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https://www.readbyqxmd.com/read/27914366/rapid-and-low-cost-biosensor-for-the-detection-of-staphylococcus-aureus
#1
Ghadeer A R Y Suaifan, Sahar Alhogail, Mohammed Zourob
Staphylococcus aureus (S. aureus) is one of the most common etiological agents in hospital-acquired infections and food-borne illness. S. aureus toxins and virulence proteases often circulate in host blood vessels leading to life-threatening diseases. Standard identification approaches include bacterial culturing method, which takes several days. Other nucleic acid-based methods were expensive and required trained personnel. To surmount these limitations, a paper-based biosensor was developed. The sensing mechanism was based on the proteolytic activity of S...
November 21, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27555747/an-upright-eyedrop-bottle-accuracy-usage-of-excess-drops-and-contamination-compared-to-a-conventional-bottle
#2
Isaiah J Davies, Ninita H Brown, Joanne C Wen, Sandra S Stinnett, Kelsey Kubelick, Roma P Patel, Kristin L Benokraitis, Latoya Greene, Curry Cheek, Kelly W Muir
PURPOSE: This study tested the feasibility of using an upright eyedrop bottle (UEB), a device designed to assist patients with eyedrop placement without reclining their head. PATIENTS AND METHODS: Experienced eyedrop users were enrolled who answered "yes" to the question, "Do you ever have trouble getting your eyedrops in?" After being shown a multimedia presentation and answering a questionnaire regarding eyedrop usage, participants were observed instilling eyedrops...
2016: Clinical Ophthalmology
https://www.readbyqxmd.com/read/27524529/the-effects-of-povidone-iodine-ph-4-2-on-patients-with-adenoviral-conjunctivitis
#3
Hayrullah Yazar, Abdulhekim Yarbag, Mehmet Balci, Bahri Teker, Pelin Tanyeri
OBJECTIVE: To compare the efficacy of classical treatment and povidone-iodine treatment for adenoviral conjunctivitis. METHODS: This retrospective study was conducted at the Centre of Marmara Eye Health, Sakarya, Turkey, between January 2011 and February 2014, and comprised adult patients suffering from adenoviral conjunctivitis. The participants were randomly divided into two groups. Group I was given povidone-iodine solution while Group II was given the classical treatment and was taken as control...
August 2016: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/26714366/protocol-of-blood-serum-eye-drops
#4
Ioannis Katsakoulas, Claudia Lougovoi, Penelope Paraskevopoulou, Nikolaos Vougioukas
Keratoconjunctivitis sicca is one of the most frequent diagnoses for seeking eye care. Accumulated evidence over the past three decades has revealed a significant contribution of several molecules contained in tears at the homeostasis of the epithelium of the ocular surface. Therefore, autologous blood serum in the form of eye drops can provide substantial help in the management of keratoconjunctivitis sicca. Also making this a favorable treatment for keratoconjunctivitis sicca is the fact that this approach has become an insurance-covered benefit in some countries...
May 2015: International Journal of Pharmaceutical Compounding
https://www.readbyqxmd.com/read/26165790/preservation-of-biological-activity-of-plasma-and-platelet-derived-eye-drops-after-their-different-time-and-temperature-conditions-of-storage
#5
Eduardo Anitua, María de la Fuente, Ana Riestra, Jesús Merayo-Lloves, Francisco Muruzábal, Gorka Orive
PURPOSE: To analyze whether plasma rich in growth factors (PRGF) eye drops preserve their biological characteristics and activity after storage for 3 and 6 months at -20°C, at 4°C, and at room temperature for 72 hours, compared with fresh samples (t0). METHODS: Blood from 6 healthy donors was harvested and centrifuged to obtain PRGF free of leukocytes. Resulting PRGF eye drops were stored for 3 and 6 months at -20°C. At each time, 2 aliquots were maintained at room temperature or at 4°C for 72 hours...
