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https://www.readbyqxmd.com/read/29915432/%C3%AF-editors-choice%C3%AF-effects-of-exosomes-derived-from-the-induced-pluripotent-stem-cells-on-skin-wound-healing
#1
Hitoshi Kobayashi, Katsumi Ebisawa, Miki Kambe, Takatoshi Kasai, Hidetaka Suga, Kae Nakamura, Yuji Narita, Aika Ogata, Yuzuru Kamei
Recently, the effects of stem cell supernatants or exosomes, such as skin wounds, have attracted attention. However, the effects of the induced pluripotent stem (iPS) cell-derived exosomes (iPS-Exos) have not been investigated in detail. Here, we investigated the effects of iPS-Exos on skin wound healing using an animal model. We isolated iPS-Exos from the iPS cell culture media. Control exosomes were isolated from unused iPS cell culture media (M-Exos). We first observed the morphologic characteristics of the isolated exosomes and examined the expression of surface antigens...
May 2018: Nagoya Journal of Medical Science
https://www.readbyqxmd.com/read/29914830/clinical-factors-that-influence-the-cellular-responses-of-saphenous-veins-used-for-arterial-bypass
#2
Michael Sobel, Shinsuke Kikuchi, Lihua Chen, Gale L Tang, Tom N Wight, Richard D Kenagy
OBJECTIVE: When an autogenous vein is harvested and used for arterial bypass, it suffers physical and biologic injuries that may set in motion the cellular processes that lead to wall thickening, fibrosis, stenosis, and ultimately graft failure. Whereas the injurious effects of surgical preparation of the vein conduit have been extensively studied, little is known about the influence of the clinical environment of the donor leg from which the vein is obtained. METHODS: We studied the cellular responses of fresh saphenous vein samples obtained before implantation in 46 patients undergoing elective lower extremity bypass surgery...
June 15, 2018: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29914798/neuregulin-1-regulates-amyloid-precursor-protein-cell-surface-expression-and-non-amyloidogenic-processing
#3
Young-Jung Kim, Ji-Young Yoo, Ok-Soon Kim, Han-Byeol Kim, Junghwa Ryu, Hye-Sun Kim, Jun-Ho Lee, Hong-Il Yoo, Dae-Yong Song, Tai-Kyoung Baik, Ran-Sook Woo
The amyloid precursor protein (APP) is a key molecule in Alzheimer's disease. The prevailing view is that APP is initially transported to the plasma membrane as a full-length protein. Its localization at the cell surface can trigger downstream signaling and APP cleavage. Our previous work has shown that Neuregulin 1 (NRG1) has neuroprotective effects in an Alzheimer's disease model. In the present study, we examine whether NRG1 signaling is involved in APP expression and non-amyloidogenic processing in neuronal cells...
May 29, 2018: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/29913575/inequality-in-access-to-cultural-ecosystem-services-from-protected-areas-in-the-chilean-biodiversity-hotspot
#4
Maria Jose Martinez-Harms, Brett A Bryan, Spencer A Wood, David M Fisher, Elizabeth Law, Jonathan R Rhodes, Cynnamon Dobbs, Duan Biggs, Kerrie A Wilson
Experiences with nature through visits to protected areas provide important cultural ecosystem services that have the potential to strengthen pro-environmental attitudes and behavior. Understanding accessibility to protected areas and likely preferences for enjoying the benefits of nature visits are key factors in identifying ways to reduce inequality in access and inform the planning and management for future protected areas. We develop, at a regional scale, a novel social media database of visits to public protected areas in part of the Chilean biodiversity hotspot using geotagged photographs and assess the inequality of access using the home locations of the visitors and socio-economic data...
September 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29913423/effect-of-aminopeptidase-n-on-functions-and-fertility-of-mouse-spermatozoa-in-vitro
#5
Amena Khatun, Kyu-Ho Kang, Do-Yeal Ryu, Md Saidur Rahman, Woo-Sung Kwon, Myung-Geol Pang
Aminopeptidase N (APN) is defined as a multifunctional enzyme, which regulate cellular physiology of a wide variety of cells in human. Earlier studies reported that mammalian semen shares this common enzyme as a major protein of seminal plasma that has correlation with male fertility, while the regulatory mechanisms of APN in spermatozoa are still far from being well understood. Present study was designed to investigate the role of APN in biological and chemical functions of spermatozoa using an in vitro antagonistic approach...
