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https://www.readbyqxmd.com/read/29029218/dose-dependent-effects-on-replication-and-persistence-of-salmonella-enterica-serovar-typhimurium-in-house-flies-diptera-muscidae
#1
Rabecca Chifanzwa, Dana Nayduch
Adult house flies (Musca domestica L.) ingest variable numbers of bacteria when they encounter microbe-rich substrates. Bacterial abundance may affect replication within the fly gut, which subsequently impacts vector potential. This study investigated the dose-dependent replication of GFP-expressing Salmonella enterica serovar Typhimurium (ex Kauffmann and Edwards1952) Le Minor and Popoff 1987, (Enterobacteriales: Enterobacteriaceae) (GFP S. Typhimurium) within the fly alimentary canal. Adult house flies were fed two doses (colony forming units, CFU) of GFP S...
October 4, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29029093/electronegative-low-density-lipoprotein-l5-induces-adipose-tissue-inflammation-associated-with-metabolic-syndrome
#2
Liang-Yin Ke, Hua-Chen Chan, Hsiu-Chuan Chan, Franklin Chikodi Udo Kalu, Hsiang-Chun Lee, I-Ling Lin, Shih-Jie Jhuo, Wen-Ter Lai, Chen-Rong Tsao, Tatsuya Sawamura, Richard A Dixon, Chu-Huang Chen, Chih-Sheng Chu, Shyi-Jang Shin
Context: Electronegative low-density lipoprotein (LDL) L5 is a naturally occurring, atherogenic entity found at elevated levels in the plasma of patients with metabolic syndrome (MetS) in the absence of elevated plasma LDL levels. Objective: To investigate the role of L5 in the mechanism of adipose tissue inflammation associated with MetS. Patients/Setting: Plasma LDL isolated from MetS patients (n=29) and controls (n=29) with similar plasma LDL levels was separated into 5 subfractions, L1-L5, with increasing electronegativity...
September 28, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29027121/harnessing-the-effect-of-ph-on-lipid-production-in-batch-cultures-of-yarrowia-lipolytica-sky7
#3
Mathiazhakan Kuttiraja, Ayed Dhouha, Rajeshwar Dayal Tyagi
The objective of this research was to investigate the kinetics of lipid production by Yarrowia lipolytica SKY7 in the crude glycerol-supplemented media with and without the control of pH. Lipid and citric acid production were improved with the pH control condition. There was no significant difference observed in the biomass concentration with or without the pH control. In the pH-controlled experiments, the biomass and lipid concentration reached 18 and 7.78 g/L, (45.5% w/w), respectively, with lipid yield (Yp/s) of 0...
October 12, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29026717/addition-of-interleukin-6-to-mouse-embryo-culture-increases-blastocyst-cell-number-and-influences-the-inner-cell-mass-to-trophectoderm-ratio
#4
Rebecca L Kelley, David K Gardner
OBJECTIVE: In vitro culture of preimplantation embryos is improved by grouping embryos together in a drop of media. Individually cultured embryos are deprived of paracrine factors; with this in mind, we investigated whether the addition of a single embryo-secreted factor, interleukin-6 (IL-6), could improve the development of individually cultured embryos. METHODS: Mouse embryos were cultured individually in 2 µL of G1/G2 media in 5% oxygen and supplemented with a range of doses of recombinant mouse or human IL-6...
September 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29026707/nadph-oxidase-mediates-%C3%AE-amyloid-peptide-induced-neuronal-death-in-mouse-cortical-cultures
#5
Kee-Oh Chay, Kyoung Young Nam Koong, Shinae Hwang, Jong-Keun Kim, Choon Sang Bae
β-Amyloid peptide (Aβ) is the main component of senile plaques in patients with Alzheimer's disease, and is known to be a main pathogenic factor of the disease. Recent evidence indicates that activation of NADPH oxidase (NOX) in microglia or astrocytes may be a source of Aβ-induced reactive oxygen species (ROS). We investigated the role of neuronal NOX in Aβ-induced neuronal death in mouse mixed cortical cultures. Cell death was assessed by measuring lactate dehydrogenase efflux to bathing media 24 or 48 hr after exposure to Aβ25-35, a fragment of Aβ with an equivalent neurotoxic effect...
