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https://www.readbyqxmd.com/read/28342961/particulate-metal-bioaccessibility-in-physiological-fluids-and-cell-culture-media-toxicological-perspectives
#1
Bérénice Leclercq, Laurent Yves Alleman, Esperanza Perdrix, Véronique Riffault, Mélanie Happillon, Alain Strecker, Jean-Marc Lo-Guidice, Guillaume Garçon, Patrice Coddeville
According to the literature, tiny amounts of transition metals in airborne fine particles (PM2.5) may induce proinflammatory cell response through reactive oxygen species production. The solubility of particle-bound metals in physiological fluids, i.e. the metal bioaccessibility is driven by factors such as the solution chemical composition, the contact time with the particles, and the solid-to-liquid phase ratio (S/L). In this work, PM2.5-bound metal bioaccessibility was assessed in various physiological-like solutions including cell culture media in order to evidence the potential impact on normal human bronchial epithelial cells (NHBE) when studying the cytotoxicity and inflammatory responses of PM2...
March 23, 2017: Environmental Research
https://www.readbyqxmd.com/read/28342852/effects-of-sub-culturing-on-genetic-and-physiological-parameters-in-different-beauveria-bassiana-isolates
#2
Naser Eivazian Kary, Zhila Alizadeh
Beauveria bassiana is a fungus which is widely used as a biological insecticide to control a number of economically important insect pests. Knowledge of the genetic diversity of the isolates, understanding the underlying nature of these evolutionary phenomena and finding appropriate and simple screening tools play an important role in developing effective biocontrol agents. Here, we monitored changes of electrophoretic karyotype of small molecules of extrachromosomal DNAs, presumably mitochondrial DNA or plasmids in several individual isolates of B...
March 22, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28342745/culture-of-non-typeable-haemophilus-influenzae-from-the-nasopharynx-not-all-media-are-equal
#3
Tegan M Harris, Angela Rumaseb, Jemima Beissbarth, Federica Barzi, Amanda J Leach, Heidi C Smith-Vaughan
The efficacy of chocolate agar, versus bacitracin, vancomycin, clindamycin, chocolate agar (BVCCA) for the isolation of non-typeable Haemophilus influenzae (NTHi) from nasopharyngeal swabs was determined. BVCCA cultured NTHi from 97.3% of NTHi-positive swabs, compared to 87.1% for chocolate agar. To maximise culture sensitivity, the use of both media is recommended.
March 22, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28339090/inhibition-of-farnesyl-pyrophosphate-synthase-attenuates-high-glucose%C3%A2-induced-vascular-smooth-muscle-cells-proliferation
#4
Guo-Ping Chen, Xiao-Qin Zhang, Tao Wu, Jie Han, Dan Ye
The proliferation of vascular smooth muscle cells (VSMCs) is one of the main features of atherosclerosis accelerated by hyperglycemia. Our previous studies found that farnesyl pyrophosphate synthase (FPPS, EC 2.5.1.10), an essential enzyme in the mevalonate pathway, was upregulated in aorta media from diabetic mice along with the process of atherosclerosis. However, the exact role of FPPS in high glucose‑induced proliferation of VSMCs is largely unclear. In our study, we found that alendronate (an FPPS inhibitor) attenuated diabetic accelerated atherosclerosis in vivo and suppressed high glucose‑induced VSMCs proliferation in vitro...
March 23, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28339035/duration-of-simulated-microgravity-affects-the-differentiation-of-mesenchymal-stem-cells
#5
Li Xue, Yaohui Li, Jun Chen
Previous evidence has suggested that physical microenvironments and mechanical stresses, independent of soluble factors, influence mesenchymal stem cell (MSC) fate. In the present study, simulated microgravity (SMG) was demonstrated to regulate the differentiation of mesenchymal stem cells. This may be a novel strategy for tissue engineering and regenerative medicine. Rat MSCs were cultured for 72 h or 10 days in either normal gravity or a clinostat to model microgravity, followed with culture in diverse differential media...
March 22, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28338443/fluorescent-labeling-of-collagen-production-by-cells-for-noninvasive-imaging-of-extracellular-matrix-deposition
#6
Katie Bardsley, Ying Yang, Alicia J El Haj
Extracellular matrix (ECM) is an essential component of tissues and provides both integrity and biological cues for cells. Collagen is one of the major proteins found within the ECM and therefore is an essential component of all engineered tissues. Therefore, in this article, we present a method for the online real-time monitoring of collagen deposition in three-dimensional engineered constructs. This method revolves around modification of collagen through the addition of azide-L-proline to cell culture media...
March 24, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28337800/mycoplasma-infection-of-the-middle-ear-in-three-cats
#7
Amanda L Ackermann, Jennifer Ann Lenz, Elizabeth R May, Linda A Frank
BACKGROUND: Mycoplasma spp. are commensal organisms found in association with the mucus membranes of all mammalian species and are implicated in bacterial infections of many different locations. Mycoplasma spp. as a primary pathogen associated with otitis media in cats has not been reported. OBJECTIVES: To describe three cats with Mycoplasma infection of the middle ear associated with various underlying disease processes. ANIMALS: Three client-owned cats...
