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https://www.readbyqxmd.com/read/29136144/antioxidants-improve-ivf-outcome-and-subsequent-embryo-development-in-the-mouse
#1
T Truong, D K Gardner
STUDY QUESTION: What is the effect of a combination of three antioxidants (Acetyl-L-Carnitine, N-Acetyl-L-Cysteine and α-Lipoic Acid), present in IVF medium during mouse oocyte and sperm collection, on fertilization and subsequent IVF embryo development? SUMMARY ANSWER: A combination of antioxidants resulted in faster developmental times from the 2-cell stage through to expanded blastocyst stage, accompanied by a significant increase in blastocyst cell number and a reduction of intracellular hydrogen peroxide (H2O2) levels...
November 10, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29110260/disparities-in-reproductive-outcomes-according-to-the-endometrial-preparation-protocol-in-frozen-embryo-transfer-the-risk-of-early-pregnancy-loss-in-frozen-embryo-transfer-cycles
#2
I Hatoum, L Bellon, N Swierkowski, M Ouazana, S Bouba, K Fathallah, B Paillusson, M Bailly, F Boitrelle, L Alter, M Bergère, J Selva, R Wainer
PURPOSE: The purpose of this study was to determine the effect of stimulated and artificial endometrial preparation protocols on reproductive outcomes in frozen embryo transfer (FET) cycles. METHODS: We performed a retrospective study of 1926 FET cycles over a 3.5-year period in the Fertility Unit at a University Hospital. Stimulated and artificial protocols were used for endometrial preparation. The embryos for FET were obtained from either in vitro fertilization or intracytoplasmic sperm injection cycles...
November 6, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29108863/alterations-in-sperm-dna-methylation-patterns-of-oligospermic-males
#3
Mohammed Laqqan, Sascha Tierling, Yaser Alkhaled, Christina LoPorto, Mohamad Eid Hammadeh
Aberrant in sperm DNA methylation patterns and histone modification play a role in male fecundity decline. This study was prepared to determine whether sperm DNA methylation at CpG dinucleotides is different in oligospermic males compared to proven fertile males and then to evaluate the correlation between the changes in sperm DNA methylation patterns and semen parameters of oligospermic males. A total of 165 males (64 proven fertile males "controls" and 101 oligospermic males "cases") were included in the study...
November 3, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/29108134/production-of-recombinant-human-tektin-1-2-and-4-and-in-vitro-assembly-of-human-tektin-1
#4
M S Budamagunta, F Guo, N Sun, B Shibata, P G FitzGerald, J V Voss, J F Hess
Proteins predicted to be composed of large stretches of coiled-coil structure have often proven difficult to crystallize for structural determination. We have successfully applied EPR spectroscopic techniques to the study of the structure and assembly of full-length human vimentin assembled into native 11 nm filaments, in physiologic solution, circumventing the limitations of crystallizing shorter peptide sequences. Tektins are a small family of highly alpha helical filamentous proteins found in the doublet microtubules of cilia and related structures...
November 6, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/29107676/perinatal-exposure-to-low-dose-2-2-4-4-tetrabromodiphenyl-ether-bde-47-alters-sperm-dna-methylation-in-adult-rats
#5
Alexander Suvorov, Alex Shershebnev, Haotian Wu, Yulia Medvedeva, Oleg Sergeyev, J Richard Pilsner
Polybrominated diphenyl ethers (PBDEs) are a group of ubiquitous reproductive toxins. Given that spermatogenesis requires extensive epigenetic changes, we hypothesize that PBDEs impact sperm DNA methylation. Pregnant Wistar rats were exposed perinatally to 0.2mg/kg 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and caudal epididymal sperm were collected from offspring on postnatal days (PNDs) 65 and 120. Libraries were prepared from sperm DNA and sequenced with an average of 18.0 million unique reads per sample...
