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https://www.readbyqxmd.com/read/29656470/mechanical-and-in-vitro-degradation-behavior-of-magnesium-bioactive-glass-composites-prepared-by-sps-for-biomedical-applications
#1
Sourav Dutta, K Bavya Devi, Sanjay Gupta, Biswanath Kundu, Vamsi Krishna Balla, Mangal Roy
In order to make magnesium (Mg) a successful candidate for fracture fixation devices, it is imperative to control the corrosion rate and enhance its elastic modulus. In the present work, we have prepared bioactive glass (BG) reinforced magnesium composite using spark plasma sintering (SPS). Simultaneous application of heat and pressure during SPS decreased the softening point of BG (600°C), allowing it to coat the Mg particles partially. As a result, BG was found along the Mg particle boundaries, which was confirmed by elemental mapping...
April 15, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29656182/co-culture-of-ovarian-cancer-stem-like-cells-with-macrophages-induced-skov3-cells-stemness-via-il-8-stat3-signaling
#2
Yingxia Ning, Yinghong Cui, Xiang Li, Xiaocheng Cao, A Chen, Chang Xu, Jianguo Cao, Xin Luo
Among recent concepts in the cancer biology field, the tumor microenvironment is highly associated with cancer stem cells, and plays a key role in tumor progression. This study aimed to explore the mechanism that the stemness induction of SKOV3 cell line by macrophages derived from THP-1 cells, which was co-cultured with SKOV3-derived ovarian cancer stem-like cells (OCSLCs). Sphere formation, soft agar colony formation, and expression levels of CD133 and CD44 were assessed to reflect OCSLC properties. ELISA was used to evaluate secretion profile changes in macrophages co-cultured with or without SKOV3-derived OCSLCs...
April 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29656164/lignolytic-mushroom-lenzites-elegans-wdp2-laccase-production-characterization-and-bioremediation-of-synthetic-dyes
#3
Raj Kumar Pandey, Salil Tewari, Lakshmi Tewari
A mycoremedial study was undertaken for decolourization of synthetic dyes using wood rot fungal culture Lenzites elegans WDP2. The culture was isolated from decaying wood as fruiting body, and identified on the basis of 5.8S ITS rRNA gene sequence analysis. Qualitative plate screening of culture showed extracellular laccase and lignin peroxidase production, while only laccase enzyme was produced in higher amount (156.793 Uml-1 ) in minimal salt broth medium containing glucose and veratryl alcohol. Laccase activity was increased up to 189...
April 12, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29655629/intramuscular-vaccination-of-guinea-pigs-with-the-live-attenuated-human-herpes-simplex-vaccine-vc2-stimulates-a-transcriptional-profile-of-vaginal-th17-and-regulatory-tr1-responses
#4
Brent A Stanfield, Paul J F Rider, John Caskey, Fabio Del Piero, Konstantin G Kousoulas
Herpes simplex virus is a common causative agent of oral and genital diseases. Novel vaccines and therapeutics are needed to combat herpes infections especially after the failure of subunit vaccines in human clinical trials. We have shown that the live-attenuated HSV-1 VC2 vaccine strain is unable to establish latency in vaccinated animals and produces a robust immune response capable of completely protecting mice against lethal vaginal HSV-1 or HSV-2 infections. The guinea pig represents the best small animal model of genital HSV-2 disease...
April 11, 2018: Vaccine
https://www.readbyqxmd.com/read/29655599/plasma-from-some-cancer-patients-inhibits-adenoviral-ad5f35-vector-transduction-of-dendritic-cells
#5
Ping Jin, Wenjing Chen, Jiaqiang Ren, Steven Chen, Lauren Wood, Yingdong Zhao, Alan Remaley, Chauha Pham, Sheena Lian, Shutong Liu, Hui Liu, Steven Highfill, Jay A Berzofsky, David F Stroncek
BACKGROUND: Pooled AB serum is often used as a media supplement for cell culture but it has the potential to transmit infectious diseases. To avoid this risk, we used autologous plasma as a media supplement for manufacturing dendritic cells (DCs) for cancer immunotherapy. We noticed inconsistencies in the DCs and investigated their nature and cause. METHODS: Adenovirus human epidural growth factor receptor 2 (adHER2/neu) DCs for 21 patients were manufactured from autologous peripheral blood monocytes that were treated with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 for 3 days, transduced with Ad5f35HER2ECTM and then treated with lipopolysaccharide and interferon (IFN)-γ for 1 day...
