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https://www.readbyqxmd.com/read/28531168/a-novel-millet-based-probiotic-fermented-food-for-the-developing-world
#1
Elisa Di Stefano, Jessica White, Shannon Seney, Sharareh Hekmat, Tim McDowell, Mark Sumarah, Gregor Reid
Probiotic yogurt, comprised of a Fiti sachet containing Lactobacillus rhamnosus GR-1 and Streptococcus thermophilus C106, has been used in the developing world, notably Africa, to alleviate malnutrition and disease. In sub-Saharan African countries, fermentation of cereals such as millet, is culturally significant. The aim of this study was to investigate the fermentation capability of millet when one gram of the Fiti sachet consortium was added. An increase of 1.8 and 1.4 log CFU/mL was observed for S. thermophilus C106 and L...
May 22, 2017: Nutrients
https://www.readbyqxmd.com/read/28523031/myocardial-protection-with-glucose-insulin-potassium-infusion-during-adult-cardiac-surgery
#2
Suhail Ahmad, Rana Altaf Ahmad, Bilal Ahsan Qureshi, Mirza Ahmad Raza Baig
BACKGROUND & OBJECTIVE: Recent meta-analysis reports have called for more randomized trials to evaluate the effectiveness of GIK solution in patients of cardiac surgery. So this study was conducted to evaluate the effectiveness of Glucose-insulin-potassium (GIK) solutions in non-diabetic patients undergoing coronary artery bypass grafting. METHODS: A total number of one hundred and sixty (160) patients were randomized into two equal groups; GIK Group and non-GIK group...
March 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/28518051/red-blood-cells-ageing-markers-a-multi-parametric-analysis
#3
Manon Bardyn, Benjamin Rappaz, Keyvan Jaferzadeh, David Crettaz, Jean-Daniel Tissot, Inkyu Moon, Gerardo Turcatti, Niels Lion, Michel Prudent
BACKGROUND: Red blood cells collected in citrate-phosphate-dextrose can be stored for up to 42 days at 4 °C in saline-adenine-glucose-mannitol additive solution. During this controlled, but nevertheless artificial, ex vivo ageing, red blood cells accumulate lesions that can be reversible or irreversible upon transfusion. The aim of the present study is to follow several parameters reflecting cell metabolism, antioxidant defences, morphology and membrane dynamics during storage. MATERIALS AND METHODS: Five erythrocyte concentrates were followed weekly during 71 days...
May 2017: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/28512455/non-edible-oil-cakes-as-a-novel-substrate-for-dpa-production-and-augmenting-biocontrol-activity-of-paecilomyces-variotii
#4
Kalpana Arora, Satyawati Sharma, Suresh B N Krishna, Jamila K Adam, Ashwani Kumar
The present study investigated the use of waste non-edible oil cakes (Jatropha, Karanja, Neem, and Mahua) as a substrate for the growth of Paecilomyces variotii and dipicolinic acid (DPA) production. Previous researches proved the efficacy of DPA in suppressing certain pathogens that are deleterious to the plants in the rhizosphere. DPA production was statistical optimized by amending non-edible oil cakes in growing media as nitrogen and sugars (Dextrose, Glucose, and Lactose) as carbon source. Plackett-Burman design (PBD), indicated that Jatropha cake, Karanja cake, and Dextrose were the most significant components (p < 0...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28511380/fungal-profile-of-vulvovaginal-candidiasis-in-a-tertiary-care-hospital
#5
Krishnapriya Kalaiarasan, Rakesh Singh, Latha Chaturvedula
INTRODUCTION: Vulvovaginal Candidiasis (VVC) is a common medical health problem of adult women. It is most commonly caused by Candida albicans. But there is a change in fungal profile. Sabouraud's Dextrose Agar (SDA) is the most common culture medium used where mixed fungal infection may be missed. It can be detected easily by using chromogenic culture medium. AIM: To know the fungal profile of vulvovaginal candidiasis using Candida CHROMagar and antifungal susceptibility pattern in patients attending tertiary care hospital...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28511379/malassezia-yeast-and-cytokine-gene-polymorphism-in-atopic-dermatitis
#6
Charu Jain, Shukla Das, V G Ramachandran, Rumpa Saha, S N Bhattacharya, Sajad Dar
