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https://www.readbyqxmd.com/read/27908215/in-vitro-effect-of-myo-inositol-on-sperm-motility-in-normal-and-oligoasthenospermia-patients-undergoing-in-vitro-fertilization
#1
P G Artini, E Casarosa, E Carletti, P Monteleone, A Di Noia, O M Di Berardino
It is a known fact that abnormal seminal liquid specimens contain abnormal amounts of oxygen free radicals and reactive oxygen species (ROS), and that the use of antioxidant molecules both in vivo and in vitro leads to improvement of semen quality in terms of motility, reduction in DNA damage, with obvious consequences on the fertilization potential. Myo-inositol has been observed to have anti-oxidant properties and be present in much greater concentrations specifically in seminal liquid than in the blood. Moreover, there seems to be a direct relationship between myo-inositol and mitochondrial membrane potential (MMP) and sperm motility...
December 2, 2016: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27883175/heads-you-win-tails-you-lose-filtration-processing-for-the-microscopical-examination-of-sperm-heads
#2
I A Brewis, M A Watkinson, C J VON Ruhland
The sperm head plays a key role in many fertilisation events and determining the precise location of molecules within the head region is important in mechanistically dissecting the fertilisation process. Such molecules may be present in low copy number and many sperm head profiles must be examined to localise them to particular subcellular structures with confidence. Filtration has traditionally been used for the purpose of concentrating biological material, such as free-living cells, spores, and subcellular fractions, and little attempt has been made to extend the procedure to encompass the entire processing schedule, mainly due to the incompatibility of intermediate dehydrating solvents with membrane filters...
November 24, 2016: Journal of Microscopy
https://www.readbyqxmd.com/read/27864361/causes-of-male-infertility-a-9-year-prospective-monocentre-study-on-1737-patients-with-reduced-total-sperm-counts
#3
M Punab, O Poolamets, P Paju, V Vihljajev, K Pomm, R Ladva, P Korrovits, M Laan
STUDY QUESTION: What are the primary causes of severe male factor infertility? SUMMARY ANSWER: Although 40% of all patients showed primary causes of infertility, which could be subdivided into three groups based on the severity of their effect, ~75% of oligozoospermia cases remained idiopathic. WHAT IS KNOWN ALREADY: There are few large-scale epidemiological studies analyzing the causes of male factor infertility. STUDY DESIGN, SIZE, DURATION: A prospective clinical-epidemiological study was conducted at the Andrology Centre, Tartu University Hospital between 2005 and 2013, recruiting male partners of couples failing to conceive a child for over ≥12 months...
November 17, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27852632/predicting-the-chances-of-a-live-birth-after-one-or-more-complete-cycles-of-in-vitro-fertilisation-population-based-study-of-linked-cycle-data-from-113%C3%A2-873-women
#4
David J McLernon, Ewout W Steyerberg, Egbert R Te Velde, Amanda J Lee, Siladitya Bhattacharya
OBJECTIVE:  To develop a prediction model to estimate the chances of a live birth over multiple complete cycles of in vitro fertilisation (IVF) based on a couple's specific characteristics and treatment information. DESIGN:  Population based cohort study. SETTING:  All licensed IVF clinics in the UK. National data from the Human Fertilisation and Embryology Authority register. PARTICIPANTS:  All 253 417 women who started IVF (including intracytoplasmic sperm injection) treatment in the UK from 1999 to 2008 using their own eggs and partner's sperm...
November 16, 2016: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/27818535/amine-reactive-dyes-an-alternative-to-estimate-membrane-integrity-in-fish-sperm-cells
#5
Leticia Torres, Terrence R Tiersch
Fluorescent dyes that binds irreversibly to cellular amines, come in several available emission spectra, and do not poses health concerns were used to evaluate membrane integrity in fish sperm cells. The objectives of the present study were to determine: (1) a working dye concentration for fish sperm samples, and (2) if the traditional propidium iodide/SYBR-14 staining combination was comparable with the amine reactive dye (ARD) methods at identifying cell populations with intact and compromised membranes after sperm activation, refrigerated storage, and exposure to cryoprotectant and surfactant...
October 1, 2016: Aquaculture
https://www.readbyqxmd.com/read/27805545/effect-of-sperm-preparation-on-development-of-bovine-blastocyst-in-vitro
#6
Maria Celina Abraham, Anders Johannisson, Jane M Morrell
Sperm preparation is an important step in the in vitro production of embryos. Centrifugation through colloids has been used to select normal sperm for assisted reproduction in several species. Animal models can sometimes be used as a preliminary step to investigate sperm preparation methods that are potentially of use for human fertility treatments. In this study bovine semen was prepared using three variants of the single-layer centrifugation sperm selection technique (Small, Mini, Mini-EP) with Bovicoll (Androcoll-B)...
