keyword
MENU ▼
Read by QxMD icon Read
search

Sperm preparation

keyword
https://www.readbyqxmd.com/read/28216914/the-quality-of-sperm-preparation-medium-affects-the-motility-viability-and-dna-integrity-of-human-spermatozoa
#1
Fatemeh Anbari, Iman Halvaei, Ali Nabi, Shahin Ghazali, Mohammad Ali Khalili, Lars Johansson
AIM: The goal was to compare the effects of three different sperm preparation media on sperm motility, viability, and DNA integrity of semen samples from normozoospermic men. METHODS: A total of 15 normozoospermic males were included in the study. The semen analysis (SA) was performed in accordance with the WHO guidelines (2010). After SA, each sample was divided into three aliquots, and swim-up was performed with three different sperm preparation media (Sperm Preparation Media, Origio, Denmark; Ham's F10, Biochrome, Berlin, Germany; and VitaSperm™, Innovative Biotech, Iran)...
October 2016: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28189123/impact-of-various-progestins-with-or-without-transdermal-testosterone-on-gonadotropin-levels-for-non-invasive-hormonal-male-contraception-a-randomized-clinical-trial
#2
M Zitzmann, J Rohayem, J Raidt, S Kliesch, N Kumar, R Sitruk-Ware, E Nieschlag
Although several progestins have been tested for hormonal male contraception, the effects of dosage and nature of various progestins on gonadotropin suppression combined with and without additional testosterone has not been performed in a comparative trial. The aim of this study was to evaluate the differential impact of four oral or transdermal progestins on the suppression of gonadotropins in healthy men: oral: cyproterone acetate (CPA), levonorgestrel (LNG), norethisterone acetate (NETA), and transdermal: Nestorone(®) (NES), all in combination with transdermal testosterone (T)...
February 11, 2017: Andrology
https://www.readbyqxmd.com/read/28151076/histological-study-on-the-effect-of-aroclor-1254-on-the-epididymis-of-adult-rats-and-the-role-of-l-name-administration
#3
Nehad F Mazen, Rania A Zidan
INTRODUCTION: Aroclor 1254, a commercial polychlorinated biphenyls (PCBs) mixture, was found to elicit various adverse effects on human health. OBJECTIVES: This study aimed to investigate the structural alterations in the epididymis induced by aroclor 1254, and to assess the possible protective role of L-NAME (NG-Nitro-L arginine methyl ester). MATERIALS AND METHODS: Thirty-five adult male albino rats were divided into three groups: control group (15 rats), equally subdivided into subgroup a; negative control group, subgroup b: received intraperitoneal corn oil (5 ml/kg/day), and subgroup c: received intraperitoneal L-NAME (10 mg/kg/day)...
February 2, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28138845/whole-genome-haplotyping-of-single-sperm-of-daphnia-pulex-crustacea-anomopoda
#4
Sen Xu, Kim Young
Sequencing the entire genome of single sperm cells can provide valuable information of the distribution of meiotic recombination events in eukaryotic genomes. Here, we provide a description of the experimental work flow for isolating single sperm cells from the microcrustacean Daphnia pulex using fluorescence-activated cell sorting. Moreover, we describe the application of a whole-genome amplification technique (i.e., Multiple Annealing and Looping Based Amplification Cycles method) to single sperm of Daphnia to generate enough DNA for library preparation of next-generation sequencing...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28117911/sperm-dimorphism-in-the-triatoma-brasiliensis-species-complex-and-its-applications
#5
A F Baffa, D C P Camara, J R Santos-Mallet, E R Dasilva, J Costa, S P C Freitas
Morphological and structural features of the sperm of the Triatoma brasiliensis Neiva, 1911 (Hemiptera: Reduviidae) species complex were examined in this first study investigating the sperm of Heteroptera and the genus Triatoma. Males were dissected and their seminal vesicles removed. For measurement, seminal vesicles were squashed on glass slides, spread, fixed and observed under a photomicroscope. The images were analysed and measures of sperm were made. Data were analysed using one-way analysis of variance and Tukey's test to detect differentiation among taxa...