October 28, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/29034050/separation-of-sperm-cells-from-samples-containing-high-concentrations-of-white-blood-cells-using-a-spiral-channel
#6
Jiyoung Son, Raheel Samuel, Bruce K Gale, Douglas T Carrell, James M Hotaling
Microfluidic technology has potential to separate sperm cells from unwanted debris while improving the effectiveness of assisted reproductive technologies (ART). Current clinical protocol limitations regarding the separation of sperm cells from other cells/cellular debris can lead to low sperm recovery when the sample contains a low concentration of mostly low motility sperm cells and a high concentration of unwanted cells/cellular debris, such as in semen samples from patients with pyospermia [high white blood cell (WBC) semen]...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28990130/sperm-preparation-after-freezing-improves-motile-sperm-count-motility-and-viability-in-frozen-thawed-sperm-compared-with-sperm-preparation-before-freezing-thawing-process
#7
A Palomar Rios, A Gascón, J V Martínez, S Balasch, I Molina Botella
PURPOSE: The aim of this study is to evaluate which cryopreservation protocol, freezing before or after swim-up, optimizes cryopreservation outcomes in terms of motile sperm count, motility, morphology, and viability, and also to establish whether sperm viability could be assessed based on sperm motility. METHODS: Fifty-three fresh and 53 swim-up prepared samples were considered for the first experiment. In parallel, total motility evaluation by CASA system (computer-assisted sperm analyzer) and hypoosmotic swelling test (HOS-test) was performed in each sample to compare the viability results of both methods...
October 9, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28965409/characterization-of-the-key-aroma-compounds-in-white-alba-truffle-tuber-magnatum-pico-and-burgundy-truffle-tuber-uncinatum-by-means-of-the-sensomics-approach
#8
Philipp C Schmidberger, Peter Schieberle
Aroma extract dilution analysis of distillates prepared by solvent extraction and solvent-assisted flavor evaporation distillation from white Alba truffle (WAT; Tuber magnatum pico) and Burgundy truffle (BT; Tuber uncinatum) revealed 20 odor-active regions in the flavor dilution (FD) factor range of 16-4096 in WAT and 25 in BT. The identification experiments in combination with the FD factors showed clear differences in the overall set of key odorants of both fungi. While 3-(methylthio)propanal (potato-like) followed by 2- and 3-methylbutanal (malty), 2,3-butanedione (buttery), and bis(methylthio)methane (garlic-like) showed the highest FD factors in WAT, 2,3-butanedione, phenylacetic acid (honey-like), and vanillin (vanilla-like) had the highest FD factors in BT...
October 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28929253/positive-rheotaxis-extended-drop-a-one-step-procedure-to-select-and-recover-sperm-with-mature-chromatin-for-intracytoplasmic-sperm-injection
#9
Hamilton De Martin, Marcello S Cocuzza, Bruno C Tiseo, Guilherme J A Wood, Eduardo P Miranda, Pedro A A Monteleone, José Maria Soares, Paulo C Serafini, Miguel Srougi, Edmund C Baracat
PURPOSE: The purpose of this study was to develop a novel one-step ICSI approach to select sperm with better chromatin maturity than the conventional method. METHODS: This was a pilot diagnostic study, which prospectively recruited men during a 6-month period in a University-affiliated infertility centre. Forty consecutive semen samples were provided for analysis. The positive rheotaxis extended drop (PRED) was set up creating a pressure and viscosity gradient. Each semen sample was divided into four aliquots: one aliquot for density gradient centrifugation (DGC), two aliquots for PRED (fresh semen (PRED-FS) and processed semen (PRED-DGC)), and one aliquot as the control (FS)...
September 19, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28904906/a-step-by-step-guide-to-office-based-sperm-retrieval-for-obstructive-azoospermia
#10
REVIEW
Robert M Coward, Jesse N Mills
A variety of surgical options exists for sperm retrieval in the setting of obstructive azoospermia (OA). With appropriate preparation, the majority of these techniques can safely be performed in the office with local anesthesia and with or without monitored anesthesia care (MAC). The available techniques include percutaneous options such as percutaneous epididymal sperm aspiration (PESA) and testicular sperm aspiration (TESA), as well as open techniques that include testicular sperm extraction (TESE) and microsurgical epididymal sperm aspiration (MESA)...
