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IVF culture media

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https://www.readbyqxmd.com/read/29725911/continuous-embryo-culture-elicits-higher-blastulation-but-similar-cumulative-delivery-rates-than-sequential-a-large-prospective-study
#1
Danilo Cimadomo, C Scarica, R Maggiulli, G Orlando, D Soscia, L Albricci, S Romano, F Sanges, F M Ubaldi, L Rienzi
PURPOSE: To assess whether continuous embryo culture involves better embryological and/or clinical outcomes than sequential. METHODS: Prospective study at a private IVF center. All consecutive IVF cycles (September 2013-2015) fulfilling the inclusion criteria underwent embryo culture in either Continuous-Single-Culture-Media (CSCM, n = 972) or sequential media (Quinn's Advantage, n = 514), respectively. ICSI, blastocyst culture in either standard (MINC) or undisturbed (Embryoscope) incubation, transfer (until September 2016), and pregnancy follow-up (until September 2017) were performed...
May 3, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29709567/-culture-conditions-for-gametes-and-embryos-which-culture-medium-which-impact-on-newborn
#2
I Koscinski, M Merten, N Kazdar, J-L Guéant
Many studies have examined the impact of cell/embryo culture media on the development of human embryo during IVF process, but few studies have followed up and compared the effects of these culture media on the developmental outcome of children conceived by IVF. As recurrent experimental evidence from animal studies suggests potential long-term effects of embryo culture media on the health outcome of IVF-conceived children, more studies are needed to clarify the role of the culture media and mechanisms underlying such effects...
April 27, 2018: Gynecologie, Obstetrique, Fertilite & Senologie
https://www.readbyqxmd.com/read/29664793/extracellular-vesicles-a-promising-tool-for-assessment-of-embryonic-competence
#3
Diego Marin, Richard T Scott
PURPOSE OF REVIEW: Extracellular vesicles have recently emerged as a promising field of research due to their pivotal roles in intercellular communication and potential to serve as biomarkers. This review focuses on extracellular vesicles secreted by the human preimplantation embryo. The most recent findings on embryo-derived extracellular vesicles are described and discussed, as well as current technical challenges to study them. RECENT FINDINGS: So far, only a few studies have addressed extracellular vesicles of embryonic origin and explored their potential as biomarkers for embryo selection...
June 2018: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29626464/impact-of-l-carnitine-on-lipid-content-and-post-thaw-survivability-of-buffalo-embryos-produced-in-vitro
#4
Megha Verma, Sriti Pandey, Irfan A Bhat, B Mukesh, J Anand, Vikash Chandra, G Taru Sharma
The aim of the present study was to see the impact of L-Carnitine (LC) on lipid biosynthesis and metabolism of buffalo embryos, and post thawed blastocyst survivability. In vitro fertilized (IVF) embryos were derived from slaughterhouse derived COCs and cultured in different doses of LC i.e. 0, 1 mM, 1.5 mM, 2 mM starting at 48 h post IVF. Blastocyst rate was significantly (p < 0.05) higher in 1.5 mM group than control and 1.0 mM group. Lipid content was measured indirectly by fluorescent intensity of lipid droplets after Nile red staining, and it was lower (p < 0...
April 4, 2018: Cryobiology
https://www.readbyqxmd.com/read/29587610/ngs-analysis-of-human-embryo-culture-media-reveals-mirnas-of-extra-embryonic-origin
#5
Immaculada Sánchez-Ribas, Patricia Diaz-Gimeno, Alicia Quiñonero, María Ojeda, Zaloa Larreategui, Agustín Ballesteros, Francisco Domínguez
Our objective in this work was to isolate, identify, and compare micro-RNAs (miRNAs) found in spent culture media of euploid and aneuploid in vitro fertilization (IVF) embryos. Seventy-two embryos from 62 patients were collected, and their spent media were retained. A total of 108 spent conditioned media samples were analyzed (n = 36 day 3 euploid embryos, n = 36 day 3 aneuploid embryos, and n = 36 matched control media). Fifty hed-control media embryos were analyzed using next-generation sequencing (NGS) technology...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29538675/determinants-of-monozygotic-twinning-in-art-a-systematic-review-and-a-meta-analysis
#6
Kathrine Vauvert R Hviid, Sara Sofia Malchau, Anja Pinborg, Henriette Svarre Nielsen
BACKGROUND: The incidence of monozygotic twins (MZT) after ART appears to be higher than the incidence after spontaneous conceptions contradicting the aim of ART to avoid multiple pregnancies because of the associated risks. OBJECTIVE AND RATIONALE: The aim was to study the frequency of MZT after IVF and ICSI and how it is influenced by the day of embryo transfer, maternal age, zona pellucida manipulation, controlled ovarian stimulation, stimulation protocol, culture media and embryo quality...