March 23, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28337288/which-has-more-stem-cell-characteristics-m%C3%A3-ller-cells-or-m%C3%A3-ller-cells-derived-from-in-vivo-culture-in-neurospheres
#8
Hong-Pei Ji, Yu Xiong, En-Dong Zhang, Wei-Tao Song, Zhao-Lin Gao, Fei Yao, Hong Sun, Rong-Rong Zhou, Xiao-Bo Xia
OBJECTIVE: Müller cells can be acquired from in vitro culture or a neurosphere culture system. Both culture methods yield cells with progenitor-cell characteristics that can differentiate into mature nervous cells. We compared the progenitor-cell traits of Müller cells acquired from each method. METHODS: Primary murine Müller cells were isolated in serum culture media and used to generate Müller cells derived from neurospheres in serum-free culture conditions...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28336890/in-vitro-comparative-skin-irritation-induced-by-nano-and-non-nano-zinc-oxide
#9
Maria Pilar Vinardell, Hector Llanas, Laura Marics, Montserrat Mitjans
This study was designed to determine whether nano-sized ZnO has the potential to cause acute cutaneous irritation using cultured HaCaT keratinocytes and a human skin equivalent as in vitro models, compared to non-nanomaterials. Commercial nano ZnO with different sizes (50 nm and 100 nm) was characterized by dynamic light scattering (DLS) and microscopy (SEM) in different media. Nano ZnO reduced the cell viability of HaCaT in a dose-dependent and time-dependent manner, in a similar way to macro ZnO. However, the 3D-epidermis model revealed no irritation at 1 mg/mL after 24 h of exposure...
March 4, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28336163/a-meta-analysis-of-the-impact-of-human-leukocyte-antigen-g-on-the-outcomes-of-ivf-icsi
#10
Ziru Niu, Liangyi Wang, Ronald T K Pang, Yifan Guo, William S B Yeung, Yuanqing Yao
This analysis was performed to determine whether the presence of soluble human leukocyte antigen-G (sHLA-G) in embryo culture medium is predictive of clinical outcomes in IVF treatment. The outcomes of implantation, clinical pregnancy, multiple pregnancy and miscarriage, between groups with and without sHLA-G in embryo culture media, were analysed. Fifteen studies with a total of 6170 cases were included. Ten of them were prospective studies while five were retrospective studies. Embryo culture media with sHLA-G were associated with significantly higher implantation rate and clinical pregnancy rate when compared with those without; the odd ratios (ORs) were 2...
March 14, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28334549/limitations-of-immunomagnetic-separation-for-detection-of-the-top-seven-serogroups-of-shiga-toxin-producing-escherichia-coli
#11
J Hallewell, T Alexander, T Reuter, K Stanford
Shiga toxin-producing Escherichia coli (STEC) strains are foodborne pathogens that negatively impact human health and compromise food safety. Serogroup O157 is the most frequently isolated and studied STEC serogroup, but six others (O26, O45, O103, O111, O121, and O145) have also been identified as significant sources of human disease and collectively have been referred to as the "top six" pathogenic serogroups. Because detection methods for non-O157 serogroups are not yet refined, the objective of this study was to compare the effectiveness of immunomagnetic separation (IMS) for recovery of serogroup O157 isolates with that for each of the top six E...
March 9, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28334088/relapse-re-infection-and-mixed-infections-in-tuberculosis-disease
#12
Amanda McIvor, Hendrik Koornhof, Bavesh Davandra Kana
Tuberculosis (TB) disease can be characterized by genotypic and phenotypic complexity in Mycobacterium tuberculosis bacilli within a single patient. This microbiological heterogeneity has become an area of intense study due its perceived importance in drug tolerance, drug resistance and as a surrogate measure of transmission rates. This review presents a descriptive analysis of research describing the prevalence of mixed strain TB infections in geographically distinct locations. Despite significant variation in disease burden and a rampant HIV-TB co-epidemic, there was no difference in the prevalence range of mixed infections reported in African countries when compared to the rest of the world...
February 18, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28333084/establishing-the-secondary-metabolite-profile-of-the-marine-fungus-tolypocladium-geodes-sp-mf458-and-subsequent-optimisation-of-bioactive-secondary-metabolite-production
#13
Bethlehem Kebede, Stephen K Wrigley, Anjali Prashar, Janina Rahlff, Markus Wolf, Jeanette Reinshagen, Philip Gribbon, Johannes F Imhoff, Johanna Silber, Antje Labes, Bernhard Ellinger
As part of an international research project, the marine fungal strain collection of the Helmholtz Centre for Ocean Research (GEOMAR) research centre was analysed for secondary metabolite profiles associated with anticancer activity. Strain MF458 was identified as Tolypocladium geodes, by internal transcribed spacer region (ITS) sequence similarity and its natural product production profile. By using five different media in two conditions and two time points, we were able to identify eight natural products produced by MF458...