December 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27803588/ficoll-400-density-gradient-method-as-an-effective-sperm-preparation-technique-for-assisted-reproductive-techniques
#7
Hyacinth N Highland, A Sharma Rishika, S Shaikh Almira, P Bansal Kanthi
BACKGROUND: Infertility being a burning issue, the male itself contributes about 40% as a cause, as evident by statistical data. However, Assisted Reproductive Technology (ART) has emerged as a powerful tool in the management of infertility. Sperm preparation techniques govern the selection procedure to separate functional spermatozoa which can then be used in IUI, IVF, and ART and for cryopreservation. AIM: The present study was aimed at evaluation of sperm preparation techniques for reliability, performance and to determine the most effective, feasible and economical technique...
July 2016: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/27773484/mechanisms-of-aneuploidy-in-human-eggs
#8
Alexandre Webster, Melina Schuh
Eggs and sperm develop through a specialized cell division called meiosis. During meiosis, the number of chromosomes is reduced by two sequential divisions in preparation for fertilization. In human female meiosis, chromosomes frequently segregate incorrectly, resulting in eggs with an abnormal number of chromosomes. When fertilized, these eggs give rise to aneuploid embryos that usually fail to develop. As women become older, errors in meiosis occur more frequently, resulting in increased risks of infertility, miscarriage, and congenital syndromes, such as Down's syndrome...
September 27, 2016: Trends in Cell Biology
https://www.readbyqxmd.com/read/27758006/mitigation-of-sperm-tail-abnormalities-using-demembranation-approach-in-the-clouded-leopard-neofelis-nebulosa
#9
W Tipkantha, P Thuwanut, B Siriaroonrat, P Comizzoli, K Chatdarong
Clouded leopards (Neofelis nebulosa) produced high proportion of abnormal spermatozoa (mainly tail defects) that can limit sperm movement and conception. The study aimed to better identify the origin of those defects using a demembranation approach. Ejaculates (1-2 ejaculations/male; n = 9) were allocated to simple washing (control; resulting in 11.7% ± 1.9% coiled tails) and processed through colloid centrifugation to reduce the number of sperm with tail defects (treatment, resulting in 5.9% ± 0.9% coiled tails)...
October 18, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27690295/factors-and-pathways-involved-in-capacitation-how-are-they-regulated
#10
Shi-Kai Jin, Wan-Xi Yang
In mammals, fertilization occurs via a comprehensive progression of events. Freshly ejaculated sperm have yet to acquire progressive motility or fertilization ability. They must first undergo a series of biochemical and physiological changes, collectively known as capacitation. Capacitation is a significant prerequisite to fertilization. During the process of capacitation, changes in membrane properties, intracellular ion concentration and the activities of enzymes, together with other protein modifications, induce multiple signaling events and pathways in defined media in vitro or in the female reproductive tract in vivo...
September 27, 2016: Oncotarget
https://www.readbyqxmd.com/read/27678485/uterosome-like-vesicles-prompt-human-sperm-fertilising-capability
#11
A Franchi, M Cubilla, H A Guidobaldi, A A Bravo, L C Giojalas
STUDY QUESTION: Does the rapid transit through the uterine environment modulate the sperm physiological state? SUMMARY ANSWER: The uterosome-like vesicles (ULV) secreted by endometrial epithelial cells (EEC) in vitro are able to fuse with human spermatozoa, prompting their fertilising capacity. WHAT IS KNOWN ALREADY: Early studies suggest that sperm capacitation begins in the uterus and ends in the oviduct, and that a synergistic effect of both female organs may accelerate this process...
September 27, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27678466/puma-puma-concolor-epididymal-sperm-morphometry
#12
Hernán Cucho, Virgilio Alarcón, César Ordóñez, Enrique Ampuero, Aydee Meza, Carles Soler
The Andean puma (Puma concolor) has not been widely studied, particularly in reference to its semen characteristics. The aim of the present study was to define the morphometry of puma sperm heads and classify their subpopulations by cluster analysis. Samples were recovered postmortem from two epididymides from one animal and prepared for morphological observation after staining with the Hemacolor kit. Morphometric data were obtained from 581 spermatozoa using a CASA-Morph system, rendering 13 morphometric parameters...
November 2016: Asian Journal of Andrology
https://www.readbyqxmd.com/read/27672392/in-vitro-antioxidant-treatment-of-semen-samples-in-assisted-reproductive-technology-effects-of-myo-inositol-on-nemaspermic-parameters
#13
Mariangela Palmieri, Palma Papale, Antonietta Della Ragione, Giuseppa Quaranta, Giovanni Russo, Sabatino Russo
Male infertility and the poor quality of sperm seem to be influenced by oxidative stress. In particular, the reactive oxygen species (ROS) mainly produced by morphologically altered spermatozoa affect sperm motility, morphology, and integrity. The aim of this study was to evaluate the efficacy of Myo-Inositol (Myo-Ins) on a number of parameters such as viscosity and total and progressive motility of spermatozoa, in order to better validate its possible practical application in vitro, in order to improve the capacitation protocols commonly used in Assisted Reproductive Technology (ART)...
