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https://www.readbyqxmd.com/read/28822114/glycosylation-of-dentin-matrix-protein-1-is-a-novel-key-element-for-astrocyte-maturation-and-bbb-integrity
#1
Bo Jing, Chunxue Zhang, Xianjun Liu, Liqiang Zhou, Jiping Liu, Yinan Yao, Juehua Yu, Yuteng Weng, Min Pan, Jie Liu, Zuolin Wang, Yao Sun, Yi Eve Sun
The blood-brain barrier (BBB) is a tight boundary formed between endothelial cells and astrocytes, which separates and protects brain from most pathogens as well as neural toxins in circulation. However, detailed molecular players involved in formation of BBB are not completely known. Dentin matrix protein 1 (DMP1)-proteoglycan (PG), which is known to be involved in mineralization of bones and dentin, is also expressed in soft tissues including brain with unknown functions. In the present study, we reported that DMP1-PG was expressed in brain astrocytes and enriched in BBB units...
August 18, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28814507/systematic-gene-tagging-using-crispr-cas9-in-human-stem-cells-to-illuminate-cell-organization
#2
Brock Roberts, Amanda Haupt, Andrew Tucker, Tanya Grancharova, Joy Arakaki, Margaret A Fuqua, Angelique Nelson, Caroline Hookway, Susan A Ludmann, Irina A Mueller, Ruian Yang, Rick Horwitz, Susanne M Rafelski, Ruwanthi N Gunawardane
We present a CRISPR/Cas9 genome-editing strategy to systematically tag endogenous proteins with fluorescent tags in human induced pluripotent stem cells (hiPSC). To date, we have generated multiple hiPSC lines with monoallelic green fluorescent protein tags labeling 10 proteins representing major cellular structures. The tagged proteins include alpha tubulin, beta actin, desmoplakin, fibrillarin, nuclear lamin B1, nonmuscle myosin heavy chain IIB, paxillin, Sec61 beta, tight junction protein ZO1, and Tom20...
October 15, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28794289/differentiation-of-mesenchymal-stem-cells-towards-nephrogenic-lineage-and-their-enhanced-resistance-to-oxygen-peroxide-induced-oxidative-stress
#3
Asima Tayyeb, Naveed Shahzad, Gibran Ali
INTRODUCTION: Mesenchymal stem cells (MSCs) have been publicized to ameliorate kidney injury both in vitro and in vivo. However, very less is known if MSCs can be differentiated towards renal lineages and their further application potential in kidney injuries. MATERIALS AND METHODS: The present study developed a conditioning system of growth factors fibroblast growth factor 2, transforming growth factor-β2, and leukemia inhibitory factor for in vitro differentiation of MSCs isolated from different sources towards nephrogenic lineage...
July 2017: Iranian Journal of Kidney Diseases
https://www.readbyqxmd.com/read/28754554/impairment-of-the-carnitine-organic-cation-transporter-1-ergothioneine-axis-is-mediated-by-intestinal-transporter-dysfunction-in-chronic-kidney-disease
#4
Yasuyuki Shinozaki, Kengo Furuichi, Tadashi Toyama, Shinji Kitajima, Akinori Hara, Yasunori Iwata, Norihiko Sakai, Miho Shimizu, Shuichi Kaneko, Noriyoshi Isozumi, Shushi Nagamori, Yoshikatsu Kanai, Tomoko Sugiura, Yukio Kato, Takashi Wada
Carnitine/organic cation transporter 1 (OCTN1) is a specific transporter of the food-derived antioxidant ergothioneine. Ergothioneine is absorbed by intestinal OCTN1, distributed through the bloodstream, and incorporated into each organ by OCTN1. OCTN1 expression is upregulated in injured tissues, and promotes ergothioneine uptake to reduce further damage caused by oxidative stress. However, the role of the OCTN1-ergothioneine axis in kidney-intestine cross-talk and chronic kidney disease (CKD) progression remains unclear...
July 26, 2017: Kidney International
https://www.readbyqxmd.com/read/28743453/pregnane-x-receptor-deletion-modifies-recognition-memory-and-electroencephalographic-activity
#5
Badreddine Boussadia, Laila Lakhal, Laurence Payrastre, Chaitali Ghosh, Jean-Marc Pascussi, Giuseppe Gangarossa, Nicola Marchi
Nuclear receptors (NR) are emerging as key players in the central nervous system (CNS) with reported implications in physiological and pathophysiological conditions. While a number of NR has been studied, it is unknown whether invalidation of the pregnane xenobiotic receptor (PXR, NR1I2) corresponds to neurological modifications in the adult brain. PXR(-/-) C57BL/6J and wild-type mice were used to investigate: (i) recognition memory, motor coordination, and anxiety-like behaviors; (ii) longitudinal video-electroencephalographic (EEG) recordings and frequency wave analysis; (iii) neurovascular structures by histological evaluation and expression of the cerebrovascular tight junctions ZO1 and CLDN5...
