Nicole O McPherson, Mark Nottle, Stephan McIlfatrick, Anmol Saini, Hamish Hamilton, Emily Bowman, Cathryn A Tully, Leanne Pacella-Ince, Deirdre Zander-Fox, Hassan W Bakos
RESEARCH QUESTION: Can the addition of progesterone and neurotensin, molecular agents found in the female reproductive tract, after sperm washing increase the fertilization potential of human spermatozoa? DESIGN: (i) Cohort study of 24 men. Spermatozoa selected by swim-up were incubated in either progesterone or neurotensin (0.1-100 µM) for 1-4 h, and hyperactive motility and binding to hyaluronan (0.1-100 µM) were assessed. The effect of progesterone 10 µM on sperm function was assessed in a blinded manner, including: hyperactive motility, binding to hyaluronan, tyrosine phosphorylation, acrosome reaction and oxidative DNA damage...
October 20, 2023: Reproductive Biomedicine Online