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Sperm preparation

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https://www.readbyqxmd.com/read/28904906/a-step-by-step-guide-to-office-based-sperm-retrieval-for-obstructive-azoospermia
#1
REVIEW
Robert M Coward, Jesse N Mills
A variety of surgical options exists for sperm retrieval in the setting of obstructive azoospermia (OA). With appropriate preparation, the majority of these techniques can safely be performed in the office with local anesthesia and with or without monitored anesthesia care (MAC). The available techniques include percutaneous options such as percutaneous epididymal sperm aspiration (PESA) and testicular sperm aspiration (TESA), as well as open techniques that include testicular sperm extraction (TESE) and microsurgical epididymal sperm aspiration (MESA)...
August 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28904502/antispermatogenic-mechanism-of-trona-is-associated-with-lipid-peroxidation-but-not-testosterone-suppression
#2
Ayodeji F Ajayi, Roland E Akhigbe
BACKGROUND: About half of the cases of infertility in couples have been attributed to male factor. Despite the claim in folklore medicine that trona (a sesquicarbonate or hydrated carbonate of sodium) causes fetal loss, its effect on male reproductive function has not been investigated. AIM: This study sought to provide scientific evidence on the effect of trona on sperm characteristics, male reproductive hormones and organs, and lipid peroxidation. MATERIALS AND METHODS: Forty male Wistar rats of comparable weights were used for the study...
April 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28878960/eversion-and-withdrawal-of-an-intromittent-organ-before-sexual-maturation-prepares-male-beetles-for-copulation
#3
Yoko Matsumura, Takuya Kubo
Some species of criocerine beetles have a hyper-elongated part of the intromittent organ called a flagellum. In resting position, the flagellum is stored in a specialized internal sac in the intromittent organ. This specialized state of the flagellum and internal sac is indispensable during copulation for flagellar insertion into the female spermathecal duct for sperm transfer. However, the morphogenesis of the flagellum does not generate the active state of the flagellum; rather, the flagellum is generated in an inactive and completely coiled state...
August 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28869110/anandamide-exerts-a-suppressive-effect-on-sperm-binding-to-oviduct-explants-through-cb1-receptors-in-the-water-buffalo-bubalus-bubalis
#4
Vimlesh Kumar, Arumugam Kumaresan, Puneeth Kumar D S, Sreela Lathika, Samiksha Nayak, Kaustubh Kishor Saraf, Pradeep Nag B S, Shivani Chhillar, Tirtha Kumar Datta, Tushar Kumar Mohanty
An endocannabinoid system comprising of Anandamide (AEA) and its receptor has been shown to play a role in sperm acquisition of fertilizing potential and sperm-oviduct interaction. In the present study, we assessed the effect of sperm pre-treatment with AEA or co-incubation of sperm-oviduct explants with AEA in the presence or absence of CB1 receptor antagonist (SR141716A) on sperm-oviduct binding in the water buffalo. Cryopreserved spermatozoa from 3 Murrah buffalo bulls (3 ejaculates from each bull) were utilized for the study...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28868247/vitrification-increased-vacuolization-of-human-spematozoa-application-of-msome-technology
#5
Sara Taherzadeh, Mohammad Ali Khalili, Azam Agha-Rahimi, Fateme Anbari, Shahin Ghazali, Guido Macchiarelli
BACKGROUND: Sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (LN2). The aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by MSOME technology and the fertility potential by zona binding assay (ZBA). METHODS: 20 normo-ejaculates were prepared by swim up technique, and supernatants were divided into two parts of fresh and vitrified groups...
April 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28854719/techniques-used-for-iui-is-it-time-for-a-change
#6
L Lemmens, S Kos, C Beijer, D D M Braat, W L D M Nelen, A M M Wetzels
STUDY QUESTION: Are the guidelines for the technical aspects of IUI (WHO, 2010) still in accordance with the current literature? SUMMARY ANSWER: In general, the laboratory guidelines of the World Health Organization (WHO) are a suitable protocol, although the evidence is not always conclusive and some changes are advisable. WHAT IS KNOWN ALREADY: Lack of standardization of the technical procedures required for IUI might result in inter-laboratory variation in pregnancy rates...