2016: International Journal of Endocrinology
https://www.readbyqxmd.com/read/27651727/methods-for-endometrial-preparation-in-frozen-thawed-embryo-transfer-cycles
#14
REVIEW
Ziya Kalem, Müberra Namlı Kalem, Timur Gürgan
Frozen-thawed (FT) embryo transfer is a procedure used for the storage and transfer of excess embryos obtained during in vitro fertilization- intracytoplasmic sperm injection cycles. In recent years, improvements in laboratory conditions and limitations on the number of embryos to be transferred have led to a progressive increase in FT embryo transfer cycles. However, the best solution for endometrial preparation in these cycles is still a matter of debate. In this study, we aimed to review the current methods of endometrial preparation in FT embryo transfer cycles...
2016: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/27651720/factors-affecting-clinical-pregnancy-rates-after-iui-for-the-treatment-of-unexplained-infertility-and-mild-male-subfertility
#15
Melahat Atasever, Müberra Namlı Kalem, Şafak Hatırnaz, Ebru Hatırnaz, Ziya Kalem, Zeynep Kalaylıoğlu
OBJECTIVE: The aim of the present retrospective study was to evaluate intrauterine insemination (IUI) clinical experiences and to define the variables for predicting success. MATERIAL AND METHODS: The present study was an observational trial performed in a private IVF center on subfertile couples who had applied for treatment between 2002 and 2012, in which the data of 503 IUI cases were retrospectively reviewed. Couples who had been diagnosed with unexplained and mild male subfertility were included...
2016: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/27651517/why-doesn-t-conventional-ivf-work-in-the-horse
#16
Bart Leemans, Bart M Gadella, Tom Arjun Edgar Stout, Catharina De Schauwer, Hilde Maria Nelis, Maarten Hoogewijs, Ann Van Soom
In contrast to man and many other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. The apparent inability of stallion spermatozoa to penetrate the zona pellucida in vitro is most likely due to incomplete activation of spermatozoa (capacitation) as a result of inadequate capacitating or fertilizing media. In vivo, the oviduct and its secretions provide a micro-environment that does reliably support and regulate interaction between the gametes. This review focuses on equine sperm-oviduct interaction...
September 20, 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27646677/improving-icsi-a-review-from-the-spermatozoon-perspective
#17
Mara Simopoulou, Laertis Gkoles, Panagiotis Bakas, Polina Giannelou, Theodoros Kalampokas, Konstantinos Pantos, Michael Koutsilieris
: Intracytoplasmic sperm injection (ICSI) is the most frequently applied method for fertilization making the process of identifying the perfect spermatozoon fundamental. Herein we offer a critical and thorough presentation on the techniques reported regarding (i) handling and preparing semen samples, (ii) identifying and 'fishing' spermatozoa, and (iii) improving key factors, such as motility for a successful ICSI practice. These approaches are suggested to make the process easier and more effective especially in atypical and challenging circumstances...
December 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27638141/the-development-of-a-weak-anion-micro-capillary-film-for-protein-chromatography
#18
A J M Kouyoumdjian, R A Lazar, N K H Slater
In this study, the surface of a microporous walled micro-capillary film (MMCF) was modified into a weak anion exchanger by coupling cyanuric chloride and 2-diethylaminoethylamine (DEAE) to the ethylene-vinyl alcohol (EVOH) matrix. Fourier transform infrared spectroscopy (FTIR) measurements of modified and unmodified MMCFs confirmed the addition of a triazine ring and DEAE onto the membrane. Binding of bovine serum albumin (BSA) at pH 7.2 was found to follow a Langmuir isotherm with a maximum equilibrium binding of 12...
October 14, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27637002/white-tea-intake-prevents-prediabetes-induced-metabolic-dysfunctions-in-testis-and-epididymis-preserving-sperm-quality
#19
Tânia R Dias, Marco G Alves, Luís Rato, Susana Casal, Branca M Silva, Pedro F Oliveira
Prediabetes has been associated with alterations in male reproductive tract, especially in testis and epididymis. Moreover, in vitro studies described a promising action of tea (Camellia sinensis L.) against metabolic dysfunctions. Herein, we hypothesized that white tea (WTEA) ingestion by prediabetic animals could ameliorate the metabolic alterations induced by the disease in testicular and epididymal tissues, preserving sperm quality. WTEA infusion was prepared and its phytochemical profile was evaluated by (1)H-NMR...
August 26, 2016: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/27628424/global-analysis-of-differential-gene-expression-related-to-long-term-sperm-storage-in-oviduct-of-chinese-soft-shelled-turtle-pelodiscus-sinensis
#20
Tengfei Liu, Ping Yang, Hong Chen, Yufei Huang, Yi Liu, Yasir Waqas, Nisar Ahmed, Xiaoya Chu, Qiusheng Chen
Important evolutionary and ecological consequences arise from the ability of female turtles to store viable spermatozoa for an extended period. Although previous morphological studies have observed the localization of spermatozoa in Pelodiscus sinensis oviduct, no systematic study on the identification of genes that are involved in long-term sperm storage has been performed. In this study, the oviduct of P. sinensis at different phases (reproductive and hibernation seasons) was prepared for RNA-Seq and gene expression profiling...
2016: Scientific Reports
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