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https://www.readbyqxmd.com/read/29926312/biocompatibility-of-a-new-pd-solution-for-japan-reguneal%C3%A2-measured-as-in-vitro-proliferation-of-fibroblasts
#1
Bart Dioos, Goedele Paternot, Rose-Marie Jenvert, Annick Duponchelle, Mark R Marshall, Migaku Nakajima, Edward Ramirez Ganoza, James A Sloand, Anders P Wieslander
BACKGROUND: The aim of this study was to investigate in vitro biocompatibility of Reguneal™, a new bicarbonate containing peritoneal dialysis fluid (PDF) for Japan, and compare it with other PDFs available in that country. METHODS: We assessed basal cytotoxicity using in vitro proliferation of cultured fibroblasts, L-929, determining the quantity of living cells by the uptake of Neutral Red. Levels of ten glucose degradation products (GDPs) were measured by a validated ultrahigh-performance liquid chromatography method in combination with an ultraviolet detector...
June 20, 2018: Clinical and Experimental Nephrology
https://www.readbyqxmd.com/read/29924372/in-vitro-combination-of-antifungal-agents-against-malassezia-pachydermatis
#2
Karine B Schlemmer, Francielli P K de Jesus, Erico S Loreto, Julia B Farias, Sydney H Alves, Laerte Ferreiro, Janio M Santurio
The yeast Malassezia pachydermatis is a common commensal and occasional opportunistic pathogen of theskin microbiota of animals and humans. In this study, the susceptibility of M. pachydermatis isolates to fluconazole (FLC), itraconazole (ITZ), ketoconazole (KTZ), clotrimazole (CLZ), and miconazole (MCZ) alone and in combination with terbinafine (TRB), nystatin (NYS), and caspofungin (CSP) was evaluated in vitro based on the M27-A3 technique and the checkerboard microdilution method using Sabouraud dextrose broth with 1% tween 80 (SDB)...
June 19, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29924230/presence-of-candida-spp-and-candidiasis-in-liver-transplant-patients
#3
Samantha Bertoncello Dos Santos, Clarice Elvira Saggin Sabadin, Débora Nunes Mario, Lilian Rigo, Dulce Aparecida Barbosa
BACKGROUND: Candidiasis is the most common opportunistic fungal infection of the oral cavity caused by fungi of the genus Candida and usually associated with immunosuppressed individuals. OBJECTIVES: To evaluate the presence of oral candidiasis and identify the presence of Candida spp. in liver transplant recipients and assess the association between the presence of the fungus and sociodemographic variables, dietary habits and environmental exposure. METHODS: A cross-sectional study was performed with 49 patients who had undergone liver transplants at Hospital São Vicente de Paulo in Passo Fundo - RS...
June 2018: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/29923768/intrarenal-ghrelin-receptor-inhibition-ameliorates-angiotensin-ii-dependent-hypertension-in-rats
#4
Brandon A Kemp, Nancy L Howell, Shetal H Padia
The intrarenal ghrelin receptor (GR) is localized to collecting duct (CD) cells where it increases αENaC-dependent sodium reabsorption in rodents. We hypothesized that chronic GR inhibition with intrarenal GR siRNA lowers blood pressure (BP) in Angiotensin II-dependent hypertension via reductions in αENaC-dependent sodium reabsorption. Uninephrectomized Sprague-Dawley rats (N=121) received subcutaneous osmotic pumps for chronic systemic delivery of Angiotensin II or vehicle (5% dextrose in water). Rats also received intrarenal infusion of vehicle, GR siRNA, or scrambled (SCR) siRNA...
June 20, 2018: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/29913332/formulation-of-maize-and-peanut-based-semi-synthetic-growth-media-for-the-ecophysiological-studies-of-aflatoxigenic-aspergillus-flavus-in-maize-and-peanut-agro-ecosystems
#5
Siti Nur Ezzati Yazid, Hemashangari Thanggavelu, Norlia Mahror, Jinap Selamat, Nik Iskandar Putra Samsudin
In studying the ecophysiology of fungal phytopathogens, several stages are involved (in vitro, greenhouse, in planta). Most in vitro studies extensively utilise the general growth media such as Potato Dextrose Agar and Malt Extract Agar. Although the crop components in these media serve as excellent carbon sources and yield luxuriant growth, they are not naturally contaminated with Aspergillus flavus and thus might result in under- or overestimation of its actual toxigenic potentials. Empirical data on the formulation of semi-synthetic growth medium mimicking the natural crop commonly contaminated by A...