August 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28904502/antispermatogenic-mechanism-of-trona-is-associated-with-lipid-peroxidation-but-not-testosterone-suppression
#11
Ayodeji F Ajayi, Roland E Akhigbe
BACKGROUND: About half of the cases of infertility in couples have been attributed to male factor. Despite the claim in folklore medicine that trona (a sesquicarbonate or hydrated carbonate of sodium) causes fetal loss, its effect on male reproductive function has not been investigated. AIM: This study sought to provide scientific evidence on the effect of trona on sperm characteristics, male reproductive hormones and organs, and lipid peroxidation. MATERIALS AND METHODS: Forty male Wistar rats of comparable weights were used for the study...
April 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/28878960/eversion-and-withdrawal-of-an-intromittent-organ-before-sexual-maturation-prepares-male-beetles-for-copulation
#12
Yoko Matsumura, Takuya Kubo
Some species of criocerine beetles have a hyper-elongated part of the intromittent organ called a flagellum. In resting position, the flagellum is stored in a specialized internal sac in the intromittent organ. This specialized state of the flagellum and internal sac is indispensable during copulation for flagellar insertion into the female spermathecal duct for sperm transfer. However, the morphogenesis of the flagellum does not generate the active state of the flagellum; rather, the flagellum is generated in an inactive and completely coiled state...
August 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28869110/anandamide-exerts-a-suppressive-effect-on-sperm-binding-to-oviduct-explants-through-cb1-receptors-in-the-water-buffalo-bubalus-bubalis
#13
Vimlesh Kumar, Arumugam Kumaresan, Puneeth Kumar D S, Sreela Lathika, Samiksha Nayak, Kaustubh Kishor Saraf, Pradeep Nag B S, Shivani Chhillar, Tirtha Kumar Datta, Tushar Kumar Mohanty
An endocannabinoid system comprising of Anandamide (AEA) and its receptor has been shown to play a role in sperm acquisition of fertilizing potential and sperm-oviduct interaction. In the present study, we assessed the effect of sperm pre-treatment with AEA or co-incubation of sperm-oviduct explants with AEA in the presence or absence of CB1 receptor antagonist (SR141716A) on sperm-oviduct binding in the water buffalo. Cryopreserved spermatozoa from 3 Murrah buffalo bulls (3 ejaculates from each bull) were utilized for the study...
October 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28868247/vitrification-increased-vacuolization-of-human-spematozoa-application-of-msome-technology
#14
Sara Taherzadeh, Mohammad Ali Khalili, Azam Agha-Rahimi, Fateme Anbari, Shahin Ghazali, Guido Macchiarelli
BACKGROUND: Sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (LN2). The aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by MSOME technology and the fertility potential by zona binding assay (ZBA). METHODS: 20 normo-ejaculates were prepared by swim up technique, and supernatants were divided into two parts of fresh and vitrified groups...
April 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28854719/techniques-used-for-iui-is-it-time-for-a-change
#15
L Lemmens, S Kos, C Beijer, D D M Braat, W L D M Nelen, A M M Wetzels
STUDY QUESTION: Are the guidelines for the technical aspects of IUI (WHO, 2010) still in accordance with the current literature? SUMMARY ANSWER: In general, the laboratory guidelines of the World Health Organization (WHO) are a suitable protocol, although the evidence is not always conclusive and some changes are advisable. WHAT IS KNOWN ALREADY: Lack of standardization of the technical procedures required for IUI might result in inter-laboratory variation in pregnancy rates...
September 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28853925/sex-determination-in-the-mammalian-germline
#16
Cassy Spiller, Peter Koopman, Josephine Bowles
Sexual reproduction crucially depends on the production of sperm in males and oocytes in females. Both types of gamete arise from the same precursor, the germ cells. We review the events that characterize the development of germ cells during fetal life as they commit to, and prepare for, oogenesis or spermatogenesis. In females, fetal germ cells enter meiosis, whereas in males they delay meiosis and instead lose pluripotency, activate an irreversible program of prospermatogonial differentiation, and temporarily cease dividing...