July 23, 2017: Neuroscience
https://www.readbyqxmd.com/read/28505587/dietary-bacillus-subtilis-based-direct-fed-microbials-alleviate-lps-induced-intestinal-immunological-stress-and-improve-intestinal-barrier-gene-expression-in-commercial-broiler-chickens
#6
Ujvala Deepthi Gadde, Sungtaek Oh, Youngsub Lee, Ellen Davis, Noah Zimmerman, Tom Rehberger, Hyun Soon Lillehoj
This study investigated the effects of Bacillus subtilis-based probiotics on the performance, modulation of host inflammatory responses and intestinal barrier gene expression of broilers subjected to LPS challenge. Chickens were randomly allocated to one of the 3 dietary treatment groups - control, antibiotic, or probiotic. At 14days, half of the chickens in each treatment were injected with LPS (1mg/kg body weight), and the other half injected with sterile PBS. Chickens fed probiotics weighed significantly more than controls at 15days of age, irrespective of immune challenge...
May 6, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28490769/increased-cerebral-expressions-of-mmps-cldn5-ocln-zo1-and-aqps-are-associated-with-brain-edema-following-fatal-heat-stroke
#7
Yu Du, Jing-Tao Xu, Hong-Nian Jin, Rui Zhao, Dong Zhao, Si-Hao Du, Ye Xue, Xiao-Li Xie, Qi Wang
Human brain samples were collected from 46 autopsy cases, including 23 fatal heat stroke cases and 23 age-matched controls. Nine candidate reference genes (PES1, POLR2A, IPO8, HMBS, SDHA, GAPDH, UBC, B2M, ACTB) were evaluated in the cerebral cortex of 10 forensic autopsy cases (5 heat stroke and 5 controls), using the geNorm module in qBase(plus) software. SDHA, POLR2A, IPO8 and HMBS were identified as the most stable reference genes. Using these validated reference genes, mRNA expressions of Matrix metalloproteinases (MMPs, MMP2 and MMP9), Claudin5 (CLDN5), Occludin (OCLN), Zona occludens protein-1 (ZO1) and Aquaporins (AQPs, AQP1 and AQP4) in the cerebral cortex were examined...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421423/the-effects-of-direct-fed-microbial-supplementation-as-an-alternative-to-antibiotics-on-growth-performance-intestinal-immune-status-and-epithelial-barrier-gene-expression-in-broiler-chickens
#8
U Gadde, S T Oh, Y S Lee, E Davis, N Zimmerman, T Rehberger, Hyun S Lillehoj
The objective of this study was to investigate the effects of Bacillus subtilis-based probiotic supplementation in broiler chicken diets on growth performance, feed efficiency, intestinal cytokine, and tight junction (TJ) protein mRNA expression. Zero-day-old broiler chicks (n = 140) were randomly assigned to one of five dietary treatments: basal diet (CON); basal diet supplemented with either antibiotic bacitracin methylene disalicylate (BMD); or probiotics, namely, B. subtilis strain 1781 (PB1), a combination of B...
April 18, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28396819/rab11-fip1-phosphorylation-by-mark2-regulates-polarity-in-mdck-cells
#9
Rebecca McRae, Lynne A Lapierre, Elizabeth H Manning, James R Goldenring
MARK2/Par1b/EMK1, a serine/threonine kinase, is required for correct apical/basolateral membrane polarization in epithelial cells. However, the specific substrates mediating MARK2 action are less well understood. We have now found that MARK2 phosphorylates Rab11-FIP1B/C at serine 234 in a consensus site similar to that previously identified in Rab11-FIP2. In MDCK cells undergoing repolarization after a calcium switch, antibodies specific for pS234-Rab11-FIP1 or pS227-Rab11-FIP2 demonstrate that the spatial and temporal activation of Rab11-FIP1 phosphorylation is distinct from that for Rab11-FIP2...
2017: Cellular Logistics
https://www.readbyqxmd.com/read/28387750/bmp6-regulates-proliferation-and-apoptosis-of-human-sertoli-cells-via-smad2-3-and-cyclin-d1-pathway-and-dach1-and-tfap2a-activation
#10
Hong Wang, Qingqing Yuan, Min Sun, Minghui Niu, Liping Wen, Hongyong Fu, Fan Zhou, Zheng Chen, Chencheng Yao, Jingmei Hou, Ruinan Shen, Qisheng Lin, Wenjie Liu, Ruobing Jia, Zheng Li, Zuping He
Sertoli cells are essential for regulating normal spermatogenesis. However, the mechanisms underlying human Sertoli cell development remain largely elusive. Here we examined the function and signaling pathways of BMP6 in regulating human Sertoli cells. RT-PCR, immunocytochemistry and Western blots revealed that BMP6 and its multiple receptors were expressed in human Sertoli cells. CCK-8 and EDU assays showed that BMP6 promoted the proliferation of Sertoli cells. Conversely, BMP6 siRNAs inhibited the division of these cells...
