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IVF culture media

E Dietzel, J Floehr, E Van de Leur, R Weiskirchen, W Jahnen-Dechent
STUDY QUESTION: Does fetuin-B inhibit premature zona pellucida (ZP) hardening in mouse oocytes in vitro and thus increase IVF rate? SUMMARY ANSWER: Supplementation of oocyte in vitro maturation (IVM) media with recombinant mouse fetuin-B (rmFetuB) increased fertilization rate without affecting mouse embryo development into blastocysts. WHAT IS KNOWN ALREADY: Mice deficient in fetuin-B are infertile owing to premature ZP hardening. Premature ZP hardening also occurs during oocyte IVM leading to decreased fertilization rate...
October 12, 2016: Molecular Human Reproduction
Séverine Le Gac, Verena Nordhoff
The optimization of in-vitro culture conditions and the selection of the embryo(s) with the highest developmental competence are essential components in an ART program. Culture conditions are manifold and they underlie not always evidence-based research but also trends entering the IVF laboratory. At the moment, the idea of using sequential media according to the embryo requirements has been given up in favor of the use of single step media in an uninterrupted manner due to practical issues such as time-lapse incubators...
September 27, 2016: Molecular Human Reproduction
Fang Gu, Mingfen Deng, Jun Gao, Zilian Wang, Chenhui Ding, Yanwen Xu, Canquan Zhou
BACKGROUND: Embryo culture media used for IVF treatment might affect fetal growth and thus birthweight of the newborns. METHODS: A retrospective study was conducted in South China using data from 2370 singleton neonates born after IVF/ICSI between 2009 and 2012. Two culture media, i.e., either Vitrolife or SAGE were used as embryo culture media during the study period. Neonates' birthweights were compared between the two embryo culture media groups. RESULTS: Among the 2370 singletons, 1755 cases came from fresh cleavage embryo transfer while 615 were from frozen-thawed cleavage embryo transfer...
2016: BMC Pregnancy and Childbirth
Mousa I Shamonki, Helen Jin, Zachary Haimowitz, Lian Liu
OBJECTIVE: To assess whether preimplantation genetic screening (PGS) is possible by testing for free embryonic DNA in spent IVF media from embryos undergoing trophectoderm biopsy. DESIGN: Prospective cohort analysis. SETTING: Academic fertility center. PATIENT(S): Seven patients undergoing IVF and 57 embryos undergoing trophectoderm biopsy for PGS. INTERVENTION(S): On day 3 of development, each embryo was placed in a separate media droplet...
August 23, 2016: Fertility and Sterility
Mohammad Mehdi Naderi, Sara Borjian Boroujeni, Ali Sarvari, Banafsheh Heidari, Mohammad Mehdi Akhondi, Amir-Hassan Zarnani, Abolfazl Shirazi
BACKGROUND: This study was aimed to assess the effects of angiotensin II (Ang II) supplementation to the In Vitro Maturation (IVM) and In Vitro Culture (IVC) media of vitrified-warmed ovine oocytes on their developmental competence and expression of Na(+)/K(+)/ATPase in resulting embryos. METHODS: The slaughterhouse-derived immature oocytes (n=1069) were randomly distributed into four experimental groups: groups I and II) IVM/IVF and IVC of fresh and vitrified oocytes without angiotensin supplementation (Control-Fresh and Control-Vit groups, respectively); group III) IVM of vitrified oocytes in the presence of Ang II followed by IVF/IVC (Vit-IVM group); and group IV) IVM/IVF of vitrified oocytes followed by IVC wherein the embryos were exposed to Ang II on day 4 of IVC (Vit-D4 group)...
July 2016: Avicenna Journal of Medical Biotechnology
Sander H M Kleijkers, Eleni Mantikou, Els Slappendel, Dimitri Consten, Jannie van Echten-Arends, Alex M Wetzels, Madelon van Wely, Luc J M Smits, Aafke P A van Montfoort, Sjoerd Repping, John C M Dumoulin, Sebastiaan Mastenbroek
STUDY QUESTION: Does embryo culture medium influence pregnancy and perinatal outcome in IVF? SUMMARY ANSWER: Embryo culture media used in IVF affect treatment efficacy and the birthweight of newborns. WHAT IS KNOWN ALREADY: A wide variety of culture media for human preimplantation embryos in IVF/ICSI treatments currently exists. It is unknown which medium is best in terms of clinical outcomes. Furthermore, it has been suggested that the culture medium used for the in vitro culture of embryos affects birthweight, but this has never been demonstrated by large randomized trials...
October 2016: Human Reproduction
Ioannis A Sfontouris, Wellington P Martins, Carolina O Nastri, Iara G R Viana, Paula A Navarro, Nick Raine-Fenning, Sheryl van der Poel, Laura Rienzi, Catherine Racowsky
PURPOSE: The purpose of this study was to undertake a review of the available evidence comparing the use of a single medium versus sequential media for embryo culture to the blastocyst stage in clinical IVF. METHODS: We searched the Cochrane Central, PubMed, Scopus,, Current Controlled Trials and WHO International Clinical Trials Registry Platform to identify randomized controlled trials comparing single versus sequential media for blastocyst culture and ongoing pregnancy rate...