March 12, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29531831/common-medium-versus-advanced-ivf-medium-for-cryopreserved-oocytes-in-heterologous-cycles
#7
R Poverini, R Lisi, F Lisi, V Berlinghieri, W Bielli, P Carfagna, A Costantino, D Iacomino, G Nicodemo
Granulocyte-macrophage colony-stimulation factor plays different crucial roles during embryo implantation and subsequent development. Here we aimed to evaluate the effects of embryo cell culture medium, with the inclusion of granulocyte-macrophage colony-stimulation factor (GM-CSF), on embryo development and pregnancy rate. To this end, we took advantage of our retrospective observational study to correlate the outcomes from two different culture media. We included in this study 25 unselected patient from our IVF Center that underwent heterologous IVF cycle with crypreserved oocytes...
December 2018: Cell Death Discovery
https://www.readbyqxmd.com/read/29396043/characteristics-and-fertility-of-domestic-cat-epididymal-spermatozoa-cryopreserved-with-two-different-freezing-media
#8
Eugeny Brusentsev, Elena Kizilova, Valentina Mokrousova, Valeria Kozhevnikova, Irina Rozhkova, Sergei Amstislavsky
The study represents a comparison of cryopreservation of domestic cat epididymal spermatozoa with two commercially available freezing media: CaniPlus Freeze (CPF) and SpermFreeze (SF). The viability of nonfrozen spermatozoa evaluated by the VitalScreen test was 68.7 ± 3.0%. These figures were lower for the frozen-thawed spermatozoa: 51.2 ± 6.3% for CPF group and 54.4 ± 3.1% for SF group. The motility of nonfrozen spermatozoa was 57.2 ± 4.5%. These figures were reduced in both frozen-thawed groups; however, there was no significant difference in these parameters between CPF (30...
April 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/29325603/culture-conditions-affect-ca2-release-in-artificially-activated-mouse-and-human-oocytes
#9
Yuechao Lu, Davina Bonte, Minerva Ferrer-Buitrago, Mina Popovic, Jitesh Neupane, Margot Van der Jeught, Luc Leybaert, Petra De Sutter, Björn Heindryckx
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the pattern of Ca2+ release and embryonic developmental potential. In the mouse, application of 5μM ionomycin in potassium simplex optimisation medium (KSOM) or 10µM ionomycin in Ca2+-free KSOM significantly reduced the Ca2+ flux and resulted in failure of blastocyst formation compared with 10μM ionomycin in KSOM...
January 12, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29314770/calibration-of-redox-potential-in-sperm-wash-media-and-evaluation-of-oxidation-reduction-potential-values-in-various-assisted-reproductive-technology-culture-media-using-mioxsys-system
#10
M K Panner Selvam, R Henkel, R Sharma, A Agarwal
Oxidation-reduction potential describes the balance between the oxidants and antioxidants in fluids including semen. Various artificial culture media are used in andrology and IVF laboratories for sperm preparation and to support the development of fertilized oocytes under in vitro conditions. The composition and conditions of these media are vital for optimal functioning of the gametes. Currently, there are no data on the status of redox potential of sperm processing and assisted reproduction media. The purpose of this study was to compare the oxidation-reduction potential values of the different media and to calibrate the oxidation-reduction potential values of the sperm wash medium using oxidative stress inducer cumene hydroperoxide and antioxidant ascorbic acid...
