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https://www.readbyqxmd.com/read/28646915/isolation-speciation-and-antifungal-susceptibility-testing-of-candida-isolates-from-various-clinical-specimens-at-a-tertiary-care-hospital-nepal
#1
Sundar Khadka, Jeevan Bahadur Sherchand, Bharat Mani Pokhrel, Keshab Parajuli, Shyam Kumar Mishra, Sangita Sharma, Niranjan Shah, Hari Prasad Kattel, Subhash Dhital, Sulochana Khatiwada, Narayan Parajuli, Manoj Pradhan, Basista Prasad Rijal
BACKGROUND: Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases along with increased resistance to antifungal drugs has made a serious concern. Resistance to antifungal agents has increased during the last decade. Thus, identification of Candida up to species level and its antifungal susceptibility testing has a paramount significance in the management of Candidal infections. The aim of the study was to speciate Candida species and to determine antifungal susceptibility pattern of Candida species to antifungal agents...
June 24, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28644688/intraosseous-administration-of-antidotes-a-systematic-review
#2
Audrée Elliott, Pierre-André Dubé, Amélie Cossette-Côté, Laura Patakfalvi, Eric Villeneuve, Martin Morris, Sophie Gosselin
CONTEXT: Intraosseous (IO) access is an established route of administration in resuscitation situations. Patients with serious poisoning presenting to the emergency department may require urgent antidote therapy. However, intravenous (IV) access is not always readily available. OBJECTIVE: This study reviews the current evidence for IO administration of antidotes that could be used in poisoning. The primary outcome was mortality as a surrogate of efficacy. Secondary outcomes included hemodynamic variables, electrocardiographic variables, neurological status, pharmacokinetics outcomes, and adverse effects as defined by each article...
June 23, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28640833/investigation-of-pseudomonas-fluorescens-strain-3jw1-on-preventing-and-reducing-aflatoxin-contaminations-in-peanuts
#3
Xiaona Yang, Qi Zhang, Zhi-Yuan Chen, Hongxia Liu, Peiwu Li
Pseudomonas fluorescens strain 3JW1, which has a broad-spectrum antimicrobial activity, was studied to investigate whether it affects the amounts of aflatoxin B1 (AFB1) produced by Aspergillus flavus. It was found that the bacterium reduced the amounts of AFB1 in potato dextrose broth (PDB) and peanut medium by 97.8% and 99.4%, respectively. It also reduced AFB1 by ~183 μg/kg (55.8%) when applied onto peanut kernels. This strain reduced AFB1 via three mechanisms. First, it significantly inhibited A. flavus growth; second, our data showed that strain 3JW1 inhibits aflatoxin biosynthesis by A...
2017: PloS One
https://www.readbyqxmd.com/read/28640741/an-endophytic-fusarium-sp-isolated-from-monarda-citriodora-produces-the-industrially-important-plant-like-volatile-organic-compound-hexanal
#4
Meenu Katoch, Kushal Bindu, Shipra Phull, M K Verma
An endophytic fungus, MC_25L, has been isolated from the leaves of MonardacitriodoraCerv. ex Lag., a medicinal and aromatic herb from the northwestern Himalayas. It produces a fruity fragrance while growing on potato dextrose agar, suggesting that it is producing volatile organic compounds (VOCs). The endophyte inhibited the growth of plant pathogens such asSclerotiniasp. and Aspergillusflavus by virtue of VOCs. Identification of MC_25L based on morphological and microscopic features, as well as ITS-based rDNA sequence analysis, revealed that it is a Fusariumsp...
June 22, 2017: Microbiology
https://www.readbyqxmd.com/read/28638573/acute-intermittent-porphyria-a-test-of-clinical-acumen
#5
Rashmi Dhital, Sijan Basnet, Dilli Ram Poudel, Khema Raj Bhusal
Acute intermittent porphyria (AIP) is a rare autosomal dominant hepatic porphyria due to deficiency of hydroxymethylbilane synthase (HMBS), also known as porphobilinogen deaminase leading to accumulation of porphyrin precursors. However, gene defect alone is usually not sufficient to cause an acute attack, and many extrinsic factors play a role. Diagnostic tests are defined, but clinical suspicion is often delayed as symptoms mimic other common conditions. We report a case of a 18-year-old male with severe, persistent, and generalized abdominal pain along with marked hyponatremia, with subsequent development of altered mentation needing intensive care...