January 24, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28110726/applying-data-mining-techniques-for-increasing-implantation-rate-by-selecting-best-sperms-for-intra-cytoplasmic-sperm-injection-treatment
#6
Seyed Abolghasem Mirroshandel, Fatemeh Ghasemian, Sara Monji-Azad
BACKGROUND AND OBJECTIVE: Aspiration of a good-quality sperm during intracytoplasmic sperm injection (ICSI) is one of the main concerns. Understanding the influence of individual sperm morphology on fertilization, embryo quality, and pregnancy probability is one of the most important subjects in male factor infertility. Embryologists need to decide the best sperm for injection in real time during ICSI cycle. Our objective is to predict the quality of zygote, embryo, and implantation outcome before injection of each sperm in an ICSI cycle for male factor infertility with the aim of providing a decision support system on the sperm selection...
December 2016: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28063926/luteinizing-hormone-modulates-intracellular-calcium-protein-tyrosine-phosphorylation-and-motility-during-human-sperm-capacitation
#7
Aideé S López-Torres, María E González-González, Esperanza Mata-Martínez, Fernando Larrea, Claudia L Treviño, Mayel Chirinos
In order to fertilize, spermatozoa must undergo physiological and biochemical changes during their transit along the female reproductive tract before reaching and fusing with the oocyte, process known as capacitation. Sperm modifications associated with capacitation are modulated by their interaction with molecules present in the female reproductive tract. During the woman fertile window, some reproductive hormones reach their maximum concentrations in serum, such as the luteinizing hormone (LH). Since spermatozoa preparing to fertilize may be exposed to LH, the purpose of this work was to study the effects of this hormone on intracellular Ca(2+) concentrations ([Ca(2+)]i), protein tyrosine phosphorylation, sperm motility and acrosome reaction under capacitating conditions...
January 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28056797/comparative-proteomic-analysis-reveals-a-dynamic-pollen-plasma-membrane-protein-map-and-the-membrane-landscape-of-receptor-like-kinases-and-transporters-important-for-pollen-tube-growth-and-interaction-with-pistils-in-rice
#8
Ning Yang, Tai Wang
BACKGROUND: The coordination of pollen tube (PT) growth, guidance and timely growth arrest and rupture mediated by PT-pistil interaction is crucial for the PT to transport sperm cells into ovules for double fertilization. The plasma membrane (PM) represents an important interface for cell-cell interaction, and PM proteins of PTs are pioneers for mediating PT integrity and interaction with pistils. Thus, understanding the mechanisms underlying these events is important for proteomics. RESULTS: Using the efficient aqueous polymer two-phase system and alkali buffer treatment, we prepared high-purity PM from mature and germinated pollen of rice...
January 5, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28050954/comparison-of-two-sperm-processing-techniques-for-low-complexity-assisted-fertilization-sperm-washing-followed-by-swim-up-and-discontinuous-density-gradient-centrifugation
#9
Cássio L Fácio, Lígia F Previato, Ligiane A Machado-Paula, Paulo Cs Matheus, Edilberto Araújo
OBJECTIVE: This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals. METHODS: Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sperm washing followed by swim-up, and 41 (group 2) by discontinuous density gradient centrifugation...
December 1, 2016: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28042543/relationship-between-potential-sperm-factors-involved-in-oocyte-activation-and-sperm-dna-fragmentation-with-intra-cytoplasmic-sperm-injection-clinical-outcomes
#10
Marziyeh Tavalaee, Abbas Kiani-Esfahani, Mohammad Hossein Nasr-Esfahani
OBJECTIVE: The present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath WW domain-binding protein (PAWP), phospholipase Cζ (PLCζ), and truncated form of the kit receptor (TR-KIT)] as candidates of oocyte activation with fertilization rate and early embryonic development. MATERIALS AND METHODS: In this experimental study, semen samples were collected from 35 intra-cytoplasmic sperm injection (ICSI) candidates and analyzed according to World Health Organization criteria (2010)...