September 2017: Chonnam Medical Journal
https://www.readbyqxmd.com/read/29026640/directing-the-osteoblastic-and-chondrocytic-differentiations-of-mesenchymal-stem-cells-matrix-vs-induction-media
#6
Jing He, Jianglong Guo, Bo Jiang, Ruijuan Yao, Yao Wu, Fang Wu
While both induction culture media and matrix have been reported to regulate the stem cell fate, little is known about which factor plays a more decisive role in directing the MSC differentiation lineage as well as the underlying mechanisms. To this aim, we seeded MSCs on HA-collagen and HA-synthetic hydrogel matrixes, which had demonstrated highly different potentials toward osteoblastic and chondrocytic differentiation lineages, respectively, and cultured them with osteogenic, chondrogenic and normal culture media, respectively...
October 2017: Regenerative Biomaterials
https://www.readbyqxmd.com/read/29026626/acute-diarrhoea-due-to-a-shiga-toxin-2e-producing-escherichia-coli-o8%C3%A2-%C3%A2-h19
#7
Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel
Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx2e-positive STEC of serotype O8 : H19...
June 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29026146/multiple-approaches-detect-the-presence-of-fungi-in-human-breastmilk-samples-from-healthy-mothers
#8
Alba Boix-Amorós, Cecilia Martinez-Costa, Amparo Querol, Maria Carmen Collado, Alex Mira
Human breastmilk contains a variety of bacteria that are transmitted to the infant and have been suggested to contribute to gut microbiota development and immune maturation. However, the characterization of fungal organisms in milk from healthy mothers is currently unknown although their presence has been reported in the infant gut and also in milk from other mammals. Breastmilk samples from healthy lactating mothers (n = 65) within 1 month after birth were analyzed. Fungal presence was assessed by different techniques, including microscopy, growth and identification of cultured isolates, fungal load estimation by qPCR, and fungal composition using 28S rRNA gene high-throughput sequencing...
October 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29025701/distribution-of-h3k27me3-h3k9me3-and-h3k4me3-along-autophagy-related-genes-highly-expressed-in-starved-zebrafish-myotubes
#9
Peggy R Biga, Mary N Latimer, Jacob Michael Froehlich, Jean-Charles Gabillard, Iban Seiliez
The zebrafish (Danio rerio) remains the teleost fish of choice for biological investigations due to the vast array of molecular tools and resources available. To better understand the epigenetic regulation of autophagy, we utilized a primary myotube culture system generated from isolated myogenic precursor cells (MPCs) from zebrafish grown under starvation conditions using a media devoid of serum and amino acids. Here, we report starvation-induced regulation of several autophagy-related genes (atg) expression and profile the distribution of H3K27me3, H3K9me3, and H3K4me3 marks along lc3b, atg4b and p62/sqstm1 loci...
October 12, 2017: Biology Open
https://www.readbyqxmd.com/read/29024547/equine-endometrial-explants-undergo-significant-degenerative-changes-in-culture
#10
Rose Schwinghamer, Alessandro Massolo, Cameron Knight, Claudia Klein
The current study evaluated equine endometrial explants following 12, 24, and 48 hours in culture. Measurement of an indicator of cell death in explant supernatant, light microscopy, and gene expression of biomarkers of endometrial function and cellular stress, were used to compare the effect of six different media on explant viability and morphology. Viability of explants was assessed indirectly through measuring LDH activity in the culture supernatant. Regardless of culture medium composition, a significant increase in LDH activity was observed within 12 hours of culture, indicating occurrence of cell damage...
October 12, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29023839/effectiveness-of-a-brief-home-parenting-intervention-for-reducing-early-sexual-risks-among-latino-adolescents-salud-y-%C3%A3-xito
#11
Lydia O'Donnell, Shai Fuxman
BACKGROUND: Teen pregnancy rates and related risks remain elevated among Latino teens. We tested the impact on youth sexual behaviors of a brief, culturally targeted, bilingual media intervention designed for parents of young adolescents. METHODS: Salud y éxito (Health & Success) uses dramatic audio stories to model positive parenting practices. After completing classroom surveys, 27 urban schools in the Northeast and Southwest serving low-income Latino communities were randomized so that all families of seventh grade students were sent either: (1) booklets on healthy eating and exercise; (2) Salud-50, where families either received booklets or the intervention, or (3) Salud-100, where all families received the intervention...
