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IVF air quality

Hiroki Kurosawa, Hiroki Utsunomiya, Naomi Shiga, Aiko Takahashi, Motomasa Ihara, Masumi Ishibashi, Mitsuo Nishimoto, Zen Watanabe, Hiroyuki Abe, Jin Kumagai, Yukihiro Terada, Hideki Igarashi, Toshifumi Takahashi, Atsushi Fukui, Ryota Suganuma, Masahito Tachibana, Nobuo Yaegashi
STUDY QUESTION: Does a new system-the chip-sensing embryo respiration monitoring system (CERMs)-enable evaluation of embryo viability for potential application in a clinical IVF setting? SUMMARY ANSWER: The system enabled the oxygen consumption rate of spheroids, bovine embryos and frozen-thawed human embryos to be measured, and this rate corresponded to the developmental potential of embryos. WHAT IS ALREADY KNOWN: To date, no reliable and clinically suitable objective evaluation methods for embryos are available, which circumvent the differences in inter-observer subjective view...
October 2016: Human Reproduction
Miguel A Checa Vizcaíno, Mireia González-Comadran, Benedicte Jacquemin
Air pollution is a current research priority because of its adverse effects on human health, including on fertility. However, the mechanisms through which air pollution impairs fertility remain unclear. In this article, we perform a systematic review to evaluate currently available evidence on the impact of air pollution on fertility in humans. Several studies have assessed the impact of air pollutants on the general population, and have found reduced fertility rates and increased risk of miscarriage. In subfertile patients, women exposed to higher concentrations of air pollutants while undergoing IVF showed lower live birth rates and higher rates of miscarriage...
September 15, 2016: Fertility and Sterility
G Zullo, C De Canditiis, M E Pero, G Albero, A Salzano, G Neglia, G Campanile, B Gasparrini
The aim of this work was to assess the effect of supplementation of bovine culture medium with the natural antioxidant crocetin on in vitro blastocyst development and quality. This was evaluated as cryotolerance, apoptosis index, and total cells number and allocation. Abattoir-derived oocytes were matured and fertilized in vitro according to standard procedure. Twenty hours after IVF, presumptive zygotes were cultured in synthetic oviduct fluid medium, supplemented with 0, 1, 2.5, and 5 μM crocetin (experiment 1) at 39 °C under humidified air with 5% CO2, 7% O2, and 88% N2...
November 2016: Theriogenology
Marjan Omidi, Iman Halvaei, Esmat Mangoli, Mohammad Ali Khalili, Mohammad Hossein Razi
OBJECTIVE: Embryo loading (EL) is a major step in embryo transfer (ET) and affect on the success of in vitro fertilization (IVF). This study aimed to compare the effect of two different EL techniques on the rates of pregnancy and delivery in IVF/ET cycles. METHODS: 207 fresh ET and 194 Frozen-thawed ET (FET) cycles were included in this retrospective study. Two groups (A and B) were defined based on the EL technique used. In group A, the entire catheter was flushed with Ham's F-10 medium...
December 2015: Clinical and Experimental Reproductive Medicine
Nuno Costa-Borges, Marta Bellés, Marcos Meseguer, Daniela Galliano, Agustin Ballesteros, Gloria Calderón
OBJECTIVE: To evaluate the efficiency of using a continuous (one-step) protocol with a single medium for the culture of human embryos in a time-lapse incubator (TLI). DESIGN: Prospective cohort study on sibling donor oocytes. SETTING: University-affiliated in vitro fertilization (IVF) center. PATIENT(S): Embryos from 59 patients. INTERVENTION(S): Culture in a TLI in a single medium with or without renewal of the medium on day-3...
March 2016: Fertility and Sterility
Sandro C Esteves, Fabiola C Bento
Two articles recently published in Reproductive BioMedicine Online described how fertility centres in the USA and Brazil implemented air quality control to newly designed facilities. In both case scenarios, a highly efficient air filtration was achieved by installing a centred system supplying filtered air to the IVF laboratory and other critical areas, combining air particulate and volatile organic compound (VOC) filtration. Evaluating retrospective data of over 3000 cycles from both centres, live birth rates were increased by improvements in air quality and laboratory environment...
January 2016: Reproductive Biomedicine Online
Sandro C Esteves, Fabiola C Bento
No abstract text is available yet for this article.
