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https://www.readbyqxmd.com/read/28530327/-clinical-aspects-of-intrauterine-insemination
#1
REVIEW
Eliezer Girsh, Semion Meltzer, Buzhena Saar-Ryss, Gad Liberty, Jaakov Rabinson, Shevach Friedler
Intrauterine insemination (IUI) is an intervention performed frequently in the treatment of couples suffering from subfertility and women treated using donor sperm. The factors predicting its success include female parameters such as age and ovarian function and male parameters such as sperm quality. However, many details regarding its clinical application such as the methodology of sperm preparation, timing of IUI, the number of inseminations in the same cycle, the recommended number of cycles to be performed and the minimal threshold values of the sperm sample profile required are controversial...
August 2016: Harefuah
https://www.readbyqxmd.com/read/28509550/sugars-require-rigid-multivalent-displays-for-activation-of-mouse-sperm-acrosomal-exocytosis
#2
He Huang, Maria T Rodolis, Surita Rani Bhatia, Nicole S Sampson
As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose or GlcNAc are effective synthetic inducers of mouse sperm acrosomal exocytosis (AE). Each carbohydrate is proposed to have a distinct binding site on the sperm cell surface. To determine the role of the scaffold structure on efficiency of AE induction, different polymer backbones were employed to display the different activating sugar residues...
May 16, 2017: Biochemistry
https://www.readbyqxmd.com/read/28506466/impaired-steroidogenesis-in-the-testis-of-leptin-deficient-mice-ob-ob
#3
Fabiane Ferreira Martins, Marcia Barbosa Aguila, Carlos Alberto Mandarim-de-Lacerda
The obesity and its comorbidities, including resistance to leptin, impacts the reproductive function. Testes express leptin receptors in the germ cells and Leydig cells. Then, leptin-deficient animals are obese and infertile. We aimed to evaluate the structure and steroidogenic pathway of the testis of deficient leptin mice. Three months old male C57BL/6 mice (wild-type, WT) and deficient leptin (ob/ob) mice had their testes dissected and prepared for analyses. Compared to the WT group, the ob/ob group showed a greater body mass with smaller testes, and alterations in the germinative epithelium: fewer spermatogonia, spermatocytes, and spermatids...
May 13, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28497913/role-of-antioxidants-in-assisted-reproductive-techniques
#4
REVIEW
Ashok Agarwal, Ahmad Majzoub
Oxidative stress (OS) has been recognized as a significant cause of suboptimal assisted reproductive outcome. Many of the sperm preparation and manipulation procedures that are necessary in the in vitro environment can result in excessive production of reactive oxygen species (ROS) thereby exposing the gametes and growing embryos to significant oxidative damage. Antioxidants have long been utilized in the management of male subfertility as they can counterbalance the elevated levels of ROS inducing a high state of OS...
April 30, 2017: World Journal of Men's Health
https://www.readbyqxmd.com/read/28479754/effect-of-body-mass-index-on-the-outcome-of-in-vitro-fertilization-intracytoplasmic-sperm-injection-in-women
#5
Manish Banker, Dipesh Sorathiya, Sandeep Shah
BACKGROUND: Obesity, a known epidemic, is a leading cause of various reproductive disorders. Association of body mass index (BMI) with pregnancy outcomes, either ovarian or endometrial, is controversial and least elucidated. AIM: This study aimed to analyze the effect of BMI on in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) outcome in women using self-oocytes, embryos prepared from donor oocytes (DE), or vitrified/frozen embryos (VE) obtained from both the SE and DE groups...
January 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28470793/protective-effect-of-propolfenol-%C3%A2-on-induced-oxidative-stress-in-human-spermatozoa
#6
M Biagi, G Collodel, M Corsini, N A Pascarelli, E Moretti
The propolis extract was shown to possess the capacity to protect sperm membrane from the deleterious action of oxidative attack. Oxidative stress can induce propagation of a lipid peroxidation (LPO) chain reaction because spermatozoa contain high concentration of unsaturated fatty acids. This study aimed at evaluating in vitro the possible toxicity and/or the antioxidant properties of Propolfenol(®) in ejaculated human spermatozoa. A colorimetric assay determined the total flavonoid content by spectrophotometry and a high-performance liquid chromatography-diode array detection analysis the quantity of galangin, pinocembrin and caffeic acid phenylethilic ester (CAPE)...
