keyword
MENU ▼
Read by QxMD icon Read
search

Culture Media

keyword
https://www.readbyqxmd.com/read/29471395/origin-and-composition-of-cell-free-dna-in-spent-medium-from-human-embryo-culture-during-preimplantation-development
#1
M Vera-Rodriguez, A Diez-Juan, J Jimenez-Almazan, S Martinez, R Navarro, V Peinado, A Mercader, M Meseguer, D Blesa, I Moreno, D Valbuena, C Rubio, C Simon
STUDY QUESTION: What is the origin and composition of cell-free DNA in human embryo spent culture media? SUMMARY ANSWER: Cell-free DNA from human embryo spent culture media represents a mix of maternal and embryonic DNA, and the mixture can be more complex for mosaic embryos. WHAT IS KNOWN ALREADY: In 2016, ~300 000 human embryos were chromosomally and/or genetically analyzed using preimplantation genetic testing for aneuploidies (PGT-A) or monogenic disorders (PGT-M) before transfer into the uterus...
February 20, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29471108/redox-stress-in-marfan-syndrome-dissecting-the-role-of-the-nadph-oxidase-nox4-in-aortic-aneurysm
#2
Francesc Jiménez-Altayó, Thayna Meirelles, Eva Crosas-Molist, M Alba Sorolla, Darya Gorbenko Del Blanco, Judit López-Luque, Aleksandra Mas-Stachurska, Ana-Maria Siegert, Fabio Bonorino, Laura Barberà, Carolina García, Enric Condom, Marta Sitges, Fernando Rodríguez-Pascual, Francisco Laurindo, Katrin Schröder, Joaquim Ros, Isabel Fabregat, Gustavo Egea
Marfan syndrome (MFS) is characterized by the formation of ascending aortic aneurysms resulting from altered assembly of extracellular matrix fibrillin-containing microfibrils and dysfunction of TGF-β signaling. Here we identify the molecular targets of redox stress in aortic aneurysms from MFS patients, and investigate the role of NOX4, whose expression is strongly induced by TGF-β, in aneurysm formation and progression in a murine model of MFS. Working models included aortae and cultured vascular smooth muscle cells (VSMC) from MFS patients, and a NOX4-deficient Marfan mouse model (Fbn1C1039G/+ -Nox4-/- )...
February 19, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29469223/mitoprotective-therapy-preserves-chondrocyte-viability-and-prevents-cartilage-degeneration-in-an-ex-vivo-model-of-posttraumatic-osteoarthritis
#3
Michelle L Delco, Edward D Bonnevie, Hazel S Szeto, Lawrence J Bonassar, Lisa A Fortier
No disease-modifying osteoarthritis (OA) drugs are available to prevent posttraumatic osteoarthritis (PTOA). Mitochondria (MT) mediate the pathogenesis of many degenerative diseases, and recent evidence indicates that MT dysfunction is a peracute (within minutes to hours) response of cartilage to mechanical injury. The goal of this study was to investigate cardiolipin-targeted mitoprotection as a new strategy to prevent chondrocyte death and cartilage degeneration after injury. Cartilage was harvested from bovine knee joints and subjected to a single, rapid impact injury (24...
February 22, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29468814/effects-of-substrate-stiffness-on-dental-pulp-stromal-cells-in-culture
#4
L Datko Williams, A Farley, M Cupelli, S Alapati, M S Kennedy, D Dean
Dental pulp stromal cells (DPSCs) can be differentiated down lineages known to either express bone or dentin specific protein markers. . Since the differentiation of cells can be heavily influenced by their environment, it may be possible to influence the osteogenic/odontogenic potential of DPSCs by modulating the mechanical properties of substrate on which they are grown. In this study, human DPSCs were grown with and without hydroxyapatite (HA) microparticles on a range of substrates including fibronectin-coated hydrogels and glass substrates, which represented an elastic moduli range of ∼ 3 kPa - 50 GPa, over a 21 day period...