October 2016: Journal of Assisted Reproduction and Genetics
Antonio Capalbo, Valeria Romanelli, Danilo Cimadomo, Laura Girardi, Marta Stoppa, Lisa Dovere, Domenico Dell'Edera, Filippo Maria Ubaldi, Laura Rienzi
For an IVF clinic that wishes to implement preimplantation genetic diagnosis for monogenic diseases (PGD) and for aneuploidy testing (PGD-A), a global improvement is required through all the steps of an IVF treatment and patient care. At present, CCS (Comprehensive Chromosome Screening)-based trophectoderm (TE) biopsy has been demonstrated as a safe, accurate and reproducible approach to conduct PGD-A and possibly also PGD from the same biopsy. Key challenges in PGD/PGD-A implementation cover genetic and reproductive counselling, selection of the most efficient approach for blastocyst biopsy as well as of the best performing molecular technique to conduct CCS and monogenic disease analysis...
July 16, 2016: Journal of Assisted Reproduction and Genetics
Demián Glujovsky, Cindy Farquhar, Andrea Marta Quinteiro Retamar, Cristian Roberto Alvarez Sedo, Deborah Blake
BACKGROUND: Advances in cell culture media have led to a shift in in vitro fertilisation (IVF) practice from cleavage stage embryo transfer to blastocyst stage transfer. The rationale for blastocyst transfer is to improve both uterine and embryonic synchronicity and enable self selection of viable embryos, thus resulting in better live birth rates. OBJECTIVES: To determine whether blastocyst stage (day 5 to 6) embryo transfers improve the live birth rate, and other associated outcomes, compared with cleavage stage (day 2 to 3) embryo transfers...
2016: Cochrane Database of Systematic Reviews
Julie Lamy, Philippe Liere, Antoine Pianos, Fanny Aprahamian, Pascal Mermillod, Marie Saint-Dizier
Ovarian steroid hormones are major regulators of the physiology of the oviduct and reproductive events occurring within the oviduct. To establish a whole steroid profiling of the bovine oviductal fluid (OF) during the estrous cycle, contralateral and ipsilateral (to the corpus luteum or preovulatory follicle) oviducts were classified into four stages of the estrous cycle (n = 18-27 cows per stage): postovulatory (Post-ov), mid-luteal (Mid-lut), late luteal (Late-lut), and preovulatory on the basis of the ovarian morphology and intrafollicular steroid concentrations...
October 1, 2016: Theriogenology
Michael R Strug, Renwei Su, James E Young, William G Dodds, Valerie I Shavell, Patricia Díaz-Gimeno, Maria Ruíz-Alonso, Carlos Simón, Bruce A Lessey, Richard E Leach, Asgerally T Fazleabas
STUDY QUESTION: Does a single intrauterine infusion of human chorionic gonadotropin (hCG) at the time corresponding to a Day 3 embryo transfer in oocyte donors induce favorable molecular changes in the endometrium for embryo implantation? SUMMARY ANSWER: Intrauterine hCG was associated with endometrial synchronization between endometrial glands and stroma following ovarian stimulation and the induction of early decidual markers associated with stromal cell survival...
July 2016: Human Reproduction
Irina A Tarasova, Anna A Lobas, Urh Černigoj, Elizaveta M Solovyeva, Barbara Mahlberg, Mark V Ivanov, Tanja Panić-Janković, Zoltan Nagy, Marina L Pridatchenko, Andras Pungor, Blaž Nemec, Urška Vidic, Jernej Gašperšič, Nika Lendero Krajnc, Jana Vidič, Mikhail V Gorshkov, Goran Mitulović
Affinity depletion of abundant proteins such as HSA is an important stage in routine sample preparation prior to MS/MS analysis of biological samples with high range of concentrations. Due to the charge competition effects in electrospray ion source that results in discrimination of the low-abundance species, as well as limited dynamic range of MS/MS, restricted typically by three orders of magnitude, the identification of low-abundance proteins becomes a challenge unless the sample is depleted from high-concentration compounds...
September 2016: Electrophoresis
Sander H M Kleijkers, Aafke P A van Montfoort, Otto Bekers, Edith Coonen, Josien G Derhaag, Johannes L H Evers, John C M Dumoulin
STUDY QUESTION: Does ammonium accumulate in commercially available culture media and protein supplements used for in vitro development of human pre-implantation embryos during storage and incubation? SUMMARY ANSWER: Ammonium accumulates in ready-to-use in vitro fertilization (IVF) culture media during storage at 2-8°C and in ready-to-use IVF culture media and protein supplements during incubation at 37°C. WHAT IS KNOWN ALREADY: Both animal and human studies have shown that the presence of ammonium in culture medium has detrimental effects on embryonic development and pregnancy rate...