March 2017: Journal of Community Hospital Internal Medicine Perspectives
https://www.readbyqxmd.com/read/28638159/in-vitro-evaluation-of-the-fresenius-kabi-catsmart-autotransfusion-system
#6
Melissa Alberts, Robert C Groom, Richard Walczak, Robert Kramer, Adrienne Karpiel, Jeanette Dieter, Lisa Sheth, Nathaniel H Greene, Edmund H Jooste
Use of autotransfusion systems to collect, wash, and concentrate shed blood during surgical procedures is a widely used method for reducing postoperative anemia and the need for blood transfusions. The aim of this study was to evaluate the CATSmart Continuous Autotransfusion System wash program performance with small (200 or 700 mL) and large volumes (1,000 mL) of shed blood and to determine non-inferiority of the CATSmart to the C.A.T.S (plus) system. Human whole blood was collected in citrate phosphate dextrose, diluted, and divided into two aliquots to be processed as a pair using the C...
June 2017: Journal of Extra-corporeal Technology
https://www.readbyqxmd.com/read/28637358/comparative-simulation-study-of-glucose-control-methods-designed-for-use-in-the-intensive-care-unit-setting-via-a-novel-controller-scoring-metric
#7
Jeremy DeJournett, Leon DeJournett
BACKGROUND: Effective glucose control in the intensive care unit (ICU) setting has the potential to decrease morbidity and mortality rates and thereby decrease health care expenditures. To evaluate what constitutes effective glucose control, typically several metrics are reported, including time in range, time in mild and severe hypoglycemia, coefficient of variation, and others. To date, there is no one metric that combines all of these individual metrics to give a number indicative of overall performance...
June 1, 2017: Journal of Diabetes Science and Technology
https://www.readbyqxmd.com/read/28636663/an-endophytic-basidiomycete-grammothele-lineata-isolated-from-corchorus-olitorius-produces-paclitaxel-that-shows-cytotoxicity
#8
Avizit Das, Mohammad Imtiazur Rahman, Ahlan Sabah Ferdous, Al- Amin, Mohammad Mahbubur Rahman, Nilufar Nahar, Md Aftab Uddin, Mohammad Riazul Islam, Haseena Khan
Grammothele lineata, an endophyte isolated in our laboratory from jute (Corchorus olitorius acc. 2015) was found to be a substantial paclitaxel producer. Taxol and its related compounds, produced by this endophyte were extracted by growing the fungus in simple nutrient media (potato dextrose broth, PDB). Taxol was identified and characterized by different analytical techniques (TLC, HPLC, FTIR, LC-ESI-MS/MS) following its extraction by ethyl acetate. In PDB media, this fungus was found to produce 382.2 μgL-1 of taxol which is about 7...
2017: PloS One
https://www.readbyqxmd.com/read/28630182/study-of-biodistribution-and-in-vivo-anti-leishmanial-activity-of-a-distigmasterylhemisuccinoyl-glycero-phosphocholine-liposome-intercalated-amphotericin-b
#9
Maryam Iman, Zhaohua Huang, Seyedeh Hoda Alavizadeh, Francis C Szoka, Mahmoud R Jaafari
Distigmasterylhemisuccinoyl-sn-glycero-3- phosphocholine (DSHemsPC) is a new lipid in which two molecules of stigmasterol (an inexpensive plant sterol) are covalently linked via a succinic acid to glycerophosphocholine. Our previous study revealed that a liposome-intercalated Amphotericin B prepared from DSHemsPC (DSHemsPC-Lip-AmB) possess excellent colloidal properties and in vitro antifungal and anti-leishmanial activity similar to AmBisome™. The aim of this study was to determine biodistribution and evaluate the anti-leishmanial effects of DSHemsPC-Lip-AmB in Leishmania major-infected BALB/c mice...
June 19, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28628923/cremophor-el-alters-the-plasma-protein-binding-and-pharmacokinetic-profile-of-valspodar-in-rats
#10
Ziyad Binkhathlan
Cremophor EL is a nonionic surfactant widely used in pharmaceutical formulations. Nonetheless, there are several reports on the influence of this excipient on the protein binding, pharmacokinetics, and pharmacodynamics of drugs. Valspodar is an investigational non-immunosuppressive derivative of cyclosporine A, used in clinical trials for treatment of multidrug resistant tumors. The formulation of valspodar (Amdray(®)) contains cremophor EL and ethanol as solubilizing agents. The main aim of the current study was to assess the plasma protein binding (in vitro) and the pharmacokinetic profile of valspodar in the cremophor EL-based formulation in comparison to a cremophor EL-free formulation following intravenous (i...