INTRODUCTION: Atopic Dermatitis (AD) is a recurrent chronic condition associated with microorganism and their interaction with the susceptible host. Malassezia yeast is a known commensal which is thought to provoke the recurrent episodes of symptoms in atopic dermatitis patients. Malassezia immunomodulatory properties along with defective skin barrier in such host, results in disease manifestation. Here, we studied Single Nucleotide Polymorphism (SNP) in IL10 and IFN γ genes of the host and its relation with susceptibility to Malassezia infection...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28498319/ctc-mrna-ar-v7-analysis-from-blood-samples-impact-of-blood-collection-tube-and-storage-time
#7
Alison W S Luk, Yafeng Ma, Pei N Ding, Francis P Young, Wei Chua, Bavanthi Balakrishnar, Daniel T Dransfield, Paul de Souza, Therese M Becker
Circulating tumour cells (CTCs) are an emerging resource for monitoring cancer biomarkers. New technologies for CTC isolation and biomarker detection are increasingly sensitive, however, the ideal blood storage conditions to preserve CTC-specific mRNA biomarkers remains undetermined. Here we tested the preservation of tumour cells and CTC-mRNA over time in common anticoagulant ethylene-diamine-tetra-acetic acid (EDTA) and acid citrate dextrose solution B (Citrate) blood tubes compared to preservative-containing blood tubes...
May 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28495827/hyperbaric-oxygen-reduces-infarction-volume-and-hemorrhagic-transformation-through-atp-nad-sirt1-pathway-in-hyperglycemic-middle-cerebral-artery-occlusion-rats
#8
Qin Hu, Anatol Manaenko, Hetao Bian, Zongduo Guo, Jun-Long Huang, Zhen-Ni Guo, Peng Yang, Jiping Tang, John H Zhang
BACKGROUND AND PURPOSE: Energy depletion is a critical factor leading to cell death and brain dysfunction after ischemic stroke. In this study, we investigated whether energy depletion is involved in hyperglycemia-induced hemorrhagic transformation after ischemic stroke and determined the pathway underlying the beneficial effects of hyperbaric oxygen (HBO). METHODS: After 2-hour middle cerebral artery occlusion, hyperglycemia was induced by injecting 50% dextrose (6 mL/kg) intraperitoneally at the onset of reperfusion...
May 11, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28494649/herbicidal-activity-of-pure-compound-isolated-from-rhizosphere-inhabiting-aspergillus-flavus
#9
Saeed Ullah Khattak, Ghosia Lutfullah, Zafar Iqbal, Irshad Ur Rehman, Jamshaid Ahmad, Abid Ali Khan
In the quest for bioactive natural products of fungal origin, Aspergillus flavus was isolated from rhizosphere of Mentha piperita using Potato Dextrose Agar (PDA) and Czapec Yeast Broth (CYB) nutrient media for metabolites production. In total, three different metabolites were purified using HPLC/LCMS and the structures were established using 500 Varian NMR experiments. Further the isolated metabolites in different concentrations (10, 100, 1000 μg/mL) were tested for herbicidal activity using Completely Randomized design (CRD) against the seeds of Silybum marianum and Avena fatua which are major threats to wheat crop in Pakistan...
May 11, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28483968/pre-analytical-stability-of-antibodies-to-pathogenic-antigens
#10
Verity S Hodgkinson, Sam Egger, Fay Betsou, Tim Waterboer, Michael Pawlita, Angelika Michel, Mark S Baker, Emily Banks, Freddy Sitas
BACKGROUND: Serological testing for antibodies against epitopes from pathogens is a valuable tool for investigating the relationship between infection and disease. This study comprehensively evaluates the impact of pre-analytic variation on antibody seropositivities to a selected set of antigens arising from delays in processing of blood samples, pre-processing storage-temperature and vacutainer-type. METHODS: We assessed peripheral blood collected from 29 volunteers in four different Vacutainer® types (ethylenediaminoetetraacetic-acid (EDTA), acid-citrate-dextrose (ACD), lithium heparin (LH) and serum separator tubes (SST)) and stored at 4◦C or room-temperature for 0, 1, 2, 3, 4, 5 and 6 days before processing...