June 12, 2018: Theriogenology
https://www.readbyqxmd.com/read/29912910/impact-of-delipidated-estrous-sheep-serum-supplementation-on-in-vitro-maturation-cryotolerance-and-endoplasmic-reticulum-stress-gene-expression-of-sheep-oocytes
#6
Natalibeth Barrera, Pedro C Dos Santos Neto, Federico Cuadro, Diego Bosolasco, Ana P Mulet, Martina Crispo, Alejo Menchaca
High lipid content of oocytes and embryos in domestic animals is one of the well-known factors associated with poor cryosurvival. Herein, we wanted to determine whether the use of delipidated estrous sheep serum during in vitro maturation (IVM) of ovine oocytes reduces the cytoplasmic lipid droplets content and improves embryo development and cryotolerance after vitrification. Cumulus oocytes complexes (COCs) were matured in vitro for 24 h in medium supplemented with whole or delipidated estrous sheep serum prior to vitrification...
2018: PloS One
https://www.readbyqxmd.com/read/29912907/evaluation-of-the-genexpert-mtb-rif-in-patients-with-presumptive-tuberculous-meningitis
#7
Tatiana Metcalf, Jaime Soria, Silvia M Montano, Eduardo Ticona, Carlton A Evans, Luz Huaroto, Matthew Kasper, Eric S Ramos, Nicanor Mori, Podjanee Jittamala, Kesinee Chotivanich, Irwin F Chavez, Pratap Singhasivanon, Sasithon Pukrittayakamee, Joseph R Zunt
BACKGROUND: Meningitis caused by Mycobacterium tuberculosis is a major cause of morbidity and mortality worldwide. We evaluated the performance of cerebrospinal fluid (CSF) testing with the GeneXpert MTB/RIF assay versus traditional approaches for diagnosing tuberculosis meningitis (TBM). METHODS: Patients were adults (n = 37) presenting with suspected TBM to the Hospital Nacional Dos de Mayo, Lima, Peru, during 12 months until 1st January 2015. Each participant had a single CSF specimen that was divided into aliquots that were concurrently tested for M...
2018: PloS One
https://www.readbyqxmd.com/read/29911500/muslim-immigration-critical-events-and-the-seeds-of-majority-members-support-for-radical-responses-an-interactionist-perspective
#8
Stefan Stürmer, Anette Rohmann, Laura Froehlich, Jolanda van der Noll
This article uses an interactionist perspective to understand the role of media framings of critical events in catalyzing Western citizens' support for radical responses to Muslim immigration (e.g., armed self-defense). A multi-method series of three studies tested this perspective in the context of the 2015/2016 Cologne New Year's Eve sexual assaults on women. Study 1, a content analysis of 163 online newspaper articles, revealed that mass media attributed the assaults to the suspects' Muslim culture. Study 2, a correlational study ( N = 487) conducted at the peak of the media coverage, confirmed that the degree to which participants accepted the veracity of the culture-focused media representation strengthened the relation between their feelings of symbolic threat as a result of Muslim immigration and their approval of radical responses...
June 1, 2018: Personality & Social Psychology Bulletin
https://www.readbyqxmd.com/read/29910684/improvement-of-animal-feed-additives-of-ginkgo-leaves-through-solid-state-fermentation-using-aspergillus-niger
#9
Jiahong Wang, Fuliang Cao, Erzheng Su, Linguo Zhao, Wensheng Qin
To improve the quality of Ginkgo biloba leaves as biological feed additives, twelve Aspergillus niger strains were evaluated for their growth in the moisture ginkgo leaf meal media through solid-state fermentation . The results relating to flavor, flavonoids, enzymes, crude protein, and reducing sugars showed A. niger Gyx086 strain was capable of efficiently fermenting ginkgo leaves. The optimal cultural conditions were three loops of spores inoculation to every 75 g medium containing 60 % water, grew at 28˚C for 48 h...