March 23, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28332167/controlled-release-of-vanadium-from-a-composite-scaffold-stimulates-mesenchymal-stem-cell-osteochondrogenesis
#14
S D Schussler, K Uske, P Marwah, F W Kemp, J D Bogden, S S Lin, Treena Livingston Arinzeh
Large bone defects often require the use of autograft, allograft, or synthetic bone graft augmentation; however, these treatments can result in delayed osseous integration. A tissue engineering strategy would be the use of a scaffold that could promote the normal fracture healing process of endochondral ossification, where an intermediate cartilage phase is later transformed to bone. This study investigated vanadyl acetylacetonate (VAC), an insulin mimetic, combined with a fibrous composite scaffold, consisting of polycaprolactone with nanoparticles of hydroxyapatite and beta-tricalcium phosphate, as a potential bone tissue engineering scaffold...
March 22, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28332163/fermentation-of-dietetic-fiber-from-green-bean-and-prickly-pear-shell-by-pure-and-mixture-culture-of-lactobacillus-acidophilus-la-5-and-bifidobacterium-bifidum-450b
#15
Y N Mora-Cura, N P Meléndez-Rentería, M Delgado-García, J C Contreras-Esquivel, J A Morlett-Chávez, C N Aguilar, R Rodríguez-Herrera
The aim of this study was to evaluate the fermentation of dietary fiber from green bean (Phaseolus vulgaris) and prickly pear shell (Opuntia ficus-indica) by Lactobacillus acidophilus LA-5 and Bifidobacterium bifidum 450B growing as mono-culture and co-culture, the fermentation products, and proteins expressed during this process. The analysis of the fermentation profile showed a major growth of bacteria in the culture media of each dietary fiber supplemented with glucose, and particularly B. bifidum 450B at 48 h showed the highest growth...
March 23, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28331545/metabolic-engineering-of-saccharomyces-cerevisiae-to-produce-a-reduced-viscosity-oil-from-lignocellulose
#16
Tam N T Tran, Rebecca J Breuer, Ragothaman Avanasi Narasimhan, Lucas S Parreiras, Yaoping Zhang, Trey K Sato, Timothy P Durrett
BACKGROUND: Acetyl-triacylglycerols (acetyl-TAGs) are unusual triacylglycerol (TAG) molecules that contain an sn-3 acetate group. Compared to typical triacylglycerol molecules (here referred to as long chain TAGs; lcTAGs), acetyl-TAGs possess reduced viscosity and improved cold temperature properties, which may allow direct use as a drop-in diesel fuel. Their different chemical and physical properties also make acetyl-TAGs useful for other applications such as lubricants and plasticizers...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28330280/culturable-endophytic-bacteria-associated-with-medicinal-plant-ferula-songorica-molecular-phylogeny-distribution-and-screening-for-industrially-important-traits
#17
Yong-Hong Liu, Jian-Wei Guo, Nimaichand Salam, Li Li, Yong-Guang Zhang, Jian Han, Osama Abdalla Mohamad, Wen-Jun Li
Xinjiang, a region of high salinity and drought, is a host to many arid and semi-arid plants. Many of these plants including Ferula spp. have indigenous pharmaceutical histories. As many of the medicinal properties of plants are in tandem with the associated microorganisms residing within the plant tissues, it is advisable to explore the endophytic potential of such plants. In the present study, diversity of culturable bacteria isolated from medicinal plants Ferula songorica collected from Hebukesaier, Xinjiang were analyzed...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330278/effects-of-the-culture-media-optimization-on-pectinase-production-by-enterobacter-sp-pstb-1
#18
M Purna Chandra Reddy, K V Saritha
In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330254/enhanced-production-of-an-anti-malarial-compound-artesunate-by-hairy-root-cultures-and-phytochemical-analysis-of-artemisia-pallens-wall
#19
Zarna Pala, Vishnu Shukla, Anshu Alok, Subhash Kudale, Neetin Desai
Artemisinin and its derivatives are still one of the most effective drugs for the treatment of malaria. Artemisia pallens commonly known as Dhavanam, is an aromatic herb belonging to the family Asteraceae. Increasing the artemisinin content of A. pallens by genetic engineering would improve the availability of this much needed drug. In the present study, Agrobacterium rhizogenes (strain NCIM 5140) mediated genetic transformation of Artemisia pallens were carried out for hairy root induction. The effect of different media (Half MS, MS, MS along with BAP 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330244/elucidation-of-impact-of-heavy-metal-pollution-on-soil-bacterial-growth-and-extracellular-polymeric-substances-flexibility
#20
Muniswamy David, Paidi Murali Krishna, Jeybalan Sangeetha
Metal bioaccessibility is an alarming issue in croplands of mining sites due to overloading of toxic metals. Hence, the present study is aimed to determine the overloading of toxic metal in croplands across the Tawag village, Hutti, Raichur, India. Correspondingly, to identify the soil bacterial growth, physiological oxidative stress enzyme activity and surface macromolecular functional group evolution were analysed in and around the toxic metal contaminated sites through FT-IR and FT-Raman spectrometry. The evaluated results attribute that the study area is heavily polluted with the toxic metals such as arsenic, cadmium, chromium, lead and zinc...
December 2016: 3 Biotech
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