September 2015: Cornea
https://www.readbyqxmd.com/read/25796721/-chemokines-new-insights-in-the-treatment-of-glaucoma-and-inflammatory-diseases-of-the-ocular-surface
#6
William Rostene, Alexandre Denoyer, Christophe Baudouin
The celebrations of the 200th anniversary of Claude Bernard's birth (1813) give us the opportunity to recall all the pioneer work carried out by the great physiologist, which still remains relevant in both scientific and medical fields. The description that tissues are "floating in a liquid milieu" bringing survival molecules and allowing homeostasis, is particularly illustrated in the physiopathology of glaucoma. Second leading cause of blindness worldwide, glaucoma is an optic neuropathy mainly related to an increase of intraocular pressure...
October 2013: Bulletin de L'Académie Nationale de Médecine
https://www.readbyqxmd.com/read/25787926/efficacy-and-safety-of-lomefloxacin-on-bacterial-extraocular-disease-in-the-horse
#7
Shuhei Hidaka, Mitsutoshi Kobayashi, Kunihide Ando, Yoshikazu Fujii
Lomefloxacin is a broad-spectrum fluoroquinolone antibiotic used for the treatment of bacterial extraocular disease. This study investigated the efficacy and safety of lomefloxacin eye drops for bacterial extraocular disease in horses. Lomefloxacin ophthalmic solution (0.3%) was instilled three times daily for 2-5 days in 65 horses diagnosed with bacterial extraocular disease based on clinical findings. Clinical observations and bacteriological examinations were performed at the start of treatment, 2 and 5 days after the start of treatment, and at the discontinuation or termination of treatment...
July 2015: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/25666544/characterization-of-a-novel-process-related-impurity-in-commercial-bendazac-lysine-eye-drops-by-lc-esi-qtof-ms-ms-and-nmr
#8
Peixi Zhu, Weifeng Yang, Liya Hong, Qiaoyuan Cheng, Lei Luo, Jiajia He, Juanjuan Chen
A simple and sensitive method was developed for characterization of two impurities from commercial bendazac lysine eye drops, by using liquid chromatography combined with electrospray ionization and QTOF mass analyzer (LC-ESI-QTOF MS/MS). A known impurity was characterized rapidly by comparison with the retention time and MS/MS data of the reference standard. Based on high resolution mass spectrometry (HRMS) and the nuclear magnetic resonance (NMR), a new impurity, purified by preparative HPLC, was identified as 2-(1,5-dibenzyl-1H-indazol-3-yloxy)acetic acid...
March 25, 2015: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/25596572/a-protocol-for-microbiologically-safe-preparation-storage-and-use-of-autologous-serum-eye-drops-in-low-income-countries
#9
Francesco Blasetti, Donatella Usai, Salvatore Sotgia, Ciriaco Carru, Stefania Zanetti, Antonio Pinna
INTRODUCTION: The study aimed to investigate whether the preparation, storage, and use of autologous serum in insulin syringes is microbiologically safe. METHODOLOGY: Blood samples (10 mL) were obtained from 10 volunteers. After centrifugation, the supernatant serum was removed and distributed in 5 sterile insulin syringes for each sample; syringes were numbered 0 to 4 and labelled with the subject's details. Syringes were immediately transported to the microbiology laboratory and stored in a refrigerator at +4°C...
January 2015: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/25495852/clinical-features-risk-factors-and-treatments-of-microsporidial-epithelial-keratitis
#10
Onsiri Thanathanee, Raweewan Athikulwongse, Orapin Anutarapongpan, Porntip Laummaunwai, Wanchai Maleewong, Pewpan Maleewong Intapan, Olan Suwan-Apichon
OBJECTIVE: To report the clinical manifestations, risk factors, and treatments of microsporidial epithelial keratitis in Thailand. METHODS: Twenty eyes of 19 patients were diagnosed and the clinical presentations, risk factors, and management were analyzed. RESULTS: Of 19 patients, six patients (32%) had no apparent risk factors. Predisposing factors included soil exposure (6/19, 32%), water contamination (6/19, 32%), and eye liner (1/19, 4%)...