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28376304/origins-of-pdz-binding-specificity-a-computational-and-experimental-study-using-nherf1-and-the-parathyroid-hormone-receptor
#11
Tatyana Mamonova, Qiangmin Zhang, Mintu Chandra, Brett M Collins, Edward Sarfo, Zimei Bu, Kunhong Xiao, Alessandro Bisello, Peter A Friedman
Na(+)/H(+) exchanger regulatory factor-1 (NHERF1) is a scaffolding protein containing two PSD95/discs large protein/ZO1 (PDZ) domains that modifies the signaling, trafficking, and function of the parathyroid hormone receptor (PTHR), a family B G-protein-coupled receptor. PTHR and NHERF1 bind through a PDZ-ligand-recognition mechanism. We show that PTH elicits phosphorylation of Thr591 in the canonical -ETVM binding motif of PTHR. Conservative substitution of Thr591 with Cys does not affect PTH(1-34)-induced cAMP production or binding of PTHR to NHERF1...
May 23, 2017: Biochemistry
https://www.readbyqxmd.com/read/28251704/ochratoxin-a-cytotoxicity-on-madin-darby-canine-kidney-cells-in-the-presence-of-alpha-tocopherol-effects-on-cell-viability-and-tight-junctions
#12
E Fusi, C Giromini, R Rebucci, L Pinotti, V Caprarulo, F Cheli, F Vitari, C Domeneghini, A Baldi
Ochratoxin A (OTA) is a potent nephrotoxic fungi metabolite that affects animal and human health. At the cellular level, OTA is able to alter functions and viability by several mechanisms of action. Several strategies to counteract its toxicity have been studied. We investigated the role of α-tocopherol in counteracting OTA oxidative damage in Madin-Darby canine kidney (MDCK) cells by pre-incubating the cells for 3 hr with the antioxidant (1 nm, 10 μm) and then adding OTA (0-1.2 μg/ml) for the following 24 hr...
March 1, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28169360/loss-of-dlg5-promotes-breast-cancer-malignancy-by-inhibiting-the-hippo-signaling-pathway
#13
Jie Liu, Juan Li, Pingping Li, Yaochun Wang, Zheyong Liang, Yina Jiang, Jing Li, Chen Feng, Ruiqi Wang, He Chen, Can Zhou, Jianmin Zhang, Jin Yang, Peijun Liu
Discs Large Homolog 5 (DLG5) plays an important role in the maintenance of epithelial cell polarity. Recent research showed that DLG5 is decreased in Yes-associated protein (YAP)-overexpressing cells. However, the exact relationship between DLG5 and YAP is not clear. In this study, we showed that loss of DLG5 promoted breast cancer cell proliferation by inhibiting the Hippo signaling pathway and increasing nuclear YAP expression. Furthermore, depletion of DLG5 induced epithelial-mesenchymal transition (EMT) and disrupted epithelial cell polarity, which was associated with altered expression of Scribble, ZO1, E-cadherin and N-cadherin and their mislocalization...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28061478/mitotic-control-of-human-papillomavirus-genome-containing-cells-is-regulated-by-the-function-of-the-pdz-binding-motif-of-the-e6-oncoprotein
#14
Elizabeth K Marsh, Craig P Delury, Nicholas J Davies, Christopher J Weston, Mohammed A L Miah, Lawrence Banks, Joanna L Parish, Martin R Higgs, Sally Roberts
The function of a conserved PDS95/DLG1/ZO1 (PDZ) binding motif (E6 PBM) at the C-termini of E6 oncoproteins of high-risk human papillomavirus (HPV) types contributes to the development of HPV-associated malignancies. Here, using a primary human keratinocyte-based model of the high-risk HPV18 life cycle, we identify a novel link between the E6 PBM and mitotic stability. In cultures containing a mutant genome in which the E6 PBM was deleted there was an increase in the frequency of abnormal mitoses, including multinucleation, compared to cells harboring the wild type HPV18 genome...
March 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28054340/fingolimod-confers-neuroprotection-through-activation-of-rac1-after-experimental-germinal-matrix-hemorrhage-in-rat-pups
#15
William B Rolland, Paul R Krafft, Tim Lekic, Damon Klebe, Julia LeGrand, Abby Jones Weldon, Liang Xu, John H Zhang
Fingolimod, a sphingosine-1-phosphate receptor (S1PR) agonist, is clinically available to treat multiple sclerosis and is showing promise in treating stroke. We investigated if fingolimod provides long-term protection from experimental neonatal germinal matrix hemorrhage (GMH), aiming to support a potential mechanism of acute fingolimod-induced protection. GMH was induced in P7 rats by infusion of collagenase (0.3 U) into the right ganglionic eminence. Animals killed at 4 weeks post-GMH received low- or high-dose fingolimod (0...