2017: Cell Journal
https://www.readbyqxmd.com/read/27995374/nuclear-migration-during-karyogamy-in-rice-zygotes-is-mediated-by-continuous-convergence-of-actin-meshwork-toward-the-egg-nucleus
#11
Yukinosuke Ohnishi, Takashi Okamoto
Fertilization is comprised of two sequential fusion processes; plasmogamy and karyogamy. Karyogamy completes with migration and fusion of the male and female nuclei in the fused cell. In animals, microtubules organized by the centrosome control female/male pronuclei migration. In contrast, the nuclear migration in fused gametes of angiosperms is controlled by actin filaments, but the mechanism that regulates actin filament-dependent nuclear migration is not clear. In this study, we prepared fused rice (Oryza sativa L...
December 19, 2016: Journal of Plant Research
https://www.readbyqxmd.com/read/27958357/semen-preparation-methods-and-sperm-telomere-length-density-gradient-centrifugation-versus-the-swim-up-procedure
#12
Feifei Zhao, Qingling Yang, Senlin Shi, Xiaoyan Luo, Yingpu Sun
Previous studies have shown that both density gradient centrifugation (DGC) and swim up (SU) procedures can select spermatozoa with longer telomeres for assisted reproduction techniques (ART). However, it is unknown which approach is more effective. The aim of the present study was to compare the effects of these two methods on sperm telomere length (STL). A total of 150 normozoospermic subjects were recruited. STL, DNA fragmentation index (DFI), reactive oxygen species (ROS) content and progressive motility of semen samples were detected before and after the procedures of DGC and SU...
December 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27938385/comparison-of-the-effect-of-a-combination-of-eight-micronutrients-versus-a-standard-mono-preparation-on-sperm-parameters
#13
Markus Lipovac, Florian Bodner, Martin Imhof, Peter Chedraui
BACKGROUND: There are reports showing that l-carnitine alone or in combination with other micronutrients improve sperm parameters. However, comparative studies are still lacking. This study was carried out to compare the short term effects of a combination of eight micronutrients including l-carnitine vs. a mono-substance (l-carnitine alone) on sperm parameters. METHODS: This was a prospective, open-labelled, nonrandomized study that included male subjects (20 to 60 years) with at least 1 year of subfertility and at least one pathological semen analysis who received 3 months treatment with a mono-substance (500 mg l-carnitine/twice a day, n = 156) or a combined compound (440 mg l-carnitine + 250 mg l-arginine + 40 mg zinc + 120 mg vitamin E + 80 mg glutathione + 60 μg selenium + 15 mg coenzyme Q10 + 800 μg folic acid/once a day, n = 143) for the same time period...
December 9, 2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27933295/sperm-chromatin-dispersion-test-before-sperm-preparation-is-predictive-of-clinical-pregnancy-in-cases-of-unexplained-infertility-treated-with-intrauterine-insemination-and-induction-with-clomiphene-citrate
#14
Frank W R C Vandekerckhove, Ilse De Croo, Jan Gerris, Etienne Vanden Abbeel, Petra De Sutter
BACKGROUND/AIMS: A large proportion of men with normal sperm results as analyzed using conventional techniques have fragmented DNA in their spermatozoa. We performed a prospective study to examine the incidence of DNA fragmentation in sperm in cases of couples with previously unexplained infertility and treated with intrauterine insemination. We evaluated whether there was any predictive value of DNA fragmentation for pregnancy outcome in such couples. METHODS: The percentage of DNA fragmentation and all classical variables to evaluate sperm before and after sperm treatment were determined...
2016: Frontiers in Medicine
https://www.readbyqxmd.com/read/27925001/dehydration-preparation-of-mouse-sperm-for-vitrification-and-rapid-laser-warming
#15
E Paredes, P Mazur
BACKGROUND: Mice are fundamental models of study due to their ease of breeding, manipulation, and the well-studied genome. There has been extensive research focused on the cryopreservation of mouse germaplasm, as a way to help maintain the different transgenic mouse breeds. The first protocols for mouse sperm were developed in the 90's using slow cooling and a mixture of raffinose and glycerol. Since then, the rate of success reported remains highly variable. OBJECTIVE: The Aim of this work is to study factors that are key for developing vitrification protocols for ultra-rapid laser warming of mouse sperm...