May 8, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/28479018/levosimendan-mitigates-coagulopathy-and-organ-dysfunction-in-rats-with-endotoxemia
#11
Hsieh-Chou Huang, Hsin-Jung Tsai, Chao-Chun Wang, Cheng-Ming Tsao, Shuk-Man Ka, Wen-Jinn Liaw, Chin-Chen Wu
BACKGROUND: In patients with severe sepsis, pro-inflammatory cytokines and subsequent activation of tissue factors trigger a cascade of events that lead to coagulation dysfunction and multiple organ failure. It has been shown that levosimendan has protective effects against tissue injury caused by endotoxin. The purpose of this study was to evaluate the effects of levosimendan on consumptive coagulopathy and organ dysfunction in an endotoxemic animal model induced by lipopolysaccharide (LPS)...
May 3, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28477413/emergency-treatment-of-hypoglycaemia-a-guideline-and-evidence-review
#12
REVIEW
M Villani, B de Courten, S Zoungas
AIM: To examine the current treatment guidelines for the emergency management of hypoglycaemia and the evidence underpinning recommendations. METHODS: International diabetes agencies were searched for hypoglycaemia treatment guidelines. Guidelines were assessed using the Appraisal of Guidelines Research and Evaluation II (AGREE II) instrument. An electronic database search was conducted for evidence regarding emergency treatment of hypoglycaemia in adults, and relevant articles were critically appraised...
May 6, 2017: Diabetic Medicine: a Journal of the British Diabetic Association
https://www.readbyqxmd.com/read/28474424/-1-h-nmr-study-of-the-phase-behavior-of-aqueous-eutectic-solutions-using-the-inverse-laplace-transform-analysis-of-t2-relaxation
#13
Jessi A Baughman, G Joseph Ray, Christina M Szabo, Jane O Werling, Peter L Rinaldi
High-field (1) H NMR T2 relaxation studies were used to characterize the changes in the physical phases of water, NaCl, and dextrose solutions over a temperature range of -65 to 15 °C. The data were analyzed with the inverse Laplace transform and with a linear fit to the logarithm of the time domain signal. Two liquid phases were detected for the NaCl and dextrose solutions at lower temperatures, and assigned to low and high concentrated solution domains. The high concentrated solution domain was found to be present between -30 and -5 °C in the NaCl solution and between -55 and -5 °C in the dextrose solution...
May 4, 2017: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/28468893/continuous-renal-replacement-therapy-a-potential-source-of-calories-in-the-critically-ill
#14
Andrea M New, Erin M Nystrom, Erin Frazee, John J Dillon, Kianoush B Kashani, John M Miles
Background: Overfeeding can lead to multiple metabolic and clinical complications and has been associated with increased mortality in the critically ill. Continuous venovenous hemofiltration (CVVH) represents a potential source of calories that is poorly recognized and may contribute to overfeeding complications.Objective: We aimed to quantify the systemic caloric contribution of acid-citrate-dextrose regional anticoagulation and dextrose-containing replacement fluids in the CVVH circuit.Design: This was a prospective study in 10 critically ill adult patients who received CVVH from April 2014 to June 2014...
May 3, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28463940/clinical-impact-of-rapid-intravenous-rehydration-with-dextrose-serum-in-children-with-acute-gastroenteritis
#15
Marta Sendarrubias, Marta Carrón, Juan Carlos Molina, Maria Ángeles Pérez, Rafael Marañón, Andrea Mora
OBJECTIVES: We designed a study to compare rapid intravenous rehydration based on 0.9% normal saline (NS) or on NS + glucose 2.5% serum (SGS 2.5%) in patients with dehydration secondary to acute gastroenteritis. Our hypothesis is that the addition of glucose 2.5% serum (SGS 2.5%) to 0.9% saline solution could reduce the proportion of hospital admissions and return emergency visits in these patients. The secondary objective was to identify differences in the evolution of blood glucose and ketonemia between the groups...