March 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/27943290/isolation-characterization-and-establishment-of-spontaneously-immortalized-cell-line-hrpe-2s-with-stem-cell-properties
#16
Hoda Shams Najafabadi, Zahra-Soheila Soheili, Shahram Samiei, Hamid Ahmadieh, Ehsan Ranaei Pirmardan, Maryam Masoumi
The retinal pigment epithelium is a monolayer of highly specialized pigmented cells located between the neural retina and the Bruch's membrane of the choroid. RPE cells play a crucial role in the maintenance and function of the underlying photoreceptors. This study introduces a spontaneously arising human retinal pigment epithelial cell line, HRPE-2S, which was isolated from primary RPE cell culture of 2 days old male donor. We characterized morphology and functional properties of the new cell line. The immortalized cell line was maintained in culture for more than 70 passages and 240 divisions...
October 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27741287/establishment-of-epithelial-attachment-on-titanium-surface-coated-with-platelet-activating-peptide
#17
Shiho Sugawara, Masahiko Maeno, Cliff Lee, Shigemi Nagai, David M Kim, John Da Silva, Masazumi Nagai, Hisatomo Kondo
The aim of this study was to produce epithelial attachment on a typical implant abutment surface of smooth titanium. A challenging complication that hinders the success of dental implants is peri-implantitis. A common cause of peri-implantitis may results from the lack of epithelial sealing at the peri-implant collar. Histologically, epithelial sealing is recognized as the attachment of the basement membrane (BM). BM-attachment is promoted by activated platelet aggregates at surgical wound sites. On the other hand, platelets did not aggregate on smooth titanium, the surface typical of the implant abutment...
2016: PloS One
https://www.readbyqxmd.com/read/27722368/brain-on-a-chip-model-enables-analysis-of-human-neuronal-differentiation-and-chemotaxis
#18
Onur Kilic, David Pamies, Emily Lavell, Paula Schiapparelli, Yun Feng, Thomas Hartung, Anna Bal-Price, Helena T Hogberg, Alfredo Quinones-Hinojosa, Hugo Guerrero-Cazares, Andre Levchenko
Migration of neural progenitors in the complex tissue environment of the central nervous system is not well understood. Progress in this area has the potential to drive breakthroughs in neuroregenerative therapies, brain cancer treatments, and neurodevelopmental studies. To a large extent, advances have been limited due to a lack of controlled environments recapitulating characteristics of the central nervous system milieu. Reductionist cell culture models are frequently too simplistic, and physiologically more relevant approaches such as ex vivo brain slices or in situ experiments provide little control and make information extraction difficult...
October 18, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27645110/identification-of-novel-transplantable-gpcr-recycling-motif-for-drug-discovery
#19
COMPARATIVE STUDY
Mohammed M Nooh, Salvatore Mancarella, Suleiman W Bahouth
β1-Adrenergic receptor (β1-AR) agonists and antagonists are widely used in the treatment of major cardiovascular diseases such as heart failure and hypertension. The β1-AR like other G protein-coupled receptors (GPCRs) are endocytosed in response to intense agonist activation. Recycling of the agonist-internalized β1-AR is dependent on its carboxy-terminal type-1 PSD-95/DLG/ZO1 (PDZ) and on phospho-serine(312) in the third intracellular loop of the β1-AR. Progressive elongation of the β1-AR at its C-tail inactivated the PDZ-biding domain and inhibited the recycling of the β1-AR...
November 15, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/27602775/epithelialization-of-mouse-ovarian-tumor-cells-originating-in-the-fallopian-tube-stroma
#20
Yuanyuan Hua, Pui-Wah Choi, Alexander J Trachtenberg, Allen C Ng, Winston P Kuo, Shu-Kay Ng, Daniela M Dinulescu, Martin M Matzuk, Ross S Berkowitz, Shu-Wing Ng
Epithelial ovarian carcinoma accounts for 90% of all ovarian cancer and is the most deadly gynecologic malignancy. Recent studies have suggested that fallopian tube fimbriae can be the origin of cells for high-grade serous subtype of epithelial ovarian carcinoma (HGSOC). A mouse HGSOC model with conditional Dicer-Pten double knockout (Dicer-Pten DKO) developed primary tumors, intriguingly, from the fallopian tube stroma. We examined the growth and epithelial phenotypes of the Dicer-Pten DKO mouse tumor cells contributable by each gene knockout...
October 4, 2016: Oncotarget
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