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https://www.readbyqxmd.com/read/28430142/initial-spore-density-has-an-influence-on-ochratoxin-a-content-in-aspergillus-ochraceus-cgmcc-3-4412-in-pdb-and-its-interaction-with-seeds
#1
Caiyan Li, Yanmin Song, Lu Xiong, Kunlun Huang, Zhihong Liang
The morphology and secondary metabolism of Aspergillus spp. are associated with initial spore density (ISD). Fatty acids (FA) are involved in the biosynthesis of aflatoxins (AF) through Aspergillus quorum sensing (QS). Here, we studied how ochratoxin A (OTA) was regulated by spore density in Aspergillus ochraceus CGMCC 3.4412. The results contribute to understanding the role of spore density in morphogenesis, OTA biosynthesis, and host-pathogen interactions. When A. ochraceus was grown in Potato Dextrose Broth (PDB) media at different spore densities (from 10¹ to 10⁶ spores/mL), more OTA was produced when ISD were increased, but a higher level of ISD inhibited OTA biosynthesis...
April 21, 2017: Toxins
https://www.readbyqxmd.com/read/28421050/reveromycins-a-and-b-from-streptomyces-sp-3-10-antifungal-activity-against-plant-pathogenic-fungi-in-vitro-and-in-a-strawberry-food-model-system
#2
Ang Lyu, Hao Liu, Hongjie Che, Long Yang, Jing Zhang, Mingde Wu, Weidong Chen, Guoqing Li
This study was conducted to determine the antifungal activity of the metabolites from Streptomyces sp. 3-10, and to purify and identify the metabolites. Meanwhile, the taxonomic status of strain 3-10 was re-evaluated. The cultural filtrates of strain 3-10 in potato dextrose broth were extracted with ethyl acetate. The resulting crude extract at 1 and 5 μg/ml inhibited growth of 22 species in 18 genera of plant pathogenic fungi and Oomycetes, accounting for 92% of the total 24 tested species, suggesting that it has a wide antifungal spectrum...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28420095/parenteral-nutrition-and-lipids
#3
REVIEW
Maitreyi Raman, Abdulelah Almutairdi, Leanne Mulesa, Cathy Alberda, Colleen Beattie, Leah Gramlich
Lipids have multiple physiological roles that are biologically vital. Soybean oil lipid emulsions have been the mainstay of parenteral nutrition lipid formulations for decades in North America. Utilizing intravenous lipid emulsions in parenteral nutrition has minimized the dependence on dextrose as a major source of nonprotein calories and prevents the clinical consequences of essential fatty acid deficiency. Emerging literature has indicated that there are benefits to utilizing alternative lipids such as olive/soy-based formulations, and combination lipids such as soy/MCT/olive/fish oil, compared with soybean based lipids, as they have less inflammatory properties, are immune modulating, have higher antioxidant content, decrease risk of cholestasis, and improve clinical outcomes in certain subgroups of patients...
April 14, 2017: Nutrients
https://www.readbyqxmd.com/read/28416347/blood-collection-vials-and-clinically-used-intravenous-fluids-contain-significant-amounts-of-nitrite
#4
Luis E F Almeida, Sayuri Kamimura, Margaret Y Nettleton, Celia M de Souza Batista, Elizabeth Walek, Alfia Khaibullina, Li Wang, Zenaide M N Quezado
The biology of the inorganic anion nitrite is linked to nitric oxide (NO) as nitrite can be reduced to NO and mediate its biological activities. Thus, studies of nitrite biology require sensitive and selective chemical assays. The acetic and ascorbic acids method is selective for nitrite and measures it in biological matrices. However, one of the pitfalls of nitrite measurements is its ubiquitous presence in sample collection tubes. Here, we showed high levels of nitrite in collection tubes containing EDTA, sodium citrate or sodium heparin and smaller amounts in tubes containing lithium heparin or serum clot activator...