June 19, 2017: Drug Research
https://www.readbyqxmd.com/read/28625451/is-perioperative-administration-of-5-dextrose-effective-in-reducing-the-incidence-of-ponv-in-laparoscopic-cholecystectomy-a-randomized-control-trial
#11
Ankita Mishra, Ravinder Kumar Pandey, Ankur Sharma, Vanlalnghaka Darlong, Jyotsna Punj, Devalina Goswami, Renu Sinha, Vimi Rewari, Chandralekha Chandralekha, Virinder Kumar Bansal
STUDY OBJECTIVE: To compare the incidence of postoperative nausea and vomiting (PONV) during perioperative administration of 5% dextrose and normal saline in laparoscopic cholecystectomy. DESIGN: Prospective, randomized, double-blind trial. SETTING: Operating rooms in a tertiary care hospital of Northern India. PATIENTS: One hundred patients with American Society of Anesthesiologists status I to II undergoing laparoscopic cholecystectomy were enrolled in this study...
August 2017: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/28622549/immunological-and-epidemiological-factors-affecting-candidiasis-in-hiv-patients-beginning-antiretroviral-therapy-in-an-asian-clinic
#12
Endah A T Wulandari, Henny Saraswati, Robiatul Adawiyah, Samsuridjal Djauzi, Retno Wahyuningsih, Patricia Price
OBJECTIVES: Oropharyngeal candidiasis (OPC) is common in HIV patients beginning antiretroviral therapy (ART). Here we address the response to ART, and the roles of poor oral hygiene and defects in local innate immunity with a focus on salivary β-defensins, as they are implicated in control of candidiasis but have not been investigated in this context. DESIGN: ART naïve HIV-infected adults (n=82) with <200 CD4(+) T-cells/mm(3) attending clinics at Cipto Mangunkusumo Hospital, Jakarta, were examined at the commencement of ART, and 73 were re-examined after 3 months...
June 3, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28620360/cytr-homolog-of-pectobacterium-carotovorum-subsp-carotovorum-controls-air-liquid-biofilm-formation-by-regulating-multiple-genes-involved-in-cellulose-production-c-di-gmp-signaling-motility-and-type-iii-secretion-system-in-response-to-nutritional-and-environmental
#13
M M Haque, M M H Oliver, Kamrun Nahar, Mohammad Z Alam, Hisae Hirata, Shinji Tsuyumu
Pectobacterium carotovorum subsp. carotovorum [Pcc (formerly Erwinia carotovora subsp. carotovora)] PC1 causes soft-rot disease in a wide variety of plant species by secreting multiple pathogenicity-related traits. In this study, regulatory mechanism of air-liquid (AL) biofilm formation was studied using a cytR homolog gene deletion mutant (ΔcytR) of Pcc PC1. Compared to the wild type (Pcc PC1), the ΔcytR mutant produced fragile and significantly (P < 0.001) lower amounts of AL biofilm on salt-optimized broth plus 2% glycerol (SOBG), yeast peptone dextrose adenine, and also on King's B at 27°C after 72 h incubation in static condition...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28619742/postgastric-bypass-hypoglycaemia-in-a-patient-with-end-stage-renal-disease-a-diagnostic-and-management-pitfall
#14
Sameen Khalid, Anika Bilal, F N U Asad-Ur-Rahman, Richard Pratley
Roux-en-Y gastric bypass (RYGB) surgery is currently one of the most popular procedures to aid weight loss. Hypoglycaemia associated with gastric bypass surgery is an underdiagnosed but life-threatening potential consequence of the surgical procedure. We present a case of a 44-year-old woman with end-stage renal disease presenting with refractory hypoglycaemia after 10 years of RYGB. Extensive history and work-up excluded medications, renal disease, insulinoma and dumping syndrome as the cause of hypoglycaemia...
June 15, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28618654/comparison-of-honey-and-dextrose-solution-on-post-operative-peritoneal-adhesion-in-rat-model
#15
Vafa Baradaran Rahimi, Reza Shirazinia, Narges Fereydouni, Parvin Zamani, Sousan Darroudi, Amir Hossein Sahebkar, Vahid Reza Askari
BACKGROUND: Peritoneal adhesion between abdominal organs is a complication of surgery. It causes major complications like pain, bowel obstruction, infertility and increases risk of death. Honey is known to have anti-inflammatory and antioxidant properties potentially relevant for adhesive protection. METHODS: Thirty rats were divided into five groups: negative control without any surgical procedure (normal group), control group treated with normal saline, experimental group I treated with 1ml of 10% honey, experimental group II treated with honey at half concentration of group I (honey0...