May 3, 2017: Andrologia
https://www.readbyqxmd.com/read/28466479/selection-of-viable-human-spermatozoa-with-low-levels-of-dna-fragmentation-from-an-immotile-population-using-density-gradient-centrifugation-and-magnetic-activated-cell-sorting
#7
H Zhang, X Xuan, S Yang, X Li, C Xu, X Gao
We aimed to determine whether density gradient centrifugation and magnetic-activated cell sorting (DGC-MACS) could select viable spermatozoa, with lower levels of DNA fragmentation, from an immotile population. Analysis involved sixteen patients, each with a sperm count ≥10(7) /mL. All samples were immotile despite exhibiting a live population >40%. Spermatozoa were prepared using DGC-MACS and selected spermatozoa evaluated for membrane and DNA integrity using the hypo-osmotic swelling (HOS) test, vital staining and the TUNEL test...
May 3, 2017: Andrologia
https://www.readbyqxmd.com/read/28461086/storage-temperature-and-sucrose-concentrations-affect-ram-sperm-quality-after-vitrification
#8
A Arando, A Gonzalez, J V Delgado, F A Arrebola, C C Perez-Marín
The aim of this study was to analyse the characteristics of ram spermatozoa subjected to varying concentrations of sucrose, and the influence of storage temperature (22°C or 5°C) prior to vitrification. Ejaculated semen was diluted in TCFEY (tris-citric acid-fructose 20% egg yolk), and two aliquots were prepared at a final concentration of 100×10(6)spz/ml, one maintained at room temperature (22°C) and the other at 5°C. In the first experiment, the toxicity of sucrose diluents on the sperm was analysed; sperm samples at different temperatures were diluted (1:2) in TCF-BSA 2% (control) or in the same extender supplemented with various sucrose concentrations (0...
April 25, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28444774/protective-effect-of-l-carnitine-and-l-arginine-against-busulfan-induced-oligospermia-in-adult-rat
#9
A M Abd-Elrazek, O A H Ahmed-Farid
Busulfan is an anticancer drug caused variety of adverse effects for patients with cancer. But it could cause damage to the male reproductive system as one of its adverse effects. This study aimed to investigate the protective effect of L-carnitine and L-arginine on semen quality, oxidative stress parameters and testes cell energy after busulfan treatment. Adult male rats were divided into four groups: control (Con), busulfan (Bus), busulfan plus L-arginine (Bus + L-arg) and busulfan plus L-carnitine (Bus + L-car)...
April 25, 2017: Andrologia
https://www.readbyqxmd.com/read/28440264/use-of-testicular-sperm-for-intracytoplasmic-sperm-injection-in-men-with-high-sperm-dna-fragmentation-a-swot-analysis
#10
Sandro C Esteves, Matheus Roque, Nicolás Garrido
Spermatozoa retrieved from the testis of men with high levels of sperm DNA fragmentation (SDF) in the neat semen tend to have better DNA quality. Given the negative impact of SDF on the outcomes of Assisted Reproductive Technology (ART), an increased interest has emerged about the use of testicular sperm for intracytoplasmic sperm injection (Testi-ICSI). In this article, we used a SWOT (strengths, weaknesses, opportunities, and threats) analysis to summarize the advantages and drawbacks of this intervention...