April 14, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28415387/dextrose-modified-flexible-tasar-and-muga-fibroin-films-for-wound-healing-applications
#5
Chandra Mohan Srivastava, Roli Purwar, Anuradha Gupta, Deepak Sharma
This paper is focused on preparation and characterization of regenerated muga and tasar fibroin flexible films from cocoon using ionic liquid. These flexible muga and tasar fibroin films were prepared by incorporating dextrose (5 to 15% w/w) as plasticizer. The mechanical, thermal, physical, morphological and biological properties of dextrose plasticized muga and tasar fibroin films were characterized. These plasticized films showed higher elongation at break as well as water holding capacity as compared to the un-plasticized films...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28414109/synthesis-and-characterization-of-chitosan-nanoparticles-and-chitosan-effect-on-fusarium-head-blight-and-oxidative-activity-in-wheat
#6
A Kheiri, S A Moosawi Jorf, A Malihipour, H Saremi, M Nikkhah
The main aim of present study was to prepare chitosan (CS) and chitosan nanoparticles (CS/NPs) to evaluate their antifungal and oxidative activity. CS/NPs were prepared based on the ionic gelation of CS with tripolyphosphate (TPP) anions by using centrifugation and pH change. The obtained nanoparticles (NPs) were characterized by size and zeta potential analysis. The antifungal activity of the CS and CS/NPs were evaluated on the Fusarium graminearum, which causes Fusarium head blight (FHB) on wheat by the method of spraying on the Potato dextrose agar (PDA) medium...
April 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28411369/comparison-of-equine-platelet-function-and-survival-in-whole-blood-collected-in-acid-citrate-dextrose-solution-or-citrate-phosphate-dextrose-adenine-solution
#7
Rana Bozorgmanesh, Julie W Sutton-Burges, Fern Tablin
BACKGROUND: Equine whole blood collection and storage methods have been evaluated to assess red blood cell viability; however, platelet (PLT) viability has not been comprehensively assessed. OBJECTIVES: The purpose of the study was to compare viability of PLTs collected in whole blood into 2 different anticoagulants. METHODS: Whole blood from 6 healthy adult Thoroughbred horses was collected into citrate-phosphate-dextrose-adenine (CPDA) or acid-citrate-dextrose (ACD)...
April 14, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28401111/added-sugar-in-the-packaged-foods-and-beverages-available-at-a-major-canadian-retailer-in-2015-a-descriptive-analysis
#8
Rachel B Acton, Lana Vanderlee, Erin P Hobin, David Hammond
BACKGROUND: Excess consumption of added sugars has been associated with a variety of health problems, but there is little information available characterizing added sugar in the Canadian food supply. This study examined the presence and types of added sugars in the packaged food and beverage products available at a major Canadian grocery retailer. METHODS: We searched the ingredients lists of over 40 000 packaged food products available for sale in March 2015 for a variety of added sugar terms...
January 2017: CMAJ Open
https://www.readbyqxmd.com/read/28399217/fungi-isolated-from-house-flies-diptera-muscidae-on-penned-cattle-in-south-texas
#9
Cherity A Ysquierdo, Pia U Olafson, Donald B Thomas
Musca domestica L. were collected from cattle diagnosed with bovine ringworm to evaluate the potential of the house fly to disseminate Trichophyton verrucosum E. Bodin, a fungal dermatophyte that is the causative agent for ringworm in cattle. Fungal isolates were cultured from 45 individual flies on supplemented Sabouraud dextrose agar, and isolates were identified using morphological and microscopic approaches. Each isolate was identified further by PCR amplification of the ribosomal DNA locus with fungal-specific primers and subsequent amplicon sequencing...
February 4, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28392692/antifungal-optical-and-mechanical-properties-of-polymethylmethacrylate-material-incorporated-with-silanized-zinc-oxide-nanoparticles
#10
Krid Kamonkhantikul, Mansuang Arksornnukit, Hidekazu Takahashi
BACKGROUND: Fungal infected denture, which is typically composed of polymethylmethacrylate (PMMA), is a common problem for a denture wearer, especially an elderly patient with limited manual dexterity. Therefore, increasing the antifungal effect of denture by incorporating surface modification nanoparticles into the PMMA, while retaining its mechanical properties, is of interest. AIM OF THE STUDY: This study aimed to evaluate antifungal, optical, and mechanical properties of heat-cured PMMA incorporated with different amounts of zinc oxide nanoparticles (ZnOnps) with or without methacryloxypropyltrimethoxysilane modification...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28391562/the-ability-of-brown-rot-fungus-daedalea-dickinsii-to-decolorize-and-transform-methylene-blue-dye
#11
Hamdan Dwi Rizqi, Adi Setyo Purnomo
The ability of Daedalea dickinsii to decolorize and transform methylene blue (MB) dye was investigated. MB was decolorized in potato dextrose agar medium after adding MB at concentrations of 50, 75, and 100 mg L(-1). D. dickinsii decolorized MB with decolorization index values of 0.92, 0.90, and 0.88 at MB concentrations of 50, 75, and 100 mg L(-1), respectively. The 100 mg L(1) MB concentration was selected for biotransformation in liquid potato dextrose broth medium. D. dickinsii transformed approximately 54% of the MB after a 14-day incubation...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28387754/erratum-hypertonic-dextrose-injections-prolotherapy-in-the-treatment-of-symptomatic-knee-osteoarthritis-a-systematic-review-and-meta-analysis
#12
Regina Ws Sit, Vincent Ch Chung, Kenneth D Reeves, David Rabago, Keith Kw Chan, Dicken Cc Chan, Xinyin Wu, Robin St Ho, Samuel Ys Wong
No abstract text is available yet for this article.