July 2016: Asian Journal of Andrology
G Zullo, G Albero, G Neglia, C De Canditiis, G Bifulco, G Campanile, B Gasparrini
The aim of this study was to evaluate whether supplementation of bovine culture medium with the natural antioxidant L-ergothioneine (LE), improves in vitro blastocyst development and quality, assessed as resistance to cryopreservation, total cells number, cellular differentiation, and apoptosis index. Abattoir-derived oocytes were matured and fertilized in vitro according to standard procedure. Twenty hours after IVF, presumptive zygotes were cultured in synthetic oviduct fluid with 0, 0.05 mM, 0.1 mM, 0.5 mM, and 1 mM of LE (experiment 1) at 39 °C under humidified air with 5% CO2, 7% O2, and 88% N2...
March 1, 2016: Theriogenology
Dean E Morbeck
Quality of air in the clinical embryology laboratory is considered critical for high in vitro fertilization (IVF) success rates, yet evidence for best practices is lacking. Predominantly anecdotal reports on relationships between air quality and IVF success rates have resulted in minimal authentic clinical laboratory guidelines or in recommendations that are based on industrial cleanroom particulate standards with little attention to chemical air filtration. As a result, a nascent industry of costly, specialized air handling equipment for IVF laboratories has emerged to provide air quality solutions that have not been clearly assessed or verified...
July 2015: Journal of Assisted Reproduction and Genetics
Ryan J Heitmann, Micah J Hill, Aidita N James, Tim Schimmel, James H Segars, John M Csokmay, Jacques Cohen, Mark D Payson
Infertility is a common disease, which causes many couples to seek treatment with assisted reproduction techniques. Many factors contribute to successful assisted reproduction technique outcomes. One important factor is laboratory environment and air quality. Our facility had the unique opportunity to compare consecutively used, but separate assisted reproduction technique laboratories, as a result of a required move. Environmental conditions were improved by strategic engineering designs. All other aspects of the IVF laboratory, including equipment, physicians, embryologists, nursing staff and protocols, were kept constant between facilities...
September 2015: Reproductive Biomedicine Online
Zhao-Feng Peng, Sen-Lin Shi, Hai-Xia Jin, Gui-Dong Yao, En-Yin Wang, Hong-Yi Yang, Wen-Yan Song, Ying-Pu Sun
The aim of the present study was to determine the impact of oxygen concentration during in vitro culture of human oocytes and embryos on fertilization, cleavage, implantation, pregnancy, multiple gestation and abortion rates. Women 20-48 years old presenting for infertility treatment and accounting for 3484 in vitro fertilization/intracytoplasmic sperm injection cycles were included in the study. Oocytes/embryos were randomly allocated to be incubated under three different oxygen tension environments: (1) 20% O2 in air; (2) initially 20% O2 in air, followed on day 2 (2-4 cells stage) by 5% CO2, 5% O2 and 90% N2; and (3) 5% CO2, 5% O2 and 90% N2 throughout...
2015: International Journal of Clinical and Experimental Medicine
Víctor Frutos, Mireia González-Comadrán, Ivan Solà, Benedicte Jacquemin, Ramón Carreras, Miguel A Checa Vizcaíno
Air pollution has gained considerable interest because of the multiple adverse effects reported on human health, although its impact on fertility remains unclear. A systematic search was performed to evaluate the impact of air pollutants on fertility. Controlled trials and observational studies assessing animal model and epidemiological model were included. Occupational exposure and semen quality studies were not considered. Outcomes of interest included live birth, miscarriage, clinical pregnancy, implantation, and embryo quality...
January 2015: Gynecological Endocrinology
Ahmed S Abdoon, Christoph Gabler, Christoph Holder, Omaima M Kandil, Ralf Einspanier
Hot season is a major constraint to production and reproduction in buffaloes. The present work aimed to investigate the effect of season on ovarian function, developmental competence, and the relative abundance of gene expression in buffalo oocytes. Three experiments were conducted. In experiment 1, pairs of buffalo ovaries were collected during cold season (CS, autumn and winter) and hot season (HS, spring and summer), and the number of antral follicles was recorded. Cumulus oocyte complexes (COCs) were aspirated and evaluated according to their morphology into four Grades...