September 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28853925/sex-determination-in-the-mammalian-germline
#7
Cassy Spiller, Peter Koopman, Josephine Bowles
Sexual reproduction crucially depends on the production of sperm in males and oocytes in females. Both types of gamete arise from the same precursor, the germ cells. We review the events that characterize the development of germ cells during fetal life as they commit to, and prepare for, oogenesis or spermatogenesis. In females, fetal germ cells enter meiosis, whereas in males they delay meiosis and instead lose pluripotency, activate an irreversible program of prospermatogonial differentiation, and temporarily cease dividing...
August 30, 2017: Annual Review of Genetics
https://www.readbyqxmd.com/read/28836411/evidence-of-oocyte-polarity-in-bovine-implications-for-intracytoplasmic-sperm-injection-and-somatic-cell-nuclear-transfer
#8
Seyed Morteza Hosseini, Fariba Moulavi, Nima TanhaieVash, Naser Shams-Esfandabadi, Mohammad Hossein Nasr-Esfahani, Abolfazl Shirazi
OBJECTIVES: We recently demonstrated spatial regionalization of maternal transcripts and proteins within unfertilized ovine oocyte. Here, we investigated the likelihood of oocyte polarity for the first time in bovine. MATERIALS AND METHODS: In this experimental study, in vitro matured bovine oocytes were used for manual bisection [into oocyte halve that were near-to (HNS) and far-from (FS) spindle] or trisection [into MII-spindle (S), the spindle-side half (NS), and the distal half unassociated with the spindle (FS)]...
October 2017: Cell Journal
https://www.readbyqxmd.com/read/28830817/evaluation-of-the-aphrodisiac-potential-of-a-chemically-characterized-aqueous-extract-of-tamarindus-indica-pulp
#9
Amita Rai, Snehashis Das, Mallikarjuna Rao Chamallamudi, Krishnadas Nandakumar, Raghavendra Shetty, Meghna Gill, Suhani Sumalatha, Raviraj Devkar, Karthik Gourishetti, Nitesh Kumar
ETHNOPHARMACOLOGICAL RELEVANCE: Tamarindus indica is an ingredient in the traditional aphrodisiac formulations in Africa and India. It is also a widely used food ingredient in other tropical countries. AIM OF THE STUDY: The present study was aimed to evaluate the aphrodisiac potential and reproductive safety profile of aqueous extract of Tamarindus indica in male Wistar rats. MATERIALS AND METHODS: The aqueous extract was prepared by maceration of pulp followed by reduction of volume in rotavapor under heat followed by freeze drying...
August 19, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28815355/egg-donation-of-vitrified-oocytes-bank-produces-similar-pregnancy-rates-by-blastocyst-transfer-when-compared-to-fresh-cycle
#10
Thais S Domingues, Ana Paula Aquino, Bruna Barros, Raquel Mazetto, Mariana Nicolielo, Carolina M Kimati, Talita Devecchi, Tatiana C S Bonetti, Paulo C Serafini, Eduardo L A Motta
PURPOSE: Advances in reproductive techniques, mainly the introduction of oocyte vitrification, have provided the opportunity to conceive from oocyte banks. The aim of this study was to compare the clinical outcomes of fresh and vitrified oocytes in an egg donation program following blastocyst transfer. METHODS: This retrospective observational study included 504 oocyte donation cycles. All donor women were younger than 30 years of age. The recipient cycles were divided into two groups: fresh oocytes (n = 78) or vitrified oocytes (n = 426)...