April 11, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29653286/stromal-cell-laden-3d-hydrogel-microwell-arrays-as-tumor-microenvironment-model-for-studying-stiffness-dependent-stromal-cell-cancer-interactions
#6
Xiaoshan Yue, Trung Dung Nguyen, Victoria Zellmer, Siyuan Zhang, Pinar Zorlutuna
Tumor properties such as growth and metastasis are dramatically dependent on the tumor microenvironment (TME). However, the diversity of the TME including the stiffness and the composition of the extracellular matrix (ECM), as well as the involvement of stromal cells, makes it extremely difficult to establish proper in vitro models for studying tumor growth and metastasis. In this research, we fabricated a stromal cell-laden microwell array system with tunable stiffness ranging from 200 Pa up to 3 kPa, which covers the stiffness range of normal and cancerous mammary tissues, to study the effect of ECM stiffness on stromal-cancer interaction...
April 3, 2018: Biomaterials
https://www.readbyqxmd.com/read/29651473/microfluidic-lung-airway-on-a-chip-with-arrayable-suspended-gels-for-studying-epithelial-and-smooth-muscle-cell-interactions
#7
Mouhita Humayun, Chung-Wai Chow, Edmond W K Young
Chronic lung diseases (CLDs) are regulated by complex interactions between many different cell types residing in lung airway tissues. Specifically, interactions between airway epithelial cells (ECs) and airway smooth muscle cells (SMCs) have been shown in part to play major roles in the pathogenesis of CLDs, but the underlying molecular mechanisms are not well understood. To advance our understanding of lung pathophysiology and accelerate drug development processes, new innovative in vitro tissue models are needed that can reconstitute the complex in vivo microenvironment of human lung tissues...
April 13, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29651265/an-initial-cross-cultural-comparison-of-adult-playfulness-in-mainland-china-and-german-speaking-countries
#8
Dandan Pang, René T Proyer
Compared with playfulness in infants and children, playfulness in adults is relatively under-studied. Although there is no empirical research comparing differences in adult playfulness across cultures, one might expect variations between Western and Eastern societies such as China. While playfulness is typically seen as a positive trait in Western culture, there are hints in Chinese culture that being playful has negative connotations (e.g., associations with laziness and seeing play as the opposite of work)...
2018: Frontiers in Psychology
https://www.readbyqxmd.com/read/29651182/first-report-of-a-new-isolate-of-metarhizium-rileyi-from-maize-fields-of-quivic%C3%A3-n-cuba
#9
Sandra Pérez Álvarez, Amaury Méndez Guerrero, Bernardo Nayar Débora Duarte, Marco Antonio Magallanes Tapia, Jesús Alicia Chávez Medina, Yoannis Domínguez Rodríguez
Metarhizium rileyi (Farlow) Samson is an important entomopathogenic fungus of more than 30 species of Lepidoptera larvae. The aim of this research was to characterize isolate of M. rileyi from Quivicán, Cuba on the basis of morphological and molecular approaches. The fungus was isolated from samples of S . frugiperda larvae collected from maize fields of Quivicán municipality, Mayabeque province, Cuba, and it was cultured on PDA + Ampicillin solid media for morphological characterization. The DNA was isolated using CTAB method and internal transcribed spacer (ITS1, ITS4) were used as the primers for the amplification...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651179/incubation-of-aquilaria-subintegra-with-microbial-culture-supernatants-enhances-production-of-volatile-compounds-and-improves-quality-of-agarwood-oil
#10
Sakon Monggoot, Chadin Kulsing, Yong Foo Wong, Patcharee Pripdeevech
Incubation with microbial culture supernatants improved essential oil yield from Aquilaria subintegra woodchips. The harvested woodchips were incubated with de man, rogosa and sharpe (MRS) agar, yeast mold (YM) agar medium and six different microbial culture supernatants obtained from Lactobacillus bulgaricus , L. acidophilus , Streptococcus thermophilus , Lactococcus lactis , Saccharomyces carlsbergensis and S. cerevisiae prior to hydrodistillation. Incubation with lactic acid bacteria supernatants provided higher yield of agarwood oil (0...