September 2016: Cryo Letters
https://www.readbyqxmd.com/read/27908215/in-vitro-effect-of-myo-inositol-on-sperm-motility-in-normal-and-oligoasthenospermia-patients-undergoing-in-vitro-fertilization
#16
P G Artini, E Casarosa, E Carletti, P Monteleone, A Di Noia, O M Di Berardino
It is a known fact that abnormal seminal liquid specimens contain abnormal amounts of oxygen free radicals and reactive oxygen species (ROS), and that the use of antioxidant molecules both in vivo and in vitro leads to improvement of semen quality in terms of motility, reduction in DNA damage, with obvious consequences on the fertilization potential. Myo-inositol has been observed to have anti-oxidant properties and be present in much greater concentrations specifically in seminal liquid than in the blood. Moreover, there seems to be a direct relationship between myo-inositol and mitochondrial membrane potential (MMP) and sperm motility...
February 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27883175/heads-you-win-tails-you-lose-filtration-processing-for-the-microscopical-examination-of-sperm-heads
#17
I A Brewis, M A Watkinson, C J VON Ruhland
The sperm head plays a key role in many fertilisation events and determining the precise location of molecules within the head region is important in mechanistically dissecting the fertilisation process. Such molecules may be present in low copy number and many sperm head profiles must be examined to localise them to particular subcellular structures with confidence. Filtration has traditionally been used for the purpose of concentrating biological material, such as free-living cells, spores, and subcellular fractions, and little attempt has been made to extend the procedure to encompass the entire processing schedule, mainly due to the incompatibility of intermediate dehydrating solvents with membrane filters...
November 24, 2016: Journal of Microscopy
https://www.readbyqxmd.com/read/27864361/causes-of-male-infertility-a-9-year-prospective-monocentre-study-on-1737-patients-with-reduced-total-sperm-counts
#18
M Punab, O Poolamets, P Paju, V Vihljajev, K Pomm, R Ladva, P Korrovits, M Laan
STUDY QUESTION: What are the primary causes of severe male factor infertility? SUMMARY ANSWER: Although 40% of all patients showed primary causes of infertility, which could be subdivided into three groups based on the severity of their effect, ~75% of oligozoospermia cases remained idiopathic. WHAT IS KNOWN ALREADY: There are few large-scale epidemiological studies analyzing the causes of male factor infertility. STUDY DESIGN, SIZE, DURATION: A prospective clinical-epidemiological study was conducted at the Andrology Centre, Tartu University Hospital between 2005 and 2013, recruiting male partners of couples failing to conceive a child for over ≥12 months...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27852632/predicting-the-chances-of-a-live-birth-after-one-or-more-complete-cycles-of-in-vitro-fertilisation-population-based-study-of-linked-cycle-data-from-113%C3%A2-873-women
#19
David J McLernon, Ewout W Steyerberg, Egbert R Te Velde, Amanda J Lee, Siladitya Bhattacharya
OBJECTIVE:  To develop a prediction model to estimate the chances of a live birth over multiple complete cycles of in vitro fertilisation (IVF) based on a couple's specific characteristics and treatment information. DESIGN:  Population based cohort study. SETTING:  All licensed IVF clinics in the UK. National data from the Human Fertilisation and Embryology Authority register. PARTICIPANTS:  All 253 417 women who started IVF (including intracytoplasmic sperm injection) treatment in the UK from 1999 to 2008 using their own eggs and partner's sperm...
November 16, 2016: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/27818535/amine-reactive-dyes-an-alternative-to-estimate-membrane-integrity-in-fish-sperm-cells
#20
Leticia Torres, Terrence R Tiersch
Fluorescent dyes that binds irreversibly to cellular amines, come in several available emission spectra, and do not poses health concerns were used to evaluate membrane integrity in fish sperm cells. The objectives of the present study were to determine: (1) a working dye concentration for fish sperm samples, and (2) if the traditional propidium iodide/SYBR-14 staining combination was comparable with the amine reactive dye (ARD) methods at identifying cell populations with intact and compromised membranes after sperm activation, refrigerated storage, and exposure to cryoprotectant and surfactant...
October 1, 2016: Aquaculture
keyword
keyword
95740
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"