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https://www.readbyqxmd.com/read/29668941/robust-pleiotropic-drug-resistance-5-pdr5-mediated-multidrug-resistance-is-vigorously-maintained-in-saccharomyces-cerevisiae-cells-during-glucose-and-nitrogen-limitation
#1
Hadiar Rahman, Joshua Carneglia, Molly Lausten, Michael Robertello, John Choy, John Golin
Saccharomyces cerevisiae has sophisticated nutrient-sensing programs for responding to harsh environments containing limited nutrients. As a result, yeast cells can live in diverse environments, including animals, as a commensal or a pathogen. Because they live in mixed populations with other organisms that excrete toxic chemicals, it is of interest to know whether yeast cells maintain functional multidrug resistance mechanisms during nutrient stress. We measured the activity of Pdr5, the major Saccharomyces drug efflux pump under conditions of limiting nutrients...
April 16, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29668894/endoscopic-treatment-of-ileal-pouch-sinus
#2
Nan Lan, Bo Shen
Background and aims: Pouch sinus is a serious complication in patients undergoing ileal pouch-anal anastomosis. The aim of this study was to verify the efficacy and safety of endoscopic needle knife sinusotomy (NKSi) in the management of pouch sinus. Methods: All consecutive patients with a pouch sinus treated with NKSi from 2008 to 2016 were identified. The primary outcomes were complete healing of the sinus and pouch survival. Results: A total of 109 patients were included...
April 12, 2018: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/29661840/therapeutic-potential-of-a-novel-necrosis-inhibitor-7-amino-indole-in-myocardial-ischemia-reperfusion-injury
#3
In-Chang Hwang, Ju-Young Kim, Ji-Hyun Kim, Joo-Eun Lee, Ji-Yun Seo, Jae-Won Lee, Jonghanne Park, Han-Mo Yang, Soon-Ha Kim, Hyun-Jai Cho, Hyo-Soo Kim
Opening of mitochondrial permeability transition pore and Ca2+ overload are main contributors to myocardial ischemia-reperfusion injury, which paradoxically causes a wide variety of myocardial damage. We investigated the protective role of a novel necrosis inhibitor (NecroX-7; NecX) against myocardial ischemia-reperfusion injury using in vitro and in vivo models. H9C2 rat cardiomyoblasts and neonatal cardiomyocytes were exposed to hypoxia-reoxygenation stress after pre-treatment with NecX, vitamin C, a combination of vitamin C and E, N-acetylcysteine, an apoptosis inhibitor (Z-VAD-fmk), or cyclosporine A...
April 16, 2018: Hypertension
https://www.readbyqxmd.com/read/29659969/aspartame-consumption-for-12-weeks-does-not-affect-glycemia-appetite-or-body-weight-of-healthy-lean-adults-in-a-randomized-controlled-trial
#4
Kelly A Higgins, Robert V Considine, Richard D Mattes
Background: Low-calorie sweeteners are often used to moderate energy intake and postprandial glycemia, but some evidence indicates that they may exacerbate these aims. Objective: The trial's primary aim was to assess the effect of daily aspartame ingestion for 12 wk on glycemia. Effects on appetite and body weight were secondary aims. Methods: One hundred lean [body mass index (kg/m2): 18-25] adults aged 18-60 y were randomly assigned to consume 0, 350, or 1050 mg aspartame/d (ASP groups) in a beverage for 12 wk in a parallel-arm design...
April 1, 2018: Journal of Nutrition
https://www.readbyqxmd.com/read/29658051/strategies-to-enhance-the-production-of-pinoresinol-and-its-glucosides-by-endophytic-fungus-phomopsis-sp-xp-8-isolated-from-tu-chung-bark
#5
Jing Zhu, Lu Yan, Xiaoguang Xu, Yan Zhang, Junling Shi, Chunmei Jiang, Dongyan Shao
To improve the production yield of (+)-pinoresinol (Pin), (+)-pinoresinol monoglucoside (PMG), and (+)-pinoresinol diglucoside (PDG), different methods were conducted, including co-culture with resveratrol-producing Alternaria sp. MG1 spores and addition of Tu-chung in a medium at the start of cultivation, ultrasound treatment (40 kHZ, 10 min) on 5-day culture, and addition of ethanol and sodium butyrate on Day 3, followed by cultivation for an additional period of 2 days. At the end of the cultivation period (5 days), the liquid phase was collected for product analysis...