2018: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29910677/propofol-attenuates-osteoclastogenesis-by-lowering-rankl-opg-ratio-in-mouse-osteoblasts
#10
Do-Won Lee, Jae-Young Kwon, Hae-Kyu Kim, Hyeon-Jeong Lee, Eun-Soo Kim, Hyae-Jin Kim, Hyung-Joon Kim, Han-Bit Lee
Bone remodeling plays an important role in the bone healing process; for example, following fracture. The relative ratio of the receptor activator of nuclear factor kappa B ligand (RANKL)/ osteoprotegerin (OPG) controls osteoclast differentiation, thereby playing a pivotal role in the regulation of bone remodeling. Propofol, a widely used anesthetic agent in orthopedic procedures, is considered to possess potential antioxidant properties owing to its structural similarity to α-tocopherol. Antioxidants are known to enhance bone healing...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29910353/perceptions-and-determinants-of-eating-for-health-and-performance-in-high-level-male-adolescent-rugby-union-players
#11
Emily G Stokes, Roger Hughes, David M Shaw, Helen T O'Connor, Kathryn L Beck
Sports nutrition recommendations provide guidance on dietary strategies to optimise sports performance. However, research indicates that young athletes often find it difficult to follow these guidelines in practice. Limited research exists on the determinants that influence adherence to sports nutrition guidelines. This study aimed to explore the perceptions and determinants of eating for health and performance in high-level male adolescent rugby union players. Determinants were explored using semi-structured individual interviews in New Zealand high-level male rugby union players ( n = 20, 16⁻18 years)...
May 28, 2018: Sports
https://www.readbyqxmd.com/read/29908567/construction-of-an-improved-aspergillus-niger-platform-for-enhanced-glucoamylase-secretion
#12
Markus R M Fiedler, Lars Barthel, Christin Kubisch, Corrado Nai, Vera Meyer
BACKGROUND: The lifestyle of filamentous fungi depends on the secretion of hydrolytic enzymes into the surrounding medium, which degrade polymeric substances into monomers that are then taken up to sustain metabolism. This feature has been exploited in biotechnology to establish platform strains with high secretory capacity including Aspergillus niger. The accepted paradigm is that proteins become mainly secreted at the tips of fungal hyphae. However, it is still a matter of debate if the amount of growing hyphal tips in filamentous fungi correlates with an increase in secretion, with previous studies showing either a positive or no correlation...
June 16, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29908546/sublingual-immunotherapy-with-sal-k1-expressing-lactococcus-lactis-down-regulates-th2-immune-responses-in-balb-c-mice
#13
Ziba Ghasemi, Abdol-Reza Varasteh, Malihe Moghadam, Seyed-Amir Jalali, Ali Anissian, Mojtaba Sankian
Sublingual immunotherapy (SLIT) has been introduced as a noninvasive and safer approach for allergen-specific immunotherapies. In this study we investigated the efficacy of oral immunotherapy with recombinant Salsola kali 1 protein (Sal k 1) on Th1/Th2 balance in a mouse model of allergy. Female Balb/c mice were intraperitoneally sensitized with rSal k1, followed by a respiratory challenge with 1% (w/v) rSal k1. The sensitized mice were subjected to SLIT using rSal K1 expressing Lactobacillus lactis strain for three weeks...
June 2018: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29908408/the-interplay-between-light-plant-growth-regulators-and-elicitors-on-growth-and-secondary-metabolism-in-cell-cultures-of-fagonia-indica
#14
Tariq Khan, Bilal Haider Abbasi, Mubarak Ali Khan
Manipulation in the light regimes combined with the effects of plant growth regulators (PGRs) and elicitors through plant cell culture technology is a promising strategy for enhancing the yield of medicinally important secondary metabolites. In this study, the effects of interplay between PGRs, elicitors and light regimes on cell cultures of F. indica have been investigated. The results showed that callus cultures resulted in maximum biomass formation (13.2 g/L) when incubated on solid MS (Murashige and Skoog) medium containing 1...
June 9, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29908315/expression-and-purification-of-pneumococcal-surface-protein-a-of-clade-4-in-escherichia-coli-using-hydroxylapatite-and-ion-exchange-column-chromatography
#15
Hualong Xi, Jinfei Yu, Qing Sun, Jingcai Lu, Tiejun Gu, Xiaonan Guo, Bo Li, Xiaorui Chen, Kaixin Zhang, Wei Kong, Yongge Wu
Streptococcus pneumoniae is a major pathogen that causes life-threatening diseases, such as pneumonia, otitis media, bacteremia, and meningitis, worldwide and especially in young children and the elderly. Pneumococcal surface protein A (PspA) is a widely studied candidate protein vaccine that represents a promising replacement for current polysaccharide and polysaccharide-conjugate vaccines. In this study, we describe a simple method to produce PspA of clade 4 from an Escherichia coli expression system using hydroxylapatite and ion-exchange chromatography...