August 30, 2017: Annual Review of Genetics
https://www.readbyqxmd.com/read/28836411/evidence-of-oocyte-polarity-in-bovine-implications-for-intracytoplasmic-sperm-injection-and-somatic-cell-nuclear-transfer
#17
Seyed Morteza Hosseini, Fariba Moulavi, Nima TanhaieVash, Naser Shams-Esfandabadi, Mohammad Hossein Nasr-Esfahani, Abolfazl Shirazi
OBJECTIVES: We recently demonstrated spatial regionalization of maternal transcripts and proteins within unfertilized ovine oocyte. Here, we investigated the likelihood of oocyte polarity for the first time in bovine. MATERIALS AND METHODS: In this experimental study, in vitro matured bovine oocytes were used for manual bisection [into oocyte halve that were near-to (HNS) and far-from (FS) spindle] or trisection [into MII-spindle (S), the spindle-side half (NS), and the distal half unassociated with the spindle (FS)]...
October 2017: Cell Journal
https://www.readbyqxmd.com/read/28830817/evaluation-of-the-aphrodisiac-potential-of-a-chemically-characterized-aqueous-extract-of-tamarindus-indica-pulp
#18
Amita Rai, Snehashis Das, Mallikarjuna Rao Chamallamudi, Krishnadas Nandakumar, Raghavendra Shetty, Meghna Gill, Suhani Sumalatha, Raviraj Devkar, Karthik Gourishetti, Nitesh Kumar
ETHNOPHARMACOLOGICAL RELEVANCE: Tamarindus indica is an ingredient in the traditional aphrodisiac formulations in Africa and India. It is also a widely used food ingredient in other tropical countries. AIM OF THE STUDY: The present study was aimed to evaluate the aphrodisiac potential and reproductive safety profile of aqueous extract of Tamarindus indica in male Wistar rats. MATERIALS AND METHODS: The aqueous extract was prepared by maceration of pulp followed by reduction of volume in rotavapor under heat followed by freeze drying...
August 19, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28815355/egg-donation-of-vitrified-oocytes-bank-produces-similar-pregnancy-rates-by-blastocyst-transfer-when-compared-to-fresh-cycle
#19
Thais S Domingues, Ana Paula Aquino, Bruna Barros, Raquel Mazetto, Mariana Nicolielo, Carolina M Kimati, Talita Devecchi, Tatiana C S Bonetti, Paulo C Serafini, Eduardo L A Motta
PURPOSE: Advances in reproductive techniques, mainly the introduction of oocyte vitrification, have provided the opportunity to conceive from oocyte banks. The aim of this study was to compare the clinical outcomes of fresh and vitrified oocytes in an egg donation program following blastocyst transfer. METHODS: This retrospective observational study included 504 oocyte donation cycles. All donor women were younger than 30 years of age. The recipient cycles were divided into two groups: fresh oocytes (n = 78) or vitrified oocytes (n = 426)...
August 16, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28765033/assessment-of-butorphanol-azaperone-medetomidine-combination-as-anesthesia-for-semen-collection-and-evaluation-of-semen-quality-in-white-tailed-deer-odocoileus-virginianus
#20
S M Kirschner, R Rodenkirch
The aim of this current study was to evaluate the level of anesthesia produced by a combination of butorphanol-azaperone-medetomidine (BAM) for semen collection by electroejaculation on captive white-tailed bucks (Odocoileus virginianus). Ten male white-tailed deer, weighing 68.2-115.9kg, ranging in age from one to four years were randomly selected from housing pens and anesthetized with the BAM drug combination at a dose volume of 2.0mL each. Semen was collected from each animal using a standard cervid electroejaculation protocol while under BAM anesthesia...
July 25, 2017: Animal Reproduction Science
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