April 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28384860/fungal-opportunistic-pneumonias-in-hiv-aids-patients-an-indian-tertiary-care-experience
#13
Ravinder Kaur, Bhanu Mehra, Megh Singh Dhakad, Ritu Goyal, Preena Bhalla, Richa Dewan
INTRODUCTION: Opportunistic pneumonias are a major cause of mortality and morbidity in Human Immunodeficiency Virus (HIV) reactive patients. Despite the significant role that fungi play in causation of this opportunistic mycoses, very few Indian studies have attempted to investigate the burden and aetiological spectrum of HIV/AIDS-associated fungal pneumonias. AIM: To document the prevalence of fungal aetiology in HIV/AIDS-related opportunistic pneumonias in an Indian setting; and to elucidate the various fungal opportunists responsible for the same...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28377939/endoscopic-treatment-of-bleeding-diversion-pouchitis-with-high-concentration-dextrose-spray
#14
Custon T Nyabanga, Bo Shen
Surgical closure of stoma with the reestablishment of gut continuity is the only curative intervention available for inflammatory bowel disease patients with diversion pouchitis, proctitis, or colitis. For patients who are not candidates for surgical reestablishment of bowel continuity, the alternative nonsurgical approaches, such as topical therapy with mesalamine, corticosteroids, or short-chain fatty acids, have only shown modest efficacy. The management of massive bleeding from diversion pouchitis has not been described...
2017: ACG Case Reports Journal
https://www.readbyqxmd.com/read/28377317/dehydroascorbate-derivatized-chitosan-particles-for-targeting-antimalarial-agents-to-infected-erythrocytes
#15
Hasham Shafi, D V Siva Reddy, Tabassum Khan, Rajeev Ranjan, Ashish Srivastava, Suniti Vaishya, Tanuj Sharma, Mohammad Imran Siddiqui, Saman Habib, Amit Misra
The mammalian glucose transporter GLUT-1 and Plasmodium falciparum hexose transporter PfHT1 are overexpressed on human RBC infected with the parasite (iRBC), presumably for enhanced glucose uptake. Dehydroascorbic acid (DHA) competes out glucose in GLUT-1 binding. We prepared particles containing chloroquine phosphate using novel derivatives of chitosan (CSN). CSN was either pre-derivatized with DHA (PRE) or particles made of CSN were derivatized by surface-grafting DHA (POST). The optimized formulations were analyzed for size (170-200nm) drug content (about 40%) entrapment efficiency (50-57%), in vitro drug release (80% in 72h, Higuchi's model), hemolysis on exposure to whole blood or RBC at 5% hematocrit, cytotoxicity towards cultured HEK 293T (kidney) and HepG2 (hepatic) cells, targeting iRBC and in vitro efficacy against P...
April 2, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28376610/purification-and-characterization-of-the-laccase-involved-in-dye-decolorization-by-the-white-rot-fungus-marasmius-scorodonius
#16
Sung-Jong Jeon, Su-Jin Lim
Marasmius scorodonius secretes an extracellular laccase in potato dextrose broth, and this enzyme was purified up to 206-fold using (NH4)2SO4 precipitation and a Hi-trap Q Sepharose column. The molecular weight of the purified laccase was estimated to be ~67 kDa by SDS-PAGE. The UV/vis spectrum of the enzyme is nontypical for laccases, and metal content analysis revealed that the enzyme contains 1 mole of Fe and Zn and 2 moles of Cu per mole of protein. The optimal pH for the enzymatic activity was 3.4, 4.0 and 4...
April 5, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28373501/synthetic-dextrose-proline-sdp-plates-with-drugs
#17
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373500/synthetic-dextrose-glutamate-sde-plates-with-drugs
#18
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373499/synthetic-dextrose-sd-plates-without-ammonium-sulfate-or-amino-acids-with-or-without-acetamide-or-cytosine
#19
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373498/synthetic-dextrose-sd-plates-with-or-without-cytosine
#20
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
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