August 16, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28765033/assessment-of-butorphanol-azaperone-medetomidine-combination-as-anesthesia-for-semen-collection-and-evaluation-of-semen-quality-in-white-tailed-deer-odocoileus-virginianus
#11
S M Kirschner, R Rodenkirch
The aim of this current study was to evaluate the level of anesthesia produced by a combination of butorphanol-azaperone-medetomidine (BAM) for semen collection by electroejaculation on captive white-tailed bucks (Odocoileus virginianus). Ten male white-tailed deer, weighing 68.2-115.9kg, ranging in age from one to four years were randomly selected from housing pens and anesthetized with the BAM drug combination at a dose volume of 2.0mL each. Semen was collected from each animal using a standard cervid electroejaculation protocol while under BAM anesthesia...
July 25, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28752591/comparison-and-evaluation-of-capacitation-and-acrosomal-reaction-in-freeze-thawed-human-ejaculated-spermatozoa-treated-with-l-carnitine-and-pentoxifylline
#12
E Aliabadi, S Jahanshahi, T Talaei-Khozani, M Banaei
Cryopreservation is used to preserve the spermatozoa; however, it leads to a reduction in sperm quality. L-carnitine (LC) influences sperm motility and preserves the sperm membrane and DNA integrity. The objectives of this study were to evaluate the protective effects of LC on the membrane integrity of normal human spermatozoa and compare it with pentoxifylline (PT) during cryopreservation. Thirty normal semen samples, prepared by swim-up procedure, were divided into three aliquots: a control without any treatment and two experimental aliquots that were incubated in PT or LC for 30 min...
July 28, 2017: Andrologia
https://www.readbyqxmd.com/read/28744317/podophyllotoxin-extracted-from-juniperus-sabina-fruit-inhibits-rat-sperm-maturation-and-fertility-by-promoting-epididymal-epithelial-cell-apoptosis
#13
Shuwu Xie, Guoting Li, Lijuan Qu, Ruihua Zhong, Ping Chen, Zhigang Lu, Jieyun Zhou, Xiangjie Guo, Zhao Li, Aying Ma, Yueying Qian, Yan Zhu
This study aimed to investigate the antifertility effect of Juniperus sabina fruit on male rats and its possible mechanism, and hence it might be developed as a potential nonhormonal male contraceptive. Male rats were intragastrically fed for consecutive 8-week and 4-week recovery with the fruit of J. Sabina, and sperm maturation, serum testosterone level, and histopathology were analyzed. Epididymal epithelial cell culture was prepared for detection of podophyllotoxin activities. Furthermore, cell proliferation, transmission electron microscopy, Annexin V/Propidium iodide, TUNEL, RT-PCR, ELISA, and western blotting were examined...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28732188/adapted-methods-for-scanning-electron-microscopy-in-assessment-of-human-sperm-morphology
#14
Petra Nussdorfer, Ines Cilenšek, Branko Zorn, Daniel Petrovič
Infertility is a widespread problem, and in some cases, the routine basic semen analysis is not sufficient to detect the cause of male infertility. The use of the scanning electron microscope (SEM) could provide a detailed insight into spermatozoa morphology, but it requires specific sample preparation techniques. The purpose of this study was to select, adjust, and optimize a method for the preparation of spermatozoa samples prior to SEM analysis, and to establish the protocol required for its use in clinical practice...
July 21, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28731265/application-of-dead-end-knockout-zebrafish-as-recipients-of-germ-cell-transplantation
#15
Qian Li, Wataru Fujii, Kunihiko Naito, Goro Yoshizaki
Germ cell transplantation is a promising technology for the propagation of endangered or valuable fishes. In this technique, sterile male and female recipient fish are injected with donor germ cells so they can produce viable gametes derived from the donor cells. The dead end (dnd) gene is involved in the migration of primordial germ cells; therefore, dnd-knockout zebrafish are expected to be germ-cell-free, making them suitable recipients for germ cell transplantation. dnd mutants were produced by microinjecting 2 nl of 10 ng/μl cRNAs encoding zinc finger nucleases against dnd into the blastodisc of zebrafish embryos before the cell cleavage stage...