June 9, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29911459/a-case-of-linezolid-induced-toxicity
#6
Philip E Tobias, Christy A Varughese, Amy P Hanson, Payal K Gurnani
Adverse effects of linezolid are typically limited to diarrhea, nausea, and headache when shorter durations are used; however, as extended durations of linezolid therapy are increasingly more common, additional monitoring parameters should be considered in these patients. We describe a unique case of hypoglycemia, lactic acidosis, and pancreatitis related to an extended duration of linezolid therapy. A 52-year-old woman presented with altered mental status, abdominal pain, and hypotension following six weeks of linezolid and ertapenem therapy...
January 1, 2018: Journal of Pharmacy Practice
https://www.readbyqxmd.com/read/29909704/the-effects-of-intravenous-dextrose-5-ringer-s-solution-and-oral-intake-on-the-duration-of-labor-stages-in-nulliparous-women-a-double-blind-randomized-controlled-trial
#7
Fatemeh Ahadi Yulghunlu, Fahimeh Sehhatie Shafaie, Mojgan Mirghafourvand, Hamideh Mohaddesi
BACKGROUND AND OBJECTIVES: Childbirth consists of a three stage period beginning with uterine contractions and ending in the expulsion of the placenta. The present study was conducted to compare the effects of Ringer's solution, dextrose solution 5% and oral intake on the duration of total labor and labor stages (primary outcome) and the frequency of oxytocin administration and prolonged labor (secondary outcome) in nulliparous women with spontaneous vaginal delivery. MATERIALS AND METHODS: The present randomized clinical trial was conducted on 201 nulliparous women in labor assigned with a randomized block design to three groups, each receiving either Ringer's solution plus oral fluids, dextrose solution 5% solution plus oral fluids or oral fluids alone...
June 18, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29908430/comparative-metagenomics-reveals-different-hydrocarbon-degradative-abilities-from-enriched-oil-drilling-waste
#8
Amanda P Napp, José Evandro S Pereira, Jorge S Oliveira, Rita C B Silva-Portela, Lucymara F Agnez-Lima, Maria C R Peralba, Fátima M Bento, Luciane M P Passaglia, Claudia E Thompson, Marilene H Vainstein
The oil drilling process generates large volumes of waste with inadequate treatments. Here, oil drilling waste (ODW) microbial communities demonstrate different hydrocarbon degradative abilities when exposed to distinct nutrient enrichments as revealed by comparative metagenomics. The ODW was enriched in Luria Broth (LBE) and Potato Dextrose (PDE) media to examine the structure and functional variations of microbial consortia. Two metagenomes were sequenced on Ion Torrent platform and analyzed using MG-RAST...
June 11, 2018: Chemosphere
https://www.readbyqxmd.com/read/29908269/the-effect-of-zataria-multiflora-boiss-essential-oil-on-the-growth-and-citrinin-production-of-penicillium-citrinum-in-culture-media-and-cheese
#9
Fateme Akrami Mohajeri, Ali Misaghi, Hamidreza Gheisari, Afshin Akhondzadeh Basti, Assieh Amiri, Sayyed Razi Ghalebi, Zahra Derakhshan, Roohollah Dehghani Tafti
The effect of Zataria multiflora Boiss Essential oil (EO) on the growth, spore production, and citrinin production of Penicellium citrinum PTCC 5304 in the culture media as well as Iranian ultra-filtered white cheese in brine was investigated. Radial growth and spore production on the potato dextrose agar (PDA) were effectively inhibited by EO in a dose-dependent manner. At 200 ppm, the radial growth and sporulation declined by 92% and 100%, respectively. The growth was completely prevented at 400 ppm of EO on PDA and the minimum fungicidal concentration (MFC) of the oil was estimated at 400 ppm...
June 13, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29897957/preclinical-characterization-of-the-jak-stat-inhibitor-sgi-1252-on-skeletal-muscle-function-morphology-and-satellite-cell-content
#10
Jacob R Sorensen, Jordan D Fuqua, Michael R Deyhle, Jacob Parmley, Caitlin Skousen, Chad Hancock, Allen C Parcell, Robert D Hyldahl
BACKGROUND: Recent studies have highlighted the JAK/STAT signaling pathway in the regulation of muscle satellite cell behavior. Herein we report preclinical studies designed to characterize the effects of a novel JAK/STAT inhibitor on plantar flexor skeletal muscle function, morphology, and satellite cell content. METHODS: The compound, SGI-1252, was administered orally (400mg/kg) in a 10% dextrose solution to wild type mice (n = 6) 3 times per week for 8 weeks...
