pre-incubating the cells for 3 hr with the antioxidant (1 nm, 10 μm) and then adding OTA (0-1.2 μg/ml) for the following 24 hr...
February 2018: Journal of Animal Physiology and Animal Nutrition
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