July 21, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28709686/toxicologic-evaluations-of-recombinant-liver-targeting-interferon-ifn-csp-genotoxicity-and-tegenicratoity
#16
Wenting Zeng, Chunxu Wu, Jie Wang, Lingjie Cao, Xiaobao Jin, Jiayong Zhu, Xuemei Lu
Interferon alpha as the one of FDA recommended drugs for Hepatitis B virus (HBV) infection has many side effects. Targeting IFNα to the liver may be a strategy to increase its efficacy locally and may increase efficacy of IFNα-based therapy of HBV infection. We have prepared a novel liver-targeting fusion interferon (IFN-CSP) combining IFN α2b with plasmodium region I peptide and have revealed it may be an excellent candidate as a liver-targeting anti-HBV agent. In this study, we investigated the genotoxic and teratogenic effects of IFN-CSP...
July 11, 2017: Regulatory Toxicology and Pharmacology: RTP
https://www.readbyqxmd.com/read/28709083/the-peculiar-extra-acrosomal-structure-of-the-collembola-hexapoda-spermatozoa
#17
P P Fanciulli, C Mencarelli, D Mercati, R Dallai, P Lupetti
The springtail Collembola are characterized by having rolled spermatozoa, with a long cylindrical extracellular structure adhering to the acrosome. This structure is produced by the secretory activity of the testes epithelial cells at almost the end of spermiogenesis. At the beginning of its formation, it is a thin extension with a helical wall and a dense axial region. Later the cylindrical structure shows an inner organization which is different in the several species examined: species of Entomobryidae contain material with a paracrystalline structure, whilst some of Symphypleona contain ovoid structures...
June 29, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28708510/changes-in-localization-and-density-of-cd63-positive-exosome-like-substances-in-the-hen-oviduct-with-artificial-insemination-and-their-effect-on-sperm-viability
#18
A Huang, N Isobe, Y Yoshimura
Avian sperm are stored in the sperm storage tubules (SSTs) of the hen oviduct for a prolonged period. However, the precise mechanisms by which sperm are kept alive in the SSTs are still not fully understood. The aim of this study was to determine whether exosomes are secreted by SST cells and play a role in the survival of sperm. Utero-vaginal junction (UVJ) tissue from approximately 50 wk old White Leghorn hens was collected before (control group) and after intravaginal insemination with seminal plasma (SP group) or semen (AI group)...
June 29, 2017: Theriogenology
https://www.readbyqxmd.com/read/28692759/sperm-morphology-assessment-pathophysiology-clinical-relevance-and-state-of-the-art-in-2017
#19
REVIEW
N Gatimel, J Moreau, J Parinaud, R D Léandri
For over 30 years, sperm morphology assessment has been one of the most common tests in evaluation of fertility. This review examines the clinical relevance of sperm morphology assessment in the diagnosis of infertility and in assisted reproductive technology, as well as its analytical reliability. Publications on the pathophysiology, the analytical reliability of the test and its clinical relevance in diagnosis and in Assisted Reproductive Technology (ART) were evaluated. This review compared and discussed study methodologies and results, including patient characteristics, preparation, smear staining methods and classification systems...
September 2017: Andrology
https://www.readbyqxmd.com/read/28678425/semi-automated-scoring-of-triple-probe-fish-in-human-sperm-using-confocal-microscopy
#20
Francesca Branch, GiaLinh Nguyen, Nicholas Porter, Heather A Young, Sheena E Martenies, Nathan McCray, Glen Deloid, Anastas Popratiloff, Melissa J Perry
Structural and numerical sperm chromosomal aberrations result from abnormal meiosis and are directly linked to infertility. Any live births that arise from aneuploid conceptuses can result in syndromes such as Kleinfelter, Turners, XYY and Edwards. Multi-probe fluorescence in situ hybridization (FISH) is commonly used to study sperm aneuploidy, however manual FISH scoring in sperm samples is labor-intensive and introduces errors. Automated scoring methods are continuously evolving. One challenging aspect for optimizing automated sperm FISH scoring has been the overlap in excitation and emission of the fluorescent probes used to enumerate the chromosomes of interest...
July 5, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
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