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microbial contamination of eye drops

Eduardo Anitua, María de la Fuente, Ana Riestra, Jesús Merayo-Lloves, Francisco Muruzábal, Gorka Orive
PURPOSE: To analyze whether plasma rich in growth factors (PRGF) eye drops preserve their biological characteristics and activity after storage for 3 and 6 months at -20°C, at 4°C, and at room temperature for 72 hours, compared with fresh samples (t0). METHODS: Blood from 6 healthy donors was harvested and centrifuged to obtain PRGF free of leukocytes. Resulting PRGF eye drops were stored for 3 and 6 months at -20°C. At each time, 2 aliquots were maintained at room temperature or at 4°C for 72 hours...
September 2015: Cornea
Francesco Blasetti, Donatella Usai, Salvatore Sotgia, Ciriaco Carru, Stefania Zanetti, Antonio Pinna
INTRODUCTION: The study aimed to investigate whether the preparation, storage, and use of autologous serum in insulin syringes is microbiologically safe. METHODOLOGY: Blood samples (10 mL) were obtained from 10 volunteers. After centrifugation, the supernatant serum was removed and distributed in 5 sterile insulin syringes for each sample; syringes were numbered 0 to 4 and labelled with the subject's details. Syringes were immediately transported to the microbiology laboratory and stored in a refrigerator at +4°C...
January 2015: Journal of Infection in Developing Countries
Christy E Benson, Kathy L Rogers, Donny W Suh
PURPOSE: To investigate the contamination rates in patients undergoing strabismus surgery who received a single versus a dual application of povidone-iodine. METHODS: Patients undergoing strabismus surgery were divided into two groups of approximately equal size. Prior to surgery, the surgical sites for both groups were prepared by applying 4 drops of povidone-iodine 5% directly into the conjunctival fornices, after which the eyelashes were cleaned with cotton tips soaked with povidone-iodine 5%...
August 2014: Journal of AAPOS: the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus
Prajna Lalitha, Manoranjan Das, Patwari S Purva, Rajaratinam Karpagam, Manoharan Geetha, Jeganathan Lakshmi Priya, Kannan Naresh Babu
OBJECTIVE: To report the clinical presentation and outcomes of cluster postcataract Burkholderia cepacia complex endophthalmitis, the source of infection and clonal relatedness of the isolates. METHODS: This was a retrospective study on 13 patients who developed acute postoperative endophthalmitis, along with an infiltrate at the corneal section, after an uneventful cataract surgery with intraocular lens implantation. Aqueous aspirates, vitreous aspirates and environmental surveillance specimens were sampled...
November 2014: British Journal of Ophthalmology
Snježana Kaštelan, Martina Tomić, Kata Metež Soldo, Jasminka Salopek-Rabatić
The treatment goals for glaucoma are lowering the intraocular pressure and preservation of vision. Topical hypotensive drops are the standard form of therapy which is often associated with some symptoms of toxicity, ocular inflammation, allergy, or ocular surface disease (OSD). OSD is a common comorbidity in glaucoma patients, and its prevalence with glaucoma increases with age. Use of topical treatment could additionally increase symptoms of OSD mostly due to preservatives added to multidose medication bottles used to reduce the risk of microbial contamination...
2013: BioMed Research International
A Carron, M Samudio, F Laspina, N Fariña, R R Sanabria, D Cibils, L Ramirez, J Carron, H Mino de Kaspar
OBJECTIVE: To determine the efficacy of topical 0.3% ciprofloxacin in reducing conjunctival biota in patients undergoing cataract surgery. PATIENTS AND METHODS: Experimental, prospective, randomized, controlled and single-blind study. Forty-six eyes of 46 patients were randomized into 2 groups, the study group (n=23) received topical 0.3% ciprofloxacin one day before surgery for six times, and on the day of the surgery one drop every 15minutes starting one hour before surgery until 3 doses were completed...
September 2013: Archivos de la Sociedad Española de Oftalmología
Eduardo Anitua, Francisco Muruzabal, Ander Pino, Jesús Merayo-Lloves, Gorka Orive
PURPOSE: We evaluated whether plasma rich in growth factors eye drops maintain their composition and biological activity after storage for 3 months at -20°C and after storage at 4°C or room temperature (RT) for 24 hours, compared with samples obtained at time 0 (fresh samples). METHODS: Blood from 10 healthy donors was collected, centrifuged, and plasma rich in growth factors was prepared by avoiding the collection of the buffy coat. Eye drops were kept fresh or were stored at -20°C for 15, 30, and 90 days...