April 16, 2018: AMB Express
https://www.readbyqxmd.com/read/29651862/four-weeks-of-%C3%AE-alanine-supplementation-improves-high-intensity-game-activities-in-water-polo
#6
Gabriel Motta Pinheiro Brisola, Elvis de Souza Malta, Paulo Roberto Pereira Santiago, Luiz Henrique Palucci Vieira, Alessandro Moura Zagatto
OBJECTIVES: The present study aimed to investigate whether four weeks of β-alanine supplementation improves total distance covered, distance covered and time spent in different speed zones, and sprint numbers during a simulated water polo game. DESIGN: The study design was double-blind, parallel and placebo controlled. Eleven male water polo players participated in the study, divided randomly into two homogeneous groups (placebo and β-alanine groups). METHODS: The participants performed a simulated water polo game before and after the supplementation period (4 weeks)...
April 13, 2018: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/29642408/mucormycosis-battle-with-the-deadly-enemy-over-a-five-year-period-in-india
#7
Jagdish Chander, Mandeep Kaur, Nidhi Singla, R P S Punia, Surinder K Singhal, Ashok K Attri, Ana Alastruey-Izquierdo, Alberto M Stchigel, Jose F Cano-Lira, Josep Guarro
Mucormycosis is an emerging opportunistic fungal infection. Increasing immunocompromization, widespread use of antibacterial and antifungal agents (such as voriconazole prophylaxis), carcinomas, transplantation and lifestyle diseases such as diabetes are the main contributors to this situation. The predominant clinical manifestations of mucormycosis vary from host to host, with rhino-orbital-cerebral, pulmonary, cutaneous, and gastrointestinal infections being the most common. In India, the prevalence of mucormycosis is approximately 0...
April 6, 2018: Journal of Fungi (Basel, Switzerland)
https://www.readbyqxmd.com/read/29628184/the-two-bag-method-for-treatment-of-diabetic-ketoacidosis-in-adults
#8
Nathan L Haas, Roma Y Gianchandani, Kyle J Gunnerson, Benjamin S Bassin, Arun Ganti, Christopher Hapner, Caryn Boyd, James A Cranford, Sage P Whitmore
BACKGROUND: The "two-bag method" of management of diabetic ketoacidosis (DKA) allows for titration of dextrose delivery by adjusting the infusions of two i.v. fluid bags of varying dextrose concentrations while keeping fluid, electrolyte, and insulin infusion rates constant. OBJECTIVE: We aimed to evaluate the feasibility and potential benefits of this strategy in adult emergency department (ED) patients with DKA. METHODS: This is a before-and-after comparison of a protocol using the two-bag method operationalized in our adult ED in 2015...
April 5, 2018: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29625731/cost-analysis-of-treating-neonatal-hypoglycemia-with-dextrose-gel
#9
Matthew J Glasgow, Jane E Harding, Richard Edlin
OBJECTIVE: To evaluate the costs of using dextrose gel as a primary treatment for neonatal hypoglycemia in the first 48 hours after birth compared with standard care. STUDY DESIGN: We used a decision tree to model overall costs, including those specific to hypoglycemia monitoring and treatment and those related to the infant's length of stay in the postnatal ward or neonatal intensive care unit, comparing the use of dextrose gel for treatment of neonatal hypoglycemia with placebo, using data from the Sugar Babies randomized trial...
April 3, 2018: Journal of Pediatrics
https://www.readbyqxmd.com/read/29614970/a-novel-citrate-based-protocol-versus-heparin-anticoagulation-for-sustained-low-efficiency-dialysis-in-the-icu-safety-efficacy-and-cost
#10
Ming Wen, Claudius Küchle, Dominik Steubl, Robin Satanovskji, Uwe Heemann, Yana Suttmann, Susanne Angermann, Stephan Kemmner, Lisa Rehbehn, Monika Huber, Christine Hauser, Christoph Schmaderer, Anna-Lena Reichelt, Bernhard Haller, Lutz Renders
BACKGROUND: The high cost, complexity of the available protocols, and metabolic complications are the major barriers that impede the clinical utilization of regional citrate anticoagulation (RCA) for sustained low efficiency dialysis (SLED) in critically ill patients. By comparing a novel protocol for SLED using 30% citrate solution with common protocol using unfractionated heparin, this study aimed to provide new insights for clinical applications of RCA. METHODS: In this retrospective study, a total of 282 critically ill patients who underwent SLED with citrate and/or heparin anticoagulation in six adult ICUs were enrolled...