June 13, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29908269/the-effect-of-zataria-multiflora-boiss-essential-oil-on-the-growth-and-citrinin-production-of-penicillium-citrinum-in-culture-media-and-cheese
#16
Fateme Akrami Mohajeri, Ali Misaghi, Hamidreza Gheisari, Afshin Akhondzadeh Basti, Assieh Amiri, Sayyed Razi Ghalebi, Zahra Derakhshan, Roohollah Dehghani Tafti
The effect of Zataria multiflora Boiss Essential oil (EO) on the growth, spore production, and citrinin production of Penicellium citrinum PTCC 5304 in the culture media as well as Iranian ultra-filtered white cheese in brine was investigated. Radial growth and spore production on the potato dextrose agar (PDA) were effectively inhibited by EO in a dose-dependent manner. At 200 ppm, the radial growth and sporulation declined by 92% and 100%, respectively. The growth was completely prevented at 400 ppm of EO on PDA and the minimum fungicidal concentration (MFC) of the oil was estimated at 400 ppm...
June 13, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29908000/serpin-a1-and-the-modulation-of-type-i-collagen-turnover-effect-of-the-c-terminal-peptide-409-418-sa1-iii-in-human-dermal-fibroblasts
#17
Caterina Cipriani, Simona Pascarella, Fosca Errante, Beatrice Menicacci, Lucia Magnelli, Alessandra Mocali, Paolo Rovero, Lisa Giovannelli
The pharmacological modulation of collagen turnover is a strategy potentially useful in different skin conditions. The serine protease inhibitor Serpin A1 and portions of its C-terminal region have been investigated as collagen modulators. To clarify the mechanisms by which the C-terminal 409-418 peptide SA1-III increases extracellular type I collagen levels, to compare its activities range with that of the originator molecule Serpin A1, and to evaluate its efficacy in primary cultures from adult and aged human subjects...
June 16, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29907186/isolation-of-naturally-infecting-leishmania-infantum-from-canine-samples-in-novy-macneal-nicolle-medium-prepared-with-defibrinated-blood-from-different-animal-species
#18
Roseclea Chagas Dos Santos, Flaviane Alves de Pinho, Gabriela Porfírio Passos, Daniela Farias Larangeira, Stella Maria Barrouin-Melo
The most commonly used culture medium for the in vitro isolation of Leishmania spp. from canine biological samples is biphasic Novy-MacNeal-Nicolle (NNN) medium, whose solid phase is prepared using rabbit blood. Leishmania infantum parasites from natural infections are highly sensitive and demanding for growth in axenic conditions when firstly obtained from the dog's body. The objective of this study was to evaluate whether NNN medium (NNN-test) prepared with chicken blood (NNN-C), ox blood (NNN-O), horse blood (NNN-H) or sheep blood (NNN-S) was viable for the isolation of parasites from naturally infected dogs, in an endemic area for visceral leishmaniasis caused by L...
June 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29906703/modulation-of-serum-albumin-protein-corona-for-exploring-cellular-behaviors-of-fattigation-platform-nanoparticles
#19
Van Hong Nguyen, Nilesh M Meghani, Hardik H Amin, Thao T D Tran, Phuong H L Tran, Chulhun Park, Beom-Jin Lee
Albumin is the most abundant protein in blood, and is the most frequently identified protein in the protein corona of nanoparticles (NPs). Thus, albumin plays an important role in modulating NPs' physicochemical properties and bioavailability. In this study, the effect of bovine serum albumin (BSA) on gelatin-oleic nanoparticles' (GONs) physicochemical properties and cellular uptake were evaluated. Coumarin-6 was used as indicator to track the cellular uptake of GONs. The binding of BSA onto the GON surface increased the size, slightly reduced the negative net charge of the GON, and improved GON stability...
May 28, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29906256/isolation-of-colonization-defective-escherichia-coli-mutants-reveals-critical-requirement-for-fatty-acids-in-bacterial-colony-formation
#20
Kazuki Nosho, Koji Yasuhara, Yuto Ikehata, Tomohiro Mii, Taichiro Ishige, Shunsuke Yajima, Makoto Hidaka, Tetsuhiro Ogawa, Haruhiko Masaki
Most bacterial cells in nature exhibit extremely low colony-forming activity, despite showing various signs of viability, impeding the isolation and utilization of many bacterial resources. However, the general causes responsible for this state of low colony formation are largely unknown. Because liquid cultivation typically yields more bacterial cell cultures than traditional solid cultivation, we hypothesized that colony formation requires one or more specific gene functions that are dispensable or less important for growth in liquid media...
June 15, 2018: Microbiology
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