April 18, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/28385674/human-derived-fusogenic-peptides-for-the-intracellular-delivery-of-proteins
#11
Kei Sudo, Keisuke Niikura, Kouta Iwaki, Shunshi Kohyama, Kei Fujiwara, Nobuhide Doi
The cytosolic delivery of therapeutic proteins (e.g., antibodies or enzymes) by cell-penetrating peptides (CPPs), such as a human immunodeficiency virus-derived TAT peptide, is facilitated by fusogenic peptides (FPs). For instance, we recently demonstrated that an FP, B18, which is derived from a sea urchin gamete fusion protein, promotes endosomal escape of an enhanced green fluorescent protein (eGFP)-TAT fusion protein directly conjugated to it. However, the potential clinical use of FPs raises concerns because all conventional FPs are non-human-derived...
April 4, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28376139/cryopreservation-of-nili-ravi-buffalo-bull-sperm-in-cryodiluent-supplemented-with-lolium-perenne-protein-preparations
#12
S Qadeer, M A Khan, M S Ansari, B A Rakha, R Ejaz, A U Husna, A Azam, N Ullah, V K Walker, S Akhter
BACKGROUND: Semen from the Nili-Ravi buffalo bull, Bubalus bubalis, shows poor survival after freeze storage compared to bovine (Bos taurus and Bos indicus) semen. Freeze-susceptibility distinctions in these two genera have been attributed to differences in sperm membranes. MATERIALS AND METHODS: We measured the impact of protein preparations derived from a frost-resistant perennial grass, Lolium perenne, with ice recrystallization inhibition activity on the low temperature storage of B...
January 2017: Cryo Letters
https://www.readbyqxmd.com/read/28356499/relationship-of-dna-integrity-to-hrg-c633t-snp-and-art-outcome-in-infertile-couples
#13
Arumugam Kumaresan, Anders Johannisson, Sarah Nordqvist, Karin Kårehed, Helena Åkerud, Karin Lindgren, Jane Morrell
The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome or HRG C633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility (n=118) undergoing IVF or ICSI. Sperm DNA integrity was analyzed by flow cytometry using three fluorescent probes (Acridine Orange, Monobromobimane and Chromomycin A3). Principal component analysis was used to identify the parameters that most influenced fertility...
March 29, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28343757/clinical-utility-of-newly-developed-highly-purified-human-menopausal-gonadotrophins-a-randomized-controlled-trial
#14
Hwa Seon Koo, Hwang Kwon, Doo Seok Choi, Song Han, Jung Youn Seo, Kwang Moon Yang
The aim of this study was to evaluate the safety and efficacy of IVF-M HP, a newly developed highly purified human menopausal gonadotrophin preparation, for ovarian stimulation in women with infertility undergoing IVF, intracytoplasmic sperm injection (IVF-ICSI) and embryo transfer using a GnRH antagonist protocol. This was a multicentre, randomized, active-controlled, parallel design, open-label, non-inferiority clinical study. Of the 112 patients randomized for treatment using the GnRH antagonist protocol, 111 were treated...
May 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28322098/effect-of-sperm-dna-fragmentation-on-clinical-outcomes-for-chinese-couples-undergoing-in%C3%A2-vitro-fertilization-or-intracytoplasmic-sperm-injection
#15
Lin-Tao Xue, Rui-Xue Wang, Bing He, Wei-Ying Mo, Li Huang, Shi-Kai Wang, Xian-Bao Mao, Jun-Ping Cheng, Yue-Yue Huang, RuiZhi Liu
Objective To investigate the effect of sperm DNA fragmentation on the fertilization rate, embryo development and pregnancy outcome of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a cohort of Chinese couples. Methods Infertile couples that had undergone assisted reproductive technology at our centre between January 2011 and December 2013 were included in this retrospective study. Fractions of prepared sperm samples were evaluated for sperm DNA fragmentation on the day of oocyte recovery...