April 3, 2018: BMC Nephrology
https://www.readbyqxmd.com/read/29609940/functional-outcome-from-sacroiliac-joint-prolotherapy-in-patients-with-sacroiliac-joint-instability
#11
Martin D Hoffman, Vikram Agnish
OBJECTIVES: Examine the effectiveness of sacroiliac (SI) joint prolotherapy for SI joint instability, and characterize the patients most likely to benefit from this treatment. DESIGN: Retrospective cohort study. SETTING: Department of Veterans Affairs outpatient physical medicine clinic. INTERVENTIONS: Patients referred for low back pain and diagnosed with SI joint instability received a series of three SI joint prolotherapy injections (15% dextrose in lidocaine) at approximately a one-month interval...
April 2018: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/29605659/interscalene-brachial-plexus-bolus-block-versus-patient-controlled-interscalene-indwelling-catheter-analgesia-for-the-first-48-hours-after-arthroscopic-rotator-cuff-repair
#12
Jong-Ho Kim, Hyun Jung Koh, Dong Kyu Kim, Hyo-Jin Lee, Ki-Hyun Kwon, Kwang-Yeol Lee, Yang-Soo Kim
BACKGROUND: We sought to compare the efficacy of interscalene brachial plexus bolus blockade (IBPBB) and patient-controlled interscalene indwelling catheter analgesia (PCIA) for postoperative pain management within 48 hours postoperatively in patients undergoing arthroscopic rotator cuff repairs (ARCR). METHODS: Patients undergoing ARCR were randomized into 3 groups by postoperative analgesia method. The IBPBB group received a mixed solution of 16 mL of 0.75% ropivacaine and 4 mL of 2% lidocaine as a bolus postoperatively...
March 29, 2018: Journal of Shoulder and Elbow Surgery
https://www.readbyqxmd.com/read/29605495/-frequency-of-candida-in-root-canals-of-teeth-with-primary-and-persistent-endodontic-infections
#13
Angel Bernal-Treviño, Ana María González-Amaro, Verónica Méndez González, Amaury Pozos-Guillen
BACKGROUND: Microbiological identification in endodontic infections has focused mainly on bacteria without giving much attention to yeasts, which, due to their virulence factors, can affect the outcomes of root canal treatment. AIMS: To determine the frequency of Candida in anaerobic conditions in root canals with primary and persistent endodontic infection, as well as to evaluate a microbiological sampling method using aspiration compared to the traditional absorption method with paper points...
March 28, 2018: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/29605395/prophylactic-dextrose-gel-does-not-prevent-neonatal-hypoglycemia-a-quasi-experimental-pilot-study
#14
Sarah M Coors, Joshua J Cousin, Joseph L Hagan, Jeffrey R Kaiser
OBJECTIVE: To test the hypothesis that prophylactic dextrose gel administered to newborn infants at risk for hypoglycemia will increase the initial blood glucose concentration after the first feeding and decrease neonatal intensive care unit (NICU) admissions for treatment of asymptomatic neonatal hypoglycemia compared with feedings alone. STUDY DESIGN: This quasi-experimental study allocated asymptomatic at-risk newborn infants (late preterm, birth weight <2500 or >4000 g, and infants of mothers with diabetes) to receive prophylactic dextrose gel (Insta-Glucose; Valeant Pharmaceuticals North America LLC, Bridgewater, New Jersey); other at-risk infants formed the control group...
March 28, 2018: Journal of Pediatrics
https://www.readbyqxmd.com/read/29594464/heavy-metal-tolerance-in-marine-strains-of-yarrowia-lipolytica
#15
Ashok Bankar, Smita Zinjarde, Manisha Shinde, Gita Gopalghare, Ameeta Ravikumar
Heavy metal tolerance of two marine strains of Yarrowia lipolytica was tested on solid yeast extract peptone dextrose agar plates. Based on minimum inhibitory concentration esteems, it is inferred that the two strains of Y. lipolytica were tolerant to heavy metals such as Pb(II), Cr(III), Zn(II), Cu(II), As(V), and Ni(II) ions. The impact of various heavy metal concentrations on the growth kinetics of Y. lipolytica was likewise assessed. With increased heavy metal concentration, the specific growth rate was reduced with delayed doubling time...