2018: PloS One
https://www.readbyqxmd.com/read/29895521/physical-compatibility-of-plazomicin-with-select-i-v-drugs-during-simulated-y-site-administration
#11
Tomefa E Asempa, Lindsay M Avery, James M Kidd, Joseph L Kuti, David P Nicolau
PURPOSE: The results of a study to determine the physical compatibility of plazomicin sulfate solution during simulated Y-site administration with 92 i.v. drugs are reported. METHODS: Plazomicin injection solution (500 mg/10 mL) was diluted in 0.9% sodium chloride or 5% dextrose for injection to a final volume of 50 mL (final plazomicin concentration, 24 mg/mL), consistent with a 15-mg/kg dose administered to an 80-kg patient (i.e., 1,200 mg). All other i.v. drugs were reconstituted according to manufacturers' recommendations and diluted with 0...
June 12, 2018: American Journal of Health-system Pharmacy: AJHP
https://www.readbyqxmd.com/read/29895117/-analysis-of-etiology-and-in-vitro-drug-susceptibility-of-fungal-keratitis-in-northern-china
#12
Y Zhang, Z Q Wang, X G Sun
Objective: To analyze the etiology, drug susceptibility and etiological changes of fungal keratitis so as to to provide reference for clinical diagnosis and treatment. Methods: Retrospective case series study. A total of 5 654 copies of specimens for fungal culture were collected from Outpatients suspected of fungal keratitis in Beijing Tongren Ophthalmic Center during January 2007 and December 2016. Specimens were inoculated on Potato dextrose agar (PDA) slant medium, incubated at 30℃ and 40% humidity constantly for 3 to 10 days...
June 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29887987/insulin-degludec-overdose-in-an-adolescent-with-type-1-diabetes-proactive-management-including-monitoring-using-the-freestyle-libre-flash-glucose-monitoring-system
#13
Harmony Thompson, Helen Lunt, Cate Fleckney, Steven Soule
An adolescent with type 1 diabetes and a history of self-harm, which included intentional overdoses and insulin omission, presented with an insulin degludec overdose. She had been commenced on the ultra-long-acting insulin, degludec, with the aim of reducing ketoacidosis episodes in response to intermittent refusal to take insulin. Insulin degludec was administered under supervision as an outpatient. Because it was anticipated that she would attempt a degludec overdose at some stage, the attending clinicians implemented a proactive management plan for this (and related) scenarios...
2018: Endocrinology, Diabetes & Metabolism Case Reports
https://www.readbyqxmd.com/read/29887860/inactivation-of-spoilage-yeasts-by-mentha-spicata-l-and-m-%C3%A3-villosa-huds-essential-oils-in-cashew-guava-mango-and-pineapple-juices
#14
Erika T da Cruz Almeida, Isabella de Medeiros Barbosa, Josean F Tavares, José M Barbosa-Filho, Marciane Magnani, Evandro L de Souza
This study evaluated the efficacy of the essential oil from Mentha spicata L. (MSEO) and M . × villosa Huds. (MVEO) to inactivate Candida albicans, C. tropicalis, Pichia anomala and Saccharomyces cerevisiae in Sabouraud dextrose broth and cashew, guava, mango, and pineapple juices during 72 h of refrigerated storage. The effects of the incorporation of an anti-yeast effective dose of MSEO on some physicochemical and sensory characteristics of juices were evaluated. The incorporation of 3.75 μL/mL MSEO or 15 μL/mL MVEO caused a ≥5-log reductions in counts of C...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29885817/osteopontin-attenuates-inflammation-via-jak2-stat1-pathway-in-hyperglycemic-rats-after-intracerebral-hemorrhage
#15
Lei Gong, Anatol Manaenko, Ruiming Fan, Lei Huang, Budbazar Enkhjargal, DevinW McBride, Yan Ding, Jiping Tang, Xiaoqiu Xiao, John H Zhang
Acute intracerebral hemorrhage (ICH) complicated by hyperglycemia is associated with aggravation of post-stroke inflammation, leading to exacerbation of brain edema and predicting poor neurological outcomes and higher mortality of patients. Osteopontin (OPN) is a neuroprotective glycoprotein, which is able to attenuate brain injury induced by hemorrhagic stroke. In the current study we investigated whether OPN will decrease the inflammatory post-ICH response as well as attenuate brain edema and neurological deficits in hyperglycemic rats...