June 2016: Human Reproduction
Navid Dadashpour Davachi, Ahmad Zare Shahneh, Hamid Kohram, Mahdi Zhandi, Helia Shamsi, Amir M Hajiyavand, Mozafar Saadat
The central role of the oviduct, as the site of zona pellucida (ZP) maturation, fertilization and early embryogenesis, has been recognized. The objective of this study was to investigate whether ampullary and isthmic derived epithelial cells have different effects on in vitro ZP hardening, in vitro fertilization (IVF) and in vitro culture (IVC) of the resulting embryos. Cumulus oocyte complexes (COCs) were matured in a coculture system with ampullary/isthmic epithelial cells, TCM199 supplemented with insulin-like growth factor I (IGF-I) and epithelial derived growth factor (EGF) (GF treated group), conditioned media produced using ampullary (ACM), isthmic (ICM), COCs+ampullary, and COCs+isthmic epithelial cells, contactless culture system, oviductal fluid, GF+ACM/ICM, and drops of TCM199 (control), for 24h...
March 2016: Reproductive Biology
Akiko Yagi, Satoshi Miyanaga, Rojeet Shrestha, Seiji Takeda, Seiichi Kobayashi, Hitoshi Chiba, Hirobumi Kamiya, Shu-Ping Hui
BACKGROUND: Liquid chromatography-high resolution mass spectrometry (LC-HRMS) can be useful to improve in vitro fertilization (IVF). This study aims to find out an association between embryonic growth and embryonic uptake of free fatty acid (FFA) from culture media by using LC-HRMS. METHODS: Embryos (n=55) from 15 couples undergoing IVF were studied. An embryo was cultivated for up to 6 days in a 20 μl-medium drop under mineral oil, and classified by a morphological grading system into the good-growth group (n=32; good quality blastocysts) and the poor-growth group (n=23; non-blastocysts)...
May 1, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
Piotr Marianowski, Filip A Dąbrowski, Aleksandra Zyguła, Mirosław Wielgoś, Iwona Szymusik
Adverse perinatal outcomes in singleton IVF pregnancies have been most often explained by parental underlying diseases and so far laboratory conditions during embryo culture are still not explored well. The following review discusses the current state of knowledge on the influence of IVF laboratory procedures on the possible perinatal outcome. The role of improved media for human embryo culture is unquestionable. Addition of certain components to culture media and their effect on embryo survival and implantation rates have been taken into consideration recently and studied on animal model...
2016: BioMed Research International
Jun Young Lee, Yun Gil Jung, Byoung Boo Seo
BACKGROUND: This study was investigated the effects of culture media conditions on production of eggs fertilized in vitro of embryos from ovaries of high grade Korean native cow, Hanwoo. METHODS: The IVMD 101 and IVF 100 were used for in vitro maturation of selected Hanwoo oocytes and In vitro embryo culture after in vitro fertilization, respectively. The IVMD 101 and IVD 101 were used for in vitro culture and completely free of serum. RESULTS: The cleavage rates of 2-cell embryos in reference to Hanwoo oocytes were 86...
2016: Journal of Animal Science and Technology
M Durand, N Sermondade, C Herbemont, J Benard, H Gronier, J Boujenah, I Cédrin-Durnerin, C Poncelet, M Grynberg, C Sifer
OBJECTIVE: The aim of this study was to compare embryo development cultured in two single-step media commercially available: Fert/Sage One Step® (Origio) and Continuous Single Culture® (CSC) (Irvine Scientific). METHODS: A prospective auto-controlled study of sibling oocytes from women undergoing conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) was performed in our center from February to June 2015. After fertilization, for every patient, half of oocytes were cultured in the single-step Fert/Sage One Step® (serie SAGE) and the other half in the single-step CSC®(serie CSC)...
March 2016: Gynécologie, Obstétrique & Fertilité
Marie D Werner, Kathleen H Hong, Jason M Franasiak, Eric J Forman, Christine V Reda, Thomas A Molinaro, Kathleen M Upham, Richard T Scott
OBJECTIVE: To determine whether sequential or monophasic media is the more optimal formulation for blastocyst development and sustained implantation rates (SIR) in IVF. DESIGN: Paired randomized controlled trials. SETTING: Academic. PATIENT(S): Infertile couples (N = 192) with female partner ≤42 years old and normal ovarian reserve. INTERVENTION(S): Fertilized zygotes from each patient were randomly divided into two groups: [1] cultured in sequential media and [2] cultured in monophasic medium...
May 2016: Fertility and Sterility
Ivan Bochev, Kalina Belemezova, Atanas Shterev, Stanimir Kyurkchiev
PURPOSE: Along with comparative investigation of the decidualization potential and IL-6 secretion by fresh and frozen ESCs, we also aimed to evaluate the effectiveness of co-culture systems based on fresh or frozen ESCs in terms of clinical pregnancy rates. METHODS: Outcome analysis of a total of 215 IVF cycles with co-culture with fresh or frozen ESCs was performed. Endometrial tissue was obtained from 17 healthy donors. Concentrations of secreted prolactin, IGFBP-1, and IL-6 in conditioned media from cultured fresh and frozen ESCs (decidualized or not) were measured using ELISA or ECLIA...
April 2016: Journal of Assisted Reproduction and Genetics
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