October 2013: Cornea
Emilia Ghelardi, Francesco Celandroni, Sokhna A Gueye, Sara Salvetti, Mario Campa, Sonia Senesi
PURPOSE: The aim of this study was to examine the antimicrobial activity and the preservative efficacy of a novel preservative solution containing sodium hydroxymethyl glycinate (SHMG) and edetate disodium (EDTA), which is used for preservation of some commercial ophthalmic formulations. METHODS: In vitro susceptibility assays were performed against several gram-positive (Staphylococcus aureus, Staphylococcus epidermidis, and Bacillus cereus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria representative of the microbial flora of epithelial surfaces or colonizing the conjunctiva, as well as against Candida albicans and Aspergillus niger...
July 2013: Journal of Ocular Pharmacology and Therapeutics
Pornchai Simaroj, Suvicha Kompreyarat, Pitak Santanirand, Kaevalin Lekhanont
OBJECTIVE: To determine the rate of anterior chamber contamination during phacoemulsification after applying 5% povidone-iodine prior to surgery. MATERIAL AND METHOD: One hundred eyes from 100 patients having phacoemulsification cataract surgery by a single surgeon at Ramathibodi Hospital between October 2010 and March 2011 were included in the present study. Povidone-iodine 5% solution eye drops were used in all patients at least three minutes before the operation...
May 2012: Journal of the Medical Association of Thailand, Chotmaihet Thangphaet
Chen-jiu Pang, Li-ya Wang, Sheng-tao Sun, Lei Han, Xiang Li
OBJECTIVE: The microbial culturing results were analysed from samples of the microkeratome blade and sponges in laser in situ keratomileusis (LASIK) procedures as for crossing the rational antibiotic eye drops for preventing infectious keratitis. METHOD: In this prospective study, 106 microkeratome blades and 212 sponges were cultured in routine LASIK procedure, at Excimer laser center in Henan Eye Institute During March to April 2009. Positive cultures were then sent for routine sensitivities and the results were analysed...
May 2012: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Yasushi Ikuno, Miki Sawa, Motokazu Tsujikawa, Fumi Gomi, Naoyuki Maeda, Kohji Nishida
PURPOSE: To compare the effect of a reduced concentration (1.25 %) of povidone-iodine (PI) eye drops, combined with 0.5 % topical levofloxacin (LVFX), with that of the standard of care (5 % PI) on conjunctival flora before intravitreal injections (IVT). METHODS: A prospective, randomized, single-blind clinical trial. One hundred eyes from 100 patients who underwent IVT were included. Eyes were randomly assigned to two groups and underwent different preparatory procedures before therapeutic IVT...
September 2012: Japanese Journal of Ophthalmology
Jerome Ozkan, Hua Zhu, Manal Gabriel, Brien A Holden, Mark D P Willcox
PURPOSE: Bacterial contamination of the contact lens surface has been demonstrated to cause corneal infiltrative events. A reduction in the rate of bacterially driven corneal infiltrative events associated with lens wear is one of the major goals of the contact lens industry. There is a concern over the potential of any antimicrobial strategy that there will be unwanted changes to the ocular microbiota or the development of resistance to the antimicrobial. The aim of this study was to investigate the effect of prophylactic topical antibiotic instillation during continuous wear of silicone hydrogel lenses on the normal ocular microbiota, the throat microbiota, and the ocular physiology...
March 2012: Optometry and Vision Science: Official Publication of the American Academy of Optometry
C Stefan, Adina Pop, Inga Cojocaru
Ophthalmic solutions are the main therapeutic methods used in the treatment of ocular diseases, including glaucoma. Preservatives are added in multidose solutions to reduce the risk of microbial contamination. Repeated use of topical medication, especially in patients treated for glaucoma, has many adverse effects especially on the ocular surface. This paper provides an overview of the role of preservatives in antiglaucoma preparations, and their impact on the ocular surface.