February 22, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29467747/metabolic-engineering-for-enhanced-medium-chain-omega-hydroxy-fatty-acid-production-in-escherichia-coli
#5
Kang Xiao, Xiu-Hong Yue, Wen-Chao Chen, Xue-Rong Zhou, Lian Wang, Lin Xu, Feng-Hong Huang, Xia Wan
Medium chain hydroxy fatty acids (HFAs) at ω-1, 2, or 3 positions (ω-1/2/3) are rare in nature but are attractive due to their potential applications in industry. They can be metabolically engineered in Escherichia coli , however, the current yield is low. In this study, metabolic engineering with P450BM3 monooxygenase was applied to regulate both the chain length and sub-terminal position of HFA products in E. coli , leading to increased yield. Five acyl-acyl carrier protein thioesterases from plants and bacteria were first evaluated for regulating the chain length of fatty acids...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29467541/inhibition-of-endo-lysosomal-function-exacerbates-vascular-calcification
#6
Yujun Cai, Xue-Lin Wang, Alyssa M Flores, Tonghui Lin, Raul J Guzman
Vascular calcification is a pathologic response to mineral imbalances and is prevalent in atherosclerosis, diabetes mellitus, and chronic kidney disease. When located in the media, it is highly associated with increased cardiovascular morbidity and mortality, particularly in patients on dialysis. Vascular calcification is tightly regulated and controlled by a series of endogenous factors. In the present study, we assess the effects of lysosomal and endosomal inhibition on calcification in vascular smooth muscle cells (VSMCs) and aortic rings...
February 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29466661/heterogeneity-in-nitrogen-sources-enhances-productivity-and-nutrient-use-efficiency-in-algal-polycultures
#7
Shovon Mandal, Jonathan B Shurin, Rebecca Efroymson, Teresa Mathews
Algae hold much promise as a potential feedstock for biofuels and other products, but scaling up biomass production remains challenging. We hypothesized that multispecies assemblages, or polycultures, could improve crop yield when grown in media with mixed nitrogen sources, as found in wastewater. We grew mono- and poly- cultures of algae in four distinct growth media that differed in the form (i.e. nitrate, ammonium, urea, plus a mixture of all three), but not the concentration of nitrogen. We found that mean biomass productivity was positively correlated with algal species richness, and that this relationship was strongest in mixed nitrogen media (on average 88% greater biomass production in 5-species polycultures than in monocultures in mixed nitrogen treatment)...
February 21, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29466620/secretome-analysis-of-hypoxia-induced-3t3-l1-adipocytes-uncovers-novel-proteins-potentially-involved-in-obesity
#8
Anna Elisa Laria, Sebastiano Messineo, Biagio Arcidiacono, Mariaconcetta Varano, Eusebio Chiefari, Robert K Semple, Nuno Rocha, Diego Russo, Giovanni Cuda, Marco Gaspari, Antonio Brunetti, Daniela P Foti
In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of adipocyte-secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of murine differentiated 3T3-L1 adipocytes exposed to normoxia or hypoxia for 24 h. Proteins secreted into the culture media were precipitated by trichloroacetic acid and then digested with trypsin. The peptides were labeled with dimethyl labeling and analyzed by reversed phase nanoscale liquid chromatography coupled to a quadrupole Orbitrap mass spectrometer...
February 21, 2018: Proteomics
https://www.readbyqxmd.com/read/29466508/comparative-study-of-three-xenic-media-culture-for-cultivation-of-balantidium-coli-strains
#9
Alynne da Silva Barbosa, Matheus Lessa Cardozo, Laís Verdan Dib, Ana Beatriz Monteiro Fonseca, Claudia Maria Antunes Uchôa, Otilio Machado Pereira Bastos, Maria Regina Reis Amendoeira
The aim of the present study was to evaluate the growth rate of Balantidium coli in three xenic media cultures. Between 2013 and 2015, 10 B. coli isolates obtained from feces of Cynomolgus macaques, and 30 isolates from feces of pigs were studied. An inoculum of 500 trophozoites was transferred to tubes containing LES, TYSGM-9 and Pavlova media. These cultures were evaluated at incubation times of 24, 48, 72 and 96 hours. In most of strains analyzed wasn't showed significant difference in the growth rate comparing TYSGM-9 and Pavlova media (Wilcoxon p>0...
February 19, 2018: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/29464928/metabolic-responses-and-pathway-changes-of-mammalian-cells-under-different-culture-conditions-with-media-supplementations
#10
Seo-Young Park, Thomas M Reimonn, Cyrus Agarabi, Kurt Brorson, Seongkyu Yoon
Amino acids and glucose consumption, cell growth and monoclonal antibody (mAb) production in mammalian cell culture are key considerations during upstream process and particularly media optimization. Understanding the interrelations and the relevant cellular physiology will provide insight for setting strategy of robust and effective mAb production. The aim of this study was to further our understanding of nutrient consumption metabolism, since this could have significant impact on enhancing mAb titer, cell proliferation, designing feeding strategies, and development of feed media...