June 7, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29883193/analgesic-effect-and-potential-cumulative-benefit-from-caudal-epidural-d5w-in-consecutive-participants-with-chronic-low-back-and-buttock-leg-pain
#16
Liza Maniquis-Smigel, Kenneth Dean Reeves, Howard Jeffrey Rosen, John Lyftogt, Cassie Graham-Coleman, An-Lin Cheng, David Rabago
OBJECTIVES: Chronic low-back pain (CLBP) participants in a prior controlled study reported short-term pain relief after caudal epidural injection of 5% dextrose (D5W). This study assessed whether repeated caudal epidural injections of D5W results in serial short-term diminution of CLBP and progressive long-term decrease in pain and disability. DESIGN: Prospective uncontrolled study. SETTINGS/LOCATION: Outpatient pain clinic. SUBJECTS: Adults with CLBP with radiation to gluteal or leg areas...
June 8, 2018: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/29880750/effects-of-glutamine-on-gastric-emptying-of-low-and-high-nutrient-drinks-in-healthy-young-subjects-impact-on-glycaemia
#17
Yang T Du, Diana Piscitelli, Saima Ahmad, Laurence G Trahair, Jerry R Greenfield, Dorit Samocha-Bonet, Christopher K Rayner, Michael Horowitz, Karen L Jones
Glutamine is a potent stimulus for the release of glucagon-like peptide-1, which increases postprandial insulin and slows gastric emptying (GE). We determined the effects of glutamine on GE of, and glycaemic responses to, low- and high-nutrient drinks in eight healthy males (mean age 21.6 ± 0.7 years and BMI 22.9 ± 0.7 kg/m²). Participants were studied on four occasions on which they consumed either a low-nutrient (beef soup; 18 kcal) or high-nutrient (75 g dextrose; 255 kcal) drink, each with or without 30 g of glutamine (120 kcal), in a randomised, crossover design...
June 7, 2018: Nutrients
https://www.readbyqxmd.com/read/29879053/ultrasound-guided-perineural-injection-with-dextrose-for-treatment-of-radial-nerve-palsy-a-case-report
#18
Si-Ru Chen, Yu-Ping Shen, Tsung-Yen Ho, Liang-Cheng Chen, Yung-Tsan Wu
RATIONALE: Perineural injection with dextrose could be a novel intervention for peripheral entrapment neuropathy. However, this intervention has not been reported for treating radial nerve palsy (RNP). Here, we present a case with RNP having outstanding improvement after 2 sessions of ultrasound-guided perineural injection with 5% dextrose (D5W). PATIENT CONCERNS: A 62-year-old woman presented with difficulty and weakness in extension of her left wrist and fingers after she slept with the arm compressed against her body...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29875755/biocontrol-of-aspergillus-flavus-on-peanut-kernels-using-streptomyces-yanglinensis-3-10
#19
Qaiser Shakeel, Ang Lyu, Jing Zhang, Mingde Wu, Guoqing Li, Tom Hsiang, Long Yang
The bacterium, Streptomyces yanglinensis 3-10, shows promise in the control of many phytopathogenic fungi. In this study, S. yanglinensis and its antifungal substances, culture filtrate (CF3-10 ) and crude extracts (CE3-10 ), were evaluated for their activity in reducing growth and aflatoxin AFB1 production by Aspergillus flavus , both in vitro and in vivo on peanut kernels. The results showed that in dual culture conditions, S. yanglinensis reduced the mycelial growth of A. flavus about 41% as compared to control...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29872587/subcutaneous-rhytidhysteron-infection-a-case-report-from-south-india-with-literature-review
#20
Nagaraja Mudhigeti, Rashmi Patnayak, Usha Kalawat, Spoorthy Rekha C Yeddula
Rhytidhysteron is a saprophytic dematiaceous fungus which rarely infects humans. Though virtually all individuals are exposed, very few develop the disease. Only seven human cases are reported till date. The present case is the second case from South India. A 40-year-old immunocompetent female agricultural worker, presented with a swelling on the dorsum of the right hand. Fine needle aspiration cytology (FNAC) of the swelling revealed short, thick, branched septate fungal hyphae. The isolate was moderately slow growing; grayish white colonies were observed on Sabouraud's Dextrose Agar (SDA) slant...
April 2, 2018: Curēus
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