2016: Seminars in Ophthalmology
https://www.readbyqxmd.com/read/25474811/decoding-three-dimensional-trajectory-of-executed-and-imagined-arm-movements-from-electroencephalogram-signals
#11
Jeong-Hun Kim, Felix Bießmann, Seong-Whan Lee
Decoding motor commands from noninvasively measured neural signals has become important in brain-computer interface (BCI) research. Applications of BCI include neurorehabilitation after stroke and control of limb prostheses. Until now, most studies have tested simple movement trajectories in two dimensions by using constant velocity profiles. However, most real-world scenarios require much more complex movement trajectories and velocity profiles. In this study, we decoded motor commands in three dimensions from electroencephalography (EEG) recordings while the subjects either executed or observed/imagined complex upper limb movement trajectories...
September 2015: IEEE Transactions on Neural Systems and Rehabilitation Engineering
https://www.readbyqxmd.com/read/25272883/development-studies-on-determination-of-preservatives-decomposition-products
#12
Beata Zabrzewska, Anna Chyła, Anna Bogdan
Preservatives are chemical substances whose role is to protect medicinal products against harmful changes caused by microorganisms. They are added to sterile medicinal products, such as eye drops and multidose solutions for injections, as well as to non-sterile products, such as water oral solutions, creams, gels, suppositories and capsules with liquid content. The most commonly used preservatives include: benzyl alcohol, butyl, ethyl, methyl and propyl p-hydroxybenzoates and their sodium salts. In medicinal products benzyl alcohol slowly oxidizes to benzaldehyde and benzoic acid while esters of p-hydroxybenzoic acid hydrolyze to p-hydroxybenzoic acid...
July 2014: Acta Poloniae Pharmaceutica
https://www.readbyqxmd.com/read/25248295/evaluation-of-eyedrop-administration-by-inexperienced-patients-after-cataract-surgery
#13
Jella Angela An, Oscar Kasner, Deborah Anne Samek, Valérie Lévesque
PURPOSE: To evaluate efficacy and safety of eyedrop administration after cataract surgery and to identify predictors of better technique in patients without previous eyedrop experience. SETTING: Department of Ophthalmology, McGill University, Jewish General Hospital, Montreal, Quebec, Canada. DESIGN: Prospective cross-sectional study. METHODS: Eyedrop-naïve postoperative cataract patients were consecutively recruited the day after cataract surgery...
November 2014: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/25173897/dual-application-versus-single-application-of%C3%A2-povidone-iodine-in-reducing-surgical-site-contamination-during-strabismus-surgery
#14
Christy E Benson, Kathy L Rogers, Donny W Suh
PURPOSE: To investigate the contamination rates in patients undergoing strabismus surgery who received a single versus a dual application of povidone-iodine. METHODS: Patients undergoing strabismus surgery were divided into two groups of approximately equal size. Prior to surgery, the surgical sites for both groups were prepared by applying 4 drops of povidone-iodine 5% directly into the conjunctival fornices, after which the eyelashes were cleaned with cotton tips soaked with povidone-iodine 5%...
August 2014: Journal of AAPOS: the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/25103649/mirror-hat-device-as-a-drop-delivery-aid-a-pilot-study
#15
M Hermina Strungaru, Jonathan Peck, Emma C Compeau, Graham E Trope, Yvonne M Buys
OBJECTIVE: To evaluate the effectiveness of a novel mirror-hat drop delivery aid. DESIGN: Prospective, observational study. PARTICIPANTS: Sixty eyes from 30 subjects with primary open-angle glaucoma or ocular hypertension attending the glaucoma unit at the Toronto Western Hospital. METHODS: The mirror-hat device consists of a magnifying mirror attached to a brimmed cap. The aid was provided to subjects who self-administered topical antiglaucoma medications...