February 21, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29464078/effects-of-a-small-molecule-r-spondin-1-substitute-rs-246204-on-a-mouse-intestinal-organoid-culture
#11
Myeong-Ok Nam, Soojung Hahn, Joo Hyun Jee, Tae-Sun Hwang, Ho Yoon, Dong Hyeon Lee, Min-Soo Kwon, Jongman Yoo
Organoids, a multi-cellular and organ-like structure cultured in vitro , can be used in a variety of fields such as disease modeling, drug discovery, or cell therapy development. When organoids derived from Lgr5 stem cells are cultured ex vivo , recombinant R-spondin-1 protein should be added at a high concentration for the initiation and maintenance of the organoids. Because the addition of large amounts of R-spondin-1 greatly increases the cost of organoids, the organoids grown with R-spondin-1 are not practical for large-scale drug screening and for the development of therapeutic agents...
January 19, 2018: Oncotarget
https://www.readbyqxmd.com/read/29463964/culturally-sensitive-chiropractic-care-of-the-transgender-community-a-narrative-review-of-the-literature
#12
REVIEW
Michele J Maiers, William K Foshee, Heidi Henson Dunlap
Objectives: Transgender individuals commonly experience barriers to quality health care and may suffer from unique musculoskeletal complaints. Although these needs are often inadequately addressed within the health care system, they could be attended to by the chiropractic community. This narrative review describes best practices for delivering culturally sensitive care to transgender patients within the context of chiropractic offices. Methods: A literature search generated peer-reviewed material on culturally competent care of the transgender community...
December 2017: Journal of Chiropractic Humanities
https://www.readbyqxmd.com/read/29463727/the-secreted-metabolome-of-streptomyces-chartreusis-and-implications-for-bacterial-chemistry
#13
Christoph H R Senges, Arwa Al-Dilaimi, Douglas H Marchbank, Daniel Wibberg, Anika Winkler, Brad Haltli, Minou Nowrousian, Jörn Kalinowski, Russell G Kerr, Julia E Bandow
Actinomycetes are known for producing diverse secondary metabolites. Combining genomics with untargeted data-dependent tandem MS and molecular networking, we characterized the secreted metabolome of the tunicamycin producer Streptomyces chartreusis NRRL 3882. The genome harbors 128 predicted biosynthetic gene clusters. We detected >1,000 distinct secreted metabolites in culture supernatants, only 22 of which were identified based on standards and public spectral libraries. S. chartreusis adapts the secreted metabolome to cultivation conditions...
February 20, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29462899/-i-wouldn-t-be-friends-with-someone-if-they-were-liking-too-much-rubbish-a-qualitative-study-of-alcohol-brands-youth-identity-and-social-media
#14
Richard I Purves, Martine Stead, Douglas Eadie
The consumption of alcohol by young people remains a major public health concern at both the national and international level. Levels of drinking among 15-yearolds in the United Kingdom (UK) remain significantly higher than the European average. This study explored how alcohol brands are used by young people to develop their desired identities and how these acts of consumption extend to young people's profiles on social media. It also deepens understanding of how alcohol brands are connected to young peoples' concerns about image and peer group dynamics...
February 16, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29462690/xpf-plays-an-indispensable-role-in-relieving-silver-nanoparticle-induced-dna-damage-stress-in-human-cells
#15
Dan Wang, Huali Yang, Zheng Zhou, Man Zhao, Runsheng Chen, Simon H Reed
Due to the specific antimicrobial activity of silver nanoparticles (AgNPs), they are widely used in wound dressings, coatings in medical devices and household products. In spite of the well-documented genotoxicity of AgNPs, the molecular mechanisms of relieving AgNP-induced DNA damage stress remain poorly understood. We report here that one of the DNA repair factors, XPF, plays a crucial role in resisting AgNP-induced DNA damage stress in human cells. Following culture with AgNP-containing media, severely decreased colony forming abilities have been observed in XPF mutant and knockdown cells compared with wild type or control cells respectively, demonstrating that XPF is required to resist the AgNP-induced stress...