2011: Oftalmologia
C Stefan, Inga Cojocaru, Adina Pop
Topical eye-drops remain the cornerstone of treatment for ocular diseases, including glaucoma. Preservatives are added to multi-dose medication bottles to reduce the risk of microbial contamination, to extend the validity of the product and to allow an easily use of the bottles. However the repetitious use of ophthalmic medications containing preservatives, particularly when treating chronic diseases, has been linked to adverse effects and ocular surface disease (OSD). Therefore, this presentation provides an overview of glaucoma and OSD, the role of preservatives in ophthalmic preparations, and the impact of preservatives on the ocular surface...
2011: Oftalmologia
Alex Golberg, Michael Belkin, Boris Rubinsky
The purpose of this study was to examine the feasibility of using irreversible electroporation (IRE) as a non-chemical method for eliminating microorganisms of liquid drugs. The studied drug was a topical ophthalmic medication, a pharmaceutical field in which the problem of microbial contamination has not yet been adequately solved, especially in the case of eye drops prescribed for chronic use. Commercially available Hylo-Comod preservative-free eye drop solution was subjected to contamination with Escherichia coli bacteria (10(6) colony forming units/mL)...
2009: AAPS PharmSciTech
M S Kim, C Y Choi, J M Kim, H R Chang, H R Chung, H Y Woo
BACKGROUND/AIMS: To evaluate microbial contamination of multiply used preservative-free artificial tears packed in reclosable containers after daily use. METHODS: Subjects were provided with preservative-free artificial tears (Groups 1 and 2) and artificial tears containing a preservative (Group 3). After administration three times or more per 10 h, bottles were collected, and any remaining fluid in the bottles was cultured. A risk factor analysis for microbial contamination was performed by the use of univariate and multivariate analysis...
November 2008: British Journal of Ophthalmology
Renuka Srinivasan, Arvind Gupta, Subashini Kaliaperumal, Ramesh K Babu, Senthil Kumar Thimmarayan, Harish Narasimha Belgode
PURPOSE: To study the efficacy of adding vancomycin in irrigating solutions, in comparison to topical antibiotic given preoperatively for a day, during phacoemulsification, in reducing the anterior chamber (AC) contamination. SETTINGS AND DESIGN: This was a prospective, interventional, hospital-based study. MATERIALS AND METHODS: This was a study involving 400 eyes of 400 paitens, undergoing routine phacoemulsification between January 2004 and June 2006...
September 2008: Indian Journal of Ophthalmology
Mostafa Feghhi, Ali Zarei Mahmoudabadi, Manijeh Mehdinejad
The aim of the present study was to investigate the incidence of fungal and bacterial contaminations of in-use eye drop products in the teaching department of ophthalmology, Imam Khomeini Hospital, Ahvaz. Two hundred and eighty seven eye drop bottles were randomly collected at the end of day 1, day 2, day 3, day 4 and day 7 of use. The eye drop residues, swabs from internal caps and droppers were inoculated onto MacConkey agar, blood agar and Sabouraud's dextrose agar. The identification of the recovered organisms was accomplished using standard microbial identification techniques...
February 2008: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
M M Nentwich, K H M Kollmann, J Meshack, D R Ilako, U C Schaller
BACKGROUND/AIMS: Contaminated ophthalmic solutions represent a potential cause of avoidable ocular infection. This study aimed to determine the magnitude and pattern of microbial contamination of multi-dose ocular solutions at the Department of Ophthalmology, University of Nairobi, at the Kenyatta National Hospital, Kenya. METHODS: 101 vials were obtained for microbial examination after an average use of 2 weeks. The dropper tip and the residual eye drop were examined for contamination...
October 2007: British Journal of Ophthalmology
Ruth A Rosenthal, Sally L Buck, Cindy L Henry, Barry A Schlech
Products provided in multidose containers must be adequately preserved in order to prevent contamination during repeated use. The aim of this study was to evaluate the ability of a lubricant eye drop formulation without traditional preservatives to control and prevent contamination under extreme microbial conditions. Bottles of a new lubricant eye drop with a novel preservative system (SYSTANE FREE Lubricant Eye Drops, Alcon Laboratories, Inc., Fort Worth, TX) were stored at elevated (40 degrees C) and room temperature (25 degrees C) for up to 1 year...
December 2006: Journal of Ocular Pharmacology and Therapeutics
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