June 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29651134/endothelial-cell-phenotypes-are-maintained-during-angiogenesis-in-cultured-microvascular-networks
#11
Jessica M Motherwell, Christopher R Anderson, Walter L Murfee
A challenge in tissue engineering biomimetic models for studying angiogenesis is building the physiological complexity of real microvascular networks. Our laboratory recently introduced the rat mesentery culture model as an ex vivo experimental platform for investigating multicellular dynamics involved in angiogenesis within intact microvascular networks. The objective of this study was to compare endothelial cell phenotypes along capillary sprouts in cultured ex vivo rat mesentery microvascular networks to in vivo endothelial cell phenotypes...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29645351/a-proteomics-approach-to-identify-candidate-proteins-secreted-by-m%C3%A3-ller-glia-that-protect-ganglion-cells-in-the-retina
#12
Noelia Ruzafa, Xandra Pereiro, Marlen F Lepper, Stefanie M Hauck, Elena Vecino
The retinal Müller glial cells, like other types of glial cells, can enhance the survival and activity of neurons, especially of retinal ganglion cells (RGCs), which are the neurons affected in diseases such as glaucoma, diabetes and retinal ischemia. It has been demonstrated that Müller glia release neurotrophic factors that support RGC survival, yet many of these factors remain to be elucidated. To define these neurotrophic factors, we adopted a quantitative proteomic approach aiming at identifying neuroprotective proteins...
April 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29644877/protective-effects-of-folic-acid-on-dna-damage-and-dna-methylation-levels-induced-by-n-methyl-n-nitro-n-nitrosoguanidine-in-kazakh-esophageal-epithelial-cells
#13
Y Chen, H Feng, D Chen, K Abuduwaili, X Li, H Zhang
The protective effects of folic acid on DNA damage and DNA methylation induced by N-methyl- N'-nitro- N-nitrosoguanidine (MNNG) in Kazakh esophageal epithelial cells were investigated using a 3 × 3 factorial design trial. The cells were cultured in vitro and exposed to media containing different concentrations of folic acid and MNNG, after which growth indices were detected. DNA damage levels were measured using comet assays, and genome-wide DNA methylation levels (MLs) were measured using high-performance liquid chromatography...
January 1, 2018: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29644379/cyanotrophic-and-arsenic-oxidizing-activities-of-pseudomonas-mendocina-p6115-isolated-from-mine-tailings-containing-high-cyanide-concentration
#14
Alejandra Miranda-Carrazco, Juan M Vigueras-Cortés, Lourdes Villa-Tanaca, César Hernández-Rodríguez
Mine tailings and wastewater generate man-made environments with several selective pressures, including the presence of heavy metals, arsenic and high cyanide concentrations, but severe nutritional limitations. Some oligotrophic and pioneer bacteria can colonise and grow in mine wastes containing a low concentration of organic matter and combined nitrogen sources. In this study, Pseudomonas mendocina P6115 was isolated from mine tailings in Durango, Mexico, and identified through a phylogenetic approach of 16S rRNA, gyrB, rpoB, and rpoD genes...
April 11, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/29627547/implementation-of-uv-based-advanced-oxidation-processes-in-algal-medium-recycling
#15
Wenxuan Wang, Jun Sha, Zhiying Lu, Senlin Shao, Peizhe Sun, Qiang Hu, Xuezhi Zhang
Algae show great potential as sustainable feedstock for numerous bioproducts. However, large volume of water consumption during algal biomass production makes that the culture media recycling is a necessity due to economic and environmental concern. To avoid the negative effect of enriched organic matters in the harvested culture media, pre-treatment prior to medium replenishment and reuse is required. In this study, degradation of algenitic organic matters (AOM) in the culture media by UV-based photolysis processes (i...