February 17, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29462570/maternal-perceptions-of-infant-feeding-and-health-in-the-context-of-the-2015-nepal-earthquake
#16
Sarah DeYoung, Manoj Suji, Hannah G Southall
BACKGROUND: Displacement caused by the 2015 earthquake in Nepal exacerbated poor health and nutrition for thousands of women and children. Research aim: This study aimed to identify the perceptions of Nepalese mothers residing in earthquake relocation camps regarding barriers, facilitators, and situational factors influencing breastfeeding and family well-being. METHODS: An exploratory, rapid ethnographic approach was used during two fieldwork phases. Phase 1 consisted of reconnaissance and observation, whereas Phase 2 consisted of observation and qualitative semistructured interviews with infant caretakers ( N = 14)...
February 1, 2018: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/29460206/deciphering-the-trophic-interaction-between-akkermansia-muciniphila-and-the-butyrogenic-gut-commensal-anaerostipes-caccae-using-a-metatranscriptomic-approach
#17
Loo Wee Chia, Bastian V H Hornung, Steven Aalvink, Peter J Schaap, Willem M de Vos, Jan Knol, Clara Belzer
Host glycans are paramount in regulating the symbiotic relationship between humans and their gut bacteria. The constant flux of host-secreted mucin at the mucosal layer creates a steady niche for bacterial colonization. Mucin degradation by keystone species subsequently shapes the microbial community. This study investigated the transcriptional response during mucin-driven trophic interaction between the specialised mucin-degrader Akkermansia muciniphila and a butyrogenic gut commensal Anaerostipes caccae. A...
February 19, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/29459851/dynamics-of-metabolite-induction-in-fungal-co-cultures-by-metabolomics-at-both-volatile-and-non-volatile-levels
#18
Antonio Azzollini, Lorenzo Boggia, Julien Boccard, Barbara Sgorbini, Nicole Lecoultre, Pierre-Marie Allard, Patrizia Rubiolo, Serge Rudaz, Katia Gindro, Carlo Bicchi, Jean-Luc Wolfender
Fungal co-cultivation has emerged as a promising way for activating cryptic biosynthetic pathways and discovering novel antimicrobial metabolites. For the success of such studies, a key element remains the development of standardized co-cultivation methods compatible with high-throughput analytical procedures. To efficiently highlight induction processes, it is crucial to acquire a holistic view of intermicrobial communication at the molecular level. To tackle this issue, a strategy was developed based on the miniaturization of fungal cultures that allows for a concomitant survey of induction phenomena in volatile and non-volatile metabolomes...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29459640/self-assembled-collagen-fibrin-hydrogel-reinforces-tissue-engineered-adventitia-vessels-seeded-with-human-fibroblasts
#19
Bijal Patel, Zhengfan Xu, Cameron B Pinnock, Loay S Kabbani, Mai T Lam
Efforts for tissue engineering vascular grafts focuses on the tunica media and intima, although the tunica adventitia serves as the primary structural support for blood vessels. In surgery, during endarterectomies, surgeons can strip the vessel, leaving the adventitia as the main strength layer to close the vessel. Here, we adapted our recently developed technique of forming vascular tissue rings then stacking the rings into a tubular structure, to accommodate human fibroblasts to create adventitia vessels in 8 days...
February 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29458862/comparative-transcriptomic-analysis-of-cerrena-unicolor-revealed-differential-expression-of-genes-engaged-in-degradation-of-various-kinds-of-wood
#20
Grzegorz Janusz, Andrzej Mazur, Jerzy Wielbo, Piotr Koper, Kamil Żebracki, Anna Pawlik, Beata Ciołek, Andrzej Paszczyński, Agnieszka Kubik-Komar
To explore the number of enzymes engaged by Cerrena unicolor FCL139 for wood degradation, the transcriptomes of the fungus growing on birch, ash, maple sawdust and the control liquid medium were analyzed. Among 12,966 gene models predicted for the C. unicolor genome, 10,396 all-unigenes were detected, of which 9567 were found to be expressed in each of the tested growth media. The highest number (107) of unique transcripts was detected during fungus growth in the control liquid medium, while the lowest number (11) - in the fungal culture comprising maple saw dust...
March 2018: Microbiological Research
keyword
keyword
95742
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"