May 1, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/28463583/decolorization-of-acid-disperse-and-reactive-dyes-by-trametes-versicolor-cbr43
#16
Seung-Ok Yang, Hong Sodaneath, Jung-In Lee, Hyekyeng Jung, Jin-Hee Choi, Hee Wook Ryu, Kyung-Suk Cho
The mycoremediation has been considered as a promising method for decolorizing dye wastewater. To explore new bioresource for mycoremediation, a new white-rot fungus that could decolorize various dyes commonly used in textile industries was isolated, and its ligninolytic enzyme activity and decolorization capacity were characterized. The isolated CBR43 was identified as Trametes versicolor based on the morphological properties of its fruit body and spores, as well as through partial 18S rDNA gene sequences...
May 2, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28462176/assessment-of-effectiveness-of-fluconazole-and-clotrimazole-in-treating-oral-candidiasis-patients-a-comparative-study
#17
R C Jagat Reddy, S Jeelani, P Duraiselvi, M Kandasamy, G Suresh Kumar, R Azhal Vel Pandian
AIMS: One of the most common fungal infections infecting humans is Candidiasis. Belonging to the group of opportunistic infections, it often affects individuals with various debilitating diseases. Fluconazole and clotrimazole are two of the commonly used anti-fungal agents for the treatment of oral candidiasis. Hence, we planned this study to evaluate the effectiveness of fluconazole and clotrimazole in the treatment of patients suffering from candidiasis. MATERIALS AND METHODS: A total of 180 participants were enrolled in the present study...
March 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28460873/long-term-therapeutic-effects-of-dextrose-prolotherapy-in-patients-with-hypermobility-of-the-temporomandibular-joint-a-single-arm-study-with-1-4-years-follow-up
#18
H Refai
The aim was to analyse the short-term and long-term therapeutic efficacy of dextrose prolotherapy for dislocation or subluxation (hypermobility) of the temporomandibular joint (TMJ). Sixty-one patients with symptomatic hypermobility of the TMJ were included in this single-arm prospective study, in which they were each given four sessions of intra-articular and pericapsular injections six weeks apart. Each injection comprised 10% dextrose/mepivacaine solution 3ml. Clinical outcomes including severity of pain on movement according to the numerical rating scale (NRS), maximal interincisal opening, clicking, and frequency of locking were measured before treatment (T1), during treatment (T2) (just before the third session of injections), at the short-term follow-up (T3) (three months after treatment), and at the long-term follow-up (T4) (1-4 years after treatment)...
April 28, 2017: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/28458692/control-of-passion-fruit-fungal-diseases-using-essential-oils-extracted-from-rosemary-rosmarinus-officinalis-and-eucalyptus-eucalyptus-agglomerata-in-egerton-university-main-campus-njoro-kenya
#19
Paul Njenga Waithaka, Eliud Mugu Gathuru, Benson Muriuki Githaiga, Salome Nduta Kimani
Growth of fruits which form an important part of human diet has been jeopardized by the many fungal diseases that are present today. This study was conceived to isolate the most common fungal pathogens in passion fruits. Fungi were isolated using potato dextrose agar in addition to characterization using morphological, cultural, and biochemical means. Extraction of essential oils from rosemary (Rosmarinus officinalis) and eucalyptus (Eucalyptus agglomerata) was done. Before carrying the sensitivity test of essential oils to the fungal isolates, constituents of the essential oils were determined...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28452021/biodegradation-of-thermally-treated-low-density-polyethylene-by-fungus-rhizopus-oryzae-ns-5
#20
Shraddha Awasthi, Neha Srivastava, Tripti Singh, D Tiwary, Pradeep Kumar Mishra
Polythene is considered as one of the important object used in daily life. Being versatile in nature and resistant to microbial attack, they effectively cause environmental pollution. In the present study, biodegradation of low-density polyethylene (LDPE) have been performed using fungal lab isolate Rhizopus oryzae NS5. Lab isolate fungal strain capable of adhering to LDPE surface was used for the biodegradation of LDPE. This strain was identified as Rhizopus oryzae NS5 (Accession No. KT160362). Fungal growth was observed on the surface of the polyethylene when cultured in potato dextrose broth at 30 °C and 120 rpm, for 1 month...
May 2017: 3 Biotech
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