November 2017: Journal of School Health
https://www.readbyqxmd.com/read/29023204/bury-your-gays-and-social-media-fan-response-television-lgbtq-representation-and-communitarian-ethics
#12
Erin B Waggoner
While visibility has increased, there are still harmful television tropes used that can have potentially harmful effects on LGBTQ persons seeking identification through television characters. This essay explores women-loving women (wlw) representation in television and how fans respond via social media with regards to their identity and representation. Specifically, by examining the fan culture through a communitarian ethics lens (with an emphasis on duty and mutuality), fans are explored pre and post a lesbian character's death and how their responses created a social movement regarding the current television environment's treatment of wlw characters...
October 12, 2017: Journal of Homosexuality
https://www.readbyqxmd.com/read/29023011/effect-of-agno3-and-bap-on-root-as-a-novel-explant-in-date-palm-phoenix-dactylifera-cv-medjool-somatic-embryogenesis
#13
Marjan Roshanfekrrad, Reza Zarghami, Hassan Hassani, Hedayat Zakizadeh, Ali Salari
BACKGROUND AND OBJECTIVE: Somatic embryogenesis techniques are used for cloning a wide range of varieties of date palms around the world. The aim of the present study was to develop an efficient method with the lowest cost and the greatest potential to obtain in vitro plantlets of date palm cv. Medjool. Also, produce embryogenic callus and somatic embryos without using 2,4-dichlorophenoxyacetic acid (2,4-D). METHODOLOGY: In this study, produced plantlets through somatic embryogenesis were used in vitro roots as explant cultured on Murashige and Skoog (MS) media containing three level of Silver Nitrate (AgNO3) (0, 3 and 6 mg L-1) plus two level of 6-benzylaminopurine (BAP) (0 and 2 mg L-1) plus 0...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29023007/optimization-of-culture-medium-for-the-growth-of-candida-sp-and-blastobotrys-sp-as-starter-culture-in-fermentation-of-cocoa-beans-theobroma-cacao-using-response-surface-methodology-rsm
#14
N H Mahazar, Z Zakuan, H Norhayati, A S MeorHussin, Y Rukayadi
BACKGROUND AND OBJECTIVE: Inoculation of starter culture in cocoa bean fermentation produces consistent, predictable and high quality of fermented cocoa beans. It is important to produce healthy inoculum in cocoa bean fermentation for better fermented products. Inoculum could minimize the length of the lag phase in fermentation. The purpose of this study was to optimize the component of culture medium for the maximum cultivation of Candida sp. and Blastobotrys sp. MATERIALS AND METHODS: Molasses and yeast extract were chosen as medium composition and Response Surface Methodology (RSM) was then employed to optimize the molasses and yeast extract...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29022742/culture-based-quantification-with-molecular-characterization-of-non-o157-and-o157-enterohemorrhagic-escherichia-coli-isolates-from-rectoanal-mucosal-swabs-of-feedlot-cattle
#15
Zachary R Stromberg, Gentry L Lewis, Liesel G Schneider, Galen E Erickson, Isha R Patel, David R Smith, Rodney A Moxley
Enterohemorrhagic Escherichia coli (EHEC) strains are foodborne pathogens carried in the intestinal tracts of ruminants and shed in the feces. High concentrations (≥10(4) colony-forming units [CFU]/g) of EHEC in cattle feces are associated with contamination of hides, and subsequently, carcasses and beef. Several studies using agar media have quantified O157 but few have quantified non-O157 EHEC in samples from cattle. Thus, the objective of this study was to determine the concentration of O157 and non-O157 EHEC in cattle, and to characterize the associated EHEC isolates for their virulence potential...
