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https://www.readbyqxmd.com/read/29035602/effect-of-pre-exercise-ingestion-of-modified-amylomaize-starch-on-glycemic-response-while-cycling
#1
Rachel B Parks, Hector F Angus, Douglas S King, Rick Sharp
Amylomaize-7 is classified as a resistant corn starch and is 68% digestible. When modified by partial hydrolysis in ethanol and hydrochloric acid, its digestibility is 92%, yet retains its low glycemic and insulinemic properties. The purpose of this study was to characterize the metabolic response when modified amylomaize-7 or dextrose is consumed in the hour before exercise, and to compare the effect on performance of a brief high-intensity cycling trial. Ten male trained cyclists were given 1 g/kg body mass of dextrose (DEX) or modified amylomaize-7 (AMY-7) or a flavored water placebo (PL) 45 min prior to exercise on a cycle ergometer...
October 16, 2017: International Journal of Sport Nutrition and Exercise Metabolism
https://www.readbyqxmd.com/read/29027706/regional-citrate-anticoagulation-with-calcium-replacement-in-pediatric-apheresis
#2
Katie Sigler, Ji Lee, Poyyapakkam Srivaths
PURPOSE: The objective of this retrospective analysis was to present our single-center experience with intravenous (IV) calcium replacement and regional citrate anticoagulation in pediatric apheresis therapy with the aim of developing a standard operating procedure to minimize symptomatic hypocalcemia. METHODS: We analyzed apheresis procedures in patients <18 years of age over a 2-year time period (Jan 2012 to Dec 2014). Procedures in tandem with other extracorporeal therapies, including continuous renal replacement therapy, extracorporeal liver support, and extracorporeal membrane oxygenation, were excluded...
October 13, 2017: Journal of Clinical Apheresis
https://www.readbyqxmd.com/read/29026306/enhanced-antifungal-activity-of-voriconazole-loaded-nanostructured-lipid-carriers-against-candida-albicans-with-a-dimorphic-switching-model
#3
Baocheng Tian, Qi Yan, Juan Wang, Chen Ding, Sixiang Sai
Candida commonly adheres to implanted medical devices and forms biofilms. Due to the minimal activity of current antifungals against biofilms, new drugs or drug-delivery systems to treat these persistent infections are urgently needed. In the present investigation, voriconazole-loaded nanostructured lipid carriers (Vrc-NLCs) were formulated for enhanced drug-delivery efficiency to C. albicans to increase the antifungal activity of Vrc and to improve the treatment of infectious Candida diseases. Vrc-NLCs were prepared by a hot-melt, high-pressure homogenization method, and size distribution, ζ-potential, morphology, drug-encapsulation efficiency, drug loading, and physical stability were characterized...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29017019/results-of-combined-single-session-arthrocentesis-and-dextrose-prolotherapy-for-symptomatic-temporomandibular-joint-syndrome-a-case-series
#4
Burak Cezairli, Efe Can Sivrikaya, Mehmet Melih Omezli, Ferhat Ayranci, Neslihan Seyhan Cezairli
OBJECTIVE: Arthrocentesis and prolotherapy are nonsurgical treatments for temporomandibular joint (TMJ) diseases. This study aimed to evaluate the treatment of hypermobility, pain, and displacement of the TMJ by consecutively performing arthrocentesis and prolotherapy in the same session. MATERIALS AND METHODS: In this study, 10 adults with disc displacement and painful, hypermobile TMJ were selected. Arthrocentesis and prolotherapy were consecutively performed using a 30% dextrose solution that was simultaneously injected into five areas: posterior disc attachment, superior joint space, superior and inferior capsular attachments, and stylomandibular ligament...
October 10, 2017: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/28992337/occurrence-and-species-distribution-of-pathogenic-mucorales-in-unselected-soil-samples-from-france
#5
B Mousavi, J M Costa, P Arné, J Guillot, R Chermette, F Botterel, E Dannaoui
Mucormycosis is a life-threatening invasive fungal disease that affects a variety of patient groups. Although Mucorales are mostly opportunistic pathogens originating from soil or decaying vegetation, there are currently few data on prevalence of this group of fungi in the environment. The aim of the present study was to assess the prevalence and diversity of species of Mucorales from soil samples collected in France. Two grams of soil were homogenized in sterile saline and plated on Sabouraud dextrose agar and RPMI agar supplemented with itraconazole or voriconazole...