August 2014: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/25081521/the-role-of-multi-purpose-solutions-in-prevention-and-removal-of-lipid-depositions-on-contact-lenses
#16
COMPARATIVE STUDY
Ngai Keung Tam, William G Pitt, Krystian X Perez, Erika Handly, Andrew A Glenn, John W Hickey, Brian G Larsen
The sorption and desorption of radiolabeled dipalmitoylphosphatidylcholine (DPPC) and cholesterol (CH) were measured on 5 types of commercial contact lenses. The lenses were soaked in vitro in an artificial tear fluid for 16h. The effects of borate buffered saline and two commercial multi-purpose lens-care solutions (MPSs) on reducing the lipid (DPPC and CH) sorption and increasing the lipid removal were examined. The results showed that silicone hydrogel (SiHy) lenses accumulated the most lipids, sorbing over an order of magnitude more than polymacon, a conventional hydrogel lens...
December 2014: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/25071531/interference-of-tonic-muscle-activity-on-the-eeg-a-single-motor-unit-study
#17
Gizem Yilmaz, Pekcan Ungan, Oğuz Sebik, Paulius Uginčius, Kemal S Türker
The electrical activity of muscles can interfere with the electroencephalogram (EEG) signal considering the anatomical locations of facial or masticatory muscles surrounding the skull. In this study, we evaluated the possible interference of the resting activity of the temporalis muscle on the EEG under conventional EEG recording conditions. In 9 healthy adults EEG activity from 19 scalp locations and single motor unit (SMU) activity from anterior temporalis muscle were recorded in three relaxed conditions; eyes open, eyes closed, jaw dropped...
2014: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/24947905/postoperative-endophthalmitis-due-to-burkholderia-cepacia-complex-from-contaminated-anaesthetic-eye-drops
#18
Prajna Lalitha, Manoranjan Das, Patwari S Purva, Rajaratinam Karpagam, Manoharan Geetha, Jeganathan Lakshmi Priya, Kannan Naresh Babu
OBJECTIVE: To report the clinical presentation and outcomes of cluster postcataract Burkholderia cepacia complex endophthalmitis, the source of infection and clonal relatedness of the isolates. METHODS: This was a retrospective study on 13 patients who developed acute postoperative endophthalmitis, along with an infiltrate at the corneal section, after an uneventful cataract surgery with intraocular lens implantation. Aqueous aspirates, vitreous aspirates and environmental surveillance specimens were sampled...
November 2014: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/24782371/-determination-of-enantiomeric-impurity-of-timolol-maleate-in-bulk-substances-and-eye-drops
#19
Qian-ying Zhu, Lu-shan Yu, Guo-gang Zheng, Shen-gu Xie, Qiao-feng Tao
OBJECTIVE: To determine the enantiomeric impurity contents of domestic timolol maleate in bulk drugs and eye drops. METHODS: Enantiomer impurity of timolol was assayed by chiral high performance liquid chromatography. The chromatographic conditions were as follows:chiralcel OD chiral column (4.6 mm ×150 mm, 5μm), detection wavelength:297 nm, mobile phase:hexane-isopropanol-diethylamine (480:20:1), column temperature:25 ℃, flow rate:1.0 ml/min, sample injection volume:5 μl...
March 2014: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
https://www.readbyqxmd.com/read/24441738/rotation-of-the-eyes-not-the-head-potentiates-the-postauricular-muscle-response
#20
Alison Cook, Robert Patuzzi
OBJECTIVE: The authors investigated how eye and head rotation modulate the human postauricular muscle response (PAMR), to determine the optimal strategy for potentiating the PAMR, or minimizing it to reduce contamination of small neurogenic responses. DESIGN: The authors recorded the PAMR evoked by binaural bipolar clicks (50 dB SL, 360 µsec per phase with 53-msec interval) from behind the right ear of 12 normally hearing adults, and attempted to enhance it with (a) voluntary ear contraction alone, (b) head rotation alone (with the eyes forward-directed and tracking a target attached to the head), or (c) by lateral eye movement alone (toward the right measurement side, with the head facing forward)...
March 2014: Ear and Hearing
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