December 2016: Journal of International Medical Research
https://www.readbyqxmd.com/read/28306342/nmr-based-plasma-metabolomic-discrimination-for-male-fertility-assessment-of-rats-treated-with-eurycoma-longifolia-extracts
#16
Forough Ebrahimi, Baharudin Ibrahim, Chin-Hoe Teh, Vikneswaran Murugaiyah, Kit-Lam Chan
Male infertility is one of the leading causes of infertility which affects many couples worldwide. Semen analysis is a routine examination of male fertility status which is usually performed on semen samples obtained through masturbation that may be inconvenient to patients. Eurycoma longifolia (Tongkat Ali, TA), native to Malaysia, has been traditionally used as a remedy to boost male fertility. In our recent studies in rats, upon the administration of high-quassinoid content extracts of TA including TA water (TAW), quassinoid-rich TA (TAQR) extracts, and a low-quassinoid content extract including quassinoid-poor TA (TAQP) extract, sperm count (SC) increased in TAW- and TAQR-treated rats when compared to the TAQP-treated and control groups...
June 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28302936/cryotolerance-of-porcine-blastocysts-is-improved-by-treating-in-vitro-matured-oocytes-with-l-carnitine-prior-to-fertilization
#17
Jenna L Lowe, Louise K Bartolac, Roslyn Bathgate, Christopher G Grupen
Sufficient generation of adenosine triphosphate (ATP) by oocytes is critical for fertilization and embryo development. The objective of this study was to determine the effects of supplementing media with L-carnitine, a co-factor required for the metabolism of fatty acids, during the peri-fertilization period on embryo development and energy generation. Firstly, in vitro fertilization (IVM) porcine oocytes were co-incubated with sperm in IVF medium supplemented with 0‒24 mM L-carnitine. The blastocyst formation rate of the control group was greater than those of the L-carnitine groups (P < 0...
March 16, 2017: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/28296340/lactadherin-is-a-candidate-oviduct-lewis-x-trisaccharide-receptor-on-porcine-spermatozoa
#18
E Silva, D Frost, L Li, N Bovin, D J Miller
A recent study has demonstrated that porcine spermatozoa recognize with high affinity carbohydrate structures containing Lewis X motifs. Sperm adhesion to Lewis X is proposed to mediate sperm binding to the oviduct epithelium to form a reservoir. The objective of this study was to identify Lewis X-binding proteins from porcine spermatozoa as candidate receptors for oviduct glycans. To identify low-abundance proteins typically masked by proteins originating from seminal fluid, Lewis X candidate receptors were enriched from cauda epididymal boar spermatozoa...
May 2017: Andrology
https://www.readbyqxmd.com/read/28295487/sperm-dna-fragmentation-as-a-result-of-ultra-endurance-exercise-training-in-male-athletes
#19
D Vaamonde, C Algar-Santacruz, A Abbasi, J M García-Manso
Intensive sports practice seems to exert negative effects on semen parameters; in order to assess these effects, the objective of this study was to assess semen, including DNA fragmentation, and hormone parameters in elite triathletes. Twelve high-level triathletes preparing for a National Triathlon Championship participated in the study. The qualitative sperm parameters analysed were volume, sperm count, motility, morphology and DNA fragmentation; when needed, additional testing was performed. Assessed hormones were testosterone (T), cortisol (C) and testosterone-cortisol ratio (T/C)...
March 15, 2017: Andrologia
https://www.readbyqxmd.com/read/28294638/potential-targetability-of-multi-walled-carbon-nanotube-loaded-with-silver-nanoparticles-photosynthesized-from-ocimum-tenuiflorum-tulsi-extract-in-fertility-diagnosis
#20
Pradeep K Jha, Rakhi K Jha, Dilip Rout, Sathishkumar Gnanasekar, Suresh Vs Rana, Maidul Hossain
Nanocarrier mediated targeted delivery and biosensing in reproductive health care is a major exploratory domain. This work demonstrates the loading of silver nanoparticle (AgNP) inside the multiwalled Carbon nanotube(MWCNT) and their targetability to the intracellular part of the sperm cell for its further application in biosensing based infertility diagnosis. Ocimum tenuiflorum (tulsi extract) mediated photosynthesized AgNP exhibited spherical shape, 5-40nm size and surface plasmonic resonance at 430 nm...
March 15, 2017: Journal of Drug Targeting
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