July 28, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28991272/%C3%A3-ndice-gluc%C3%A3-mico-y-carga-gluc%C3%A3-mica-en-el-fruto-de-la-especie-vegetal-opuntia-ficus-indica
#6
María de Jesús Ibarra-Salas, Hilda Irene Novelo-Huerta, Marcela Alejandra De León-Salas, Mayra Elisa Sánchez-Murillo, María Del Carmen Mata-Obregón, Aurora de Jesús Garza-Juárez
other: Existe evidencia que apoya la utilidad clínica de que el índice glucémico (IG) y la carga glucémica (CG) podrían ser importantes en la prevención de enfermedades crónicas. Objetivo: Determinar el IG y la CG del fruto de Opuntia ficus-indica (tuna). Metodología: Se realizó un estudio analítico, transversal, con 25 voluntarios sanos con valores normales de índice de masa corporal, glucosa, hemoglobina glucosilada, colesterol y triglicéridos séricos, que mediante consentimiento informado aceptaron participar...
2017: Revista de Investigación Clínica; Organo del Hospital de Enfermedades de la Nutrición
https://www.readbyqxmd.com/read/28985713/patients-with-glycogen-storage-diseases-undergoing-anesthesia-a-case-series
#7
Carmelina Gurrieri, Juraj Sprung, Toby N Weingarten, Mary E Warner
BACKGROUND: Glycogen storage diseases are rare genetic disorders of glycogen synthesis, degradation, or metabolism regulation. When these patients are subjected to anesthesia, perioperative complications can develop, including hypoglycemia, rhabdomyolysis, myoglobinuria, acute renal failure, and postoperative fatigue. The objective of this study was to describe the perioperative course of a cohort of patients with glycogen storage diseases. METHODS: This is a retrospective review of patients with glycogen storage diseases undergoing anesthetic care at our institution from January 1, 1990, through June 30, 2015 to assess perioperative management and outcomes...
October 6, 2017: BMC Anesthesiology
https://www.readbyqxmd.com/read/28982951/in-vitro-synergy-of-natamycin-and-voriconazole-against-clinical-isolates-of-fusarium-candida-aspergillus-and-curvularia-spp
#8
Swatishree Sradhanjali, Bandana Yein, Savitri Sharma, Sujata Das
AIM: To determine the minimum inhibitory concentrations (MICs) of voriconazole and natamycin, alone and in combination, against the clinical isolates of Fungus and to evaluate the synergy between the drugs in an experimental in vitro study. METHODS: In an experimental in vitro study, clinical isolates of Fusarium, Aspergillus, Candida and Curvularia spp were maintained on Sabouraud Dextrose Agar and used for the study. The MICs of natamycin and voriconazole, used alone and in combination, were evaluated by checkerboard microdilution technique based on the standard protocol proposed by the Clinical Laboratory Standards Institute...
October 5, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28980027/-insertional-achilles-tendinopathy-differentiated-diagnostics-and-therapy
#9
REVIEW
S F Baumbach, M Braunstein, M G Mack, F Maßen, W Böcker, S Polzer, H Polzer
Achilles tendinopathy at the calcaneal insertion is classified into insertional tendinopathy, retrocalcaneal and superficial bursitis. The aim of this study was to present the current evidence on conservative and surgical treatment of insertional tendinopathy of the Achilles tendon. Conservative first-line therapy includes reduction of activity levels, administration of non-steroidal anti-inflammatory drugs (NSAID), adaptation of footwear, heel wedges and orthoses or immobilization. In addition, further conservative therapy options are also available...
October 4, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28979539/co-trimoxazole-induced-hypoglycaemia-in-an-immunosuppressed-intensive-care-patient
#10
Katherine A Richards, Simon Raby
An 18-year-old female inpatient on a neurosciences intensive care unitwith new onset super-refractory epilepsy became hypoglycaemic 48 h after commencing co-trimoxazole. She had been placed on this for prophylaxis against Pneumocystis jiroveci infection in the context of significant immunosuppression with high-dose corticosteroid therapy. In order to maintain glucose control, she required a continuous infusion of 10% dextrose at rates of 15-25 ml/h. Recurrent attempts to wean this were limited by further hypoglycaemia, until she spontaneously regained normoglycaemia after 73 days...