October 12, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29022242/exploring-the-role-of-youtube-in-disseminating-psychoeducation
#16
Nikki Hei Tong Lam, John Ta-Hsiang Tsiang, Benjamin K P Woo
OBJECTIVE: Social media can bridge the gap between health care and ethnic minorities over cultural barriers. This study explores the role of YouTube in delivering schizophrenia education to individuals in the USA who are also fluent in Chinese. METHODS: Three psychoeducational YouTube videos related to schizophrenia were uploaded. Data were collected for a 12-month period, and results were analyzed using descriptive statistics. RESULTS: The videos recorded 4935 views with a total viewing time of 35,614 min...
October 11, 2017: Academic Psychiatry
https://www.readbyqxmd.com/read/29022110/monitoring-bone-tissue-engineered-bte-constructs-based-on-the-shifting-metabolism-of-differentiating-stem-cells
#17
Aaron D Simmons, Vassilios I Sikavitsas
Ever-increasing demand for bone grafts necessitates the realization of clinical implementation of bone tissue engineered constructs. The predominant hurdle to implementation remains to be securing FDA approval, based on the lack of viable methods for the rigorous monitoring of said constructs. The study presented herein details a method for such monitoring based on the shifting metabolism of mesenchymal stem cells (MSCs) as they differentiate into osteoblasts. To that end, rat MSCs seeded on 85% porous spunbonded poly(L-lactic acid) scaffolds were cultured in flow perfusion bioreactors with baseline or osteoinductive media, and levels of key physio-metabolic markers (oxygen, glucose, osteoprotegerin, and osteocalcin) were monitored throughout culture...
October 11, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29021946/predictors-of-pneumococcal-carriage-and-the-effect-of-the-13-valent-pneumococcal-conjugate-vaccination-in-the-western-australian-aboriginal-population
#18
Deirdre A Collins, Anke Hoskins, Thomas Snelling, Kalpani Senasinghe, Jacinta Bowman, Natalie A Stemberger, Amanda J Leach, Deborah Lehmann
BACKGROUND: The 7-valent pneumococcal conjugate vaccine (PCV7) was introduced to prevent invasive pneumococcal disease (IPD) in Western Australian (WA) Aboriginal people in 2001. PCV13 replaced PCV7 in July 2011, covering six additional pneumococcal serotypes; however, IPD rates remained high in Aboriginal people in WA. Upper respiratory tract pneumococcal carriage can precede IPD, and PCVs alter serotype distribution. METHODS: To assess the impact of PCV13 introduction, identify emerging serotypes, and assess risk factors for carriage, nasopharyngeal swabs and information on demographic characteristics, health, medication and living conditions from Aboriginal children and adults across WA from August 2008 to November 2014 were collected...
2017: Pneumonia
https://www.readbyqxmd.com/read/29020385/a-process-evaluation-of-the-aware-and-supportive-communities-gambling-harm-minimisation-programmes-in-new-zealand
#19
Komathi Kolandai-Matchett, Maria Bellringer, Jason Landon, Max Abbott
Background: The Gambling Act 2003 mandated a public health strategy for preventing and minimising gambling harm in New Zealand . Aware Communities and Supportive Communities are two public health programmes subsequently implemented nationwide. These programmes differed from common health promotion initiatives such as media or education campaigns as they were community-action based (requiring community involvement in programme planning and delivery). We carried out a process evaluation to determine their implementation effectiveness and inform improvement and future programme planning...
August 31, 2017: European Journal of Public Health
https://www.readbyqxmd.com/read/29020218/pan-serotype-reduction-in-progression-of-streptococcus-pneumoniae-to-otitis-media-after-rollout-of-pneumococcal-conjugate-vaccines
#20
Joseph A Lewnard, Noga Givon-Lavi, Daniel M Weinberger, Marc Lipsitch, Ron Dagan
Background: Reductions in otitis media (OM) burden following rollout of pneumococcal conjugate vaccines (PCVs) have exceeded predictions of vaccine impact. In settings with active surveillance, reductions in OM caused by vaccine-targeted pneumococcal serotypes have co-occurred with reductions in OM caused by other pathogens carried in the upper-respiratory tract of children. To understand these changes, we investigated the progression of vaccine-targeted and non-vaccine pneumococcal serotypes from carriage to OM before and after vaccine rollout...
July 29, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
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