April 5, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29627251/a-selective-medium-for-the-enumeration-and-differentiation-of-lactobacillus-delbrueckii-ssp-bulgaricus
#16
Nwadiuto O Nwamaioha, Salam A Ibrahim
Modified reinforced clostridial medium (mRCM) was developed and evaluated for the differential enumeration of Lactobacillus delbrueckii ssp. bulgaricus. Lactobacillus bulgaricus, an important species of lactic acid bacteria with health benefits, is used in the production of yogurt and other fermented foods. Our results showed that supplementing reinforced clostridial medium with 0.025% CaCl2 , 0.01% uracil, and 0.2% Tween 80 (mRCM) significantly enhanced the growth rate of L. bulgaricus RR and ATCC 11842 strains as measured by the optical densities of these strains after 12 h of incubation at 42°C...
April 4, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29627157/tgfb1-modulates-in-vitro-secretory-activity-and-viability-of-equine-luteal-cells
#17
António Galvão, Karolina Wolodko, Maria Rosa Rebordão, Dariusz Skarzynski, Graça Ferreira-Dias
In the present report we describe the involvement of transforming growth factor B1 (TGF) in functional regression and structural luteolysis in the mare. Firstly, TGF and its receptors activin-like kinase (ALK) 5 and TGF receptor 2 were identified in corpus luteum (CL) steroidogenic, endothelial and fibroblast-like cells. Also, TGF and ALK5 protein expression were shown to be increased in Mid-, and Late-CL (p < 0.05). Subsequently, using an in vitro model with Mid-CL cells, we studied the role of TGF on secretory activity and cell viability...
April 4, 2018: Cytokine
https://www.readbyqxmd.com/read/29626731/microbial-composition-of-spoiled-industrial-scale-sichuan-paocai-and-characteristics-of-the-microorganisms-responsible-for-paocai-spoilage
#18
Feng Zhang, Yao Tang, Yao Ren, Kai Yao, Qiang He, Yuping Wan, Yuanlong Chi
The microorganisms of spoiled industrial-scale Sichuan paocai (ISSP) were isolated using six types of media, and 16S rRNA and 26S rRNA gene sequence analyses were used to identify the isolates. Meanwhile, the microbial composition was investigated using a culture-independent method through 16S rRNA and ITS sequencing on an Illumina MiSeq platform. The results obtained by these two methods were compared. Furthermore, characteristics of the isolated microorganisms responsible for ISSP spoilage were studied. Sixty-two strains belonging to twenty-three species, including three ammonia-producing genera, two gas-producing genera, two pectinase-producing genera, two cellulase-producing genera, three film-producing genera and one slime-producing genus, were isolated...
April 3, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29626470/exosomal-mir-675-from-metastatic-osteosarcoma-promotes-cell-migration-and-invasion-by-targeting-caln1
#19
Liangzhi Gong, Qiyuan Bao, Chuanzhen Hu, Jun Wang, Qi Zhou, Li Wei, Lei Tong, Weibin Zhang, Yuhui Shen
Exosomal microRNAs(miRNAs) transfer from tumor to stromal cells is reportedly associated with cancer progression and metastasis in various epithelial cancers. However, the role of exosomal miRNA in the metastasis of osteosarcoma(OS) -the most common bone malignancy-still largely remains unknown. In this study, we purified exosomes with a median size close to 100 nm from cell culture media as well as patient serum, and proved that exosomes derived from the metastatic, but not the non-metastatic OS cells increase the migration and invasion of non-malignant fibroblast cells (hFOB1...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29626464/impact-of-l-carnitine-on-lipid-content-and-post-thaw-survivability-of-buffalo-embryos-produced-in-vitro
#20
Megha Verma, Sriti Pandey, Irfan A Bhat, B Mukesh, J Anand, Vikash Chandra, G Taru Sharma
The aim of the present study was to see the impact of L-Carnitine (LC) on lipid biosynthesis and metabolism of buffalo embryos, and post thawed blastocyst survivability. In vitro fertilized (IVF) embryos were derived from slaughterhouse derived COCs and cultured in different doses of LC i.e. 0, 1 mM, 1.5 mM, 2 mM starting at 48 h post IVF. Blastocyst rate was significantly (p < 0.05) higher in 1.5 mM group than control and 1.0 mM group. Lipid content was measured indirectly by fluorescent intensity of lipid droplets after Nile red staining, and it was lower (p < 0...
April 4, 2018: Cryobiology
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