February 2017: J Intensive Care Soc
https://www.readbyqxmd.com/read/28977047/antifungal-activity-of-linalool-in-cases-of-candida-spp-isolated-from-individuals-with-oral-candidiasis
#11
I J Dias, E R I S Trajano, R D Castro, G L S Ferreira, H C M Medeiros, D Q C Gomes
This study analyzed the antifungal activity of phytoconstituents from linalool on Candida spp. strains, in vitro, isolated from patients with clinical diagnoses of oral candidiasis associated with the use of a dental prosthesis. Biological samples were collected from 12 patients using complete dentures or removable partial dentures and who presented mucous with diffuse erythematous or stippled features, indicating a clinical diagnosis of candidiasis. To identify fungal colonies of the genus Candida, samples were plated onto CHROMagar Candida®...
September 28, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28967446/-production-of-infectious-units-of-isaria-fumosorosea-hypocreales-cordycipitaceae-from-different-indigenous-isolates-of-northeastern-mexico-using-3-propagation-strategies
#12
Fatima L Gandarilla-Pacheco, Lilia H Morales-Ramos, Benito Pereyra-Alférez, Myriam Elías-Santos, Isela Quintero-Zapata
The aim of this study was to evaluate the production of blastospores and conidia of different native isolates and a strain of Isaria fumosorosea using different propagation techniques. Two liquid culture media of casamino acids and peptone as nitrogen sources and glucose as carbon source for both media cultures were respectively used in the production of blastospores, while for the production of conidia, the fungi were grown in potato dextrose agar; from these cultures, solutions of conidia to a concentration of 1×10(6) per milliliter were prepared to inoculate flasks with Sabouraud dextrose broth for the liquid phase of the biphasic culture, also known as preculture...
September 26, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28963679/clinical-practice-nausea-and-vomiting-in-acute-gastroenteritis-physiopathology-and-management
#13
REVIEW
Beatrice C Canziani, Peter Uestuener, Emilio F Fossali, Sebastiano A G Lava, Mario G Bianchetti, Carlo Agostoni, Gregorio P Milani
Currently recommended management of acute gastroenteritis is supportive. Although the affected children habitually have vomiting, recommendations do not focus on the correction of this symptom. In this condition, elevated ketone bodies and stimuli initiated by gut mucosa damage produced by the enteral pathogen likely underlay nausea and vomiting. As compared to 0.9% saline, intravenous administration of a dextrose-containing bolus of 0.9% saline is associated with a greater reduction of circulating ketones and a shorter duration of nausea and vomiting...
September 30, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28962618/high-density-lipoproteins-attenuate-high-glucose-impaired-endothelial-cell-signaling-and-functions-potential-implications-for-improved-vascular-repair-in-diabetes
#14
Xing Chen, My-Ngan Duong, Peter J Psaltis, Christina A Bursill, Stephen J Nicholls
BACKGROUND: Abnormalities of endothelial cell function are proposed to be a critical factor underlying adverse cardiovascular outcomes in the setting of hyperglycaemia. While high-density lipoproteins (HDL) have been demonstrated to be cardioprotective, the impact on the endothelium in hyperglycaemia has not been fully elucidated. METHODS: Human umbilical vein endothelial cells (HUVECs) were exposed to high-glucose conditions using dextrose, the main isoform of glucose, and native HDL...
September 29, 2017: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/28959796/pseudohyphae-formation-in-candida-glabrata-due-to-co2-exposure
#15
E Sasani, S Khodavaisy, S Agha Kuchak Afshari, S Darabian, F Aala, S Rezaie
BACKGROUND AND PURPOSE: Formation of pseudohyphae is considered a virulence factor in Candida species. Generally, Candida glabrata grows as budding yeast cells; however, reports illustrated that C. glabrata could form pseudohyphal cells in response to some stimuli. In this study, we provided insight into the ability of C. glabrata in forming pseudohyphal cells under different levels of carbon dioxide (CO2). MATERIALS AND METHODS: Candida glabrata reference strain (ATCC 90030) was used in this study...