June 10, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28615883/clinicobiochemical-changes-in-ivermectin-toxicity-in-doberman-pinscher-pups
#16
Sahadeb Dey, Nitin P Kurade, Kishore Lal Khurana, Ananya Dan
This study is based on naturally intoxicated Doberman pinscher pups. Six Doberman pinscher pups were overdosed 50 times of recommended ivermectin dose by the pet owner and presented to Referral Veterinary Polyclinic section of this institute in a state of coma. The pups were unable to hold their head up and had no pupillary reflex. The pups were examined clinically and samples of blood were taken for haemato-biochemical studies, before and after treatment. During intoxication there was marked haemoconcentration, increase in values of serum creatinine phosphokinase, gamma glutamyl transpeptidase but decrease in serum glucose levels...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28614606/antagonism-of-trichoderma-isolates-against-leucoagaricus-gongylophorus-singer-m%C3%A3-ller
#17
Mariela Otoni do Nascimento, Renato de Almeida Sarmento, Gil Rodrigues Dos Santos, Cléia Almeida de Oliveira, Danival José de Souza
Filamentous fungi from the genus Trichoderma are commonly found in soil. They are considered facultative mycoparasites, and are antagonists of other fungi such as the cultivar of leaf-cutting ants (Leucoagaricus gongylophorus). The aim of the present study was to bioprospect Trichoderma spp. from different soils collected from Gurupi, Tocantins, Brazil, for antagonistic effects against the mutualistic fungus of leaf-cutting ants. To isolate filamentous fungi, samples were collected from six locations. Preliminarily, isolates were identified by morphological analysis as belonging to Trichoderma...
June 14, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28612562/-pancreatic-%C3%AE-cell-dysfunction-and-apoptosis-induced-by-elevated-free-fatty-acids-synergize-with-hyperglycemia
#18
Hui-Wen Tan, Nai-Qian Zhao, Ye-Rong Yu, Li-Na Han, Xiang-Xun Zhang
OBJECTIVES: To analysis the effects of glucoxicity and lipotoxicity on the function and apoptosis of pancreatic β-cells. METHODS: The levels of circulating glucose and free fat acids (FFAs) were elevated by infusion dextrose and fat emulsion in high-fat obese rats. The insulin resistance model obese rats were divided into four gourp: obese group with saline infusion (OB-NS group, n=7), obese group with glucose infusion (OB-GS group, n=9), obese group with Lipid emulsion infusion (OB-FFA group, n=8), obese group with glucose and lipid emulsion infusion (OB-FG group, n=9)...
January 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28606521/enzyme-resistant-dextrins-from-potato-starch-for-potential-application-in-the-beverage-industry
#19
Kamila Kapusniak Jochym, Ewa Nebesny
The objective of this study was to produce soluble enzyme-resistant dextrins by microwave heating of potato starch acidified with small amounts of hydrochloric and citric acids and to characterize their properties. Twenty five samples were initially made and their solubility was determined. Three samples with the highest water solubility were selected for physico-chemical (dextrose equivalent, molecular weight distribution, pasting characteristics, retrogradation tendency), total dietary fiber (TDF) analysis, and stability tests...
September 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28605322/antioxidant-and-cholesterol-esterase-inhibitory-properties-of-supplementation-with-coconut-water-in-submerged-cultivation-of-the-medicinal-chinese-caterpillar-mushroom-ophiocordyceps-sinensis-cs1197-ascomycetes
#20
G M Shashidhar, S Sravan Kumar, P Giridhar, B Manohar
We investigated the potential use of coconut water to supplement potato dextrose broth (PDB) in the production of Ophiocordyceps sinensis CS1197 by submerged cultivation. The basal PDB medium was modified by supplementation with tender coconut water (TCW) and mature coconut water (MCW) at 10% and 5% (v/v), respectively; these mixtures were cultured at 28°C for 14 days, with a pH of 7 and an inoculum volume of 10%. The addition of optimized levels of TCW and MCW improved the biomass yield by 2.2- and 2.5-fold, respectively, and adenosine, cordycepin, and polysaccharide content by 58% and 69%, 50% and 55%, and 19% and 27%, respectively...
2017: International Journal of Medicinal Mushrooms
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