March 2018: Andrology
https://www.readbyqxmd.com/read/29301092/metabolomic-profiling-of-bovine-oviductal-fluid-across-the-oestrous-cycle-using-proton-nuclear-magnetic-resonance-spectroscopy
#11
Julie Lamy, Julie Gatien, Florine Dubuisson, Lydie Nadal-Desbarats, Pascal Salvetti, Pascal Mermillod, Marie Saint-Dizier
In the present study we tested whether regulation of the metabolome in bovine oviductal fluid depended on the stage of the oestrous cycle, the side relative to ovulation and local concentrations of steroid hormones. Luminal fluid samples from both oviducts were collected in the preovulatory, postovulatory, mid- and late luteal phases, from cyclic cows at a local abattoir (18-27 cows per stage and side). The metabolomes were assessed by proton nuclear magnetic resonance spectroscopy (H-NMR). In all, 39 metabolites were identified, among which the amino acid glycine and the energy substrates lactate and myoinositol were the most abundant at all stages...
January 5, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29234954/low-versus-high-volume-of-culture-medium-during-embryo-transfer-a-randomized-clinical-trial
#12
George Α Sigalos, Yannis Michalopoulos, Athanasios G Kastoras, Olga Triantafyllidou, Nikos F Vlahos
PURPOSE: The aim of this prospective randomized control trial was to evaluate if the use of two different volumes (20-25 vs 40-45 μl) of media used for embryo transfer affects the clinical outcomes in fresh in vitro fertilization (IVF) cycles. METHODS: In total, 236 patients were randomized in two groups, i.e., "low volume" group (n = 118) transferring the embryos with 20-25 μl of medium and "high volume" group (n = 118) transferring the embryos with 40-45 μl of medium...
December 12, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29197293/synergistic-effect-of-cysteamine-leukemia-inhibitory-factor-and-y27632-on-goat-oocyte-maturation-and-embryo-development-in-vitro
#13
Liyou An, Jiao Liu, Yinyan Du, Zhihui Liu, Fenli Zhang, Yanhong Liu, Xiumei Zhu, Pingping Ling, Shiwei Chang, Yeshu Hu, Yi Li, Bowen Xu, Lan Yang, Fei Xue, Giorgio Antonio Presicce, Fuliang Du
Goat oocyte in vitro maturation is associated with a variable efficiency of embryo development after in vitro fertilization (IVF). Here, we developed a novel maturation procedure to evaluate the cellular effect of cysteamine (Cys), leukemia inhibitory factor (LIF) and Y27632 on oocyte in vitro maturation in native Chinese Yangtze river white goats. Oocytes were collected by slicing ovary tissues and matured for 24 h in vitro prior to IVF. Presumptive fertilized oocytes were cultured in embryo media for 8 days...
March 1, 2018: Theriogenology
https://www.readbyqxmd.com/read/29177442/increased-27-hydroxycholesterol-production-during-luteolysis-may-mediate-the-progressive-decline-in-progesterone-secretion
#14
Yafei Xu, Scot M Hutchison, José J Hernández-Ledezma, Randy L Bogan
STUDY QUESTION: Does 27-hydroxycholesterol (27OH) actively facilitate the progression of luteolysis? SUMMARY ANSWER: There is increased mRNA expression of the enzyme that produces 27OH during luteolysis in vivo in rhesus macaques and sheep, and 27OH reduces progesterone secretion from human luteinized granulosa cells. WHAT IS KNOWN ALREADY: There is an increase in mRNA expression of liver x receptor (LXR) and a decrease in sterol regulatory element binding protein 2 (SREBP2) target genes during spontaneous luteolysis in primates, which could result in reduced cholesterol availability for steroidogenesis...