December 2016: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/28959792/overexpression-of-mdr-1-and-cdr-2-genes-in-fluconazole-resistance-of-candida-albicans-isolated-from-patients-with-vulvovaginal-candidiasis
#16
K Khosravi Rad, M Falahati, M Roudbary, S Farahyar, S Nami
BACKGROUND AND PURPOSE: Candida albicans (C. albicans) is an opportunistic fungus that can colonize women's mucosal epithelial cell surfaces, causing vulvovaginitis in specific circumstances. The major genes contributing to drug resistance in C. albicans are the candida drug resistance (CDR) and multi drug resistance (MDR) genes. The purpose of this study was to evaluate the CDR-2 and MDR-1 gene expression patterns in C. albicans strains isolated from patients with recurrent vulvovaginal candidiasis...
December 2016: Advances in Medical Mycology (Iran)
https://www.readbyqxmd.com/read/28956350/construction-of-a-genetic-linkage-map-and-qtl-mapping-of-agronomic-traits-in-auricularia-auricula-judae
#17
Li-Xin Lu, Fang-Jie Yao, Peng Wang, Ming Fang, You-Min Zhang, Wei-Tong Zhang, Xiang-Hui Kong, Jia Lu
Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A...
October 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28955647/purification-characterization-and-statistical-optimization-of-a-thermostable-%C3%AE-amylase-from-desert-actinobacterium-streptomyces-fragilis-da7-7
#18
Krishnasamy Nithya, Chinnasamy Muthukumar, Shine Kadaikunnan, Naiyf S Alharbi, Jamal M Khaled, Dharumadurai Dhanasekaran
In this study, preliminary screening revealed that of 134 desert soil actinobacterial isolates, only 43 isolates produced amylase. Among these, an isolate DA7-7, which was identified as Streptomyces fragilis DA7-7, showed a prominent zone of clearance and significant amount of α-amylase production. The pre-optimization studies showed varying physicochemical and nutrients properties of the medium influenced the enzyme production significantly. Consequently, central composite design was employed with the selected variables (pH, temperature, dextrose, and peptone) for α-amylase production...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28955616/characterization-of-ligninolytic-enzyme-production-in-white-rot-wild-fungal-strains-suitable-for-kraft-pulp-bleaching
#19
Rosa María Damián-Robles, Agustín Jaime Castro-Montoya, Jaime Saucedo-Luna, Ma Soledad Vázquez-Garcidueñas, Marina Arredondo-Santoyo, Gerardo Vázquez-Marrufo
Fungal strains identified by phylogenetic analysis of the ITS rDNA region as Trametes versicolor (CMU-TA01), Irpex lacteus (CMU-84/13), and Phlebiopsis sp. (CMU-47/13) are able to grow on and bleach kraft pulp (KP) in a simple solid-state fermentation (SSF) assay conducted in Petri dishes. Kappa number reductions obtained with Phlebiopsis sp. (48.3%), T. versicolor (43%), and I. lacteus (39.3%), evidence their capability for lignin breakdown. Scanning electron microscopy images of KP fibers from SSF assays demonstrated increased roughness and striation, evidencing significant cell wall modification...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28948954/estimation-of-biofilm-proteinase-phospholipase-production-of-the-candida-species-isolated-from-the-oropharyngeal-samples-in-hiv-infected-patients
#20
Vicky Lahkar, Lahari Saikia, Saurav J Patgiri, Reema Nath, Partha Pratim Das
BACKGROUND & OBJECTIVES: Candida, the most common opportunistic infection in acquired immunodeficiency syndrome (AIDS), attributes its pathogenicity to its virulence factors, mainly the biofilms, the proteinases and the phospholipases. There is a significant interplay of these factors during the HIV infection. This study was aimed to estimate the biofilm, proteinase and phospholipase production in Candida species isolated from the oropharyngeal samples in the HIV-infected patients. METHODS: A total of 126 consecutive HIV-positive patients were screened for Candida growth using oropharyngeal swabs...
May 2017: Indian Journal of Medical Research
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