November 2014: Theriogenology
Mark J Nieuwenhuijsen, Xavier Basagaña, Payam Dadvand, David Martinez, Marta Cirach, Rob Beelen, Bénédicte Jacquemin
BACKGROUND: Some reports have suggested effects of air pollution on semen quality and success rates of in vitro fertilization (IVF) in humans and lower fertility rates in mice. However, no studies have evaluated the impact of air pollution on human fertility rates. AIMS: We assessed the association between traffic related air pollution and fertility rates in humans in Barcelona, Spain (2011-2012). We hypothesized that higher air pollution levels would be associated with lower fertility rates...
September 2014: Environment International
Maria J de los Santos, Pilar Gámiz, Carmela Albert, Arancha Galán, Thamara Viloria, Sonia Pérez, Josep Ll Romero, José Remohï
OBJECTIVE: To investigate the effect of low O2 tension during in vitro culture in terms of ongoing pregnancy rates in ovum donation cycles. DESIGN: Randomized trial. SETTING: Private university-affiliated IVF center, university-based hospital. PATIENT(S): A total of 1,125 cycles of ovum donation. INTERVENTION(S): Embryo culture in an atmosphere of 5.5% CO2, 6% O2, and 88.5% N2 versus a dual-gas system of 5...
August 2013: Fertility and Sterility
B A Rodrigues, C A Rodrigues, M B Salviano, B R Willhelm, F J F Collares, J L Rodrigues
The influence of two oxygen tensions (5 and 20%) on fertilization, cleavage, development, and morphological quality of canine embryos produced in vitro was investigated. To assess embryo production, canine oocytes (N = 608) were matured in vitro at 20% oxygen tension in TCM 199 supplemented with glucose (11 mM) and 0.1% polyvinyl alcohol. Oocytes and sperm were coincubated at 37 °C for 24 hours in serum-free medium. Subsequently, presumptive zygotes were cultured in vitro in synthetic oviductal fluid in either 5% CO2 in air (20% oxygen; N = 298) or 5% O2, 5% CO2 and 90% N2 (5% oxygen; N = 310) for 7 days...
May 2013: Theriogenology
Rabea Youcef Khoudja, Yanwen Xu, Tao Li, Canquan Zhou
BACKGROUND: It has been proved that air quality is crucial for the success of IVF because of the presence of volatile organic compounds (VOCs), microbes, and perfumes, all of which can be harmful to embryo development in vitro. Therefore IVF laboratories are equipped with high efficiency particulate air (HEPA), and activated carbon filters plus positive pressure for air particulate control, with or without CODA system. Here we introduce a new technology using specially treated Honeycomb matrix media aligned in the Landson ™ series system for our laboratory air purification and its impact on IVF outcome...
January 2013: Journal of Assisted Reproduction and Genetics
Sandro C Esteves, Fabiola C Bento
This article describes how Androfert complied with the Brazilian Cells and Germinative Tissue Directive with regard to air quality standards and presents retrospective data of intracytoplasmic sperm injection (ICSI) outcomes performed in controlled environments. An IVF facility, composed of reproductive laboratories, operating room and embryo-transfer room, was constructed according to cleanroom standards for air particles and volatile organic compounds. A total of 2060 couples requesting IVF were treated in the cleanroom facilities, and outcome measures compared with a cohort of 255 couples treated at a conventional facility from the same practice before implementation of cleanrooms...
January 2013: Reproductive Biomedicine Online
E Dovolou, M Clemente, G S Amiridis, I E Messinis, A Kallitsaris, A Gutierrez-Adan, D Rizos
Reactive oxygen species (ROS) are between the major contributors for the reduced rate of in vitro bovine embryo production. It is believed that they can cause abnormal meiosis of oocytes, developmental arrest or cell death of embryos. Reports on the effectiveness of various antioxidants on embryo yield are rather conflicting mainly due to the nature and the concentration of the substances used. Here we report the effects of guaiazulene--an exogenous antioxidant, without known properties that could interfere with the biological process of IVF--on embryo development and on the quality of the produced blastocysts...
October 2011: Reproduction in Domestic Animals, Zuchthygiene
Bradley J Van Voorhis, Mika Thomas, Eric S Surrey, Amy Sparks
OBJECTIVE: To identify common clinical and laboratory practices among consistently high-performing IVF programs. DESIGN: Questionnaire study of selected IVF programs. SETTING: Academic and private practice IVF programs. PATIENT(S): Ten of 12 programs identified as having consistently high singleton delivery rates per cycle. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Common clinical practices...
September 2010: Fertility and Sterility
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