January 1, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29152322/choosing-a-culture-medium-for-scnt-and-iscnt-reconstructed-embryos-from-domestic-to-wildlife-species
#15
REVIEW
A Cordova, W A King, G F Mastromonaco
Over the past decades, in vitro culture media have been developed to successfully support IVF embryo growth in a variety of species. Advanced reproductive technologies, such as somatic cell nuclear transfer (SCNT), challenge us with a new type of embryo, with special nutritional requirements and altered physiology under in vitro conditions. Numerous studies have successfully reconstructed cloned embryos of domestic animals for biomedical research and livestock production. However, studies evaluating suitable culture conditions for SCNT embryos in wildlife species are scarce (for both intra- and interspecies SCNT)...
2017: Journal of Animal Science and Technology
https://www.readbyqxmd.com/read/29142449/single-blastocyst-transfer-the-key-to-reduce-multiple-pregnancy-rates-without-compromising-the-live-birth-rate
#16
Uma M Sundhararaj, Monali V Madne, Reeta Biliangady, Sumana Gurunath, Ambika G Swamy, Indu S T Gopal
Background: Historically, to achieve higher pregnancy rates, multiple embryos were transferred after an in-vitro fertilisation (IVF). However, this practice is being reassessed, because it leads to multiple pregnancies that is known to cause adverse maternal and fetal outcomes. Aim: To compare the pregnancy outcomes in fresh IVF or intracytoplasmic sperm injection (ICSI) cycles among women undergoing elective single blastocyst transfer (eSBT) vs. those undergoing double blastocyst transfer (DBT)...
July 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29136144/antioxidants-improve-ivf-outcome-and-subsequent-embryo-development-in-the-mouse
#17
T Truong, D K Gardner
STUDY QUESTION: What is the effect of a combination of three antioxidants (Acetyl-L-Carnitine, N-Acetyl-L-Cysteine and α-Lipoic Acid), present in IVF medium during mouse oocyte and sperm collection, on fertilization and subsequent IVF embryo development? SUMMARY ANSWER: A combination of antioxidants resulted in faster developmental times from the 2-cell stage through to expanded blastocyst stage, accompanied by a significant increase in blastocyst cell number and a reduction of intracellular hydrogen peroxide (H2O2) levels...
December 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29099693/comparative-analysis-of-sperm-selection-methods-before-the-intra-cytoplasmic-sperm-injection-procedure
#18
COMPARATIVE STUDY
O Lohinova, V Pitko, N Sinilo, Ya Cherkashyna, N Hanzha
In recent years, one of the methods of assisted reproductive technologies has become increasingly widespread, namely the ICSI procedure (ICSI - Intra Cytoplasmic Sperm Injection). This method is based on artificial introduction of the spermatozoon into the cytoplasm of the oocyte. However, the complexity of this method is that the choice of a morphologically and biochemically mature spermatozoon becomes subjective. However, not so long ago, an innovative method for selecting mature, ready-to-fertilize spermatozoa was proposed...
October 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28938894/in-vitro-progesterone-production-by-luteinized-human-mural-granulosa-cells-is-modulated-by-activation-of-ampk-and-cause-of-infertility
#19
E C Bowdridge, M W Vernon, J A Flores, M J Clemmer
BACKGROUND: Mural granulosa cells from IVF patients were provided by the West Virginia University Center for Reproductive Medicine in Morgantown, WV. The effect of adenosine monophosphate activated protein kinase (AMPK) activation, primary cause of infertility, age, BMI, and pregnancy outcome on production of progesterone were examined separately. METHODS: Isolated mural sheets from IVF patients (n = 26) were centrifuged, supernatant discarded, and the pellet re-suspended in 500 μl of DMEM/F12...
September 22, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28851827/-mg2-o-ca2-o-determines-ca2-response-at-fertilization-tuning-of-adult-phenotype
#20
Jean-Pierre Ozil, Thierry Sainte-Beuve, Bernadette Banrezes
Alteration of the postnatal phenotype has sparked great concern about the developmental impact of culture media used at fertilization. However, the mechanisms and compounds involved are yet to be determined. Here we used the Ca2+ responses from mouse eggs fertilized by ICSI as a dynamic and quantitative marker to understand the role of compounds in egg functioning and establish possible correlations with adult phenotypes. We computed 134 Ca2+ responses from the first to the last oscillation in media with specific formulations...
August 29, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
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