March 29, 2018: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/29581608/physical-compatibility-of-micafungin-with-sodium-bicarbonate-hydration-fluids-commonly-used-with-high-dose-methotrexate-chemotherapy
#16
Logan C Joiner, Clay Tynes, John Arnold, Rachel R Miller, Greg Gorman
Purpose: The purpose of this study was to determine the physical compatibility of micafungin with commonly used concentrations of sodium bicarbonate hydration fluids administered via a Y-site connected to a central venous catheter (Y-site/CVC). Methods: Micafungin sodium (evaluated concentration of 1.5 mg/mL) was combined in a 3:1 (vehicle:drug) ratio with the following commonly used hydration vehicles: 40 mEq/L sodium bicarbonate in 5% dextrose in water with ¼ normal saline (40SB-D5W-1/4NS), 75 mEq/L sodium bicarbonate in D5W (75SB-D5W), and 154 mEq/L sodium bicarbonate in D5W (154SB-D5W)...
April 2018: Hospital Pharmacy
https://www.readbyqxmd.com/read/29579758/glucose-gel-in-infants-at-risk-for-transitional-neonatal-hypoglycemia
#17
Kartikeya Makker, Rana Alissa, Christopher Dudek, Laura Travers, Carmen Smotherman, Mark L Hudak
OBJECTIVE:  To evaluate whether glucose gel as a supplement to feedings in infants admitted to the newborn nursery at risk for neonatal hypoglycemia (NH) reduces the frequency of transfer to a higher level of care for intravenous dextrose treatment. STUDY DESIGN:  We revised our newborn nursery protocol for management of infants at risk for NH to include use of 40% glucose gel (200 mg/kg). Study population included late preterm, small and large for gestational age infants, and infants of diabetic mothers...
March 26, 2018: American Journal of Perinatology
https://www.readbyqxmd.com/read/29567595/the-ph-based-fed-batch-for-lipid-production-from-trichosporon-oleaginosus-with-crude-glycerol
#18
Jiaxin Chen, Xiaolei Zhang, Patrick Drogui, Rajeshwar Dayal Tyagi
In this study, it was found that the optimal pH for the growth of Trichosporon oleaginosus was related to the fermentation medium. A neutral or weak acid pH condition was optimal for the growth of Trichosporon oleaginosus in the extract-peptone-dextrose and wastewater sludge medium. Significant inhibition was observed at neutral pH in the wastewater sludge + crude glycerol medium due to the high soap content of the crude glycerol. By converting the soap to free fatty acid (FFA) at pH 5, the soap inhibition could be prevented...
March 13, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29564098/the-inhibitory-potential-of-zataria-multiflora-and-syzygium-aromaticum-essential-oil-on-growth-and-aflatoxin-production-by-aspergillus-flavus-in-culture-media-and-iranian-white-cheese
#19
Marzieh Moosavi-Nasab, Jalal Jamalian, Hana Heshmati, Soroush Haghighi-Manesh
Antifungal activity of essential oils (EOs) of Zataria multiflora (thyme) and Syzygium aromaticum (clove) against Aspergillus flavus growth and aflatoxin B1 production was studied in potato dextrose agar (PDA) and potato dextrose broth (PDB), as well as in Iranian white cheese as a food model. The results showed that the inhibitory potential of EOs in the PDB medium is more than PDA medium. Clove EO on PDB medium prevented fungal growth and aflatoxin B1 production at 300 and 100 ppm, respectively. However, the thyme EO was not able to inhibit fungal growth completely and showed the strongest inhibition effect at 400 ppm...
March 2018: Food Science & Nutrition
https://www.readbyqxmd.com/read/29563277/protein-carbamylation-in-peritoneal-dialysis-and-the-effect-of-low-glucose-plus-amino-acid-solutions
#20
Caitlin Trottier, Jeffrey Perl, Megan Freeman, Ravi Thadhani, Anders Berg, Sahir Kalim
Protein carbamylation is a post-translational urea-driven protein modification associated with mortality. Free amino acids (AAs) competitively inhibit protein carbamylation and parenteral AA therapy reduces carbamylation in hemodialysis (HD) patients. Peritoneal dialysis (PD) yields differences in urea clearance and AA balance compared with HD, but the influence of PD and intraperitoneal AA solutions on carbamylation is unclear. Thus, we first measured carbamylated albumin (C-Alb; a marker of carbamylation load) in 100 diabetic HD patients frequency-matched by age, sex, and race to 98 diabetic PD subjects from the IMPENDIA trial, which originally compared the metabolic effects of low-glucose PD solutions (incorporating icodextrin and AAs) to a control group (dextrose-only solutions)...
March 2018: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
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